FastQCFastQC Report
Wed 25 May 2016
SRR1003803_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003803_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690688
Sequences flagged as poor quality0
Sequence length121
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT113960.6740451224590226No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93620.5537390695385548No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC76900.4548444183669607No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT72470.42864206760797974No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT47670.28195622137260096No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38460.22748135670212366No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC34070.20151559601771585No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27290.1614135783775599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27050.15999403793012076No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24760.14644925616080554No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24280.14361017526592726No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20660.12219877351705341No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20140.11912310254760192No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC20040.11853162736116893No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC19490.11527851383578756No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA19440.11498277624257107No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT18890.11172966271718969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18730.11078330241889693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16950.041.0660972
TATAACG2950.040.951052
TAACGCA3450.038.2983364
GTACACG2150.037.537631
GTACCTG22200.032.978281
ACCTGGG20850.032.7887653
ATAACGC4050.032.625483
GTATAAC6050.030.491011
TAGACCG800.006363644728.7263245
TAACGCC1404.068781E-628.7237514
ACGTGCG1308.1364444E-526.4976259
TACACGG2951.0913936E-1125.3506492
GTACATG51250.024.0711691
GAGTACT44650.023.99722312-13
GGCGTCG2401.9001163E-823.9264648
AGTACTT50650.023.59323512-13
GTACGAC1250.002003667622.9796873
TACATGG49300.022.403822
TATCACG2854.7275535E-922.2032032
GCGTCGG2604.44943E-822.0813569
Produced by FastQC (version 0.11.5)