##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003808_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2540877 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6022412733871 31.0 31.0 34.0 30.0 34.0 2 31.806658881952963 31.0 31.0 34.0 30.0 34.0 3 31.858378819596542 31.0 31.0 34.0 30.0 34.0 4 35.56446101090293 37.0 35.0 37.0 33.0 37.0 5 35.46517718094973 37.0 35.0 37.0 33.0 37.0 6 35.529733631340676 37.0 35.0 37.0 33.0 37.0 7 35.45801980969563 37.0 35.0 37.0 33.0 37.0 8 35.47067803754373 37.0 35.0 37.0 33.0 37.0 9 37.01062822009881 39.0 37.0 39.0 33.0 39.0 10-11 36.91280825478762 39.0 37.0 39.0 33.0 39.0 12-13 36.88236620662865 39.0 37.0 39.0 33.0 39.0 14-15 37.86318975692251 39.0 37.0 41.0 33.0 41.0 16-17 37.93685015055825 40.0 37.0 41.0 33.0 41.0 18-19 38.0055413937786 40.0 37.0 41.0 33.0 41.0 20-21 37.94131179903631 40.0 37.0 41.0 32.5 41.0 22-23 37.8466275620583 39.5 37.0 41.0 32.5 41.0 24-25 37.70308617851238 39.5 36.5 41.0 32.0 41.0 26-27 37.409770524114315 39.0 36.0 41.0 31.5 41.0 28-29 37.276984875694495 39.0 36.0 40.5 31.0 41.0 30-31 37.08265197410186 39.0 36.0 40.0 31.0 41.0 32-33 36.81821906373272 39.0 35.0 40.0 30.5 41.0 34-35 36.74219314827124 39.0 35.0 40.0 30.0 41.0 36-37 36.516611193694146 38.5 35.0 40.0 30.0 41.0 38-39 36.25770472163745 38.0 35.0 40.0 30.0 41.0 40-41 36.46790320822299 39.0 35.0 40.0 30.0 41.0 42-43 36.30748064546218 39.0 35.0 40.0 30.0 41.0 44-45 36.1229052016292 39.0 35.0 40.0 30.0 41.0 46-47 35.811820092039085 39.0 35.0 40.0 28.5 41.0 48-49 35.47994275204978 38.5 35.0 40.0 27.0 41.0 50-51 35.128612679795204 38.0 34.0 40.0 26.0 41.0 52-53 35.0202284486813 38.0 34.0 40.0 26.0 41.0 54-55 34.848790791525914 38.0 34.0 40.0 26.0 41.0 56-57 34.51184236781238 38.0 33.5 40.0 24.5 41.0 58-59 34.253296991550556 38.0 33.0 40.0 24.0 41.0 60-61 33.67752728683836 37.0 32.0 40.0 22.0 41.0 62-63 33.53098674197925 37.0 32.0 40.0 22.0 41.0 64-65 33.09920413306115 36.5 32.0 39.0 21.5 41.0 66-67 32.6969754931073 36.0 31.0 39.0 20.5 40.0 68-69 32.373159936510106 35.5 31.0 39.0 20.0 40.0 70-71 31.836294515633774 35.0 31.0 38.0 18.5 40.0 72-73 31.39793071447378 35.0 30.5 37.0 17.0 39.5 74-75 31.048945108322833 34.5 30.0 37.0 15.0 39.0 76-77 30.10693256698376 34.0 29.0 36.0 13.0 38.5 78-79 30.471270746281697 34.0 30.0 36.0 12.0 38.5 80-81 30.21417801806227 34.0 30.0 35.5 8.0 37.5 82-83 29.870757222801416 34.0 29.5 35.0 7.0 37.0 84-85 29.53345459067873 34.0 29.0 35.0 4.5 36.5 86-87 29.296751672749213 34.0 29.0 35.0 2.0 36.0 88-89 28.958675685599893 34.0 29.0 35.0 2.0 36.0 90-91 28.616705964121834 34.0 29.0 35.0 2.0 35.5 92-93 28.36619816701084 34.0 29.0 35.0 2.0 35.0 94-95 27.991244755255764 34.0 29.0 35.0 2.0 35.0 96-97 27.458707564356715 34.0 27.0 35.0 2.0 35.0 98-99 27.1599508752293 34.0 27.0 35.0 2.0 35.0 100-101 26.125145963381936 33.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08873497161287602 1101 2 0.12771943690917098 1101 3 0.11007200129974137 1101 4 0.20201550546448033 1101 5 0.21494486468105833 1101 6 0.22272930640776423 1101 7 0.16161919259614876 1101 8 0.17018369089899466 1101 9 0.2527856196395035 1101 10-11 0.21277053709425786 1101 12-13 0.2593350834946264 1101 14-15 0.2957069837928614 1101 16-17 0.250234369899502 1101 18-19 0.12976946384296895 1101 20-21 0.09852479913967471 1101 22-23 0.1982784616209372 1101 24-25 0.17826351239553873 1101 26-27 0.2837117850003352 1101 28-29 0.23412857493932648 1101 30-31 0.20968825735296548 1101 32-33 0.31479603115506194 1101 34-35 0.3368888642710459 1101 36-37 0.2858309303087694 1101 38-39 0.1800205417001166 1101 40-41 0.32437602233744656 1101 42-43 0.21602633151263007 1101 44-45 0.2929371968089356 1101 46-47 0.2786933279256587 1101 48-49 0.2802172511644869 1101 50-51 0.29993542344743673 1101 52-53 0.4487585660134954 1101 54-55 0.48195408697205266 1101 56-57 0.4042313688025061 1101 58-59 0.2292199017871397 1101 60-61 0.27115011207894923 1101 62-63 0.13176251191453758 1101 64-65 0.011751552150592204 1101 66-67 -0.04634751046653207 1101 68-69 -0.059838013111082944 1101 70-71 -0.057136805836986326 1101 72-73 0.031142444542773262 1101 74-75 -0.0439920603314512 1101 76-77 -0.03307562149564447 1101 78-79 0.09396349956039884 1101 80-81 0.14557820718574987 1101 82-83 0.0805734654659851 1101 84-85 -0.08374539351394716 1101 86-87 -0.1308401661053118 1101 88-89 -0.027503192698400625 1101 90-91 0.08025456726810276 1101 92-93 0.1344963836616735 1101 94-95 -0.005706805875835386 1101 96-97 0.17644456211635173 1101 98-99 -0.010123654087621503 1101 100-101 0.22232621676949904 1102 1 -0.004909333324221166 1102 2 0.033169661470278555 1102 3 -0.04004770809436664 1102 4 0.0033913093952193663 1102 5 -0.05494531629751975 1102 6 0.004872218852156607 1102 7 -0.07269698423407789 1102 8 -0.09359222923870902 1102 9 0.07149427713201106 1102 10-11 0.044957776792990956 1102 12-13 0.08453810772677883 1102 14-15 0.12735257565817193 1102 16-17 0.07478084199324542 1102 18-19 -0.041107738970872276 1102 20-21 -0.005562095059637784 1102 22-23 -0.039007161970260995 1102 24-25 -0.11690063854275934 1102 26-27 -0.17018111140660608 1102 28-29 0.14634580281828136 1102 30-31 0.0018762024535377009 1102 32-33 -0.003456019978948177 1102 34-35 -0.18440075282849477 1102 36-37 -0.2412000309327169 1102 38-39 -0.1868250930300306 1102 40-41 0.14514988501217374 1102 42-43 0.15190542809688878 1102 44-45 0.13680528863307728 1102 46-47 0.10317417552863617 1102 48-49 0.020415053702606656 1102 50-51 0.09490024306734313 1102 52-53 0.21277437193017334 1102 54-55 0.09912065495619515 1102 56-57 0.23080611694086883 1102 58-59 0.026673720702376613 1102 60-61 0.07222145032453398 1102 62-63 -0.02739924459009302 1102 64-65 -0.12790959198168395 1102 66-67 -0.10318692193036583 1102 68-69 -0.14556528854198092 1102 70-71 -0.017386122371430446 1102 72-73 0.0756326540815273 1102 74-75 -0.011137539996092016 1102 76-77 -0.06669729704373495 1102 78-79 0.026616427358959527 1102 80-81 0.03612694098946179 1102 82-83 -0.07412717748197295 1102 84-85 -0.11513491468373971 1102 86-87 -0.30542011406287983 1102 88-89 -0.21150756717288388 1102 90-91 -0.12258803005584085 1102 92-93 -0.0578256492326652 1102 94-95 -0.2801425888706355 1102 96-97 -0.1252975223288466 1102 98-99 -0.221395680382642 1102 100-101 -0.21529469389689027 1103 1 -0.03336904596118018 1103 2 0.015430825868975262 1103 3 0.039313831916810926 1103 4 0.016499432285918658 1103 5 0.0344363473581808 1103 6 0.02901734303821968 1103 7 0.10314134751795478 1103 8 -0.03862505742307576 1103 9 -0.03760758338448511 1103 10-11 0.031676157868538724 1103 12-13 0.06668135168426659 1103 14-15 0.05964478386533045 1103 16-17 -0.017131101898826273 1103 18-19 0.027219439754823327 1103 20-21 0.04635735458359136 1103 22-23 0.017021564586457316 1103 24-25 0.05494505057833976 1103 26-27 0.022969525120075218 1103 28-29 -0.07130466691393877 1103 30-31 0.011179123414613912 1103 32-33 0.04213837215268512 1103 34-35 -0.026356967655431163 1103 36-37 -0.028062759625264277 1103 38-39 -8.669264911702612E-4 1103 40-41 -0.04889212715732327 1103 42-43 0.04314135345961745 1103 44-45 0.12491874569759176 1103 46-47 0.17970582802860235 1103 48-49 0.23747686892465225 1103 50-51 0.17408564703487883 1103 52-53 0.08822561054157063 1103 54-55 0.10131782010004997 1103 56-57 0.13316697623564977 1103 58-59 0.010383302828074648 1103 60-61 0.023572566764407554 1103 62-63 -0.009894083709241386 1103 64-65 -0.1213105149832927 1103 66-67 -0.21652565983777095 1103 68-69 -0.1377488962197262 1103 70-71 0.13010378331387784 1103 72-73 0.05813108090640995 1103 74-75 -0.08435746933588817 1103 76-77 -0.06824779515267565 1103 78-79 -0.01229921363002262 1103 80-81 -0.018036178587010454 1103 82-83 -0.04234944627667758 1103 84-85 -0.18595236734227072 1103 86-87 -0.21326732003888793 1103 88-89 -0.026934187353713668 1103 90-91 0.0019908545223863428 1103 92-93 -0.17231407759552653 1103 94-95 -0.37048426842596527 1103 96-97 -0.03520136369214555 1103 98-99 -0.23804696729946073 1103 100-101 -0.22729056031834816 1104 1 -0.04998274024062965 1104 2 -0.10678958766697377 1104 3 -0.09804177246026313 1104 4 -0.038965233531314425 1104 5 -0.07804292431638515 1104 6 -0.046878776431881874 1104 7 -0.14736397860770012 1104 8 -0.20392349846562752 1104 9 -0.16227206557451979 1104 10-11 -0.14652800793933096 1104 12-13 -0.15622660986644377 1104 14-15 -0.09798915390753393 1104 16-17 -0.11075784448347292 1104 18-19 -0.1531102577287342 1104 20-21 -0.1858569809857471 1104 22-23 -0.14979636810855368 1104 24-25 -0.1027412935540184 1104 26-27 -0.10233759371295292 1104 28-29 -0.1332129727656408 1104 30-31 -0.10314373957700695 1104 32-33 -0.10220367332315305 1104 34-35 0.0022718360508378055 1104 36-37 -0.11724403761436264 1104 38-39 -0.11533793259958713 1104 40-41 -0.05595586031702737 1104 42-43 0.031303957218348444 1104 44-45 0.04162249693894182 1104 46-47 -0.022077686277455655 1104 48-49 -0.10439557809514355 1104 50-51 -0.05071831219238021 1104 52-53 -0.08014636101494688 1104 54-55 -0.22696439897843845 1104 56-57 -0.2783707961603099 1104 58-59 -0.19512838232622443 1104 60-61 -0.22964383695310886 1104 62-63 -0.25972837421496564 1104 64-65 -0.29548758875271375 1104 66-67 -0.4192920129641209 1104 68-69 -0.3156479926052711 1104 70-71 -0.2984678125563853 1104 72-73 -0.11009889576544296 1104 74-75 -0.22989080797816897 1104 76-77 -0.27070410171641157 1104 78-79 -0.1484573766085333 1104 80-81 -0.20595133064157167 1104 82-83 -0.1664026185302312 1104 84-85 -0.2835098796621587 1104 86-87 -0.35704255706787436 1104 88-89 -0.3158981692533196 1104 90-91 -0.3309449907248556 1104 92-93 -0.47155966023713347 1104 94-95 -0.2725470295794459 1104 96-97 -0.2867726761581828 1104 98-99 -0.40838695868022157 1104 100-101 -0.3213816408145185 1105 1 0.06841492309561659 1105 2 0.061596814295640456 1105 3 0.08376811559597996 1105 4 0.14810364473608217 1105 5 0.05903899718756378 1105 6 0.13461572188817428 1105 7 0.09346227835403909 1105 8 0.1294167247376521 1105 9 0.18534230507862048 1105 10-11 0.16628568902708452 1105 12-13 0.10914819430682599 1105 14-15 0.20931662659831574 1105 16-17 0.16334452425381585 1105 18-19 0.15923035463340085 1105 20-21 0.13874748108409563 1105 22-23 0.19651278795840454 1105 24-25 0.2516854954007073 1105 26-27 0.29055159722150137 1105 28-29 0.21092248872972164 1105 30-31 0.2614726324645673 1105 32-33 0.2220659767047195 1105 34-35 0.2607167971563342 1105 36-37 0.08451202392691215 1105 38-39 0.18742249379308618 1105 40-41 0.1651884734301774 1105 42-43 0.2742132119292364 1105 44-45 0.345627198206067 1105 46-47 0.29177572770061744 1105 48-49 0.400633908172388 1105 50-51 0.3377308327272388 1105 52-53 0.3205595061607127 1105 54-55 0.284930248733005 1105 56-57 0.3602510112759063 1105 58-59 0.2839197607587849 1105 60-61 0.23523831776105908 1105 62-63 0.24667492836103833 1105 64-65 0.174250855153538 1105 66-67 0.2278998423543115 1105 68-69 0.1909972869547687 1105 70-71 0.15450454861154483 1105 72-73 0.15421552178633746 1105 74-75 0.33402906948667876 1105 76-77 0.31402953828741076 1105 78-79 0.29476819440725777 1105 80-81 0.3967502586376028 1105 82-83 0.4369089024251913 1105 84-85 0.3163385907154428 1105 86-87 0.24966305637050823 1105 88-89 0.34398981571630927 1105 90-91 0.4972910861546893 1105 92-93 0.5192332758880731 1105 94-95 0.3941376932981093 1105 96-97 0.4342476399300459 1105 98-99 0.38855568529073636 1105 100-101 0.5638519542863598 1106 1 -0.04991724168235834 1106 2 0.017136350219068675 1106 3 0.03239327505665557 1106 4 -0.019705991760908148 1106 5 -0.02407401632967776 1106 6 -0.05436122510310071 1106 7 0.07133304181719069 1106 8 0.03471212086893871 1106 9 -0.035445531651596696 1106 10-11 -0.0693769895890668 1106 12-13 -0.13167131461265313 1106 14-15 -0.06783163749100396 1106 16-17 -0.005565065471046182 1106 18-19 0.04600878666253294 1106 20-21 -0.05762413208786654 1106 22-23 -8.971859031703389E-6 1106 24-25 0.0019768113137388355 1106 26-27 -0.09470225160484347 1106 28-29 -0.10977705548879868 1106 30-31 -0.07252487721080314 1106 32-33 0.02345854756540433 1106 34-35 -0.17664242633638594 1106 36-37 -0.14770962573809499 1106 38-39 0.04507584311811996 1106 40-41 -0.1695202690097517 1106 42-43 9.225271258728185E-4 1106 44-45 0.10632060189809778 1106 46-47 0.031513462582488216 1106 48-49 -0.018881878995522072 1106 50-51 0.04059600777264194 1106 52-53 -0.00790659211823197 1106 54-55 0.025610608294940107 1106 56-57 0.0888666726707541 1106 58-59 0.03188010049867529 1106 60-61 0.18472193898393385 1106 62-63 -0.09369562188938829 1106 64-65 -0.004704644881933007 1106 66-67 -0.10786845511290011 1106 68-69 0.020786875171417307 1106 70-71 0.215526030579138 1106 72-73 0.16554721704745035 1106 74-75 0.1318030837481423 1106 76-77 0.06328980234800241 1106 78-79 0.25836178491852735 1106 80-81 0.1059604199521047 1106 82-83 0.08414440867299433 1106 84-85 0.052197649473274765 1106 86-87 0.06438411577292058 1106 88-89 -0.06545986204070786 1106 90-91 -0.19223370532326456 1106 92-93 -0.22306974473027452 1106 94-95 0.0929810030418814 1106 96-97 0.12988288367931844 1106 98-99 -0.38534231912792904 1106 100-101 -0.4232899717741603 1107 1 -0.20382926105468258 1107 2 -0.1055876176765409 1107 3 -0.21311256357032704 1107 4 -0.1160090595733223 1107 5 -0.11883856209558274 1107 6 -0.06942606808535601 1107 7 -0.13248821759133733 1107 8 -0.060896659167475775 1107 9 -0.2146105618348173 1107 10-11 -0.14994537147247655 1107 12-13 -0.13676881260926876 1107 14-15 -0.15691474062415267 1107 16-17 -0.19810797229172294 1107 18-19 -0.1956564825064504 1107 20-21 -0.23057143559104532 1107 22-23 -0.2791274066470564 1107 24-25 -0.25855112598513585 1107 26-27 -0.37072547938203115 1107 28-29 -0.4992612996355632 1107 30-31 -0.2580633162268313 1107 32-33 -0.2653789601435932 1107 34-35 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64-65 -0.5536668141150827 2316 66-67 -0.5135821139335377 2316 68-69 -0.6222919449051005 2316 70-71 -0.6853913317289191 2316 72-73 -0.5065995408993835 2316 74-75 -0.4678511117077626 2316 76-77 -0.5283690082845887 2316 78-79 -0.5386743906584108 2316 80-81 -0.5892876666517033 2316 82-83 -0.5453608530622525 2316 84-85 -0.5061535511233934 2316 86-87 -0.4899835332734277 2316 88-89 -0.5501268301566107 2316 90-91 -0.660434036694955 2316 92-93 -0.6648688794263578 2316 94-95 -0.8287124965001027 2316 96-97 -0.5991359991676077 2316 98-99 -0.8183080456845495 2316 100-101 -1.1021237303066478 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 16.0 5 50.0 6 127.0 7 365.0 8 870.0 9 1732.0 10 2960.0 11 5105.0 12 7917.0 13 10972.0 14 13142.0 15 16982.0 16 21163.0 17 27533.0 18 24522.0 19 17491.0 20 15628.0 21 15756.0 22 16731.0 23 18698.0 24 21198.0 25 25719.0 26 31824.0 27 39613.0 28 50030.0 29 61918.0 30 76243.0 31 93719.0 32 115461.0 33 144502.0 34 185215.0 35 244862.0 36 342474.0 37 438637.0 38 378712.0 39 72922.0 40 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.44995497150426 19.966820279401126 14.092363742039288 19.49086100705532 2 14.331685253159126 24.880605368369928 37.498140301188556 23.28956907728239 3 25.51874008480653 25.199130750812216 29.14205546168921 20.14007370269205 4 12.336134334719864 16.580810483939207 41.25433856105589 29.828716620285046 5 11.599616982640246 41.03878306584695 34.188667928435734 13.172932023077072 6 27.679964736140928 39.973197892826995 16.783156784455567 15.563680586576512 7 26.94093417351568 33.95197799814788 21.98894318772613 17.118144640610307 8 24.065037386697586 31.87493924341871 19.280272126513797 24.77975124336991 9 22.913623918040894 22.189779355710645 23.396449336193765 31.500147390054696 10-11 24.963983800040182 25.878099651280927 27.797127008243827 21.36078954043506 12-13 29.864668773813136 23.63408382223933 26.195699358922138 20.305548045025397 14-15 22.848989068204713 26.973478229982334 29.36247035627959 20.815062345533363 16-17 19.835631555561328 29.21237431012993 29.58464734814003 21.36734678616871 18-19 19.039410408296035 32.61019718782137 29.34463179445522 19.005760609427373 20-21 22.093131269714938 27.238164738371385 28.241417531230983 22.427286460682694 22-23 20.00557287897053 28.285784790054773 28.560473411345765 23.14816891962893 24-25 23.30457161051086 28.20677663657076 28.466726252392384 20.021925500526 26-27 23.183886604188974 24.917527438866593 32.34658175021898 19.55200420672545 28-29 19.890586796103666 31.273429099078815 29.909778780498176 18.926205324319344 30-31 21.52023142204945 27.508822089871465 28.827446790463583 22.1434996976155 32-33 21.476791521810064 28.56010730770003 29.59872572106217 20.364375449427744 34-35 23.72454175603656 26.46628130111156 30.130698001282713 19.678478941569157 36-37 22.8728176095727 25.811971464235228 31.68612626953882 19.629084656653248 38-39 21.118818334751623 28.26013198363548 31.791221663390562 18.82982801822234 40-41 20.910919094579775 29.228323942874734 29.434482362286175 20.426274600259315 42-43 19.64620876964922 30.22787014090017 27.578824161893706 22.547096927556904 44-45 22.014741366858765 26.58698158155629 27.823090216488243 23.5751868350967 46-47 23.784616759065923 29.410551599242073 27.555265891618852 19.249565750073153 48-49 23.623752743639304 28.329647598053743 28.192037631101385 19.854562027205567 50-51 19.562162985457384 29.249172628190973 31.30177100268923 19.886893383662414 52-53 21.82533825919161 31.02293027171327 27.003117427565364 20.148614041529754 54-55 20.12114321157616 28.92877144387548 27.845031459610205 23.105053884938155 56-57 22.56825341663113 27.14071134251283 28.368118697589995 21.922916543266048 58-59 25.970267675926877 26.458190911699177 26.654324442287702 20.91721697008624 60-61 22.07866072950283 28.394474233913392 30.71523852370713 18.811626512876646 62-63 20.868396844884234 28.052311564336126 30.141322073647448 20.937969517132196 64-65 19.851894024851532 32.33319670856362 27.75036095319204 20.06454831339281 66-67 21.601566582615128 27.72065103167669 26.98751115268965 23.690271233018535 68-69 20.446403024508008 29.426001527839386 27.162441756535134 22.965153691117475 70-71 23.13432321834964 28.940489813026453 26.667427561649713 21.25775940697419 72-73 22.913289387876706 25.842770035700273 29.90670150503153 21.337239071391494 74-75 20.290364425497348 31.899805421437332 28.04192333667601 19.767906816389306 76-77 21.745916862563593 27.964006128592604 27.07411653535374 23.215960473490057 78-79 21.57778593769002 29.034620723474614 27.373521032304986 22.014072306530384 80-81 23.310223853855156 27.840530620043907 27.40365381788529 21.44559170821565 82-83 22.33366668280283 26.84405817361486 29.69588846685613 21.126386676726185 84-85 20.5189354061101 28.71687902789393 29.333753136222757 21.43043242977321 86-87 21.033936107535116 29.226900466495042 26.825708430560795 22.91345499540904 88-89 20.29067129184136 30.048168407994563 26.324611541605513 23.33654875855856 90-91 22.072929937183105 25.718718379520144 27.158083606565764 25.050268076730987 92-93 22.268984291644184 28.525505170065298 27.34254747474986 21.86296306354066 94-95 21.812372078983046 28.039421073335184 28.084287515971372 22.0639193317104 96-97 18.648995039694057 28.558036091160062 31.090395559161493 21.702573309984388 98-99 20.679382407536956 30.123109576988792 27.111991125861984 22.085516889612265 100-101 18.347970132900095 29.15779170484278 27.448033261231647 25.046204901025483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 433.0 1 307.5 2 353.5 3 521.0 4 424.0 5 362.0 6 525.0 7 950.0 8 1476.5 9 1753.5 10 1762.0 11 1768.0 12 1951.5 13 2208.5 14 2465.5 15 2806.0 16 3176.0 17 3494.0 18 3858.5 19 4343.0 20 4938.5 21 5600.5 22 6398.5 23 7631.0 24 9254.5 25 11200.5 26 13842.5 27 17553.0 28 22114.5 29 27376.5 30 33005.0 31 38259.0 32 43643.5 33 48414.5 34 52685.0 35 57812.5 36 62580.0 37 67721.0 38 73126.5 39 75907.0 40 76915.0 41 77904.0 42 79395.5 43 82047.5 44 83167.0 45 84875.5 46 94021.0 47 144007.5 48 263442.5 49 261726.0 50 145795.0 51 90757.5 52 65588.0 53 53820.0 54 46598.0 55 39537.0 56 32409.0 57 25678.5 58 20086.0 59 16023.0 60 12652.5 61 9633.0 62 7129.5 63 5079.0 64 3437.5 65 2203.0 66 1345.5 67 785.5 68 450.0 69 242.5 70 129.5 71 80.0 72 46.5 73 25.0 74 15.5 75 12.0 76 8.0 77 7.5 78 5.5 79 3.5 80 2.0 81 1.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.054075817129282534 2 0.005627978056395489 3 0.04777877874450436 4 0.0 5 0.0 6 0.0012594076769556338 7 0.0 8 0.0 9 0.0 10-11 2.558171843816131E-4 12-13 0.0 14-15 0.0030501279676269258 16-17 0.0 18-19 0.0 20-21 0.011452738562315296 22-23 0.0 24-25 0.0 26-27 0.007221915897542463 28-29 0.009170062147833209 30-31 0.009347166352405095 32-33 0.00574604752611008 34-35 0.006788994508588963 36-37 0.01912725409376369 38-39 0.010193330885359661 40-41 0.013971553916226561 42-43 0.0 44-45 0.0 46-47 5.903473485729533E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0013184424118129292 58-59 2.3613893942918133E-4 60-61 0.01062625227431316 62-63 0.01448318828498979 64-65 0.042268870157823456 66-67 0.02245287749072466 68-69 0.001338120656765361 70-71 3.7388665409620383E-4 72-73 0.0 74-75 3.935648990486356E-5 76-77 0.0 78-79 0.0 80-81 1.967824495243178E-5 82-83 0.0 84-85 7.871297980972712E-5 86-87 5.509908586680898E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 9.839122476215889E-5 96-97 2.1646069447674954E-4 98-99 4.1324314400106735E-4 100-101 8.264862880021347E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2540877.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.31048867895546 #Duplication Level Percentage of deduplicated Percentage of total 1 79.73839917571405 39.319394298320795 2 12.636682832386564 12.46242011491897 3 3.348385983618968 4.953316474140489 4 1.3774963421422253 2.7170007113802703 5 0.7308370042823988 1.801896491291448 6 0.4760585692575569 1.4084808413936696 7 0.2885895691453255 0.9961344877543645 8 0.2196242861627405 0.8663824701161192 9 0.16669871245600038 0.7397995476022254 >10 0.891721192675181 8.237318667707026 >50 0.0753090935121307 2.5708911859943098 >100 0.04144636516208007 4.011082942352503 >500 0.0033718962051081523 1.1438969775229135 >1k 0.0037733124200019326 4.29360216269948 >5k 5.619827008513517E-4 2.1512547380367555 >10k+ 0.0010436821587239387 12.327127888768613 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 96471 3.796759937612092 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 28098 1.1058386533468563 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 27397 1.078249753923547 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 24786 0.9754899587819481 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 20185 0.7944107487296709 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 18971 0.7466319699851666 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 18312 0.7206960431378614 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 17673 0.6955472460886536 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 13962 0.549495312051705 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 12599 0.49585241631137594 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 11679 0.45964444559890144 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 10699 0.4210750854921352 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10553 0.41532903796602505 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8966 0.35287028848700663 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8721 0.34322794846031507 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8453 0.33268040916581165 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7875 0.3099323580008005 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7481 0.2944259009782843 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 7018 0.27620384615233246 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5827 0.22933026667563994 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4966 0.19544432886755242 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4749 0.18690397055819702 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4736 0.1863923361894338 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4664 0.18355866891628364 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4597 0.18092178409265777 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4337 0.17068909671739324 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 4131 0.16258165979699135 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4097 0.161243539140226 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3966 0.15608783896268885 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3923 0.15439550989677975 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3689 0.14518609125904167 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3460 0.1361734550708279 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3133 0.12330388287193754 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.935648990486356E-5 0.0 5 0.0 0.0 0.0 3.935648990486356E-5 0.0 6 0.0 0.0 0.0 3.935648990486356E-5 0.0 7 0.0 0.0 0.0 3.935648990486356E-5 0.0 8 0.0 0.0 0.0 3.935648990486356E-5 0.0 9 0.0 0.0 0.0 7.871297980972712E-5 0.0 10-11 0.0 0.0 0.0 1.1806946971459067E-4 0.0 12-13 0.0 0.0 0.0 1.77104204571886E-4 0.0 14-15 0.0 0.0 0.0 1.9678244952431778E-4 0.0 16-17 0.0 0.0 0.0 3.738866540962038E-4 0.0 18-19 0.0 0.0 0.0 5.509908586680898E-4 0.0 20-21 0.0 0.0 0.0 0.0010035904925740206 0.0 22-23 0.0 0.0 0.0 0.0020662157200053368 0.0 24-25 0.0 0.0 0.0 0.003915970745533924 0.0 26-27 0.0 0.0 0.0 0.00696609871316085 0.0 28-29 0.0 0.0 0.0 0.014620935999656812 0.0 30-31 0.0 0.0 0.0 0.03445660691170804 0.0 32-33 0.0 0.0 0.0 0.07438376592019212 0.0 34-35 0.0 0.0 0.0 0.1131302302315303 0.0 36-37 0.0 0.0 0.0 0.13985328687693266 0.0 38-39 0.0 0.0 0.0 0.16856384626253063 0.0 40-41 0.0 0.0 0.0 0.22092765608095158 0.0 42-43 0.0 0.0 0.0 0.28474420446168786 0.0 44-45 0.0 0.0 0.0 0.33893809106068495 0.0 46-47 0.0 0.0 0.0 0.3984647820417911 0.0 48-49 0.0 0.0 0.0 0.44191434689676046 0.0 50-51 0.0 0.0 0.0 0.5052979738885433 0.0 52-53 0.0 0.0 0.0 0.7443492935706845 0.0 54-55 0.0 0.0 0.0 1.0231900245466428 0.0 56-57 0.0 0.0 0.0 1.2492340243152267 0.0 58-59 0.0 0.0 0.0 1.6216054535500932 0.0 60-61 0.0 0.0 0.0 2.0151113178638713 0.0 62-63 0.0 0.0 0.0 2.171730469440276 0.0 64-65 0.0 0.0 0.0 2.297612202400982 0.0 66-67 0.0 0.0 0.0 2.9898141468477224 0.0 68-69 0.0 0.0 0.0 3.5299622925470224 0.0 70-71 0.0 0.0 0.0 3.651199959698954 0.0 72-73 0.0 0.0 0.0 3.893655615757866 0.0 74-75 0.0 0.0 0.0 4.288952200362315 0.0 76-77 0.0 0.0 0.0 4.601147556532647 0.0 78-79 0.0 0.0 0.0 4.885675300299857 0.0 80-81 0.0 0.0 0.0 5.381881925020377 0.0 82-83 0.0 0.0 0.0 5.897412586284185 0.0 84-85 0.0 0.0 0.0 6.441122494319875 0.0 86-87 0.0 0.0 0.0 7.5235440361733374 0.0 88-89 0.0 0.0 0.0 9.108311815172478 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 410 0.0 46.353603 2 TACCTGG 2105 0.0 43.562477 2 ATAACGC 455 0.0 41.765068 3 TAACGCA 525 0.0 37.088158 4 ACCTGGG 2380 0.0 36.52908 3 AGTACGC 1195 0.0 33.39787 2 TACGCAG 1255 0.0 31.40835 4 GAGTACG 1375 0.0 31.122952 1 GTATAAC 780 0.0 31.089699 1 GTACGCA 1300 0.0 30.331879 3 GTACCTG 3130 0.0 29.926977 1 GTACACG 280 0.0 27.17083 1 TCGATCG 105 6.914348E-5 27.139814 9 ACGCAGT 1510 0.0 26.733309 5 TACACGG 240 9.094947E-12 25.735907 2 CGCAGTG 1580 0.0 25.548923 6 ACCGATC 100 0.0016562231 23.744999 8 CCTGGGG 2085 0.0 21.183008 4 AGTACTT 10940 0.0 20.773495 12-13 GAGTACT 10585 0.0 20.505497 12-13 >>END_MODULE