FastQCFastQC Report
Wed 25 May 2016
SRR1003807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303790
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT351631.0643230955962697No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT119620.36206901770390976No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT93800.28391635061550524No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC86120.260670321055515No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC84910.2570078606691103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79040.23924038755489904No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC76270.23085607741412137No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG75640.2289491765517784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69930.21166599572006695No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA61170.18515099325320314No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA58930.17837090129820599No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58310.17649426870351928No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT54110.16378159628789965No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC43780.1325144757990066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39120.11840946307120005No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC37030.11208339513104645No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT33060.10006689287152028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG27750.034.4155732
ACCTGGG30200.030.677273
GTACCTG40300.024.1802881
TAGACCG1201.71378E-423.7458745
ACGCGAT1452.3394721E-522.9267028
TCACGCA3750.022.7960384
TCGATCG1050.002198920822.61589
TAACGCA4950.022.066874
GTACACG3900.021.939191
ATAACGC5850.020.3036083
ACGTGCG2601.4950274E-820.0932679
TATAACG6050.019.63392
GAGTACT103350.019.32354412-13
GTACCCG3203.929017E-1019.3110561
AGTACTT106300.019.27874812-13
GTATAAC10600.018.8345871
TACACGG4350.018.5687052
CCTGGGG23050.018.5434154
GTACTTT108800.018.07472614-15
CTAACGC1851.51987E-417.9769253
Produced by FastQC (version 0.11.5)