FastQCFastQC Report
Wed 25 May 2016
SRR1003805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411823
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.3329100123111143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.27778924440839875No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8010.19450103563909737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.16779053136905903No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4610.11194129516806978No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA4410.10708483984624463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA654.418689E-851.14114
TATAACG502.8218892E-547.5054632
ACACGGG652.5832596E-643.8512043
TACACGG853.629848E-739.1221472
ATAACGC756.906015E-638.0043753
TAACCGT500.00161562637.9951447
TACGTGG651.325806E-436.5426672
GTACACG958.632942E-735.02531
TACCTGG4900.033.9324762
AACCGTG600.003950504631.6626198
CACGCAG902.4113368E-531.6626195
CTAACCG600.003950504631.6626196
TCACGCA753.0795424E-431.6587734
ACCTGGG5300.031.3715323
GTCTTAC856.3731516E-427.9613721
GTACGTG1056.8614085E-527.1624761
GGCTAGA908.90012E-426.4079651
ACGTGGG908.9317525E-426.3919263
ATGCCGC500.001655649523.74408194-95
GTACCTG7150.023.2685551
Produced by FastQC (version 0.11.5)