##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003806_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2092538 Sequences flagged as poor quality 0 Sequence length 121 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.74944779975322 31.0 31.0 34.0 27.0 34.0 2 30.678977394914693 31.0 31.0 34.0 27.0 34.0 3 30.733427541100806 31.0 31.0 34.0 27.0 34.0 4 34.223603585693546 37.0 35.0 37.0 30.0 37.0 5 34.17933246612487 37.0 35.0 37.0 30.0 37.0 6 34.27821430244038 37.0 35.0 37.0 30.0 37.0 7 34.3179865789773 37.0 35.0 37.0 30.0 37.0 8 34.271245253371745 37.0 35.0 37.0 30.0 37.0 9 35.7329138108842 39.0 35.0 39.0 30.0 39.0 10-11 35.57626121962899 39.0 35.0 39.0 30.0 39.0 12-13 35.42779247019648 38.0 35.0 39.0 30.0 39.0 14-15 36.42296914082325 39.0 36.0 41.0 29.0 41.0 16-17 36.48768313884861 39.0 36.0 41.0 30.0 41.0 18-19 36.46269745161139 39.0 36.0 41.0 29.5 41.0 20-21 36.34925148312719 39.0 36.0 41.0 29.0 41.0 22-23 36.263272877242855 39.0 36.0 41.0 29.0 41.0 24-25 36.08703187230053 39.0 36.0 41.0 27.0 41.0 26-27 35.904825145349804 39.0 35.0 41.0 27.0 41.0 28-29 35.71050919983293 39.0 35.0 40.0 27.0 41.0 30-31 35.51152332717494 38.5 35.0 40.0 26.5 41.0 32-33 35.28970895630091 38.0 34.0 40.0 26.0 41.0 34-35 35.282963559084706 38.0 35.0 40.0 25.5 41.0 36-37 35.17550768492615 38.0 34.0 40.0 25.0 41.0 38-39 35.209265016931596 38.0 34.5 40.0 25.0 41.0 40-41 35.278442733178565 39.0 35.0 40.0 25.0 41.0 42-43 35.14384278804017 39.0 35.0 40.0 25.0 41.0 44-45 34.95454443360168 38.0 34.0 40.0 24.0 41.0 46-47 34.70054426729646 38.0 34.0 40.0 23.5 41.0 48-49 34.46385728717949 38.0 34.0 40.0 23.0 41.0 50-51 33.74686290045868 37.5 33.0 39.5 22.0 40.5 52-53 33.89746183820796 38.0 33.0 40.0 22.5 40.5 54-55 34.239002350256 38.0 34.0 40.0 22.5 41.0 56-57 34.1013429624695 38.0 33.5 40.0 21.5 41.0 58-59 33.853901100003924 38.0 33.0 40.0 20.0 41.0 60-61 33.619100107142614 38.0 33.0 40.0 19.5 41.0 62-63 33.371435548601745 37.5 33.0 40.0 18.5 41.0 64-65 33.08697954350172 37.0 32.0 40.0 18.0 41.0 66-67 32.76295436450856 36.5 32.0 39.5 16.5 41.0 68-69 32.423739736148164 36.0 31.5 39.0 13.0 41.0 70-71 32.059004663236706 35.5 31.0 39.0 12.5 40.5 72-73 31.71591220804592 35.0 31.0 38.5 9.0 40.0 74-75 31.34637100974988 35.0 31.0 37.5 8.0 40.0 76-77 30.921370842488884 35.0 30.5 37.0 7.0 39.0 78-79 30.39169300629188 35.0 30.0 36.5 6.0 39.0 80-81 30.02283518865607 34.0 29.5 36.0 2.0 39.0 82-83 29.691000832481897 34.0 29.0 36.0 2.0 37.5 84-85 29.447078380416507 34.0 29.0 35.0 2.0 37.0 86-87 29.19215588916426 34.0 29.0 35.0 2.0 37.0 88-89 28.875929612747775 34.0 29.0 35.0 2.0 36.0 90-91 28.550523335776937 34.0 29.0 35.0 2.0 36.0 92-93 28.303703445289884 34.0 28.5 35.0 2.0 36.0 94-95 28.080550986409804 34.0 27.5 35.0 2.0 35.0 96-97 27.802817678818734 34.0 27.0 35.0 2.0 35.0 98-99 27.53991062527897 34.0 26.0 35.0 2.0 35.0 100-101 27.26962951210444 34.0 25.5 35.0 2.0 35.0 102-103 27.0176073266053 33.0 25.0 35.0 2.0 35.0 104-105 26.73611685904868 33.0 25.0 35.0 2.0 35.0 106-107 26.42077467649333 33.0 25.0 35.0 2.0 35.0 108-109 26.089309250297966 33.0 24.0 35.0 2.0 35.0 110-111 25.70491718668908 32.0 21.5 35.0 2.0 35.0 112-113 25.269625450051564 32.0 19.0 35.0 2.0 35.0 114-115 24.791762204557337 32.0 17.5 34.0 2.0 35.0 116-117 24.181339837078227 32.0 4.5 34.0 2.0 35.0 118-119 23.45453822105023 31.0 2.0 34.0 2.0 35.0 120-121 21.79363218254579 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5477855269447431 1101 2 0.5520887416955276 1101 3 0.5338059578902019 1101 4 0.8090638066317482 1101 5 0.7338454655509139 1101 6 0.8223731032159378 1101 7 0.69301132704868 1101 8 0.7232304475672464 1101 9 0.8948343707064694 1101 10-11 0.8302193415520023 1101 12-13 0.9122156650430711 1101 14-15 1.0433730511463963 1101 16-17 0.8275936188162305 1101 18-19 0.8993460536274682 1101 20-21 0.9039770418748958 1101 22-23 0.7863273555529204 1101 24-25 0.677033856214976 1101 26-27 0.7956685865521465 1101 28-29 0.9432557677974742 1101 30-31 0.8832484737435919 1101 32-33 0.7980751854006911 1101 34-35 0.8081264994864554 1101 36-37 0.7222621062917014 1101 38-39 0.7397923376783169 1101 40-41 0.792174431720845 1101 42-43 0.8088533934175501 1101 44-45 0.8387295306309355 1101 46-47 0.82097890117916 1101 48-49 0.9025825969835637 1101 50-51 0.7307048778175016 1101 52-53 0.7280804647350081 1101 54-55 0.6538219830030201 1101 56-57 0.8059269585577624 1101 58-59 0.7920048873812178 1101 60-61 0.7133694594552793 1101 62-63 0.6718660061430697 1101 64-65 0.6147324851960221 1101 66-67 0.6721234873197304 1101 68-69 0.5787876815603141 1101 70-71 0.4341282603454779 1101 72-73 0.4693129285892006 1101 74-75 0.4003949388304342 1101 76-77 0.4040627444886091 1101 78-79 0.6140802751197505 1101 80-81 0.5837959225152467 1101 82-83 0.6452092864228831 1101 84-85 0.6914150322918751 1101 86-87 0.595073606305327 1101 88-89 0.6872990632310625 1101 90-91 0.7487015489250801 1101 92-93 0.709823917279099 1101 94-95 0.773973268691666 1101 96-97 0.7584231165706932 1101 98-99 0.6796902190324694 1101 100-101 0.6317587848502022 1101 102-103 0.4792910577810936 1101 104-105 0.555388156589725 1101 106-107 0.5595728825352531 1101 108-109 0.4114139368995815 1101 110-111 0.3742533715182432 1101 112-113 0.5571659309713937 1101 114-115 0.5300692692934525 1101 116-117 0.47520640168720263 1101 118-119 0.736593163092504 1101 120-121 0.6878018442749578 1102 1 0.5011736674314413 1102 2 0.4432703803647975 1102 3 0.5673970940044661 1102 4 0.7770614655695098 1102 5 0.882544625882268 1102 6 0.7647470727112093 1102 7 0.5881167440816313 1102 8 0.734046209143628 1102 9 0.6943008773397636 1102 10-11 0.7235552843209305 1102 12-13 0.8249226989354526 1102 14-15 0.8840657414733712 1102 16-17 0.9699982193479926 1102 18-19 0.9230010995250453 1102 20-21 1.0053269351053018 1102 22-23 0.7092082332418741 1102 24-25 0.9343591318183115 1102 26-27 0.8962703496435367 1102 28-29 0.834084997573818 1102 30-31 0.8167068528518016 1102 32-33 0.6846837825566254 1102 34-35 0.7503972960859144 1102 36-37 0.7769341161098495 1102 38-39 0.7567256103124365 1102 40-41 0.8162582108139915 1102 42-43 0.7678105062515499 1102 44-45 0.848912746106258 1102 46-47 0.8820570866465403 1102 48-49 0.8940687223398172 1102 50-51 0.3676713717863649 1102 52-53 0.6733523937034249 1102 54-55 0.6415142670550509 1102 56-57 0.8127899824388223 1102 58-59 0.7577474333505734 1102 60-61 0.9450113539325358 1102 62-63 0.7860160928184357 1102 64-65 0.7338116527477965 1102 66-67 0.6914507595127901 1102 68-69 0.5240123299872721 1102 70-71 0.45303760632387835 1102 72-73 0.41441515418560115 1102 74-75 0.3867263031543615 1102 76-77 0.36364877295352116 1102 78-79 0.65161798557304 1102 80-81 0.7735258121590824 1102 82-83 0.8614536815679834 1102 84-85 0.5749353657953051 1102 86-87 0.7509612663879537 1102 88-89 0.7536976604849421 1102 90-91 0.8271647050565711 1102 92-93 0.9010849366002951 1102 94-95 0.5898896888522245 1102 96-97 0.6760874308052749 1102 98-99 0.7989997947943017 1102 100-101 0.7670940157092687 1102 102-103 0.78585572455966 1102 104-105 0.7185397120013342 1102 106-107 0.7635888410688665 1102 108-109 0.6635928099948956 1102 110-111 0.7259386188748422 1102 112-113 0.5962741152668904 1102 114-115 0.4529937970537148 1102 116-117 0.5782684415251111 1102 118-119 0.5661960335617238 1102 120-121 0.6432098770363233 1103 1 0.5571777742778465 1103 2 0.5139800306360485 1103 3 0.6217250811516131 1103 4 0.7998729008022991 1103 5 0.9360945871854227 1103 6 0.9407099029261232 1103 7 0.8420802895004229 1103 8 0.6540065681135303 1103 9 0.7134701256545313 1103 10-11 0.7881150733580924 1103 12-13 0.8024664494140481 1103 14-15 1.0954214989017856 1103 16-17 0.9940462064059332 1103 18-19 0.8161383391205774 1103 20-21 1.0014925650237245 1103 22-23 0.9222940108699689 1103 24-25 1.0492097605211939 1103 26-27 1.1916313380225816 1103 28-29 1.1437184667364093 1103 30-31 0.9039676740277898 1103 32-33 0.9038406151699263 1103 34-35 0.8265287791079956 1103 36-37 0.9345670589061754 1103 38-39 0.8068569788424256 1103 40-41 0.9132348759863191 1103 42-43 0.8196510782362196 1103 44-45 0.8263230463803737 1103 46-47 0.7401974747747531 1103 48-49 0.7684322317753782 1103 50-51 0.700159210718283 1103 52-53 0.7036245875132678 1103 54-55 0.7535851168466294 1103 56-57 0.7118709603202902 1103 58-59 0.7622326984406698 1103 60-61 0.7732973700610515 1103 62-63 0.8234851186170786 1103 64-65 0.693808745838048 1103 66-67 0.6920353011466531 1103 68-69 0.5392626401939395 1103 70-71 0.5209823467873953 1103 72-73 0.401268636269446 1103 74-75 0.6611147926839145 1103 76-77 0.6706117511160166 1103 78-79 0.8317437930582976 1103 80-81 0.8535184296491636 1103 82-83 0.8911286950059356 1103 84-85 0.9608610901622718 1103 86-87 1.0444413131661534 1103 88-89 0.9283505226758599 1103 90-91 0.8439337083285174 1103 92-93 1.0380981500291746 1103 94-95 1.1884185025728407 1103 96-97 1.1693645514925848 1103 98-99 1.0706637069595324 1103 100-101 1.1101643815115168 1103 102-103 1.0607226274963857 1103 104-105 1.0314947530363447 1103 106-107 1.0766918296843393 1103 108-109 0.9205205112091797 1103 110-111 1.1599408016592463 1103 112-113 1.0490541172147445 1103 114-115 1.0926183967960341 1103 116-117 1.1389230463749662 1103 118-119 1.2156918594326243 1103 120-121 1.3950380333195191 1104 1 0.32512539718881683 1104 2 0.5105924596865989 1104 3 0.4905955921145271 1104 4 0.758543187011 1104 5 0.7386348490391512 1104 6 0.671952165599869 1104 7 0.527917284804694 1104 8 0.567731563811158 1104 9 0.6161235576766941 1104 10-11 0.6814674560778684 1104 12-13 0.6942247744003893 1104 14-15 0.7946795086453662 1104 16-17 0.6720574488748028 1104 18-19 0.6496992816924703 1104 20-21 0.7234706558501216 1104 22-23 0.6456298264922182 1104 24-25 0.7160132306500557 1104 26-27 0.7462151263761285 1104 28-29 0.5630307934024543 1104 30-31 0.7435102984178172 1104 32-33 0.7866541746532647 1104 34-35 0.5836891516258049 1104 36-37 0.6178291751328118 1104 38-39 0.3062699135502953 1104 40-41 0.4908950292220524 1104 42-43 0.5102196889395714 1104 44-45 0.5519226619031627 1104 46-47 0.6100771591037386 1104 48-49 0.8181424463545852 1104 50-51 0.44375561909208017 1104 52-53 0.6095275974273591 1104 54-55 0.6008571368508626 1104 56-57 0.6030902003592118 1104 58-59 0.6350042890009888 1104 60-61 0.5201344343618501 1104 62-63 0.6598657986373482 1104 64-65 0.5695868403125317 1104 66-67 0.4360048459202446 1104 68-69 0.5210745207617435 1104 70-71 0.4251086960858892 1104 72-73 0.31086276220635156 1104 74-75 0.28639107619778414 1104 76-77 0.31455932802930064 1104 78-79 0.3585303074884827 1104 80-81 0.3962552364422294 1104 82-83 0.27864888838655233 1104 84-85 0.5218718655167116 1104 86-87 0.5168750190014393 1104 88-89 0.5193392984474521 1104 90-91 0.524755766280844 1104 92-93 0.6454985828832598 1104 94-95 0.6127759030056943 1104 96-97 0.44586338212038257 1104 98-99 0.5212869756026741 1104 100-101 0.5323791225009273 1104 102-103 0.4819636847135378 1104 104-105 0.4322244111545821 1104 106-107 0.3139350059672985 1104 108-109 0.3588800433968551 1104 110-111 0.4456248943112726 1104 112-113 0.34588214059108324 1104 114-115 0.4654165549852465 1104 116-117 0.27864200844511444 1104 118-119 0.3043714324154898 1104 120-121 0.43118606814769933 1105 1 0.780072812353005 1105 2 0.9461240400410311 1105 3 0.9196787898887884 1105 4 1.1910243484539222 1105 5 1.2553133724043946 1105 6 1.1215989568147364 1105 7 1.0728497922472258 1105 8 1.0743793201763907 1105 9 1.2887413807868384 1105 10-11 1.267416815136464 1105 12-13 1.3886946440371233 1105 14-15 1.5797629511056357 1105 16-17 1.5393209530503214 1105 18-19 1.3723662685655214 1105 20-21 1.4905329737516197 1105 22-23 1.4341560917227838 1105 24-25 1.447195290634376 1105 26-27 1.4980113029306779 1105 28-29 1.6168687387649783 1105 30-31 1.4930018952659054 1105 32-33 1.5140062189142398 1105 34-35 1.5089085760699348 1105 36-37 1.4726952548551395 1105 38-39 1.4539154903975628 1105 40-41 1.5902006193911191 1105 42-43 1.618569372433683 1105 44-45 1.6839316699732692 1105 46-47 1.7564542202435192 1105 48-49 1.778402266646303 1105 50-51 1.4169781338298861 1105 52-53 1.4179747022028124 1105 54-55 1.5329893941699169 1105 56-57 1.6335121477879753 1105 58-59 1.5741610738268363 1105 60-61 1.6482581457954808 1105 62-63 1.7101526250144943 1105 64-65 1.7378784788478612 1105 66-67 1.7046460682627966 1105 68-69 1.6110419412727737 1105 70-71 1.3653294119284034 1105 72-73 1.28725441388222 1105 74-75 1.2736868879889336 1105 76-77 1.4665585033468282 1105 78-79 1.545860330008658 1105 80-81 1.603576105893179 1105 82-83 1.8029702245534125 1105 84-85 1.6291616960943074 1105 86-87 1.6517067520580646 1105 88-89 1.611991241662171 1105 90-91 1.5784095174532986 1105 92-93 1.747958356956513 1105 94-95 1.9009258502169537 1105 96-97 1.872863576962942 1105 98-99 1.9631529782942216 1105 100-101 1.8227822200445516 1105 102-103 2.018823819472093 1105 104-105 2.0552781378584335 1105 106-107 1.810183606952382 1105 108-109 1.7962198080451621 1105 110-111 1.8088984306131621 1105 112-113 1.69820643111494 1105 114-115 1.6989539443667816 1105 116-117 1.6765814350642358 1105 118-119 1.73392882261264 1105 120-121 1.60623761080236 1106 1 0.43311233497886903 1106 2 0.5647065091875803 1106 3 0.48505508552898746 1106 4 0.8358495954223386 1106 5 0.888078317865272 1106 6 0.7640892995114115 1106 7 0.8177551647541819 1106 8 0.8506596008461713 1106 9 0.7939549166270155 1106 10-11 0.9321701539138942 1106 12-13 1.0408416500810986 1106 14-15 0.9583479289751153 1106 16-17 0.8977131432422354 1106 18-19 0.9017914126250943 1106 20-21 1.051734933627415 1106 22-23 0.8599950419502349 1106 24-25 1.0313498894051065 1106 26-27 1.0768906717564661 1106 28-29 1.0836637428224591 1106 30-31 1.0017482505778688 1106 32-33 1.0772680195117275 1106 34-35 1.0581231921969305 1106 36-37 1.024156073978773 1106 38-39 1.1205763545758174 1106 40-41 0.9761152920923664 1106 42-43 0.9118964254266189 1106 44-45 0.7609362803916397 1106 46-47 0.8605241112164634 1106 48-49 1.0537508639373954 1106 50-51 0.7671468326474624 1106 52-53 0.5939353766908937 1106 54-55 0.8218210972599991 1106 56-57 0.800226514322631 1106 58-59 0.9367569108827212 1106 60-61 0.9167522976551368 1106 62-63 0.8998416457677791 1106 64-65 0.6901061224146545 1106 66-67 0.8420808222671567 1106 68-69 0.6693102312709129 1106 70-71 0.6682973847522753 1106 72-73 0.6432698450881809 1106 74-75 0.5319878169843939 1106 76-77 0.7161074452033311 1106 78-79 0.87461989451484 1106 80-81 0.698128209252566 1106 82-83 0.6926228944233941 1106 84-85 0.9131035654000819 1106 86-87 0.8831932727921483 1106 88-89 0.8854986155156475 1106 90-91 0.7488323140649875 1106 92-93 0.6716774363063927 1106 94-95 0.8158053931173939 1106 96-97 0.8813882430852011 1106 98-99 0.9799909890305294 1106 100-101 0.8577617534732838 1106 102-103 0.7087171219498423 1106 104-105 0.8785675344081731 1106 106-107 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-0.9153247934166089 2316 68-69 -1.3072059320002225 2316 70-71 -1.146403700809735 2316 72-73 -1.259066873142583 2316 74-75 -1.1866282009693698 2316 76-77 -1.2741987225862808 2316 78-79 -1.2131527945823528 2316 80-81 -1.3162484468459432 2316 82-83 -1.223146229955006 2316 84-85 -1.2100503345750084 2316 86-87 -0.9049746895479984 2316 88-89 -0.8959489507001273 2316 90-91 -0.9444733242737655 2316 92-93 -1.1192493029647146 2316 94-95 -1.1158706630619726 2316 96-97 -0.9006285766799635 2316 98-99 -0.6045399087454015 2316 100-101 -0.6411529348453762 2316 102-103 -0.5552634473287412 2316 104-105 -0.6734008837854155 2316 106-107 -0.8860092739138636 2316 108-109 -0.9081626694528921 2316 110-111 -0.9800845679175758 2316 112-113 -0.8388220775216126 2316 114-115 -0.7622951759275622 2316 116-117 -0.947231733975066 2316 118-119 -0.8577618173192043 2316 120-121 -1.1928116897544747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29656.0 3 4593.0 4 4085.0 5 4718.0 6 5498.0 7 5646.0 8 7014.0 9 8179.0 10 10260.0 11 12125.0 12 12871.0 13 14889.0 14 18525.0 15 18392.0 16 13727.0 17 13091.0 18 13896.0 19 14914.0 20 16261.0 21 18261.0 22 20742.0 23 23277.0 24 26636.0 25 30175.0 26 34856.0 27 40775.0 28 48028.0 29 57104.0 30 67934.0 31 81739.0 32 100214.0 33 125940.0 34 162081.0 35 216334.0 36 300699.0 37 360957.0 38 147817.0 39 629.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84598227139583 20.31936825051923 14.828126979495782 22.00652249858916 2 14.073485794247684 20.62422275938921 41.902352299571376 23.39993914679173 3 18.243907943543956 26.954299690791846 30.916716336722473 23.88507602894173 4 11.528277708084842 17.023418433712052 41.08258789747071 30.3657159607324 5 11.399132864920954 37.82910534263986 36.75998912223788 14.011772670201298 6 27.611167315888096 38.25407495349042 19.53687156603011 14.597886164591376 7 25.429639574711178 32.963451289678 24.34347062098821 17.263438514622617 8 23.762362397007138 35.08373193633085 21.694913130014072 19.458992536647937 9 23.857941424143057 18.227145452468392 23.702201234063864 34.21271188932469 10-11 23.13155278679833 26.76663915314588 29.582296555588357 20.519511504467435 12-13 24.34112588624494 24.567183264813206 29.648822995385586 21.442867853556272 14-15 21.278406318044723 26.31300783128102 28.417054575557582 23.991531275116674 16-17 19.86421352682042 29.754304344419868 29.098826663131188 21.282655465628523 18-19 19.67452542996136 29.27433735012388 31.000717355790385 20.050419864124375 20-21 20.510776047056417 27.834818393203477 30.409988397919534 21.24441716182057 22-23 20.212391564888478 27.607504669085497 30.62999630715592 21.550107458870105 24-25 20.655912747431532 27.79577596336356 30.44755506010666 21.10075622909825 26-27 20.346637652850397 27.438423574079234 31.12450070236703 21.09043807070334 28-29 19.977574550996803 28.26773411353902 30.935740553321793 20.81895078214238 30-31 20.312873331483278 27.91327847939819 30.432312884904384 21.34153530421415 32-33 20.2196051013766 27.913522505364156 30.72169049398491 21.14518189927433 34-35 20.884231429604082 27.428997442024816 30.67766691638008 21.009104211991016 36-37 20.88588120796371 27.386971631622963 30.912256824913936 20.814890335499392 38-39 20.731244808726043 27.944237429733203 30.74524090524968 20.57927685629107 40-41 20.285008481534128 28.169230416818873 30.702333717925995 20.843427383721 42-43 20.11848044132397 28.373847504050616 29.912574261245272 21.59509779338014 44-45 20.24127846149544 28.099269183334798 29.836055614965012 21.82339674020475 46-47 20.778207115325745 28.223675488307848 29.729878920383463 21.268238475982944 48-49 20.931804512456324 28.020090181628838 29.67643332983792 21.371671976076918 50-51 20.237621575427525 28.408223362641166 30.083766295459817 21.27038876647149 52-53 20.371916336542846 28.801807824181676 29.30344817777195 21.522827661503534 54-55 20.156857506993227 28.603698633373725 29.561484680486434 21.677959179146615 56-57 20.886342825108 28.06782030759255 29.28666471755771 21.759172149741744 58-59 21.2419140693385 27.657343180087473 29.470959614836488 21.629783135737533 60-61 20.82389636624412 27.963030804527623 30.21765297895437 20.99541985027389 62-63 20.330747320561912 28.311125834137396 29.87910402348483 21.47902282181586 64-65 20.10146980795901 28.79452894235106 29.76713677089669 21.336864478793238 66-67 20.252118161403356 28.055558569016082 29.469811755147894 22.222511514432668 68-69 20.142821353470552 28.37824510962489 29.514435850590232 21.964497686314328 70-71 20.68075432644929 27.951161536285845 29.54999043938558 21.818093697879277 72-73 20.260701420264994 27.60282447035152 30.357317249905535 21.779156859477947 74-75 20.091857697253985 28.578638865316776 29.88739160745906 21.44211182997018 76-77 20.273562726047526 28.09390263785221 29.70711862183003 21.925416014270237 78-79 20.182789715086088 28.230687068660533 29.77823618929392 21.808287026959455 80-81 20.453244646811335 28.253306975856063 29.374409026752 21.9190393505806 82-83 20.16491153560294 27.961047673285005 30.062668314012413 21.811372477099642 84-85 20.359228572524366 28.020074693955234 29.577706341267383 22.042990392253017 86-87 20.606445299075833 28.17952339736316 29.325799693577103 21.888231609983897 88-89 20.389643588377012 27.472465459736988 29.254933548127266 22.882957403758734 90-91 20.204081041982125 26.580027775513265 30.06477415632723 23.151117026177385 92-93 20.313921330116596 26.921915650342783 30.34099382536818 22.423169194172445 94-95 19.9325385017162 27.025954928890044 30.440385458449303 22.601121110944455 96-97 19.38945337021923 27.462346987722842 30.528874966507747 22.619324675550185 98-99 19.411508550653135 28.02544267135987 29.73400514595278 22.829043632034217 100-101 19.087233518535896 27.67923974400508 30.299132732898475 22.934394004560552 102-103 19.580832243910994 27.39067010748083 30.272291679176632 22.756205969431544 104-105 20.07338062049449 27.541990343233802 29.60610965040037 22.778519385871338 106-107 19.973345017092804 27.19722155441424 30.26013324597842 22.569300182514535 108-109 20.202149535775117 27.16284619138835 29.991888362259377 22.64311591057715 110-111 20.270270596112944 27.028771588698152 29.240574057369567 23.460383757819336 112-113 20.688147554377444 26.217674731766227 28.75814250947005 24.33603520438627 114-115 20.31354302118744 26.22854632026948 29.454074269861053 24.003836388682025 116-117 20.788416246454137 26.06508588112434 29.19603611105817 23.950461761363346 118-119 20.865872688313765 26.255053223630426 29.42348082470636 23.455593263349446 120-121 20.7670794294425 27.475039254948197 28.714268109547774 23.04361320606153 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5382.0 1 5671.5 2 3623.5 3 1298.5 4 1203.0 5 1131.0 6 1180.5 7 1338.0 8 1606.5 9 1701.0 10 1585.0 11 1527.5 12 1604.0 13 1709.5 14 1851.0 15 1998.5 16 2175.0 17 2413.0 18 2648.0 19 2895.0 20 3241.0 21 3581.0 22 4110.5 23 4928.5 24 5991.5 25 7427.0 26 9523.5 27 12854.0 28 18013.0 29 25213.0 30 34116.5 31 42976.5 32 50992.5 33 57674.0 34 62574.0 35 67115.5 36 70834.5 37 74460.5 38 77183.0 39 77127.5 40 77475.5 41 77833.5 42 78456.5 43 81620.0 44 82848.5 45 83325.0 46 85645.5 47 91968.5 48 107639.0 49 104241.0 50 83870.0 51 70697.0 52 58607.0 53 49647.0 54 42557.5 55 35896.0 56 29586.5 57 23311.0 58 18277.0 59 14862.5 60 12085.0 61 9345.0 62 6909.0 63 4858.5 64 3263.5 65 2214.5 66 1419.5 67 825.5 68 495.5 69 317.0 70 180.5 71 110.0 72 78.0 73 55.0 74 43.0 75 33.5 76 27.0 77 17.0 78 16.5 79 16.0 80 11.0 81 9.5 82 10.0 83 8.0 84 5.0 85 4.5 86 4.5 87 3.0 88 2.5 89 3.0 90 2.0 91 2.0 92 1.5 93 2.0 94 2.0 95 1.0 96 1.5 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41452054873077576 2 0.8934126883239397 3 0.9010111166439988 4 0.8774034211087207 5 0.8880125474423881 6 0.8970924303405721 7 0.8689447933562019 8 0.8602472213168889 9 0.8568064235870507 10-11 0.8506655554164368 12-13 0.8527204762828681 14-15 0.8528160539975858 16-17 0.8757547055298399 18-19 0.9056227413791291 20-21 0.8970924303405721 22-23 0.8724333799433989 24-25 0.8650022126240957 26-27 0.8661252507720291 28-29 0.8867222482936988 30-31 0.8949658261881026 32-33 0.8882992805865414 34-35 0.899744711923989 36-37 0.9095175332538764 38-39 0.8958260256205621 40-41 0.9039501313715689 42-43 0.8973791634847252 44-45 0.8802707525502523 46-47 0.8737236790920881 48-49 0.8788609812581659 50-51 0.9094697443965176 52-53 0.8813460018408268 54-55 0.8958021311918828 56-57 0.9460282202760475 58-59 0.9093741666817997 60-61 0.9248338620373919 62-63 0.8931259551797864 64-65 0.8804380135510085 66-67 0.8845956441412294 68-69 0.9031377207964683 70-71 0.9044758088025163 72-73 0.908776805964814 74-75 0.9134123251286238 76-77 0.9276772990502443 78-79 0.9867921156031575 80-81 1.0165884681664084 82-83 0.9805317752891465 84-85 0.94046081839374 86-87 0.9203894983030178 88-89 0.9191230935830078 90-91 0.9313570410668767 92-93 0.9183106830079071 94-95 0.9108078324025657 96-97 0.9222054748826545 98-99 0.9574736516134951 100-101 0.9444272935545256 102-103 0.9171398560026149 104-105 0.9063634686681914 106-107 0.9082033396765077 108-109 0.9067457795270623 110-111 0.9038067647994924 112-113 0.9156345069958108 114-115 0.9191708824403667 116-117 0.949875223293436 118-119 0.9427546835469653 120-121 0.9190991991543284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 2092538.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.60081587333672 #Duplication Level Percentage of deduplicated Percentage of total 1 79.73903647545659 46.727725944155125 2 12.208570159551517 14.308643439931831 3 3.4395707098411297 6.046849495521663 4 1.474397878405725 3.4560367438596877 5 0.7698523475598327 2.2556987834504887 6 0.49202529305337256 1.7299850161947135 7 0.33156416841188063 1.3600951548310434 8 0.23722575297512014 1.1121298136406939 9 0.17207253001442613 0.9075231583411132 >10 1.0243081948232802 11.106415587294984 >50 0.07332016887739871 2.9819194170143914 >100 0.03375621532761636 3.4659291689052623 >500 0.001901969829713409 0.8161074191166388 >1k 0.002150052850980374 2.660672041244524 >5k 2.4808302126696623E-4 1.0642688164978096 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8461 0.40434152211333796 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7984 0.3815462371531604 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5517 0.26365112604884594 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4814 0.2300555593255654 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4801 0.2294343041799002 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3532 0.16879024419150332 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3402 0.16257769273485118 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3206 0.15321107669251408 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2726 0.13027242516025994 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2336 0.11163477079030346 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2253 0.10766829562951785 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2129 0.10174247731701885 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 4.7788857358862776E-5 0.0 0.0 0.0 16-17 0.0 9.557771471772555E-5 0.0 0.0 0.0 18-19 0.0 9.557771471772555E-5 0.0 7.168328603829416E-5 0.0 20-21 0.0 9.557771471772555E-5 0.0 2.3894428679431388E-4 0.0 22-23 0.0 9.557771471772555E-5 0.0 6.451495743446475E-4 0.0 24-25 0.0 9.557771471772555E-5 0.0 0.0016965044362396286 0.0 26-27 0.0 9.557771471772555E-5 0.0 0.0030584868709672176 0.0 28-29 0.0 9.557771471772555E-5 0.0 0.00721611746118828 0.0 30-31 0.0 9.557771471772555E-5 0.0 0.017634088365420367 0.0 32-33 0.0 9.557771471772555E-5 0.0 0.03937801846370293 0.0 34-35 0.0 9.557771471772555E-5 0.0 0.0626511919974691 0.0 36-37 0.0 9.557771471772555E-5 0.0 0.08109769093799013 0.0 38-39 0.0 9.557771471772555E-5 0.0 0.09832557401586017 0.0 40-41 0.0 9.557771471772555E-5 0.0 0.11275780893823673 0.0 42-43 0.0 9.557771471772555E-5 0.0 0.1285759207240203 0.0 44-45 0.0 1.1947214339715694E-4 0.0 0.141909011927143 0.0 46-47 0.0 1.4336657207658833E-4 0.0 0.16477598017335887 0.0 48-49 0.0 1.4336657207658833E-4 0.0 0.18943503057053204 0.0 50-51 0.0 1.911554294354511E-4 0.0 0.2079532127970914 0.0 52-53 0.0 2.3894428679431388E-4 0.0 0.2500074072728906 0.0 54-55 0.0 2.3894428679431388E-4 0.0 0.34415145626985033 0.0 56-57 0.0 2.3894428679431388E-4 0.0 0.5332041759815115 0.0 58-59 0.0 2.3894428679431388E-4 0.0 0.7225197344086463 0.0 60-61 0.0 2.3894428679431388E-4 0.0 0.8591719720263145 0.0 62-63 0.0 2.3894428679431388E-4 0.0 1.0445210552926638 0.0 64-65 2.3894428679431388E-5 2.3894428679431388E-4 0.0 1.2961054948583968 0.0 66-67 4.7788857358862776E-5 2.3894428679431388E-4 0.0 1.801377083713653 0.0 68-69 4.7788857358862776E-5 2.3894428679431388E-4 0.0 2.314462150747083 0.0 70-71 4.7788857358862776E-5 2.3894428679431388E-4 0.0 2.590801218424707 0.0 72-73 4.7788857358862776E-5 3.5841643019147084E-4 0.0 2.862528661367201 0.0 74-75 4.7788857358862776E-5 3.823108588709022E-4 0.0 3.1676127267461807 0.0 76-77 4.7788857358862776E-5 3.823108588709022E-4 0.0 3.468276322819466 0.0 78-79 4.7788857358862776E-5 3.823108588709022E-4 0.0 3.6537926670865715 0.0 80-81 4.7788857358862776E-5 3.823108588709022E-4 0.0 3.8632273344617873 0.0 82-83 4.7788857358862776E-5 3.823108588709022E-4 0.0 4.195073159961731 0.0 84-85 4.7788857358862776E-5 3.823108588709022E-4 0.0 4.942729833341138 0.0 86-87 4.7788857358862776E-5 3.823108588709022E-4 0.0 6.020535827784251 0.0 88-89 4.7788857358862776E-5 4.539941449091964E-4 0.0 7.180968756600835 0.0 90-91 4.7788857358862776E-5 4.7788857358862776E-4 0.0 8.42632248494412 0.0 92-93 4.7788857358862776E-5 4.7788857358862776E-4 0.0 9.596934440378144 0.0 94-95 4.7788857358862776E-5 4.7788857358862776E-4 0.0 10.626521477746163 0.0 96-97 4.7788857358862776E-5 4.7788857358862776E-4 0.0 11.610732995051942 0.0 98-99 4.7788857358862776E-5 4.7788857358862776E-4 0.0 12.70877757058653 0.0 100-101 4.7788857358862776E-5 4.7788857358862776E-4 0.0 13.690766906025123 0.0 102-103 4.7788857358862776E-5 4.7788857358862776E-4 0.0 14.36709870979643 0.0 104-105 4.7788857358862776E-5 4.7788857358862776E-4 0.0 15.039201199691476 0.0 106-107 4.7788857358862776E-5 4.7788857358862776E-4 0.0 16.12778358146901 0.0 108-109 4.7788857358862776E-5 4.7788857358862776E-4 0.0 17.212184438227645 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2360 0.0 56.066597 2 ACCTGGG 2575 0.0 50.647774 3 GTACCTG 3795 0.0 35.685352 1 GTACACG 260 0.0 31.030485 1 TATAACG 265 0.0 30.39279 2 CCTGGGG 2310 0.0 29.843487 4 CTGGGGG 1625 0.0 25.102486 5 GTACCGG 170 1.7924885E-5 23.729197 1 GCGTCGG 270 2.590241E-9 23.390924 9 GTACCCG 225 2.6048838E-7 23.051218 1 TAACGCA 350 3.6379788E-12 22.979486 4 ACCGTGA 355 5.456968E-12 22.644905 8 GTACATG 7885 0.0 22.07187 1 TACACGG 320 7.6579454E-10 21.57345 2 GTACTAG 570 0.0 21.231384 1 GAGTACT 5850 0.0 21.066814 12-13 GTATAAC 835 0.0 20.704689 1 TACATGG 7665 0.0 20.489853 2 GGACCGT 170 4.9827574E-4 20.277973 6 AGTACTT 6195 0.0 19.986347 12-13 >>END_MODULE