FastQCFastQC Report
Wed 25 May 2016
SRR1003807_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003807_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303790
Sequences flagged as poor quality0
Sequence length121
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT200470.6067879617045878No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT187190.5665917022571046No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT125650.38032078310062084No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC124640.37726368806734084No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT80590.24393196904161585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66450.2011326385756964No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT62580.18941881899273258No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC61210.1852720663238281No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55160.16695976439180457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51930.15718311393883994No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC47990.14525741648228246No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC43220.1308194528102573No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41060.12428150699651007No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG39340.11907536495963728No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT35360.10702859443245485No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA34780.10527303490839308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG26700.034.4703142
ACCTGGG28500.030.6420173
CATGGGT36450.025.6922474
GTACCCG2959.094947E-1225.3931161
GTACATG130350.025.3301771
TAACGCA5550.024.84454
TACATGG127050.024.2223192
ACATGGG128050.023.017413
TATAACG5500.023.0089382
CTGCGCG1250.002009563422.968889
GAGTACT88650.022.74976312-13
GTACCGG2854.642061E-922.2403951
GTACACG3259.113137E-1021.2761231
AGTACTT97150.021.17330712-13
GTACCTG45750.020.9079591
ATAACGC6950.020.6668283
TCACGCA4750.020.5620924
GTACTAG10800.020.2747041
TCTCGGG14050.020.051662114-115
GTACTTT100300.020.05143414-15
Produced by FastQC (version 0.11.5)