##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003804_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1632341 Sequences flagged as poor quality 0 Sequence length 121 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.866498482853768 31.0 31.0 34.0 28.0 34.0 2 30.751829427797254 31.0 31.0 34.0 27.0 34.0 3 30.84875770442573 31.0 31.0 34.0 27.0 34.0 4 34.26160220199088 37.0 35.0 37.0 30.0 37.0 5 34.25772066008267 37.0 35.0 37.0 30.0 37.0 6 34.393343057608675 37.0 35.0 37.0 30.0 37.0 7 34.4458161621867 37.0 35.0 37.0 30.0 37.0 8 34.42127839709963 37.0 35.0 37.0 30.0 37.0 9 35.87469468695573 39.0 35.0 39.0 30.0 39.0 10-11 35.684963803519 39.0 35.0 39.0 30.0 39.0 12-13 35.56394282812231 39.0 35.0 39.0 30.0 39.0 14-15 36.47249716817748 39.0 36.0 41.0 30.0 41.0 16-17 36.616479032261026 39.0 36.0 41.0 30.0 41.0 18-19 36.64303445174752 39.0 36.0 41.0 30.0 41.0 20-21 36.48838845559843 39.0 36.0 41.0 29.5 41.0 22-23 36.39860268167007 39.0 36.0 41.0 29.5 41.0 24-25 36.13217612006315 39.0 35.5 41.0 28.0 41.0 26-27 35.93536123885879 39.0 35.5 40.5 27.0 41.0 28-29 35.8072960858056 39.0 35.0 40.0 27.0 41.0 30-31 35.61368029106663 38.0 35.0 40.0 27.0 41.0 32-33 35.34632929026472 38.0 34.5 40.0 26.5 41.0 34-35 35.251557119498926 38.0 34.5 40.0 25.0 41.0 36-37 35.07782626301734 38.0 34.5 40.0 25.0 41.0 38-39 35.07044912797019 38.0 34.5 40.0 25.0 41.0 40-41 35.324843277231906 38.5 35.0 40.0 25.5 41.0 42-43 35.156699794957056 38.0 35.0 40.0 24.5 41.0 44-45 34.91346967330968 38.0 34.0 40.0 24.0 41.0 46-47 34.64014810630867 38.0 34.0 40.0 23.5 41.0 48-49 34.33764146094474 38.0 33.0 40.0 23.0 41.0 50-51 33.50172267926861 37.5 32.5 39.5 21.0 40.5 52-53 33.71126835630545 38.0 33.0 39.5 22.0 40.5 54-55 34.0821559343299 38.0 33.0 40.0 22.0 41.0 56-57 33.70882554564273 38.0 33.0 40.0 19.5 41.0 58-59 33.357343532999536 38.0 32.0 40.0 18.0 41.0 60-61 32.89391309781473 37.0 31.5 40.0 13.0 41.0 62-63 32.76759359717118 37.0 31.5 40.0 13.5 41.0 64-65 32.50093883569671 36.5 31.0 39.5 13.0 41.0 66-67 31.900363955815607 36.0 31.0 39.0 8.0 40.5 68-69 31.447695058814304 35.5 30.0 39.0 7.5 40.0 70-71 30.878305145799807 35.0 29.5 37.5 6.5 40.0 72-73 30.44571753083455 35.0 29.0 37.0 2.0 39.5 74-75 30.130547171209937 34.5 29.0 37.0 2.0 39.0 76-77 29.739327138140865 34.0 29.0 36.0 2.0 39.0 78-79 29.103483892152436 34.0 28.5 36.0 2.0 38.0 80-81 28.488706403870268 34.0 26.0 35.5 2.0 37.0 82-83 28.02948954905868 33.5 26.0 35.0 2.0 37.0 84-85 27.739132938522037 33.0 26.0 35.0 2.0 36.0 86-87 27.767586552074597 34.0 26.0 35.0 2.0 36.0 88-89 27.340596725806677 33.5 25.5 35.0 2.0 36.0 90-91 26.868612318136957 33.0 25.0 35.0 2.0 35.0 92-93 26.59031783187459 33.0 25.0 35.0 2.0 35.0 94-95 26.339481762695417 33.0 24.0 35.0 2.0 35.0 96-97 26.04438533370172 32.0 23.0 35.0 2.0 35.0 98-99 25.857390704515787 32.0 23.0 35.0 2.0 35.0 100-101 25.60796365465304 32.0 20.0 35.0 2.0 35.0 102-103 25.037416814256336 32.0 18.5 34.5 2.0 35.0 104-105 24.56748681801168 31.5 16.5 34.0 2.0 35.0 106-107 24.012427244062362 31.0 10.0 34.0 2.0 35.0 108-109 23.812263185204564 31.0 7.0 34.0 2.0 35.0 110-111 23.52286072579198 31.0 2.0 34.0 2.0 35.0 112-113 22.78001869707371 31.0 2.0 34.0 2.0 35.0 114-115 22.207129208909166 30.0 2.0 34.0 2.0 35.0 116-117 21.515017389136215 29.5 2.0 34.0 2.0 35.0 118-119 20.774194546360107 29.0 2.0 34.0 2.0 35.0 120-121 19.164032821573436 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.47330254962142604 1101 2 0.28240039857342936 1101 3 0.341121358123182 1101 4 0.5405914482014893 1101 5 0.6600595701055454 1101 6 0.5980776760119468 1101 7 0.47888014916159705 1101 8 0.43349391885800515 1101 9 0.5131952986439359 1101 10-11 0.6367243063899863 1101 12-13 0.7297747584842611 1101 14-15 0.7248470906501439 1101 16-17 0.6903906190258553 1101 18-19 0.7143645932824185 1101 20-21 0.9224912118631892 1101 22-23 0.6591128514060642 1101 24-25 0.6634782294531618 1101 26-27 0.6953992420033472 1101 28-29 0.8322137103663749 1101 30-31 0.9120417616332901 1101 32-33 0.6798348119136861 1101 34-35 0.8399029849646311 1101 36-37 0.7275992420411228 1101 38-39 0.770529308562601 1101 40-41 0.6602926863747669 1101 42-43 0.7771170000872374 1101 44-45 1.015710293694653 1101 46-47 0.6715276351496513 1101 48-49 0.9288585686001696 1101 50-51 0.5004652540942303 1101 52-53 0.6093969965886217 1101 54-55 0.8132834148772119 1101 56-57 0.8913179228473496 1101 58-59 0.785931071521091 1101 60-61 0.7592861920218255 1101 62-63 0.6645964386676866 1101 64-65 0.677027009519314 1101 66-67 0.7331412765550418 1101 68-69 0.5526409700530053 1101 70-71 0.18240696631784203 1101 72-73 0.1401117111106096 1101 74-75 0.19914931041612505 1101 76-77 0.2582350340009292 1101 78-79 0.7053880982602081 1101 80-81 0.7714187332608589 1101 82-83 0.9859149732210035 1101 84-85 0.7962699016622317 1101 86-87 0.736043461361497 1101 88-89 0.8825991808941147 1101 90-91 0.9900570004919445 1101 92-93 0.9631045684125574 1101 94-95 1.0466014925304314 1101 96-97 1.0577794039844228 1101 98-99 0.9937089981747995 1101 100-101 0.7370262801437697 1101 102-103 0.7216206427208753 1101 104-105 0.8126742375588023 1101 106-107 0.7516799668766758 1101 108-109 0.6040923292460718 1101 110-111 0.39360539036904285 1101 112-113 0.5510435765829804 1101 114-115 0.6397166284936375 1101 116-117 0.6204177231074333 1101 118-119 0.5264032613903282 1101 120-121 0.7059636923340769 1102 1 0.3761414454070682 1102 2 0.3766972046183348 1102 3 0.5168168739566177 1102 4 0.71257534567345 1102 5 0.7316667373092045 1102 6 0.6927622630796932 1102 7 0.5076468419003888 1102 8 0.6411031526962745 1102 9 0.5978971794249546 1102 10-11 0.6866282222320379 1102 12-13 0.6683416047854891 1102 14-15 0.7312635605798903 1102 16-17 0.5423547112494234 1102 18-19 0.6314477681498403 1102 20-21 0.7738561235647978 1102 22-23 0.567929057265836 1102 24-25 0.8795223463615329 1102 26-27 0.7418845997535897 1102 28-29 0.8049500637608986 1102 30-31 0.8316403465626436 1102 32-33 0.6437479271500948 1102 34-35 0.7197395199072645 1102 36-37 0.7587688280683764 1102 38-39 0.49417792327159304 1102 40-41 0.6862550707544557 1102 42-43 0.7539417021115256 1102 44-45 0.7587947458570738 1102 46-47 0.8451969879629289 1102 48-49 0.7209300110106724 1102 50-51 0.3212824358647879 1102 52-53 0.499598995770576 1102 54-55 0.6241793153343096 1102 56-57 0.6678504256997329 1102 58-59 0.7942313363086484 1102 60-61 0.7771218228118073 1102 62-63 0.6851888739239058 1102 64-65 0.6021459486468785 1102 66-67 0.6014765593180194 1102 68-69 0.43521163388761863 1102 70-71 0.36395966908842325 1102 72-73 0.4685576449352489 1102 74-75 0.7091782289490993 1102 76-77 0.5112835353320442 1102 78-79 0.6985753798463499 1102 80-81 0.6850179066070119 1102 82-83 0.4917591001755177 1102 84-85 0.6454631248228928 1102 86-87 0.6502875969269866 1102 88-89 0.4292393927241598 1102 90-91 0.6723468029417674 1102 92-93 0.5703197065111105 1102 94-95 0.4502535516304391 1102 96-97 0.40571140596494715 1102 98-99 0.6137554974536314 1102 100-101 0.6046596247192078 1102 102-103 0.7043847665180323 1102 104-105 0.5446835374145707 1102 106-107 0.43786322283080636 1102 108-109 0.5334594941534689 1102 110-111 0.5641790967490579 1102 112-113 0.4440039589103826 1102 114-115 0.217461700502934 1102 116-117 0.3864779855987308 1102 118-119 0.30530335252317187 1102 120-121 0.49544696192225857 1103 1 0.5184526420297146 1103 2 0.5503649869985416 1103 3 0.5034617033796529 1103 4 0.7585248284964976 1103 5 0.9312300984426187 1103 6 0.8347404947399895 1103 7 0.7705008954387367 1103 8 0.6961791192725713 1103 9 0.8053356230443143 1103 10-11 0.7960732011235834 1103 12-13 0.877478901154511 1103 14-15 0.9594259724292442 1103 16-17 0.743365849833765 1103 18-19 0.6261041098284963 1103 20-21 0.8115331602720346 1103 22-23 0.8624034925849244 1103 24-25 0.7684527719492635 1103 26-27 0.9851102574547923 1103 28-29 0.8393465846427546 1103 30-31 0.8884860839273898 1103 32-33 0.9954133084935606 1103 34-35 0.6915841044563606 1103 36-37 0.6710085501205469 1103 38-39 0.7087566685311373 1103 40-41 0.7420364015012382 1103 42-43 0.8190644968546863 1103 44-45 0.6940745620426156 1103 46-47 0.8580935764236486 1103 48-49 0.7549070660039519 1103 50-51 0.5370804625624146 1103 52-53 0.6155813139635953 1103 54-55 0.43710961371481005 1103 56-57 0.5274312778292654 1103 58-59 0.7196052063505434 1103 60-61 0.5765920046386626 1103 62-63 0.7290403912678158 1103 64-65 0.7280843008360378 1103 66-67 0.721204812842057 1103 68-69 0.7798375253321339 1103 70-71 0.5374498383535595 1103 72-73 0.5281321367025562 1103 74-75 0.4467401431001079 1103 76-77 0.657216004186143 1103 78-79 0.7310315132231437 1103 80-81 0.7582719474246389 1103 82-83 0.6607400906429248 1103 84-85 0.8951674474840665 1103 86-87 0.8639914743628054 1103 88-89 0.8991264167901782 1103 90-91 0.9156869471629818 1103 92-93 0.8854242947174065 1103 94-95 0.8384560337618012 1103 96-97 0.8415972056612411 1103 98-99 1.0343619579915604 1103 100-101 0.9936740070882841 1103 102-103 0.7935624511871744 1103 104-105 1.008674823718323 1103 106-107 0.8799785202970014 1103 108-109 0.9613366437571393 1103 110-111 1.0257340463034375 1103 112-113 0.8533913765238168 1103 114-115 0.6525856878140139 1103 116-117 0.3990164856663121 1103 118-119 0.2669626120179984 1103 120-121 0.6972378809150932 1104 1 0.46714718792415155 1104 2 0.5959070471001304 1104 3 0.6472682550480151 1104 4 0.8686345032237384 1104 5 1.1511470234726247 1104 6 0.8834481570195791 1104 7 0.7344515642384621 1104 8 0.6766442004650344 1104 9 0.7695503185606896 1104 10-11 0.9514094291230535 1104 12-13 1.0411615335654432 1104 14-15 1.0590950385562152 1104 16-17 1.0373065895978897 1104 18-19 0.8770725475922703 1104 20-21 1.002955927870076 1104 22-23 0.9384914389667713 1104 24-25 0.9824455762867856 1104 26-27 1.0644502858079363 1104 28-29 1.0234950254239834 1104 30-31 0.9994124745020727 1104 32-33 1.0414414563920147 1104 34-35 1.028563747554763 1104 36-37 0.9707775862089534 1104 38-39 0.8175273506239336 1104 40-41 0.8698433419039233 1104 42-43 0.7471579928946284 1104 44-45 0.7490481865551573 1104 46-47 0.8975735559267477 1104 48-49 0.9494765767288058 1104 50-51 0.7174885248577709 1104 52-53 0.9715676525348798 1104 54-55 0.7917209405628896 1104 56-57 0.825650303791555 1104 58-59 0.7052271336844171 1104 60-61 0.595244115968903 1104 62-63 0.6019592694838778 1104 64-65 0.48921165074177964 1104 66-67 0.7106148609445277 1104 68-69 0.7133202925767819 1104 70-71 0.592271401147066 1104 72-73 0.3171719017765433 1104 74-75 0.34493983428739483 1104 76-77 0.45766430592009044 1104 78-79 0.6097452171951687 1104 80-81 0.5633921968126536 1104 82-83 0.5883549745459256 1104 84-85 0.352195825089165 1104 86-87 0.51019873227715 1104 88-89 0.6322631969011177 1104 90-91 0.7834972312244659 1104 92-93 0.7963939421788773 1104 94-95 0.6667097128058934 1104 96-97 0.5511380846085601 1104 98-99 0.6270664274847526 1104 100-101 0.8487979263457213 1104 102-103 0.9180070436345851 1104 104-105 0.9115354189711447 1104 106-107 0.757368692658531 1104 108-109 0.7619183180603066 1104 110-111 0.8818727863964853 1104 112-113 0.9047097431328979 1104 114-115 0.6663617999543483 1104 116-117 0.5398832340668598 1104 118-119 0.5624000128805555 1104 120-121 0.6521531952801851 1105 1 0.6353034745166575 1105 2 0.8223247017644546 1105 3 0.8121525617420033 1105 4 0.9947917412213982 1105 5 1.173877068359431 1105 6 0.9974155165043541 1105 7 1.0542243247771026 1105 8 0.9171697908241327 1105 9 1.1576931269135358 1105 10-11 1.1245354354169663 1105 12-13 1.2295222197968982 1105 14-15 1.43505101777167 1105 16-17 1.2422589278590124 1105 18-19 1.1258573714603486 1105 20-21 1.3459153772748351 1105 22-23 1.3267127687384388 1105 24-25 1.3505684722176952 1105 26-27 1.4055480914773284 1105 28-29 1.528855065187841 1105 30-31 1.5081885771562469 1105 32-33 1.3919717427893659 1105 34-35 1.4738953061515545 1105 36-37 1.5590524325204314 1105 38-39 1.4240527092304944 1105 40-41 1.4903882043160976 1105 42-43 1.6533027894402963 1105 44-45 1.6950231633799149 1105 46-47 1.6243586603373643 1105 48-49 1.6272040906339598 1105 50-51 1.3562694150314627 1105 52-53 1.4567103328139623 1105 54-55 1.5407962174461929 1105 56-57 1.493532146590148 1105 58-59 1.6358248722675555 1105 60-61 1.4809283653346341 1105 62-63 1.4451123542321227 1105 64-65 1.7023912040692508 1105 66-67 1.6549119189070574 1105 68-69 1.631602365908165 1105 70-71 1.2142418465998368 1105 72-73 1.256371928611049 1105 74-75 1.2462795774080035 1105 76-77 1.3850365675809044 1105 78-79 1.2607685876089043 1105 80-81 1.36635298794263 1105 82-83 1.4522758310545107 1105 84-85 1.421080455016952 1105 86-87 1.3664443106826916 1105 88-89 1.2713032839912835 1105 90-91 1.3269340603618573 1105 92-93 1.5664382496047153 1105 94-95 1.6676839882742769 1105 96-97 1.4930086020265918 1105 98-99 1.6006786567002074 1105 100-101 1.7221314426415866 1105 102-103 1.60446110784223 1105 104-105 1.6086775442638839 1105 106-107 1.513221206563685 1105 108-109 1.8259887464365718 1105 110-111 1.6911762785412954 1105 112-113 1.5836379486364116 1105 114-115 1.6316019687677787 1105 116-117 1.78255710945718 1105 118-119 1.5264905671350455 1105 120-121 1.1334849185432638 1106 1 0.3868087656863075 1106 2 0.6005602364728695 1106 3 0.6258394508241771 1106 4 0.761458666751551 1106 5 0.754171344370036 1106 6 0.6505229137873201 1106 7 0.6580881211654415 1106 8 0.7490509000023948 1106 9 0.7280677541501177 1106 10-11 0.78227843057104 1106 12-13 0.8619483660444232 1106 14-15 0.9347623849850564 1106 16-17 0.8996886981953338 1106 18-19 0.6437306008341039 1106 20-21 0.7895459117761661 1106 22-23 0.6873231947241933 1106 24-25 0.8436212234188218 1106 26-27 0.9069893139342611 1106 28-29 0.892514903035746 1106 30-31 0.8422933236771257 1106 32-33 1.0078869287568892 1106 34-35 1.025871858297016 1106 36-37 1.0121916700121432 1106 38-39 0.9684646231635625 1106 40-41 0.8724525888422718 1106 42-43 0.8341875330619004 1106 44-45 0.981263404226496 1106 46-47 0.862308565075601 1106 48-49 1.1510188841644435 1106 50-51 0.9913023165804944 1106 52-53 1.0609216534319117 1106 54-55 0.9695679878653394 1106 56-57 0.7970638211262653 1106 58-59 1.0141111442191786 1106 60-61 1.0400146031162834 1106 62-63 1.0091519448136381 1106 64-65 0.9423621854725042 1106 66-67 1.100340907475644 1106 68-69 1.0202684164797375 1106 70-71 0.8180965827661737 1106 72-73 0.9124264866793048 1106 74-75 0.9901581031286781 1106 76-77 1.0716829652366364 1106 78-79 0.9130683308163299 1106 80-81 0.6144509884810638 1106 82-83 0.6281742167818152 1106 84-85 0.9402240569470415 1106 86-87 0.7554209974371666 1106 88-89 0.7511604690061944 1106 90-91 0.9353499711471471 1106 92-93 0.8535638035725732 1106 94-95 1.0176324744406102 1106 96-97 0.9311136954245356 1106 98-99 1.0375250263986828 1106 100-101 1.0580236415315767 1106 102-103 0.9791375284850439 1106 104-105 1.1736574564573914 1106 106-107 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22441.0 3 3570.0 4 3036.0 5 3600.0 6 4086.0 7 4481.0 8 5359.0 9 6668.0 10 8637.0 11 10719.0 12 11261.0 13 12484.0 14 15312.0 15 15665.0 16 13612.0 17 13956.0 18 15062.0 19 16279.0 20 17698.0 21 19774.0 22 21609.0 23 23904.0 24 26258.0 25 29853.0 26 33679.0 27 38368.0 28 45226.0 29 52142.0 30 61756.0 31 73072.0 32 88462.0 33 107567.0 34 133250.0 35 167755.0 36 212103.0 37 219363.0 38 73857.0 39 417.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.923862021446105 17.4397558859899 13.465336179689565 21.17104591287443 2 13.482155824812194 22.198654540847386 36.357680767103304 27.96150886723711 3 25.524698320483306 23.137961403562983 31.067937763631587 20.26940251232212 4 10.611409324228548 14.692929743571048 44.50212346826353 30.193537463936877 5 10.093133253286483 39.39806663992945 36.4870885831893 14.021711523594767 6 25.950392690542053 35.77470168126432 18.594459565358193 19.68044606283543 7 23.718924623289446 32.7793787272534 24.987580970834436 18.514115678622712 8 21.584389877440472 26.917008208115195 21.62244647055989 29.87615544388444 9 19.689397019012045 21.07986986750753 29.25584033192929 29.974892781551137 10-11 24.798517872388267 22.15991435387959 28.50136586535076 24.540201908381384 12-13 29.694978958527418 21.635125050348798 27.14930673104723 21.520589260076555 14-15 23.50604144265641 23.902379355709183 32.251082585379756 20.34049661625464 16-17 19.137870201644773 28.215746866698677 31.266185556690463 21.38019737496609 18-19 17.5020675383499 32.7533615268508 29.164096408478823 20.580474526320476 20-21 20.510763512371373 26.98651629074231 26.09683190156673 26.405888295319592 22-23 18.160236875341383 26.696856903467857 29.26784659454623 25.87505962664452 24-25 24.036015685964372 25.443507853176705 27.369499362688188 23.150977098170735 26-27 21.812427305038483 22.544435639955083 34.708872608306805 20.93426444669963 28-29 18.223150442434125 30.735274623713927 30.104585451106065 20.936989482745886 30-31 19.43451202813812 26.049825759099786 28.857333831473497 25.65832838128859 32-33 21.158975594984273 25.683729783512867 31.08963543887622 22.067659182626645 34-35 24.86087825862567 23.93580018758682 29.02558381376059 22.177737740026917 36-37 22.09965191557701 23.126349514839177 34.149424711830484 20.62457385775333 38-39 21.3920680613306 27.38139716709854 31.437851234816844 19.788683536754014 40-41 19.87688134855658 29.568349148127478 30.284264511626667 20.270504991689275 42-43 18.36690608129219 28.76569661559134 26.245399527598817 26.621997775517652 44-45 20.086516683815507 25.690140496914754 26.779292849705072 27.444049969564666 46-47 23.97561586019595 27.711623250827277 28.527280760567646 19.78548012840913 48-49 22.408132782801047 25.855345303692424 29.460790023924854 22.27573188958167 50-51 16.753564543811645 27.738457634541806 34.4920129059497 21.01596491569685 52-53 19.938842437390903 29.83173945246801 27.654020989230993 22.575397120910097 54-55 17.936278757566804 26.01256426453191 30.247564468497245 25.803592509404037 56-57 22.70489443808373 23.61462255852415 28.53027103856427 25.150211964827847 58-59 26.532739856815184 24.219083952242055 27.50823005137852 21.73994613956424 60-61 22.9439976753927 25.321619620674785 33.21146662009209 18.522916083840425 62-63 19.58692223255078 27.50631662744012 31.668059373825674 21.23870176618342 64-65 17.977733275778053 32.47718311199579 28.33842503914181 21.20665857308434 66-67 20.146096371692902 27.291964081551935 25.582098868432922 26.97984067832224 68-69 18.331253334025636 27.520430661847744 28.58836968232569 25.559946321800926 70-71 23.9415860323724 25.843368578771887 26.535482180099123 23.679563208756594 72-73 21.68866475945098 23.17925396052212 33.63495199404593 21.497129285980975 74-75 18.31401144322418 30.763733677209093 29.49886516672297 21.423389712843754 76-77 19.436017125987295 26.24399894894663 28.462216176947923 25.85776774811815 78-79 21.1350587111599 26.146609791245428 30.17456374529041 22.543767752304262 80-81 24.403033169138254 24.8808293970758 27.927364330220527 22.78877310356542 82-83 21.276514145155907 23.799597120771292 33.39613952252656 21.527749211546237 84-85 21.01475173954526 27.371658876612088 30.37706997074633 21.236519413096318 86-87 20.182414829597374 29.52089677914736 29.324917991841843 20.971770399413423 88-89 18.67796941284987 28.110923858545867 26.088565242819044 27.122541485785224 90-91 19.791039995845036 24.80287112875871 27.279278421460383 28.126810453935867 92-93 23.268567309442947 26.501902116206672 29.12035278251147 21.109177791838906 94-95 21.48799132576357 24.879455807880994 30.009569354328214 23.622983512027222 96-97 16.161014790580126 26.944586637782876 34.37091194152021 22.523486630116796 98-99 19.288209304511746 28.82859927665659 28.390579466348697 23.492611952482967 100-101 16.897391216162735 25.504930890850087 30.769309254788666 26.828368638198512 102-103 21.447539609850278 23.573737827719988 29.34920128081921 25.629521281610522 104-105 24.587854009761696 24.516551362631603 27.98448093403686 22.911113693569842 106-107 21.64827977986424 24.78350823901902 33.00500269204545 20.563209289071285 108-109 19.218293493670707 26.54857300747857 31.672709504001066 22.560423994849653 110-111 18.357750106705232 30.345514779717448 28.135843407100307 23.160891706477017 112-113 20.167714223716644 26.113195509568964 25.878337484614296 27.840752782100097 114-115 18.3284091253333 25.62946710363217 28.896291171850226 27.145832599184306 116-117 23.20841421506636 24.639425678370735 26.635624984153466 25.51653512240944 118-119 21.46217292755826 22.970333641794287 32.10237523177084 23.465118198876617 120-121 18.968709641322835 29.620865679521884 28.571207775820746 22.839216903334535 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3716.0 1 4318.5 2 3003.5 3 1092.5 4 982.0 5 930.5 6 986.0 7 1081.0 8 1231.5 9 1232.5 10 1147.0 11 1128.0 12 1188.0 13 1261.0 14 1351.0 15 1477.0 16 1631.5 17 1797.5 18 1929.0 19 2083.0 20 2395.0 21 2718.0 22 3065.5 23 3721.5 24 4679.5 25 5867.0 26 7386.0 27 9467.0 28 12154.5 29 15180.0 30 18557.5 31 21747.5 32 24576.5 33 27238.5 34 29489.5 35 31890.5 36 34524.5 37 37220.0 38 39620.5 39 40603.0 40 41439.0 41 42966.5 42 44582.0 43 46651.0 44 47805.0 45 50510.5 46 61372.5 47 108907.5 48 213099.5 49 211001.5 50 106552.0 51 56386.0 52 38649.5 53 30691.0 54 25664.0 55 21799.0 56 18254.0 57 14730.5 58 11765.0 59 9543.5 60 7613.0 61 5848.5 62 4323.0 63 3002.5 64 2006.0 65 1305.0 66 803.0 67 461.0 68 269.0 69 146.0 70 87.5 71 66.5 72 50.0 73 31.5 74 26.0 75 22.0 76 15.0 77 11.5 78 9.5 79 7.5 80 6.5 81 6.0 82 4.0 83 5.0 84 4.5 85 3.0 86 2.0 87 1.5 88 3.0 89 3.0 90 1.5 91 0.5 92 0.0 93 2.0 94 3.0 95 2.5 96 2.0 97 1.5 98 1.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4209292053559887 2 0.8764100148192076 3 0.8770226319133073 4 0.857541408320933 5 0.8651378602877707 6 0.8743883784086781 7 0.8489035072941254 8 0.8392241572073482 9 0.8382439698567884 10-11 0.8323628457534302 12-13 0.8351808843862893 14-15 0.8360079174633241 16-17 0.8576639317397529 18-19 0.8867632437094946 20-21 0.8791974225973618 22-23 0.8538044440469241 24-25 0.846881870883596 26-27 0.8483827827641406 28-29 0.8693342873823546 30-31 0.8774208330244723 32-33 0.8718153866134589 34-35 0.8836082656748805 36-37 0.8962588086680419 38-39 0.881464105845531 40-41 0.8914803953340631 42-43 0.8844046678972102 44-45 0.8672207584077102 46-47 0.8623198216549116 48-49 0.8668225572965452 50-51 0.8967182714886166 52-53 0.871478447211704 54-55 0.8833325879825354 56-57 0.9317599692711266 58-59 0.8969633183262566 60-61 0.9105634178152726 62-63 0.881494736700236 64-65 0.8690892405447147 66-67 0.8718153866134589 68-69 0.8914803953340631 70-71 0.8909290399493733 72-73 0.8959525001209919 74-75 0.9007002826002655 76-77 0.9137796575592968 78-79 0.9694971822676757 80-81 0.9970649515021678 82-83 0.9634935347454974 84-85 0.9255419057660133 86-87 0.9078372717465285 88-89 0.9066120375583289 90-91 0.9188031177309153 92-93 0.9067039301224439 94-95 0.899138109010311 96-97 0.9082048420029883 98-99 0.9437366334607782 100-101 0.9305041042282219 102-103 0.9056012193530641 104-105 0.8937470785822325 106-107 0.8951560978986621 108-109 0.8935326625992976 110-111 0.8923686901205079 112-113 0.9047129245666193 114-115 0.9080210568747584 116-117 0.9353437792716105 118-119 0.9294932860229572 120-121 0.9075309631994785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1632341.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.99801958552129 #Duplication Level Percentage of deduplicated Percentage of total 1 85.35361608017764 42.67511768471787 2 9.401258308547535 9.400885940786088 3 2.162865676616099 3.244170013809104 4 0.8573745548512239 1.7146811914231639 5 0.49991232113429823 1.2497313011558024 6 0.3034084705387549 0.9101893591445837 7 0.2259304926451414 0.7907254037366779 8 0.16077510903908923 0.6430749640480563 9 0.1420690913466813 0.6392855890483726 >10 0.7879985806223587 7.335715032465327 >50 0.06437028016271581 2.1947111551440384 >100 0.03150834583152643 3.0297605740513562 >500 0.0026361472989651297 0.9403141550774059 >1k 0.0038914555365675723 3.845358940947971 >5k 0.001380839061362687 5.27641641324272 >10k+ 0.001004246590081954 16.109862281201334 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 68819 4.215969579885575 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 53388 3.2706401419801376 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 42213 2.586040539323585 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 32668 2.0012975230053036 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 20176 1.236016249055804 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 14755 0.9039165223442897 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 13759 0.8428998597719471 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 10900 0.6677526325688076 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9505 0.5822925479418822 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 9232 0.5655681012729571 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9115 0.5584004812719892 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8349 0.5114740118639427 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 8139 0.4986090528878464 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7668 0.46975478775574464 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7108 0.43544823048615455 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7008 0.42932205954515634 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 6551 0.4013254583447944 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6355 0.38931816330043784 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5039 0.30869775371690106 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4814 0.29491386909965506 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4087 0.2503766063585979 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3884 0.23794047934837143 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3117 0.190952748230915 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3114 0.19076896310268504 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3023 0.18519414754637664 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2911 0.17833283609245862 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2907 0.1780877892548187 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2745 0.16816339233040156 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2655 0.16264983848350315 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2153 0.131896460359692 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2022 0.12387117642698431 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1785 0.1093521512968185 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1670 0.10230705471467051 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.225234188199647E-4 0.0 9 0.0 0.0 0.0 1.225234188199647E-4 0.0 10-11 0.0 0.0 0.0 2.1441598293493822E-4 0.0 12-13 0.0 0.0 0.0 2.450468376399294E-4 0.0 14-15 0.0 0.0 0.0 3.982011111648853E-4 0.0 16-17 0.0 0.0 0.0 5.513553846898411E-4 0.0 18-19 0.0 0.0 0.0 6.738788035098059E-4 0.0 20-21 0.0 0.0 0.0 0.0011027107693796822 0.0 22-23 0.0 0.0 0.0 0.001960374701119435 0.0 24-25 0.0 0.0 0.0 0.0032162397440240733 0.0 26-27 0.0 0.0 0.0 0.00496219846220857 0.0 28-29 0.0 0.0 0.0 0.009893766069712151 0.0 30-31 0.0 0.0 0.0 0.02058393436175407 0.0 32-33 0.0 0.0 0.0 0.033724571030195286 0.0 34-35 0.0 0.0 0.0 0.046650791715701564 0.0 36-37 0.0 3.063085470499117E-5 0.0 0.06156801795703226 0.0 38-39 0.0 6.126170940998235E-5 0.0 0.08754298274686478 0.0 40-41 0.0 6.126170940998235E-5 0.0 0.11756122035775612 0.0 42-43 0.0 6.126170940998235E-5 0.0 0.14322987660053874 0.0 44-45 0.0 6.126170940998235E-5 0.0 0.15324616608907085 0.0 46-47 0.0 6.126170940998235E-5 0.0 0.16886790198861634 0.0 48-49 0.0 6.126170940998235E-5 0.0 0.1898500374615353 0.0 50-51 0.0 1.225234188199647E-4 0.0 0.21058712609681435 0.0 52-53 6.126170940998235E-5 1.225234188199647E-4 0.0 0.2594433393512753 0.0 54-55 6.126170940998235E-5 1.225234188199647E-4 0.0 0.2911769048256461 0.0 56-57 6.126170940998235E-5 1.225234188199647E-4 0.0 0.3771883448372614 0.0 58-59 6.126170940998235E-5 1.225234188199647E-4 0.0 0.5350291391320808 0.0 60-61 6.126170940998235E-5 1.225234188199647E-4 0.0 0.6396641388043307 0.0 62-63 6.126170940998235E-5 1.225234188199647E-4 0.0 0.6953510326580047 0.0 64-65 6.126170940998235E-5 1.225234188199647E-4 0.0 0.8244600852395425 0.0 66-67 6.126170940998235E-5 1.225234188199647E-4 0.0 1.1728860575088171 0.0 68-69 6.126170940998235E-5 1.225234188199647E-4 0.0 1.5424473195245356 0.0 70-71 6.126170940998235E-5 1.225234188199647E-4 0.0 1.7714435892990497 0.0 72-73 6.126170940998235E-5 1.225234188199647E-4 0.0 2.009414699502126 0.0 74-75 6.126170940998235E-5 1.225234188199647E-4 0.0 2.3024600864647766 0.0 76-77 6.126170940998235E-5 1.225234188199647E-4 0.0 2.409545554513426 0.0 78-79 6.126170940998235E-5 1.225234188199647E-4 0.0 2.6016010135137204 0.0 80-81 6.126170940998235E-5 1.225234188199647E-4 0.0 2.8699273007294432 0.0 82-83 6.126170940998235E-5 1.225234188199647E-4 0.0 3.0706512916112505 0.0 84-85 6.126170940998235E-5 1.225234188199647E-4 0.0 3.4869246070520803 0.0 86-87 6.126170940998235E-5 1.225234188199647E-4 0.0 4.3428119492189445 0.0 88-89 6.126170940998235E-5 1.5315427352495588E-4 0.0 5.422580208424588 0.0 90-91 6.126170940998235E-5 1.8378512822994706E-4 0.0 6.598069888583329 0.0 92-93 6.126170940998235E-5 1.8378512822994706E-4 0.0 7.700811288817716 0.0 94-95 6.126170940998235E-5 1.8378512822994706E-4 0.0 8.54165888132443 0.0 96-97 6.126170940998235E-5 1.8378512822994706E-4 0.0 9.399537229047116 0.0 98-99 6.126170940998235E-5 1.8378512822994706E-4 0.0 10.358742444133917 0.0 100-101 6.126170940998235E-5 1.8378512822994706E-4 0.0 11.206175670402201 0.0 102-103 6.126170940998235E-5 3.0630854704991175E-4 0.0 11.858306567071464 0.0 104-105 6.126170940998235E-5 3.0630854704991175E-4 0.0 12.424579178002634 0.0 106-107 6.126170940998235E-5 3.0630854704991175E-4 0.0 13.315201909404959 0.0 108-109 6.126170940998235E-5 3.0630854704991175E-4 0.0 14.228675258417205 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1600 0.0 61.477703 2 TATAACG 190 0.0 51.46795 2 ACCTGGG 1930 0.0 50.599052 3 GTACCTG 2460 0.0 40.763927 1 CCTGGGG 1300 0.0 37.561226 4 GTACCGG 115 8.548377E-7 35.0802 1 GTATAAC 420 0.0 34.304615 1 TAACGCA 340 0.0 32.102505 4 ATAACGC 335 0.0 30.865868 3 GAGTACG 1205 0.0 30.131126 1 GTACCCG 135 2.9895837E-6 29.883133 1 AGTACGC 1160 0.0 29.753277 2 TCACGCA 265 1.8189894E-12 28.181343 4 GTACGCA 1190 0.0 27.998312 3 TATCACG 260 5.638867E-11 26.549078 2 TACGCAG 1310 0.0 25.434366 4 ACACGGG 165 1.4578482E-5 24.370525 3 ACGCAGT 1390 0.0 23.971998 5 CTGGGGG 930 0.0 23.474249 5 CGCAGTG 1485 0.0 23.212173 6 >>END_MODULE