##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003801_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2793612 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.721774892146797 31.0 31.0 34.0 30.0 34.0 2 31.925797498006165 31.0 31.0 34.0 30.0 34.0 3 31.985340841892146 31.0 31.0 34.0 30.0 34.0 4 35.65405038351783 37.0 35.0 37.0 33.0 37.0 5 35.56063941592462 37.0 35.0 37.0 33.0 37.0 6 35.635207394584505 37.0 35.0 37.0 33.0 37.0 7 35.585544449264965 37.0 35.0 37.0 33.0 37.0 8 35.59766961195756 37.0 35.0 37.0 33.0 37.0 9 37.15015148846726 39.0 37.0 39.0 33.0 39.0 10-11 37.06296472094192 39.0 37.0 39.0 33.0 39.0 12-13 37.044092737287784 39.0 37.0 39.0 33.0 39.0 14-15 38.03401957752186 40.0 37.0 41.0 33.0 41.0 16-17 38.15144622803739 40.0 37.0 41.0 33.0 41.0 18-19 38.20112116500073 40.0 37.5 41.0 33.0 41.0 20-21 38.12555591112867 40.0 37.5 41.0 33.0 41.0 22-23 38.0261317606024 40.0 37.0 41.0 33.0 41.0 24-25 37.8534193366867 39.5 37.0 41.0 32.5 41.0 26-27 37.58621472845907 39.0 36.5 41.0 32.0 41.0 28-29 37.52689636212903 39.0 36.5 41.0 32.0 41.0 30-31 37.400286976144145 39.0 36.0 41.0 32.0 41.0 32-33 37.15469113105184 39.0 36.0 40.0 31.0 41.0 34-35 37.08167293811739 39.0 36.0 40.0 31.0 41.0 36-37 36.884377465446164 39.0 35.5 40.5 30.5 41.0 38-39 36.66740674796643 38.5 35.0 40.0 30.0 41.0 40-41 37.006157261638336 39.0 36.0 40.5 31.0 41.0 42-43 36.89414492778525 39.0 36.0 41.0 31.0 41.0 44-45 36.74908326567898 39.0 36.0 41.0 30.0 41.0 46-47 36.51982415596726 39.0 35.0 41.0 30.0 41.0 48-49 36.22778628528228 39.0 35.0 41.0 29.5 41.0 50-51 35.9451620339546 39.0 35.0 40.0 28.0 41.0 52-53 35.942385878926636 39.0 35.0 40.0 28.5 41.0 54-55 35.78491895080634 39.0 35.0 40.0 28.0 41.0 56-57 35.43563154081526 38.0 34.5 40.0 27.5 41.0 58-59 35.162511472602496 38.0 34.0 40.0 27.0 41.0 60-61 34.52541673646877 38.0 33.0 40.0 26.0 41.0 62-63 34.48339157334662 37.0 33.5 40.0 26.0 41.0 64-65 34.10596800844212 37.0 33.0 39.5 25.5 41.0 66-67 33.67880399998282 36.0 32.5 39.0 25.0 40.5 68-69 33.28248464711635 36.0 32.0 39.0 24.5 40.0 70-71 32.70090334663511 35.0 31.5 38.5 23.0 40.0 72-73 32.25428173275316 35.0 31.0 37.0 22.0 40.0 74-75 31.945711501811992 35.0 31.0 37.0 22.0 39.0 76-77 31.019085864465072 34.0 30.5 36.0 21.0 39.0 78-79 31.400118198232253 34.0 31.0 36.0 21.0 39.0 80-81 31.068271291789983 34.0 31.0 36.0 20.0 37.5 82-83 30.710192038121257 34.0 30.5 35.0 19.0 37.0 84-85 30.370657951068367 34.0 30.0 35.0 18.0 37.0 86-87 30.244680005670077 34.0 30.0 35.0 18.0 36.0 88-89 29.930198431278214 34.0 30.0 35.0 14.5 36.0 90-91 29.54497457055597 34.0 29.5 35.0 8.5 35.5 92-93 29.276460009478768 34.0 29.0 35.0 4.5 35.0 94-95 28.860318111462867 34.0 29.0 35.0 2.0 35.0 96-97 28.43198447028435 34.0 29.0 35.0 2.0 35.0 98-99 28.19650420316064 34.0 29.0 35.0 2.0 35.0 100-101 27.129696965791958 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08284625176892746 1101 2 0.14452887583761864 1101 3 0.24160789412319872 1101 4 0.2937242001821474 1101 5 0.16811676391327524 1101 6 0.18918813847939475 1101 7 0.15394760708357325 1101 8 0.06118976512008345 1101 9 0.2772595154102504 1101 10-11 0.2255598344287364 1101 12-13 0.14542314713509796 1101 14-15 0.2868730055405351 1101 16-17 0.18914078544073476 1101 18-19 0.10215863121371882 1101 20-21 0.0743069078843206 1101 22-23 0.13818841630465784 1101 24-25 0.15203269118133278 1101 26-27 0.37067989956260305 1101 28-29 0.2017770614798593 1101 30-31 0.2042406933291332 1101 32-33 0.3634382080261602 1101 34-35 0.3005980438994982 1101 36-37 0.2630902353515978 1101 38-39 0.21326277113897163 1101 40-41 0.26289268177936975 1101 42-43 0.1273176230380315 1101 44-45 0.11811271768166165 1101 46-47 0.056067368036949006 1101 48-49 0.3025506181369906 1101 50-51 0.3390404304914725 1101 52-53 0.3312369549042131 1101 54-55 0.35074384402933845 1101 56-57 0.31488965773750266 1101 58-59 0.03309042952498231 1101 60-61 0.07545923319845826 1101 62-63 -0.052756227451659754 1101 64-65 -0.04734030850418236 1101 66-67 -0.13867897638663607 1101 68-69 -0.17761747014296958 1101 70-71 -0.029975614901665892 1101 72-73 -0.14099329352559664 1101 74-75 -0.10335020960357966 1101 76-77 -0.06369777469769033 1101 78-79 -0.007983935424025645 1101 80-81 0.040483597476800526 1101 82-83 0.05260861748228862 1101 84-85 0.0032773817402187433 1101 86-87 -0.0765042421773856 1101 88-89 -0.019386307471048525 1101 90-91 -0.05659693272582089 1101 92-93 0.08480705432360836 1101 94-95 0.0774941897778163 1101 96-97 0.32074948211623067 1101 98-99 -0.007533526247602396 1101 100-101 0.04798892179355718 1102 1 -0.04015587366893314 1102 2 -0.06694207034017197 1102 3 -0.01376111990568063 1102 4 0.023913564105981777 1102 5 -0.10840850312416705 1102 6 -0.024723852100542842 1102 7 -0.09496116201389526 1102 8 -0.11915499521889927 1102 9 -0.12348241298643359 1102 10-11 0.005878427936892194 1102 12-13 -0.040010444657404776 1102 14-15 0.046631443726333544 1102 16-17 0.018100061458831362 1102 18-19 -0.1180801781461085 1102 20-21 -0.2056330688617578 1102 22-23 -0.3150039370561757 1102 24-25 -0.19536105543981108 1102 26-27 -0.16396813187709114 1102 28-29 -0.0880481144673837 1102 30-31 -0.1806290156019017 1102 32-33 -0.1690650072848925 1102 34-35 -0.2025493815065218 1102 36-37 -0.2476132327409175 1102 38-39 -0.1701256112818612 1102 40-41 -0.15353761941072008 1102 42-43 -0.021925822294271313 1102 44-45 0.06155903741564117 1102 46-47 -0.11288037976417797 1102 48-49 -0.1640943868371778 1102 50-51 0.08056509272952184 1102 52-53 -0.07426912641538053 1102 54-55 -0.09809901427726686 1102 56-57 0.0032781882724677303 1102 58-59 -0.22500764918780902 1102 60-61 -0.1324570354114627 1102 62-63 -0.24479922745413063 1102 64-65 -0.3580676600012467 1102 66-67 -0.2295757581132989 1102 68-69 -0.22906398983104737 1102 70-71 -0.14629816305727417 1102 72-73 -0.06485386381439184 1102 74-75 -0.06943435571323775 1102 76-77 -0.2409124513274996 1102 78-79 -0.13996035812202834 1102 80-81 -0.18104590315638802 1102 82-83 -0.17852441863534452 1102 84-85 -0.09373657554860415 1102 86-87 -0.2701384200081556 1102 88-89 -0.2311220076682865 1102 90-91 -0.2626567140599825 1102 92-93 -0.26901392011929914 1102 94-95 -0.06894465266083571 1102 96-97 -0.03846297033296153 1102 98-99 -0.3283892309870069 1102 100-101 -0.2863682505100904 1103 1 -0.10767312539889673 1103 2 -0.09424147606956623 1103 3 -0.0529650974632645 1103 4 -0.0817123679344931 1103 5 -0.088020965882464 1103 6 -0.11435620903333188 1103 7 -0.05426272640492158 1103 8 0.03154417484595484 1103 9 -0.1171070166197552 1103 10-11 -0.12468866780102417 1103 12-13 -0.07129507185717898 1103 14-15 -0.07693037989568552 1103 16-17 -0.19766231971099302 1103 18-19 -0.07418685945931003 1103 20-21 -0.11546583645117892 1103 22-23 -0.04237710770220815 1103 24-25 -0.058136755807360885 1103 26-27 -0.05855615002773362 1103 28-29 -0.10015865327061135 1103 30-31 -0.0845685730762753 1103 32-33 0.020035028799249233 1103 34-35 0.04238846117302586 1103 36-37 -0.14693769299567805 1103 38-39 0.01111734352994631 1103 40-41 0.03784801143897454 1103 42-43 0.1197986162461433 1103 44-45 0.12618716324390533 1103 46-47 0.1460001498104475 1103 48-49 0.2911133843446905 1103 50-51 0.31717723935246767 1103 52-53 0.10675748324047163 1103 54-55 0.21014413532500953 1103 56-57 0.25831160601500613 1103 58-59 0.1708401204612855 1103 60-61 0.19542562408520325 1103 62-63 0.10762543493566312 1103 64-65 0.059238994488346464 1103 66-67 0.046817552590276534 1103 68-69 0.13995298887293472 1103 70-71 0.10675541709581182 1103 72-73 0.20944465098808251 1103 74-75 0.16367794897738364 1103 76-77 0.032463203695378695 1103 78-79 0.12515107160810857 1103 80-81 0.14963088197056607 1103 82-83 -0.013133736006505359 1103 84-85 -0.059994714437404895 1103 86-87 0.0660747254747811 1103 88-89 -0.0013434940153906894 1103 90-91 -0.07612487859087125 1103 92-93 0.009825447993179637 1103 94-95 -0.18806860646036583 1103 96-97 -0.019986615022364873 1103 98-99 -0.17216487706868477 1103 100-101 0.0999954584282392 1104 1 0.022134076828031368 1104 2 -0.0894619634058813 1104 3 -0.010852212259781169 1104 4 -0.0252548187303816 1104 5 -0.028551380294757678 1104 6 0.004746649117691959 1104 7 0.025423670913362173 1104 8 0.003329245025518901 1104 9 -0.041194976315516385 1104 10-11 0.015335248140317503 1104 12-13 0.04790911049207125 1104 14-15 -0.0033087320481186566 1104 16-17 -0.019366159003709527 1104 18-19 -0.0543093061385278 1104 20-21 0.03579109819165183 1104 22-23 -0.009861229085409207 1104 24-25 0.1392297952002437 1104 26-27 0.0951516785224058 1104 28-29 0.07312753429310703 1104 30-31 0.035933955740482304 1104 32-33 0.09009305436185855 1104 34-35 0.11190862309571514 1104 36-37 0.16516987483150558 1104 38-39 0.13221593307750368 1104 40-41 0.19082929525455228 1104 42-43 0.31440760530753664 1104 44-45 0.3735685391946575 1104 46-47 0.30564996259960253 1104 48-49 0.1834090696736368 1104 50-51 0.32937730992409087 1104 52-53 0.2910958497032681 1104 54-55 0.2293010248804066 1104 56-57 0.2103129733403435 1104 58-59 0.1587590169954325 1104 60-61 0.3670040027020036 1104 62-63 0.3592303269218604 1104 64-65 0.3146573274296216 1104 66-67 0.3240648179396075 1104 68-69 0.2205392969965061 1104 70-71 0.28393174325436377 1104 72-73 0.37613141096612424 1104 74-75 0.38738560599925975 1104 76-77 0.21229956572784303 1104 78-79 0.3011251071291632 1104 80-81 0.18161465539642307 1104 82-83 0.23413565648937862 1104 84-85 0.1367399999435257 1104 86-87 0.24423305096682313 1104 88-89 0.16603035919562004 1104 90-91 0.23709027176590425 1104 92-93 0.06787073635670282 1104 94-95 0.20491735762651686 1104 96-97 0.41073544547320395 1104 98-99 0.42064318770038867 1104 100-101 0.32718513928764636 1105 1 0.08480233894712796 1105 2 0.08146738763591799 1105 3 0.10371170621365877 1105 4 0.13669984823212644 1105 5 0.11143623117558832 1105 6 0.1315272691253 1105 7 0.009922292062086058 1105 8 0.08667420086001698 1105 9 0.09387195872526632 1105 10-11 0.136808347333087 1105 12-13 0.1356223437662365 1105 14-15 0.16775084383624517 1105 16-17 0.123830781637011 1105 18-19 0.12377891128677021 1105 20-21 0.05342302678722888 1105 22-23 0.15017110259964284 1105 24-25 0.15196369803613408 1105 26-27 0.25285667038665594 1105 28-29 0.1293701381559984 1105 30-31 0.14297956877458518 1105 32-33 0.16829558172756265 1105 34-35 0.19353120350228892 1105 36-37 0.24016346924847198 1105 38-39 0.4065222150393808 1105 40-41 0.408081775601957 1105 42-43 0.4232066971675579 1105 44-45 0.43914328508451916 1105 46-47 0.3273118613993091 1105 48-49 0.31236983316630074 1105 50-51 0.3471074354471071 1105 52-53 0.380614532873885 1105 54-55 0.35698681320295833 1105 56-57 0.32963888148031373 1105 58-59 0.2561360958382508 1105 60-61 0.2233675201991474 1105 62-63 0.1308651505768168 1105 64-65 0.2336400416006157 1105 66-67 0.22824059770378113 1105 68-69 0.1948494091008186 1105 70-71 0.4074615628942766 1105 72-73 0.3355062788922538 1105 74-75 0.28096919291443 1105 76-77 0.16855075439273648 1105 78-79 0.19718151324048705 1105 80-81 0.35545207509550636 1105 82-83 0.35774749400681216 1105 84-85 0.33274285009778737 1105 86-87 0.3273934995953667 1105 88-89 0.376854341698472 1105 90-91 0.44036986423875035 1105 92-93 0.40617024557018055 1105 94-95 0.48276909677532487 1105 96-97 0.5881897082896579 1105 98-99 0.40694950400147434 1105 100-101 0.5010176062163474 1106 1 -0.055809780544358034 1106 2 0.035122837607591606 1106 3 0.01905486041355786 1106 4 -0.019919801427207062 1106 5 0.11210220084477385 1106 6 0.05266331578435768 1106 7 0.03917390265232967 1106 8 0.09760374755919798 1106 9 -0.055886454501155924 1106 10-11 -0.07508311794545364 1106 12-13 -0.07105891725480973 1106 14-15 -0.03985744357945009 1106 16-17 -0.0035177240142090227 1106 18-19 0.019126262703395014 1106 20-21 -0.057377430111401395 1106 22-23 -0.10573627543320185 1106 24-25 -0.08557824768691091 1106 26-27 0.025414277397707963 1106 28-29 -0.02603279817506632 1106 30-31 -0.00408041207561638 1106 32-33 0.09477109168728504 1106 34-35 0.1051484111318004 1106 36-37 -0.03322166360626966 1106 38-39 0.19412132950563432 1106 40-41 -0.060879200296064084 1106 42-43 0.07775171450294494 1106 44-45 0.180242428392539 1106 46-47 -0.010288078124659705 1106 48-49 -0.03236347080343904 1106 50-51 0.16010126246395373 1106 52-53 0.15767117259289876 1106 54-55 0.09947332280402321 1106 56-57 0.19919471694434776 1106 58-59 0.18533464887035933 1106 60-61 0.2504387218343709 1106 62-63 -0.09330818037019384 1106 64-65 0.0714920820952969 1106 66-67 0.04306393733152447 1106 68-69 -0.03529917321731091 1106 70-71 0.05649674603522925 1106 72-73 0.23610931851773387 1106 74-75 0.11884880585094137 1106 76-77 0.0683143506740791 1106 78-79 0.13296720019553376 1106 80-81 0.10067537873099042 1106 82-83 0.1494252143314263 1106 84-85 0.015990285754920563 1106 86-87 0.13890807568564512 1106 88-89 0.0944994915649211 1106 90-91 0.04563545314027451 1106 92-93 -0.010292307457408612 1106 94-95 0.1040954567489969 1106 96-97 0.28747097723689663 1106 98-99 0.09114822416065138 1106 100-101 0.07567729106122201 1107 1 -0.05904179261747444 1107 2 -0.08021351485248474 1107 3 -0.06960848688171595 1107 4 -0.098289167652311 1107 5 -0.07579200277961462 1107 6 -0.06577613014269446 1107 7 -0.0875040359108894 1107 8 -0.1893392431347678 1107 9 -0.17364059625874972 1107 10-11 -0.06810504499306802 1107 12-13 -0.07696811077703813 1107 14-15 -0.20872357192612156 1107 16-17 -0.047763522603482045 1107 18-19 -0.041206796819260205 1107 20-21 -0.08158755273815643 1107 22-23 -0.21341631867748134 1107 24-25 -0.1410836446692869 1107 26-27 -0.09413334551707209 1107 28-29 -0.21156900698701975 1107 30-31 -0.13721304514908894 1107 32-33 -0.07695697317235073 1107 34-35 -0.16335017196507096 1107 36-37 -0.31511159623639884 1107 38-39 -0.18583346699450942 1107 40-41 -0.15357457594890178 1107 42-43 -0.08910995569132041 1107 44-45 -0.0540305971843722 1107 46-47 -0.1854987830055208 1107 48-49 -0.3424064988018145 1107 50-51 -0.0636697735680869 1107 52-53 -0.16221546950289678 1107 54-55 -0.19251261884814852 1107 56-57 -0.28995449234780324 1107 58-59 -0.20183351117417914 1107 60-61 -0.31291819992857484 1107 62-63 -0.420049984532362 1107 64-65 -0.3304542920052924 1107 66-67 -0.23045886677255112 1107 68-69 -0.24656665939354383 1107 70-71 -0.12712886641649845 1107 72-73 -0.16288217610550504 1107 74-75 -0.22508341801904308 1107 76-77 -0.31373812965911796 1107 78-79 -0.30054719875468194 1107 80-81 -0.19395926070896863 1107 82-83 -0.2916039943557074 1107 84-85 -0.31954858765761074 1107 86-87 -0.12809599867946986 1107 88-89 -0.15300557294315453 1107 90-91 -0.22120089981455848 1107 92-93 -0.20558887043365104 1107 94-95 -0.17360384371206905 1107 96-97 -0.10176885674647096 1107 98-99 0.00373639098755163 1107 100-101 0.22744141920462013 1108 1 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-0.8718234465270314 2316 62-63 -0.8966820487623934 2316 64-65 -0.946090238489063 2316 66-67 -0.9459438789389196 2316 68-69 -0.8304714866105272 2316 70-71 -0.955775187609369 2316 72-73 -0.8091478815832858 2316 74-75 -0.8296438223572693 2316 76-77 -0.7873654869235303 2316 78-79 -0.7760369682124413 2316 80-81 -0.7404374814762491 2316 82-83 -0.6989487189023755 2316 84-85 -0.7686854288935479 2316 86-87 -0.7650304305007296 2316 88-89 -0.8088873730345405 2316 90-91 -0.9656096127980511 2316 92-93 -1.1074736783146406 2316 94-95 -1.2692688443708953 2316 96-97 -1.0705893137303342 2316 98-99 -1.3340659239121635 2316 100-101 -1.7930960859014426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 23.0 5 43.0 6 94.0 7 266.0 8 689.0 9 1363.0 10 2216.0 11 3783.0 12 5635.0 13 8176.0 14 9841.0 15 12825.0 16 16147.0 17 20376.0 18 18852.0 19 14544.0 20 14114.0 21 14676.0 22 15418.0 23 17236.0 24 20409.0 25 24488.0 26 31354.0 27 39127.0 28 49553.0 29 62563.0 30 78215.0 31 97723.0 32 121442.0 33 154550.0 34 201206.0 35 271049.0 36 391469.0 37 512507.0 38 465861.0 39 95738.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.92369126963946 19.990831635156383 14.228012935989772 18.857464159214384 2 14.13011610336862 25.926172533765484 36.76642470857814 23.177286654287748 3 27.266453755583903 25.07891055800705 28.952922967395097 18.70171271901395 4 12.066851087409418 16.812893129038677 42.530494571185976 28.58976121236593 5 11.417977872374546 41.1549993341953 34.421745038323145 13.00527775510701 6 26.84442411212558 40.5239939260777 16.631908424381315 15.999673537415404 7 26.40585020396533 34.6317956824355 21.827941747100173 17.134412366499 8 23.812147141406896 29.688625335229084 19.41586734306697 27.08336018029705 9 21.555105003844485 23.703005284914298 24.78114355178887 29.960746159452352 10-11 25.441790346486055 25.222324777898947 27.752622105240736 21.583262770374258 12-13 30.699664090789984 23.65187076802362 25.587125198488554 20.06133994269784 14-15 22.49812066410361 27.330931363073375 30.2774078767442 19.89354009607881 16-17 19.479781014686363 29.811602327023223 29.51820080956124 21.19041584872917 18-19 18.96881170327161 34.073790490590675 27.95262906946276 19.004768736674958 20-21 22.6443048911879 27.20629181801958 26.376925895502364 23.77247739529016 22-23 20.159134482526564 28.55267660648651 27.762087218983883 23.52610169200304 24-25 24.710213873651746 28.124055881775995 27.353207961592375 19.812522282979884 26-27 23.389329570180017 24.923981582314326 32.462710343874605 19.223978503631056 28-29 19.359885870985526 33.319890596528154 28.421650861158543 18.89857267132777 30-31 21.7838758560991 27.660713576118045 26.884438301258818 23.670972266524043 32-33 22.168306453806974 28.664480363364035 28.535876041542863 20.63133714128612 34-35 25.21884114652087 26.412723528522065 28.92164856004718 19.44678676490988 36-37 23.444843313115808 25.582640554364 31.541205035304742 19.43131109721545 38-39 20.554331301754207 29.359668622708206 31.310453560227707 18.775546515309873 40-41 21.032455733191036 30.298372353331093 27.795100573552705 20.874071339925166 42-43 19.521179032736114 30.925429157663984 25.600477088443206 23.952914721156695 44-45 22.86169303396463 25.65587132357679 26.91152171454017 24.570913927918408 46-47 25.478619234116156 29.656888276061537 26.835161971485633 18.029330518336675 48-49 23.93848895265341 28.44473391437322 28.153748623645658 19.46302850932771 50-51 18.215808064971085 29.40084378217161 32.38185904126987 20.001489111587436 52-53 21.923320060194474 32.4344432942012 25.394560876743082 20.247675768861246 54-55 19.814150998778643 28.26740077004251 27.576968455175592 24.341479776003254 56-57 23.545884411021774 26.255544186231322 27.886820475307754 22.31175092743915 58-59 27.935788379644734 26.26348771740043 25.628967429736438 20.171756473218398 60-61 22.27433298040581 28.58057800757347 31.32836490140255 17.816724110618164 62-63 20.064532759549685 28.700233248781398 30.07529120295866 21.159942788710257 64-65 19.48693643009545 33.69426254341214 26.86417024360931 19.954630782883104 66-67 22.36146535042705 27.42087103755465 25.54357720931207 24.674086402706237 68-69 20.501364210086205 29.165136360154325 26.773526807032468 23.559972622727003 70-71 24.756528532602616 28.42671783118751 26.170670308320528 20.646083327889347 72-73 23.40734862250019 25.39175089454083 31.03133148053488 20.1695690024241 74-75 19.789279896163066 33.41753737367157 27.255799035728312 19.537383694437057 76-77 21.760180010681513 28.01946011113927 25.846932215354173 24.373427662825044 78-79 22.162669690708658 28.968249706831156 27.31104033058277 21.558040271877413 80-81 24.978271857366018 27.31879015410873 27.318217418882796 20.384720569642457 82-83 22.9219913144703 26.389133494558298 30.256134352229303 20.432740838742102 84-85 20.394836929577476 29.4771924059523 29.731469221354885 20.39650144311534 86-87 21.144687146624435 30.160245246891726 26.325363071857637 22.369704534626198 88-89 19.87480723880052 30.501193437027048 24.84772402180403 24.776275302368404 90-91 22.80980680208991 24.819713689660556 26.629789677306654 25.74068983094288 92-93 24.596597523206515 28.713418327240863 26.937706453150973 19.75227769640165 94-95 22.76646285608534 28.03028486111325 28.306504816174932 20.89674746662648 96-97 17.563240107116304 28.767675675714372 32.14996139388652 21.519122823282796 98-99 20.94072249133957 31.765062790899545 25.6975817024464 21.59663301531448 100-101 18.96562493903433 28.104989278096692 27.1892111217128 25.740174661156175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 155.0 1 124.0 2 188.0 3 293.5 4 260.0 5 231.5 6 305.5 7 527.0 8 836.0 9 1038.5 10 1103.5 11 1175.0 12 1358.0 13 1535.5 14 1712.0 15 1929.0 16 2144.5 17 2401.5 18 2681.0 19 2901.5 20 3254.5 21 3752.0 22 4251.5 23 5051.0 24 6112.0 25 7646.0 26 9887.5 27 13210.0 28 18529.0 29 25508.5 30 33759.5 31 42351.5 32 50784.5 33 58429.5 34 65280.0 35 71174.0 36 74817.0 37 77851.0 38 80290.0 39 80178.0 40 80132.0 41 80047.0 42 79998.0 43 82230.0 44 82866.0 45 84617.0 46 95840.0 47 180863.0 48 360989.5 49 348757.0 50 171979.5 51 91502.5 52 62721.5 53 48355.0 54 39725.5 55 33261.0 56 27046.5 57 21313.0 58 16804.0 59 13654.5 60 10928.0 61 8212.5 62 5993.5 63 4149.5 64 2644.0 65 1642.0 66 973.5 67 575.5 68 342.5 69 203.5 70 109.0 71 57.0 72 42.0 73 30.0 74 22.5 75 16.5 76 7.5 77 5.0 78 6.5 79 4.0 80 2.0 81 1.0 82 0.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05018592417271976 2 0.005476780598021486 3 0.04331310146147711 4 0.0 5 0.0 6 0.001073878548631664 7 0.0 8 0.0 9 0.0 10-11 2.68469637157916E-4 12-13 0.0 14-15 0.0025773085167159933 16-17 0.0 18-19 0.0 20-21 0.011937949865621997 22-23 0.0 24-25 0.0 26-27 0.007517149840421647 28-29 0.010094458357137641 30-31 0.009772294792548143 32-33 0.005727352259368875 34-35 0.007320272106505843 36-37 0.02052897825467531 38-39 0.010756683462127168 40-41 0.014586850285580103 42-43 0.0 44-45 0.0 46-47 1.0738785486316639E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0011633684276843026 58-59 1.9687773391580506E-4 60-61 0.011078847026716666 62-63 0.016358749890822345 64-65 0.042597182429056 66-67 0.023464246287601857 68-69 0.0019329813875369952 70-71 3.4006154040002693E-4 72-73 0.0 74-75 3.579595162105547E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.0738785486316639E-4 86-87 6.801230808000539E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.7897975810527735E-4 96-97 4.6534737107372103E-4 98-99 5.190412985053043E-4 100-101 0.001127572476063247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2793612.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.880186863776075 #Duplication Level Percentage of deduplicated Percentage of total 1 79.41757235598462 35.64275487604046 2 12.966119133101783 11.638436991831805 3 3.3210324827052204 4.471456752134414 4 1.3284200836643245 2.3847896635379175 5 0.7225051562167683 1.6213083210525143 6 0.44411642829898246 1.1959216974798683 7 0.30160543568398407 0.9475275818839459 8 0.20570742008090534 0.738574996199705 9 0.1641452187677423 0.6630181281982508 >10 0.9929445353360078 8.46525290187594 >50 0.085106219109225 2.6446111802665215 >100 0.042703405668801264 3.5872845696318394 >500 0.003770398929403839 1.129284169339008 >1k 0.0022461951068788555 2.5421314999900506 >5k 5.615487767197139E-4 1.7060234127440028 >10k+ 0.0014439825687078357 20.621623257793754 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 143561 5.1389026106703435 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 47239 1.6909649586270392 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 46184 1.6532002296668256 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 44348 1.5874788624905676 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 39004 1.3961852970276474 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 32160 1.1511978041331437 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 30955 1.108063682429772 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 27383 0.9802005432393618 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 24594 0.880365634168238 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 23752 0.8502254429033095 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 23041 0.824774521300739 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 19077 0.6828793690748751 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 14320 0.5125980272135143 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 12433 0.4450510665045826 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 12191 0.4363884462122871 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 11138 0.3986953091553158 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 10849 0.38835027913683073 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 10539 0.37725353413430357 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8631 0.30895485844132975 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 7358 0.2633866120277261 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 7279 0.2605587318496627 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 6555 0.23464246287601856 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 6314 0.2260156385353442 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5864 0.20990746030586926 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 5384 0.19272540352776263 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4992 0.17869339049230887 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4761 0.17042452566784508 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 4538 0.1624420284563497 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 4185 0.14980605753411713 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 4108 0.14704976925929586 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3616 0.12943816106173656 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3503 0.1253932185285573 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3205 0.11472602494548277 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.579595162105547E-5 0.0 10-11 0.0 0.0 0.0 7.159190324211093E-5 0.0 12-13 0.0 0.0 0.0 7.159190324211093E-5 0.0 14-15 0.0 0.0 0.0 1.6108178229474958E-4 0.0 16-17 0.0 0.0 0.0 2.3267368553686054E-4 0.0 18-19 0.0 0.0 0.0 4.1165344364213783E-4 0.0 20-21 0.0 0.0 0.0 7.875109356632202E-4 0.0 22-23 0.0 0.0 0.0 0.0012886542583579966 0.0 24-25 0.0 0.0 0.0 0.0018971854359159396 0.0 26-27 0.0 0.0 0.0 0.0030426558877897146 0.0 28-29 3.579595162105547E-5 0.0 0.0 0.0052799028641056815 0.0 30-31 3.579595162105547E-5 0.0 0.0 0.009647008961874447 0.0 32-33 3.579595162105547E-5 0.0 0.0 0.017718996052422456 0.0 34-35 3.579595162105547E-5 0.0 0.0 0.02605945278012838 0.0 36-37 3.579595162105547E-5 0.0 0.0 0.03379137833027636 0.0 38-39 3.579595162105547E-5 0.0 0.0 0.042400304695140195 0.0 40-41 3.579595162105547E-5 0.0 0.0 0.06022668860242582 0.0 42-43 3.579595162105547E-5 0.0 0.0 0.08095254459101694 0.0 44-45 3.579595162105547E-5 0.0 0.0 0.12449831973803091 0.0 46-47 3.579595162105547E-5 0.0 0.0 0.21019382791883767 0.0 48-49 3.579595162105547E-5 0.0 0.0 0.2628496727534103 0.0 50-51 3.579595162105547E-5 0.0 0.0 0.3257252617757942 0.0 52-53 3.579595162105547E-5 0.0 0.0 0.4034203747692951 0.0 54-55 3.579595162105547E-5 0.0 0.0 0.4863953906269016 0.0 56-57 3.579595162105547E-5 0.0 0.0 0.5787668437850353 0.0 58-59 3.579595162105547E-5 0.0 0.0 0.7438577726613431 0.0 60-61 3.579595162105547E-5 0.0 0.0 0.8953820358732709 0.0 62-63 3.579595162105547E-5 0.0 0.0 1.069439850630653 0.0 64-65 3.579595162105547E-5 0.0 0.0 1.1662857977414187 0.0 66-67 3.579595162105547E-5 0.0 0.0 1.4789455371755276 0.0 68-69 3.579595162105547E-5 0.0 0.0 1.8138345625663121 0.0 70-71 3.579595162105547E-5 0.0 0.0 1.9336794085936058 0.0 72-73 3.579595162105547E-5 0.0 0.0 2.2497934573591465 0.0 74-75 3.579595162105547E-5 0.0 0.0 2.4220793725112864 0.0 76-77 3.579595162105547E-5 0.0 0.0 2.531507596616853 0.0 78-79 3.579595162105547E-5 0.0 0.0 2.677447691375896 0.0 80-81 3.579595162105547E-5 0.0 0.0 2.8257324209661183 0.0 82-83 3.579595162105547E-5 0.0 0.0 3.0724023235868114 0.0 84-85 3.579595162105547E-5 0.0 0.0 3.5754249337416937 0.0 86-87 3.579595162105547E-5 0.0 0.0 4.478861058729702 0.0 88-89 3.579595162105547E-5 0.0 0.0 5.575792200205326 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2765 0.0 46.225494 2 ACCTGGG 2950 0.0 41.393078 3 TATAACG 430 0.0 39.77942 2 GTACCTG 3565 0.0 36.799767 1 TCACGCA 295 0.0 35.419178 4 ATAACGC 540 0.0 33.435394 3 GTATAAC 780 0.0 29.860497 1 CCTGGGG 1995 0.0 29.758081 4 TAACGCA 615 0.0 29.345823 4 GTACCGG 215 1.8189894E-12 28.740904 1 GTACACG 305 0.0 28.052282 1 TATCACG 390 0.0 26.802944 2 TACGCAG 1710 0.0 25.552273 4 AGTACGC 1735 0.0 25.194906 2 GAGTACG 1845 0.0 24.990267 1 GTACGCA 1785 0.0 24.488728 3 TACCGGG 230 3.619789E-9 22.724234 2 TACACGG 360 0.0 22.437311 2 ACGCAGT 1955 0.0 22.350468 5 CTAACGC 150 3.0285875E-5 22.172945 3 >>END_MODULE