FastQCFastQC Report
Wed 25 May 2016
SRR1003806_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003806_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092538
Sequences flagged as poor quality0
Sequence length121
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT84610.40434152211333796No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT79840.3815462371531604No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55170.26365112604884594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48140.2300555593255654No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48010.2294343041799002No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35320.16879024419150332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34020.16257769273485118No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32060.15321107669251408No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27260.13027242516025994No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23360.11163477079030346No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC22530.10766829562951785No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21290.10174247731701885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG23600.056.0665972
ACCTGGG25750.050.6477743
GTACCTG37950.035.6853521
GTACACG2600.031.0304851
TATAACG2650.030.392792
CCTGGGG23100.029.8434874
CTGGGGG16250.025.1024865
GTACCGG1701.7924885E-523.7291971
GCGTCGG2702.590241E-923.3909249
GTACCCG2252.6048838E-723.0512181
TAACGCA3503.6379788E-1222.9794864
ACCGTGA3555.456968E-1222.6449058
GTACATG78850.022.071871
TACACGG3207.6579454E-1021.573452
GTACTAG5700.021.2313841
GAGTACT58500.021.06681412-13
GTATAAC8350.020.7046891
TACATGG76650.020.4898532
GGACCGT1704.9827574E-420.2779736
AGTACTT61950.019.98634712-13
Produced by FastQC (version 0.11.5)