##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003805_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 411823 Sequences flagged as poor quality 0 Sequence length 121 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.683711206027834 31.0 31.0 34.0 27.0 34.0 2 30.635821700099314 31.0 31.0 34.0 27.0 34.0 3 30.692438256241154 31.0 31.0 34.0 27.0 34.0 4 34.24376249019603 37.0 35.0 37.0 30.0 37.0 5 34.19887184542874 37.0 35.0 37.0 30.0 37.0 6 34.27291093503762 37.0 35.0 37.0 30.0 37.0 7 34.27174295753272 37.0 35.0 37.0 30.0 37.0 8 34.23450365812497 37.0 35.0 37.0 30.0 37.0 9 35.67538966983388 39.0 35.0 39.0 30.0 39.0 10-11 35.54161132331123 39.0 35.0 39.0 30.0 39.0 12-13 35.39389252178727 38.0 35.0 39.0 30.0 39.0 14-15 36.40241681499091 39.0 36.0 41.0 29.0 41.0 16-17 36.417598094326934 39.0 36.0 41.0 30.0 41.0 18-19 36.351029447116844 39.0 36.0 41.0 29.5 41.0 20-21 36.282517003664196 39.0 36.0 41.0 29.0 41.0 22-23 36.177190200644446 39.0 36.0 41.0 27.5 41.0 24-25 35.98717531560889 39.0 35.0 41.0 27.0 41.0 26-27 35.84721712968921 39.0 35.0 41.0 27.0 41.0 28-29 35.661254956619715 39.0 35.0 40.0 27.0 41.0 30-31 35.393964154503266 38.0 34.5 40.0 26.5 41.0 32-33 35.158446711329866 38.0 34.0 40.0 25.5 41.0 34-35 35.148537114245684 38.0 34.0 40.0 25.0 41.0 36-37 35.0286385170328 38.0 34.0 40.0 25.0 41.0 38-39 35.0156547837299 38.0 34.5 40.0 25.0 41.0 40-41 35.0436911003028 38.0 34.5 40.0 25.0 41.0 42-43 34.88707648674309 38.0 34.0 40.0 24.0 41.0 44-45 34.66683016732917 38.0 34.0 40.0 24.0 41.0 46-47 34.39198757718729 38.0 34.0 40.0 22.5 41.0 48-49 34.12854551591339 38.0 33.0 40.0 20.0 41.0 50-51 33.34792131571088 37.5 32.5 39.5 17.5 40.5 52-53 33.46280440869014 38.0 33.0 40.0 18.0 40.5 54-55 33.77266082758855 38.0 33.0 40.0 18.0 41.0 56-57 33.65378330010708 38.0 33.0 40.0 16.5 41.0 58-59 33.41980778149836 38.0 33.0 40.0 13.5 41.0 60-61 33.19989291516015 38.0 33.0 40.0 13.0 41.0 62-63 32.9216787309111 37.0 32.0 40.0 10.0 41.0 64-65 32.64337105989709 37.0 32.0 40.0 8.0 41.0 66-67 32.35139003892449 36.5 31.0 40.0 8.0 41.0 68-69 32.01232811183445 36.0 31.0 39.0 7.0 41.0 70-71 31.642768859437183 35.5 31.0 39.0 2.0 41.0 72-73 31.307794853614297 35.0 31.0 39.0 2.0 40.0 74-75 30.923167234467236 35.0 30.0 38.0 2.0 40.0 76-77 30.483756371062327 35.0 30.0 37.0 2.0 39.5 78-79 29.98143012896317 35.0 29.0 37.0 2.0 39.0 80-81 29.607039917634516 34.0 29.0 36.0 2.0 39.0 82-83 29.261011405385325 34.0 29.0 36.0 2.0 37.5 84-85 28.96168499573846 34.0 29.0 35.5 2.0 37.0 86-87 28.631509167773533 34.0 29.0 35.0 2.0 37.0 88-89 28.295812764221523 34.0 28.5 35.0 2.0 36.0 90-91 27.944913227284538 34.0 27.0 35.0 2.0 36.0 92-93 27.65983080109659 34.0 26.5 35.0 2.0 36.0 94-95 27.40863429191667 34.0 26.0 35.0 2.0 35.0 96-97 27.097563759187807 34.0 25.5 35.0 2.0 35.0 98-99 26.77225652768301 33.5 25.0 35.0 2.0 35.0 100-101 26.402091432484344 33.0 24.5 35.0 2.0 35.0 102-103 26.121196484897638 33.0 24.0 35.0 2.0 35.0 104-105 25.786828321876147 33.0 21.5 35.0 2.0 35.0 106-107 25.404943385872087 32.5 19.5 35.0 2.0 35.0 108-109 25.008611709399425 32.0 18.5 35.0 2.0 35.0 110-111 24.569075792269977 32.0 13.0 35.0 2.0 35.0 112-113 24.09653419065958 32.0 4.5 34.0 2.0 35.0 114-115 23.586024821343152 31.0 2.0 34.0 2.0 35.0 116-117 22.93699841922379 31.0 2.0 34.0 2.0 35.0 118-119 22.212091116814747 31.0 2.0 34.0 2.0 35.0 120-121 20.660595692809775 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.47921077935897216 1101 2 0.5473352233105757 1101 3 0.48290132020001764 1101 4 0.8574770242151715 1101 5 0.645323084035681 1101 6 0.7404284176961653 1101 7 0.5697060340317108 1101 8 0.7402412159131515 1101 9 0.9623617726334217 1101 10-11 0.7363569072090002 1101 12-13 0.8010427897876937 1101 14-15 0.8557821236626921 1101 16-17 0.6030437136610232 1101 18-19 0.7018618898777831 1101 20-21 1.021602561323931 1101 22-23 0.8107137066936048 1101 24-25 0.7923450066704874 1101 26-27 1.0044988034255908 1101 28-29 0.8738212375721446 1101 30-31 0.9087197938780065 1101 32-33 0.8308036210621239 1101 34-35 1.0661562121844739 1101 36-37 0.7001812084735519 1101 38-39 0.7557391207307376 1101 40-41 0.601601713039706 1101 42-43 0.6075850475764994 1101 44-45 0.5854100029851779 1101 46-47 0.8419180071988137 1101 48-49 0.9822407043681594 1101 50-51 0.8177091325878649 1101 52-53 0.6563565342460791 1101 54-55 0.7982563740147768 1101 56-57 0.7011885451539044 1101 58-59 1.0865212574206993 1101 60-61 0.930550007808975 1101 62-63 0.8407816519403681 1101 64-65 0.6637226524065767 1101 66-67 0.902972847039262 1101 68-69 0.8704418988830831 1101 70-71 0.9936990984374638 1101 72-73 1.01974405164502 1101 74-75 0.9515820130251313 1101 76-77 0.5699351511231718 1101 78-79 0.8666395274108218 1101 80-81 0.8288011874585735 1101 82-83 0.719397918343379 1101 84-85 0.7555712630231639 1101 86-87 0.747736983098207 1101 88-89 0.5826571165740653 1101 90-91 0.8688602296501848 1101 92-93 0.9453672131600506 1101 94-95 0.7538493032563132 1101 96-97 0.8364385734140392 1101 98-99 0.9068300586445979 1101 100-101 1.0476776278089908 1101 102-103 1.1359134609306096 1101 104-105 0.9137162917523689 1101 106-107 1.0395990689778714 1101 108-109 1.1181631030883743 1101 110-111 1.1398664482570808 1101 112-113 1.0777948801115613 1101 114-115 0.9623969241074519 1101 116-117 0.9313374716838076 1101 118-119 0.9905396293687971 1101 120-121 1.0365998144930941 1102 1 0.7726598589531157 1102 2 0.7263893553290082 1102 3 0.765738616445315 1102 4 1.1209869513673425 1102 5 1.0784215401539043 1102 6 1.0821247645349388 1102 7 1.0812293768780847 1102 8 1.0751450983104363 1102 9 1.0959956257223382 1102 10-11 1.060575927647939 1102 12-13 1.0167093051688099 1102 14-15 1.1792588192787434 1102 16-17 1.1574449690746036 1102 18-19 0.9049973468498678 1102 20-21 1.2657447338356036 1102 22-23 1.262393360839468 1102 24-25 1.4024456243770516 1102 26-27 1.4513552693440346 1102 28-29 1.2282448011928366 1102 30-31 1.4615287048528813 1102 32-33 1.4443023941107427 1102 34-35 1.3885848066905098 1102 36-37 1.566281964644844 1102 38-39 1.3235772246869786 1102 40-41 1.3622558050572167 1102 42-43 1.4860514737247996 1102 44-45 1.7262094060483193 1102 46-47 1.7042705999509593 1102 48-49 1.5889143199131368 1102 50-51 1.2407112018663895 1102 52-53 1.3734038507226103 1102 54-55 1.4704430398609034 1102 56-57 1.6094171965843174 1102 58-59 1.7637753478997098 1102 60-61 1.6921310442944275 1102 62-63 1.760829806897796 1102 64-65 1.52194438060031 1102 66-67 1.6004120187124506 1102 68-69 1.567452214505746 1102 70-71 1.4157569151563045 1102 72-73 1.2148639390270404 1102 74-75 1.3206770690648852 1102 76-77 1.1891513638049993 1102 78-79 1.284197240281113 1102 80-81 1.5731760576478848 1102 82-83 1.3891806825610153 1102 84-85 1.1626210410949653 1102 86-87 1.5738733554760316 1102 88-89 1.413447442504662 1102 90-91 1.301674063815586 1102 92-93 1.398304588637945 1102 94-95 1.0349634828143053 1102 96-97 1.3594256423464017 1102 98-99 1.5711550046048934 1102 100-101 1.4027204364891865 1102 102-103 1.359537852262715 1102 104-105 1.2521741023172233 1102 106-107 1.4971346274907589 1102 108-109 1.4942602437916221 1102 110-111 1.6781750246088478 1102 112-113 1.5864470028528537 1102 114-115 1.4995997057101036 1102 116-117 1.8393372716791916 1102 118-119 1.755699594598763 1102 120-121 2.142456103484381 1103 1 0.46287093767031706 1103 2 0.5167916876905245 1103 3 0.6774237476406526 1103 4 0.9784796627376053 1103 5 0.7898127937982196 1103 6 0.9370215575378538 1103 7 0.771825295245435 1103 8 0.7081736697390042 1103 9 0.89028367236957 1103 10-11 0.9911590180269414 1103 12-13 1.0959979349064284 1103 14-15 1.1559013848764152 1103 16-17 0.9947650856926771 1103 18-19 0.9666101748382019 1103 20-21 1.280236862115487 1103 22-23 1.1369881130260637 1103 24-25 1.193519676855189 1103 26-27 1.0488608087026279 1103 28-29 1.2382856175193737 1103 30-31 1.0072126698674566 1103 32-33 1.192234755626771 1103 34-35 1.493739325640945 1103 36-37 1.305254559397028 1103 38-39 1.1040705191476192 1103 40-41 1.139514114095114 1103 42-43 1.0952188206635682 1103 44-45 1.1996601349112908 1103 46-47 1.454580804032588 1103 48-49 1.2286670895924345 1103 50-51 1.286861639184174 1103 52-53 1.4054668244835469 1103 54-55 1.140333418869652 1103 56-57 1.0878766718029809 1103 58-59 1.205128117579008 1103 60-61 1.1856529101836415 1103 62-63 1.2762117310960477 1103 64-65 1.062676742116345 1103 66-67 1.3517496280418975 1103 68-69 1.3816936139226605 1103 70-71 1.378726011366222 1103 72-73 1.2792184579774712 1103 74-75 1.0632263402019113 1103 76-77 1.1265251247379453 1103 78-79 1.3781424297854876 1103 80-81 1.5328961742659644 1103 82-83 1.3954232481586821 1103 84-85 1.374746988616831 1103 86-87 1.3664821018317177 1103 88-89 1.4742159556241923 1103 90-91 1.3390839763520326 1103 92-93 1.322124996801211 1103 94-95 1.2993669813565774 1103 96-97 1.4561673333084997 1103 98-99 1.353722934634046 1103 100-101 1.4783530895504136 1103 102-103 1.3327588435163626 1103 104-105 1.6847210410927325 1103 106-107 1.825713053146739 1103 108-109 2.02801534583244 1103 110-111 2.5082041791277945 1103 112-113 2.37501493288201 1103 114-115 2.4364148660599554 1103 116-117 2.269617155061116 1103 118-119 2.1497544050943915 1103 120-121 2.532024616603916 1104 1 0.7044454959742055 1104 2 0.754431263662255 1104 3 0.7945686239657412 1104 4 0.9441333729849504 1104 5 1.3778580234801936 1104 6 1.3165353384565819 1104 7 0.9455835991323625 1104 8 0.9698952245093224 1104 9 1.0503373826169167 1104 10-11 1.203561844882067 1104 12-13 1.2934325638816873 1104 14-15 1.509928240000093 1104 16-17 1.3189177358693627 1104 18-19 1.19438685328344 1104 20-21 1.1313506430342244 1104 22-23 1.2788043674430156 1104 24-25 1.2989599595406958 1104 26-27 1.3253752963351388 1104 28-29 1.4725117659292621 1104 30-31 1.5152070161572055 1104 32-33 1.3784368757681165 1104 34-35 1.3965379122133825 1104 36-37 1.4382029692910265 1104 38-39 1.190836596886136 1104 40-41 1.6251409692187906 1104 42-43 1.6871043330310656 1104 44-45 1.5004318663600529 1104 46-47 1.6467419347746386 1104 48-49 1.779016206200204 1104 50-51 1.9743259501382369 1104 52-53 1.71445763720439 1104 54-55 2.0535334188696552 1104 56-57 2.2644151877040386 1104 58-59 2.0510503790631134 1104 60-61 2.4020204013497164 1104 62-63 2.2988053706720137 1104 64-65 2.148658367558035 1104 66-67 2.01283514040939 1104 68-69 2.0669628718731907 1104 70-71 2.1502086968785896 1104 72-73 1.8941951364227094 1104 74-75 1.9908008985057961 1104 76-77 2.0724431459393564 1104 78-79 2.209134655933898 1104 80-81 2.1357088951140213 1104 82-83 2.097941976073873 1104 84-85 1.6963187200655945 1104 86-87 1.7002319251532754 1104 88-89 1.5905353902531694 1104 90-91 1.6805694887195237 1104 92-93 1.8468514985679967 1104 94-95 2.109711857681667 1104 96-97 2.1698620329551446 1104 98-99 2.380808447001538 1104 100-101 2.0620916054514744 1104 102-103 2.382233048463359 1104 104-105 2.153089945686375 1104 106-107 2.0681356679877254 1104 108-109 2.1806019182706002 1104 110-111 2.284882624357479 1104 112-113 2.0913301272282965 1104 114-115 2.134168929664199 1104 116-117 2.332458144460407 1104 118-119 1.8413628856597626 1104 120-121 1.6843850406321863 1105 1 0.8634042710036525 1105 2 1.1327916876905242 1105 3 1.037423747640652 1105 4 1.1491463294042674 1105 5 1.1220794604648816 1105 6 1.0831548908711923 1105 7 0.6976919619120991 1105 8 1.3065736697390022 1105 9 1.2620170057029014 1105 10-11 1.436492351360279 1105 12-13 0.9933312682397641 1105 14-15 1.2471013848764159 1105 16-17 1.2678317523593492 1105 18-19 0.9842101748382035 1105 20-21 0.8333035287821531 1105 22-23 0.9903214463593883 1105 24-25 1.2620530101885237 1105 26-27 0.956060808702631 1105 28-29 0.9662856175193681 1105 30-31 1.1626793365341186 1105 32-33 0.7773014222934407 1105 34-35 0.6430726589742832 1105 36-37 1.115387892730368 1105 38-39 0.8470038524809596 1105 40-41 0.8989807807617751 1105 42-43 1.2357521539969056 1105 44-45 1.1151268015779578 1105 46-47 1.44844747069925 1105 48-49 1.4916004229257709 1105 50-51 1.2236616391841721 1105 52-53 1.3209334911502069 1105 54-55 1.392866752202984 1105 56-57 1.2953433384696424 1105 58-59 1.3757947842456701 1105 60-61 1.1016529101836454 1105 62-63 1.0882117310960453 1105 64-65 1.2096100754496746 1105 66-67 1.1861496280418997 1105 68-69 1.01609361392266 1105 70-71 0.8192593446995566 1105 72-73 0.8480184579774743 1105 74-75 0.8144263402019121 1105 76-77 0.6827917914046111 1105 78-79 0.8157424297854909 1105 80-81 1.3035628409326314 1105 82-83 1.3676899148253447 1105 84-85 1.0243469886168306 1105 86-87 0.991548768498383 1105 88-89 0.5683492889575277 1105 90-91 0.6665506430186987 1105 92-93 0.5893249968012135 1105 94-95 0.9359003146899099 1105 96-97 0.7580339999751651 1105 98-99 1.1681229346340452 1105 100-101 1.3210197562170798 1105 102-103 1.283158843516361 1105 104-105 1.1508543744260677 1105 106-107 1.2689130531467363 1105 108-109 1.4440153458324367 1105 110-111 1.3828708457944643 1105 112-113 1.6907482662153441 1105 114-115 1.9398815327266234 1105 116-117 2.0509504883944487 1105 118-119 1.7894877384277237 1105 120-121 1.505357949937249 1106 1 -0.07442288949017595 1106 2 0.47869292225842486 1106 3 0.4177200439369493 1106 4 0.5582327491573551 1106 5 0.9957090900945147 1106 6 0.6345129155625457 1106 7 0.27781541870222526 1106 8 0.1990428055414739 1106 9 0.6397454007646317 1106 10-11 0.5608503760516399 1106 12-13 0.5348004040422225 1106 14-15 0.5716075577159216 1106 16-17 0.5662021227297203 1106 18-19 0.7340249896530153 1106 20-21 0.673093652238947 1106 22-23 0.39764243401371147 1106 24-25 0.9308307879663005 1106 26-27 0.6975669815421384 1106 28-29 1.1466436422107336 1106 30-31 1.0645188427069598 1106 32-33 0.9762643852564068 1106 34-35 1.147677597245881 1106 36-37 1.0747088803846907 1106 38-39 0.9064606426044151 1106 40-41 0.8041042375519041 1106 42-43 1.1698509194290025 1106 44-45 1.2222996410841347 1106 46-47 0.5878054953906116 1106 48-49 0.8848596821850307 1106 50-51 0.6500937379496037 1106 52-53 0.47263719485390965 1106 54-55 0.644582801585706 1106 56-57 0.6357507458770542 1106 58-59 0.9733009570851792 1106 60-61 0.7044677249984588 1106 62-63 0.46519938541703 1106 64-65 0.3332273594002899 1106 66-67 0.6329274058196752 1106 68-69 0.46636521886093263 1106 70-71 0.619802554576097 1106 72-73 0.7393888283478454 1106 74-75 0.6409448587204309 1106 76-77 0.34224858152806803 1106 78-79 0.5896313186743782 1106 80-81 0.5590937051301594 1106 82-83 0.7861590506278162 1106 84-85 0.9089272355304132 1106 86-87 0.729116669732953 1106 88-89 0.6879048445130813 1106 90-91 0.8901679269693155 1106 92-93 0.7255595647024471 1106 94-95 0.4874929072825047 1106 96-97 0.6922685678763969 1106 98-99 0.9299130580908361 1106 100-101 0.7621308673281924 1106 102-103 0.6796526706768553 1106 104-105 0.9729161028211308 1106 106-107 0.809159966726984 1106 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2.6333769100683213 2206 114-115 2.410667337542847 2206 116-117 2.329784455441345 2206 118-119 2.1352570666913486 2206 120-121 2.093613970216083 2207 1 0.6638919493434692 2207 2 0.7561224269901352 2207 3 0.8081941756562152 2207 4 0.9419816082628927 2207 5 1.006805789907169 2207 6 0.9172145536468079 2207 7 1.1605194586695617 2207 8 1.1504647203226668 2207 9 1.0345669408520592 2207 10-11 0.9267232203615734 2207 12-13 1.2885893745951407 2207 14-15 1.4125488556935366 2207 16-17 1.2091261751868458 2207 18-19 1.0947938324257578 2207 20-21 1.4421465897419523 2207 22-23 1.2470503698353994 2207 24-25 1.4233188986450642 2207 26-27 1.332768979908856 2207 28-29 1.7213362011769604 2207 30-31 1.69242512122932 2207 32-33 1.5976075182726888 2207 34-35 1.7757159793374413 2207 36-37 1.8540779057005352 2207 38-39 1.5858572895756353 2207 40-41 1.737878316429743 2207 42-43 1.7127845794184324 2207 44-45 1.9448881504754993 2207 46-47 1.96129831376021 2207 48-49 2.0782593074912725 2207 50-51 2.1108289543592704 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0.086149496736045 2316 102-103 -0.15149983911836884 2316 104-105 0.1030539752244728 2316 106-107 0.17801484955392155 2316 108-109 0.12003929792824763 2316 110-111 0.18155747254096966 2316 112-113 0.3035346933610512 2316 114-115 0.7184244468982826 2316 116-117 0.7879764364982442 2316 118-119 1.5165496146752275 2316 120-121 1.0504797064242766 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6185.0 3 957.0 4 880.0 5 946.0 6 1187.0 7 1177.0 8 1381.0 9 1712.0 10 2199.0 11 2726.0 12 3144.0 13 3599.0 14 4460.0 15 4539.0 16 3154.0 17 3053.0 18 3223.0 19 3365.0 20 3786.0 21 4020.0 22 4422.0 23 5060.0 24 5800.0 25 6423.0 26 7417.0 27 8606.0 28 9700.0 29 11416.0 30 13675.0 31 16070.0 32 19334.0 33 23711.0 34 30572.0 35 40322.0 36 55454.0 37 67636.0 38 30348.0 39 164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.493652330649546 20.40742159012471 15.08269653384326 22.016229545382483 2 14.16593980614875 20.347030978958614 42.572302204102435 22.9147270107902 3 17.663434017975842 27.012978745804126 31.196488075272317 24.12709916094771 4 11.65822896368034 17.14779526248274 40.872102701697 30.32187307213991 5 11.598294030084613 37.2877330774052 37.074242008774675 14.039730883735507 6 28.00281073563905 38.32198102998281 19.513066982670395 14.162141251707741 7 25.27377556891212 32.99555642604449 24.435984380164278 17.294683624879116 8 23.85971097091348 35.51072169138078 21.939133989928294 18.690433347777443 9 24.028595561322543 17.652862616444896 23.644954768579158 34.6735870536534 10-11 22.72967633428565 26.97128602099996 29.920534081478273 20.37850356323612 12-13 23.84170085040072 24.76334573373534 30.110208113755633 21.284745302108313 14-15 21.058687865559715 26.41994471770037 28.554482934221898 23.966884482518015 16-17 19.689202276865135 29.743111919748955 29.269520053579058 21.298165749806852 18-19 19.416788516033908 28.68566782025565 31.917798824857762 19.97974483885268 20-21 20.26864688327735 27.710868455115108 31.518801485652286 20.50168317595526 22-23 20.13144829426983 27.4918671265398 31.53387768794988 20.842806891240496 24-25 20.12877380840152 27.71171219702711 31.556071945845947 20.603442048725423 26-27 19.95222475668541 27.58181602246 31.73931515621231 20.72664406464228 28-29 19.821655551773034 27.866993541687584 31.8070049545567 20.504345951982682 30-31 20.126948436841744 27.92473718758879 31.370200550607922 20.578113824961548 32-33 19.939452723157864 27.958415385670477 31.385386356288393 20.716745534883266 34-35 20.020478049784195 27.66312628294002 31.628176424767663 20.688219242508122 36-37 20.386383846004982 27.896776500963938 31.29162879910637 20.425210853924714 38-39 20.326628088545974 28.13251278282968 31.31502403478154 20.225835093842807 40-41 19.975846682777043 27.96211897590915 31.494480379104196 20.56755396220961 42-43 19.84622412625396 28.22465778254602 31.063804250514078 20.865313840685943 44-45 20.019545601504866 28.21473708961585 30.7403570133929 21.025360295486387 46-47 20.073233158210563 28.0350735393169 30.6519955423295 21.239697760143038 48-49 20.414529092844084 27.982673252169825 30.603362231657687 20.999435423328407 50-51 20.16456820757896 28.13430138161321 30.60221180651288 21.098918604294948 52-53 19.819691827589036 28.36332549899509 30.452264629983674 21.364718043432198 54-55 19.83136420090295 28.64799849099866 30.443612098467238 21.077025209631152 56-57 20.01124571394971 28.282113067201102 30.22046744439313 21.48617377445606 58-59 20.05651792754539 27.77459435898189 30.358183590210743 21.810704123261974 60-61 20.231701656254096 27.879277043801387 30.592628609851303 21.296392690093217 62-63 19.630452171803974 27.833193891338144 30.728401700743184 21.8079522361147 64-65 19.638331828832285 27.556988335932015 31.080950322069377 21.723729513166322 66-67 19.732646719085515 27.313985151395258 31.02405395572857 21.929314173790658 68-69 19.75611907603116 27.644820378869372 30.544535896047382 22.05452464905209 70-71 19.869575077072057 27.4197561887484 30.546399524390317 22.164269209789225 72-73 19.266973861473037 27.370003027817923 31.11469055578234 22.2483325549267 74-75 19.58150060559699 27.897793861649284 30.96684662949723 21.553858903256494 76-77 19.780508077704848 28.097883785990476 30.220350078827806 21.901258057476873 78-79 19.734052261082482 28.31721176235698 29.795774380482143 22.152961596078395 80-81 19.786103462130196 27.835580663811772 30.08870912422707 22.28960674983096 82-83 19.907660411852206 26.896355674056938 30.675466047801912 22.520517866288944 84-85 20.297143109148884 26.73981725012771 30.268514174201616 22.694525466521785 86-87 20.81520407663286 26.702435200156792 29.878117497182615 22.60424322602773 88-89 19.97388346291562 26.23009257160917 30.642096868833 23.153927096642214 90-91 19.712198133918772 26.310128195076054 31.29116355653128 22.68651011447389 92-93 19.7117569595443 27.256791106483323 30.654457477107773 22.376994456864608 94-95 19.610780643643917 27.490102308807966 30.084571361373524 22.814545686174593 96-97 19.207366000605177 27.894954127878417 29.91310696798078 22.98457290353563 98-99 19.993016160848843 27.54524180011517 30.27151206243797 22.190229976598015 100-101 19.93555540581104 26.88478997310725 30.658351762118585 22.52130285896313 102-103 20.865606881539183 26.83819881444859 30.06202156238477 22.234172741627457 104-105 20.656113613340796 27.13505793236753 29.4410834130549 22.767745041236772 106-107 21.087490430255702 26.525677537896186 29.72947481243301 22.657357219415097 108-109 21.710008942023837 26.283425407597043 29.166554380979214 22.840011269399906 110-111 21.734896488535405 26.148878651319297 28.405907521347213 23.710317338798088 112-113 21.87670732812795 26.49785504462634 28.6762526122886 22.949185014957113 114-115 21.756887533227985 25.68740582852523 29.587054255019417 22.968652383227372 116-117 21.327702305339923 26.119344467968318 29.50494491492198 23.04800831176978 118-119 21.925583788684424 26.257504104285612 29.21381490284482 22.603097204185147 120-121 22.479337223133488 27.008794313035406 28.56026352313974 21.951604940691368 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1024.0 1 1034.0 2 682.5 3 311.5 4 278.5 5 242.5 6 246.5 7 294.0 8 342.0 9 386.0 10 377.0 11 354.5 12 370.0 13 390.0 14 434.0 15 477.5 16 526.5 17 575.0 18 646.5 19 735.5 20 804.5 21 906.5 22 1047.0 23 1287.0 24 1566.0 25 1930.0 26 2504.5 27 3223.5 28 4337.5 29 5934.5 30 7693.5 31 9344.5 32 10804.5 33 11892.5 34 12641.5 35 13429.5 36 14006.5 37 14707.5 38 15408.0 39 15346.5 40 15274.5 41 15571.0 42 15746.0 43 16010.0 44 16167.5 45 16191.0 46 16162.5 47 16102.0 48 16805.0 49 16432.5 50 14817.0 51 13196.0 52 11342.5 53 9955.5 54 8842.0 55 7646.5 56 6432.0 57 5171.5 58 4034.0 59 3187.5 60 2474.0 61 1821.0 62 1394.5 63 1041.0 64 691.5 65 459.5 66 279.5 67 179.0 68 112.0 69 62.5 70 47.5 71 36.5 72 21.0 73 13.5 74 15.5 75 12.0 76 5.5 77 7.0 78 7.5 79 4.0 80 3.5 81 4.0 82 3.0 83 2.0 84 2.0 85 1.5 86 0.5 87 0.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4443656619470015 2 0.8923736653853719 3 0.8221978859849984 4 0.8107852159787093 5 0.8195268355579945 6 0.8229263542832722 7 0.8178270761953557 8 0.8139419119378957 9 0.8178270761953557 10-11 0.8124849753413481 12-13 0.8179484875784013 14-15 0.8192840127919033 16-17 0.8388312454622496 18-19 0.8576500098343219 20-21 0.8541290797259987 22-23 0.8376171316317932 24-25 0.8334891446082417 26-27 0.838345599930067 28-29 0.8527935545124968 30-31 0.8612923513256909 32-33 0.8612923513256909 34-35 0.8695483253727937 36-37 0.8746476034607101 38-39 0.8645704586679228 40-41 0.8735549010132994 42-43 0.8660273952644704 44-45 0.8612923513256909 46-47 0.8591069464308696 48-49 0.8626278765391928 50-51 0.919205581038456 52-53 0.8686984456914743 54-55 0.8756188945250751 56-57 0.8905524946396873 58-59 0.8394383023774777 60-61 0.8699125595219305 62-63 0.8859388620839536 64-65 0.8750118376098469 66-67 0.8830249888908583 68-69 0.901843753262931 70-71 0.9532007682912319 72-73 0.9563574642504182 74-75 0.9618209764874714 76-77 0.9644920269144754 78-79 0.8796254701655808 80-81 0.8826607547417216 82-83 0.9139848915674937 84-85 0.89103814017187 86-87 0.8846033368704517 88-89 0.8869101531483187 90-91 0.8972301207071971 92-93 0.8888527352770487 94-95 0.8855746279348167 96-97 0.8929807223006001 98-99 0.9076714996491211 100-101 0.9034221012425242 102-103 0.8883670897448662 104-105 0.8818108750604021 106-107 0.8827821661247672 108-109 0.8833892230399953 110-111 0.8818108750604021 112-113 0.8877600328296379 114-115 0.8882456783618206 116-117 0.9050004492221172 118-119 0.9016009304968396 120-121 0.8890955580431398 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 411823.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.46393806700563 #Duplication Level Percentage of deduplicated Percentage of total 1 90.45147780962327 72.78082108542661 2 6.441231806830638 10.365737543600943 3 1.3806357543968293 3.3327416950464013 4 0.5638358584882487 1.8147381438942154 5 0.3093754735129617 1.2446784470098744 6 0.1984518580742764 0.958093081042246 7 0.14087731854600913 0.793488068417233 8 0.09022805468537702 0.5808083683288459 9 0.07604560921886314 0.5507036271408883 >10 0.3226649167995379 4.359486828529116 >50 0.014559348401283178 0.7997945227590928 >100 0.009099592648064871 1.4599849841821295 >500 0.0012132790197419822 0.6616548465593032 >1k 3.0331975493549555E-4 0.29726875806316166 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1218 0.29575812909915183 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 902 0.2190261350143144 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 653 0.15856326625759126 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 601 0.14593648242084586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 555 0.134766635180648 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 439 0.1065991943140621 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.4282276609125767E-4 0.0 6 0.0 0.0 0.0 2.4282276609125767E-4 0.0 7 0.0 0.0 0.0 2.4282276609125767E-4 0.0 8 0.0 0.0 0.0 2.4282276609125767E-4 0.0 9 0.0 0.0 0.0 2.4282276609125767E-4 0.0 10-11 0.0 0.0 0.0 3.642341491368865E-4 0.0 12-13 0.0 0.0 0.0 4.8564553218251533E-4 0.0 14-15 0.0 1.2141138304562883E-4 0.0 7.28468298273773E-4 0.0 16-17 0.0 2.4282276609125767E-4 0.0 0.0012141138304562883 0.0 18-19 0.0 2.4282276609125767E-4 0.0 0.001456936596547546 0.0 20-21 0.0 2.4282276609125767E-4 0.0 0.0019425821287300613 0.0 22-23 0.0 2.4282276609125767E-4 0.0 0.0036423414913688643 0.0 24-25 0.0 2.4282276609125767E-4 0.0 0.006313391918372699 0.0 26-27 0.0 2.4282276609125767E-4 0.0 0.009105853728422163 0.0 28-29 0.0 2.4282276609125767E-4 0.0 0.02051852373471127 0.0 30-31 0.0 2.4282276609125767E-4 0.0 0.042251161299878826 0.0 32-33 0.0 2.4282276609125767E-4 0.0 0.08049574695925191 0.0 34-35 0.0 2.4282276609125767E-4 0.0 0.10866318782583781 0.0 36-37 0.0 2.4282276609125767E-4 0.0 0.13634498316024118 0.0 38-39 0.0 3.642341491368865E-4 0.0 0.2079776991571622 0.0 40-41 0.0 4.8564553218251533E-4 0.0 0.33630953103639183 0.0 42-43 0.0 4.8564553218251533E-4 0.0 0.5367597244447251 0.0 44-45 0.0 4.8564553218251533E-4 0.0 0.7057643696442404 0.0 46-47 0.0 4.8564553218251533E-4 0.0 1.1024153580543097 0.0 48-49 0.0 4.8564553218251533E-4 0.0 1.4149282580137583 0.0 50-51 0.0 4.8564553218251533E-4 0.0 1.5957098073686997 0.0 52-53 0.0 4.8564553218251533E-4 0.0 1.8335547067550866 0.0 54-55 0.0 4.8564553218251533E-4 0.0 2.0442034563392526 0.0 56-57 0.0 4.8564553218251533E-4 0.0 2.5620230050288595 0.0 58-59 0.0 4.8564553218251533E-4 0.0 3.2914625943669975 0.0 60-61 0.0 4.8564553218251533E-4 0.0 3.854204354783487 0.0 62-63 0.0 4.8564553218251533E-4 0.0 4.344220696755645 0.0 64-65 0.0 4.8564553218251533E-4 0.0 5.117975440905438 0.0 66-67 0.0 4.8564553218251533E-4 0.0 6.15725687977602 0.0 68-69 0.0 7.28468298273773E-4 0.0 7.056429582611948 0.0 70-71 0.0 7.28468298273773E-4 0.0 7.489261163169614 0.0 72-73 0.0 7.28468298273773E-4 0.0 7.829941503995649 0.0 74-75 0.0 7.28468298273773E-4 0.0 8.293611575846905 0.0 76-77 0.0 7.28468298273773E-4 0.0 8.761531046104759 0.0 78-79 0.0 7.28468298273773E-4 0.0 9.188656291659282 0.0 80-81 0.0 7.28468298273773E-4 0.0 10.05164840234761 0.0 82-83 0.0 7.28468298273773E-4 0.0 11.23990160821518 0.0 84-85 0.0 7.28468298273773E-4 0.0 13.01615014217273 0.0 86-87 0.0 8.498796813194018E-4 0.0 14.14551882726317 0.0 88-89 0.0 9.712910643650307E-4 0.0 15.43539336073993 0.0 90-91 0.0 9.712910643650307E-4 0.0 16.48863710866076 0.0 92-93 0.0 9.712910643650307E-4 0.0 17.486760088678874 0.0 94-95 0.0 9.712910643650307E-4 0.0 18.376341292254196 0.0 96-97 0.0 9.712910643650307E-4 0.0 19.471593378708814 0.0 98-99 0.0 9.712910643650307E-4 0.0 20.4108318379498 0.0 100-101 0.0 9.712910643650307E-4 0.0 20.869888277245323 0.0 102-103 0.0 9.712910643650307E-4 0.0 21.168317456771476 0.0 104-105 0.0 9.712910643650307E-4 0.0 21.576016881038697 0.0 106-107 0.0 9.712910643650307E-4 0.0 22.15867010827467 0.0 108-109 0.0 9.712910643650307E-4 0.0 23.012070719702397 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 55 2.1968481E-7 62.678043 4 TAGCGCA 35 0.008947902 49.247036 4 CCGTGCG 35 0.008956566 49.234978 9 CGTGCGA 25 6.3291047E-4 45.972343 10-11 TACCTGG 440 0.0 40.5292 2 ACCTGGG 415 0.0 40.14919 3 GTACCGG 60 0.001528614 38.425594 1 TATAACG 95 9.403564E-6 36.33178 2 ATACCGA 65 0.0022988708 35.36117 6 CCGCGTA 35 0.003306907 32.837387 10-11 GTATAAC 155 2.596862E-7 29.748844 1 ATAACGC 125 6.186925E-5 27.57834 3 TACATGG 1175 0.0 27.41632 2 CATGGGG 525 0.0 27.359463 4 CGTGTGC 85 0.00854579 27.037588 8 ACATGGG 1200 0.0 26.812275 3 GAGTACT 1420 0.0 26.11505 12-13 GCTCTAG 135 1.0219717E-4 25.617062 1 GTACATG 1275 0.0 25.315685 1 GTATCAA 2745 0.0 24.777159 1 >>END_MODULE