##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003804_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1632341 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.613914617105127 31.0 31.0 34.0 30.0 34.0 2 31.79195707269498 31.0 31.0 34.0 30.0 34.0 3 31.869699407170437 31.0 31.0 34.0 30.0 34.0 4 35.486891525729 37.0 35.0 37.0 33.0 37.0 5 35.41983997216268 37.0 35.0 37.0 33.0 37.0 6 35.54210731703731 37.0 35.0 37.0 33.0 37.0 7 35.497961516619384 37.0 35.0 37.0 33.0 37.0 8 35.52278169818684 37.0 35.0 37.0 33.0 37.0 9 37.05944529972598 39.0 37.0 39.0 33.0 39.0 10-11 36.95418328645792 39.0 37.0 39.0 33.0 39.0 12-13 36.93311354674054 39.0 37.0 39.0 33.0 39.0 14-15 37.82543720950463 39.0 37.0 41.0 32.5 41.0 16-17 37.99799337270828 39.5 37.0 41.0 33.0 41.0 18-19 38.0855562655107 40.0 37.0 41.0 33.0 41.0 20-21 37.99958342037601 40.0 37.0 41.0 32.5 41.0 22-23 37.86878323830621 39.5 37.0 41.0 32.5 41.0 24-25 37.66773578559872 39.5 36.5 41.0 32.0 41.0 26-27 37.34401421026611 39.0 36.0 40.5 31.5 41.0 28-29 37.2919558474608 39.0 36.0 40.0 31.5 41.0 30-31 37.17946893449347 39.0 36.0 40.0 31.0 41.0 32-33 36.90871668358511 39.0 35.5 40.0 31.0 41.0 34-35 36.75370281087101 39.0 35.0 40.0 30.0 41.0 36-37 36.52919549285352 38.5 35.0 40.0 30.0 41.0 38-39 36.315052430834 38.0 35.0 40.0 30.0 41.0 40-41 36.66658620962164 39.0 35.0 40.0 30.0 41.0 42-43 36.53973985827716 39.0 35.0 40.5 30.0 41.0 44-45 36.363555164025165 39.0 35.0 40.5 30.0 41.0 46-47 36.09437029395206 39.0 35.0 40.0 29.5 41.0 48-49 35.770744286886135 38.5 35.0 40.0 28.0 41.0 50-51 35.4213068837945 38.0 34.0 40.0 27.5 41.0 52-53 35.43928137564394 38.0 34.0 40.0 27.5 41.0 54-55 35.270532015063026 38.0 34.0 40.0 27.0 41.0 56-57 34.84682367225966 38.0 33.5 40.0 25.5 41.0 58-59 34.56106046469457 38.0 34.0 40.0 26.0 41.0 60-61 33.84275558844629 37.0 32.0 40.0 22.5 41.0 62-63 33.81580441831701 37.0 32.5 40.0 23.5 41.0 64-65 33.41012631551863 36.5 32.0 39.0 22.5 40.5 66-67 32.94052253787658 36.0 31.5 39.0 22.0 40.0 68-69 32.476863290207135 35.5 31.0 38.0 21.5 40.0 70-71 31.843155321100188 35.0 31.0 37.5 20.5 40.0 72-73 31.365234960097187 34.5 30.5 37.0 19.0 39.0 74-75 31.094448096323013 34.0 30.0 36.5 18.5 39.0 76-77 30.220906967355475 34.0 29.5 35.0 18.0 38.0 78-79 30.59730840553536 34.0 30.0 36.0 18.0 37.5 80-81 30.211341870356748 34.0 29.5 35.0 13.5 37.0 82-83 29.870462728069686 34.0 29.5 35.0 10.5 37.0 84-85 29.548677022754436 34.0 29.0 35.0 7.0 36.0 86-87 29.450798577013014 34.0 29.0 35.0 7.0 36.0 88-89 29.119790533963183 34.0 29.0 35.0 2.0 36.0 90-91 28.675022253315944 34.0 29.0 35.0 2.0 35.0 92-93 28.372719915752896 34.0 29.0 35.0 2.0 35.0 94-95 27.946220183160257 33.0 27.5 35.0 2.0 35.0 96-97 27.445100625420793 33.0 26.5 35.0 2.0 35.0 98-99 27.260420157307816 34.0 27.0 35.0 2.0 35.0 100-101 26.144875672423836 33.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.08452528631821465 1101 2 0.007878182105823583 1101 3 0.03379732403582736 1101 4 0.07754785793731855 1101 5 -0.0743061641468401 1101 6 0.016906704251447024 1101 7 -0.049975572112096245 1101 8 -0.0468918277072774 1101 9 0.07990170043797917 1101 10-11 0.05766363520043427 1101 12-13 0.050952878367588994 1101 14-15 -0.015246078253966289 1101 16-17 0.01628994707134268 1101 18-19 -0.04781459990535808 1101 20-21 -0.16267952171791222 1101 22-23 -0.036417471289041714 1101 24-25 -0.0949785231439293 1101 26-27 -0.026657459390285965 1101 28-29 0.022448532481575967 1101 30-31 0.14843930805477612 1101 32-33 0.166150658288565 1101 34-35 0.0966176808730097 1101 36-37 -0.061473767956513825 1101 38-39 0.023244035011302344 1101 40-41 0.05013931279693651 1101 42-43 -0.08632636181988573 1101 44-45 0.014182294075574475 1101 46-47 0.14991112258385897 1101 48-49 0.16603040674075942 1101 50-51 0.2926840172185834 1101 52-53 0.291937892572669 1101 54-55 0.27113421123701187 1101 56-57 0.19338020382003407 1101 58-59 -0.08167695196268454 1101 60-61 -0.008970286703679164 1101 62-63 -0.07776906663186622 1101 64-65 -0.12796540251177646 1101 66-67 -0.1392280674374291 1101 68-69 -0.10631252793056234 1101 70-71 -0.03332192763014419 1101 72-73 0.1269216178385726 1101 74-75 -0.21435491671772766 1101 76-77 -0.31525499539196744 1101 78-79 -0.0477172442735494 1101 80-81 -0.03674185186661916 1101 82-83 -0.17519439183829633 1101 84-85 -0.11646024371031061 1101 86-87 -0.07290217594039561 1101 88-89 0.06850750278480433 1101 90-91 -0.012595564327419595 1101 92-93 0.04419903124223268 1101 94-95 0.01170673835779823 1101 96-97 0.13948845323190895 1101 98-99 -0.2286423528314181 1101 100-101 -0.061333964539979746 1102 1 -0.0290194990237147 1102 2 -0.05386912875764338 1102 3 -0.131886208187165 1102 4 -0.05106024929805386 1102 5 -0.10185612575623537 1102 6 0.031812019377341016 1102 7 -0.18874721616298729 1102 8 -0.1590902820368072 1102 9 -0.10310080392132903 1102 10-11 -0.15689092610614352 1102 12-13 -0.1640663886947138 1102 14-15 -0.038430130723519085 1102 16-17 -0.018547341331824896 1102 18-19 -0.1625181311237398 1102 20-21 -0.15542950916023557 1102 22-23 -0.05111597943717783 1102 24-25 -0.13651816593187505 1102 26-27 -0.20205187804166513 1102 28-29 -0.1447935866800023 1102 30-31 -0.2930394838250763 1102 32-33 -0.21461198729076614 1102 34-35 -0.40178598740342863 1102 36-37 -0.47058733957472754 1102 38-39 -0.31410981158413165 1102 40-41 -0.1800192009483368 1102 42-43 -0.2009862640852873 1102 44-45 -0.12109042275960036 1102 46-47 -0.23119639264681524 1102 48-49 -0.34243231849012545 1102 50-51 -0.1267699032877445 1102 52-53 -0.07387652650925958 1102 54-55 -0.060134831581066805 1102 56-57 -0.10534092249583438 1102 58-59 -0.16442012238110948 1102 60-61 -0.12368529922547822 1102 62-63 -0.27038013030286123 1102 64-65 -0.32921528590478744 1102 66-67 -0.3045119067852937 1102 68-69 -0.2761310515784601 1102 70-71 -0.31971270814350206 1102 72-73 -0.2997119922420133 1102 74-75 -0.26845941093043635 1102 76-77 -0.29607504748837954 1102 78-79 -0.3719320307571863 1102 80-81 -0.21356569279316417 1102 82-83 -0.2913530347482336 1102 84-85 -0.39233013183936194 1102 86-87 -0.4956236753986225 1102 88-89 -0.5196643149207993 1102 90-91 -0.22387206999272635 1102 92-93 -0.3333870247147708 1102 94-95 -0.3098036271064437 1102 96-97 -0.26301231816816184 1102 98-99 -0.6115234424071154 1102 100-101 -0.5057908630093806 1103 1 -0.18152222205378976 1103 2 -0.19013003081601454 1103 3 -0.09316336892146992 1103 4 0.003548866691097885 1103 5 -0.09207579676650113 1103 6 -0.03979389257933974 1103 7 -0.0816895992317086 1103 8 -0.1068631217000302 1103 9 -0.26021796974391975 1103 10-11 -0.1721441877793879 1103 12-13 -0.18471228347841162 1103 14-15 -0.13199169505461583 1103 16-17 -0.27238510439243413 1103 18-19 -0.15455750176590044 1103 20-21 -0.11816955320573896 1103 22-23 -0.07509867925192992 1103 24-25 -0.12976441530577887 1103 26-27 -0.18037353746016294 1103 28-29 -0.2981187045454661 1103 30-31 0.0021967638792332878 1103 32-33 0.038750497702757514 1103 34-35 -0.1316464569571707 1103 36-37 -0.15664001927483895 1103 38-39 -0.29991714031149996 1103 40-41 -0.24549197462279437 1103 42-43 -0.06134435247841452 1103 44-45 -0.02640530044187983 1103 46-47 0.1530966928444002 1103 48-49 0.2292806065609625 1103 50-51 0.19876122671011132 1103 52-53 -0.041863233352216866 1103 54-55 -0.10010557391484554 1103 56-57 0.2000070290700151 1103 58-59 -0.06792166079372208 1103 60-61 0.12001977077825643 1103 62-63 -0.09221525381314422 1103 64-65 0.07503550317765217 1103 66-67 -0.05467592462611037 1103 68-69 -0.12457316035656163 1103 70-71 -0.08823273135882559 1103 72-73 0.042629416101188866 1103 74-75 0.08968447636394572 1103 76-77 0.17303807950420236 1103 78-79 0.07223750469785628 1103 80-81 0.17556190402347838 1103 82-83 0.10812891374873601 1103 84-85 0.07919770667103876 1103 86-87 -0.053022764276732914 1103 88-89 -0.015578418228983537 1103 90-91 -0.04797688876215389 1103 92-93 -0.05717859564473571 1103 94-95 -0.1817867085429299 1103 96-97 0.0946753721458009 1103 98-99 0.1361492215510971 1103 100-101 0.3054412186576734 1104 1 -0.059580276669603194 1104 2 -0.03973708294583034 1104 3 -0.006850758570536186 1104 4 -0.005141927781259881 1104 5 -0.02141789791625115 1104 6 -0.1420187848958605 1104 7 0.03369993022307227 1104 8 -0.22048805560839924 1104 9 -0.18497851076422478 1104 10-11 -0.13597677513248385 1104 12-13 -0.18508929460083579 1104 14-15 -0.1352763980874201 1104 16-17 -0.015372595574554282 1104 18-19 -0.033593168819031405 1104 20-21 -0.22446868018457877 1104 22-23 -0.18926457909711303 1104 24-25 -0.15901038626487463 1104 26-27 -0.1507238808647955 1104 28-29 -0.20330694845857522 1104 30-31 -0.1665242934237412 1104 32-33 -0.24302156670753305 1104 34-35 -0.13634711794142618 1104 36-37 -0.3018000051934209 1104 38-39 -0.1563193974171213 1104 40-41 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2 0.11754818003231193 1105 3 0.1688115657854361 1105 4 0.15928649727728583 1105 5 0.10804791248577317 1105 6 0.12052634586136435 1105 7 0.09074764685070846 1105 8 0.14503143599971935 1105 9 0.2081927177681706 1105 10-11 0.1856347588253655 1105 12-13 0.13256567466145697 1105 14-15 0.19925305278103878 1105 16-17 0.18472284987822007 1105 18-19 0.14793920335115018 1105 20-21 0.1170728096183069 1105 22-23 0.19763865811990655 1105 24-25 0.21437420707041355 1105 26-27 0.32366212738071454 1105 28-29 0.20253214819442178 1105 30-31 0.2225600096109943 1105 32-33 0.231594669387313 1105 34-35 0.18986516128378383 1105 36-37 0.3180770439869107 1105 38-39 0.4914693479478274 1105 40-41 0.32581163523887824 1105 42-43 0.19134785406539834 1105 44-45 0.2615508443636898 1105 46-47 -0.05212426079749832 1105 48-49 -0.03296581592162795 1105 50-51 0.09899315321910507 1105 52-53 0.16751902143594322 1105 54-55 0.19151631710614936 1105 56-57 0.08812119223019721 1105 58-59 0.07348530712803125 1105 60-61 -0.05078325870037048 1105 62-63 -0.12078948389875421 1105 64-65 -0.014826194865982245 1105 66-67 0.28760460291790224 1105 68-69 0.35714609596720237 1105 70-71 0.28893312518983194 1105 72-73 0.36549223863697833 1105 74-75 -0.020966724001208803 1105 76-77 -0.15997558972582482 1105 78-79 0.036506539448293296 1105 80-81 0.07310275754930373 1105 82-83 0.14801607912163206 1105 84-85 0.1606236352839261 1105 86-87 0.20367355633653617 1105 88-89 0.12537474757920108 1105 90-91 0.018114231377136747 1105 92-93 0.24440389280805874 1105 94-95 0.25820240171775666 1105 96-97 0.20530692412635787 1105 98-99 0.12166666175955143 1105 100-101 0.2400640528011202 1106 1 -0.06651253169526328 1106 2 -0.011493973297710625 1106 3 0.04729586830366017 1106 4 0.08777287070339668 1106 5 0.07082722618689985 1106 6 0.06595274579509436 1106 7 -0.014216319352073015 1106 8 0.006842341400648877 1106 9 0.02619356684040497 1106 10-11 -0.03971108279160518 1106 12-13 -0.14171701461952324 1106 14-15 0.03473689452226125 1106 16-17 -0.03449439871356219 1106 18-19 0.0406894725691771 1106 20-21 -0.0133807968677786 1106 22-23 0.0021928434247442397 1106 24-25 0.03967435928213803 1106 26-27 -0.01963550867792918 1106 28-29 0.12367650600589286 1106 30-31 -0.08881587570211735 1106 32-33 0.15533672227829953 1106 34-35 0.24574925774668088 1106 36-37 0.2216141546562298 1106 38-39 0.3572376288871908 1106 40-41 0.10629669363848393 1106 42-43 0.30389004881357806 1106 44-45 0.36519460678913873 1106 46-47 0.4520783878936925 1106 48-49 0.39376093296008463 1106 50-51 0.5607473675166545 1106 52-53 0.6292589982417738 1106 54-55 0.5234110166174091 1106 56-57 0.6022253692513999 1106 58-59 0.5027489181701057 1106 60-61 0.5637366530789549 1106 62-63 0.233284639071762 1106 64-65 0.2333869999236171 1106 66-67 0.14063874183439395 1106 68-69 0.30517472109145416 1106 70-71 0.30369352114741943 1106 72-73 0.2923691283404253 1106 74-75 0.4137472577333803 1106 76-77 0.25365978552268587 1106 78-79 0.4191261861514093 1106 80-81 0.31152249951417943 1106 82-83 0.4266405562173894 1106 84-85 0.4209264020168604 1106 86-87 0.494923452791145 1106 88-89 0.42653846837939824 1106 90-91 0.3934455766389 1106 92-93 0.3816992612225718 1106 94-95 0.4502771666697143 1106 96-97 0.4610388088596231 1106 98-99 0.3214917994749307 1106 100-101 0.47977228776633396 1107 1 -0.17530219318101814 1107 2 -0.0998771032839798 1107 3 -0.08502811553698919 1107 4 -0.1776841485986509 1107 5 -0.12074996370713365 1107 6 -0.05050190055990811 1107 7 -0.13427578532379414 1107 8 -0.06992947487280787 1107 9 0.031820337911319996 1107 10-11 -0.12074092283788929 1107 12-13 -0.14123795306519327 1107 14-15 -0.1597458341906659 1107 16-17 -0.20536826932764285 1107 18-19 -0.18132811515601333 1107 20-21 -0.13121570968128538 1107 22-23 -0.20369724210164009 1107 24-25 -0.1309115323212069 1107 26-27 -0.09274558713018877 1107 28-29 -0.20819592674592968 1107 30-31 -0.2192276789115013 1107 32-33 -0.0997076535932635 1107 34-35 -0.11078095430073631 1107 36-37 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2316 64-65 -0.8074626774868747 2316 66-67 -0.7249608385760524 2316 68-69 -0.64615465155007 2316 70-71 -0.8044795475217406 2316 72-73 -0.8104998409182862 2316 74-75 -0.844893685896718 2316 76-77 -0.8520430012111433 2316 78-79 -0.7395317539454886 2316 80-81 -0.8416377703004372 2316 82-83 -0.9177475623939131 2316 84-85 -0.9864780486538969 2316 86-87 -0.9775049302783145 2316 88-89 -0.8398586154389349 2316 90-91 -1.085836545824936 2316 92-93 -0.9758016744415059 2316 94-95 -1.067988026283107 2316 96-97 -1.0292856519746785 2316 98-99 -0.986704789557372 2316 100-101 -1.2675342443707258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 7.0 5 28.0 6 83.0 7 236.0 8 538.0 9 992.0 10 1720.0 11 2943.0 12 4760.0 13 6800.0 14 7513.0 15 9135.0 16 10805.0 17 13746.0 18 12875.0 19 10520.0 20 10139.0 21 10372.0 22 11184.0 23 12013.0 24 13943.0 25 17010.0 26 20704.0 27 25902.0 28 32598.0 29 40602.0 30 50152.0 31 61385.0 32 76081.0 33 94697.0 34 121624.0 35 163322.0 36 230927.0 37 284878.0 38 228251.0 39 43826.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.02330199433692 19.30345300989201 13.20215491352153 18.471090082249546 2 14.2806468312437 27.894245113385384 35.20255595751915 22.62255209785176 3 29.936793306819986 24.76564860559771 27.300596764832246 17.996961322750057 4 12.380195069535104 16.485219693679202 42.185119408260896 28.9494658285248 5 11.301743937081774 41.91740573813927 33.0188973995017 13.761952925277255 6 26.539697865059296 41.33020730654646 16.22889426502328 15.901200563370962 7 28.102951527897662 33.71623943771553 21.13798526165795 17.04282377272886 8 24.111689898127903 28.368949870155806 18.938567370420763 28.580792861295524 9 21.440005489049163 25.58246101764276 23.658475771912855 29.319057721395225 10-11 25.94096799982847 24.652261943779923 27.915685199422914 21.491084856968698 12-13 32.757309900321076 22.97963476994084 24.413158770134427 19.84989655960366 14-15 22.52795590034448 27.626641622296837 30.44538912199512 19.40001335536356 16-17 19.380999435779657 28.62618778796832 30.040965705082456 21.951847071169567 18-19 18.797175345102524 35.15350040218313 27.192234955808864 18.85708929690549 20-21 23.41219549778314 26.534852922660672 25.904549914637215 24.14840166491897 22-23 20.089736151943743 28.87748331996807 26.52766793212938 24.50511259595881 24-25 25.272476768028252 28.624074258993677 26.689000643860567 19.414448329117505 26-27 24.697896857294097 23.94058658615484 32.353800703824966 19.007715852726097 28-29 19.282832522048665 34.405750573617574 28.053008696930803 18.258408207402958 30-31 22.718645097927492 27.18112067502483 26.19758763908571 23.902646587961968 32-33 22.64214289630903 29.006864495069507 27.75965169327173 20.59134091534974 34-35 25.91512147603104 25.551597062886938 29.571594428430075 18.961687032651948 36-37 24.49860999881741 24.858043410193424 31.20505167544621 19.438294915542954 38-39 20.342695990719132 29.520508058110174 31.83701752660789 18.2997784245628 40-41 21.32253603710963 30.17807328674002 27.07505558663274 21.42433508951761 42-43 19.325680112182443 31.70939773000862 24.955845622942753 24.009076534866182 44-45 23.68239846943745 24.90230288891843 26.148794890283337 25.266503751360776 46-47 26.180138273846666 30.453113183186964 25.535327953940985 17.83142058902539 48-49 24.8689765189994 28.966864153997236 27.262685921630347 18.90147340537302 50-51 18.316883543328263 29.48259585466517 32.309946267354675 19.890574334651888 52-53 22.71654635887967 33.07198067070545 24.794666065485092 19.41680690492979 54-55 19.94261615679567 29.05642877315463 26.2798949484207 24.721060121628998 56-57 23.909823860752493 26.000043496466525 28.080614268127533 22.009518374653446 58-59 29.71647398790813 25.63539269158268 24.556142512238587 20.091990808270594 60-61 22.24633106060815 29.082856287735158 31.19202735725163 17.478785294405064 62-63 20.179032599005577 28.045928417595682 30.233471498463643 21.541567484935097 64-65 19.59154368063602 35.083876467796046 26.03889117525115 19.285688676316784 66-67 23.116023907243623 27.095836085623915 25.01305167851939 24.77508832861308 68-69 20.610170031467568 29.756165398172108 25.28429706144431 24.349367508916014 70-71 25.4120397356795 29.152659393452268 25.26547069185796 20.16983017901027 72-73 24.845697069423604 24.727125030860584 30.702010180470868 19.725167719244936 74-75 19.668684423168934 34.61543880843524 26.763311097374874 18.952565671020945 76-77 22.728890593325783 27.691517887500222 25.113441370399936 24.46615014877406 78-79 22.60658159048875 29.713521868286097 26.400212945701906 21.279683595523238 80-81 25.557741917895832 26.9598386611621 27.644958988348634 19.837460432593435 82-83 23.766939628423227 25.902430925891096 29.76881056102861 20.561818884657065 84-85 19.95218520651335 29.833643726184494 30.07801683472806 20.136154232574096 86-87 21.478086432705084 30.23352764615809 25.60120220530915 22.68718371582768 88-89 19.66902136257069 31.519945893658246 24.19273913967731 24.61829360409375 90-91 23.426630832650776 24.41294435415149 25.731296340654314 26.42912847254342 92-93 25.13684334339455 29.724457083415786 25.434422096853538 19.70427747633613 94-95 23.836371049037286 28.570557779187478 27.113967406902795 20.47910376487244 96-97 17.65182282963981 29.3148142986274 31.763111634014635 21.270251237718156 98-99 21.69166307518465 32.810401299488554 24.65223886403185 20.845696761294942 100-101 18.793210815327647 29.787791266663667 25.519019880459332 25.89997803754936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 244.0 1 189.5 2 340.0 3 488.5 4 363.0 5 309.5 6 370.0 7 587.5 8 858.5 9 1018.5 10 1072.0 11 1094.5 12 1145.5 13 1271.0 14 1481.5 15 1636.5 16 1745.5 17 1953.0 18 2170.0 19 2307.5 20 2496.0 21 2865.0 22 3361.0 23 3988.0 24 4782.0 25 5801.0 26 7217.5 27 9238.0 28 12075.0 29 15362.0 30 18584.5 31 21464.0 32 24153.5 33 26939.5 34 29383.5 35 31642.0 36 34054.5 37 36237.5 38 38542.0 39 39830.5 40 40550.5 41 41782.0 42 43470.5 43 45617.0 44 46305.0 45 47846.0 46 55767.5 47 111374.0 48 228002.5 49 223771.0 50 111244.5 51 56629.5 52 37786.5 53 29655.0 54 24859.0 55 21266.0 56 17838.5 57 14408.0 58 11655.0 59 9422.5 60 7408.0 61 5667.0 62 4187.5 63 2859.0 64 1823.5 65 1123.5 66 655.5 67 359.0 68 207.0 69 105.5 70 51.0 71 34.0 72 21.0 73 15.0 74 11.5 75 8.5 76 5.0 77 2.5 78 2.0 79 1.0 80 0.5 81 0.0 82 0.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044169692484597275 2 0.004655889915158659 3 0.043495813681087465 4 0.0 5 0.0 6 0.0014702810258395765 7 0.0 8 0.0 9 0.0 10-11 3.675702564598941E-4 12-13 0.0 14-15 0.0023279449575793294 16-17 0.0 18-19 0.0 20-21 0.011609093933191655 22-23 0.0 24-25 0.0 26-27 0.0071676200009679345 28-29 0.009556826667957248 30-31 0.010046920343237105 32-33 0.0062180635051132084 34-35 0.007718975385657776 36-37 0.02009384068647421 38-39 0.01013881290735208 40-41 0.012497388719636399 42-43 0.0 44-45 0.0 46-47 3.063085470499117E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0015009118805445676 58-59 2.450468376399294E-4 60-61 0.009771242650892185 62-63 0.014457763420755837 64-65 0.0419949018005429 66-67 0.02248304735346352 68-69 0.0015621735899545499 70-71 2.756776923449206E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 6.126170940998235E-5 86-87 4.594628205748676E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 9.189256411497353E-5 96-97 1.5315427352495588E-4 98-99 3.982011111648853E-4 100-101 5.819862393948323E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1632341.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.29473912811925 #Duplication Level Percentage of deduplicated Percentage of total 1 83.52930949038218 38.669675924091784 2 10.511234677306005 9.732297346006446 3 2.468594769076277 3.42848852542278 4 0.9604126038789086 1.7784820380772721 5 0.5700795132774699 1.3195841174732832 6 0.36471369845929313 1.0130595315974713 7 0.250685169565335 0.8123783169820866 8 0.18348939860045108 0.6795675072786697 9 0.13609352710328596 0.5670372900845025 >10 0.9031510016866039 7.9326205848906675 >50 0.07526442087790512 2.4327838616500013 >100 0.0375241728435953 3.3084470788683955 >500 0.0029274120104218395 0.9282146576674348 >1k 0.00372579710417325 4.04261261676569 >5k 0.001197577640627116 3.6600659600020338 >10k+ 0.0015967701875028216 19.694684643141468 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 107828 6.605727602259577 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 28037 1.717594546727675 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 25206 1.5441626473880152 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 23683 1.450861063956612 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 21660 1.3269286258202178 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 20728 1.269832712650114 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 19240 1.1786752890480603 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18234 1.1170460093816181 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 17470 1.0702420633923915 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 14985 0.9180067155085856 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 12153 0.7445135544595155 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 10486 0.6423902848730749 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9905 0.6067972317058752 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8664 0.530771450328087 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 7704 0.47196020929450405 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5994 0.3672026862034342 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5886 0.3605864215871561 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5840 0.3577683829542969 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 5321 0.32597355577051607 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5071 0.31065812841802054 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5030 0.3081463983322112 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4833 0.2960778415784447 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 4461 0.27328848567793124 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4304 0.263670397300564 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3829 0.23457108533082244 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3727 0.2283223909710042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3395 0.20798350344689007 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2717 0.16644806446692204 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2585 0.15836151882480437 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2580 0.15805521027775446 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2452 0.15021371147327672 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2437 0.149294785832127 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2422 0.14837586019097726 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2315 0.14182085728410915 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2293 0.14047309967708954 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2250 0.13783884617246028 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2052 0.12570902770928377 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 1979 0.12123692292235508 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1975 0.12099187608471515 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 1864 0.1141918263402071 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1699 0.10408364428756002 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.189256411497352E-5 0.0 12-13 0.0 0.0 0.0 1.225234188199647E-4 0.0 14-15 0.0 0.0 0.0 2.7567769234492054E-4 0.0 16-17 0.0 0.0 0.0 4.2883196586987644E-4 0.0 18-19 0.0 0.0 0.0 5.819862393948323E-4 0.0 20-21 0.0 0.0 0.0 0.0011027107693796822 0.0 22-23 0.0 0.0 0.0 0.001960374701119435 0.0 24-25 0.0 0.0 0.0 0.003308132308139047 0.0 26-27 0.0 6.126170940998235E-5 0.0 0.005115352735733526 0.0 28-29 0.0 6.126170940998235E-5 0.0 0.009832504360302167 0.0 30-31 0.0 6.126170940998235E-5 0.0 0.021012766327623944 0.0 32-33 0.0 6.126170940998235E-5 0.0 0.03501106692780491 0.0 34-35 0.0 6.126170940998235E-5 0.0 0.04836611957918106 0.0 36-37 0.0 6.126170940998235E-5 0.0 0.06380407035049662 0.0 38-39 0.0 6.126170940998235E-5 0.0 0.09048354479854392 0.0 40-41 0.0 6.126170940998235E-5 0.0 0.12123692292235508 0.0 42-43 0.0 6.126170940998235E-5 0.0 0.1486209070286172 0.0 44-45 0.0 6.126170940998235E-5 0.0 0.15918855190183914 0.0 46-47 0.0 6.126170940998235E-5 0.0 0.17505533463902456 0.0 48-49 0.0 6.126170940998235E-5 0.0 0.19631314780428843 0.0 50-51 0.0 6.126170940998235E-5 0.0 0.2172952832772074 0.0 52-53 0.0 6.126170940998235E-5 0.0 0.2667334827710631 0.0 54-55 0.0 6.126170940998235E-5 0.0 0.29865083337366394 0.0 56-57 0.0 6.126170940998235E-5 0.0 0.38640823210346364 0.0 58-59 0.0 6.126170940998235E-5 0.0 0.5468220181935025 0.0 60-61 0.0 6.126170940998235E-5 0.0 0.6534786542762817 0.0 62-63 0.0 6.126170940998235E-5 0.0 0.7104520440275652 0.0 64-65 0.0 6.126170940998235E-5 0.0 0.8421034575496174 0.0 66-67 0.0 6.126170940998235E-5 0.0 1.1982177743498448 0.0 68-69 0.0 6.126170940998235E-5 0.0 1.5765088299564858 0.0 70-71 0.0 6.126170940998235E-5 0.0 1.8105898216120284 0.0 72-73 0.0 6.126170940998235E-5 0.0 2.054564579337283 0.0 74-75 0.0 6.126170940998235E-5 0.0 2.356615437583201 0.0 76-77 0.0 6.126170940998235E-5 0.0 2.468142379564074 0.0 78-79 0.0 6.126170940998235E-5 0.0 2.6672123042918114 0.0 80-81 0.0 6.126170940998235E-5 0.0 2.9431044126196673 0.0 82-83 0.0 6.126170940998235E-5 0.0 3.1501689904254073 0.0 84-85 0.0 6.126170940998235E-5 0.0 3.581910887492258 0.0 86-87 0.0 6.126170940998235E-5 0.0 4.465825461714188 0.0 88-89 0.0 6.126170940998235E-5 0.0 5.585689509728666 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 295 0.0 59.593723 2 TACCTGG 1735 0.0 56.961926 2 ACCTGGG 1920 0.0 49.49214 3 TAACGCA 410 0.0 47.496445 4 GTACCTG 2145 0.0 47.420597 1 ATAACGC 410 0.0 44.035934 3 AGTACGC 1595 0.0 43.79025 2 GTATAAC 470 0.0 43.486168 1 GAGTACG 1645 0.0 43.341694 1 TACGCAG 1645 0.0 43.021095 4 GTACGCA 1685 0.0 41.450035 3 ACGCAGT 1765 0.0 39.288845 5 GTACCCG 200 0.0 38.025112 1 CCTGGGG 1250 0.0 36.857243 4 CGCAGTG 1985 0.0 35.17369 6 TACCCGG 220 0.0 34.555573 2 GTACCGG 90 2.402706E-5 31.687592 1 TCTAACG 75 3.0737265E-4 31.675941 2 GTACACG 155 1.3478711E-9 30.665413 1 TATCACG 220 0.0 30.236126 2 >>END_MODULE