FastQCFastQC Report
Wed 25 May 2016
SRR1003803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690688
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT225881.3360241511148123No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT67000.3962883749100958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44130.26101799977287354No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC43380.2565819358746262No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT43190.25545813302040354No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG40330.23854194268842036No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA38620.2284277170004164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36610.21653906575311352No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34270.2026985463905818No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA31600.1869061589128213No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30250.17892124389597608No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27620.16336544649278875No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26410.1562085967369497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25450.1505304349471931No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT19790.1170529393950865No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG18010.10652468107657947No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC17940.10611064844607639No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17810.10534173070371351No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17530.10368560018170118No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2400.041.573682
TACCTGG19200.037.1193582
TAACGCA2600.032.878874
ACCTGGG21100.032.8742033
ATAACGC3050.032.7117843
GTACCTG23850.030.892041
ACGTGCG1004.9593727E-528.4958659
CCTGGGG15350.024.1325934
ACGATCG601.7028354E-423.76834760-61
TCTAACG1302.9369537E-421.9289762
CTAACGC1302.9381292E-421.927683
TCACGCA2005.398251E-721.3712674
CGCGATA704.8552963E-420.35659814-15
AGTACTT56000.020.1845712-13
GTATAAC6200.019.1668871
GAGTACT53300.019.1130812-13
GTACATG66050.018.9991591
TGCGCGA1001.2875496E-518.99724412-13
TACATGG63850.018.3800432
CGCGTTT1051.9605712E-518.09207772-73
Produced by FastQC (version 0.11.5)