##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003802_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2393509 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.602528338101088 31.0 31.0 34.0 30.0 34.0 2 31.802013278412574 31.0 31.0 34.0 30.0 34.0 3 31.8548666414039 31.0 31.0 34.0 30.0 34.0 4 35.5544996906216 37.0 35.0 37.0 33.0 37.0 5 35.4444478796612 37.0 35.0 37.0 33.0 37.0 6 35.52014678031292 37.0 35.0 37.0 33.0 37.0 7 35.45068265880764 37.0 35.0 37.0 33.0 37.0 8 35.46472647481167 37.0 35.0 37.0 33.0 37.0 9 37.00933900812573 39.0 37.0 39.0 33.0 39.0 10-11 36.91924053763742 39.0 37.0 39.0 33.0 39.0 12-13 36.87697936377093 39.0 37.0 39.0 33.0 39.0 14-15 37.8776144146523 40.0 37.0 41.0 33.0 41.0 16-17 37.945484642004686 40.0 37.0 41.0 33.0 41.0 18-19 37.999950908895684 40.0 37.0 41.0 33.0 41.0 20-21 37.93569483131252 40.0 37.0 41.0 32.5 41.0 22-23 37.834409855989676 39.5 37.0 41.0 32.0 41.0 24-25 37.702822090913386 39.5 37.0 41.0 32.0 41.0 26-27 37.43090834419257 39.0 36.5 41.0 31.5 41.0 28-29 37.29557691239097 39.0 36.0 40.5 31.0 41.0 30-31 37.12886373103255 39.0 36.0 40.0 31.0 41.0 32-33 36.887361192291316 39.0 35.5 40.0 31.0 41.0 34-35 36.84314431238821 39.0 35.0 40.0 30.0 41.0 36-37 36.64012293248114 38.5 35.0 40.0 30.0 41.0 38-39 36.38779026943287 38.0 35.0 40.0 30.0 41.0 40-41 36.60880176343603 39.0 35.0 40.0 30.0 41.0 42-43 36.483717420740845 39.0 35.0 40.5 30.0 41.0 44-45 36.33072112952155 39.0 35.0 40.0 30.0 41.0 46-47 36.05713306279608 39.0 35.0 40.0 29.5 41.0 48-49 35.777897847887765 39.0 35.0 40.0 28.0 41.0 50-51 35.48669672852703 38.0 34.5 40.0 27.5 41.0 52-53 35.35497192615528 38.0 34.0 40.0 27.0 41.0 54-55 35.16800772422414 38.0 34.0 40.0 27.0 41.0 56-57 34.87528645181614 38.0 34.0 40.0 26.0 41.0 58-59 34.603446446200955 38.0 34.0 40.0 26.0 41.0 60-61 34.06974070287599 37.0 33.0 40.0 23.5 41.0 62-63 33.89346311210862 37.0 33.0 40.0 24.0 41.0 64-65 33.44352747367986 36.5 32.0 39.0 22.5 41.0 66-67 33.06608059547719 36.0 32.0 39.0 22.0 40.5 68-69 32.739627049658054 35.5 32.0 39.0 22.0 40.0 70-71 32.23111987462759 35.0 31.0 38.0 21.5 40.0 72-73 31.820392152275176 35.0 31.0 37.0 20.5 39.5 74-75 31.46421947859816 35.0 31.0 37.0 20.0 39.0 76-77 30.51277601212279 34.0 29.5 36.0 19.0 38.5 78-79 30.888926049578256 34.0 30.0 36.0 18.5 38.5 80-81 30.654526889182367 34.0 30.0 36.0 18.0 37.5 82-83 30.330261762124145 34.0 30.0 35.0 15.0 37.0 84-85 29.99446127004327 34.0 30.0 35.0 11.5 36.5 86-87 29.732775811580403 34.0 30.0 35.0 7.5 36.0 88-89 29.408401639601102 34.0 29.0 35.0 6.5 36.0 90-91 29.063940641125644 34.0 29.0 35.0 2.0 35.5 92-93 28.815342453276756 34.0 29.0 35.0 2.0 35.0 94-95 28.445359720811577 34.0 29.0 35.0 2.0 35.0 96-97 27.97912312007183 34.0 29.0 35.0 2.0 35.0 98-99 27.665167751614888 34.0 29.0 35.0 2.0 35.0 100-101 26.633635595270377 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04017144424796726 1101 2 0.09711812084988836 1101 3 0.052036195514226335 1101 4 0.09543137562092596 1101 5 0.17564661501008771 1101 6 0.17679432784252924 1101 7 0.11764074320024775 1101 8 0.22434277793409052 1101 9 0.19855359775144876 1101 10-11 0.11426723392975191 1101 12-13 0.08535501205078333 1101 14-15 0.08886955660224771 1101 16-17 0.1381931522072506 1101 18-19 -0.06368922248069708 1101 20-21 -0.0049288685840664925 1101 22-23 -0.019588571770448482 1101 24-25 0.048092647045343995 1101 26-27 0.22978721631125154 1101 28-29 0.20780989728907429 1101 30-31 0.1394387823463603 1101 32-33 0.25686952249449746 1101 34-35 0.052891264434002494 1101 36-37 0.10092117367408804 1101 38-39 0.20435098373564387 1101 40-41 0.13126641028511443 1101 42-43 0.08428328388665562 1101 44-45 0.2794945351220903 1101 46-47 0.36507378928953216 1101 48-49 0.27476116700690767 1101 50-51 0.32866458257174713 1101 52-53 0.4384082684584385 1101 54-55 0.3942236949945297 1101 56-57 0.2747933780304592 1101 58-59 0.14415520065815457 1101 60-61 0.35368286274898253 1101 62-63 0.18704918401441972 1101 64-65 -0.025760873060150402 1101 66-67 0.08052741778703165 1101 68-69 0.10138564619980883 1101 70-71 0.21216056928843585 1101 72-73 0.2061429544167943 1101 74-75 0.12469812603044517 1101 76-77 0.00958962199751312 1101 78-79 0.08754424020438023 1101 80-81 0.17228520864576424 1101 82-83 0.20734874636592693 1101 84-85 0.12919402591619544 1101 86-87 -0.13188601995857852 1101 88-89 -0.03463392411977395 1101 90-91 0.09213735888867447 1101 92-93 0.2622982377870322 1101 94-95 0.2154768875336579 1101 96-97 0.2614265528207085 1101 98-99 -0.034593974331162514 1101 100-101 0.08899473870718566 1102 1 0.001707012217675441 1102 2 0.0014845606162481317 1102 3 -0.07724233540756131 1102 4 -0.009731819430982114 1102 5 -0.13193618223398573 1102 6 0.03824558167038816 1102 7 -0.06124279332838256 1102 8 -0.08782532416526578 1102 9 -0.03543272501679695 1102 10-11 -0.01777343095002948 1102 12-13 -0.04373354495318438 1102 14-15 -0.023315105613669118 1102 16-17 -0.02086543187606793 1102 18-19 -0.10657096544186828 1102 20-21 -0.024765228367733982 1102 22-23 -0.22094039144680977 1102 24-25 -0.03612856673107245 1102 26-27 -0.07460052457278721 1102 28-29 0.05885904967374955 1102 30-31 -0.019228446562976842 1102 32-33 -0.011912799224937487 1102 34-35 -0.14663577062403732 1102 36-37 -0.09798758364497218 1102 38-39 -0.12434696374474896 1102 40-41 -0.1504659114565854 1102 42-43 0.03297107271489352 1102 44-45 0.14145683770836115 1102 46-47 -0.035943455935075974 1102 48-49 -0.11084381812236899 1102 50-51 0.009900980016325889 1102 52-53 0.03998944797499604 1102 54-55 -0.09601373790250278 1102 56-57 0.11362744121086621 1102 58-59 -0.12061179360501484 1102 60-61 -0.03959909415821272 1102 62-63 -0.3470248695326319 1102 64-65 -0.24667922723385516 1102 66-67 -0.22493049837159873 1102 68-69 -0.07234260807455684 1102 70-71 -0.152145190115327 1102 72-73 -0.13159611557240325 1102 74-75 -0.12490239454483287 1102 76-77 -0.0650529332284755 1102 78-79 -0.022577697008738795 1102 80-81 -0.02961928266262248 1102 82-83 -0.180662853283458 1102 84-85 -0.07980794438154248 1102 86-87 -0.22565055735928752 1102 88-89 -0.14678870467374594 1102 90-91 -0.08173262460907793 1102 92-93 0.026116809245493755 1102 94-95 -0.2189108288663313 1102 96-97 -0.22064951375985942 1102 98-99 -0.3865522081146011 1102 100-101 -0.4274680537825333 1103 1 0.036401172654247915 1103 2 -0.023661812671040394 1103 3 -0.03772305451247604 1103 4 0.08505900914880016 1103 5 0.03859962083781454 1103 6 0.05028389937951516 1103 7 -0.02849073445965189 1103 8 -0.019748562186457264 1103 9 -0.04406520828518978 1103 10-11 0.022483366405460004 1103 12-13 -0.11272711748593167 1103 14-15 0.08155988572296025 1103 16-17 -0.0484209753782352 1103 18-19 -0.09801720584059126 1103 20-21 -0.018328344408679698 1103 22-23 -0.11516327961852113 1103 24-25 -0.059120397549769166 1103 26-27 -0.11213175037184442 1103 28-29 -0.16997859726454578 1103 30-31 -0.03437616078927874 1103 32-33 0.026499453643012316 1103 34-35 -0.004591908358058561 1103 36-37 -0.01516312913595641 1103 38-39 0.16502240318137495 1103 40-41 0.16963129908891972 1103 42-43 0.1658747028929568 1103 44-45 0.19025119792978273 1103 46-47 0.3479116382771892 1103 48-49 0.4907609393484691 1103 50-51 0.41484686032018914 1103 52-53 0.4271667562734578 1103 54-55 0.4815467776207569 1103 56-57 0.4406444244295358 1103 58-59 0.31245877594754035 1103 60-61 0.36291237915710184 1103 62-63 0.2736743322319839 1103 64-65 0.2577552882351526 1103 66-67 0.15046010661790632 1103 68-69 0.20766994251199122 1103 70-71 0.23262972799698645 1103 72-73 0.37959432187505726 1103 74-75 0.41589793511523254 1103 76-77 0.39014426014095704 1103 78-79 0.33438968653296897 1103 80-81 0.45528422999493756 1103 82-83 0.30618050093235993 1103 84-85 0.18598989926798382 1103 86-87 0.31797892762973134 1103 88-89 0.2229379396338942 1103 90-91 0.16075435020236029 1103 92-93 0.03111745711490599 1103 94-95 -0.09019687978585367 1103 96-97 0.042451385121282215 1103 98-99 0.12283587169085664 1103 100-101 0.24489572037320073 1104 1 0.00977273794355682 1104 2 -0.008933517072346575 1104 3 0.027991355773909987 1104 4 0.010727284936635328 1104 5 -0.013033548585880794 1104 6 0.0025897386772300024 1104 7 -0.007744107687202018 1104 8 -0.06141008538791226 1104 9 -0.03224232333939625 1104 10-11 -0.019584728986401956 1104 12-13 -0.05271166665269078 1104 14-15 0.09117748441277485 1104 16-17 -0.06213733677513744 1104 18-19 -0.006697535730872062 1104 20-21 -0.04787595109640819 1104 22-23 -0.11327552569824206 1104 24-25 -0.04310582689627296 1104 26-27 0.12431334535110494 1104 28-29 -0.1128044511138313 1104 30-31 -0.0961833769006546 1104 32-33 0.058804570811830104 1104 34-35 0.0034576487589319527 1104 36-37 -0.05441178808810321 1104 38-39 7.980309225601445E-4 1104 40-41 -0.05941081737548615 1104 42-43 0.11524876702072362 1104 44-45 0.2561145983938573 1104 46-47 0.19530999863585663 1104 48-49 0.31423850770887185 1104 50-51 0.4038527885896315 1104 52-53 0.20289287049789806 1104 54-55 -0.05357396096722766 1104 56-57 0.03113507694793327 1104 58-59 0.13802820182781517 1104 60-61 0.32541781215512344 1104 62-63 0.13352566905606267 1104 64-65 0.20455462224156662 1104 66-67 0.3285864818987463 1104 68-69 0.24349904866630112 1104 70-71 0.29128716776394725 1104 72-73 0.32957470176934933 1104 74-75 0.3982809877039273 1104 76-77 0.12306509437695468 1104 78-79 0.3037091549916049 1104 80-81 0.2698187089244435 1104 82-83 0.4452827584707748 1104 84-85 0.3608809327434237 1104 86-87 0.21251411506305118 1104 88-89 0.25098862763197616 1104 90-91 0.25138908787200975 1104 92-93 0.2574861298011335 1104 94-95 0.2467336091771557 1104 96-97 0.1386542134140214 1104 98-99 0.14791915495204577 1104 100-101 0.24132111424001224 1105 1 0.03829757988886584 1105 2 0.03149730895773217 1105 3 0.04737330420242003 1105 4 0.045780619978302184 1105 5 -0.027672917852399337 1105 6 0.048530587418866844 1105 7 0.009531859431220369 1105 8 0.05120695097751593 1105 9 -0.01099203669058113 1105 10-11 0.09339734087511431 1105 12-13 0.001261061517730866 1105 14-15 0.19020291924169186 1105 16-17 0.0054611867692386795 1105 18-19 0.005542470732500249 1105 20-21 -0.012400946465142226 1105 22-23 0.21805459825645812 1105 24-25 0.07114565857705912 1105 26-27 0.12099311557504677 1105 28-29 0.09349668152642465 1105 30-31 0.18746486889019565 1105 32-33 0.22429858199379282 1105 34-35 0.22889598682417756 1105 36-37 0.19686631879742578 1105 38-39 0.22870975043190356 1105 40-41 0.16914625981036835 1105 42-43 0.3120191223510034 1105 44-45 0.3469451822107814 1105 46-47 0.3145120009747515 1105 48-49 0.22000722842682308 1105 50-51 0.2662837990542499 1105 52-53 0.3300707690376825 1105 54-55 0.3340985485748149 1105 56-57 0.4426272258306767 1105 58-59 0.3100876746225012 1105 60-61 0.3401449102408449 1105 62-63 0.3053208944864849 1105 64-65 0.2593886433960435 1105 66-67 0.24130609977649442 1105 68-69 0.3592428217563324 1105 70-71 0.4499325368656102 1105 72-73 0.3072788508120503 1105 74-75 0.37496968586537704 1105 76-77 0.2743023971605254 1105 78-79 0.3395387472688576 1105 80-81 0.4366691300635779 1105 82-83 0.5023108161873608 1105 84-85 0.37592834302948575 1105 86-87 0.30089775849396005 1105 88-89 0.3708677332387005 1105 90-91 0.33638966320984665 1105 92-93 0.35419633988513155 1105 94-95 0.5651417269084185 1105 96-97 0.5870342497623149 1105 98-99 0.5002806923526748 1105 100-101 0.5399092923123767 1106 1 -0.03128085834214289 1106 2 -0.0037139530014869138 1106 3 0.03220020750439545 1106 4 -0.0019079162115502868 1106 5 0.018158469466875715 1106 6 0.01610855660202759 1106 7 0.051953629665923984 1106 8 0.07295413418479768 1106 9 0.06354968952578588 1106 10-11 -0.046975552167943135 1106 12-13 -0.15447284803622097 1106 14-15 -0.12518455637884784 1106 16-17 -0.02772815822748953 1106 18-19 -0.0097927882360338 1106 20-21 -0.006448849480484853 1106 22-23 -0.0749544840333769 1106 24-25 -0.047364392877888406 1106 26-27 -0.21495529317009243 1106 28-29 -0.05632171276641884 1106 30-31 -0.03268444467631326 1106 32-33 0.012873358043485439 1106 34-35 -0.018484060200869123 1106 36-37 -0.05012425815147736 1106 38-39 0.07151417551754236 1106 40-41 -0.07346914299950669 1106 42-43 -0.12450577426803733 1106 44-45 -0.016567204461047425 1106 46-47 -0.007823296078868225 1106 48-49 -0.06711951271288541 1106 50-51 0.28141480171419175 1106 52-53 0.323705849808114 1106 54-55 0.047639499058192314 1106 56-57 0.05898456813338271 1106 58-59 0.1212312694132649 1106 60-61 0.3471636901952593 1106 62-63 0.133446126254249 1106 64-65 0.25157609015209914 1106 66-67 0.035412032430045315 1106 68-69 0.1584180792816383 1106 70-71 0.07100591479190399 1106 72-73 0.1443303238027056 1106 74-75 0.13113475863794832 1106 76-77 0.025605708758639878 1106 78-79 0.17088607720393156 1106 80-81 0.23665108976154414 1106 82-83 0.1465377190884425 1106 84-85 0.13148986747509994 1106 86-87 0.23911383320486124 1106 88-89 0.16353996299999096 1106 90-91 0.07118659956566731 1106 92-93 0.06527138152816292 1106 94-95 0.16931555123195352 1106 96-97 0.3121517622852892 1106 98-99 0.16506451942235145 1106 100-101 0.17516355032477549 1107 1 -0.027094053801285156 1107 2 -0.12634283539630076 1107 3 -0.1316396636132069 1107 4 -0.17515451618906752 1107 5 -0.07542410518375675 1107 6 -0.05702564593815396 1107 7 -0.06626505828132423 1107 8 -0.13898200533788696 1107 9 -0.053552517210569306 1107 10-11 -0.22936427870497766 1107 12-13 -0.16560253594599317 1107 14-15 -0.17555172134081687 1107 16-17 -0.13859137054624426 1107 18-19 -0.201330900333744 1107 20-21 -0.18853808367926916 1107 22-23 -0.17288309292776205 1107 24-25 -0.24866398824710956 1107 26-27 -0.0880459417033066 1107 28-29 -0.23705398207068384 1107 30-31 -0.05563459641285107 1107 32-33 -0.19520965683044267 1107 34-35 -0.1471091117163681 1107 36-37 0.0019632900857544655 1107 38-39 0.19234352810830302 1107 40-41 0.27322285992283213 1107 42-43 0.14510336364361365 1107 44-45 0.16754752522312089 1107 46-47 -0.17449534845606962 1107 48-49 -0.14068644538681951 1107 50-51 -0.05459780553169935 1107 52-53 -0.014422639195664999 1107 54-55 -0.18556270393157348 1107 56-57 -0.22487258409022104 1107 58-59 -0.19794349923534327 1107 60-61 -0.18966192518070812 1107 62-63 -0.25875466240109546 1107 64-65 -0.3070307436120885 1107 66-67 -0.40257361816379245 1107 68-69 -0.4174335654675261 1107 70-71 -0.39722518370572146 1107 72-73 -0.5025742976052605 1107 74-75 -0.33830437814972925 1107 76-77 -0.32894006509771856 1107 78-79 -0.3504965986043942 1107 80-81 -0.4462342929517291 1107 82-83 -0.4714777168262856 1107 84-85 -0.488861093522992 1107 86-87 -0.4989149030732847 1107 88-89 -0.6769233486031752 1107 90-91 -0.5648515312649529 1107 92-93 -0.7084105243577206 1107 94-95 -0.5890582920110852 1107 96-97 -0.45397399171418584 1107 98-99 -0.3703774366677166 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-0.6006912016142536 2316 80-81 -0.6246111639388374 2316 82-83 -0.5630657791399507 2316 84-85 -0.6557026485155113 2316 86-87 -0.5292259117528317 2316 88-89 -0.5442285802443507 2316 90-91 -0.704150082808038 2316 92-93 -0.806646568905272 2316 94-95 -1.0059325988173562 2316 96-97 -0.8401178758376311 2316 98-99 -0.9853665109170322 2316 100-101 -1.3163343666182534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 19.0 5 54.0 6 130.0 7 301.0 8 715.0 9 1456.0 10 2459.0 11 3934.0 12 5915.0 13 8513.0 14 10490.0 15 13634.0 16 17209.0 17 21613.0 18 19545.0 19 14433.0 20 13295.0 21 13865.0 22 14943.0 23 16480.0 24 19654.0 25 23558.0 26 29659.0 27 36634.0 28 46600.0 29 58428.0 30 71544.0 31 87848.0 32 108592.0 33 136679.0 34 174743.0 35 230600.0 36 324817.0 37 424123.0 38 367226.0 39 73722.0 40 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.72591695278988 20.20305761004144 13.984841530794718 20.086183906373964 2 14.671697230265274 24.201941195886974 38.21573764190242 22.91062393194533 3 24.45995897343304 25.749687786822346 28.785093680671537 21.005259559073075 4 12.600662876137086 16.693941823490114 40.44772758322613 30.25766771714667 5 11.960598435184492 40.636237423799116 34.134820466520075 13.268343674496313 6 28.220583608768298 40.10291261243533 16.68135235328205 14.995151425514322 7 27.491185535546347 33.897219521631214 21.542137506063273 17.06945743675917 8 24.495082324737446 32.80530802265628 18.95196550336765 23.747644149238628 9 23.460325405085168 21.551370811640986 22.60154442703161 32.38675935624224 10-11 25.059160176177 26.209148770295577 27.688946990643835 21.04274406288359 12-13 29.17822744765113 23.9312239895484 26.369464246844277 20.521084315956198 14-15 22.71201418041169 26.945560630765105 28.55984633012909 21.78257885869412 16-17 20.087745648752524 29.403378052892222 29.11198579157212 21.396890506783137 18-19 19.68758003416741 32.1898518033565 28.900183788738627 19.22238437373747 20-21 22.267644284456274 27.602103013392977 27.99040628104457 22.13984642110618 22-23 20.627434448752858 28.24881794887757 28.265655153166335 22.85809244920324 24-25 23.10712013199031 28.491578682177504 28.411967533859283 19.9893336519729 26-27 22.979945590658073 25.97968779182556 31.28996769784807 19.750398919668296 28-29 20.1396409947854 30.895883473726432 29.637234255916567 19.3272412755716 30-31 21.60724746560993 28.2240926461531 28.233995382065935 21.934664506171035 32-33 21.629184728678123 28.73847600146237 28.89935760171306 20.732981668146447 34-35 23.55447515160277 26.876250734589497 29.76275657881955 19.80651753498819 36-37 23.120899959695628 26.35435965250066 30.656647892085402 19.86809249571831 38-39 21.206657625982682 28.395526511899792 31.302529128210505 19.095286733907017 40-41 21.253452162157135 29.067147909753547 28.993022657716207 20.68637727037311 42-43 19.959649201235507 30.32986297523845 27.621976771342826 22.088511052183218 44-45 22.097723467929303 27.051767927340155 27.83707936757288 23.013429237157663 46-47 23.56146292387575 29.310911014293666 27.355371279310535 19.77225478252005 48-49 23.265736623509667 28.57306991534187 28.16906057173798 19.992132889410485 50-51 20.24765730983255 29.132207148583937 30.27757572668413 20.34255981489938 52-53 21.848654005478984 31.07258004879029 26.93697830256749 20.14178764316324 54-55 20.420666895340688 28.974614258814153 27.848526995302713 22.756191850542447 56-57 22.612551052745154 27.698032217099744 28.08207545455248 21.60734127560262 58-59 25.2413405272433 27.03893368138622 26.860137388416828 20.85958840295366 60-61 21.880450767006288 28.5991906149609 30.185808414621075 19.334550203411734 62-63 21.198087746223983 28.24172017598217 29.549594120333595 21.01059795746025 64-65 20.595300494645173 31.686320389964646 27.579436236583682 20.1389428788065 66-67 21.753130881895714 27.904423687052223 27.286438082756913 23.05600734829515 68-69 20.849953185183203 29.514245617142954 27.18658200865437 22.44921918901947 70-71 22.977946744549772 29.044372857088245 27.01600563024506 20.961674768116918 72-73 22.675765998790897 26.71765596034943 29.23185164542937 21.374726395430308 74-75 20.579826522482264 31.608446009603476 27.704387157098637 20.107340310815626 76-77 22.034865964573353 28.295611171714835 26.934262624456395 22.73526023925542 78-79 21.698184548292904 29.115537062948167 27.33522205264321 21.85105633611572 80-81 23.09076339382889 28.285500493208925 27.511636680706026 21.112099432256155 82-83 22.471358996352215 27.529246808764874 28.94486713858189 21.05452705630102 84-85 20.711587343764883 28.921545279982354 28.979180349512095 21.387687026740668 86-87 21.50314268859056 29.08528626792987 26.82327745402951 22.58829358945006 88-89 20.927412430870323 29.756541546323827 26.62640917581676 22.689636846989085 90-91 22.239189407685537 26.161756651009043 27.551682487928808 24.047371453376613 92-93 22.442092342247303 28.79450213055393 27.232193403074735 21.53121212412404 94-95 21.69186752638288 28.470384202568372 28.19735341881752 21.64039485223123 96-97 19.277333692640163 28.660234656606075 30.17853580377669 21.88389584697707 98-99 20.769034885618034 30.276685522647128 26.937278083270332 22.01700150846451 100-101 19.227337629035578 29.14318183479895 27.24157632566005 24.387904210505425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 196.0 1 147.0 2 201.5 3 318.0 4 284.0 5 254.5 6 399.0 7 756.5 8 1140.5 9 1417.0 10 1532.5 11 1558.0 12 1702.5 13 1921.5 14 2184.5 15 2493.5 16 2736.0 17 2960.5 18 3298.0 19 3657.5 20 4009.0 21 4556.5 22 5322.0 23 6373.0 24 7852.5 25 9637.5 26 11972.0 27 15191.5 28 19541.5 29 24790.5 30 29625.5 31 34296.5 32 39512.5 33 44675.0 34 49392.0 35 53949.0 36 59284.0 37 64955.0 38 70684.0 39 73951.5 40 75227.5 41 77095.0 42 80396.5 43 85030.0 44 86450.0 45 88538.5 46 96434.5 47 139031.5 48 237630.5 49 229664.0 50 129387.0 51 84591.5 52 63285.5 53 51512.0 54 43632.5 55 36867.5 56 30543.0 57 24189.0 58 19055.5 59 15328.5 60 12179.5 61 9158.5 62 6740.5 63 4781.5 64 3207.5 65 2060.0 66 1241.5 67 727.0 68 379.5 69 199.5 70 114.5 71 75.0 72 47.5 73 30.0 74 19.0 75 8.0 76 7.0 77 5.5 78 3.0 79 2.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04942534162186146 2 0.005807373191410603 3 0.03814483254502072 4 0.0 5 0.0 6 9.191525914462825E-4 7 0.0 8 0.0 9 0.0 10-11 2.9245764273290806E-4 12-13 0.0 14-15 0.0027783476059626265 16-17 0.0 18-19 0.0 20-21 0.011552076887949868 22-23 0.0 24-25 0.0 26-27 0.007102542752084909 28-29 0.009567542883690848 30-31 0.009651102210185966 32-33 0.005598474875172811 34-35 0.006914534267470898 36-37 0.01957377223148106 38-39 0.010591144633256028 40-41 0.010967161602484052 42-43 0.0 44-45 0.0 46-47 4.177966324755829E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0011907204025554112 58-59 1.2533898974267487E-4 60-61 0.008794619113611022 62-63 0.013035254933238188 64-65 0.04541449395009586 66-67 0.023939747040850903 68-69 0.0015040678769120985 70-71 3.133474743566872E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 4.177966324755829E-5 86-87 7.10254275208491E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 2.5067797948534975E-4 96-97 4.804661273469203E-4 98-99 6.475847803371536E-4 100-101 0.0013160593922980862 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2393509.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.38846007755866 #Duplication Level Percentage of deduplicated Percentage of total 1 80.42739914749 39.72185391937685 2 12.215889265079246 12.066479185604877 3 3.15670726782115 4.677147326199725 4 1.2992398614789382 2.566698241193016 5 0.6748245844045418 1.6664273523109416 6 0.41538279710123155 1.2309070014923296 7 0.2872057394356968 0.9929254437315941 8 0.19695471980839463 0.7781832253074921 9 0.1569486872759839 0.6976308578179653 >10 0.9963824209609022 9.584226418194906 >50 0.10452187700260337 3.578937560732873 >100 0.060284130384951194 5.492793112499919 >500 0.0031183833161278728 1.0058658385671742 >1k 0.003539786466955951 3.869326329637432 >5k 5.899644111593251E-4 2.083826013256765 >10k+ 0.0010113675619874147 9.986772174076203 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 77626 3.24318813925496 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 20333 0.8495058928126027 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 18193 0.760097413462828 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 17793 0.7433855481638046 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 16417 0.6858967315351645 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 16257 0.6792119854155552 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 15235 0.6365131695765506 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14007 0.585207743108549 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 12116 0.5062023999074162 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11516 0.48113460195888125 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10422 0.4354276503660525 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10008 0.41813086978156344 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9655 0.40338264865517526 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7757 0.32408484781130964 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7559 0.31581247448829314 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6955 0.2905775578867679 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 6477 0.2706068788544351 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5943 0.24829653868023893 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5716 0.2388125551230432 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4821 0.20141975651647853 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4457 0.1862119590943673 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4188 0.17497322968077414 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3944 0.16477899184836992 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3931 0.16423585622615164 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3884 0.1622722120535164 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3879 0.1620633137372786 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3807 0.15905517798345442 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3321 0.1387502616451411 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3013 0.12588212536489313 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2890 0.12074322678544347 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2637 0.11017297198381122 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 2.0889831623779144E-5 0.0 6.266949487133744E-5 0.0 12-13 0.0 4.177966324755829E-5 0.0 1.4622882136645403E-4 0.0 14-15 0.0 1.0444915811889572E-4 0.0 3.760169692280246E-4 0.0 16-17 0.0 1.6711865299023316E-4 0.0 6.475847803371535E-4 0.0 18-19 0.0 2.0889831623779146E-4 0.0 8.147034333273867E-4 0.0 20-21 0.0 2.0889831623779146E-4 0.0 0.0012116102341791905 0.0 22-23 0.0 2.0889831623779146E-4 0.0 0.002130762825625473 0.0 24-25 0.0 2.0889831623779146E-4 0.0 0.003133474743566872 0.0 26-27 0.0 2.0889831623779146E-4 0.0 0.004574873125607633 0.0 28-29 0.0 2.0889831623779146E-4 0.0 0.009901780189671316 0.0 30-31 0.0 2.0889831623779146E-4 0.0 0.024253094515207588 0.0 32-33 0.0 2.0889831623779146E-4 0.0 0.05412555373721177 0.0 34-35 0.0 2.0889831623779146E-4 0.0 0.08170013148060024 0.0 36-37 0.0 2.5067797948534975E-4 0.0 0.10215127664028002 0.0 38-39 0.0 2.9245764273290806E-4 0.0 0.12322911674867318 0.0 40-41 0.0 3.342373059804663E-4 0.0 0.15640216936723447 0.0 42-43 0.0 3.342373059804663E-4 0.0 0.19989479880794264 0.0 44-45 0.0 3.342373059804663E-4 0.0 0.23404967351282155 0.0 46-47 0.0 3.342373059804663E-4 0.0 0.27426259938859643 0.0 48-49 0.0 3.342373059804663E-4 0.0 0.3034248043353921 0.0 50-51 0.0 3.760169692280246E-4 0.0 0.351011840774361 0.0 52-53 0.0 4.1779663247558293E-4 0.0 0.5294110028414349 0.0 54-55 0.0 4.1779663247558293E-4 0.0 0.7405654208945944 0.0 56-57 0.0 4.1779663247558293E-4 0.0 0.8854990727003742 0.0 58-59 0.0 4.1779663247558293E-4 0.0 1.1340880690233461 0.0 60-61 0.0 4.5957629572314124E-4 0.0 1.41292554153755 0.0 62-63 0.0 4.5957629572314124E-4 0.0 1.5162466487487618 0.0 64-65 0.0 4.5957629572314124E-4 0.0 1.6122980945548984 0.0 66-67 0.0 4.5957629572314124E-4 0.0 2.1185840537888097 0.0 68-69 0.0 4.5957629572314124E-4 0.0 2.5003248368817497 0.0 70-71 0.0 4.5957629572314124E-4 0.0 2.5887932738084545 0.0 72-73 0.0 5.013559589706995E-4 0.0 2.77820137714126 0.0 74-75 0.0 5.431356222182577E-4 0.0 3.0661677060750554 0.0 76-77 0.0 5.431356222182577E-4 0.0 3.290127590913592 0.0 78-79 0.0 5.431356222182577E-4 0.0 3.4883929828548794 0.0 80-81 0.0 5.431356222182577E-4 0.0 3.860858680706862 0.0 82-83 0.0 5.431356222182577E-4 0.0 4.271448321272241 0.0 84-85 0.0 5.431356222182577E-4 0.0 4.712620675334833 0.0 86-87 0.0 5.431356222182577E-4 0.0 5.6592225055347605 0.0 88-89 0.0 5.431356222182577E-4 0.0 6.917751301540959 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2095 0.0 41.055706 2 ACCTGGG 2190 0.0 38.621365 3 TATAACG 325 0.0 36.554058 2 GTACCTG 2970 0.0 29.774487 1 GTACCCG 245 0.0 29.10804 1 CCTGGGG 1795 0.0 25.664995 4 ATAACGC 430 0.0 25.41623 3 GTATAAC 680 0.0 25.169893 1 GTACCGG 200 2.0367224E-8 23.771564 1 TCACGCA 300 0.0 23.746737 4 TAACGCA 420 0.0 23.746737 4 TACGCAG 745 0.0 23.587362 4 GAGTACG 920 0.0 22.73802 1 GTACACG 335 0.0 22.707167 1 AGTACGC 775 0.0 22.687098 2 GTACGCA 790 0.0 22.254936 3 GGACCGA 170 7.966855E-5 19.556135 6 AGTACTT 8590 0.0 19.185772 12-13 GTCGTAT 150 7.677386E-4 19.017254 1 TACCCGG 275 2.8272552E-8 19.008112 2 >>END_MODULE