##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003809_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2142569 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.664541958742053 31.0 31.0 34.0 30.0 34.0 2 31.86296170625077 31.0 31.0 34.0 30.0 34.0 3 31.94021102704277 31.0 31.0 34.0 30.0 34.0 4 35.56599577423177 37.0 35.0 37.0 33.0 37.0 5 35.49437474359052 37.0 35.0 37.0 33.0 37.0 6 35.58191404804233 37.0 35.0 37.0 33.0 37.0 7 35.525848175718025 37.0 35.0 37.0 33.0 37.0 8 35.547441879351375 37.0 35.0 37.0 33.0 37.0 9 37.09012218509649 39.0 37.0 39.0 33.0 39.0 10-11 36.988718216309486 39.0 37.0 39.0 33.0 39.0 12-13 36.98111916115654 39.0 37.0 39.0 33.0 39.0 14-15 37.911324909489494 39.5 37.0 41.0 33.0 41.0 16-17 38.06148273404497 40.0 37.0 41.0 33.0 41.0 18-19 38.127745710873256 40.0 37.0 41.0 33.0 41.0 20-21 38.03065945600818 40.0 37.0 41.0 33.0 41.0 22-23 37.96012077090633 40.0 37.0 41.0 33.0 41.0 24-25 37.77484039020447 39.5 37.0 41.0 32.5 41.0 26-27 37.437156516312896 39.0 36.5 41.0 31.5 41.0 28-29 37.349324339146136 39.0 36.0 41.0 31.5 41.0 30-31 37.21172386980302 39.0 36.0 40.0 31.0 41.0 32-33 36.966202021965216 39.0 36.0 40.0 31.0 41.0 34-35 36.84438984228746 39.0 35.0 40.0 30.0 41.0 36-37 36.60822521935116 38.5 35.5 40.0 30.5 41.0 38-39 36.42660399735084 38.0 35.0 40.0 30.0 41.0 40-41 36.76840022421682 39.0 35.0 40.5 30.0 41.0 42-43 36.62636535859522 39.0 35.0 41.0 30.0 41.0 44-45 36.49537634493918 39.0 35.0 41.0 30.0 41.0 46-47 36.22340820762365 39.0 35.0 41.0 30.0 41.0 48-49 35.91279977447634 39.0 35.0 40.0 28.0 41.0 50-51 35.510478075618565 38.0 34.5 40.0 27.5 41.0 52-53 35.51122834317121 38.0 34.5 40.0 28.0 41.0 54-55 35.35098869628003 38.0 34.0 40.0 27.0 41.0 56-57 34.94624257141777 38.0 34.0 40.0 26.5 41.0 58-59 34.6875953586559 38.0 34.0 40.0 26.0 41.0 60-61 34.004226935048536 37.0 33.0 40.0 24.0 41.0 62-63 33.996637447848826 37.0 33.0 40.0 25.0 41.0 64-65 33.57876269095651 36.5 32.5 39.5 23.5 41.0 66-67 33.11460167677214 36.0 32.0 39.0 22.0 40.0 68-69 32.741798047110734 36.0 32.0 39.0 22.0 40.0 70-71 32.13146391084721 35.0 31.0 37.5 21.5 40.0 72-73 31.643227592670293 35.0 30.5 37.0 20.5 39.5 74-75 31.327313612770464 34.0 31.0 37.0 20.0 39.0 76-77 30.436795267737004 34.0 29.5 35.5 19.0 38.0 78-79 30.82709565012842 34.0 30.0 36.0 18.5 38.0 80-81 30.47847070502747 34.0 30.0 35.5 18.0 37.0 82-83 30.109664846266327 34.0 29.5 35.0 14.5 37.0 84-85 29.83327654791981 34.0 29.5 35.0 11.0 36.5 86-87 29.718833325787877 34.0 30.0 35.0 7.5 36.0 88-89 29.370606267522774 34.0 29.0 35.0 6.0 36.0 90-91 29.027079407944388 34.0 29.0 35.0 2.0 35.0 92-93 28.759516729682918 34.0 29.0 35.0 2.0 35.0 94-95 28.366531486267185 34.0 29.0 35.0 2.0 35.0 96-97 27.830880359045615 34.0 28.0 35.0 2.0 35.0 98-99 27.596224205614845 34.0 28.5 35.0 2.0 35.0 100-101 26.551631942775238 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.0015596321871349517 1101 2 0.12125300358761848 1101 3 0.09589668689736541 1101 4 0.16098307080126517 1101 5 0.15581706409562912 1101 6 0.14248968348179147 1101 7 0.13352241911434248 1101 8 0.14731191253045495 1101 9 0.2184683486558754 1101 10-11 0.2624538956529179 1101 12-13 0.20651258045709397 1101 14-15 0.2623021259737257 1101 16-17 0.25615816986604045 1101 18-19 0.05672247849045675 1101 20-21 0.13424328683356634 1101 22-23 0.10368554877535274 1101 24-25 0.004813817043178403 1101 26-27 0.16597575762465766 1101 28-29 0.1455958585608883 1101 30-31 0.1345538010083942 1101 32-33 0.2168925537150912 1101 34-35 0.19031222397374137 1101 36-37 0.16320720096243235 1101 38-39 0.14367010710378025 1101 40-41 0.18391107083817815 1101 42-43 -0.00849794541149862 1101 44-45 -0.029012266238119366 1101 46-47 -0.05288048176579707 1101 48-49 0.025133651687411884 1101 50-51 0.03780792732919025 1101 52-53 -0.03503302501820116 1101 54-55 0.058469116387534825 1101 56-57 0.01926777100982946 1101 58-59 0.008939684520704816 1101 60-61 -0.012742084184488078 1101 62-63 -0.15485815096544542 1101 64-65 -0.22906014416781062 1101 66-67 -0.21345683325071008 1101 68-69 -0.35841158758974956 1101 70-71 -0.19109502295901848 1101 72-73 -0.10935961445597897 1101 74-75 -0.26232973133615545 1101 76-77 -0.2005044299236367 1101 78-79 -0.10334676274869281 1101 80-81 -8.760497714810356E-4 1101 82-83 -0.03417870768903697 1101 84-85 -0.06945583265683197 1101 86-87 -0.20008095076151378 1101 88-89 -0.12734155090281973 1101 90-91 -0.19955792164453712 1101 92-93 -0.03520960940142359 1101 94-95 -0.1656954431669817 1101 96-97 -0.06749226900354444 1101 98-99 -0.45157391835363825 1101 100-101 -0.2803470003951176 1102 1 0.06966381324759396 1102 2 0.1727285422172855 1102 3 0.04301623016748124 1102 4 0.16083765535575623 1102 5 0.009025447181386426 1102 6 0.16044489766528613 1102 7 0.04249739618961712 1102 8 0.11340671881368536 1102 9 0.14997874417824164 1102 10-11 0.13789218713305473 1102 12-13 0.1269298111629027 1102 14-15 0.0742814075602709 1102 16-17 0.048632591809067094 1102 18-19 0.01708835965377631 1102 20-21 0.05898330129559781 1102 22-23 0.0889949189888597 1102 24-25 0.006647947714938596 1102 26-27 0.06049168397991167 1102 28-29 0.22816560789738105 1102 30-31 0.1141565706603842 1102 32-33 0.11122040331117233 1102 34-35 0.07501444265258073 1102 36-37 0.05012543787890422 1102 38-39 -0.019061703235941252 1102 40-41 -0.05604375709987863 1102 42-43 0.2225245361833501 1102 44-45 0.11977036488845982 1102 46-47 0.05501357383122496 1102 48-49 0.020179052346719573 1102 50-51 0.2865030492449776 1102 52-53 0.061441713138435716 1102 54-55 0.001692794853958901 1102 56-57 0.17313160924668836 1102 58-59 -0.03245463400150328 1102 60-61 0.03325353989625057 1102 62-63 -0.15288930840358717 1102 64-65 -0.26776809964652415 1102 66-67 -0.17238660309522658 1102 68-69 -0.32744138521763233 1102 70-71 -0.14567234490804992 1102 72-73 -0.10302876725274857 1102 74-75 -0.06267447756479427 1102 76-77 -0.21489719505414584 1102 78-79 -0.16636639169312417 1102 80-81 -0.04674743615561283 1102 82-83 -0.11658216964699264 1102 84-85 -0.23685630450013662 1102 86-87 -0.3776761768534804 1102 88-89 -0.23038460228462654 1102 90-91 -0.20718588685431527 1102 92-93 -0.09928636734953855 1102 94-95 -0.1043819609533081 1102 96-97 -0.034797647402516674 1102 98-99 -0.154587565539682 1102 100-101 -0.22444951721384498 1103 1 -0.04070638942404514 1103 2 -0.01942623532648824 1103 3 -0.019899755085177162 1103 4 0.005323836925157366 1103 5 0.056002054201364615 1103 6 -0.02693824417705315 1103 7 -0.022699402132687396 1103 8 0.04043311249770909 1103 9 -0.010274935457381673 1103 10-11 0.0337046063935631 1103 12-13 0.034292259864464825 1103 14-15 -0.0455948307762597 1103 16-17 -0.1209558390967942 1103 18-19 0.03263552144652948 1103 20-21 0.08185944927340216 1103 22-23 -0.068128476274957 1103 24-25 -0.21428757289529443 1103 26-27 -0.07652341982835509 1103 28-29 -0.1113838595322747 1103 30-31 -0.0488940093731145 1103 32-33 0.028652636750457816 1103 34-35 -0.11798186256925902 1103 36-37 -0.03736538971764247 1103 38-39 -0.12840196539540472 1103 40-41 -0.001847140487974741 1103 42-43 0.0819911516136429 1103 44-45 0.08937508022372498 1103 46-47 0.009320110341612065 1103 48-49 0.03975412803456635 1103 50-51 0.13803199829357737 1103 52-53 0.09346469602886032 1103 54-55 -0.07154245458080055 1103 56-57 0.02016198467229202 1103 58-59 -0.017890992167600928 1103 60-61 0.13923836558831937 1103 62-63 -0.19214520927510392 1103 64-65 -0.11059133315829683 1103 66-67 -0.3805343783343531 1103 68-69 -0.34293790640675326 1103 70-71 -0.33593827758384975 1103 72-73 -0.187718474401116 1103 74-75 -0.24530782867198653 1103 76-77 -0.26712180698328325 1103 78-79 -0.22781131705780666 1103 80-81 -0.3033791210876515 1103 82-83 -0.3781046446419545 1103 84-85 -0.48913984819056466 1103 86-87 -0.4230074607317924 1103 88-89 -0.31585675640682 1103 90-91 -0.39375257308215694 1103 92-93 -0.5195219553812791 1103 94-95 -0.8123068182411366 1103 96-97 -0.576748174511625 1103 98-99 -0.4311108299322832 1103 100-101 -0.3814780854649662 1104 1 0.05127105468431381 1104 2 0.050879802721485845 1104 3 0.018318273125785822 1104 4 0.038772284195125906 1104 5 0.005578326240552656 1104 6 0.0012274393924442961 1104 7 -0.002741623992662312 1104 8 -0.02431747061877587 1104 9 -0.038306789013134335 1104 10-11 -8.567430959658395E-4 1104 12-13 -0.049183456528602676 1104 14-15 0.19892518234512124 1104 16-17 0.06301766457296765 1104 18-19 -0.06378900345691818 1104 20-21 -0.06376068681059621 1104 22-23 -0.05366628174447641 1104 24-25 -0.04269636421907563 1104 26-27 0.10619731416386458 1104 28-29 0.07052785693324637 1104 30-31 0.001028107596326322 1104 32-33 0.015847217540517988 1104 34-35 0.0731097878466187 1104 36-37 0.08175439116337913 1104 38-39 -0.09780268868122022 1104 40-41 0.06263729196208345 1104 42-43 0.2679811616559249 1104 44-45 0.3888227547008327 1104 46-47 0.2682640808466843 1104 48-49 0.3176642264650269 1104 50-51 0.42026993799561296 1104 52-53 0.3941329404809508 1104 54-55 0.29455105738038156 1104 56-57 0.16656547914501374 1104 58-59 0.25994967565422655 1104 60-61 0.4102320685339862 1104 62-63 0.3997280889203054 1104 64-65 0.2591699261720706 1104 66-67 0.35987715398912457 1104 68-69 0.2901102583108255 1104 70-71 0.33699274059086903 1104 72-73 0.41295037379540034 1104 74-75 0.3025791664718973 1104 76-77 0.2882782843833702 1104 78-79 0.4841125346706079 1104 80-81 0.3765518105503709 1104 82-83 0.5401272294411079 1104 84-85 0.5203042239448195 1104 86-87 0.5238479364414736 1104 88-89 0.48154372135626744 1104 90-91 0.5531727578201036 1104 92-93 0.3550717749341352 1104 94-95 0.35841751406784184 1104 96-97 0.2781277660718757 1104 98-99 0.2904206080460554 1104 100-101 0.24189054679229471 1105 1 0.07818815321613926 1105 2 0.07983557107533912 1105 3 0.1875949919047386 1105 4 0.2013654943983525 1105 5 0.07717363668974286 1105 6 0.17067224441794338 1105 7 0.04999807087809671 1105 8 0.203070491299421 1105 9 0.21084029810214133 1105 10-11 0.1348537624608639 1105 12-13 0.1296192590350742 1105 14-15 0.2599091470281465 1105 16-17 0.2550872667022759 1105 18-19 0.1914139038249303 1105 20-21 0.1465950901972093 1105 22-23 0.19833632997185902 1105 24-25 0.08117543312770437 1105 26-27 0.18924551109216026 1105 28-29 0.18532521592663187 1105 30-31 0.24372467697267552 1105 32-33 0.09176145694193139 1105 34-35 0.3471372452619974 1105 36-37 0.20755585190497783 1105 38-39 0.1638393641677638 1105 40-41 0.16385350413019495 1105 42-43 0.20732310677437482 1105 44-45 0.13416682114537792 1105 46-47 0.01896194387690997 1105 48-49 0.08874601401511484 1105 50-51 0.11408059188993747 1105 52-53 0.1448471683437731 1105 54-55 0.1049852746365687 1105 56-57 0.08885138552929561 1105 58-59 0.16333530988302414 1105 60-61 0.21267414534774076 1105 62-63 0.02637774725588571 1105 64-65 -0.05163513483270066 1105 66-67 -0.16723105250289194 1105 68-69 0.012731893396143334 1105 70-71 0.08890520704295923 1105 72-73 0.12286624191370876 1105 74-75 -0.04337838703555974 1105 76-77 0.052139852322945046 1105 78-79 0.059528617473333156 1105 80-81 0.27940628114526334 1105 82-83 0.37126878468822966 1105 84-85 0.2733663892285172 1105 86-87 0.23950092654601818 1105 88-89 0.32355790495082815 1105 90-91 0.4019228729286688 1105 92-93 0.4166781579954808 1105 94-95 0.3862235835900911 1105 96-97 0.38804590862454447 1105 98-99 0.23559176200529208 1105 100-101 0.5767546853974359 1106 1 0.008508989200983308 1106 2 0.05691508591554495 1106 3 -0.032829707093213756 1106 4 0.01639101876240545 1106 5 0.06454291879398255 1106 6 0.037819803059200297 1106 7 0.05126190477857051 1106 8 0.07001164490668543 1106 9 0.06641774317617433 1106 10-11 0.03869791332325434 1106 12-13 -0.006245743181402474 1106 14-15 -0.06796016873598631 1106 16-17 -0.1081808299384619 1106 18-19 -0.030476906006803972 1106 20-21 -0.08107188512437347 1106 22-23 0.006894390755284974 1106 24-25 -0.0684155038495291 1106 26-27 -0.0704841046642315 1106 28-29 0.015812700160466875 1106 30-31 0.03590465708158064 1106 32-33 -0.08918399243040653 1106 34-35 -0.21757613118140995 1106 36-37 -0.031154427827829068 1106 38-39 -0.02917633404648967 1106 40-41 -0.09300422373778616 1106 42-43 -0.03956525795157262 1106 44-45 0.11321035927061018 1106 46-47 0.19244124685028652 1106 48-49 -0.09002519648372953 1106 50-51 0.17366654974266993 1106 52-53 0.19341205001970962 1106 54-55 0.10959668064225525 1106 56-57 -0.0027816529941375734 1106 58-59 -0.032794881521127195 1106 60-61 0.20516798812679582 1106 62-63 0.003849431660682967 1106 64-65 -0.03402306960108348 1106 66-67 -0.10312101385116534 1106 68-69 -0.11291574209129607 1106 70-71 0.061827089406062186 1106 72-73 0.14950455805508867 1106 74-75 0.2331084595654893 1106 76-77 0.20495737630085742 1106 78-79 0.11802285146932334 1106 80-81 0.138169984851789 1106 82-83 0.13792186037727205 1106 84-85 0.11933742647414647 1106 86-87 0.23637055604580937 1106 88-89 0.25882166518058014 1106 90-91 0.11952585633878599 1106 92-93 0.07710535925753703 1106 94-95 0.22098746258212287 1106 96-97 0.5345877669303469 1106 98-99 0.1638841128252615 1106 100-101 0.08138228625963606 1107 1 -0.11454775396835615 1107 2 -0.14873551756618753 1107 3 -0.09323355323810034 1107 4 -0.03026578049926343 1107 5 -0.01790675068522063 1107 6 -0.0056349813510436775 1107 7 0.017955918785808933 1107 8 0.0752931291731116 1107 9 0.023978118137563342 1107 10-11 0.012432469879378516 1107 12-13 -0.04083814900661764 1107 14-15 0.1598460900313441 1107 16-17 0.005027492970732794 1107 18-19 -0.0038858102449879084 1107 20-21 0.14376553330531294 1107 22-23 -0.054576910144199076 1107 24-25 -0.023611521853119655 1107 26-27 0.011012484372322717 1107 28-29 -0.12001902471130421 1107 30-31 -0.026224820230140722 1107 32-33 0.0038859492084171166 1107 34-35 -0.062141674184523765 1107 36-37 -0.11505905036696618 1107 38-39 -0.08848174420767663 1107 40-41 -0.12508482058999704 1107 42-43 -0.027280152375460887 1107 44-45 -0.002703812941227568 1107 46-47 -0.1793425870977714 1107 48-49 -0.12833604862593262 1107 50-51 0.01571876965255825 1107 52-53 -0.2279608517008498 1107 54-55 -0.3517659996021081 1107 56-57 -0.39165105243815646 1107 58-59 -0.20218614928699452 1107 60-61 -0.2560195200552684 1107 62-63 -0.3625887426178096 1107 64-65 -0.37368181485664564 1107 66-67 -0.2196567906484148 1107 68-69 -0.22314497523366583 1107 70-71 -0.23898282514630864 1107 72-73 -0.19708967310028314 1107 74-75 -0.2052715063411803 1107 76-77 -0.19244968117654082 1107 78-79 -0.330486229291985 1107 80-81 -0.2600149810059662 1107 82-83 -0.26828188616179816 1107 84-85 -0.02523869207633922 1107 86-87 -0.08175891381178602 1107 88-89 -0.1345824078493969 1107 90-91 -0.042435390569497855 1107 92-93 -0.12277353441907835 1107 94-95 -0.06388668607466741 1107 96-97 0.08738117896998077 1107 98-99 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-1.1089402965881767 2316 98-99 -1.4419115502516746 2316 100-101 -1.7503535796262106 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 10.0 5 30.0 6 100.0 7 250.0 8 673.0 9 1266.0 10 2175.0 11 3620.0 12 5469.0 13 7822.0 14 9097.0 15 11934.0 16 14586.0 17 18253.0 18 16980.0 19 13352.0 20 12692.0 21 12774.0 22 13669.0 23 14967.0 24 17611.0 25 21191.0 26 26184.0 27 32190.0 28 40709.0 29 50836.0 30 62924.0 31 77298.0 32 95869.0 33 120629.0 34 156109.0 35 210132.0 36 302214.0 37 381582.0 38 321818.0 39 65508.0 40 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.6008991267907 19.463942232481294 13.644181336511298 18.290977304216703 2 14.499246197146284 27.64090045601572 34.20892707949236 23.650926267345636 3 29.31022729076764 24.095025265424702 27.869336991551418 18.725410452256238 4 12.28959254054362 16.127648631152603 43.539041216408904 28.04371761189488 5 11.700813369371069 43.246635230884046 32.917072915738075 12.135478484006816 6 27.500456234402264 41.02091023780664 15.29540419100889 16.1832293367822 7 28.726496089507503 32.697803431301395 21.98500958428877 16.590690894902334 8 23.895053088138585 29.68917220402237 18.90520211951167 27.510572588327374 9 21.2655461737755 25.47647240298912 23.53193759454188 29.7260438286935 10-11 26.30679385082115 24.67769318281264 27.948379568857384 21.067133397508826 12-13 32.359424597294186 22.901222784423744 24.89310729316069 19.846245325121384 14-15 22.57166407778591 27.7588382895464 30.04479354920616 19.624704083461534 16-17 19.29289091739869 28.57487903540096 30.141783065096156 21.990446982104196 18-19 18.62096389894561 35.5298942531139 27.143909951091423 18.70523189684906 20-21 23.026845838082888 26.674410658421344 26.182277415084492 24.116466088411272 22-23 19.791217925770418 29.004153425163903 26.700166949115754 24.50446169994992 24-25 25.31015337195675 28.960304195570828 26.06774857659193 19.66179385588049 26-27 24.555421930534337 24.05255049660509 32.8539791770571 18.538048395803486 28-29 19.792168207220318 34.435965986069085 27.541248367744416 18.230617438966185 30-31 22.761822609397033 26.958672154080766 27.05291415016265 23.22659108635956 32-33 22.810081761801833 29.27370763189499 28.07803988250796 19.83817072379522 34-35 25.66661096599894 25.857844738900788 29.10492138737605 19.37062290772422 36-37 23.910388676743814 24.722240376445985 32.3430774832644 19.0242934635458 38-39 21.03806548176826 29.72392332336673 30.803663222350146 18.434347972514864 40-41 20.60585168368765 30.172106558982108 28.02150392579213 21.20053783153811 42-43 19.30577264956228 31.382186524681355 25.01364016748119 24.29840065827518 44-45 23.10912740733204 26.031063643691287 25.83573737881954 25.024071570157137 46-47 25.605453645090208 30.14507831026988 26.334018959500447 17.91544908513946 48-49 24.891800450767278 28.980653598553886 26.61930607602369 19.508239874655146 50-51 18.69181342584533 30.082555100909236 32.433377874878246 18.792253598367196 52-53 23.078789994627943 31.90576359501141 25.204742531045675 19.81070387931497 54-55 19.563547311661843 29.52658700839973 26.681777809722814 24.228087870215614 56-57 24.245211225106317 26.025110890759624 28.126521998575054 21.60315588555901 58-59 29.143354411695725 25.59741020148556 25.193360934265336 20.06587445255338 60-61 22.05766347584667 29.063417780839767 31.15754510370632 17.721373639607243 62-63 20.031359281061174 27.652859573858272 30.66415344295078 21.651627702129776 64-65 19.202009568283227 35.16609079993061 26.168864719529665 19.463034912256493 66-67 22.57075104497568 26.98554715829873 25.34971992761091 25.09398186911468 68-69 20.10528669130108 29.79448138673373 25.691903641067505 24.40832828089769 70-71 25.207385821990425 29.380235694187967 24.81610238571581 20.596276098105797 72-73 24.300991006590685 24.715446737071247 31.175238697096802 19.808323559241266 74-75 20.04367187241111 34.57827962600037 26.255210450631928 19.122838050956588 76-77 22.680296410524 27.4441103180341 25.979910098577925 23.895683172863976 78-79 22.676451184641238 29.74406652576102 26.638284843635102 20.941197445962636 80-81 25.0919224033963 26.978350278730943 26.98857165793571 20.941155659937046 82-83 23.184841741312294 25.580378312380287 30.82753966268515 20.40724028362227 84-85 20.680888700122633 29.52212707417619 29.002633522631143 20.79435070307003 86-87 20.78208118587526 29.664360616600995 26.45741469578925 23.0961435017345 88-89 19.64615842010222 30.625128058886318 24.47790479559818 25.250808725413275 90-91 22.632760018463816 25.237530273237407 25.73270685798217 26.397002850316603 92-93 24.139199250992615 29.391865559522234 26.449183200167646 20.0197519893175 94-95 23.154436262915894 28.96140534457905 26.6765955828147 21.207562809690355 96-97 17.70123536825932 30.25901964449529 31.702946872653946 20.33679811459145 98-99 21.931697017091498 31.791483678493428 24.98386857009372 21.29295073432136 100-101 18.054793868396327 29.552390878299068 26.14150780919274 26.25130744411187 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 228.0 1 167.0 2 219.0 3 310.5 4 243.0 5 225.0 6 330.0 7 574.5 8 886.0 9 1107.0 10 1156.5 11 1167.5 12 1304.0 13 1432.5 14 1581.5 15 1829.5 16 2084.5 17 2330.0 18 2571.5 19 2867.0 20 3148.0 21 3535.5 22 4255.0 23 5250.5 24 6487.5 25 8097.0 26 10230.5 27 13077.0 28 16857.0 29 21224.5 30 25718.0 31 29888.5 32 34087.0 33 38078.5 34 41373.0 35 44937.5 36 48551.0 37 51770.5 38 54636.0 39 56414.5 40 57347.0 41 58104.5 42 59394.0 43 61446.5 44 62315.0 45 63755.5 46 73241.5 47 133557.0 48 282323.5 49 281685.5 50 136924.5 51 72982.0 52 49938.0 53 39566.0 54 33947.5 55 29735.5 56 25047.5 57 19848.5 58 15625.0 59 12511.0 60 9840.0 61 7430.0 62 5471.0 63 3845.0 64 2541.0 65 1658.5 66 1000.5 67 573.5 68 320.0 69 201.5 70 113.0 71 58.0 72 35.5 73 21.0 74 11.0 75 8.5 76 9.0 77 9.5 78 5.0 79 2.5 80 2.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05404726755591069 2 0.0046206213195467685 3 0.04233235895786787 4 0.0 5 0.0 6 0.001400188278650536 7 0.0 8 0.0 9 0.0 10-11 3.0337412704094946E-4 12-13 0.0 14-15 0.0034071248113829705 16-17 0.0 18-19 0.0 20-21 0.012274983909503032 22-23 0.0 24-25 0.0 26-27 0.008051082602240581 28-29 0.009871327364486277 30-31 0.00989466383579712 32-33 0.005880790770332251 34-35 0.007701035532577947 36-37 0.02002269238470266 38-39 0.011551553298866922 40-41 0.012251647438192188 42-43 0.0 44-45 0.0 46-47 9.334588524336905E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0015168706352047471 58-59 3.967200122843185E-4 60-61 0.008074419073551425 62-63 0.014025219257816203 64-65 0.047349700289698954 66-67 0.0249000148886687 68-69 0.0017735718196240122 70-71 2.1002824179758037E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.3336471310842264E-5 80-81 4.667294262168453E-5 82-83 4.667294262168453E-5 84-85 7.000941393252679E-5 86-87 7.701035532577948E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 2.5670118441926493E-4 96-97 4.4339295490600304E-4 98-99 5.834117827710566E-4 100-101 0.001400188278650536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2142569.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.18897672271796 #Duplication Level Percentage of deduplicated Percentage of total 1 82.93951914144483 38.30891519017616 2 10.772808255995516 9.951699795489615 3 2.656223324028214 3.6806471185163914 4 1.041318060115049 1.9238966255839924 5 0.586987023509757 1.3556164982714833 6 0.3513281010320315 0.9736491288363123 7 0.25884948842862937 0.8369195097002189 8 0.1897539678211936 0.7011633282189188 9 0.1352850333224064 0.5623809529554677 >10 0.9294504588880289 8.236700274396553 >50 0.0847932646534777 2.6975965195745393 >100 0.045025548226738106 3.8510088918628194 >500 0.003259607542296343 1.009538016245117 >1k 0.0029540193352060613 3.327142207987589 >5k 8.149018855740858E-4 2.077464282392839 >10k+ 0.0016298037711481716 20.505661659792054 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 141994 6.627277814623474 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 39054 1.8227651011472674 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 37454 1.7480883929525723 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 26380 1.2312322263600377 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 22054 1.0293250765786306 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 21118 0.9856392022847339 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 19910 0.929258287597739 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 19573 0.9135295059342312 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 17554 0.8192968347810503 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 17465 0.8151429428877203 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 15190 0.708961998423388 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 13700 0.6394193139170781 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 12742 0.5947066348855043 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 10917 0.50952851460093 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10553 0.49253956348663686 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 10175 0.47489719117564005 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 6071 0.28335143465624674 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5972 0.2787308133367 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 5938 0.27714393328756276 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5462 0.2549276125996409 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 5391 0.2516138336735013 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5155 0.24059901921478374 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5141 0.23994559801808016 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5025 0.23453153667396476 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4558 0.21273527246963808 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4219 0.19691314492088705 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4072 0.1900522223554994 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3990 0.1862250410605213 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3850 0.17969082909348544 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3457 0.1613483626431634 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3395 0.15845464020061897 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3034 0.14160570791419086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2951 0.13773185367659105 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2880 0.13441807475045145 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2563 0.11962275193937745 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2497 0.11654233772634627 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2454 0.11453540119361384 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 2426 0.11322855880020667 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2380 0.11108160343960918 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 2283 0.10655432800530577 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.667294262168453E-5 0.0 10-11 0.0 0.0 0.0 7.000941393252679E-5 0.0 12-13 0.0 2.3336471310842264E-5 0.0 2.1002824179758037E-4 0.0 14-15 0.0 4.667294262168453E-5 0.0 3.267105983517917E-4 0.0 16-17 0.0 4.667294262168453E-5 0.0 3.733835409734762E-4 0.0 18-19 0.0 4.667294262168453E-5 0.0 6.067482540818989E-4 0.0 20-21 0.0 4.667294262168453E-5 0.0 0.001050141208987902 0.0 22-23 0.0 4.667294262168453E-5 0.0 0.0021702918319083304 0.0 24-25 0.0 4.667294262168453E-5 0.0 0.0036404895244913933 0.0 26-27 0.0 4.667294262168453E-5 0.0 0.006020809598197304 0.0 28-29 0.0 4.667294262168453E-5 0.0 0.013138433348004196 0.0 30-31 4.667294262168453E-5 4.667294262168453E-5 0.0 0.025063370187844592 0.0 32-33 4.667294262168453E-5 4.667294262168453E-5 0.0 0.046859634392171265 0.0 34-35 4.667294262168453E-5 4.667294262168453E-5 0.0 0.06874924448174131 0.0 36-37 4.667294262168453E-5 9.334588524336905E-5 0.0 0.09423267115318107 0.0 38-39 4.667294262168453E-5 1.4001882786505358E-4 0.0 0.13033419227105406 0.0 40-41 4.667294262168453E-5 1.4001882786505358E-4 0.0 0.17691378900749521 0.0 42-43 4.667294262168453E-5 1.4001882786505358E-4 0.0 0.23922216740744406 0.0 44-45 4.667294262168453E-5 1.4001882786505358E-4 0.0 0.28470494999227564 0.0 46-47 4.667294262168453E-5 1.4001882786505358E-4 0.0 0.3807345294363915 0.0 48-49 4.667294262168453E-5 1.4001882786505358E-4 0.0 0.4861453703474661 0.0 50-51 4.667294262168453E-5 1.4001882786505358E-4 0.0 0.5246972209529774 0.0 52-53 4.667294262168453E-5 1.4001882786505358E-4 0.0 0.5879623946766708 0.0 54-55 4.667294262168453E-5 1.4001882786505358E-4 0.0 0.7212136458615802 0.0 56-57 4.667294262168453E-5 1.4001882786505358E-4 0.0 0.9235408521265827 0.0 58-59 4.667294262168453E-5 1.4001882786505358E-4 0.0 1.1584924452841425 0.0 60-61 4.667294262168453E-5 1.866917704867381E-4 0.0 1.4006083351341312 0.0 62-63 4.667294262168453E-5 1.866917704867381E-4 0.0 1.621651391390429 0.0 64-65 4.667294262168453E-5 1.866917704867381E-4 0.0 1.8445146924089726 0.0 66-67 4.667294262168453E-5 1.866917704867381E-4 0.0 2.357100284751623 0.0 68-69 4.667294262168453E-5 1.866917704867381E-4 0.0 2.8957527155484843 0.0 70-71 4.667294262168453E-5 1.866917704867381E-4 0.0 3.0412089412289642 0.0 72-73 4.667294262168453E-5 1.866917704867381E-4 0.0 3.230677751801692 0.0 74-75 4.667294262168453E-5 1.866917704867381E-4 0.0 3.449947236238366 0.0 76-77 4.667294262168453E-5 1.866917704867381E-4 0.0 3.6240839851598707 0.0 78-79 4.667294262168453E-5 1.866917704867381E-4 0.0 3.8469939591210363 0.0 80-81 4.667294262168453E-5 1.866917704867381E-4 0.0 4.25962944483935 0.0 82-83 4.667294262168453E-5 1.866917704867381E-4 0.0 4.768831248841927 0.0 84-85 4.667294262168453E-5 1.866917704867381E-4 0.0 5.791155384027306 0.0 86-87 4.667294262168453E-5 1.866917704867381E-4 0.0 7.119257302798649 0.0 88-89 4.667294262168453E-5 1.866917704867381E-4 0.0 8.469785570499713 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2025 0.0 47.86859 2 TATAACG 230 0.0 45.450676 2 ACCTGGG 2155 0.0 42.553493 3 AGTACGC 1715 0.0 40.728523 2 GAGTACG 1815 0.0 39.560364 1 TACGCAG 1790 0.0 39.264557 4 GTACGCA 1780 0.0 39.239418 3 GTACCTG 2710 0.0 37.02313 1 ATAACGC 310 0.0 36.785336 3 CGCAGTG 1965 0.0 36.01023 6 ACGCAGT 1980 0.0 35.737427 5 GTACCGG 135 4.1884596E-7 28.178392 1 GTATAAC 595 0.0 27.172018 1 TAACGCA 445 0.0 26.679153 4 TCACGCA 275 0.0 25.903032 4 CCTGGGG 1490 0.0 25.178677 4 TATCACG 305 9.094947E-12 21.810905 2 GTACACG 240 5.862603E-9 21.794226 1 GTAGGCA 1205 0.0 20.898449 3 CTGGGGG 1090 0.0 20.04165 5 >>END_MODULE