FastQCFastQC Report
Wed 25 May 2016
SRR1003806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092538
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT145090.69336853141974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60650.28983941988150275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52140.24917110226911052No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44110.21079664980994373No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36230.17313903021115984No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35300.1686946664767856No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35190.16816898904583813No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC32160.15368896526610268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30680.14661621437699102No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29510.14102491806600406No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29050.13882663062749637No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28420.135815932613888No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27530.13156272430894922No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25100.11995003197074557No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22940.10962763878123122No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21100.10083448902720045No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG26950.047.606452
ACCTGGG29150.043.3593833
TATAACG3750.040.5489432
GTACCTG35900.036.81451
TAACGCA4250.034.6421784
GTACCCG1805.456968E-1231.6940141
ATAACGC4550.030.2849353
CCTGGGG22650.029.9847374
GTATAAC7650.025.4795021
ACACGGG3050.023.3685343
GTACACG2704.0017767E-1122.8901211
CTGGGGG13100.022.477755
TACACGG2806.548362E-1122.0620632
TCACGCA3251.8189894E-1221.9199894
TCTAACG1200.00475418319.7992862
GGACCGT1707.966452E-519.5560686
AGTACTT76000.019.4977212-13
GAGTACT73900.019.34482812-13
TATCACG3506.366463E-1119.0073172
TACCCGG3003.7034624E-919.0073172
Produced by FastQC (version 0.11.5)