##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003810_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1905798 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65770086861252 31.0 31.0 34.0 30.0 34.0 2 31.887170098824743 31.0 31.0 34.0 30.0 34.0 3 31.910302665864904 31.0 31.0 34.0 30.0 34.0 4 35.69433119354727 37.0 35.0 37.0 33.0 37.0 5 35.567827755092615 37.0 35.0 37.0 33.0 37.0 6 35.595631331337316 37.0 35.0 37.0 33.0 37.0 7 35.520950279095686 37.0 35.0 37.0 33.0 37.0 8 35.51169483859255 37.0 35.0 37.0 33.0 37.0 9 37.07877644955027 39.0 37.0 39.0 33.0 39.0 10-11 37.00873439892371 39.0 37.0 39.0 33.0 39.0 12-13 36.95844549107513 39.0 37.0 39.0 33.0 39.0 14-15 38.08218079775506 40.0 37.0 41.0 33.0 41.0 16-17 38.07802873127163 40.0 37.0 41.0 33.0 41.0 18-19 38.09496022138758 40.0 37.0 41.0 33.0 41.0 20-21 38.03858777268105 40.0 37.0 41.0 33.0 41.0 22-23 37.94791997892746 40.0 37.0 41.0 33.0 41.0 24-25 37.85711051223687 40.0 37.0 41.0 33.0 41.0 26-27 37.64592653576087 39.5 37.0 41.0 32.0 41.0 28-29 37.44827888370121 39.0 36.0 41.0 32.0 41.0 30-31 37.19101263617655 39.0 36.0 40.5 31.5 41.0 32-33 36.91645730554865 39.0 35.5 40.0 31.0 41.0 34-35 36.88128726129422 39.0 35.0 40.0 31.0 41.0 36-37 36.68595963475667 39.0 35.0 40.0 30.0 41.0 38-39 36.41679443466727 39.0 35.0 40.0 30.0 41.0 40-41 36.47070649670112 39.0 35.0 40.0 30.0 41.0 42-43 36.31430665789344 39.0 35.0 41.0 30.0 41.0 44-45 36.1264879593745 39.0 35.0 41.0 30.0 41.0 46-47 35.80613947543234 39.0 35.0 40.5 28.5 41.0 48-49 35.52615466067233 39.0 35.0 40.0 27.5 41.0 50-51 35.26573828915761 38.5 35.0 40.0 26.5 41.0 52-53 35.048054673160536 38.0 34.0 40.0 26.0 41.0 54-55 34.82167128940213 38.0 34.0 40.0 26.0 41.0 56-57 34.581952809269396 38.0 34.0 40.0 25.0 41.0 58-59 34.31731720780482 38.0 34.0 40.0 24.0 41.0 60-61 33.91559703599227 37.5 33.0 40.0 23.0 41.0 62-63 33.659052008659884 37.0 33.0 40.0 22.5 41.0 64-65 33.21214236765911 36.5 32.0 39.5 21.5 41.0 66-67 32.874589804375915 36.0 32.0 39.0 21.0 41.0 68-69 32.61181825146212 35.5 32.0 39.0 20.0 40.0 70-71 32.20068811070218 35.0 31.0 38.5 19.5 40.0 72-73 31.84136986186364 35.0 31.0 38.0 18.0 40.0 74-75 31.456460495813303 35.0 31.0 37.0 17.5 39.5 76-77 30.479277447032686 34.0 29.5 36.0 15.0 39.0 78-79 30.844059811165717 34.0 31.0 36.0 14.0 39.0 80-81 30.671591113014077 34.0 31.0 36.0 12.0 38.5 82-83 30.373195637732856 34.0 30.5 35.5 8.5 37.0 84-85 30.020880229699056 34.0 30.0 35.0 7.0 37.0 86-87 29.646651953669803 34.0 30.0 35.0 3.5 36.0 88-89 29.330127327240348 34.0 29.5 35.0 2.0 36.0 90-91 29.052449944852498 34.0 29.0 35.0 2.0 36.0 92-93 28.79522646156623 34.0 29.0 35.0 2.0 35.5 94-95 28.483353954616387 34.0 29.0 35.0 2.0 35.0 96-97 28.1235178124859 34.0 29.0 35.0 2.0 35.0 98-99 27.743196288378936 34.0 29.0 35.0 2.0 35.0 100-101 26.786161230098887 33.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15174867229427846 1101 2 0.1044591751226811 1101 3 0.12777279989326473 1101 4 0.13286825905063893 1101 5 0.14493510734675397 1101 6 0.11020518834639148 1101 7 0.07832726093761266 1101 8 0.1249023271140004 1101 9 0.19035655070781132 1101 10-11 0.2682717636109828 1101 12-13 0.141192608284328 1101 14-15 0.10384788615623819 1101 16-17 0.08989122169229091 1101 18-19 0.02799554374573887 1101 20-21 0.07749114838153304 1101 22-23 0.0823887772267966 1101 24-25 0.15358452914320253 1101 26-27 0.2839267490073567 1101 28-29 0.14180450386227506 1101 30-31 0.24208044861129707 1101 32-33 0.1265768834841836 1101 34-35 0.18052882089677524 1101 36-37 0.15628875355345428 1101 38-39 0.008963617041999328 1101 40-41 -0.04092375926706637 1101 42-43 -0.08302271953743912 1101 44-45 -0.07124437291186325 1101 46-47 -0.16209464645934446 1101 48-49 -0.28656234570219397 1101 50-51 -0.2023778214952756 1101 52-53 -0.24607313340654002 1101 54-55 -0.21133997357078727 1101 56-57 -0.2676318052611819 1101 58-59 -0.4097535417890157 1101 60-61 -0.3694588887007484 1101 62-63 -0.542487774884016 1101 64-65 -0.5866612230593162 1101 66-67 -0.5416398789403658 1101 68-69 -0.5382823147729638 1101 70-71 -0.5106834838924357 1101 72-73 -0.5495364855984057 1101 74-75 -0.5812664809765309 1101 76-77 -0.6218959825730721 1101 78-79 -0.4513405844381957 1101 80-81 -0.35011019101512275 1101 82-83 -0.5802949111865168 1101 84-85 -0.8112705588022067 1101 86-87 -0.7488018582188118 1101 88-89 -0.6565321387448009 1101 90-91 -0.5461704442906203 1101 92-93 -0.3543425167119132 1101 94-95 -0.5218861440168752 1101 96-97 -0.4056477994749912 1101 98-99 -0.549958672669181 1101 100-101 -0.5065042048724919 1102 1 -0.0031550627570915424 1102 2 0.006736676364614169 1102 3 -0.021599514530006303 1102 4 0.03512703491781366 1102 5 -0.03956050265435351 1102 6 0.09435443398916732 1102 7 0.028974608157831483 1102 8 0.025244781173498154 1102 9 0.0536147769301607 1102 10-11 0.013930505772485446 1102 12-13 -0.03396082963941183 1102 14-15 -0.07723571272861562 1102 16-17 -0.1676177912711765 1102 18-19 -0.19288278097208433 1102 20-21 0.01213766287332163 1102 22-23 -4.197468511790703E-5 1102 24-25 -0.1559119069773871 1102 26-27 -0.09580691558153376 1102 28-29 0.10354883861028696 1102 30-31 -0.12805949052021504 1102 32-33 0.013028689326283427 1102 34-35 -0.08619087346105658 1102 36-37 -0.30769381422414455 1102 38-39 -0.287797223573115 1102 40-41 -0.09698477755294732 1102 42-43 -0.0419885391195578 1102 44-45 0.03230557261932887 1102 46-47 -0.032717865483441244 1102 48-49 -0.2504456414562739 1102 50-51 -0.1644611406009986 1102 52-53 -0.1715271411324082 1102 54-55 -0.2884772272632645 1102 56-57 -0.2080561993576211 1102 58-59 -0.35387647277278944 1102 60-61 -0.33311720458460314 1102 62-63 -0.44368016819618816 1102 64-65 -0.43075072374168855 1102 66-67 -0.5007170869649542 1102 68-69 -0.4983655986309188 1102 70-71 -0.5678868877249705 1102 72-73 -0.36770442246966084 1102 74-75 -0.30938133691781644 1102 76-77 -0.3763983337090977 1102 78-79 -0.37015272927566656 1102 80-81 -0.29848997796441523 1102 82-83 -0.35707577693769466 1102 84-85 -0.4435171411459429 1102 86-87 -0.5301145400339387 1102 88-89 -0.4167232862688124 1102 90-91 -0.3130236946301572 1102 92-93 -0.3669102722774831 1102 94-95 -0.13346170255624656 1102 96-97 -0.034757743860630796 1102 98-99 -0.1899923948661275 1102 100-101 -0.47605412947532244 1103 1 -0.09751145634898606 1103 2 -0.17137303632173584 1103 3 -0.045254689261806647 1103 4 0.00255942316860569 1103 5 -0.056345572000132904 1103 6 -0.04661014298479671 1103 7 -0.06331898159751148 1103 8 -0.06587517067098503 1103 9 -0.11726366400446864 1103 10-11 0.0878259997484605 1103 12-13 0.054125110844530866 1103 14-15 0.051503138322345876 1103 16-17 -0.15185541551760906 1103 18-19 -0.06453641570517021 1103 20-21 -0.006521071577729742 1103 22-23 -0.018748070207813328 1103 24-25 0.02470106751180623 1103 26-27 -0.026202181918812073 1103 28-29 -0.24826936820787182 1103 30-31 -0.09222029816399413 1103 32-33 -0.08418174355391272 1103 34-35 -0.1307875862971244 1103 36-37 -0.18623313766359217 1103 38-39 -0.20276271110187594 1103 40-41 -0.064922401493817 1103 42-43 -0.08214476954492511 1103 44-45 -0.15883225421026026 1103 46-47 -0.16139591102951556 1103 48-49 -0.05459340188868822 1103 50-51 -0.04274165870056379 1103 52-53 -0.07884839892025042 1103 54-55 -0.34573248510610455 1103 56-57 -0.42137718825679116 1103 58-59 -0.42406481706028387 1103 60-61 -0.3772531400268804 1103 62-63 -0.5279857784970758 1103 64-65 -0.5101098520535245 1103 66-67 -0.6087941357896369 1103 68-69 -0.46685826377592576 1103 70-71 -0.48827475962396605 1103 72-73 -0.4450031913873538 1103 74-75 -0.36030930836673036 1103 76-77 -0.37513852920337953 1103 78-79 -0.3602987235984507 1103 80-81 -0.4076219564655439 1103 82-83 -0.48156465930807 1103 84-85 -0.8311435264572893 1103 86-87 -0.6930393764854621 1103 88-89 -0.6514701184242284 1103 90-91 -0.5508688575369654 1103 92-93 -0.6657236482663329 1103 94-95 -0.9028039642154724 1103 96-97 -0.6673427722619927 1103 98-99 -0.7898257144494742 1103 100-101 -0.7914458666488748 1104 1 -0.07737614853343544 1104 2 -0.17000763386855056 1104 3 -0.10504828970807623 1104 4 -0.05481018251873593 1104 5 -0.055880491924632736 1104 6 0.06361513220379322 1104 7 -0.08425536876207929 1104 8 -0.18419880672799138 1104 9 -0.07406959523488865 1104 10-11 -0.12152003409795498 1104 12-13 -0.13822701379952207 1104 14-15 -0.21617574237196635 1104 16-17 -0.21057193721161127 1104 18-19 -0.16378398489960944 1104 20-21 -0.16122830464316706 1104 22-23 -0.15749047292694485 1104 24-25 -0.23816647223405596 1104 26-27 -0.21436015346914417 1104 28-29 -0.19424905257611869 1104 30-31 -0.3698513735562585 1104 32-33 -0.18142758734668263 1104 34-35 -0.04311999206489503 1104 36-37 -0.1932252306536668 1104 38-39 -0.33725160950425703 1104 40-41 -0.1434336911991423 1104 42-43 0.08934102183714998 1104 44-45 0.18726645692765942 1104 46-47 0.15146708818561905 1104 48-49 0.07136513657933108 1104 50-51 0.00805228552874837 1104 52-53 0.10675714571640782 1104 54-55 -0.023530587164259487 1104 56-57 -0.21498379667494305 1104 58-59 -0.09840822388535031 1104 60-61 -0.012547982632625576 1104 62-63 0.07133865539604756 1104 64-65 -0.029039648962076114 1104 66-67 -0.03879504389578159 1104 68-69 -0.1913550756747 1104 70-71 -0.06550172778187147 1104 72-73 0.013127926166987436 1104 74-75 -0.12437100769278686 1104 76-77 -0.30364917026145477 1104 78-79 -0.2369052606653952 1104 80-81 -0.2604208693327621 1104 82-83 -0.4045546636540074 1104 84-85 -0.3450239288780388 1104 86-87 -0.3722015837023136 1104 88-89 -0.3098928029157264 1104 90-91 -0.17698404431493842 1104 92-93 -0.36101544223002335 1104 94-95 -0.3708269522738803 1104 96-97 -0.24268314990442263 1104 98-99 -0.1431811666538998 1104 100-101 0.008391647215958642 1105 1 0.004353604838112091 1105 2 0.11144004310151345 1105 3 0.07823635626313674 1105 4 0.11327425422241788 1105 5 -0.04827890969617954 1105 6 0.13023037792351744 1105 7 0.10054900260945487 1105 8 0.15783619202342436 1105 9 0.04068629564040549 1105 10-11 0.18630341178519672 1105 12-13 0.09437334939785558 1105 14-15 0.16039108195604967 1105 16-17 0.15671732186869036 1105 18-19 0.20917930970547616 1105 20-21 0.16304134798469505 1105 22-23 0.1773614775966479 1105 24-25 0.12366928512408748 1105 26-27 0.21128227590462956 1105 28-29 0.251738266545523 1105 30-31 0.29767674173304925 1105 32-33 0.22297747377479737 1105 34-35 0.18643205465132695 1105 36-37 0.28529359488008765 1105 38-39 0.29258554272094983 1105 40-41 0.33231054573913354 1105 42-43 0.409381744862948 1105 44-45 0.385138471963856 1105 46-47 0.3520161693317192 1105 48-49 0.2246553383769978 1105 50-51 0.16442737473965963 1105 52-53 0.29388026194400396 1105 54-55 0.32299229294071097 1105 56-57 0.2088869742107562 1105 58-59 0.21183924680988042 1105 60-61 0.18995450345195053 1105 62-63 0.09168243693385847 1105 64-65 0.014279913014206613 1105 66-67 -0.06621511609197483 1105 68-69 0.12199390558472345 1105 70-71 0.1755801364343199 1105 72-73 0.08294109672642591 1105 74-75 0.094805976409571 1105 76-77 0.14824453909494295 1105 78-79 0.15237605772039942 1105 80-81 0.234399726234102 1105 82-83 0.18870342960408593 1105 84-85 0.029520042680857728 1105 86-87 0.2719421910384767 1105 88-89 0.3280752865449017 1105 90-91 0.35771696799801234 1105 92-93 0.49563795546815115 1105 94-95 0.4864692072610879 1105 96-97 0.5224790107801276 1105 98-99 0.46532774409381616 1105 100-101 0.5345897331155385 1106 1 -0.13063358528926727 1106 2 -0.1276378866306196 1106 3 0.0017752489609073052 1106 4 0.001519241532825788 1106 5 0.052303247895324034 1106 6 -0.007690453455253987 1106 7 0.0773853850533115 1106 8 0.03880300663509928 1106 9 0.06632047454561274 1106 10-11 -0.06593079839294091 1106 12-13 -0.018088311194645712 1106 14-15 0.013420346200270217 1106 16-17 -0.03684406328147105 1106 18-19 0.029016570196937153 1106 20-21 0.036158056148131834 1106 22-23 0.035241052765947245 1106 24-25 -0.15914443647468346 1106 26-27 -0.10309906137390357 1106 28-29 -0.005511038834619342 1106 30-31 -0.04050690252536526 1106 32-33 -0.03610370353067793 1106 34-35 0.04655546243828468 1106 36-37 -0.09433810624693706 1106 38-39 0.050672000340121315 1106 40-41 -0.1868850183162465 1106 42-43 -0.2426997090876668 1106 44-45 -0.30079592705465785 1106 46-47 -0.15873209562214896 1106 48-49 -0.18248030193621645 1106 50-51 -0.2418915610185266 1106 52-53 -0.20272241549979242 1106 54-55 -0.314368632737974 1106 56-57 -0.24511656203576848 1106 58-59 -0.35567419453533233 1106 60-61 -0.3701725127498321 1106 62-63 -0.5305598320373903 1106 64-65 -0.5492618664154207 1106 66-67 -0.4645943364338123 1106 68-69 -0.563589875793447 1106 70-71 -0.4718272175150915 1106 72-73 -0.481816117096205 1106 74-75 -0.5192379740829765 1106 76-77 -0.5430249012399173 1106 78-79 -0.38931556714879534 1106 80-81 -0.5531602636623347 1106 82-83 -0.40123570846589374 1106 84-85 -0.25356551283460504 1106 86-87 -0.13464136180675723 1106 88-89 -0.231352899986085 1106 90-91 -0.45988728512026 1106 92-93 -0.5323507141093025 1106 94-95 -0.3584748021689066 1106 96-97 -0.34871982490468767 1106 98-99 -0.4468007395299942 1106 100-101 -0.4944766496891617 1107 1 -0.17033167305358177 1107 2 -0.040484139884792825 1107 3 -0.05565175457888216 1107 4 0.0639194313204996 1107 5 -0.0438878882710938 1107 6 -0.12785851543591775 1107 7 -0.17677527271081317 1107 8 -0.036595141686468935 1107 9 -0.0405237434854655 1107 10-11 0.03788430845627033 1107 12-13 0.11248873073969179 1107 14-15 -0.03317752243419392 1107 16-17 -0.1349187172623374 1107 18-19 -0.051123148492770554 1107 20-21 0.03055307671886709 1107 22-23 -0.07066546431806842 1107 24-25 -0.12571558500226132 1107 26-27 -0.043499488396172126 1107 28-29 -0.20726532622560256 1107 30-31 0.042515840780566805 1107 32-33 -0.008478359384447742 1107 34-35 -0.21821894619206006 1107 36-37 -0.0380248566948751 1107 38-39 -0.009109868693897738 1107 40-41 0.077796369381268 1107 42-43 0.02939525966604606 1107 44-45 0.011550884949414808 1107 46-47 -0.05121113469719063 1107 48-49 -0.12458819373227215 1107 50-51 0.005411144080142094 1107 52-53 -0.014524836541248476 1107 54-55 -0.33057674990689634 1107 56-57 -0.21321849799492298 1107 58-59 -0.05625292208389254 1107 60-61 0.03262310964760928 1107 62-63 -0.09338267398295841 1107 64-65 -0.1085655204524727 1107 66-67 -0.01843035738536969 1107 68-69 -0.12047623418001052 1107 70-71 -0.12540665052441113 1107 72-73 -0.0796872487676481 1107 74-75 -0.1012618348887031 1107 76-77 -0.12432956664549621 1107 78-79 -0.18025445426883024 1107 80-81 -0.17899011651445562 1107 82-83 -0.11868805864912346 1107 84-85 -0.0918327014173741 1107 86-87 -0.08464292256066486 1107 88-89 -0.251406714780785 1107 90-91 -0.07558722081719793 1107 92-93 -0.2556090688249668 1107 94-95 -0.13268802612460462 1107 96-97 0.013606697298595094 1107 98-99 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-0.04498209870092751 2316 70-71 -0.3244989128833069 2316 72-73 -0.30401119276393374 2316 74-75 -0.1400936440230467 2316 76-77 -0.10151439315129807 2316 78-79 0.047382884706923534 2316 80-81 -0.14534600934919695 2316 82-83 -0.07616731496208828 2316 84-85 -0.25235577802745013 2316 86-87 -0.06352318732030326 2316 88-89 -0.005377600715092967 2316 90-91 -0.11655901211553044 2316 92-93 0.04217448474579655 2316 94-95 -0.06601627314317682 2316 96-97 0.09035460936638628 2316 98-99 -0.11330215484064965 2316 100-101 -0.6845457548016753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 21.0 5 49.0 6 119.0 7 306.0 8 749.0 9 1425.0 10 2431.0 11 4247.0 12 6869.0 13 9453.0 14 10304.0 15 12837.0 16 15743.0 17 20531.0 18 17804.0 19 12105.0 20 10677.0 21 10409.0 22 11164.0 23 12417.0 24 14574.0 25 17618.0 26 21581.0 27 26963.0 28 33819.0 29 42466.0 30 53061.0 31 65484.0 32 80787.0 33 101995.0 34 132130.0 35 175664.0 36 246106.0 37 341091.0 38 322247.0 39 70517.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.109308999045574 20.66776494354794 14.181053988813536 21.041872068592955 2 15.838567657908683 21.686580713547325 39.99092192329287 22.483929705251114 3 19.28958980268425 27.711300047511177 30.14014484346249 22.858965306342085 4 12.924979457424135 17.81998931681112 38.638984824204876 30.61604640155987 5 12.114085543168793 38.36917658639583 34.6976437167003 14.819094153735076 6 28.251236373653764 38.80006821371883 18.60660361270349 14.342091799923914 7 26.7443349190208 33.17607637325677 22.80545997004929 17.27412873767314 8 25.535182637404386 34.69071748422445 20.60186861356765 19.17223126480351 9 25.286310511397325 18.08586219525889 22.63172697211352 33.996100321230266 10-11 24.12513970584377 27.086772414589227 28.43626527581738 20.351822603749625 12-13 25.626325560211523 24.84373999762829 28.723610791909742 20.806323650250448 14-15 22.29441908837799 26.771825836181733 27.74349257456135 23.190262500878923 16-17 20.49209832311714 29.67958828795077 28.55008767980657 21.278225709125522 18-19 20.22520225123544 28.717051859640947 31.200709624000023 19.857036265123586 20-21 21.48821439095114 27.609599355152053 30.759582916462332 20.14260333743448 22-23 20.87831449083271 27.683023069601294 30.73211326698842 20.706549172577578 24-25 21.19225122494619 27.79840780607389 30.928592642032367 20.080748326947557 26-27 21.07246328138406 27.3223025743044 31.42842749500037 20.176806649311178 28-29 20.73972637964018 28.0127246245467 31.36390626242229 19.88364273339083 30-31 21.280054198181254 28.001011755358814 30.734981494007922 19.98395255245201 32-33 20.899742296577756 28.067318787686695 30.900849513480498 20.132089402255048 34-35 21.34133302617759 27.344461082047054 31.116520737079323 20.197685154696032 36-37 21.668700387062913 27.67434232106541 30.854057600209934 19.802899691661747 38-39 21.42272957338264 28.095173997885688 30.878599652022597 19.603496776709076 40-41 20.9442499513257 28.115614453354798 30.678139282496197 20.261996312823307 42-43 20.757184129692654 28.466579354160302 30.107834093644765 20.66840242250228 44-45 21.162473672445874 28.283217843653947 29.94100109245576 20.613307391444422 46-47 21.508519274885664 28.68302798410743 29.446882037741712 20.361570703265198 48-49 21.629128585505914 28.565278166941095 29.45553516164882 20.35005808590417 50-51 21.303149651746935 28.79240087354484 29.425547723315905 20.478901751392332 52-53 21.302703644352654 29.24394400665758 28.811946491705836 20.64140585728393 54-55 21.153842117580144 29.398052679245122 28.718757182030835 20.7293480211439 56-57 21.621769819747964 28.82033355441563 28.640984040097052 20.916912585739354 58-59 21.907584878097925 28.244818951910805 28.892974679293438 20.954621490697832 60-61 21.701974247305422 28.90804775641311 29.215617025095153 20.174360971186307 62-63 21.384214680512958 28.86553708963354 29.06154945809271 20.688698771760784 64-65 21.12066668853965 29.268020604350536 28.94898126578956 20.66233144132025 66-67 21.247566063977747 28.49161571364841 28.82627863647099 21.434539585902854 68-69 21.571672905192166 29.122890513024817 28.60435872094442 20.7010778608386 70-71 21.748671154744226 28.716621453570433 28.467564631989884 21.067142759695454 72-73 21.469405466896283 28.183836901917203 29.249322331117988 21.09743530006853 74-75 21.08238152269588 28.997230818069603 29.256754718169166 20.663632941065355 76-77 21.370811597031793 28.84846662657847 28.640994481052033 21.1397272953377 78-79 21.107063812639115 29.108331523068028 28.56821656859751 21.216388095695347 80-81 21.392079374686645 29.267894027510284 28.282085731608802 21.057940866194265 82-83 20.906675313962968 28.992448307743 28.88999778570447 21.21087859258956 84-85 20.80265170108755 28.83709191253731 28.44914367779754 21.911112708577598 86-87 21.1446989036014 28.690303470748272 28.15123426829364 22.01376335735668 88-89 21.25852267659007 28.497485043010855 28.354893855487305 21.88909842491177 90-91 21.043835705567957 27.33427152300506 28.668883061058935 22.953009710368043 92-93 20.687003554416574 27.81380817904101 28.848597805223847 22.650590461318565 94-95 20.470144388372844 27.915688838924385 29.259850535012244 22.354316237690522 96-97 19.89285833283704 28.50606400637634 29.458763785917107 22.142313874869508 98-99 19.952644310233552 29.278304535127162 28.392110358434035 22.376940796205247 100-101 19.23252906029503 30.24891658476665 27.73120597928721 22.787348375651113 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 456.0 1 354.5 2 663.5 3 902.5 4 604.5 5 514.0 6 659.0 7 1070.5 8 1549.5 9 1796.0 10 1858.5 11 1897.0 12 2031.5 13 2248.5 14 2526.0 15 2819.5 16 3069.5 17 3328.0 18 3720.5 19 4015.0 20 4380.5 21 4942.0 22 5599.0 23 6556.0 24 7684.0 25 9052.0 26 11161.0 27 14289.5 28 18421.5 29 23311.5 30 28486.0 31 33525.5 32 38462.5 33 42984.5 34 47025.5 35 51181.0 36 55417.5 37 59935.5 38 64003.0 39 66356.5 40 68340.0 41 70127.0 42 72277.0 43 75476.5 44 76665.0 45 77397.0 46 79477.5 47 84815.5 48 99460.0 49 97421.5 50 81064.5 51 70486.0 52 58274.5 53 49235.0 54 42738.5 55 37400.0 56 31801.0 57 25523.5 58 20286.0 59 16218.5 60 12735.5 61 9668.5 62 7179.5 63 4930.5 64 3190.0 65 2063.0 66 1273.0 67 741.5 68 387.0 69 198.0 70 125.5 71 65.5 72 38.0 73 31.0 74 21.5 75 11.5 76 7.0 77 4.0 78 3.5 79 3.0 80 2.0 81 2.0 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05226157231773777 2 0.005666917480236625 3 0.051579443361783356 4 0.0 5 0.0 6 0.0012068435374577998 7 0.0 8 0.0 9 0.0 10-11 4.19771665202713E-4 12-13 0.0 14-15 0.0027285158238176343 16-17 0.0 18-19 0.0 20-21 0.012199614019953847 22-23 0.0 24-25 0.0 26-27 0.008238018929603243 28-29 0.010572998817293334 30-31 0.010441820171917485 32-33 0.006244103519890356 34-35 0.007424711328272986 36-37 0.02219542679759345 38-39 0.011255127773247742 40-41 0.014429650991343258 42-43 0.0 44-45 0.0 46-47 1.0494291630067825E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0011543720793074606 58-59 3.1482874890203475E-4 60-61 0.011071477669721554 62-63 0.01605626619400377 64-65 0.045151689738366814 66-67 0.023507213251351928 68-69 0.0017053223898860215 70-71 4.19771665202713E-4 72-73 0.0 74-75 2.6235729075169562E-5 76-77 0.0 78-79 0.0 80-81 7.870718722550869E-5 82-83 0.0 84-85 1.311786453758478E-4 86-87 6.821289559544086E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 2.6235729075169562E-5 96-97 1.8365010352618694E-4 98-99 4.19771665202713E-4 100-101 9.969577048564433E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1905798.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.65843715946284 #Duplication Level Percentage of deduplicated Percentage of total 1 80.7214890118602 48.15717879632332 2 12.346762958436251 14.731771641573053 3 3.023028025846842 5.410473825338364 4 1.1811780279144528 2.8186894060989047 5 0.6514873814090093 1.943335950198619 6 0.404357613088669 1.447400595024045 7 0.26906491370134816 1.123639457210772 8 0.19909418770084605 0.9502118468612177 9 0.15029253633625517 0.8069596051097487 >10 0.9281460393871361 10.51868343325778 >50 0.07887066688946237 3.252399874662542 >100 0.042051248746332245 4.459249415656089 >500 0.0018664928158753013 0.6984424420804777 >1k 0.0020442540364348535 2.3918949377321788 >5k 1.7776122055955247E-4 0.7409152932880742 >10k+ 8.888061027977623E-5 0.5487534795847906 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10349 0.5430271203978596 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7539 0.3955823229954067 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6434 0.33760136173928196 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3882 0.20369420053961648 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3291 0.17268356877276606 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2988 0.1567847169532133 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2963 0.15547293049945482 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2824 0.1481793978165577 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2398 0.1258265566445132 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2262 0.1186904383360671 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2244 0.117745952089361 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2104 0.11039994794831351 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2028 0.10641211712888775 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2013 0.10562504525663266 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1993 0.10457561609362588 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.2471458150339125E-5 0.0 10-11 0.0 0.0 0.0 5.2471458150339125E-5 0.0 12-13 0.0 0.0 0.0 5.2471458150339125E-5 0.0 14-15 0.0 0.0 0.0 1.311786453758478E-4 0.0 16-17 0.0 0.0 0.0 3.1482874890203475E-4 0.0 18-19 0.0 0.0 0.0 4.19771665202713E-4 0.0 20-21 0.0 0.0 0.0 8.39543330405426E-4 0.0 22-23 0.0 0.0 0.0 0.001679086660810852 0.0 24-25 0.0 0.0 0.0 0.0030171088436445 0.0 26-27 0.0 2.6235729075169562E-5 0.0 0.0054570316476352685 0.0 28-29 0.0 5.2471458150339125E-5 0.0 0.012855507246833086 0.0 30-31 0.0 5.2471458150339125E-5 0.0 0.028885537711761687 0.0 32-33 0.0 5.2471458150339125E-5 0.0 0.051054728780279966 0.0 34-35 0.0 5.2471458150339125E-5 0.0 0.06745205945226095 0.0 36-37 0.0 1.0494291630067825E-4 0.0 0.08943760041725304 0.0 38-39 0.0 1.5741437445101737E-4 0.0 0.12805659361590263 0.0 40-41 0.0 1.5741437445101737E-4 0.0 0.18079040905699345 0.0 42-43 0.0 1.5741437445101737E-4 0.0 0.22405312630194807 0.0 44-45 0.0 1.5741437445101737E-4 0.0 0.2391911419783209 0.0 46-47 0.0 1.5741437445101737E-4 0.0 0.2624884693970715 0.0 48-49 0.0 1.5741437445101737E-4 0.0 0.296148909800514 0.0 50-51 0.0 1.5741437445101737E-4 0.0 0.3278154347942437 0.0 52-53 0.0 1.5741437445101737E-4 0.0 0.4075195797246088 0.0 54-55 0.0 1.5741437445101737E-4 0.0 0.4470830591699645 0.0 56-57 0.0 1.5741437445101737E-4 0.0 0.5645141825104234 0.0 58-59 0.0 1.5741437445101737E-4 0.0 0.8128091224778282 0.0 60-61 0.0 1.5741437445101737E-4 0.0 0.981898396367296 0.0 62-63 0.0 1.5741437445101737E-4 0.0 1.0621272558791646 0.0 64-65 0.0 1.5741437445101737E-4 0.0 1.2457773594053516 0.0 66-67 0.0 1.8365010352618694E-4 0.0 1.7648512591575813 0.0 68-69 0.0 2.098858326013565E-4 0.0 2.3200517578463193 0.0 70-71 0.0 2.098858326013565E-4 0.0 2.6770675591012267 0.0 72-73 0.0 2.098858326013565E-4 0.0 3.064228213063504 0.0 74-75 0.0 2.098858326013565E-4 0.0 3.5083466348479746 0.0 76-77 0.0 2.098858326013565E-4 0.0 3.683548833611957 0.0 78-79 0.0 2.098858326013565E-4 0.0 3.9366449120001175 0.0 80-81 0.0 2.098858326013565E-4 0.0 4.281959578087499 0.0 82-83 0.0 2.098858326013565E-4 0.0 4.606154482269369 0.0 84-85 0.0 2.098858326013565E-4 0.0 5.212383474009313 0.0 86-87 0.0 2.098858326013565E-4 0.0 6.488725457787236 0.0 88-89 0.0 2.098858326013565E-4 0.0 8.008325121550133 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2635 0.0 58.437466 2 TATAACG 465 0.0 54.168884 2 ACCTGGG 3035 0.0 49.634308 3 TAACGCA 545 0.0 46.184753 4 GTACCTG 3505 0.0 44.219746 1 ATAACGC 585 0.0 43.0528 3 CCTGGGG 2215 0.0 36.66415 4 GTATAAC 780 0.0 36.571545 1 TCACGCA 360 0.0 34.29968 4 GTACACG 310 0.0 33.7402 1 TATCACG 375 0.0 30.41634 2 CACGCAG 400 0.0 29.682417 5 GTACCGG 235 0.0 28.323496 1 GTACCCG 210 4.1836756E-11 27.167435 1 TACACGG 335 0.0 26.95478 2 CTGGGGG 1445 0.0 26.621737 5 TACCGGG 240 5.8971636E-9 21.782536 2 GTACGAC 110 0.0028708419 21.600246 3 GGTCGCG 155 3.914113E-5 21.447378 7 TACCGAC 135 3.802277E-4 21.106943 7 >>END_MODULE