##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003802_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2393509 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.81026935766692 31.0 31.0 34.0 28.0 34.0 2 30.71048364555972 31.0 31.0 34.0 27.0 34.0 3 30.791521151581215 31.0 31.0 34.0 27.0 34.0 4 34.2347595099914 37.0 35.0 37.0 30.0 37.0 5 34.200037267459614 37.0 35.0 37.0 30.0 37.0 6 34.32841489211029 37.0 35.0 37.0 30.0 37.0 7 34.37219287665098 37.0 35.0 37.0 30.0 37.0 8 34.33934361642258 37.0 35.0 37.0 30.0 37.0 9 35.78885895143908 39.0 35.0 39.0 30.0 39.0 10-11 35.620793780177976 39.0 35.0 39.0 30.0 39.0 12-13 35.469047536483046 38.5 35.0 39.0 30.0 39.0 14-15 36.41391175048851 39.0 36.0 41.0 29.5 41.0 16-17 36.51549377921704 39.0 36.0 41.0 30.0 41.0 18-19 36.525799359852 39.0 36.0 41.0 30.0 41.0 20-21 36.39860347297629 39.0 36.0 41.0 29.0 41.0 22-23 36.322639689259574 39.0 36.0 41.0 29.0 41.0 24-25 36.09399505078109 39.0 35.5 41.0 27.0 41.0 26-27 35.89561204908776 39.0 35.0 40.5 27.0 41.0 28-29 35.728776244417716 39.0 35.0 40.0 27.0 41.0 30-31 35.53495349296786 38.0 35.0 40.0 27.0 41.0 32-33 35.308802891486934 38.0 34.0 40.0 26.5 41.0 34-35 35.281984943444954 38.0 35.0 40.0 25.5 41.0 36-37 35.13810476584797 38.0 34.5 40.0 25.0 41.0 38-39 35.15070175211373 38.0 34.5 40.0 25.5 41.0 40-41 35.30301891490694 38.5 35.0 40.0 25.5 41.0 42-43 35.16088429164043 38.0 35.0 40.0 25.0 41.0 44-45 34.951215976208985 38.0 34.0 40.0 24.0 41.0 46-47 34.678701229032356 38.0 34.0 40.0 23.5 41.0 48-49 34.40515034620718 38.0 34.0 40.0 23.0 41.0 50-51 33.63185243924297 37.5 32.5 39.5 22.0 40.5 52-53 33.7929000893667 38.0 33.0 39.5 22.0 40.5 54-55 34.14533870564097 38.0 34.0 40.0 22.0 41.0 56-57 33.91517642089501 38.0 33.0 40.0 21.0 41.0 58-59 33.631262301499596 38.0 33.0 40.0 19.0 41.0 60-61 33.28206536929671 37.5 32.0 40.0 18.0 41.0 62-63 33.061719216430774 37.0 32.0 40.0 17.5 41.0 64-65 32.76119893428435 36.5 32.0 39.5 14.0 41.0 66-67 32.32397475839865 36.0 31.0 39.0 13.0 41.0 68-69 31.939177166244207 36.0 31.0 39.0 9.5 40.5 70-71 31.463726687470157 35.0 30.5 38.5 8.0 40.0 72-73 31.069012901142216 35.0 30.0 37.5 7.0 40.0 74-75 30.713761886836437 35.0 30.0 37.0 6.0 39.0 76-77 30.296787269235253 34.5 29.5 36.5 2.0 39.0 78-79 29.731806732291375 34.0 29.0 36.0 2.0 39.0 80-81 29.269809305083037 34.0 29.0 36.0 2.0 37.5 82-83 28.89306871208757 34.0 28.5 35.0 2.0 37.0 84-85 28.629974443379993 34.0 28.0 35.0 2.0 37.0 86-87 28.48581705771735 34.0 28.0 35.0 2.0 36.0 88-89 28.123327298957307 34.0 27.0 35.0 2.0 36.0 90-91 27.73055877375017 34.0 26.0 35.0 2.0 35.5 92-93 27.46353136754447 34.0 26.0 35.0 2.0 35.0 94-95 27.227336935018837 33.5 25.0 35.0 2.0 35.0 96-97 26.933406976953084 33.0 25.0 35.0 2.0 35.0 98-99 26.683395174198218 33.0 25.0 35.0 2.0 35.0 100-101 26.41258608177366 33.0 24.5 35.0 2.0 35.0 102-103 26.020693676104834 33.0 23.5 35.0 2.0 35.0 104-105 25.685520923464253 32.0 21.0 35.0 2.0 35.0 106-107 25.273842713772957 32.0 19.0 35.0 2.0 35.0 108-109 24.989091121027748 32.0 18.5 34.5 2.0 35.0 110-111 24.64848742996162 32.0 16.5 34.0 2.0 35.0 112-113 24.080740870412434 31.5 7.0 34.0 2.0 35.0 114-115 23.552216849821747 31.0 2.0 34.0 2.0 35.0 116-117 22.90427464446551 31.0 2.0 34.0 2.0 35.0 118-119 22.187946650712405 31.0 2.0 34.0 2.0 35.0 120-121 20.537269548599983 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.45514754238482524 1101 2 0.38762283171811873 1101 3 0.49040415298659923 1101 4 0.65203997207783 1101 5 0.6512016398712817 1101 6 0.6860319143836193 1101 7 0.5912759105811958 1101 8 0.5041084456672849 1101 9 0.5945124749062458 1101 10-11 0.7104475294439325 1101 12-13 0.7293902638047598 1101 14-15 0.7931705700437348 1101 16-17 0.7105804456969125 1101 18-19 0.5947217924555233 1101 20-21 0.638446630174009 1101 22-23 0.6890632414240727 1101 24-25 0.7132978003024348 1101 26-27 0.7033844935170634 1101 28-29 0.8446382424738701 1101 30-31 0.7127409210141451 1101 32-33 0.8101048058217515 1101 34-35 0.8370219194991435 1101 36-37 0.7096500887662458 1101 38-39 0.6718766076722247 1101 40-41 0.6970115396528556 1101 42-43 0.7579025753470887 1101 44-45 0.722759737902372 1101 46-47 0.6820243698118702 1101 48-49 0.7536860766119915 1101 50-51 0.5345519089087887 1101 52-53 0.5491978425702939 1101 54-55 0.6108799214275749 1101 56-57 0.630358685558015 1101 58-59 0.6187169684716594 1101 60-61 0.5418609726869477 1101 62-63 0.623088483201613 1101 64-65 0.6286376606989492 1101 66-67 0.6918288806975141 1101 68-69 0.5483902632086206 1101 70-71 0.2989651981755479 1101 72-73 0.2165072875289269 1101 74-75 0.24476801320543018 1101 76-77 0.3391807271542753 1101 78-79 0.6143874863302408 1101 80-81 0.57725239999208 1101 82-83 0.6589322572494574 1101 84-85 0.637003957515109 1101 86-87 0.6415944886744143 1101 88-89 0.7396060192689475 1101 90-91 0.6813708442602469 1101 92-93 0.7514580552969861 1101 94-95 0.6848001960229055 1101 96-97 0.7580185693971018 1101 98-99 0.7267279125469273 1101 100-101 0.6907076646924146 1101 102-103 0.6938590380790295 1101 104-105 0.7890847416553761 1101 106-107 0.7700374759105557 1101 108-109 0.7862867768878168 1101 110-111 0.7218602416424424 1101 112-113 0.6122883772402261 1101 114-115 0.7068741813780548 1101 116-117 0.48626184740616907 1101 118-119 0.419517543764524 1101 120-121 0.5115839637813693 1102 1 0.46302995754427556 1102 2 0.42550910645312356 1102 3 0.4664507456758429 1102 4 0.7232195376062549 1102 5 0.7476702172726917 1102 6 0.672557788897997 1102 7 0.6035130354244416 1102 8 0.5152515789392709 1102 9 0.517638477360876 1102 10-11 0.6718324092562185 1102 12-13 0.6379138715418975 1102 14-15 0.8834011696668469 1102 16-17 0.6851799757813453 1102 18-19 0.6538473626555046 1102 20-21 0.7075764917590277 1102 22-23 0.6378182500802936 1102 24-25 0.723352506558463 1102 26-27 0.6569524529857418 1102 28-29 0.8005736976389102 1102 30-31 0.9547843303325863 1102 32-33 0.6222976145466177 1102 34-35 0.6499996643941088 1102 36-37 0.6804050675964461 1102 38-39 0.545696921949407 1102 40-41 0.6523023967323738 1102 42-43 0.7238479977435759 1102 44-45 0.7162231660868841 1102 46-47 0.6122989895190329 1102 48-49 0.7011624617522259 1102 50-51 0.4194800771773757 1102 52-53 0.5800149062568494 1102 54-55 0.6233931595046229 1102 56-57 0.5756073009853608 1102 58-59 0.6456125231042407 1102 60-61 0.7845440276367128 1102 62-63 0.5660234678278613 1102 64-65 0.5755831872639234 1102 66-67 0.4846538095491084 1102 68-69 0.3614225407454974 1102 70-71 0.3035976904597355 1102 72-73 0.31654892153165193 1102 74-75 0.5051716170785383 1102 76-77 0.3711030784593419 1102 78-79 0.544605792737805 1102 80-81 0.660411451453534 1102 82-83 0.5430216244526811 1102 84-85 0.36939147225561797 1102 86-87 0.5288941034736396 1102 88-89 0.6144345196747132 1102 90-91 0.621697221009132 1102 92-93 0.6034869589326846 1102 94-95 0.5358778945762523 1102 96-97 0.6203726166762351 1102 98-99 0.6657000434733504 1102 100-101 0.7101863905071895 1102 102-103 0.5191084080600774 1102 104-105 0.7148532957746845 1102 106-107 0.5991699038684821 1102 108-109 0.5471780583870931 1102 110-111 0.48807279608048404 1102 112-113 0.49173890856622293 1102 114-115 0.4270111050514984 1102 116-117 0.5507364576758462 1102 118-119 0.45277894965609633 1102 120-121 0.559367300920858 1103 1 0.5839141034263875 1103 2 0.6630398802502704 1103 3 0.6594344705935029 1103 4 0.8693653172643607 1103 5 0.913611420863397 1103 6 0.8158702698762497 1103 7 0.8623971330930118 1103 8 0.7863984886021669 1103 9 0.9214351427920775 1103 10-11 0.9081299711578765 1103 12-13 0.8648979339543175 1103 14-15 1.049176639221507 1103 16-17 0.980842339389767 1103 18-19 0.8601866142005932 1103 20-21 0.9406041964631626 1103 22-23 1.0440331257646989 1103 24-25 1.0641837983498093 1103 26-27 1.094584851880434 1103 28-29 1.1436638690079022 1103 30-31 1.1321035521660932 1103 32-33 1.0602038512690655 1103 34-35 0.9519211245410943 1103 36-37 0.8826077482691517 1103 38-39 0.9119936250474225 1103 40-41 1.1028499970542 1103 42-43 1.032904684810596 1103 44-45 0.96288061191445 1103 46-47 0.7830453501502674 1103 48-49 1.0924861342979284 1103 50-51 1.021487493022157 1103 52-53 1.0097421683004413 1103 54-55 0.9891862571340297 1103 56-57 1.0745288997462765 1103 58-59 1.0497568533428634 1103 60-61 1.0469770936862375 1103 62-63 1.2262140770360404 1103 64-65 1.1728396862981398 1103 66-67 1.0879249474187347 1103 68-69 0.9783629699062466 1103 70-71 0.9809655024992558 1103 72-73 0.8936088449323982 1103 74-75 0.9348792923724147 1103 76-77 0.9780109484153776 1103 78-79 1.2036648904817149 1103 80-81 1.1551704892318497 1103 82-83 1.1556450586427083 1103 84-85 1.1706823897291052 1103 86-87 1.183223377829762 1103 88-89 1.2061686852942444 1103 90-91 1.2515505915874208 1103 92-93 0.9827391669620482 1103 94-95 1.24506061484432 1103 96-97 1.0621901766493558 1103 98-99 1.1791828839739225 1103 100-101 1.2348174816096815 1103 102-103 1.1062980710775818 1103 104-105 1.089436700674991 1103 106-107 1.027256398955224 1103 108-109 1.015499504070025 1103 110-111 1.1405797493766343 1103 112-113 1.1934910939954904 1103 114-115 1.1416886619510045 1103 116-117 0.9550152584846288 1103 118-119 0.9500680681619578 1103 120-121 0.934630968705477 1104 1 0.5999598073726204 1104 2 0.8346731586745584 1104 3 0.8633056264493568 1104 4 1.0510037595914739 1104 5 1.0961936720252723 1104 6 0.9741727764810051 1104 7 0.8157556455619996 1104 8 0.9327534408394769 1104 9 0.9879968963935184 1104 10-11 1.0471004061110918 1104 12-13 1.079217954228433 1104 14-15 1.2833124212530365 1104 16-17 1.198408874029468 1104 18-19 1.060620981540751 1104 20-21 1.0372706860038718 1104 22-23 1.164026149727114 1104 24-25 1.2297366833909749 1104 26-27 1.130798593856973 1104 28-29 1.0623543359527972 1104 30-31 1.057481750141136 1104 32-33 1.163656581120648 1104 34-35 0.9779821430698377 1104 36-37 1.0284408601950403 1104 38-39 0.8444837025877163 1104 40-41 0.8896489359334794 1104 42-43 0.8625576814411176 1104 44-45 0.8063035846057218 1104 46-47 0.951793655531759 1104 48-49 1.0788118743778554 1104 50-51 0.8932007506273294 1104 52-53 0.8769911024790673 1104 54-55 0.732561882695208 1104 56-57 0.9066207845162992 1104 58-59 0.973804222273067 1104 60-61 0.8208007389111316 1104 62-63 0.9270465008957274 1104 64-65 1.002834399769661 1104 66-67 0.9381146084405358 1104 68-69 0.9069598189717745 1104 70-71 0.8396852088516731 1104 72-73 0.7083675244447818 1104 74-75 0.8486993405779231 1104 76-77 0.8110663983766138 1104 78-79 1.0111108568468552 1104 80-81 1.098559126738138 1104 82-83 1.1067338382585064 1104 84-85 1.116200421832506 1104 86-87 1.1481430375844326 1104 88-89 1.1824643957123833 1104 90-91 1.3149331386858947 1104 92-93 1.1367320628487754 1104 94-95 1.1957447979053555 1104 96-97 1.2775912102474258 1104 98-99 1.2120113402122321 1104 100-101 1.098760291726574 1104 102-103 1.2170835429343114 1104 104-105 1.2657636806365922 1104 106-107 1.2123118053162223 1104 108-109 1.2358813195020524 1104 110-111 1.2260828075243602 1104 112-113 1.1394779648747573 1104 114-115 1.2235548473800826 1104 116-117 1.080546193532541 1104 118-119 1.0613271475702497 1104 120-121 1.2004407545056992 1105 1 0.5782771604594892 1105 2 0.686954638427153 1105 3 0.8287316579974551 1105 4 1.0313714371933642 1105 5 1.0097288006846838 1105 6 1.0140886547444197 1105 7 0.859756504080309 1105 8 0.8435574693468482 1105 9 1.1049644661093723 1105 10-11 0.962008184760272 1105 12-13 1.1904356547214263 1105 14-15 1.2654074180988815 1105 16-17 1.210547686319991 1105 18-19 0.9137135652320012 1105 20-21 1.1686533656418945 1105 22-23 1.199472183445998 1105 24-25 1.272228769435408 1105 26-27 1.27342117141945 1105 28-29 1.3005913702453853 1105 30-31 1.1883906941554585 1105 32-33 1.2798655209729475 1105 34-35 1.3043896601647162 1105 36-37 1.3809684702906324 1105 38-39 1.247907411566338 1105 40-41 1.236553577563292 1105 42-43 1.3109346166776632 1105 44-45 1.2088087932493394 1105 46-47 1.3793279370980187 1105 48-49 1.486523768039099 1105 50-51 1.3739279939756486 1105 52-53 1.5550372715043466 1105 54-55 1.6002931827873468 1105 56-57 1.589825326502499 1105 58-59 1.6881987669278544 1105 60-61 1.6820710358915463 1105 62-63 1.6658110506183803 1105 64-65 1.6466135287979498 1105 66-67 1.4344299164258203 1105 68-69 1.4497028502432165 1105 70-71 1.2120212518494036 1105 72-73 1.23581677026257 1105 74-75 1.267692997172233 1105 76-77 1.3315193728732346 1105 78-79 1.4065855187236664 1105 80-81 1.4854263265239993 1105 82-83 1.5323988839142153 1105 84-85 1.3255202419353793 1105 86-87 1.4710571522945557 1105 88-89 1.3270823754686525 1105 90-91 1.4524077891375562 1105 92-93 1.5143622748587262 1105 94-95 1.571252135707013 1105 96-97 1.4908250414180024 1105 98-99 1.5787848864232856 1105 100-101 1.4685314704836294 1105 102-103 1.3982945597154064 1105 104-105 1.6618872909508902 1105 106-107 1.7573860565405504 1105 108-109 1.9018740384181 1105 110-111 1.8661119579747982 1105 112-113 1.627891150475481 1105 114-115 1.5201648425896792 1105 116-117 1.693021394839576 1105 118-119 1.4178214060250625 1105 120-121 1.4455775992224247 1106 1 0.5994574772872845 1106 2 0.6903436093882824 1106 3 0.655773268956338 1106 4 0.8034656957670165 1106 5 0.954080081445909 1106 6 0.8582080174005924 1106 7 0.8399721057344038 1106 8 0.8040756670004114 1106 9 0.9349444413741139 1106 10-11 1.1362691838321908 1106 12-13 1.0146499494558654 1106 14-15 1.1695026668846609 1106 16-17 0.9833683677222425 1106 18-19 0.8782963431578921 1106 20-21 1.071349589004889 1106 22-23 0.8585147846170074 1106 24-25 1.138933387552278 1106 26-27 1.1545114270505294 1106 28-29 1.2067928242937285 1106 30-31 1.2171245856690973 1106 32-33 1.3001494352374152 1106 34-35 1.1134539096981726 1106 36-37 1.100532576503909 1106 38-39 1.1254470350852799 1106 40-41 1.1949506365931697 1106 42-43 1.0649206214122415 1106 44-45 1.3261166590214728 1106 46-47 1.3080213381559815 1106 48-49 1.3357390149005894 1106 50-51 1.0031016679633424 1106 52-53 1.1970773891271236 1106 54-55 1.2467205014171654 1106 56-57 1.1567223745077442 1106 58-59 1.2749878537478097 1106 60-61 1.2335414207312851 1106 62-63 1.191890311687608 1106 64-65 1.0683456784202008 1106 66-67 1.1004692482932086 1106 68-69 0.9995843855931916 1106 70-71 0.7437367041698941 1106 72-73 0.7935509484165628 1106 74-75 0.8806928388184723 1106 76-77 1.0684794416669199 1106 78-79 1.155529459487333 1106 80-81 1.001164585795209 1106 82-83 1.0901634299099712 1106 84-85 1.1094176687869641 1106 86-87 1.0345792606846977 1106 88-89 0.9150025892261873 1106 90-91 1.0243516116370195 1106 92-93 0.8797794167537205 1106 94-95 0.7704882291397404 1106 96-97 0.8920641094960899 1106 98-99 1.1248769561069594 1106 100-101 1.0243557819955456 1106 102-103 0.8903428956979909 1106 104-105 0.9935855047605422 1106 106-107 1.035118109947863 1106 108-109 1.1151963003512613 1106 110-111 0.91179882242999 1106 112-113 0.6968936019520804 1106 114-115 0.7715455270387359 1106 116-117 0.9601404387837462 1106 118-119 1.0683727377000274 1106 120-121 0.7512452607783402 1107 1 0.4014998323882537 1107 2 0.5858926708696792 1107 3 0.5641108171935727 1107 4 0.87516729930379 1107 5 0.7571770991672366 1107 6 0.7119072980569925 1107 7 0.6531305673881391 1107 8 0.6934007203891923 1107 9 0.8007470527412366 1107 10-11 0.7204157594569338 1107 12-13 0.7949387172052909 1107 14-15 0.8946359046801788 1107 16-17 0.7913380485135235 1107 18-19 0.7541208251930342 1107 20-21 0.6521080843778648 1107 22-23 0.6250095768690827 1107 24-25 0.7183154826405129 1107 26-27 0.7400074744073208 1107 28-29 0.7714342296363483 1107 30-31 0.911238004049828 1107 32-33 0.8059212778060783 1107 34-35 0.8759457140517029 1107 36-37 0.8405851691381301 1107 38-39 0.8105054349204224 1107 40-41 0.7767267970967069 1107 42-43 0.86160396036545 1107 44-45 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1.3756066937024158 2204 68-69 1.2876632201479374 2204 70-71 1.245076868645274 2204 72-73 1.1958165021293148 2204 74-75 1.4201580355748717 2204 76-77 1.2338510894623376 2204 78-79 1.4552204225234142 2204 80-81 1.5827266715221207 2204 82-83 1.2711566523305002 2204 84-85 1.3187036987093173 2204 86-87 1.2297886138591423 2204 88-89 1.1836958822345487 2204 90-91 1.3060710852698811 2204 92-93 1.4417443409148447 2204 94-95 1.4027571974670394 2204 96-97 1.4893242399974937 2204 98-99 1.51686695903377 2204 100-101 1.4292789962095256 2204 102-103 1.407878089183253 2204 104-105 1.4919846531155017 2204 106-107 1.4981220525364272 2204 108-109 1.300717043417297 2204 110-111 1.3717442459856635 2204 112-113 1.6106427339513196 2204 114-115 1.8560903790051526 2204 116-117 1.7520516760219849 2204 118-119 1.819007597758219 2204 120-121 1.7583259948917735 2205 1 1.1173828709579752 2205 2 1.3071387695865901 2205 3 1.3796595976813784 2205 4 1.1820272962212997 2205 5 1.3312460892647167 2205 6 1.2863182524745937 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2205 72-73 2.2719267246558097 2205 74-75 2.3050616552947645 2205 76-77 2.265488964020424 2205 78-79 2.3486861779154147 2205 80-81 2.3393812193374828 2205 82-83 2.2637252487668498 2205 84-85 2.337019935805987 2205 86-87 2.350084935512342 2205 88-89 2.35840137053966 2205 90-91 2.4162825454537504 2205 92-93 2.445047760752381 2205 94-95 2.463368668274576 2205 96-97 2.505880440186754 2205 98-99 2.5841423225501288 2205 100-101 2.592244593230177 2205 102-103 2.5110587466309333 2205 104-105 2.7031622769156662 2205 106-107 2.7862794657912247 2205 108-109 2.827450324419228 2205 110-111 2.8931787264784425 2205 112-113 2.8638374579228447 2205 114-115 2.967378223364477 2205 116-117 3.070329648239124 2205 118-119 2.97218368112647 2205 120-121 2.958039401764502 2206 1 0.6750442070535563 2206 2 0.9666798769453351 2206 3 1.131655605665408 2206 4 1.1317090937636536 2206 5 1.2150844551869042 2206 6 1.2685835796401648 2206 7 1.094695608038549 2206 8 1.082384947057804 2206 9 1.1651432603260687 2206 10-11 1.3046482091916118 2206 12-13 1.316505340057148 2206 14-15 1.608567796993114 2206 16-17 1.3892824037055718 2206 18-19 1.374115591757466 2206 20-21 1.486664581140431 2206 22-23 1.423175440264238 2206 24-25 1.5662370176193363 2206 26-27 1.6007570119176648 2206 28-29 1.5984737481602735 2206 30-31 1.456192302769459 2206 32-33 1.4198428249556798 2206 34-35 1.5588482017592113 2206 36-37 1.5754751452833986 2206 38-39 1.585852606431061 2206 40-41 1.5893272060349304 2206 42-43 1.5864712988591876 2206 44-45 1.5163154089869764 2206 46-47 1.5058808610456893 2206 48-49 1.56443099729524 2206 50-51 1.6659443581589883 2206 52-53 1.4783572257672049 2206 54-55 1.3361652294725772 2206 56-57 1.3896656469490338 2206 58-59 1.4531413968919367 2206 60-61 1.4713496177013923 2206 62-63 1.5606599728261656 2206 64-65 1.6381530682488332 2206 66-67 1.580353489028326 2206 68-69 1.5204926267146313 2206 70-71 1.4179819923952799 2206 72-73 1.5058200162716382 2206 74-75 1.5452796789630696 2206 76-77 1.4721600412971512 2206 78-79 1.5579072499892774 2206 80-81 1.607063272293182 2206 82-83 1.5454550356386072 2206 84-85 1.6145238734283751 2206 86-87 1.525677766438168 2206 88-89 1.543440356548686 2206 90-91 1.6472563206521684 2206 92-93 1.6888090533330953 2206 94-95 1.772414675785619 2206 96-97 1.7410617363621732 2206 98-99 1.815566027273384 2206 100-101 1.7208647226396074 2206 102-103 1.7263148458069892 2206 104-105 1.6509473938855166 2206 106-107 1.7121288925301634 2206 108-109 1.823409827214583 2206 110-111 1.85640925594085 2206 112-113 1.9728951127553565 2206 114-115 1.9564897514930557 2206 116-117 1.9016119887713288 2206 118-119 2.0218379271368185 2206 120-121 1.9083425868634976 2207 1 0.5223850390332565 2207 2 0.9622873778344072 2207 3 0.8978511439628107 2207 4 1.0423747207760812 2207 5 1.204025713921439 2207 6 1.098160381093443 2207 7 0.9761161537849361 2207 8 0.9894456250597514 2207 9 1.1462192303763388 2207 10-11 1.252040943786632 2207 12-13 1.2491034263884941 2207 14-15 1.376940662017482 2207 16-17 1.2513821408284471 2207 18-19 1.1653982952109132 2207 20-21 1.3474045778724886 2207 22-23 1.1732949844823892 2207 24-25 1.3863923530159568 2207 26-27 1.439781711315014 2207 28-29 1.4198746862519869 2207 30-31 1.318611925802685 2207 32-33 1.3330197816362173 2207 34-35 1.4159366933154232 2207 36-37 1.3404461062507096 2207 38-39 1.3197345616218072 2207 40-41 1.2764307277234934 2207 42-43 1.3075007487875823 2207 44-45 1.3113066864641283 2207 46-47 1.371328437001317 2207 48-49 1.3998189092722555 2207 50-51 1.251726946273287 2207 52-53 1.3499847572211507 2207 54-55 1.3675212573846451 2207 56-57 1.2540247097223656 2207 58-59 1.3403207901119103 2207 60-61 1.3687185035252512 2207 62-63 1.0078691860209616 2207 64-65 1.1613206175803512 2207 66-67 1.1851751458751423 2207 68-69 1.1403224894610524 2207 70-71 1.1010840867619258 2207 72-73 0.9835796855366361 2207 74-75 1.0966963079843168 2207 76-77 1.1037743207639004 2207 78-79 1.2028149666586678 2207 80-81 1.1382522987346775 2207 82-83 1.037769964368625 2207 84-85 1.063717881079203 2207 86-87 0.9180528277120885 2207 88-89 0.9681326052775496 2207 90-91 1.0867810633017 2207 92-93 1.133842741975073 2207 94-95 1.2133376052076734 2207 96-97 1.1415540903380084 2207 98-99 1.1327706028733857 2207 100-101 1.1734561289121324 2207 102-103 1.1256351858652387 2207 104-105 1.2740196682773437 2207 106-107 1.239401494196816 2207 108-109 1.3919477113179646 2207 110-111 1.3335018204106248 2207 112-113 1.1525439411018716 2207 114-115 1.1786650838139678 2207 116-117 1.1146788326117374 2207 118-119 1.09527171766171 2207 120-121 1.0344599348320855 2208 1 0.510637033363718 2208 2 0.7460880521766171 2208 3 0.854146379545206 2208 4 1.0248617008224628 2208 5 1.126393151485047 2208 6 0.9652107064233775 2208 7 0.7795095203114784 2208 8 0.7356095125480024 2208 9 0.9055413962650931 2208 10-11 0.9843474615508754 2208 12-13 1.0947244335493806 2208 14-15 1.2373851788443844 2208 16-17 1.1214583652516907 2208 18-19 1.1903570289097445 2208 20-21 1.2875435498313195 2208 22-23 1.1878670915208787 2208 24-25 1.3105589906427113 2208 26-27 1.354775531148583 2208 28-29 1.529186646687549 2208 30-31 1.4293612727603602 2208 32-33 1.2839430195598212 2208 34-35 1.1977279245909145 2208 36-37 1.2277795031162455 2208 38-39 1.2046276283853814 2208 40-41 1.101342221197335 2208 42-43 1.1514524353898494 2208 44-45 1.159626538168574 2208 46-47 1.0516054563831503 2208 48-49 1.1190794264007096 2208 50-51 0.9904831682216226 2208 52-53 1.0137773027598769 2208 54-55 0.9257144761276876 2208 56-57 0.7287350988737202 2208 58-59 0.7674061162348167 2208 60-61 0.7959716684603109 2208 62-63 0.8467755739234804 2208 64-65 0.8289838568267598 2208 66-67 0.777128857210883 2208 68-69 0.8748854969334339 2208 70-71 0.7195020139383779 2208 72-73 0.6710823969157538 2208 74-75 0.6642467183004328 2208 76-77 0.7299268791323357 2208 78-79 0.7924384921129182 2208 80-81 0.7753731412570701 2208 82-83 0.7312467773656088 2208 84-85 0.9170886320369895 2208 86-87 0.8917153799572546 2208 88-89 0.9630625930238956 2208 90-91 0.8775157563664742 2208 92-93 0.9578545909685019 2208 94-95 1.147282187107681 2208 96-97 1.303007738023009 2208 98-99 1.21312947923521 2208 100-101 1.1938093407140435 2208 102-103 1.3298916421478495 2208 104-105 1.228955036111035 2208 106-107 1.2349339414929368 2208 108-109 1.1952788494713538 2208 110-111 1.3424024335201175 2208 112-113 1.4387771225505652 2208 114-115 1.4747706188677405 2208 116-117 1.4297946022771484 2208 118-119 1.3485099044051445 2208 120-121 1.3521852957744294 2209 1 0.46626796971930773 2209 2 0.7243422591872175 2209 3 0.8271118269807403 2209 4 0.8862574810654849 2209 5 1.0382856642337757 2209 6 1.0366321957836746 2209 7 0.8513304275714546 2209 8 0.7512084465556228 2209 9 1.0430241228049297 2209 10-11 1.056958079896063 2209 12-13 1.1063706730853582 2209 14-15 1.34721701130335 2209 16-17 1.275487302824537 2209 18-19 1.1340904668480505 2209 20-21 1.2197728868220636 2209 22-23 1.2039691077064276 2209 24-25 1.1623143692854327 2209 26-27 1.2880047298575832 2209 28-29 1.231481935607036 2209 30-31 1.188180873398899 2209 32-33 1.1825107216463664 2209 34-35 1.3391302332376611 2209 36-37 1.4668127725765814 2209 38-39 1.3809901974909806 2209 40-41 1.5193119817065153 2209 42-43 1.5838723566162898 2209 44-45 1.6562280995294572 2209 46-47 1.434413366099271 2209 48-49 1.5626437278850247 2209 50-51 1.3857100020304784 2209 52-53 1.430125027742875 2209 54-55 1.363151861304452 2209 56-57 1.2959007086727254 2209 58-59 1.2543581512812452 2209 60-61 1.2276768517682939 2209 62-63 1.2381043674763603 2209 64-65 1.41668581167918 2209 66-67 1.4591452685266546 2209 68-69 1.3462988963409863 2209 70-71 1.4803105200533473 2209 72-73 1.2618495972370916 2209 74-75 1.3339934933724678 2209 76-77 1.2442125127872892 2209 78-79 1.0253426816736813 2209 80-81 0.9245431215119382 2209 82-83 1.024019721553401 2209 84-85 1.2598897499555761 2209 86-87 1.2602712387292527 2209 88-89 1.309512749978385 2209 90-91 1.5426590661166522 2209 92-93 1.6294580101964407 2209 94-95 1.5202402030749909 2209 96-97 1.4760500400096568 2209 98-99 1.6777427089418921 2209 100-101 1.7727340731106374 2209 102-103 1.899432781909308 2209 104-105 1.8860928672699622 2209 106-107 1.8489214697960143 2209 108-109 1.9074849889929375 2209 110-111 1.8285393587924723 2209 112-113 1.9023173987566047 2209 114-115 1.9531187069579197 2209 116-117 2.144955079659617 2209 118-119 2.2602480495671173 2209 120-121 2.1805592999341705 2210 1 0.3210450397830016 2210 2 0.45470319336980936 2210 3 0.5753071560677867 2210 4 0.6569581180081556 2210 5 0.8000691037242262 2210 6 0.8120037456775151 2210 7 0.6683155655757034 2210 8 0.7287031386999985 2210 9 0.8706249720618331 2210 10-11 0.8726693325499895 2210 12-13 0.9507443461214535 2210 14-15 1.1140959921950682 2210 16-17 0.8990541818054325 2210 18-19 0.7631775156803187 2210 20-21 0.8947198082657977 2210 22-23 0.9254128444367851 2210 24-25 1.0716561951877637 2210 26-27 1.2780259612800862 2210 28-29 1.231481935607036 2210 30-31 1.1627031663925322 2210 32-33 1.1343153925593228 2210 34-35 1.0820177066983732 2210 36-37 1.081674768330302 2210 38-39 1.133856439529204 2210 40-41 1.0568915995409114 2210 42-43 1.1758044160642669 2210 44-45 1.1895614328627957 2210 46-47 1.1482137907277234 2210 48-49 0.7974632607937266 2210 50-51 0.5761558619030822 2210 52-53 0.8751356434541222 2210 54-55 0.9899034536611353 2210 56-57 0.8525886067618913 2210 58-59 0.7040396799436692 2210 60-61 0.8009252594115992 2210 62-63 0.7572126477311301 2210 64-65 0.698214474099558 2210 66-67 0.9183809373164635 2210 68-69 1.112116306107442 2210 70-71 1.2376353608176736 2210 72-73 1.2563294273857117 2210 74-75 1.0246516674701311 2210 76-77 0.9703271624688199 2210 78-79 0.7208840829475633 2210 80-81 0.51350278180918 2210 82-83 0.7469496578591333 2210 84-85 0.9460893253271259 2210 86-87 0.9254517058205494 2210 88-89 1.0831857860718053 2210 90-91 1.2382004673905378 2210 92-93 1.2171437851433602 2210 94-95 1.2905162115675566 2210 96-97 1.1915489784385294 2210 98-99 1.0342182928060097 2210 100-101 1.0574474489068173 2210 102-103 1.0824476439050592 2210 104-105 1.2190015721531857 2210 106-107 1.2680297500507898 2210 108-109 1.4871028233878398 2210 110-111 1.5867134564570122 2210 112-113 1.5475403286929073 2210 114-115 1.5138405753231012 2210 116-117 1.1300930839059014 2210 118-119 1.328400915809155 2210 120-121 1.3958459241379941 2211 1 0.33098334590647127 2211 2 0.5496781202873429 2211 3 0.5866902900439754 2211 4 0.6946418471006623 2211 5 0.8141418543072945 2211 6 0.729163917892997 2211 7 0.5903594196017181 2211 8 0.6127732069406093 2211 9 0.7712591869311041 2211 10-11 0.7510251020867642 2211 12-13 0.803596949130224 2211 14-15 0.8985965991318778 2211 16-17 0.8608962691224988 2211 18-19 0.7375247744627202 2211 20-21 0.7904330613107149 2211 22-23 0.8838806358687492 2211 24-25 0.9019584815437369 2211 26-27 0.9867463020739677 2211 28-29 1.0594083030333934 2211 30-31 1.0415431214746604 2211 32-33 0.9540033315655876 2211 34-35 0.8930353146853989 2211 36-37 0.765182908557712 2211 38-39 0.8553160884605759 2211 40-41 0.7064240081480406 2211 42-43 0.8363985321869691 2211 44-45 0.8694711424878605 2211 46-47 0.8759946751733239 2211 48-49 0.7759658772142117 2211 50-51 0.5988352947013169 2211 52-53 0.7463950259697967 2211 54-55 0.6431897778063131 2211 56-57 0.5944067803139959 2211 58-59 0.39366674341516017 2211 60-61 0.5032125246498111 2211 62-63 0.5690767848124665 2211 64-65 0.6719020584910496 2211 66-67 0.641200852117997 2211 68-69 0.8285145112111643 2211 70-71 0.641767077471382 2211 72-73 0.756075741438007 2211 74-75 0.8444669040845625 2211 76-77 0.6497063138484087 2211 78-79 0.40351296040997653 2211 80-81 0.31323545683202525 2211 82-83 0.3559964260911066 2211 84-85 0.585445654064106 2211 86-87 0.7634368574638231 2211 88-89 0.8625094720649749 2211 90-91 0.927549294661322 2211 92-93 0.8821032999576204 2211 94-95 0.9969420129741948 2211 96-97 1.1397343055706521 2211 98-99 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0.21905155479681326 2312 8 0.12548059346357832 2312 9 0.29710636288865544 2312 10-11 0.2057302764868183 2312 12-13 0.23077185854443627 2312 14-15 0.43167932940274767 2312 16-17 0.2191579413807645 2312 18-19 0.38648412969232737 2312 20-21 0.3816401945336523 2312 22-23 0.3559748800279081 2312 24-25 0.3608286255156017 2312 26-27 0.40195064761749677 2312 28-29 0.556329719343708 2312 30-31 0.46537327530271 2312 32-33 0.4241018841237292 2312 34-35 0.6046213773916307 2312 36-37 0.430575137441636 2312 38-39 0.3629422121059278 2312 40-41 0.38237705524162635 2312 42-43 0.4135388254633412 2312 44-45 0.5197958354247874 2312 46-47 0.4331124058993012 2312 48-49 0.44975810499483515 2312 50-51 0.3395278748953743 2312 52-53 0.24353747068741427 2312 54-55 0.32970621748366824 2312 56-57 0.15977583983077182 2312 58-59 0.15376615103328817 2312 60-61 0.15128721459862504 2312 62-63 0.22192436439510743 2312 64-65 0.15958358634532033 2312 66-67 0.3221893750064808 2312 68-69 0.2821149113424575 2312 70-71 0.30167221178475856 2312 72-73 0.3259859049751235 2312 74-75 0.1515727283302013 2312 76-77 0.15446430888906093 2312 78-79 0.1358776241829247 2312 80-81 0.13775719788124974 2312 82-83 0.20865488111725128 2312 84-85 0.1838377735371317 2312 86-87 0.3149123875780262 2312 88-89 0.34698443305861204 2312 90-91 0.31882235025033623 2312 92-93 0.4408598037001177 2312 94-95 0.28102953066152736 2312 96-97 0.3766140537968603 2312 98-99 0.4597528843449865 2312 100-101 0.5046744646840686 2312 102-103 0.4918924818616297 2312 104-105 0.4220014107980212 2312 106-107 0.5190785130401103 2312 108-109 0.6630791360850345 2312 110-111 0.7049797727003728 2312 112-113 0.611247085280894 2312 114-115 0.6684672295553931 2312 116-117 0.5489383643584986 2312 118-119 0.551550185745409 2312 120-121 0.7617729095585943 2313 1 -0.7839472711429316 2313 2 -0.4013722393734085 2313 3 -0.24836880666281758 2313 4 -0.07954290099370098 2313 5 -2.835196874384529 2313 6 -7.822127229318358 2313 7 -3.2201540160216027 2313 8 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2313 70-71 -0.9163799854035055 2313 72-73 -0.6597503154959092 2313 74-75 -0.5640696049252938 2313 76-77 -0.34129903678728013 2313 78-79 -0.24800895644497345 2313 80-81 -0.46522854757585463 2313 82-83 -0.4640850946271762 2313 84-85 -0.3819491892182647 2313 86-87 -0.19926880032804917 2313 88-89 -0.3106448627370533 2313 90-91 -0.2893277480525285 2313 92-93 -0.8209940222200558 2313 94-95 -0.42778298653384894 2313 96-97 -0.4074588915549242 2313 98-99 -0.2611472441787015 2313 100-101 -0.13057994441725995 2313 102-103 -0.246247235544093 2313 104-105 -0.11555562764035088 2313 106-107 -0.12714103253199127 2313 108-109 -0.023245233591122627 2313 110-111 0.10847060942435505 2313 112-113 0.28237343055584674 2313 114-115 0.04122644977568868 2313 116-117 -0.07457942539282314 2313 118-119 -0.005171337541842291 2313 120-121 -0.14743558111133126 2314 1 -0.40771469495179957 2314 2 -0.18438620473300205 2314 3 -0.22625972819236395 2314 4 -0.7004291060075474 2314 5 -0.15384454342589748 2314 6 -0.12693123408092077 2314 7 -0.151833608704095 2314 8 -0.18218806191802628 2314 9 -0.11439143323748624 2314 10-11 -2.661369661821432 2314 12-13 -1.0060071670463415 2314 14-15 -0.02230925433315889 2314 16-17 0.0477637987687487 2314 18-19 -0.1936987639902199 2314 20-21 0.12695259194021702 2314 22-23 0.0850420820325084 2314 24-25 0.27112079027295266 2314 26-27 0.1665465631508809 2314 28-29 0.15564132349447846 2314 30-31 0.24531205332223038 2314 32-33 0.04116707967872202 2314 34-35 0.16212308072072545 2314 36-37 0.049235741579416015 2314 38-39 -0.04538683139448807 2314 40-41 -0.04423094703316366 2314 42-43 -0.1876697063248045 2314 44-45 -0.12115493627360507 2314 46-47 -0.04644773394005597 2314 48-49 -0.1013268208852196 2314 50-51 0.017215215739305734 2314 52-53 0.01059651842101772 2314 54-55 -0.13723220483411325 2314 56-57 -0.233475115661669 2314 58-59 -0.2876551647713086 2314 60-61 -0.18400562188161018 2314 62-63 -0.10737003119397315 2314 64-65 -0.10639616730409074 2314 66-67 -0.08809442657850042 2314 68-69 0.03628106124218178 2314 70-71 0.184266973200355 2314 72-73 0.007973784781032833 2314 74-75 0.09819958511016225 2314 76-77 -0.09192560320332177 2314 78-79 -0.1376411734704739 2314 80-81 -0.1639898866189995 2314 82-83 -0.04534631855313265 2314 84-85 0.032184899283834056 2314 86-87 -0.24196083224511256 2314 88-89 -0.13974846162929921 2314 90-91 -0.06232816247440809 2314 92-93 -0.04308139287767432 2314 94-95 0.21603949618212326 2314 96-97 0.22530905192582296 2314 98-99 0.1674060945486957 2314 100-101 0.16735978680054941 2314 102-103 0.2191649826654718 2314 104-105 0.1455433139393172 2314 106-107 0.33356947076035937 2314 108-109 0.33677555328420183 2314 110-111 0.3713949807200514 2314 112-113 0.6545785496528005 2314 114-115 0.4755340034367421 2314 116-117 0.3333597137937261 2314 118-119 0.5232198504017695 2314 120-121 0.6127934034234954 2315 1 -0.5481187831517289 2315 2 -3.101624543072848 2315 3 -0.975944751963123 2315 4 -0.41990709043006547 2315 5 -3.1953921136327956 2315 6 -0.9634141563808925 2315 7 -0.6069459645252309 2315 8 -0.32514315858786347 2315 9 -0.19577636842145552 2315 10-11 -0.057246727409470566 2315 12-13 0.04081565919302932 2315 14-15 -0.20044020644792937 2315 16-17 -0.21312578180545927 2315 18-19 -0.056555453229101715 2315 20-21 -0.10058876661825167 2315 22-23 0.037692172170572746 2315 24-25 -0.008377476729485522 2315 26-27 -0.21133062272522096 2315 28-29 0.03149259127705051 2315 30-31 -0.06898413231520095 2315 32-33 -0.11794241072102807 2315 34-35 0.042282446819911 2315 36-37 -0.08335438824678221 2315 38-39 -0.2561874351920892 2315 40-41 -0.2075938514736393 2315 42-43 -0.20005383155751844 2315 44-45 -0.1643325485483942 2315 46-47 -0.3199848948953772 2315 48-49 -0.09122201600062141 2315 50-51 -0.5359820118112353 2315 52-53 -0.59044494404624 2315 54-55 -0.43078346410639057 2315 56-57 -0.320251955111587 2315 58-59 -0.5593658189148485 2315 60-61 -0.6160188542630891 2315 62-63 -0.7085610737882604 2315 64-65 -0.3544032244355506 2315 66-67 -0.4622598413998986 2315 68-69 -0.49383168889505313 2315 70-71 -0.47230424817251304 2315 72-73 -0.30721459518299454 2315 74-75 -0.30867473967769143 2315 76-77 -0.412998045916126 2315 78-79 -0.48739244234500134 2315 80-81 -0.34307456370358125 2315 82-83 -0.3011990526715991 2315 84-85 -0.29226895559363086 2315 86-87 -0.22684925292335834 2315 88-89 -0.30702644823572456 2315 90-91 -0.2968614820110105 2315 92-93 -0.12753410727166425 2315 94-95 -0.015174981787566821 2315 96-97 -0.06483869614796234 2315 98-99 -0.09600598240863434 2315 100-101 -0.028955116978526263 2315 102-103 0.12659568312858127 2315 104-105 0.4163712200099319 2315 106-107 0.3147444737892293 2315 108-109 0.38118386508613966 2315 110-111 0.3359506436797979 2315 112-113 0.06517972483608858 2315 114-115 0.4217883731956995 2315 116-117 0.6588601962459677 2315 118-119 0.5769012634503916 2315 120-121 0.35084090265349843 2316 1 -1.7663694300027437 2316 2 -3.8822636627587386 2316 3 -5.9740116580549625 2316 4 -2.3862551342259266 2316 5 -3.223921606531963 2316 6 -1.1655494059531009 2316 7 -1.2511012213314956 2316 8 -1.1534568861718526 2316 9 -1.1640266226863787 2316 10-11 -0.8512219965795751 2316 12-13 -0.6145091953276491 2316 14-15 -5.225035724999195 2316 16-17 -1.707054568782489 2316 18-19 -1.0812370789961463 2316 20-21 -0.779385296575974 2316 22-23 -1.3221035574349784 2316 24-25 -1.2475291452988913 2316 26-27 -1.27571212427803 2316 28-29 -0.9954838310492633 2316 30-31 -0.6968261284531962 2316 32-33 -1.2288843608479354 2316 34-35 -1.1855771371749313 2316 36-37 -1.4674208368449584 2316 38-39 -1.2080406770895067 2316 40-41 -0.9572019196935955 2316 42-43 -0.9826742023786394 2316 44-45 -1.4329614128387078 2316 46-47 -1.3912620402605853 2316 48-49 -1.545355921358059 2316 50-51 -1.5930383803689665 2316 52-53 -1.8187819127267701 2316 54-55 -1.4975139390989156 2316 56-57 -1.600083656071952 2316 58-59 -1.8589291584929128 2316 60-61 -1.6915927235235415 2316 62-63 -1.7153679931442731 2316 64-65 -1.5857976535865888 2316 66-67 -1.3556271779231253 2316 68-69 -1.6191972519760434 2316 70-71 -1.6720266636343304 2316 72-73 -1.5230848617070727 2316 74-75 -1.3117329853203792 2316 76-77 -1.5843393660208775 2316 78-79 -1.3427666056786087 2316 80-81 -1.5418118498030537 2316 82-83 -1.6499259483258832 2316 84-85 -1.4928901314779601 2316 86-87 -1.334043576233185 2316 88-89 -1.2921432478320405 2316 90-91 -1.4080122879379111 2316 92-93 -1.4720422728006177 2316 94-95 -1.2822034034501542 2316 96-97 -1.2548535044952835 2316 98-99 -1.1167387155563517 2316 100-101 -1.0719587355753788 2316 102-103 -1.0708060250920148 2316 104-105 -1.3199098590678986 2316 106-107 -1.3413872760088523 2316 108-109 -1.3021313307698215 2316 110-111 -1.3781396375112962 2316 112-113 -1.111739981886398 2316 114-115 -1.0390503791343022 2316 116-117 -1.1461914968731506 2316 118-119 -1.1506596933918338 2316 120-121 -1.4060417858179228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34162.0 3 5181.0 4 4662.0 5 5262.0 6 6207.0 7 6271.0 8 7953.0 9 9378.0 10 11484.0 11 13967.0 12 15354.0 13 18041.0 14 22311.0 15 22301.0 16 17483.0 17 17683.0 18 18874.0 19 20074.0 20 22269.0 21 24998.0 22 27940.0 23 30652.0 24 34307.0 25 39038.0 26 44765.0 27 51680.0 28 60349.0 29 70881.0 30 83444.0 31 100445.0 32 121913.0 33 151041.0 34 191417.0 35 248833.0 36 332088.0 37 367340.0 38 132690.0 39 771.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.57481908793296 18.67349053919894 13.91753615513918 21.834154217728923 2 13.916146185116885 21.00413939883011 39.2791762689083 25.800538147144707 3 22.10882188394182 24.450247498767883 30.5319589819434 22.908971635346898 4 11.227823184019625 15.423430182809886 42.17015608600537 31.17859054716512 5 11.176231543394994 38.71193068334439 36.55864253477594 13.553195238484673 6 27.785830040758785 37.175696162401074 18.187406092063927 16.851067704776217 7 25.311362644912343 32.987138338205014 23.551614188079938 18.149884828802705 8 22.95583568294749 31.664664753258904 21.04272293939892 24.336776624394684 9 22.24858664675146 19.33059274437925 25.283787508545025 33.13703310032427 10-11 24.204084178444276 24.530672638634158 28.62795760734707 22.637285575574495 12-13 27.30557457882482 22.939428740004004 27.94158861272955 21.813408068441625 14-15 22.6285847220156 24.956075341798954 29.765451386181716 22.64988855000373 16-17 19.775035603836212 28.76244079288062 29.896684064087538 21.565839539195625 18-19 18.87075868838524 30.81389416411713 30.01232885491754 20.303018292580084 20-21 20.688721070959748 27.428702852855608 28.061867349350173 23.820708726834475 22-23 19.5191121759664 27.070109873619625 29.55459579946062 23.85618215095335 24-25 22.37336606902947 26.743138938794438 28.79661543811215 22.08687955406395 26-27 21.45511807439257 25.145684409560598 32.24974747192687 21.14945004411997 28-29 19.448236378338223 29.081481656361696 30.56272486355362 20.907557101746463 30-31 19.9742183283864 27.264128271721532 29.517526477334055 23.244126922558014 32-33 20.625657413516528 27.111870437629243 30.36142724342692 21.901044905427312 34-35 22.755563538528772 25.913679745180122 29.566901918422307 21.7638547978688 36-37 21.75826821740305 25.41419888166671 31.97171971626947 20.855813184660775 38-39 21.348862712393576 27.25836920438881 31.022379364581116 20.370388718636498 40-41 20.286146057907228 28.425262721151483 30.65799720950354 20.630594011437747 42-43 19.460789273969137 28.704802259887007 28.224260055653932 23.610148410489924 44-45 20.28482570155165 27.142858648421637 28.313715075232302 24.25860057479441 46-47 22.204101074693178 27.820516333270806 28.78293474068469 21.19244785135132 48-49 21.784646177394716 27.05525223691496 29.199583759189053 21.96051782650127 50-51 19.171089767404005 27.991111770810363 31.676703419130313 21.16109504265532 52-53 20.28776004861555 29.228287151300304 28.413938734372636 22.07001406571151 54-55 19.23716979525134 27.42376420132339 29.84411325473715 23.49495274868812 56-57 21.763701526330863 26.32371357264845 28.669518809506638 23.243066091514052 58-59 23.403932359354474 26.084563245344388 28.4539591230775 22.057545272223642 60-61 21.596493303382687 26.61027409984124 31.51482909007945 20.27840350669662 62-63 20.470953615930238 27.710255883757966 30.367101635465254 21.451688864846542 64-65 19.484671510075163 30.137823400383052 29.06569967129296 21.311805418248827 66-67 20.21241253471313 27.520730911940895 27.865532911653368 24.401323641692603 68-69 19.48410047019369 28.205928855504993 29.094217741362478 23.215752932938837 70-71 21.946847086006542 27.26283430899757 27.96584813206142 22.824470472934465 72-73 20.988150768830447 25.871338010215645 30.842941649184063 22.29756957176984 74-75 19.612046969194708 29.426220727548476 29.384299008977322 21.577433294279498 76-77 20.224756304860406 27.124945693207746 28.94916884245336 23.701129159478487 78-79 20.918977724775853 27.089276624273328 29.571066236678817 22.420679414271998 80-81 22.226102405901443 26.80362425567292 28.337433902067446 22.63283943635819 82-83 20.889514967481443 26.085669577200743 31.195219148722053 21.829596306595757 84-85 20.89141877149272 27.32062318288588 29.800102156980103 21.9878558886413 86-87 20.56138871170948 28.515105349998155 29.114511784334297 21.808994153958068 88-89 20.215232336173536 27.42440015325241 27.98174297412147 24.378624536452588 90-91 20.20465115102038 25.704065629070737 29.067279245150125 25.024003974758763 92-93 21.632528010650763 26.789562032914215 29.655581329198867 21.922328627236155 94-95 20.729698650308613 26.006997462713443 30.109773381557382 23.153530505420566 96-97 18.072715970294844 26.971378276809222 32.06000326420996 22.89590248868597 98-99 19.51096039248716 28.12225589415216 29.14494320946718 23.221840503893507 100-101 18.47130426382871 26.72306974238766 30.492882932684346 24.31274306109928 102-103 20.276984899956123 25.630581231395894 29.890614137611916 24.20181973103607 104-105 21.589288782675336 26.232130489461987 28.870276920092806 23.30830380776987 106-107 20.62810296510005 26.328134421692063 31.169771986091632 21.873990627116257 108-109 19.918892621800744 26.63406488801089 30.420158145683356 23.02688434450501 110-111 19.285740790090642 28.154062952230923 28.754570800176758 23.805625457501677 112-113 21.026316183433345 25.80155781415156 27.16589449278687 26.006231509628225 114-115 19.839452443004983 25.6740087548392 29.11312972849877 25.373409073657044 116-117 21.895144510548043 25.020337631039837 28.231352707145916 24.853165151266207 118-119 21.180868620720563 24.895933184643855 29.913032474414337 24.01016572022124 120-121 20.07066316724579 28.43745459935785 28.3474809377925 23.14440129560386 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5855.0 1 6417.5 2 4129.0 3 1264.5 4 1241.5 5 1243.5 6 1304.0 7 1421.5 8 1569.0 9 1699.0 10 1614.5 11 1552.0 12 1700.5 13 1870.5 14 2044.5 15 2224.0 16 2404.5 17 2634.0 18 2930.5 19 3279.5 20 3672.5 21 4162.5 22 4943.0 23 6157.0 24 7677.0 25 9543.0 26 12145.5 27 15247.5 28 19365.5 29 24388.0 30 29445.5 31 34435.5 32 39534.5 33 44695.0 34 49542.5 35 54860.0 36 60436.5 37 65990.5 38 71172.5 39 74048.0 40 76207.5 41 78358.5 42 81228.5 43 85981.0 44 87864.0 45 90074.5 46 99887.5 47 138425.5 48 224253.0 49 217202.5 50 125453.0 51 83106.5 52 63541.5 53 51830.0 54 44133.5 55 37371.5 56 30755.0 57 24107.0 58 18882.0 59 15375.0 60 12284.5 61 9259.5 62 6867.0 63 4899.0 64 3314.0 65 2226.0 66 1421.0 67 851.0 68 488.5 69 285.0 70 180.5 71 125.0 72 85.5 73 58.0 74 35.0 75 29.0 76 27.0 77 17.5 78 10.5 79 9.0 80 10.5 81 9.0 82 7.0 83 7.0 84 4.5 85 3.0 86 3.0 87 2.0 88 1.0 89 2.5 90 3.0 91 2.5 92 3.5 93 3.0 94 1.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4306229890925833 2 0.9052399635848455 3 0.9015215735558129 4 0.8815091148602324 5 0.8908677594276856 6 0.8984716581387411 7 0.8754510636893365 8 0.8681396226210136 9 0.868306741274004 10-11 0.8625411477458409 12-13 0.8641705546124957 14-15 0.8646719105714663 16-17 0.8866689032713059 18-19 0.9123425063369304 20-21 0.9063053449976581 22-23 0.8835145386961152 24-25 0.8761822077961686 26-27 0.8773938180303479 28-29 0.8988894547712166 30-31 0.9053235229113407 32-33 0.8992654717404448 34-35 0.9105668706489092 36-37 0.9205522101650756 38-39 0.9067649212933814 40-41 0.914473269162556 42-43 0.9069947094412429 44-45 0.8922673781464786 46-47 0.885895979501226 48-49 0.8909304289225569 50-51 0.9215758119146408 52-53 0.8952755139003029 54-55 0.9075796247267087 56-57 0.9467271691896709 58-59 0.9119873791993262 60-61 0.9306211090077371 62-63 0.9094597095728488 64-65 0.8977614038635326 66-67 0.9011037769233373 68-69 0.9208028881445609 70-71 0.928281447865874 72-73 0.9334621261085712 74-75 0.9368253889999996 76-77 0.9491921693212769 78-79 0.9761609419475757 80-81 0.9993277652183467 82-83 0.9840990779646117 84-85 0.9462258132307002 86-87 0.9294512784368056 88-89 0.9299108547325287 90-91 0.9420269570743206 92-93 0.9284903461821117 94-95 0.922411405179592 96-97 0.9332741176239572 98-99 0.9645879752280021 100-101 0.9540177204263698 102-103 0.9285321258453593 104-105 0.9181707693599648 106-107 0.9186094558240642 108-109 0.9175440744112515 110-111 0.9163533540086961 112-113 0.9264013630197339 114-115 0.9295557275949243 116-117 0.9569005171904513 118-119 0.9524300932229626 120-121 0.9299944140590238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 2393509.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.052937750759476 #Duplication Level Percentage of deduplicated Percentage of total 1 82.24014782837989 43.63081443352294 2 11.26607041707639 11.953962650656532 3 2.800398672620483 4.457081293675318 4 1.1183009415616731 2.373166009571486 5 0.6041421077758439 1.6025756818222234 6 0.35026078402428407 1.114941814282353 7 0.24798170981848477 0.9209310750028881 8 0.1865659865510825 0.7918298936722887 9 0.1418441610156233 0.6772724500203509 >10 0.9038151573421194 9.219103686790625 >50 0.08506726677635497 3.1538519031767485 >100 0.048201954610876944 4.60258093993287 >500 0.0028812049787382303 1.115384574017767 >1k 0.0027211380354749937 3.302971792334769 >5k 0.0010404351312110272 3.6391709265641907 >10k+ 5.602343014213223E-4 7.444360874956753 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 44138 1.8440707764207278 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 41182 1.7205700918609457 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 27086 1.131643958723364 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 24461 1.0219723426985234 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 15938 0.6658842728395841 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 11286 0.4715252794119429 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11235 0.4693945165863174 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8702 0.36356662958025227 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7894 0.32980866167622513 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7492 0.3130132370507067 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6833 0.2854804389705658 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6707 0.28021620140137343 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6649 0.2777929809330151 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6588 0.275244421474914 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6311 0.2636714547553404 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6046 0.25259984399473745 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5995 0.250469081169112 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5758 0.24056730097944062 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5692 0.23780984320510182 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5041 0.21061128243094135 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4877 0.20375941765834177 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4775 0.19949789200709084 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4296 0.17948543331151043 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4072 0.17012678874405737 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3597 0.15028144870146717 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3581 0.14961297408950625 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3326 0.1389591599613789 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3221 0.13457229532038525 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2881 0.12036720981621545 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2866 0.11974051486750206 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2862 0.11957339621451184 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.355932649511658E-5 0.0 9 0.0 0.0 0.0 1.2533898974267487E-4 0.0 10-11 0.0 0.0 0.0 1.880084846140123E-4 0.0 12-13 0.0 0.0 0.0 2.2978814786157062E-4 0.0 14-15 0.0 4.177966324755829E-5 0.0 3.760169692280246E-4 0.0 16-17 0.0 1.2533898974267487E-4 0.0 5.64025453842037E-4 0.0 18-19 0.0 1.6711865299023316E-4 0.0 6.266949487133744E-4 0.0 20-21 0.0 2.0889831623779146E-4 0.0 9.191525914462824E-4 0.0 22-23 0.0 2.0889831623779146E-4 0.0 0.0018174153512687857 0.0 24-25 0.0 2.0889831623779146E-4 0.0 0.0027992374375864055 0.0 26-27 0.0 2.0889831623779146E-4 0.0 0.00415707649313205 0.0 28-29 0.0 2.0889831623779146E-4 0.0 0.009170636082839044 0.0 30-31 0.0 2.0889831623779146E-4 0.0 0.02274902663829549 0.0 32-33 0.0 2.0889831623779146E-4 0.0 0.05143076545774426 0.0 34-35 0.0 2.5067797948534975E-4 0.0 0.0780444109464389 0.0 36-37 0.0 2.5067797948534975E-4 0.0 0.09763907300954372 0.0 38-39 0.0 2.5067797948534975E-4 0.0 0.11781865035811438 0.0 40-41 0.0 2.9245764273290806E-4 0.0 0.14959208425788245 0.0 42-43 0.0 2.9245764273290806E-4 0.0 0.19099573053621274 0.0 44-45 0.0 2.9245764273290806E-4 0.0 0.22364653736417955 0.0 46-47 0.0 2.9245764273290806E-4 0.0 0.26287764115363677 0.0 48-49 0.0 3.342373059804663E-4 0.0 0.29116247317223376 0.0 50-51 0.0 3.760169692280246E-4 0.0 0.3374961197137759 0.0 52-53 0.0 3.760169692280246E-4 0.0 0.5134302816492439 0.0 54-55 0.0 3.760169692280246E-4 0.0 0.7207409706836281 0.0 56-57 0.0 3.760169692280246E-4 0.0 0.8628753850518214 0.0 58-59 0.0 3.760169692280246E-4 0.0 1.1065134912799577 0.0 60-61 0.0 3.760169692280246E-4 0.0 1.3798569380771077 0.0 62-63 0.0 3.760169692280246E-4 0.0 1.4818410960643975 0.0 64-65 0.0 3.760169692280246E-4 0.0 1.5771822875953254 0.0 66-67 0.0 3.760169692280246E-4 0.0 2.074924305695111 0.0 68-69 0.0 3.760169692280246E-4 0.0 2.44770335102145 0.0 70-71 0.0 3.760169692280246E-4 0.0 2.533330770847321 0.0 72-73 0.0 3.760169692280246E-4 0.0 2.7163883653664973 0.0 74-75 0.0 3.760169692280246E-4 0.0 2.9950169395644637 0.0 76-77 0.0 3.760169692280246E-4 0.0 3.210015086636399 0.0 78-79 0.0 3.760169692280246E-4 0.0 3.4015539527948295 0.0 80-81 0.0 3.760169692280246E-4 0.0 3.7593132091836714 0.0 82-83 0.0 3.760169692280246E-4 0.0 4.15442348451583 0.0 84-85 0.0 3.760169692280246E-4 0.0 4.581724990380232 0.0 86-87 0.0 3.9690680085180375E-4 0.0 5.497326310450473 0.0 88-89 0.0 4.5957629572314124E-4 0.0 6.7173133671107985 0.0 90-91 0.0 5.013559589706995E-4 0.0 8.079142380496585 0.0 92-93 0.0 5.013559589706995E-4 0.0 9.432887029043968 0.0 94-95 0.0 5.013559589706995E-4 0.0 10.515627891936067 0.0 96-97 0.0 5.013559589706995E-4 0.0 11.498264681687012 0.0 98-99 0.0 5.222457905944786E-4 0.0 12.610564656326758 0.0 100-101 0.0 5.431356222182577E-4 0.0 13.618060345710001 0.0 102-103 0.0 5.431356222182577E-4 0.0 14.341558774167968 0.0 104-105 0.0 5.431356222182577E-4 0.0 15.003682877315272 0.0 106-107 0.0 5.431356222182577E-4 0.0 16.06375827289557 0.0 108-109 0.0 5.431356222182577E-4 0.0 17.08059171701464 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2125 0.0 45.49396 2 TATAACG 345 0.0 43.366833 2 ACCTGGG 2395 0.0 40.55151 3 GTACCCG 220 0.0 36.689724 1 TAACGCA 490 0.0 30.493849 4 GTACCTG 3505 0.0 29.444492 1 ATAACGC 515 0.0 29.012953 3 GTATAAC 820 0.0 27.421412 1 GTACCGG 250 1.0113581E-9 25.368322 1 GTACATG 8415 0.0 24.048723 1 TCACGCA 335 1.8189894E-12 24.01697 4 GAGTACG 1055 0.0 23.499306 1 GTACACG 395 0.0 23.354036 1 AGTACGC 850 0.0 23.017778 2 ACGTGCG 175 2.3043196E-5 22.971193 9 CCTGGGG 2030 0.0 22.647953 4 GTACGCA 825 0.0 22.290606 3 TACATGG 7985 0.0 21.835901 2 GTACGGG 185 3.4330373E-5 21.81551 1 TACCGGG 300 8.516508E-9 21.09963 2 >>END_MODULE