FastQCFastQC Report
Wed 25 May 2016
SRR1003805_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003805_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411823
Sequences flagged as poor quality0
Sequence length121
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.29575812909915183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.2190261350143144No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6530.15856326625759126No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6010.14593648242084586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.134766635180648No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4390.1065991943140621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA552.1968481E-762.6780434
TAGCGCA350.00894790249.2470364
CCGTGCG350.00895656649.2349789
CGTGCGA256.3291047E-445.97234310-11
TACCTGG4400.040.52922
ACCTGGG4150.040.149193
GTACCGG600.00152861438.4255941
TATAACG959.403564E-636.331782
ATACCGA650.002298870835.361176
CCGCGTA350.00330690732.83738710-11
GTATAAC1552.596862E-729.7488441
ATAACGC1256.186925E-527.578343
TACATGG11750.027.416322
CATGGGG5250.027.3594634
CGTGTGC850.0085457927.0375888
ACATGGG12000.026.8122753
GAGTACT14200.026.1150512-13
GCTCTAG1351.0219717E-425.6170621
GTACATG12750.025.3156851
GTATCAA27450.024.7771591
Produced by FastQC (version 0.11.5)