##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003808_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2540877 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.845358512041315 31.0 31.0 34.0 28.0 34.0 2 30.718462168770863 31.0 31.0 34.0 27.0 34.0 3 30.812413981471753 31.0 31.0 34.0 27.0 34.0 4 34.22981789358556 37.0 35.0 37.0 30.0 37.0 5 34.19375869040493 37.0 35.0 37.0 30.0 37.0 6 34.338877875631134 37.0 35.0 37.0 30.0 37.0 7 34.400013853484445 37.0 35.0 37.0 30.0 37.0 8 34.373775275229775 37.0 35.0 37.0 30.0 37.0 9 35.82598134423665 39.0 35.0 39.0 30.0 39.0 10-11 35.64399083466063 39.0 35.0 39.0 30.0 39.0 12-13 35.501841293380195 39.0 35.0 39.0 30.0 39.0 14-15 36.44755137694583 39.0 36.0 41.0 29.5 41.0 16-17 36.57282091970607 39.0 36.0 41.0 30.0 41.0 18-19 36.591986743159936 39.0 36.0 41.0 30.0 41.0 20-21 36.46488279440524 39.0 36.0 41.0 29.5 41.0 22-23 36.38479391170844 39.0 36.0 41.0 29.5 41.0 24-25 36.156432208249356 39.0 36.0 41.0 28.0 41.0 26-27 35.94615796042075 39.0 35.5 41.0 27.0 41.0 28-29 35.79106288891592 39.0 35.0 40.0 27.0 41.0 30-31 35.599629576717014 38.5 35.0 40.0 27.0 41.0 32-33 35.36277001995768 38.0 35.0 40.0 26.5 41.0 34-35 35.29718105205407 38.0 35.0 40.0 26.0 41.0 36-37 35.14986813608057 38.0 34.5 40.0 25.0 41.0 38-39 35.16434109167818 38.0 34.5 40.0 25.5 41.0 40-41 35.31655861342364 39.0 35.0 40.0 25.5 41.0 42-43 35.15965511120766 39.0 35.0 40.0 25.0 41.0 44-45 34.92227368739219 38.0 34.5 40.0 24.0 41.0 46-47 34.624347420201765 38.0 34.0 40.0 23.5 41.0 48-49 34.33666486020378 38.0 34.0 40.0 22.5 41.0 50-51 33.54905432258232 37.5 32.5 39.5 20.5 40.5 52-53 33.72175650375835 38.0 33.0 39.5 21.5 40.5 54-55 34.06334269624229 38.0 34.0 40.0 21.5 41.0 56-57 33.783529663183224 38.0 33.0 40.0 19.0 41.0 58-59 33.492684612438936 38.0 33.0 40.0 18.0 41.0 60-61 33.136349378580704 37.5 32.0 40.0 13.5 41.0 62-63 32.92981498120531 37.0 32.0 40.0 13.0 41.0 64-65 32.64325486829941 36.5 32.0 39.5 12.0 41.0 66-67 32.20161621361443 36.0 31.0 39.0 8.0 41.0 68-69 31.782414890606667 36.0 31.0 39.0 7.5 40.5 70-71 31.294922776663334 35.0 30.5 38.5 6.5 40.0 72-73 30.90318106700954 35.0 30.0 37.5 2.0 40.0 74-75 30.55385305939642 35.0 30.0 37.0 2.0 39.0 76-77 30.119635464447903 34.5 29.5 36.5 2.0 39.0 78-79 29.518833457896626 34.0 29.0 36.0 2.0 39.0 80-81 29.007989564233135 34.0 28.5 36.0 2.0 37.5 82-83 28.625973236799737 34.0 27.5 35.0 2.0 37.0 84-85 28.351971779822478 34.0 27.0 35.0 2.0 37.0 86-87 28.21704769652368 34.0 27.0 35.0 2.0 36.0 88-89 27.8355603203146 34.0 26.5 35.0 2.0 36.0 90-91 27.402514564853 34.0 25.5 35.0 2.0 35.5 92-93 27.09715739880364 33.0 25.0 35.0 2.0 35.0 94-95 26.836884075852552 33.0 25.0 35.0 2.0 35.0 96-97 26.547377736112374 33.0 25.0 35.0 2.0 35.0 98-99 26.299840370076943 33.0 24.5 35.0 2.0 35.0 100-101 25.982863003600727 33.0 23.5 35.0 2.0 35.0 102-103 25.517106298337147 32.0 19.5 35.0 2.0 35.0 104-105 25.136003828599335 32.0 19.0 35.0 2.0 35.0 106-107 24.70544087730339 32.0 17.5 35.0 2.0 35.0 108-109 24.402936269642332 32.0 12.5 34.0 2.0 35.0 110-111 24.03003844735499 31.5 6.0 34.0 2.0 35.0 112-113 23.42184647269427 31.0 2.0 34.0 2.0 35.0 114-115 22.855803921244515 31.0 2.0 34.0 2.0 35.0 116-117 22.1704383958767 31.0 2.0 34.0 2.0 35.0 118-119 21.42513962698706 30.0 2.0 34.0 2.0 35.0 120-121 19.79802997154132 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6191034216969094 1101 2 0.5558243339695323 1101 3 0.6582448599671196 1101 4 0.8796134624478071 1101 5 0.8138853578781635 1101 6 0.8518898397689938 1101 7 0.7154635312436923 1101 8 0.7202137152341663 1101 9 0.8509192546930961 1101 10-11 0.8922178416699253 1101 12-13 1.0321711988987587 1101 14-15 1.0875548719909887 1101 16-17 1.0282939326749982 1101 18-19 0.8636058804941342 1101 20-21 1.0450624983219683 1101 22-23 0.9302105076025171 1101 24-25 0.9795591359907192 1101 26-27 1.019426393131802 1101 28-29 0.9874055020388823 1101 30-31 1.0376377410541053 1101 32-33 0.952055507609785 1101 34-35 0.9644993886234872 1101 36-37 0.9626231261846812 1101 38-39 0.9611730974257711 1101 40-41 0.9813824869128922 1101 42-43 0.8280853628962817 1101 44-45 0.9202614695664053 1101 46-47 0.8610509346456467 1101 48-49 1.0164412749651177 1101 50-51 0.7780097646508608 1101 52-53 0.8246470325293203 1101 54-55 0.9623157407969956 1101 56-57 0.9657762008622583 1101 58-59 1.0016145126499012 1101 60-61 0.9565979375542781 1101 62-63 0.8283132528396138 1101 64-65 0.9381606694084681 1101 66-67 0.9364061417188765 1101 68-69 0.9426216685022908 1101 70-71 0.6306259982571838 1101 72-73 0.5642768828234317 1101 74-75 0.5968039236740488 1101 76-77 0.5456142167829476 1101 78-79 0.7212625368399124 1101 80-81 0.808329027004735 1101 82-83 0.9385596986212086 1101 84-85 0.6062492577707843 1101 86-87 0.6657599795244984 1101 88-89 0.644078367815478 1101 90-91 0.571376487787429 1101 92-93 0.6353080537189157 1101 94-95 0.5922215575095535 1101 96-97 0.5425046948470964 1101 98-99 0.5128415285679786 1101 100-101 0.5788977353675513 1101 102-103 0.2858992218308991 1101 104-105 0.23439593808088333 1101 106-107 0.13014426387310252 1101 108-109 0.20144953776621932 1101 110-111 0.2778181970849971 1101 112-113 0.37734947321229484 1101 114-115 0.3298687458420595 1101 116-117 0.13415183754029414 1101 118-119 0.2086508640096092 1101 120-121 0.44454852741119666 1102 1 0.464584413575448 1102 2 0.5585795794432258 1102 3 0.6766820237798576 1102 4 0.8319580387065386 1102 5 0.8678940679931557 1102 6 0.9047641310183465 1102 7 0.7075468089682602 1102 8 0.8117780679124706 1102 9 0.8489877563385022 1102 10-11 0.9736706769551517 1102 12-13 1.0366707777468278 1102 14-15 1.161146225091798 1102 16-17 0.9154322206819643 1102 18-19 0.9446232468973008 1102 20-21 1.0330559815888307 1102 22-23 0.9027512052326401 1102 24-25 1.1032417612062986 1102 26-27 1.0511630850626617 1102 28-29 1.1196784222525338 1102 30-31 1.1482554395614315 1102 32-33 0.8724258411124595 1102 34-35 0.9667417430630891 1102 36-37 1.081413900315269 1102 38-39 1.1230733860506419 1102 40-41 1.0932670207442854 1102 42-43 1.2938111906558305 1102 44-45 1.2355826621231643 1102 46-47 1.1594880148916147 1102 48-49 1.0076770873557663 1102 50-51 0.6765200149751109 1102 52-53 0.7561754957389226 1102 54-55 0.8316962136521724 1102 56-57 0.9878844650502359 1102 58-59 0.9580300193975475 1102 60-61 1.244076465196322 1102 62-63 1.2130996893633963 1102 64-65 0.988237501677439 1102 66-67 1.0076890243201078 1102 68-69 0.8302294665597572 1102 70-71 0.6726726786533774 1102 72-73 0.6831911637203412 1102 74-75 0.782860230961834 1102 76-77 0.6676033245921893 1102 78-79 0.7997903509323656 1102 80-81 0.9670783569344401 1102 82-83 0.8581054247586515 1102 84-85 0.8752076078481963 1102 86-87 0.9451692755408452 1102 88-89 0.8730430763407391 1102 90-91 0.9907273067294575 1102 92-93 0.9372291308060632 1102 94-95 0.642438364113687 1102 96-97 0.72121032181537 1102 98-99 0.8467774028717621 1102 100-101 0.743107170399675 1102 102-103 0.5502313525401377 1102 104-105 0.7117133367064064 1102 106-107 0.6106320996240946 1102 108-109 0.6155960519382795 1102 110-111 0.4030865631556111 1102 112-113 0.2966497219708977 1102 114-115 0.37290704424571075 1102 116-117 0.568262390966126 1102 118-119 0.3848291492834477 1102 120-121 0.5669059434579324 1103 1 0.7402768543200899 1103 2 0.6887798573618049 1103 3 0.7484866370549668 1103 4 0.8852402072945864 1103 5 0.9672622797831423 1103 6 0.9188734268857459 1103 7 0.8582257112007028 1103 8 0.8148541532679161 1103 9 0.7543901100900783 1103 10-11 0.942469686369293 1103 12-13 1.0290581400330794 1103 14-15 1.1867383454610803 1103 16-17 1.0537873537276283 1103 18-19 0.9343137375546391 1103 20-21 0.9655279812379121 1103 22-23 1.0119749800514413 1103 24-25 1.005835550603372 1103 26-27 1.1117486040534104 1103 28-29 0.9703128233954033 1103 30-31 0.9965518750336386 1103 32-33 0.873634424095961 1103 34-35 0.914621991131952 1103 36-37 0.8070454583705668 1103 38-39 0.7651525576453224 1103 40-41 0.8359816765628167 1103 42-43 0.7287450333621521 1103 44-45 0.6878344957792564 1103 46-47 0.7799137577768533 1103 48-49 0.966370477420881 1103 50-51 0.64613370436048 1103 52-53 0.7049681718370593 1103 54-55 0.6636405633270286 1103 56-57 0.7041965174670253 1103 58-59 0.9517241188807049 1103 60-61 0.6706844613665481 1103 62-63 0.7756338064141275 1103 64-65 0.88539449534791 1103 66-67 0.8268926863251949 1103 68-69 0.6662612284509066 1103 70-71 0.6477741362637524 1103 72-73 0.46872657667745443 1103 74-75 0.49430755748475264 1103 76-77 0.6512643034591115 1103 78-79 0.9108246986793382 1103 80-81 0.7957423251048574 1103 82-83 0.8102147655739103 1103 84-85 0.9585556981598202 1103 86-87 0.8614393991853682 1103 88-89 0.9921782605511531 1103 90-91 1.0545062899763629 1103 92-93 0.9723159451616503 1103 94-95 1.0421625981588214 1103 96-97 0.8995354751796363 1103 98-99 0.8612457400825271 1103 100-101 0.8364601535296039 1103 102-103 0.8889555860437319 1103 104-105 0.83981324984331 1103 106-107 0.7680464793652817 1103 108-109 0.7981991043853931 1103 110-111 0.9674156161529304 1103 112-113 0.8673784973745633 1103 114-115 0.8159475449757103 1103 116-117 0.6647672074272251 1103 118-119 0.6208846848020073 1103 120-121 0.6718530376599823 1104 1 0.5073215103938402 1104 2 0.7229892064221204 1104 3 0.6667660923567311 1104 4 0.8284188315895875 1104 5 1.0084747580008369 1104 6 0.8920366864559028 1104 7 0.7448950557640259 1104 8 0.8552105633561666 1104 9 0.8877388237628097 1104 10-11 0.9650290138815194 1104 12-13 1.0354256590984576 1104 14-15 1.1050289261352546 1104 16-17 1.0083217627478405 1104 18-19 0.8154868553758448 1104 20-21 0.9334772471975512 1104 22-23 0.9419551066230483 1104 24-25 1.1062104170989002 1104 26-27 1.026664683612701 1104 28-29 0.9440238611701375 1104 30-31 0.925962265428808 1104 32-33 0.9957318729895164 1104 34-35 0.9731309101673489 1104 36-37 0.8558135283175403 1104 38-39 0.8834799691576265 1104 40-41 1.0129758117349112 1104 42-43 1.0601051745489798 1104 44-45 1.0510439432112904 1104 46-47 1.205845127111111 1104 48-49 1.182703636651894 1104 50-51 0.8920843615772185 1104 52-53 1.0393251370228427 1104 54-55 1.0582238537203281 1104 56-57 1.1502147466824866 1104 58-59 1.1381142165966054 1104 60-61 0.9363993426646786 1104 62-63 1.155178813815425 1104 64-65 1.013232636403579 1104 66-67 0.8799416824652724 1104 68-69 0.874820680098864 1104 70-71 0.7123452121692431 1104 72-73 0.693353993810998 1104 74-75 0.7513089300043809 1104 76-77 0.6966924195002591 1104 78-79 0.7536553915909572 1104 80-81 0.7615268278124212 1104 82-83 0.7646426052373876 1104 84-85 0.9327079447823898 1104 86-87 1.034303679010737 1104 88-89 0.877568480060603 1104 90-91 0.9130506930942737 1104 92-93 0.9885349814936042 1104 94-95 0.9779314316576695 1104 96-97 0.9206769032558597 1104 98-99 0.9645594615313051 1104 100-101 0.959155737576527 1104 102-103 0.9204667395223431 1104 104-105 0.8706955270079568 1104 106-107 0.9581495237356101 1104 108-109 0.8492467045840115 1104 110-111 0.8325198901696815 1104 112-113 0.7597238312762649 1104 114-115 0.7320891721375453 1104 116-117 0.5599644427136248 1104 118-119 0.5225068226535683 1104 120-121 0.6042731340642611 1105 1 0.8187850898285767 1105 2 0.974819179637155 1105 3 0.9583323213503512 1105 4 1.1574537176145085 1105 5 1.1237007053460744 1105 6 1.036691168981534 1105 7 1.0470947298969335 1105 8 1.0007235474348661 1105 9 1.0755521643566368 1105 10-11 1.3004075100747485 1105 12-13 1.3215258454100436 1105 14-15 1.5269120558848073 1105 16-17 1.4296036919494455 1105 18-19 1.3076705355072207 1105 20-21 1.2776654518352402 1105 22-23 1.2826408815124566 1105 24-25 1.4022598131899215 1105 26-27 1.6006339115528831 1105 28-29 1.5584985751098657 1105 30-31 1.6017646026627617 1105 32-33 1.4375579688708413 1105 34-35 1.388836677558686 1105 36-37 1.4496271853702325 1105 38-39 1.1482778061185925 1105 40-41 1.2863739882476821 1105 42-43 1.4076897880914245 1105 44-45 1.2559175521109083 1105 46-47 1.4755122608634466 1105 48-49 1.5054827583502828 1105 50-51 1.1163681595966466 1105 52-53 1.1607860008984972 1105 54-55 1.1399267230898218 1105 56-57 1.215982950550412 1105 58-59 1.239982963148698 1105 60-61 1.190197205284477 1105 62-63 1.2148275658699177 1105 64-65 1.2277958376557265 1105 66-67 1.0592129504246728 1105 68-69 1.1052843350845905 1105 70-71 0.8722100329209574 1105 72-73 1.0227103279060152 1105 74-75 0.874351909963412 1105 76-77 0.9862927191383299 1105 78-79 0.9818304218469898 1105 80-81 1.0989150871889422 1105 82-83 1.1980399549657612 1105 84-85 1.1897507579590538 1105 86-87 1.1431746122650033 1105 88-89 1.0410903962617297 1105 90-91 1.1797164569503984 1105 92-93 1.123504682696577 1105 94-95 1.2112243285818884 1105 96-97 1.202932898511829 1105 98-99 1.2211910207468897 1105 100-101 1.0411305192270426 1105 102-103 1.0937869417138906 1105 104-105 1.3497290588170614 1105 106-107 1.0186081324855536 1105 108-109 1.1850414439544856 1105 110-111 1.267183431686373 1105 112-113 1.2395260587474617 1105 114-115 1.1569350053580258 1105 116-117 1.1571835961719366 1105 118-119 1.351040470640136 1105 120-121 1.2330542471726424 1106 1 0.5617115893424511 1106 2 0.717239803906061 1106 3 0.660619128824198 1106 4 1.066302359920762 1106 5 1.0324007720479926 1106 6 1.0034497340733566 1106 7 0.8649028851265754 1106 8 0.9418023230282486 1106 9 0.9437781716408864 1106 10-11 1.0965137519558397 1106 12-13 1.0845396768561741 1106 14-15 1.1487027555008495 1106 16-17 0.9746829217981485 1106 18-19 0.9185416015080037 1106 20-21 1.0471602768257497 1106 22-23 0.934244555414601 1106 24-25 1.1586781895233926 1106 26-27 1.088905640622798 1106 28-29 1.0042155129980799 1106 30-31 0.9260230039174431 1106 32-33 0.922461834619547 1106 34-35 1.003935415249579 1106 36-37 1.0682268760097884 1106 38-39 1.1456012008960954 1106 40-41 1.090538100102222 1106 42-43 0.9628559997546517 1106 44-45 1.0164412833605283 1106 46-47 1.0746191464225205 1106 48-49 1.0036008755068622 1106 50-51 0.7005052674984427 1106 52-53 0.9363775876590168 1106 54-55 1.1650460997725176 1106 56-57 1.042418590381601 1106 58-59 1.0169247475461916 1106 60-61 1.0342698017486427 1106 62-63 0.8861318711510364 1106 64-65 0.8006504014404427 1106 66-67 0.9324229734875544 1106 68-69 0.807531193226751 1106 70-71 0.6707159279161594 1106 72-73 0.7988908306571325 1106 74-75 0.6796337053671735 1106 76-77 0.9184535919549184 1106 78-79 0.9669145084571404 1106 80-81 0.7521670076745117 1106 82-83 0.8369280708026601 1106 84-85 0.8477983937216962 1106 86-87 0.7111412182146672 1106 88-89 0.637672394284909 1106 90-91 0.6181704716786847 1106 92-93 0.7460277119152501 1106 94-95 0.7163652240394249 1106 96-97 0.9283337608787505 1106 98-99 0.7464589730339206 1106 100-101 0.8040797135522624 1106 102-103 0.6210090447364855 1106 104-105 0.5209355106790134 1106 106-107 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-0.1426301217388506 2316 116-117 -0.3702242490289258 2316 118-119 -0.27505604147619067 2316 120-121 -0.5342330828014177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39814.0 3 5784.0 4 5066.0 5 5517.0 6 6350.0 7 6531.0 8 8127.0 9 9632.0 10 12405.0 11 15308.0 12 17056.0 13 20005.0 14 25141.0 15 25676.0 16 19748.0 17 19479.0 18 20848.0 19 22653.0 20 24694.0 21 27538.0 22 30895.0 23 34141.0 24 38088.0 25 42831.0 26 48451.0 27 56467.0 28 65937.0 29 76336.0 30 90506.0 31 107853.0 32 129481.0 33 159951.0 34 201834.0 35 259342.0 36 342017.0 37 379441.0 38 139126.0 39 808.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.98348127159012 18.564804470378405 14.002954151334016 21.44876010669746 2 13.604925527794764 21.692052719434347 38.612222046381206 26.090799706389685 3 22.734490354029564 24.339269019440668 30.991176998079673 21.935063628450095 4 10.947871971602963 15.599842874818304 42.781743898676524 30.67054125490221 5 10.809270871727707 39.05301634144024 36.420151434364065 13.717561352467994 6 27.129229031796275 37.25639822488031 18.299082218581493 17.315290524741926 7 24.608677918721085 33.5226123042197 23.669504923651697 18.19920485340752 8 22.5163851899903 30.994113693284824 21.217295701354498 25.27220541537038 9 21.75480310604037 19.929630760229454 26.050132916141997 32.265433217588175 10-11 24.146933276100537 24.384548735314223 28.502666889238643 22.965851099346594 12-13 27.7811671804427 22.972823659028656 27.721012459400036 21.52499670112861 14-15 22.70706144927663 25.070164975877717 30.298034466538514 21.92473910830714 16-17 19.473420056430637 28.996920067859875 30.165644494931875 21.364015380777605 18-19 18.474472578160153 31.45054004234605 29.934667630842192 20.14031974865161 20-21 20.660891015785985 27.478569938928025 27.894000099801847 23.966538945484135 22-23 19.2341305483776 27.21632493729656 29.487104825208505 24.062439689117337 24-25 22.58956491391807 26.787651328543078 28.57763789743306 22.045145860105787 26-27 21.452639252534077 24.77546966043103 32.82068139567145 20.95120969136345 28-29 18.948278024725422 29.726046812950763 30.446746079091852 20.87892908323197 30-31 19.826946110760453 27.221128279550122 29.402342837547028 23.5495827721424 32-33 20.58570951479779 27.086957904613477 30.510388670663634 21.816943909925097 34-35 22.969282122955008 25.876595685461346 29.63610120935115 21.5180209822325 36-37 21.72158893327313 25.24793903565777 32.47049495154947 20.55997707951963 38-39 21.12344513473362 27.49434969451871 31.27640147356229 20.105803697185372 40-41 20.081464564922243 28.935566928837993 30.56112059105548 20.421847915184284 42-43 19.21483894399792 28.975588286337423 27.820495311342334 23.989077458322324 44-45 20.318920931245778 26.923351366972938 28.045161814796728 24.712565886984553 46-47 22.52888768667759 28.100183067807833 28.71878968150256 20.65213956401202 48-49 21.93988876623219 27.19934345761235 29.179981896984543 21.68078587917092 50-51 18.6252292460848 28.326078636998453 32.105068015884456 20.94362410103229 52-53 20.077279322613347 29.714217566734906 28.122219792888238 22.08628331776351 54-55 18.98394398434653 27.4088347148198 29.69590599331949 23.911315307514176 56-57 21.748120887686035 25.970204288391557 28.691404342726855 23.590270481195553 58-59 23.945652588820217 25.882774651605907 28.185030584882192 21.986542174691685 60-61 21.735605862222616 26.496101733704997 31.86608757861701 19.902204825455378 62-63 20.098408910830866 27.816250888057624 30.72140692977159 21.36393327133992 64-65 18.985221612214502 30.720677459708952 28.981969945536573 21.31213098253998 66-67 20.01006927446167 27.613285797453347 27.439687212478585 24.9369577156064 68-69 19.248838984668236 28.27436144156753 28.849680323175775 23.62711925058846 70-71 22.018963630345386 27.280053994360014 27.669544974260234 23.031437401034363 72-73 20.978701907219786 25.527352801365122 31.334978300705362 22.15896699070973 74-75 19.24100539199313 29.84086462757074 29.40715910356126 21.51097087687487 76-77 19.927452586375747 27.233536296673666 28.798245054123228 24.040766062827366 78-79 20.830848951366992 27.24354861460516 29.268814804071543 22.656787629956305 80-81 22.37378670143582 26.576711035544452 28.115174410922357 22.934327852097372 82-83 20.813141539978457 25.647334404746797 31.642541295110476 21.896982760164267 84-85 20.712077917403366 27.315596545922162 30.032611417038556 21.939714119635916 86-87 20.44156129750361 28.80692474879115 28.837266454857964 21.914247498847274 88-89 19.590439913040957 27.561977407667648 27.563707203945363 25.28387547534603 90-91 19.87296811018737 25.15005520186064 28.924661391468685 26.052315296483307 92-93 21.45425313857821 26.480929139641553 29.64453008265156 22.420287639128674 94-95 20.388222118529143 25.586380057580804 30.172744408774822 23.85265341511523 96-97 17.019806925424984 26.703460008366882 32.89767747415572 23.379055592052417 98-99 18.996281174772452 28.211099272166813 29.4011750588693 23.391444494191436 100-101 17.70535249238658 26.63532137049293 30.5659992367191 25.09332690040139 102-103 19.616417591139694 25.244754382713943 29.82641327264644 25.31241475349993 104-105 21.50201120501528 25.446648942589057 28.677797461625275 24.373542390770385 106-107 20.19578224627712 25.81302574803538 31.911007130053903 22.080184875633602 108-109 19.49466651186536 26.20512418828716 30.66745737265075 23.632751927196736 110-111 18.817142844646973 28.11787619127328 28.627276661669544 24.43770430241021 112-113 20.660849752017313 25.444127099949597 26.970177459969364 26.924845688063726 114-115 19.215659607770156 25.208205832984387 29.038704035751394 26.537430523494066 116-117 21.673880985124878 24.51147976560177 27.91148712410716 25.90315212516619 118-119 20.89601363147952 23.965860844119984 29.814983450869974 25.32314207353052 120-121 19.05352806954895 28.21787572204915 28.495103625933403 24.23349258246849 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6858.0 1 7671.5 2 5062.5 3 1588.5 4 1542.0 5 1554.0 6 1542.0 7 1622.5 8 1831.5 9 1900.5 10 1764.0 11 1723.5 12 1827.5 13 1953.0 14 2166.0 15 2436.0 16 2679.5 17 2951.0 18 3274.5 19 3744.5 20 4199.0 21 4819.0 22 5798.5 23 7026.0 24 8749.5 25 10791.5 26 13526.0 27 17196.5 28 21845.0 29 27235.5 30 32661.5 31 38310.0 32 44026.5 33 48791.0 34 53272.5 35 58779.0 36 64050.0 37 69533.5 38 75186.5 39 78110.0 40 79046.5 41 80025.0 42 81345.0 43 83553.5 44 84930.0 45 87580.5 46 98977.0 47 146471.0 48 248905.0 49 245302.0 50 140615.0 51 87857.5 52 65306.5 53 54174.5 54 46625.5 55 39149.0 56 31778.0 57 25100.0 58 19584.0 59 15499.0 60 12173.0 61 9246.0 62 6957.0 63 5024.0 64 3420.5 65 2217.5 66 1387.5 67 892.0 68 533.0 69 299.5 70 197.0 71 142.0 72 88.0 73 57.0 74 42.0 75 32.0 76 26.5 77 23.0 78 22.5 79 20.0 80 16.5 81 10.5 82 8.0 83 6.0 84 4.0 85 3.0 86 2.5 87 5.0 88 4.0 89 3.0 90 3.0 91 3.0 92 3.0 93 3.0 94 2.5 95 2.0 96 1.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48140858451629104 2 1.0224422512384503 3 1.0311400355074252 4 1.0108714432064205 5 1.0177588289397717 6 1.0278734468453214 7 0.9983167229267689 8 0.9854077942379738 9 0.9841090300711132 10-11 0.9774971397670963 12-13 0.9785991214844324 14-15 0.9777136004615729 16-17 0.999635165338582 18-19 1.0280111945599884 20-21 1.0190772713515845 22-23 0.9943613956913302 24-25 0.9869033408543585 26-27 0.9866672019149293 28-29 1.0079787411984131 30-31 1.0140002841538571 32-33 1.008982331690987 34-35 1.0190182366167273 36-37 1.027027282312367 38-39 1.0133312238254744 40-41 1.020061183599206 42-43 1.0119143901888994 44-45 0.9957388728380004 46-47 0.9895205474330321 48-49 0.9946959258555216 50-51 1.0169520208967218 52-53 0.9967818198204793 54-55 1.0095530007946074 56-57 1.0570169276198729 58-59 1.0196872969451098 60-61 1.0324584779192383 62-63 1.005794456008693 64-65 0.9935152311583756 66-67 0.995856942307715 68-69 1.0168536296719597 70-71 1.0153777613005273 72-73 1.020651530947779 74-75 1.0247249276529324 76-77 1.0383619514049676 78-79 1.0892498928519563 80-81 1.113985446757163 82-83 1.0896237795060524 84-85 1.0458396844868918 86-87 1.0306087228937095 88-89 1.028385081214085 90-91 1.0417269312918336 92-93 1.0297035236258978 94-95 1.0223438600136883 96-97 1.03117939199733 98-99 1.0644159477219872 100-101 1.0520186534019553 102-103 1.0276373079058922 104-105 1.0170700903664365 106-107 1.0189001671470126 108-109 1.0163813517931013 110-111 1.0139609276639523 112-113 1.0265943609234136 114-115 1.0302741927295183 116-117 1.0539471214072937 118-119 1.05068053274519 120-121 1.0295264194213258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 2540877.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.12030841297132 #Duplication Level Percentage of deduplicated Percentage of total 1 81.57106691681531 43.33080232196351 2 11.731256853082462 12.463359642150476 3 3.0153758784451337 4.805330899321195 4 1.2075162146917329 2.565745349523542 5 0.648711021900165 1.722986477711528 6 0.384914976910304 1.226808137174825 7 0.24683689443975226 0.917843637223778 8 0.19271195545010805 0.8189534806701223 9 0.13802184044656646 0.6598586459022777 >10 0.7611199397437485 7.5336499107876085 >50 0.05890405561381606 2.1560032853899362 >100 0.03620522992526003 3.672758722527087 >500 0.0026550287499848215 1.0354053238895409 >1k 0.0028826026428406134 3.195350689568708 >5k 0.0011378694642791894 4.105314613447803 >10k+ 6.827216785675137E-4 9.78982886274813 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 54889 2.1602383743880558 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 54570 2.147683654108404 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 34006 1.3383567957047902 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 29695 1.1686909677249233 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 18657 0.7342740321550394 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 15012 0.5908196264518117 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 13536 0.5327294473522332 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 12035 0.4736553560050329 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10548 0.41513225551650085 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9902 0.38970796303795896 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8332 0.32791827388732314 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8101 0.3188269247192997 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7811 0.30741354264688925 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 7729 0.30418631047469047 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7694 0.3028088333280202 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6791 0.26726992294392843 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 6082 0.23936617160138016 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5985 0.23554859208060838 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5918 0.23291170725698254 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5861 0.23066838733240533 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5732 0.2255914001346779 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5392 0.2122101935670243 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5297 0.20847132702606228 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5252 0.20670028498034337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4338 0.1707284532072981 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4254 0.16742250805528958 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4233 0.16659602176728744 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3923 0.15439550989677975 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3690 0.14522544774894652 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2836 0.11161500537019305 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2744 0.1079942082989456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2680 0.10547539294503433 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2582 0.10161845693435771 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.871297980972712E-5 0.0 5 0.0 0.0 0.0 7.871297980972712E-5 0.0 6 0.0 0.0 0.0 7.871297980972712E-5 0.0 7 0.0 0.0 0.0 7.871297980972712E-5 3.935648990486356E-5 8 0.0 0.0 0.0 7.871297980972712E-5 3.935648990486356E-5 9 0.0 0.0 0.0 1.1806946971459067E-4 3.935648990486356E-5 10-11 0.0 0.0 0.0 1.9678244952431778E-4 3.935648990486356E-5 12-13 0.0 0.0 0.0 1.9678244952431778E-4 3.935648990486356E-5 14-15 0.0 0.0 0.0 1.9678244952431778E-4 3.935648990486356E-5 16-17 0.0 0.0 0.0 4.1324314400106735E-4 3.935648990486356E-5 18-19 0.0 0.0 0.0 5.31312613715658E-4 3.935648990486356E-5 20-21 0.0 0.0 0.0 9.642340026691572E-4 3.935648990486356E-5 22-23 0.0 0.0 0.0 0.0018891115154334507 3.935648990486356E-5 24-25 0.0 0.0 0.0 0.003660153561152311 3.935648990486356E-5 26-27 0.0 0.0 0.0 0.006513499079254919 3.935648990486356E-5 28-29 0.0 0.0 0.0 0.013814127956607108 3.935648990486356E-5 30-31 0.0 0.0 0.0 0.03256749539627459 3.935648990486356E-5 32-33 0.0 0.0 0.0 0.07086136007370683 3.935648990486356E-5 34-35 0.0 0.0 0.0 0.1079942082989456 3.935648990486356E-5 36-37 0.0 0.0 0.0 0.13381206567653608 3.935648990486356E-5 38-39 0.0 0.0 0.0 0.1612632173851784 3.935648990486356E-5 40-41 0.0 3.935648990486356E-5 0.0 0.21179695042302324 3.935648990486356E-5 42-43 0.0 3.935648990486356E-5 0.0 0.27335050063422983 3.935648990486356E-5 44-45 0.0 3.935648990486356E-5 0.0 0.3249271806545535 3.935648990486356E-5 46-47 0.0 3.935648990486356E-5 0.0 0.38307639448898945 3.935648990486356E-5 48-49 0.0 3.935648990486356E-5 0.0 0.42591593375043346 3.935648990486356E-5 50-51 0.0 3.935648990486356E-5 0.0 0.4885714656789762 3.935648990486356E-5 52-53 0.0 3.935648990486356E-5 0.0 0.7244939444136809 3.935648990486356E-5 54-55 0.0 3.935648990486356E-5 0.0 0.9994777393789624 3.935648990486356E-5 56-57 0.0 3.935648990486356E-5 0.0 1.2232587409780167 3.935648990486356E-5 58-59 0.0 3.935648990486356E-5 0.0 1.5901792963610597 3.935648990486356E-5 60-61 0.0 3.935648990486356E-5 0.0 1.9776439394744414 3.935648990486356E-5 62-63 0.0 3.935648990486356E-5 0.0 2.130307763815407 3.935648990486356E-5 64-65 0.0 3.935648990486356E-5 0.0 2.254772663139538 3.935648990486356E-5 66-67 0.0 3.935648990486356E-5 0.0 2.93347533154891 3.935648990486356E-5 68-69 0.0 3.935648990486356E-5 0.0 3.4610884352135107 3.935648990486356E-5 70-71 0.0 3.935648990486356E-5 0.0 3.5782330274153376 3.935648990486356E-5 72-73 0.0 3.935648990486356E-5 0.0 3.8138012977408984 3.935648990486356E-5 74-75 0.0 3.935648990486356E-5 0.0 4.196129918921695 3.935648990486356E-5 76-77 0.0 3.935648990486356E-5 0.0 4.495376989913325 3.935648990486356E-5 78-79 0.0 3.935648990486356E-5 0.0 4.769553976835557 3.935648990486356E-5 80-81 0.0 3.935648990486356E-5 0.0 5.247597581464982 3.935648990486356E-5 82-83 0.0 3.935648990486356E-5 0.0 5.74589010015046 3.935648990486356E-5 84-85 0.0 3.935648990486356E-5 0.0 6.272460256832582 3.935648990486356E-5 86-87 3.935648990486356E-5 7.871297980972712E-5 0.0 7.3185951149937605 3.935648990486356E-5 88-89 3.935648990486356E-5 7.871297980972712E-5 0.0 8.857197731334496 3.935648990486356E-5 90-91 3.935648990486356E-5 7.871297980972712E-5 0.0 10.514125634574203 3.935648990486356E-5 92-93 3.935648990486356E-5 7.871297980972712E-5 0.0 12.109854196011849 3.935648990486356E-5 94-95 3.935648990486356E-5 7.871297980972712E-5 0.0 13.345333127105327 3.935648990486356E-5 96-97 3.935648990486356E-5 7.871297980972712E-5 0.0 14.485589030873985 3.935648990486356E-5 98-99 5.903473485729534E-5 7.871297980972712E-5 0.0 15.722268334909561 3.935648990486356E-5 100-101 7.871297980972712E-5 7.871297980972712E-5 0.0 16.788868567821268 3.935648990486356E-5 102-103 7.871297980972712E-5 7.871297980972712E-5 0.0 17.521174775481065 3.935648990486356E-5 104-105 7.871297980972712E-5 7.871297980972712E-5 0.0 18.41921116213024 3.935648990486356E-5 106-107 7.871297980972712E-5 7.871297980972712E-5 0.0 19.569758788009022 3.935648990486356E-5 108-109 7.871297980972712E-5 7.871297980972712E-5 0.0 20.68937221282258 3.935648990486356E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 325 0.0 47.821243 2 ATAACGC 440 0.0 40.50219 3 TACCTGG 2225 0.0 38.806255 2 AGTACGC 1130 0.0 38.205276 2 GTACGCA 1150 0.0 36.991623 3 GAGTACG 1210 0.0 36.226524 1 TACGCAG 1290 0.0 33.868298 4 ACCTGGG 2610 0.0 32.818237 3 ACGCAGT 1480 0.0 29.135977 5 CGCAGTG 1495 0.0 28.843065 6 GTACACG 360 0.0 28.838217 1 TAACGCA 620 0.0 27.816282 4 GAGTACT 7035 0.0 24.387033 12-13 TAGACCG 120 0.0015729911 23.956247 5 TCACGCA 505 0.0 22.767122 4 TACACGG 435 0.0 22.495735 2 GTATAAC 975 0.0 22.479021 1 GTACCTG 4040 0.0 22.413864 1 AGTACTT 7710 0.0 22.065617 12-13 TATCACG 525 0.0 21.928616 2 >>END_MODULE