##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003807_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3303790 Sequences flagged as poor quality 0 Sequence length 121 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.921565232657038 31.0 31.0 34.0 28.0 34.0 2 30.869186600843275 31.0 31.0 34.0 27.0 34.0 3 30.929256096785814 31.0 31.0 34.0 27.0 34.0 4 34.419547549935075 37.0 35.0 37.0 30.0 37.0 5 34.386279394271426 37.0 35.0 37.0 30.0 37.0 6 34.49293538632904 37.0 35.0 37.0 31.0 37.0 7 34.53201171987324 37.0 35.0 37.0 31.0 37.0 8 34.500397725037004 37.0 35.0 37.0 30.0 37.0 9 35.980626492603946 39.0 35.0 39.0 31.0 39.0 10-11 35.82354901491923 39.0 35.0 39.0 30.0 39.0 12-13 35.6825978648764 39.0 35.0 39.0 30.0 39.0 14-15 36.71481086872955 39.0 36.0 41.0 30.0 41.0 16-17 36.79529737059559 39.0 36.0 41.0 31.0 41.0 18-19 36.784048017579806 39.5 36.0 41.0 30.5 41.0 20-21 36.674113064087 39.0 36.0 41.0 30.0 41.0 22-23 36.60354895438269 39.0 36.0 41.0 30.0 41.0 24-25 36.43438853559094 39.0 36.0 41.0 29.5 41.0 26-27 36.26077353584822 39.0 36.0 41.0 29.0 41.0 28-29 36.091317547422804 39.0 36.0 40.5 28.0 41.0 30-31 35.90747202455362 39.0 35.0 40.0 28.0 41.0 32-33 35.68892650561931 38.0 35.0 40.0 27.0 41.0 34-35 35.69771595652266 39.0 35.0 40.0 27.0 41.0 36-37 35.59637310482809 38.5 35.0 40.0 27.0 41.0 38-39 35.6343587213473 38.5 35.0 40.0 27.0 41.0 40-41 35.721590203977854 39.0 35.0 41.0 27.0 41.0 42-43 35.59130453206772 39.0 35.0 41.0 26.5 41.0 44-45 35.39459121191117 39.0 35.0 41.0 25.5 41.0 46-47 35.14733291159547 39.0 35.0 41.0 24.5 41.0 48-49 34.923672206768586 39.0 34.5 40.0 24.0 41.0 50-51 34.21278501357531 38.0 33.5 39.5 23.5 40.5 52-53 34.37922613120083 38.0 33.5 40.0 24.0 40.5 54-55 34.73486904433999 38.5 34.0 40.0 24.0 41.0 56-57 34.59277617524116 38.0 34.0 40.0 23.5 41.0 58-59 34.3572469799836 38.0 34.0 40.0 23.0 41.0 60-61 34.132753897796164 38.0 34.0 40.0 22.0 41.0 62-63 33.91144201053941 38.0 33.0 40.0 22.0 41.0 64-65 33.64594556554745 37.5 33.0 40.0 21.5 41.0 66-67 33.325486032707886 37.0 33.0 40.0 20.0 41.0 68-69 32.995014513634345 36.5 32.0 39.0 19.5 41.0 70-71 32.62973145992936 36.0 32.0 39.0 18.0 41.0 72-73 32.301129611748934 35.5 31.5 39.0 18.0 40.5 74-75 31.93398051934294 35.0 31.0 38.5 15.5 40.0 76-77 31.51535221669658 35.0 31.0 37.5 12.5 39.5 78-79 30.98059819177369 35.0 30.5 37.0 8.0 39.0 80-81 30.578986406520997 35.0 30.0 36.5 7.0 39.0 82-83 30.238165561370426 34.0 30.0 36.0 7.0 38.0 84-85 29.975447743349307 34.0 30.0 36.0 7.0 37.0 86-87 29.734004582615725 34.0 30.0 35.0 2.0 37.0 88-89 29.41034493717821 34.0 29.0 35.0 2.0 36.5 90-91 29.064570538684357 34.0 29.0 35.0 2.0 36.0 92-93 28.80631562538781 34.0 29.0 35.0 2.0 36.0 94-95 28.58665002920888 34.0 29.0 35.0 2.0 35.5 96-97 28.3282605734626 34.0 29.0 35.0 2.0 35.0 98-99 28.08943380178522 34.0 28.5 35.0 2.0 35.0 100-101 27.834344343920165 34.0 27.0 35.0 2.0 35.0 102-103 27.57229696802763 34.0 26.5 35.0 2.0 35.0 104-105 27.313235405398043 34.0 25.5 35.0 2.0 35.0 106-107 27.013389168197737 33.5 25.0 35.0 2.0 35.0 108-109 26.73129194046837 33.0 25.0 35.0 2.0 35.0 110-111 26.399803104918895 33.0 25.0 35.0 2.0 35.0 112-113 25.973814618967914 33.0 23.5 35.0 2.0 35.0 114-115 25.51454375126748 32.5 20.5 35.0 2.0 35.0 116-117 24.9356973354844 32.0 18.0 35.0 2.0 35.0 118-119 24.25350612478396 32.0 3.5 34.0 2.0 35.0 120-121 22.611434443472497 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38216118055962767 1101 2 0.2745386544871131 1101 3 0.27094675860797324 1101 4 0.5028783460289361 1101 5 0.5644502765775812 1101 6 0.6281318673702927 1101 7 0.36199840069156863 1101 8 0.40694018796095577 1101 9 0.5897787865033379 1101 10-11 0.6125113013820709 1101 12-13 0.638174339559697 1101 14-15 0.7500509140900036 1101 16-17 0.540091652897992 1101 18-19 0.5225168638944169 1101 20-21 0.6460375816606287 1101 22-23 0.6007582021436804 1101 24-25 0.6136831961403928 1101 26-27 0.4697006111781121 1101 28-29 0.4967214753892648 1101 30-31 0.39394047362677753 1101 32-33 0.48263269399644315 1101 34-35 0.4775636819926561 1101 36-37 0.4887260473798207 1101 38-39 0.3852473898766817 1101 40-41 0.336385830951933 1101 42-43 0.41273817556891146 1101 44-45 0.47920421015647463 1101 46-47 0.3927666989818377 1101 48-49 0.41584822835991275 1101 50-51 0.10418333110357736 1101 52-53 0.15712264135910203 1101 54-55 0.20716937807205937 1101 56-57 0.15205319494508274 1101 58-59 0.23874894951079284 1101 60-61 0.10534551213213206 1101 62-63 0.13618496128984958 1101 64-65 0.20604791890640684 1101 66-67 0.1835893308546659 1101 68-69 0.00958027396358574 1101 70-71 -0.12736124776876778 1101 72-73 -0.10474190461551558 1101 74-75 -0.10501957374041737 1101 76-77 -0.09757291201265872 1101 78-79 0.1815850774293679 1101 80-81 0.21861791433740763 1101 82-83 0.2903651001131209 1101 84-85 0.24106451241165416 1101 86-87 0.19600429286763799 1101 88-89 0.29561649951897806 1101 90-91 0.29528049338604134 1101 92-93 0.2516632645869237 1101 94-95 0.30890869965318757 1101 96-97 0.34330927123133037 1101 98-99 0.34909658756645 1101 100-101 0.23098320981099008 1101 102-103 0.02557089438339588 1101 104-105 0.056816857174858626 1101 106-107 -0.005613152543887168 1101 108-109 -0.03356677904471894 1101 110-111 0.06266086577576857 1101 112-113 0.09437364998475672 1101 114-115 -0.006420730310974676 1101 116-117 -0.036367751706187335 1101 118-119 0.02829308684517784 1101 120-121 0.26323185210976874 1102 1 0.5372426590598707 1102 2 0.5411056282056528 1102 3 0.5749317251363273 1102 4 0.8126992361198688 1102 5 0.8920690390153538 1102 6 0.792615441229934 1102 7 0.6532310947569258 1102 8 0.7736859221410768 1102 9 0.7245706212411918 1102 10-11 0.8169968213026877 1102 12-13 0.7508435881210929 1102 14-15 0.818830312112496 1102 16-17 0.810133972192979 1102 18-19 0.7670018445814151 1102 20-21 0.7938717905205621 1102 22-23 0.8472254688558607 1102 24-25 0.8330585678049047 1102 26-27 0.8687814588654064 1102 28-29 0.8908662267784706 1102 30-31 0.8731509090925655 1102 32-33 0.7016332380500288 1102 34-35 0.7923849094811501 1102 36-37 0.7566661087018289 1102 38-39 0.6726281785796431 1102 40-41 0.7153841427591061 1102 42-43 0.8635969728791295 1102 44-45 0.8271384483579496 1102 46-47 0.9441552104741433 1102 48-49 0.8228619857457531 1102 50-51 0.6392095667769837 1102 52-53 0.7617145318880532 1102 54-55 0.8283079080911051 1102 56-57 0.8752886809753164 1102 58-59 0.8304810974138377 1102 60-61 0.9653444999394338 1102 62-63 0.8149113818691731 1102 64-65 0.8896239776569672 1102 66-67 0.7942726031049787 1102 68-69 0.7023516214390924 1102 70-71 0.6151648689117479 1102 72-73 0.552673943309756 1102 74-75 0.6377721682131074 1102 76-77 0.5240809777294331 1102 78-79 0.7233798999431862 1102 80-81 0.8577938840391042 1102 82-83 0.8392834547215813 1102 84-85 0.7592194387475217 1102 86-87 0.8043473966722203 1102 88-89 0.8337105677684811 1102 90-91 0.8559124932559037 1102 92-93 0.8950632560314844 1102 94-95 0.8219704289482728 1102 96-97 0.8951932600899823 1102 98-99 0.9614827384807683 1102 100-101 1.0002009384867065 1102 102-103 1.0142042866207213 1102 104-105 0.8452869580266089 1102 106-107 0.7734102682698101 1102 108-109 0.7674350055474157 1102 110-111 0.786931815905394 1102 112-113 0.6499148627964644 1102 114-115 0.6268733755342559 1102 116-117 0.6247607497726406 1102 118-119 0.5620380401909308 1102 120-121 0.6963604825226675 1103 1 0.531751693218979 1103 2 0.5522717844742786 1103 3 0.5682056578288233 1103 4 0.7014774222377582 1103 5 0.8532724466986679 1103 6 0.8496716494033905 1103 7 0.7403217641474527 1103 8 0.6504876402264017 1103 9 0.6845777886019917 1103 10-11 0.8161242341488517 1103 12-13 0.7766118599318759 1103 14-15 0.9210425193246721 1103 16-17 0.7970347585539272 1103 18-19 0.7183548871813557 1103 20-21 0.9254196087518736 1103 22-23 0.8563336917763849 1103 24-25 0.8625408837079576 1103 26-27 0.886430881590428 1103 28-29 0.8971768131719955 1103 30-31 0.8269111068909112 1103 32-33 0.8777810482814701 1103 34-35 0.708609185111051 1103 36-37 0.651005991194836 1103 38-39 0.6298367744696023 1103 40-41 0.7126456922423543 1103 42-43 0.7186196212839846 1103 44-45 0.6074365230890635 1103 46-47 0.6091105234104148 1103 48-49 0.7975568363352252 1103 50-51 0.5750038836842464 1103 52-53 0.5535959510905215 1103 54-55 0.5315216921876953 1103 56-57 0.5572004606498666 1103 58-59 0.6399282008927756 1103 60-61 0.5971215826609892 1103 62-63 0.782968740917859 1103 64-65 0.6827452949944615 1103 66-67 0.6571807304823878 1103 68-69 0.5372699691347904 1103 70-71 0.43592145961545015 1103 72-73 0.3777032680266501 1103 74-75 0.4690577306314232 1103 76-77 0.43651312113128427 1103 78-79 0.5330808610004176 1103 80-81 0.5863756620323812 1103 82-83 0.49171942245024525 1103 84-85 0.7048728197808778 1103 86-87 0.7647124036265751 1103 88-89 0.8080822529860576 1103 90-91 0.8045090368579473 1103 92-93 0.8028980366578864 1103 94-95 0.784686860318029 1103 96-97 0.8802351748419674 1103 98-99 0.7344866252455731 1103 100-101 0.7346587165853506 1103 102-103 0.8077318980326957 1103 104-105 0.8118142611510102 1103 106-107 0.6279563392130214 1103 108-109 0.6411981967920468 1103 110-111 0.7309396144752398 1103 112-113 0.7499671871318974 1103 114-115 0.7690149611534878 1103 116-117 0.6317656335940782 1103 118-119 0.5450961667065535 1103 120-121 0.5144418330433567 1104 1 0.5120455434473321 1104 2 0.5769971961363183 1104 3 0.6159249431591398 1104 4 0.8752636138485173 1104 5 1.0061845822647513 1104 6 0.8503244908613325 1104 7 0.588538170099298 1104 8 0.7814329643708717 1104 9 0.7062562659732663 1104 10-11 0.8914527828751275 1104 12-13 0.9285429440039934 1104 14-15 1.0856743604526073 1104 16-17 0.9441559296604964 1104 18-19 0.901031634292714 1104 20-21 0.8641024844954828 1104 22-23 0.8390912900048662 1104 24-25 0.9681749259391808 1104 26-27 1.0011721132829408 1104 28-29 0.9762254590627606 1104 30-31 0.8779370316495232 1104 32-33 0.7746204674314967 1104 34-35 0.8629663139276218 1104 36-37 0.8763607788309287 1104 38-39 0.7618825224497954 1104 40-41 0.90123073123263 1104 42-43 0.8983009405860827 1104 44-45 0.8711490693778003 1104 46-47 1.0204982869529786 1104 48-49 1.0267262987941521 1104 50-51 0.8734980028710169 1104 52-53 0.8979765939578712 1104 54-55 0.9374254852374619 1104 56-57 0.9085817673501282 1104 58-59 0.8613725857788523 1104 60-61 0.7798949523530396 1104 62-63 0.7818375154393493 1104 64-65 0.6700536122815492 1104 66-67 0.7261738541753928 1104 68-69 0.7522167410842897 1104 70-71 0.6992434257367037 1104 72-73 0.7149882859797927 1104 74-75 0.8012726192508914 1104 76-77 0.6249382727558839 1104 78-79 0.6285292728498213 1104 80-81 0.8830894207044508 1104 82-83 0.8834039757970302 1104 84-85 0.9088299234448911 1104 86-87 0.9502925570417595 1104 88-89 1.0464646018298716 1104 90-91 1.0867283859642747 1104 92-93 1.0011752407071413 1104 94-95 1.01419271250235 1104 96-97 0.9531062381427908 1104 98-99 1.0866030815280752 1104 100-101 0.9656324395888589 1104 102-103 0.9562616372105204 1104 104-105 1.0945783264464026 1104 106-107 1.1573225743243896 1104 108-109 1.1153618810672867 1104 110-111 1.0523969334951389 1104 112-113 1.0731610286437707 1104 114-115 0.995892193473793 1104 116-117 0.8806528763204788 1104 118-119 0.7095047278761868 1104 120-121 0.8561760907838334 1105 1 0.7119884671306913 1105 2 0.8091933348215754 1105 3 0.7494138774033168 1105 4 1.038413547047142 1105 5 1.04148101813756 1105 6 0.9567378473989052 1105 7 0.870293029018022 1105 8 0.9817733838308413 1105 9 0.9704941315228695 1105 10-11 0.9403354205483865 1105 12-13 1.1326396136179113 1105 14-15 1.3128967058305676 1105 16-17 1.1511726644025586 1105 18-19 1.0540182459894467 1105 20-21 1.1455388371829613 1105 22-23 1.1665483957090217 1105 24-25 1.2326777886731861 1105 26-27 1.362088086243034 1105 28-29 1.2999764262600166 1105 30-31 1.316674269104837 1105 32-33 1.2860034554616107 1105 34-35 1.219556972267256 1105 36-37 1.2410334266199428 1105 38-39 1.0578852267800372 1105 40-41 1.1975863404671045 1105 42-43 1.0867355971217023 1105 44-45 1.1195750115829313 1105 46-47 1.2471906379621274 1105 48-49 1.2263558123459646 1105 50-51 1.025278112963754 1105 52-53 1.049610191184236 1105 54-55 1.006006246951479 1105 56-57 1.0130488384772391 1105 58-59 1.1190335099905155 1105 60-61 1.0369638796495337 1105 62-63 1.047954567753358 1105 64-65 1.1344373961525633 1105 66-67 1.000403897211946 1105 68-69 0.9688510899853569 1105 70-71 0.7369804686908807 1105 72-73 0.7133464925264121 1105 74-75 0.6024372479023477 1105 76-77 0.684840376680647 1105 78-79 0.8558463207472151 1105 80-81 0.9432000400419369 1105 82-83 0.9529512977541685 1105 84-85 0.9636851899666574 1105 86-87 0.8340284397839 1105 88-89 0.8106599998819739 1105 90-91 0.8903365944219317 1105 92-93 0.9046869630104766 1105 94-95 0.8089481464309927 1105 96-97 0.6794546024221937 1105 98-99 0.735460153767832 1105 100-101 0.8282853331967388 1105 102-103 0.7942931497923631 1105 104-105 0.797344914038586 1105 106-107 0.8255320851752579 1105 108-109 1.0068400132787971 1105 110-111 0.960772882962214 1105 112-113 1.0363298174112892 1105 114-115 0.9752848403115344 1105 116-117 0.8507149892823236 1105 118-119 1.030190391103833 1105 120-121 1.1169872539600973 1106 1 0.48822158630422763 1106 2 0.6530495963983256 1106 3 0.6390688038118206 1106 4 0.8745928979993423 1106 5 0.8420120653803167 1106 6 0.834356437879471 1106 7 0.762590608075314 1106 8 0.6863662914632087 1106 9 0.8248679655281705 1106 10-11 0.84474977675292 1106 12-13 0.9864341356488708 1106 14-15 1.029245783075666 1106 16-17 1.0683113967508646 1106 18-19 0.8702412587923476 1106 20-21 0.980491498539358 1106 22-23 0.8112318862901233 1106 24-25 1.062838087691965 1106 26-27 0.9457646857103441 1106 28-29 1.1127504502875567 1106 30-31 1.049478281535734 1106 32-33 1.1006443189562418 1106 34-35 0.9656230742542462 1106 36-37 0.9816030066107047 1106 38-39 1.0343456404964542 1106 40-41 1.1291388185610387 1106 42-43 0.9837160823002904 1106 44-45 0.942207426825604 1106 46-47 1.0235686761261107 1106 48-49 1.042502335792939 1106 50-51 0.7861872058582335 1106 52-53 0.8444506071407432 1106 54-55 0.9869086071186999 1106 56-57 0.9183909986094534 1106 58-59 1.0053021574559082 1106 60-61 1.087506184503141 1106 62-63 1.0144806728789533 1106 64-65 1.003658744908975 1106 66-67 1.076780729017628 1106 68-69 0.9118626199627826 1106 70-71 0.8419542319578355 1106 72-73 0.8007256240045919 1106 74-75 0.7628807176924859 1106 76-77 1.1384976546558576 1106 78-79 1.1099675682839312 1106 80-81 0.7429835611251825 1106 82-83 0.9449783613626366 1106 84-85 1.0398155502923636 1106 86-87 0.9135993651761538 1106 88-89 0.8018912310007771 1106 90-91 0.8471374757290668 1106 92-93 0.7499771270200881 1106 94-95 0.805822586475351 1106 96-97 0.9643414704529398 1106 98-99 1.0094225634352583 1106 100-101 0.949888277626151 1106 102-103 0.8061096373200236 1106 104-105 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0.4600026646288313 2312 72-73 0.5136375854701356 2312 74-75 0.5997458029217206 2312 76-77 0.4242956297246785 2312 78-79 0.42224373519338343 2312 80-81 0.38367168116896266 2312 82-83 0.4065003075561009 2312 84-85 0.4519998644281209 2312 86-87 0.4358870018456251 2312 88-89 0.6566699744406286 2312 90-91 0.6581990608832236 2312 92-93 0.6848568520590632 2312 94-95 0.6136406433211832 2312 96-97 0.7020225867227232 2312 98-99 0.7688506069210987 2312 100-101 0.8674927977149878 2312 102-103 0.9713069600775412 2312 104-105 0.913592365393157 2312 106-107 0.9144840186178769 2312 108-109 1.0129882711242892 2312 110-111 1.0736853824538954 2312 112-113 1.0944063471977508 2312 114-115 1.035133673421747 2312 116-117 0.8349274588541036 2312 118-119 0.9896870257203858 2312 120-121 1.0417482515452292 2313 1 -0.5993690470976283 2313 2 -0.344249277111917 2313 3 -0.22139502496938235 2313 4 0.08785836218969223 2313 5 -2.525350604739618 2313 6 -8.036169373355637 2313 7 -3.172133235395865 2313 8 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70-71 -0.8104402192501254 2313 72-73 -0.48865823747469506 2313 74-75 -0.46450540661253115 2313 76-77 -0.3036657153026212 2313 78-79 -0.29708913706850026 2313 80-81 -0.4776742723219698 2313 82-83 -0.19786571375063033 2313 84-85 -0.1526528088289716 2313 86-87 -0.14261940217532043 2313 88-89 -0.3644287143076532 2313 90-91 -0.2322257988690204 2313 92-93 -0.7600674081602037 2313 94-95 -0.24921900836595512 2313 96-97 -0.25868161819598257 2313 98-99 -0.18058392677019697 2313 100-101 -0.09358577480655583 2313 102-103 -0.06613439224097917 2313 104-105 0.07352348953734733 2313 106-107 0.10644906249049413 2313 108-109 0.13373077361527663 2313 110-111 0.01302082469878485 2313 112-113 0.07734285087208193 2313 114-115 -0.04591784656519948 2313 116-117 -0.048095896547458494 2313 118-119 0.049658575339194044 2313 120-121 0.011107742044153213 2314 1 -0.3746183799365532 2314 2 -0.1737515976860955 2314 3 -0.27998736153493553 2314 4 -0.8282091883893514 2314 5 -0.08067550113512567 2314 6 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-3.2067604463277597 2315 6 -0.9811161921360352 2315 7 -0.7612870095447875 2315 8 -0.5025645060877721 2315 9 -0.24306067304846124 2315 10-11 -0.24255817811734914 2315 12-13 -0.17401506264108946 2315 14-15 -0.30751877734485333 2315 16-17 -0.31491930352165554 2315 18-19 -0.16222187430624047 2315 20-21 -0.27410498666286287 2315 22-23 -0.13174596591983345 2315 24-25 -0.03402729106705493 2315 26-27 -0.13461368778443017 2315 28-29 -0.11463381638160541 2315 30-31 -0.35108691100007405 2315 32-33 -0.3378347938236814 2315 34-35 -0.13081359583157592 2315 36-37 -0.315723617312635 2315 38-39 -0.4311333566241302 2315 40-41 -0.4062687598906578 2315 42-43 -0.46930948036097675 2315 44-45 -0.46614992431849345 2315 46-47 -0.505733247713188 2315 48-49 -0.4694620290581497 2315 50-51 -0.7328646523628919 2315 52-53 -0.8648648570192776 2315 54-55 -0.8298461086280255 2315 56-57 -0.6746407792810061 2315 58-59 -1.0402850006188658 2315 60-61 -1.0723226104667276 2315 62-63 -1.1008860139599435 2315 64-65 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4 -2.35934227685501 2316 5 -3.400059861664346 2316 6 -1.3629952231289124 2316 7 -1.1826473587089268 2316 8 -1.07281909530154 2316 9 -0.9671117607884554 2316 10-11 -0.6333429425277117 2316 12-13 -0.5834693201575902 2316 14-15 -5.15844876594366 2316 16-17 -1.7327710034701127 2316 18-19 -1.2194642775497613 2316 20-21 -0.8581606543988016 2316 22-23 -1.1771233962637737 2316 24-25 -0.8965006226218932 2316 26-27 -1.0234410642311786 2316 28-29 -0.6317462193142518 2316 30-31 -0.5615761319236086 2316 32-33 -1.0900768500430473 2316 34-35 -0.8299790298395138 2316 36-37 -1.1115473894074128 2316 38-39 -0.8000312440422661 2316 40-41 -0.7433550600998089 2316 42-43 -0.755692975313579 2316 44-45 -1.0573423155969053 2316 46-47 -1.0889413270139912 2316 48-49 -1.171810234907099 2316 50-51 -1.2129559486354893 2316 52-53 -1.2882214084377708 2316 54-55 -1.0390062450952797 2316 56-57 -1.2015098477886283 2316 58-59 -1.5619234480761577 2316 60-61 -1.2494479436641512 2316 62-63 -1.1967639091716578 2316 64-65 -1.019107094811858 2316 66-67 -0.7946180793492772 2316 68-69 -0.9945694786781374 2316 70-71 -0.9416828779613162 2316 72-73 -0.9445441746244754 2316 74-75 -0.8102786237496282 2316 76-77 -1.0281210015076354 2316 78-79 -0.8077683112183927 2316 80-81 -0.8615625570102239 2316 82-83 -0.7875080288346865 2316 84-85 -0.697815095147984 2316 86-87 -0.6694576140219155 2316 88-89 -0.6351438916360301 2316 90-91 -0.6847520894083736 2316 92-93 -0.5978056542724275 2316 94-95 -0.5662541295435659 2316 96-97 -0.650411742668755 2316 98-99 -0.3948661975148333 2316 100-101 -0.2597843928991743 2316 102-103 -0.22890891689540283 2316 104-105 -0.4307633544211491 2316 106-107 -0.4768077855708377 2316 108-109 -0.41918902758049015 2316 110-111 -0.4755765645853316 2316 112-113 -0.3958759350601646 2316 114-115 -0.19638453366513886 2316 116-117 -0.41346797988909145 2316 118-119 -0.3661369152911611 2316 120-121 -0.8409833147951034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42392.0 3 5996.0 4 5395.0 5 6104.0 6 7217.0 7 7540.0 8 9036.0 9 10755.0 10 13407.0 11 16045.0 12 18214.0 13 22005.0 14 27811.0 15 27633.0 16 19437.0 17 18824.0 18 19325.0 19 20889.0 20 23074.0 21 26247.0 22 29848.0 23 34042.0 24 39026.0 25 44731.0 26 50910.0 27 60450.0 28 70457.0 29 84257.0 30 100879.0 31 123030.0 32 151714.0 33 192310.0 34 251409.0 35 338864.0 36 483079.0 37 614576.0 38 285641.0 39 1221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.92363726225363 21.056031135264476 15.472067109577395 21.5482644929045 2 13.599678477704316 20.87793788401804 42.27074510532084 23.251638532956797 3 18.545265391185993 26.850367894222888 31.32258347299844 23.28178324159268 4 11.218568960710227 17.176331409372978 42.066480612238976 29.53861901767782 5 11.099638222249911 38.48611617567385 37.28311200939657 13.131133592679667 6 27.245171263347235 38.97903874565401 19.251995957230207 14.52379403376855 7 24.669473851349494 33.52810594424919 24.616037594696678 17.186382609704633 8 23.310318591441618 35.11744907018977 21.903756978003795 19.668475360364816 9 23.484481189968797 18.29399947487009 24.214900255848175 34.006619079312934 10-11 22.97164563276255 26.88082117322279 29.69720855382668 20.45032464018798 12-13 24.227958528128326 24.785402418927937 29.826453612792026 21.160185440151714 14-15 21.148309116139195 26.536445919359668 28.896025345666082 23.419219618835054 16-17 19.380262293029517 30.08944896172177 29.53049410302548 20.99979464222324 18-19 19.087064747282863 29.84732535290472 31.38902405461123 19.67658584520119 20-21 20.285056908145513 28.036149744902943 30.503676183122547 21.175117163828997 22-23 19.74044481303373 27.825227202011387 31.036865001561857 21.397462983393023 24-25 20.487687145270662 28.01277685852258 30.66265909055867 20.836876905648083 26-27 20.014191213782333 27.478943694954854 31.82162914401456 20.685235947248255 28-29 19.44417679905926 28.769052300690763 31.27018188298123 20.516589017268753 30-31 19.889093993245403 28.05925330476261 30.682997507187988 21.368655194804003 32-33 19.910053003770976 28.10742089440657 31.153259567003534 20.82926653481892 34-35 20.784106272572238 27.6395765944635 30.88997903680479 20.686338096159478 36-37 20.502693464358753 27.488915328843973 31.5030096237703 20.505381583026974 38-39 20.372412735712913 28.14829316683378 31.228805652009385 20.25048844544392 40-41 19.982109576325016 28.44888927104542 31.102197632519946 20.46680352010962 42-43 19.751984608763827 28.53681242709764 30.189189544148554 21.522013419989978 44-45 20.005160699290023 28.125246202000152 30.172944499580122 21.696648599129706 46-47 20.75223908670423 28.319652909499737 30.16440325423298 20.763704749563054 48-49 20.700978185791175 28.02779862698552 30.137521306397446 21.13370188082586 50-51 19.696555722347142 28.469679473542016 30.746550079195035 21.087214724915807 52-53 19.95391026778339 29.067068454230448 29.580169047597966 21.398852230388197 54-55 19.757179365209048 28.64874233693291 29.978949233063375 21.615129064794672 56-57 20.61700376106654 28.03238281961426 29.663869192623565 21.686744226695627 58-59 21.04511930029069 27.79239476684299 29.804850213282798 21.357635719583527 60-61 20.54318025312341 28.19946681917862 30.67496011531273 20.58239281238524 62-63 19.941692083994916 28.504361641014174 30.299440406481793 21.254505868509113 64-65 19.622108846393736 29.206726309850893 29.957500605007553 21.213664238747814 66-67 19.924922815707426 28.116046698405622 29.533428204102446 22.425602281784506 68-69 19.86829120343942 28.51671680348536 29.891913387767595 21.72307860530763 70-71 20.643157819988804 28.01554292974605 29.621986724829213 21.71931252543593 72-73 20.066863276648757 27.61308853756474 30.72610537372619 21.593942812060313 74-75 19.875441543524214 28.962933787065488 30.069280086087335 21.092344583322962 76-77 19.95243143899709 28.265504117226826 29.780085144394302 22.001979299381784 78-79 19.991302048222423 28.278967313127794 30.020728334991908 21.70900230365788 80-81 20.58638235562164 28.319162431502892 29.545458715792734 21.548996497082737 82-83 20.067757802158834 28.033036705072433 30.643569199164833 21.255636293603896 84-85 20.23723640865859 28.171155125374035 29.796344149695347 21.795264316272025 86-87 20.402573663346104 28.46948964352994 29.63464312286794 21.493293570256018 88-89 20.108921779773635 27.55653820892331 29.526829491820557 22.8077105194825 90-91 20.021909449294032 26.55739337742517 30.49302829774486 22.927668875535936 92-93 20.327186419490783 27.22452871717318 30.59976896186038 21.84851590147566 94-95 19.75402091766088 26.873732509432006 30.981356529155303 22.390890043751813 96-97 18.713153904351486 27.535702573084247 31.45193035162832 22.299213170935943 98-99 19.113854301041258 28.095952358467933 30.070080312264007 22.720113028226802 100-101 18.60919877406718 27.648833805433583 30.7949618194652 22.947005601034036 102-103 19.421417669980976 27.068988270600986 30.639511297443335 22.870082761974704 104-105 19.90749218321777 27.54380078350327 29.891393575004376 22.65731345827458 106-107 19.581619493695168 27.28835391366341 30.90064322058697 22.22938337205445 108-109 19.788652289903094 27.242207597435957 30.20055017053087 22.768589942130074 110-111 19.773222849494633 27.311609813550124 29.390268002417862 23.524899334537377 112-113 20.327955538224746 25.962337258273006 28.98849827870539 24.721208924796862 114-115 19.940802227874457 26.025895130312694 29.88910117020841 24.144201471604436 116-117 20.636288963233596 26.023684438908656 29.354344948301637 23.985681649556113 118-119 20.482178237184307 26.25652839247235 29.65787740005891 23.603415970284434 120-121 20.08112192957468 27.73268712764064 29.174888707463875 23.0113022353208 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8433.0 1 8846.5 2 5366.5 3 1514.5 4 1594.0 5 1588.0 6 1529.5 7 1534.0 8 1664.5 9 1748.5 10 1734.5 11 1778.5 12 1904.5 13 2095.0 14 2325.0 15 2583.5 16 2824.0 17 3116.5 18 3471.5 19 3916.0 20 4410.5 21 4929.5 22 5693.0 23 6721.0 24 8125.5 25 10329.5 26 13721.5 27 18800.5 28 27760.0 29 40938.0 30 56320.0 31 72829.0 32 89663.5 33 104070.0 34 115403.0 35 124586.0 36 130477.5 37 135530.0 38 137176.0 39 132293.5 40 127208.5 41 123835.0 42 121871.5 43 123953.5 44 124235.0 45 123932.5 46 128063.5 47 144716.5 48 179842.0 49 170980.0 50 126624.5 51 100882.5 52 81470.5 53 67935.0 54 57563.5 55 47618.5 56 37962.5 57 29333.5 58 22822.0 59 18283.5 60 14597.5 61 11064.0 62 8006.0 63 5652.5 64 3768.0 65 2420.0 66 1483.5 67 875.5 68 530.5 69 326.0 70 206.0 71 144.0 72 100.5 73 61.0 74 50.0 75 45.5 76 35.0 77 27.0 78 23.5 79 15.0 80 10.0 81 9.5 82 7.0 83 7.0 84 7.0 85 5.0 86 3.0 87 3.5 88 4.5 89 6.0 90 7.0 91 4.0 92 2.5 93 4.0 94 3.5 95 2.0 96 2.0 97 3.0 98 2.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4203354329421664 2 0.8888882162607187 3 0.9046277154419622 4 0.8814422224172844 5 0.8900081421639995 6 0.9019035713529009 7 0.8735422045590064 8 0.8625850916674486 9 0.8599214841136997 10-11 0.8501296995269071 12-13 0.8512647595640158 14-15 0.8511890888948752 16-17 0.8783094567148639 18-19 0.9089863459844604 20-21 0.9002388166318077 22-23 0.8729671074735379 24-25 0.8624488844629956 26-27 0.8633720666265108 28-29 0.8883585215767346 30-31 0.8952142842008723 32-33 0.8870418519336882 34-35 0.8995275123418861 36-37 0.9118315631441465 38-39 0.8967579658513405 40-41 0.9071853840589141 42-43 0.8995426464757144 44-45 0.8789904927371293 46-47 0.8715444988936949 48-49 0.8763268851833803 50-51 0.9053390197318836 52-53 0.8805341743875973 54-55 0.894805662587513 56-57 0.9478205333874126 58-59 0.9141622197536768 60-61 0.9263300633514842 62-63 0.8913702142085302 64-65 0.8789148220679885 66-67 0.8826226848558777 68-69 0.9004506945054013 70-71 0.8969244413234498 72-73 0.9018581689514164 74-75 0.9051876783936026 76-77 0.920442885292346 78-79 0.9959773472284861 80-81 1.0274260773233166 82-83 0.986140160240209 84-85 0.9402231982056971 86-87 0.9202007391510962 88-89 0.9183392406902375 90-91 0.9314454005853883 92-93 0.9172647171884412 94-95 0.9109840516497719 96-97 0.9220168352104704 98-99 0.9591105972231891 100-101 0.9437645855214769 102-103 0.9173101195899255 104-105 0.9051574101259462 106-107 0.9065951528396174 108-109 0.9049001298508682 110-111 0.9028116193825878 112-113 0.9152064749878169 114-115 0.9188235329727373 116-117 0.9487891179524123 118-119 0.9432197567036646 120-121 0.9178095460062534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 3303790.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.99270644380457 #Duplication Level Percentage of deduplicated Percentage of total 1 77.15447320965671 41.657788228353795 2 13.7830223912345 14.883653637566194 3 4.113836996769569 6.663515799727258 4 1.6785413904076298 3.6251597018421893 5 0.8361868615268856 2.25739958732937 6 0.4986086458026535 1.6152738145899361 7 0.33718042200130877 1.2743698480600367 8 0.23800944101848107 1.0280619103811894 9 0.17917505070384115 0.8706731323235668 >10 1.0307928718180193 10.42530522146711 >50 0.08895983153370775 3.3350042794419283 >100 0.05546079428174816 5.339906949055609 >500 0.0027906194955396235 1.034447700095089 >1k 0.002391959566861735 2.865679347806652 >5k 3.417085095516764E-4 1.1620893395745326 >10k+ 2.2780567303445093E-4 1.961671502385619 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20047 0.6067879617045878 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18719 0.5665917022571046 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12565 0.38032078310062084 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12464 0.37726368806734084 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8059 0.24393196904161585 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6645 0.2011326385756964 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6258 0.18941881899273258 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6121 0.1852720663238281 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5516 0.16695976439180457 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5193 0.15718311393883994 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4799 0.14525741648228246 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4322 0.1308194528102573 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4106 0.12428150699651007 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3934 0.11907536495963728 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3536 0.10702859443245485 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3478 0.10527303490839308 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.026826765623723E-5 0.0 4 0.0 0.0 0.0 9.08048029687117E-5 0.0 5 0.0 0.0 0.0 9.08048029687117E-5 0.0 6 0.0 0.0 0.0 9.08048029687117E-5 0.0 7 0.0 0.0 0.0 9.08048029687117E-5 0.0 8 0.0 0.0 0.0 9.08048029687117E-5 0.0 9 0.0 0.0 0.0 1.2107307062494892E-4 0.0 10-11 0.0 0.0 0.0 1.5134133828118614E-4 0.0 12-13 0.0 1.5134133828118615E-5 0.0 1.6647547210930477E-4 0.0 14-15 0.0 4.540240148435585E-5 0.0 2.724144089061351E-4 0.0 16-17 0.0 7.567066914059307E-5 0.0 3.026826765623723E-4 0.0 18-19 0.0 1.2107307062494892E-4 0.0 4.691581486716771E-4 0.0 20-21 0.0 1.2107307062494892E-4 0.0 6.053653531247446E-4 0.0 22-23 0.0 1.2107307062494892E-4 0.0 8.626456282027611E-4 0.0 24-25 0.0 1.2107307062494892E-4 0.0 0.0012864013753900823 0.0 26-27 3.026826765623723E-5 1.2107307062494892E-4 0.0 0.0020279739329678945 0.0 28-29 3.026826765623723E-5 1.2107307062494892E-4 0.0 0.00458564254991994 0.0 30-31 4.540240148435585E-5 1.2107307062494892E-4 0.0 0.009549638445542847 0.0 32-33 6.053653531247446E-5 1.2107307062494892E-4 0.0 0.01737398563468017 0.0 34-35 6.053653531247446E-5 1.2107307062494892E-4 0.0 0.024789711210458292 0.0 36-37 6.053653531247446E-5 1.5134133828118614E-4 0.0 0.03396099631029817 0.0 38-39 6.053653531247446E-5 1.96743739765542E-4 0.0 0.043722512629434676 0.0 40-41 6.053653531247446E-5 2.1187787359366062E-4 0.0 0.06395684955762927 0.0 42-43 6.053653531247446E-5 2.1187787359366062E-4 0.0 0.08744502525886935 0.0 44-45 6.053653531247446E-5 2.1187787359366062E-4 0.0 0.1359347900441614 0.0 46-47 6.053653531247446E-5 2.1187787359366062E-4 0.0 0.22672445887904497 0.0 48-49 6.053653531247446E-5 2.1187787359366062E-4 0.0 0.2876242134033943 0.0 50-51 6.053653531247446E-5 2.724144089061351E-4 0.0 0.3616906643582068 0.0 52-53 6.053653531247446E-5 2.724144089061351E-4 0.0 0.45807996270949425 0.0 54-55 6.053653531247446E-5 2.724144089061351E-4 0.0 0.5625962909264814 0.0 56-57 6.053653531247446E-5 2.724144089061351E-4 0.0 0.6640403899763605 0.0 58-59 6.053653531247446E-5 2.724144089061351E-4 0.0 0.8688052206708053 0.0 60-61 6.053653531247446E-5 2.724144089061351E-4 0.0 1.0572705892323664 0.0 62-63 6.053653531247446E-5 2.724144089061351E-4 0.0 1.2673929033019653 0.0 64-65 6.053653531247446E-5 2.724144089061351E-4 0.0 1.3894799608933983 0.0 66-67 6.053653531247446E-5 2.724144089061351E-4 0.0 1.7527597093035574 0.0 68-69 6.053653531247446E-5 2.724144089061351E-4 0.0 2.1417674852215187 0.0 70-71 6.053653531247446E-5 2.724144089061351E-4 0.0 2.2923369826774707 0.0 72-73 6.053653531247446E-5 2.724144089061351E-4 0.0 2.717545606712291 0.0 74-75 6.053653531247446E-5 2.724144089061351E-4 0.0 2.9359462919858705 0.0 76-77 6.053653531247446E-5 2.724144089061351E-4 0.0 3.077616918750889 0.0 78-79 6.053653531247446E-5 2.724144089061351E-4 0.0 3.24846615553652 0.0 80-81 6.053653531247446E-5 2.724144089061351E-4 0.0 3.4244307295560557 0.0 82-83 6.053653531247446E-5 2.875485427342537E-4 0.0 3.7478774377306063 0.0 84-85 6.053653531247446E-5 3.026826765623723E-4 0.0 4.386885970355259 0.0 86-87 6.053653531247446E-5 3.026826765623723E-4 0.0 5.526259235605169 0.0 88-89 6.053653531247446E-5 3.480850780467282E-4 0.0 6.780878929956202 0.0 90-91 6.053653531247446E-5 3.632192118748468E-4 0.0 8.08563800967979 0.0 92-93 6.053653531247446E-5 3.632192118748468E-4 0.0 9.343980700952542 0.0 94-95 6.053653531247446E-5 3.783533457029654E-4 0.0 10.45907881554215 0.0 96-97 6.053653531247446E-5 3.93487479531084E-4 0.0 11.588418150064017 0.0 98-99 6.053653531247446E-5 4.086216133592026E-4 0.0 12.852996104473952 0.0 100-101 6.053653531247446E-5 4.2375574718732124E-4 0.0 13.87647217286813 0.0 102-103 6.053653531247446E-5 4.2375574718732124E-4 0.0 14.54662675291105 0.0 104-105 6.053653531247446E-5 4.5402401484355843E-4 0.0 15.23061393127287 0.0 106-107 6.053653531247446E-5 4.5402401484355843E-4 0.0 16.350327956680054 0.0 108-109 6.053653531247446E-5 4.842922824997957E-4 0.0 17.555988728097127 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2670 0.0 34.470314 2 ACCTGGG 2850 0.0 30.642017 3 CATGGGT 3645 0.0 25.692247 4 GTACCCG 295 9.094947E-12 25.393116 1 GTACATG 13035 0.0 25.330177 1 TAACGCA 555 0.0 24.8445 4 TACATGG 12705 0.0 24.222319 2 ACATGGG 12805 0.0 23.01741 3 TATAACG 550 0.0 23.008938 2 CTGCGCG 125 0.0020095634 22.96888 9 GAGTACT 8865 0.0 22.749763 12-13 GTACCGG 285 4.642061E-9 22.240395 1 GTACACG 325 9.113137E-10 21.276123 1 AGTACTT 9715 0.0 21.173307 12-13 GTACCTG 4575 0.0 20.907959 1 ATAACGC 695 0.0 20.666828 3 TCACGCA 475 0.0 20.562092 4 GTACTAG 1080 0.0 20.274704 1 TCTCGGG 1405 0.0 20.051662 114-115 GTACTTT 10030 0.0 20.051434 14-15 >>END_MODULE