##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003807_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3303790 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.70652523314133 31.0 31.0 34.0 30.0 34.0 2 31.943481274536214 31.0 31.0 34.0 30.0 34.0 3 31.969503509605634 31.0 31.0 34.0 30.0 34.0 4 35.720081784859204 37.0 35.0 37.0 35.0 37.0 5 35.5882840616383 37.0 35.0 37.0 33.0 37.0 6 35.6246380671895 37.0 35.0 37.0 33.0 37.0 7 35.553250357922266 37.0 35.0 37.0 33.0 37.0 8 35.54970715451043 37.0 35.0 37.0 33.0 37.0 9 37.129928657693135 39.0 37.0 39.0 33.0 39.0 10-11 37.052298874928496 39.0 37.0 39.0 33.0 39.0 12-13 37.00593939082084 39.0 37.0 39.0 33.0 39.0 14-15 38.14245911513746 40.0 37.0 41.0 33.0 41.0 16-17 38.15158393844645 40.0 37.0 41.0 33.0 41.0 18-19 38.17080322901879 40.0 37.0 41.0 33.0 41.0 20-21 38.12269333099259 40.0 37.0 41.0 33.0 41.0 22-23 38.04228552662246 40.0 37.0 41.0 33.0 41.0 24-25 37.95581695567817 40.0 37.0 41.0 33.0 41.0 26-27 37.76185320495552 40.0 37.0 41.0 32.5 41.0 28-29 37.60781572073286 39.0 37.0 41.0 32.0 41.0 30-31 37.41506951107667 39.0 36.0 41.0 32.0 41.0 32-33 37.19035652992472 39.0 36.0 40.5 31.0 41.0 34-35 37.19668123579284 39.0 36.0 41.0 31.0 41.0 36-37 37.05522944860297 39.0 36.0 41.0 31.0 41.0 38-39 36.83287996513096 39.0 35.0 40.0 30.0 41.0 40-41 36.9624927734511 39.0 36.0 41.0 31.0 41.0 42-43 36.84103378241353 39.0 36.0 41.0 30.0 41.0 44-45 36.70746400346269 39.0 35.5 41.0 30.0 41.0 46-47 36.470123403727236 39.0 35.0 41.0 30.0 41.0 48-49 36.251244479824685 39.0 35.0 41.0 30.0 41.0 50-51 36.03962948613562 39.0 35.0 40.5 29.0 41.0 52-53 35.88530551275959 39.0 35.0 40.0 28.5 41.0 54-55 35.699822476610194 39.0 35.0 40.0 28.0 41.0 56-57 35.47826238955866 38.0 34.5 40.0 28.0 41.0 58-59 35.24262574195091 38.0 34.0 40.0 27.0 41.0 60-61 34.855523504823246 38.0 34.0 40.0 26.0 41.0 62-63 34.658947905284535 38.0 34.0 40.0 26.0 41.0 64-65 34.254752723387384 37.0 33.0 40.0 26.0 41.0 66-67 33.944653564542534 37.0 33.0 39.5 25.5 41.0 68-69 33.69033246665194 36.0 33.0 39.0 25.0 41.0 70-71 33.28272877513402 36.0 32.0 39.0 24.5 40.5 72-73 32.941928663746786 35.0 32.0 38.5 24.0 40.0 74-75 32.56857654390866 35.0 32.0 38.0 23.0 40.0 76-77 31.562864013753902 34.0 30.5 36.5 22.0 39.0 78-79 31.954652686762778 35.0 31.0 37.0 23.0 39.0 80-81 31.755821041894308 35.0 31.0 36.0 22.0 39.0 82-83 31.43615166218192 35.0 31.0 36.0 21.5 37.5 84-85 31.07590494553225 34.0 31.0 35.5 20.0 37.0 86-87 30.73115104168243 34.0 31.0 35.0 19.5 37.0 88-89 30.426940120286098 34.0 31.0 35.0 18.5 36.0 90-91 30.1331719025725 34.0 30.5 35.0 17.0 36.0 92-93 29.866683263766767 34.0 30.0 35.0 11.5 36.0 94-95 29.549163385081982 34.0 30.0 35.0 4.5 35.0 96-97 29.19028101059692 34.0 30.0 35.0 2.0 35.0 98-99 28.84487649033383 34.0 29.5 35.0 2.0 35.0 100-101 27.88478520123858 33.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0399558859741056 1101 2 0.034542768749865616 1101 3 0.04507234942894556 1101 4 0.084830077773276 1101 5 0.0650197940702597 1101 6 0.13022949684874874 1101 7 0.09141593507602153 1101 8 0.026907066568021776 1101 9 0.15000753601363215 1101 10-11 0.13008770362092292 1101 12-13 0.19604726479880696 1101 14-15 0.12855944555303012 1101 16-17 0.14931747319955235 1101 18-19 -0.014199316821006391 1101 20-21 -0.05233849258966217 1101 22-23 0.04291339956998286 1101 24-25 0.03222026260592514 1101 26-27 -0.0051960065432012925 1101 28-29 0.0410321737381949 1101 30-31 0.042662108857960845 1101 32-33 0.14719199185341836 1101 34-35 0.1087143656327001 1101 36-37 0.08447793331230713 1101 38-39 0.02910360189536476 1101 40-41 0.15457501038478227 1101 42-43 -0.008683212440892873 1101 44-45 -0.1075558671953587 1101 46-47 -0.045928734877300315 1101 48-49 -0.016580684242271104 1101 50-51 -0.08451516072568666 1101 52-53 0.07148117314326186 1101 54-55 0.13912167765447947 1101 56-57 0.14808772789740487 1101 58-59 -0.05678791589884469 1101 60-61 0.04002809771856164 1101 62-63 -0.07005620074469476 1101 64-65 -0.10518629526660561 1101 66-67 -0.28459344210923376 1101 68-69 -0.29711179086149997 1101 70-71 -0.1062127840624143 1101 72-73 -0.18629719592158267 1101 74-75 -0.15709706461946382 1101 76-77 -0.1589065397887559 1101 78-79 -0.189193502594577 1101 80-81 -0.1856312425402571 1101 82-83 -0.20487151807730797 1101 84-85 -0.3303302325450481 1101 86-87 -0.40390456320049495 1101 88-89 -0.38081084420218403 1101 90-91 -0.29832961478526343 1101 92-93 -0.2209900016667241 1101 94-95 -0.18422127203778516 1101 96-97 -0.09198439887817145 1101 98-99 -0.2565889375882193 1101 100-101 -0.14508098111607737 1102 1 -0.0710985236012398 1102 2 0.062099413885345456 1102 3 0.007951386978284347 1102 4 0.04893007277256345 1102 5 -0.015253262986682614 1102 6 -0.026086495253736075 1102 7 -0.08745659070024914 1102 8 -0.10867539309639795 1102 9 -0.07205820469754087 1102 10-11 0.03352549865551424 1102 12-13 -0.04263447771503337 1102 14-15 0.07596511431417241 1102 16-17 0.05235332831862394 1102 18-19 -0.04710778025825135 1102 20-21 -0.12064432902534605 1102 22-23 -0.063556319299515 1102 24-25 -0.061145175712674416 1102 26-27 -0.10769332091881267 1102 28-29 0.043777219265784595 1102 30-31 -0.10260253133463237 1102 32-33 -0.12850248982860535 1102 34-35 -0.23928140243779694 1102 36-37 -0.2981763221446414 1102 38-39 -0.18775025013049174 1102 40-41 -0.15858061785852584 1102 42-43 -0.11875615659014471 1102 44-45 -0.045712248558238855 1102 46-47 -0.100517251430567 1102 48-49 -0.06281627631174302 1102 50-51 -0.12180105636259242 1102 52-53 -0.07190970555448217 1102 54-55 -0.10421374131798444 1102 56-57 -0.04640103344985391 1102 58-59 -0.2713067960040192 1102 60-61 -0.19983574127531512 1102 62-63 -0.2724549016435631 1102 64-65 -0.2680845524274176 1102 66-67 -0.42767215274442094 1102 68-69 -0.3618662166439748 1102 70-71 -0.34001634517324675 1102 72-73 -0.3517279682667933 1102 74-75 -0.3373095407380404 1102 76-77 -0.27579190233750595 1102 78-79 -0.25191232148619136 1102 80-81 -0.18047982935656748 1102 82-83 -0.3229776121401322 1102 84-85 -0.40107813450454444 1102 86-87 -0.423165822187471 1102 88-89 -0.4247129705901109 1102 90-91 -0.4063400656749394 1102 92-93 -0.26227609190972956 1102 94-95 -0.2772461496859826 1102 96-97 -0.22128139790754986 1102 98-99 -0.2529313370753741 1102 100-101 -0.32538337971154263 1103 1 -0.09001300664558443 1103 2 -0.10751354537939406 1103 3 -0.0662584391351686 1103 4 -0.01974586138267398 1103 5 0.017573103145132052 1103 6 -0.03235956194433953 1103 7 -0.0615402725643861 1103 8 0.023811271352414565 1103 9 -0.07461263387611439 1103 10-11 -0.04404390588444329 1103 12-13 0.04015711663576127 1103 14-15 -0.04102908350563439 1103 16-17 -0.12171337821730077 1103 18-19 -0.09376814459042038 1103 20-21 -0.03591947405546847 1103 22-23 -0.11150259726368716 1103 24-25 -0.0716612614621468 1103 26-27 -0.03971452967275013 1103 28-29 -0.13051448353849793 1103 30-31 0.01247820228571328 1103 32-33 0.007317290070979254 1103 34-35 -0.1007604478259907 1103 36-37 -0.1597418295134574 1103 38-39 -0.2800912389466177 1103 40-41 -0.16460437974084385 1103 42-43 0.06214840401714383 1103 44-45 0.0724360358116769 1103 46-47 0.15381706692708264 1103 48-49 0.058079970612297416 1103 50-51 -0.016823935657932054 1103 52-53 0.020437096173360203 1103 54-55 -0.022943259950359618 1103 56-57 0.011794424121426061 1103 58-59 0.031801997963945894 1103 60-61 0.1162559674475645 1103 62-63 -0.11833473420568197 1103 64-65 -0.08938109352543933 1103 66-67 -0.1873900403501878 1103 68-69 -0.18287446984042077 1103 70-71 -0.11405830415213103 1103 72-73 0.011715394135975998 1103 74-75 -0.03729280930821943 1103 76-77 -0.1031185060263553 1103 78-79 -0.009220452310692906 1103 80-81 -0.1027022168877032 1103 82-83 -0.18647292490080147 1103 84-85 -0.33754618007517223 1103 86-87 -0.3579945047036297 1103 88-89 -0.27949379373743355 1103 90-91 -0.15456107078997405 1103 92-93 -0.3047223391427032 1103 94-95 -0.4202421520287061 1103 96-97 -0.293902754431393 1103 98-99 -0.2674939410777135 1103 100-101 -0.237233866093721 1104 1 -0.04365666205674401 1104 2 -0.03393417210208227 1104 3 -0.06333777879907032 1104 4 -0.10970659631754387 1104 5 -0.03677403111348099 1104 6 -0.09929319322285579 1104 7 -0.006012195652807861 1104 8 -0.07850948545289071 1104 9 -0.17865495967876655 1104 10-11 -0.10586229173010508 1104 12-13 -0.21557601486373557 1104 14-15 -0.1309962088228218 1104 16-17 -0.08403197762954306 1104 18-19 -0.22419738145863022 1104 20-21 -0.194695168824218 1104 22-23 -0.12558495497735578 1104 24-25 -0.05411173791627277 1104 26-27 -0.06145167075373337 1104 28-29 -0.03612994253793289 1104 30-31 -0.1260863747003711 1104 32-33 -0.07602786546964779 1104 34-35 0.043005261331352074 1104 36-37 -0.030458004772505376 1104 38-39 -0.1285286243191308 1104 40-41 -0.1552544835589984 1104 42-43 -0.0475313892616569 1104 44-45 0.06941261812785626 1104 46-47 -0.03267742239062699 1104 48-49 -0.03201496235132595 1104 50-51 -0.0569058194608445 1104 52-53 4.6548992009576295E-4 1104 54-55 -0.10507002519762665 1104 56-57 -0.14900740526610434 1104 58-59 -0.05203214963190561 1104 60-61 -0.16915742255056898 1104 62-63 -0.2133259781038248 1104 64-65 -0.1950975716885992 1104 66-67 -0.17857489136098081 1104 68-69 -0.28820458548845096 1104 70-71 -0.13690264287409804 1104 72-73 -0.06634798260850516 1104 74-75 -0.1868997113301063 1104 76-77 -0.17844564886852865 1104 78-79 -0.13200717534564177 1104 80-81 -0.10916915598915011 1104 82-83 -0.13841277657747142 1104 84-85 -0.1905631690343803 1104 86-87 -0.13858903520096177 1104 88-89 -0.08961572478827762 1104 90-91 -0.08972770256515261 1104 92-93 -0.2693841639444905 1104 94-95 -0.3137147343489737 1104 96-97 -0.16374983769475548 1104 98-99 -0.23477735632296515 1104 100-101 -0.1579246204118938 1105 1 0.021100326182306617 1105 2 0.05295772518432784 1105 3 0.035507020333913175 1105 4 0.07467793498143038 1105 5 -0.02453065158400136 1105 6 0.10850366944829659 1105 7 0.05305754532093232 1105 8 0.0803198045500011 1105 9 0.1153016606218884 1105 10-11 0.004671261414991079 1105 12-13 0.037629219510129985 1105 14-15 0.11015207833560225 1105 16-17 0.1491150083247632 1105 18-19 0.08541275842081575 1105 20-21 0.08231904334849105 1105 22-23 0.15476932587380077 1105 24-25 0.1287448128226174 1105 26-27 0.0784052647406952 1105 28-29 0.1009738250935115 1105 30-31 0.2544402392556364 1105 32-33 0.24284995638939222 1105 34-35 0.2262745961459558 1105 36-37 0.20671702331974728 1105 38-39 0.33205644190044126 1105 40-41 0.21136147691565554 1105 42-43 0.3231143120508406 1105 44-45 0.3265630830195505 1105 46-47 0.3498277522739457 1105 48-49 0.29989995656548984 1105 50-51 0.277884573711745 1105 52-53 0.37677380734934474 1105 54-55 0.358908618747229 1105 56-57 0.34039355670765303 1105 58-59 0.26864714347112084 1105 60-61 0.2949498979117138 1105 62-63 0.3015966758429798 1105 64-65 0.15372790473301734 1105 66-67 0.26467961380348726 1105 68-69 0.2610441220132671 1105 70-71 0.3505995972863971 1105 72-73 0.22882405747062506 1105 74-75 0.14964841156402997 1105 76-77 0.0983264985684329 1105 78-79 0.17677612235745954 1105 80-81 0.3198294349392832 1105 82-83 0.26026571983529934 1105 84-85 0.21761488189434175 1105 86-87 0.31194100163534344 1105 88-89 0.1998486836267439 1105 90-91 0.11955934459330209 1105 92-93 0.186196532369582 1105 94-95 0.3044561735466722 1105 96-97 0.32873851134730714 1105 98-99 0.2911592431006653 1105 100-101 0.4023868701887743 1106 1 -0.10131592213281948 1106 2 -0.05622362863463337 1106 3 -0.03137046784507547 1106 4 0.00777205578386031 1106 5 0.061172785819572084 1106 6 -0.027429823602716397 1106 7 -0.006383783911196872 1106 8 0.029016402006021735 1106 9 5.4984336270536E-4 1106 10-11 -0.037774247328655974 1106 12-13 -0.09041437827877274 1106 14-15 -0.1700320722586781 1106 16-17 -0.003157879906034111 1106 18-19 -0.07837861563896809 1106 20-21 -0.08931074775289005 1106 22-23 -0.04436621440884636 1106 24-25 -0.1768690587170667 1106 26-27 -0.11640002123018434 1106 28-29 0.025142922416158342 1106 30-31 -0.0707584432763042 1106 32-33 -0.0517792428684416 1106 34-35 -0.0770963329277734 1106 36-37 -0.01432957154156611 1106 38-39 0.17056541632221212 1106 40-41 -0.004555391270052667 1106 42-43 0.06092533539938927 1106 44-45 0.10041042066477956 1106 46-47 -0.00404019121898358 1106 48-49 0.0614606389358201 1106 50-51 0.20414914659799166 1106 52-53 0.21259811457469624 1106 54-55 0.16767685724676795 1106 56-57 0.16383989981446234 1106 58-59 0.11430753468271604 1106 60-61 0.09437312344964965 1106 62-63 -0.022120941886413448 1106 64-65 -0.026233298368111946 1106 66-67 -0.01961260278316246 1106 68-69 0.009807474761679202 1106 70-71 0.09984861324641514 1106 72-73 0.012511430965496118 1106 74-75 -0.005278100936287444 1106 76-77 0.044566700937114234 1106 78-79 0.13913628749126872 1106 80-81 0.049545742105980395 1106 82-83 -0.031754885596413374 1106 84-85 0.04750640220426661 1106 86-87 0.05474894825024634 1106 88-89 0.04582527590790164 1106 90-91 0.026741669114709765 1106 92-93 0.1053376115007616 1106 94-95 0.11186715496386412 1106 96-97 0.21824877875638649 1106 98-99 0.11937803323352725 1106 100-101 0.3311648801905278 1107 1 -0.0619518594745756 1107 2 -0.11468569563725595 1107 3 -0.09556137957105904 1107 4 -0.04743892948925321 1107 5 -0.16506026795784123 1107 6 -0.018140285908941678 1107 7 -0.09620411117148109 1107 8 -0.01231206048878164 1107 9 -0.059988266867186724 1107 10-11 -0.040732797182506886 1107 12-13 -0.05342647548950907 1107 14-15 0.03699493518333696 1107 16-17 -0.0783386265176702 1107 18-19 -0.0486491702457954 1107 20-21 -0.04590656943078386 1107 22-23 -0.054819004205839406 1107 24-25 -0.0771012835177487 1107 26-27 -0.11841860576224406 1107 28-29 -0.09281552266970294 1107 30-31 -0.08821502785725244 1107 32-33 -0.12103083054004315 1107 34-35 -0.1477068487529749 1107 36-37 -0.031499790279674755 1107 38-39 0.06909718023103295 1107 40-41 0.09090325462112503 1107 42-43 0.0934807336574437 1107 44-45 0.12055414052215241 1107 46-47 0.025924163654650556 1107 48-49 0.015734703706883124 1107 50-51 0.10650892555498359 1107 52-53 0.01160203289757078 1107 54-55 -0.02757324577509479 1107 56-57 -0.08487587379238448 1107 58-59 -0.011778942126888126 1107 60-61 0.06996703791357817 1107 62-63 -0.0925397650275741 1107 64-65 -0.05204540308721306 1107 66-67 0.003233094482737897 1107 68-69 0.017745669067892322 1107 70-71 0.1256281962348922 1107 72-73 0.1440175232241927 1107 74-75 0.23197174365785855 1107 76-77 0.1657951623842422 1107 78-79 0.15047267524808206 1107 80-81 0.09072070858021775 1107 82-83 0.1022071429912792 1107 84-85 0.18301757832973564 1107 86-87 0.2453593571247943 1107 88-89 0.1381009392639001 1107 90-91 0.1454178295075188 1107 92-93 0.0308835836953385 1107 94-95 0.07920878941437337 1107 96-97 0.2251286526506462 1107 98-99 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-0.7298348036098901 2316 62-63 -0.7086744499838531 2316 64-65 -0.6412983676133948 2316 66-67 -0.6798750730381116 2316 68-69 -0.6731161161798198 2316 70-71 -0.7471264264161093 2316 72-73 -0.6050403604055958 2316 74-75 -0.5089242718179676 2316 76-77 -0.5931518027448881 2316 78-79 -0.5068560479958109 2316 80-81 -0.5299220032075382 2316 82-83 -0.6042168344984198 2316 84-85 -0.6257799043353209 2316 86-87 -0.41596550972276347 2316 88-89 -0.6029222619219112 2316 90-91 -0.7099044999066102 2316 92-93 -0.5942859972513013 2316 94-95 -0.8971126858142462 2316 96-97 -0.7663200710957092 2316 98-99 -1.076736288423593 2316 100-101 -1.4518506226326764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 17.0 5 49.0 6 119.0 7 330.0 8 838.0 9 1610.0 10 2671.0 11 4458.0 12 6568.0 13 9329.0 14 11720.0 15 16202.0 16 20284.0 17 26357.0 18 22723.0 19 15614.0 20 14230.0 21 14538.0 22 15639.0 23 17899.0 24 21535.0 25 26428.0 26 33418.0 27 42838.0 28 54736.0 29 69254.0 30 86634.0 31 108057.0 32 135541.0 33 171039.0 34 223998.0 35 298300.0 36 423502.0 37 604081.0 38 639084.0 39 164088.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.46084145354364 22.455355371935863 15.718470171456325 20.365333003064176 2 14.499826250782476 22.606982902350516 40.91306727303327 21.980123573833733 3 19.63086145751854 27.80076729990561 30.657231827350557 21.911139415225293 4 12.380841397304309 18.226521661485748 40.494916444447135 28.89772049676281 5 11.940922395188556 39.77916271918009 35.496989820781586 12.782925064849762 6 27.810646230849745 40.445440352182466 18.17487564101555 13.56903777595224 7 25.787565190281462 34.36919416791019 23.127922779595554 16.715317862212792 8 24.43354450494735 36.164041903389744 20.368788573123595 19.03362501853931 9 24.453945317347653 19.25649027329219 22.81570559872147 33.47385881063869 10-11 23.71331794791933 28.03553567528816 28.64025236558686 19.610894011205655 12-13 25.283886082347852 25.69067646551385 28.69088228973391 20.334555162404392 14-15 21.560173495883944 27.772553851965494 27.875544142122667 22.791728510027895 16-17 19.881484597992003 30.976772131400605 28.64576743679229 20.495975833815102 18-19 19.74854636644581 30.80828381949216 30.46681235792832 18.976357456133712 20-21 21.298507126081105 28.606325586098052 29.943253808545666 20.151913479275176 22-23 20.510610541226896 28.825273398127603 30.0597949627549 20.604321097890605 24-25 21.16849133873521 29.092965957279365 30.074505340835827 19.664037363149596 26-27 20.899626976701104 28.37566712385203 30.94841695910486 19.776288940342003 28-29 20.171583525656704 29.93088467136315 30.503041655668113 19.394490147312034 30-31 20.92837614545177 29.04991167555209 29.756749430253958 20.264962748742185 32-33 20.68325253500315 29.24979768255526 30.05955178229296 20.007398000148626 34-35 21.42497463705217 28.595299072404007 30.3517333454012 19.627992945142626 36-37 21.359628919917853 28.364805398056898 30.498291839413984 19.77727384261127 38-39 20.793857289133786 29.198991047934303 30.686309900852976 19.320841762078935 40-41 20.767052717184256 29.22266245748144 29.964698604669326 20.045586220664976 42-43 20.280147951292303 29.731702075495114 29.3653803661855 20.62276960702708 44-45 21.03606766773917 28.753734347522087 29.4103741460565 20.79982383868224 46-47 21.61802705923411 29.49006428679313 29.081866305626665 19.810042348346098 48-49 21.586965273216517 29.13078615771584 29.229021820394152 20.053226748673495 50-51 20.507977201940804 29.361218479382767 29.738391362647143 20.392412956029286 52-53 20.97111196534889 30.274336443902307 28.417529564530437 20.337022026218374 54-55 20.575460304680384 29.78072153496439 28.692123288707815 20.951694871647412 56-57 21.366932551594818 29.081005736667226 28.795118270993775 20.756943440744184 58-59 22.097493115465852 28.739835134400515 28.58245489563173 20.580216854501902 60-61 21.10997443461696 29.533791606181403 29.531899667942135 19.8243342912595 62-63 20.567757622579286 29.415138326364065 29.32527394483898 20.691830106217672 64-65 20.386323701863788 30.412131608970462 28.7107780754675 20.490766613698252 66-67 20.929925227236087 28.86000107477806 28.684464083112132 21.525609614873716 68-69 20.87593034909298 29.49965993014569 28.525004895976764 21.09940482478457 70-71 21.425528376070005 29.298407868445175 28.59978355086456 20.676280204620255 72-73 21.07285874707533 28.62855992662972 29.458954715644758 20.839626610650193 74-75 20.65889791102006 30.281908093720862 28.82547450435326 20.23371949090582 76-77 20.8337091643234 29.45340048852984 28.305052681919857 21.407837665226907 78-79 20.668583758135924 29.61568828764666 28.468551038133633 21.247176916083788 80-81 21.287734153604006 29.582910523149568 28.307435027222542 20.821920296023883 82-83 20.997043092037657 29.219465892040926 29.06205571844933 20.721435297472084 84-85 20.56591109446355 29.48636981040699 28.62151394422311 21.326205150906347 86-87 20.75846684242547 29.265990065894414 27.97183217960088 22.00371091207923 88-89 20.609269959652398 29.256066517545005 28.23697934796098 21.897684174841622 90-91 21.037263264311594 27.414318101332107 28.951204525711383 22.59721410864492 92-93 20.918672191634457 28.379467217952715 28.988555567999175 21.71330502241365 94-95 20.223359066960654 28.64809392019786 29.480109698471463 21.648437314370025 96-97 19.301904919301087 28.846448382177652 29.83335361324454 22.018293085276724 98-99 19.73192427555446 29.803363916594627 28.435849611716073 22.02886219613484 100-101 19.291755372803383 29.257065957411065 28.308460806076013 23.14271786370954 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 158.0 1 120.5 2 133.5 3 185.0 4 176.0 5 184.0 6 269.0 7 489.0 8 836.0 9 1124.0 10 1261.0 11 1328.0 12 1508.0 13 1796.5 14 2126.5 15 2454.0 16 2763.0 17 3135.0 18 3552.0 19 3991.0 20 4425.5 21 4979.5 22 5758.5 23 6826.0 24 8268.5 25 10328.5 26 13523.0 27 18741.0 28 28006.0 29 41257.0 30 56654.5 31 73014.0 32 90277.0 33 105383.0 34 116727.5 35 124799.5 36 129476.0 37 133385.5 38 135992.0 39 131902.0 40 125820.5 41 122394.5 42 121182.5 43 124207.0 44 125419.5 45 125618.5 46 128211.5 47 147300.5 48 186726.0 49 175885.5 50 129467.5 51 103615.0 52 83199.5 53 68773.5 54 57997.0 55 48642.0 56 39294.5 57 30452.0 58 23717.0 59 19040.5 60 15045.0 61 11260.5 62 8189.0 63 5708.0 64 3661.5 65 2299.5 66 1400.5 67 844.5 68 476.5 69 265.0 70 169.5 71 94.5 72 60.5 73 39.5 74 23.0 75 15.5 76 10.5 77 8.5 78 6.5 79 4.5 80 2.0 81 2.0 82 2.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04337442755138795 2 0.0053877516428102274 3 0.04724876581138632 4 0.0 5 0.0 6 0.0014528768474993872 7 0.0 8 0.0 9 0.0 10-11 3.4808507804672814E-4 12-13 0.0 14-15 0.002436595546327097 16-17 0.0 18-19 0.0 20-21 0.012001368125698061 22-23 0.0 24-25 0.0 26-27 0.007355189040465647 28-29 0.009867455255933338 30-31 0.010427418207573726 32-33 0.005887178059138141 34-35 0.007582201047887426 36-37 0.020446214801788248 38-39 0.010972247025385996 40-41 0.011486807575542029 42-43 0.0 44-45 0.0 46-47 3.026826765623723E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0012864013753900823 58-59 2.4214614124989784E-4 60-61 0.009065346163043051 62-63 0.014498500207337634 64-65 0.045629413491777626 66-67 0.023684919441005634 68-69 0.0017252912564055224 70-71 3.4808507804672814E-4 72-73 0.0 74-75 1.5134133828118615E-5 76-77 0.0 78-79 1.5134133828118615E-5 80-81 7.567066914059309E-5 82-83 3.026826765623723E-5 84-85 7.567066914059309E-5 86-87 6.053653531247447E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.9674373976554202E-4 96-97 8.475114943746425E-4 98-99 6.659018884372191E-4 100-101 0.0013469379107025569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3303790.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.46734399403644 #Duplication Level Percentage of deduplicated Percentage of total 1 74.81118759310922 37.75521938863835 2 14.864604009087149 15.003541677234688 3 4.503193788322644 6.817926899612611 4 1.997497705485436 4.03233615320128 5 1.0095957032045881 2.5475806824263536 6 0.5858438715446552 1.773959051522533 7 0.4014227249238509 1.4181117122028815 8 0.28843568673414816 1.1645266418054712 9 0.2016058890015702 0.9157062376319195 >10 1.1538270092971477 10.946304607492026 >50 0.10739543298361422 3.762812248438326 >100 0.0680030745084902 6.281318552474511 >500 0.004624702508047687 1.619104532113683 >1k 0.0019820153598587515 2.1136018981699016 >5k 6.606717866195839E-4 2.419533117992486 >10k+ 1.2012214302174252E-4 1.428416599042831 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35163 1.0643230955962697 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11962 0.36206901770390976 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9380 0.28391635061550524 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8612 0.260670321055515 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8491 0.2570078606691103 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7904 0.23924038755489904 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7627 0.23085607741412137 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7564 0.2289491765517784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6993 0.21166599572006695 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6117 0.18515099325320314 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5893 0.17837090129820599 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5831 0.17649426870351928 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5411 0.16378159628789965 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4378 0.1325144757990066 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3912 0.11840946307120005 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3703 0.11208339513104645 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3306 0.10006689287152028 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.026826765623723E-5 0.0 0.0 0.0 3 0.0 3.026826765623723E-5 0.0 0.0 0.0 4 0.0 3.026826765623723E-5 0.0 3.026826765623723E-5 0.0 5 0.0 3.026826765623723E-5 0.0 6.053653531247446E-5 0.0 6 0.0 3.026826765623723E-5 0.0 6.053653531247446E-5 0.0 7 0.0 3.026826765623723E-5 0.0 6.053653531247446E-5 0.0 8 0.0 3.026826765623723E-5 0.0 6.053653531247446E-5 0.0 9 0.0 3.026826765623723E-5 0.0 1.2107307062494892E-4 0.0 10-11 0.0 3.026826765623723E-5 0.0 1.5134133828118614E-4 0.0 12-13 0.0 3.026826765623723E-5 0.0 1.96743739765542E-4 0.0 14-15 0.0 3.026826765623723E-5 0.0 2.724144089061351E-4 0.0 16-17 0.0 3.026826765623723E-5 0.0 3.026826765623723E-4 0.0 18-19 0.0 3.026826765623723E-5 0.0 4.691581486716771E-4 0.0 20-21 0.0 3.026826765623723E-5 0.0 6.356336207809819E-4 0.0 22-23 0.0 3.026826765623723E-5 0.0 9.080480296871169E-4 0.0 24-25 0.0 6.053653531247446E-5 0.0 0.0013469379107025566 0.0 26-27 0.0 9.08048029687117E-5 0.0 0.002088510468280369 0.0 28-29 0.0 9.08048029687117E-5 0.0 0.0047369838882011265 0.0 30-31 0.0 9.08048029687117E-5 0.0 0.009988528326558286 0.0 32-33 0.0 9.08048029687117E-5 0.0 0.01822149712905481 0.0 34-35 0.0 9.08048029687117E-5 0.0 0.025924771247567188 0.0 36-37 0.0 9.08048029687117E-5 0.0 0.03533820248865696 0.0 38-39 0.0 9.08048029687117E-5 0.0 0.045659681759433864 0.0 40-41 0.0 1.2107307062494892E-4 0.0 0.0666658595128625 0.0 42-43 0.0 1.2107307062494892E-4 0.0 0.09066859576425862 0.0 44-45 0.0 1.2107307062494892E-4 0.0 0.14077771286915935 0.0 46-47 0.0 1.2107307062494892E-4 0.0 0.23506336661833832 0.0 48-49 0.0 1.2107307062494892E-4 0.0 0.297673278265265 0.0 50-51 0.0 1.5134133828118614E-4 0.0 0.3739190444913266 0.0 52-53 0.0 1.5134133828118614E-4 0.0 0.47257846291683187 0.0 54-55 0.0 1.5134133828118614E-4 0.0 0.5803486299068645 0.0 56-57 0.0 1.5134133828118614E-4 0.0 0.6836088250161179 0.0 58-59 0.0 1.5134133828118614E-4 0.0 0.8918242382233738 0.0 60-61 0.0 1.5134133828118614E-4 0.0 1.0847390421304017 0.0 62-63 0.0 1.5134133828118614E-4 0.0 1.3000826323707015 0.0 64-65 0.0 1.5134133828118614E-4 0.0 1.4261499671589295 0.0 66-67 0.0 1.5134133828118614E-4 0.0 1.7975264771671324 0.0 68-69 0.0 1.5134133828118614E-4 0.0 2.19661358621462 0.0 70-71 0.0 1.5134133828118614E-4 0.0 2.353615090547523 0.0 72-73 0.0 2.4214614124989784E-4 0.0 2.7911126312507757 0.0 74-75 0.0 2.4214614124989784E-4 0.0 3.0184575896167734 0.0 76-77 0.0 2.4214614124989784E-4 0.0 3.167528807823742 0.0 78-79 0.0 3.026826765623723E-4 0.0 3.347942817188744 0.0 80-81 0.0 3.026826765623723E-4 0.0 3.534894772367493 0.0 82-83 0.0 3.026826765623723E-4 0.0 3.870675799611961 0.0 84-85 0.0 3.026826765623723E-4 0.0 4.530584571053245 0.0 86-87 0.0 3.026826765623723E-4 0.0 5.707959646345561 0.0 88-89 0.0 3.026826765623723E-4 0.0 7.0018826862482175 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2775 0.0 34.415573 2 ACCTGGG 3020 0.0 30.67727 3 GTACCTG 4030 0.0 24.180288 1 TAGACCG 120 1.71378E-4 23.745874 5 ACGCGAT 145 2.3394721E-5 22.926702 8 TCACGCA 375 0.0 22.796038 4 TCGATCG 105 0.0021989208 22.6158 9 TAACGCA 495 0.0 22.06687 4 GTACACG 390 0.0 21.93919 1 ATAACGC 585 0.0 20.303608 3 ACGTGCG 260 1.4950274E-8 20.093267 9 TATAACG 605 0.0 19.6339 2 GAGTACT 10335 0.0 19.323544 12-13 GTACCCG 320 3.929017E-10 19.311056 1 AGTACTT 10630 0.0 19.278748 12-13 GTATAAC 1060 0.0 18.834587 1 TACACGG 435 0.0 18.568705 2 CCTGGGG 2305 0.0 18.543415 4 GTACTTT 10880 0.0 18.074726 14-15 CTAACGC 185 1.51987E-4 17.976925 3 >>END_MODULE