##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003800_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2057817 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.650869829532947 31.0 31.0 34.0 30.0 34.0 2 31.850972170994797 31.0 31.0 34.0 30.0 34.0 3 31.899529938765205 31.0 31.0 34.0 30.0 34.0 4 35.59726885335285 37.0 35.0 37.0 33.0 37.0 5 35.4993796824499 37.0 35.0 37.0 33.0 37.0 6 35.565471565255805 37.0 35.0 37.0 33.0 37.0 7 35.50953899204837 37.0 35.0 37.0 33.0 37.0 8 35.51726951424738 37.0 35.0 37.0 33.0 37.0 9 37.0621488694087 39.0 37.0 39.0 33.0 39.0 10-11 36.97318639120971 39.0 37.0 39.0 33.0 39.0 12-13 36.94332610722917 39.0 37.0 39.0 33.0 39.0 14-15 37.934125337675795 40.0 37.0 41.0 33.0 41.0 16-17 38.02331815705673 40.0 37.0 41.0 33.0 41.0 18-19 38.07442085472129 40.0 37.0 41.0 33.0 41.0 20-21 37.99646324235829 40.0 37.0 41.0 33.0 41.0 22-23 37.89031216089671 40.0 37.0 41.0 33.0 41.0 24-25 37.73295560295206 39.5 37.0 41.0 32.0 41.0 26-27 37.452999950918866 39.0 36.5 41.0 31.5 41.0 28-29 37.35679824785197 39.0 36.0 41.0 32.0 41.0 30-31 37.19673250828426 39.0 36.0 40.0 31.0 41.0 32-33 36.941837879655964 39.0 36.0 40.0 31.0 41.0 34-35 36.86727026747276 39.0 35.0 40.0 30.0 41.0 36-37 36.650199215965266 38.5 35.0 40.0 30.5 41.0 38-39 36.41749655095667 38.0 35.0 40.0 30.0 41.0 40-41 36.71112494454074 39.0 35.0 40.0 30.0 41.0 42-43 36.594055496674386 39.0 35.0 40.5 30.0 41.0 44-45 36.43944869733315 39.0 35.0 41.0 30.0 41.0 46-47 36.19535945130204 39.0 35.0 40.0 30.0 41.0 48-49 35.90416178892487 39.0 35.0 40.0 28.0 41.0 50-51 35.612733785365755 38.0 35.0 40.0 28.0 41.0 52-53 35.53651393685639 38.0 34.5 40.0 28.0 41.0 54-55 35.35815891306176 38.0 34.0 40.0 27.0 41.0 56-57 35.02906162209759 38.0 34.0 40.0 26.5 41.0 58-59 34.750986846740986 38.0 34.0 40.0 26.0 41.0 60-61 34.13997260203409 37.5 33.0 40.0 24.0 41.0 62-63 34.051571155258216 37.0 33.0 40.0 25.0 41.0 64-65 33.63956221568779 36.5 32.5 39.5 23.5 41.0 66-67 33.23367189599464 36.0 32.0 39.0 22.5 40.0 68-69 32.868414927080494 36.0 32.0 39.0 22.0 40.0 70-71 32.3264456946366 35.0 31.0 38.0 21.5 40.0 72-73 31.88025392928526 35.0 31.0 37.0 20.5 39.5 74-75 31.556200818634505 35.0 31.0 37.0 20.0 39.0 76-77 30.62663541024299 34.0 29.5 36.0 20.0 38.5 78-79 30.997583847348913 34.0 31.0 36.0 20.0 38.5 80-81 30.718621480918856 34.0 30.5 36.0 18.0 37.0 82-83 30.374461140130535 34.0 30.0 35.0 16.0 37.0 84-85 30.03445593072659 34.0 30.0 35.0 14.0 36.5 86-87 29.838468629620614 34.0 30.0 35.0 10.5 36.0 88-89 29.524646749443704 34.0 29.5 35.0 7.0 36.0 90-91 29.157600991730554 34.0 29.0 35.0 3.5 35.5 92-93 28.906075224376124 34.0 29.0 35.0 2.0 35.0 94-95 28.509948649466885 34.0 29.0 35.0 2.0 35.0 96-97 28.033424740878317 34.0 29.0 35.0 2.0 35.0 98-99 27.743793544323914 34.0 29.0 35.0 2.0 35.0 100-101 26.659363053177227 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04494157824087708 1101 2 -0.004868712140577713 1101 3 0.07942770968135804 1101 4 0.06313038120351422 1101 5 0.1056318565155081 1101 6 0.13831590571899 1101 7 0.032676391568152496 1101 8 0.10709550033370618 1101 9 0.2032120829474593 1101 10-11 0.22769952369672097 1101 12-13 0.1918271417171269 1101 14-15 0.14381703373552313 1101 16-17 0.2538505302205252 1101 18-19 0.011363074883625757 1101 20-21 0.04617191540949506 1101 22-23 0.057781837094530886 1101 24-25 0.058955949925525886 1101 26-27 0.07044477438372354 1101 28-29 0.03488294330184516 1101 30-31 0.06526544176252003 1101 32-33 0.23442280369445712 1101 34-35 0.1622519561624287 1101 36-37 0.09202324706525644 1101 38-39 0.08511829408180205 1101 40-41 0.1708204679184533 1101 42-43 -0.08258350284550176 1101 44-45 -0.026055330427432466 1101 46-47 0.01091407444737058 1101 48-49 0.08348599344250118 1101 50-51 -0.0012541199509996659 1101 52-53 0.04193197361453116 1101 54-55 -0.00641169374291195 1101 56-57 0.026978585323362836 1101 58-59 -0.1262458000384541 1101 60-61 -0.06276864729160536 1101 62-63 -0.26596094614726695 1101 64-65 -0.3342028831464674 1101 66-67 -0.2730946684534459 1101 68-69 -0.2950385586586961 1101 70-71 -0.304926259709525 1101 72-73 -0.10109677787206195 1101 74-75 -0.2628675800340474 1101 76-77 -0.20843552157442602 1101 78-79 -0.11532953580192995 1101 80-81 -0.0462938219210578 1101 82-83 -0.23300841692511298 1101 84-85 -0.28279630193876315 1101 86-87 -0.3150317940102241 1101 88-89 -0.21996725525311334 1101 90-91 -0.15922615260230444 1101 92-93 -0.021649399855132145 1101 94-95 -0.14154652188151928 1101 96-97 -0.08444750739718998 1101 98-99 -0.3533783182585317 1101 100-101 -0.28342228657664137 1102 1 -0.1190057223303711 1102 2 -0.061595103150871466 1102 3 -0.023266836771110633 1102 4 -0.07504309743256954 1102 5 -0.09058057654736729 1102 6 -0.1690238875206731 1102 7 -0.16122190372431788 1102 8 -0.11761192621148808 1102 9 -0.14447368900954416 1102 10-11 -0.0991238020176084 1102 12-13 -0.08634354749602124 1102 14-15 -0.06525676049352569 1102 16-17 -0.10118207623794007 1102 18-19 -0.2953757286073966 1102 20-21 -0.08784586467925948 1102 22-23 -0.2089201712899893 1102 24-25 -0.1014439937443754 1102 26-27 -0.17053496303137905 1102 28-29 -0.17009179842828814 1102 30-31 -0.16287302013702742 1102 32-33 -0.20102372063038842 1102 34-35 -0.23817173822514803 1102 36-37 -0.25758487963412335 1102 38-39 -0.4381921190416733 1102 40-41 -0.25328150038503594 1102 42-43 -0.28912763350243154 1102 44-45 -0.050192866130238656 1102 46-47 -0.18426676862637947 1102 48-49 -0.24450117160106544 1102 50-51 -0.20485355881659473 1102 52-53 -0.1916436062062914 1102 54-55 -0.15321342625440337 1102 56-57 -0.03647108229296947 1102 58-59 -0.13045679054539505 1102 60-61 -0.14210967546026865 1102 62-63 -0.3641141044240044 1102 64-65 -0.20443807031764294 1102 66-67 -0.2707877524900013 1102 68-69 -0.13222066374321173 1102 70-71 -0.22891064796831273 1102 72-73 -0.1619675833563008 1102 74-75 0.02824548999220866 1102 76-77 0.09680791651121723 1102 78-79 -0.07602688251005318 1102 80-81 -0.14811859199678068 1102 82-83 -0.15521059863299413 1102 84-85 -0.17328075880471516 1102 86-87 -0.41818785906426115 1102 88-89 -0.2810380145177902 1102 90-91 -0.2184492576904269 1102 92-93 -0.16748228182552793 1102 94-95 -0.23535079827451355 1102 96-97 -0.2837181500296815 1102 98-99 -0.5453199552255157 1102 100-101 -0.4827547659313005 1103 1 -0.16482545385494518 1103 2 -0.13617098817108086 1103 3 -0.12559508047568357 1103 4 -0.0646176378346155 1103 5 -0.03144258884315576 1103 6 -0.04611551577857398 1103 7 -0.01185723580765341 1103 8 -0.026232049169351512 1103 9 -0.08931259628317889 1103 10-11 -0.052393651943283714 1103 12-13 0.0010169483692763492 1103 14-15 -0.008783264411597713 1103 16-17 -0.018546886086518555 1103 18-19 -0.054572823485152355 1103 20-21 -0.06805663357675229 1103 22-23 -0.062275086989899364 1103 24-25 -0.01980232701546214 1103 26-27 0.027592189291453906 1103 28-29 -0.026433353852702623 1103 30-31 0.07896075891232357 1103 32-33 0.08890116750484367 1103 34-35 -0.259506813337012 1103 36-37 -0.29655359485145993 1103 38-39 -0.18407562655286114 1103 40-41 0.022309197845295614 1103 42-43 -0.02335451540761113 1103 44-45 -0.052080934138039936 1103 46-47 0.07298810001190503 1103 48-49 0.12205434618898181 1103 50-51 0.1654636216685077 1103 52-53 0.10627102174532865 1103 54-55 -0.02629361031769406 1103 56-57 -0.041014304296901116 1103 58-59 -0.14593997534821312 1103 60-61 -0.0788164441187078 1103 62-63 -0.10854374354342866 1103 64-65 -0.0913832285717362 1103 66-67 -0.15920405374424007 1103 68-69 -0.15043650483922733 1103 70-71 0.0024360431504888425 1103 72-73 0.04402791964294295 1103 74-75 -0.11327697260126257 1103 76-77 -0.024679735411961445 1103 78-79 0.0075817551676458095 1103 80-81 0.08269632131269944 1103 82-83 -0.029161983216202003 1103 84-85 -0.10955239408450623 1103 86-87 -0.09200263417687538 1103 88-89 -0.053587447910636854 1103 90-91 0.04573973212782434 1103 92-93 -0.06984852966853694 1103 94-95 -0.24579353248084956 1103 96-97 -0.07550356017557291 1103 98-99 -0.06952868478545327 1103 100-101 -0.037520410190150955 1104 1 -0.06250207429599897 1104 2 -0.05988816500949312 1104 3 -0.08385663639868923 1104 4 -0.019230308684051067 1104 5 -0.0448709660303237 1104 6 -0.0710133950081584 1104 7 0.016615590561109173 1104 8 -0.020730874804499422 1104 9 -0.2165953099782172 1104 10-11 -0.0856378756621865 1104 12-13 -0.08096557731214915 1104 14-15 0.015401641879272177 1104 16-17 -0.17560114116663073 1104 18-19 -0.18089170339265337 1104 20-21 -0.11326223864831775 1104 22-23 -0.06354307455296038 1104 24-25 0.1359625753192546 1104 26-27 0.16934945393356315 1104 28-29 0.10184008015145452 1104 30-31 -0.005653285070188474 1104 32-33 0.007939609469332254 1104 34-35 0.10470376958198813 1104 36-37 0.14981163549880705 1104 38-39 0.0849456272424618 1104 40-41 0.04505495505616608 1104 42-43 0.14930040376959397 1104 44-45 0.12200857301281332 1104 46-47 0.04790585606280473 1104 48-49 -0.07514052539907112 1104 50-51 0.1264601609890832 1104 52-53 0.024439012573182595 1104 54-55 -0.09762643280711814 1104 56-57 -0.14552533449238325 1104 58-59 -0.01642063913802616 1104 60-61 0.0760359968893809 1104 62-63 -0.0550261223092221 1104 64-65 -0.19062762129163247 1104 66-67 0.0053083303796555015 1104 68-69 0.07351741742416351 1104 70-71 -0.08884345760521484 1104 72-73 0.07990750259458679 1104 74-75 -0.08151030727892561 1104 76-77 -0.08552945511997478 1104 78-79 -0.01719489465653723 1104 80-81 -0.08548223207720795 1104 82-83 -0.04552147137365026 1104 84-85 -0.025894502026208244 1104 86-87 -0.07759818023252407 1104 88-89 -0.08985304449374354 1104 90-91 -0.22870923821442446 1104 92-93 -0.10594632554990469 1104 94-95 -0.2465934064982953 1104 96-97 -0.04166303181701636 1104 98-99 -0.09231093084705577 1104 100-101 -0.007941395504349913 1105 1 0.034554962039976544 1105 2 0.026620544838873883 1105 3 0.08123419187999659 1105 4 0.0861768062107302 1105 5 -0.0051206758056565604 1105 6 0.02628607800289018 1105 7 0.02242707739726768 1105 8 0.0785345109884048 1105 9 0.07622234811458384 1105 10-11 0.1319537024606987 1105 12-13 0.05327984630708471 1105 14-15 0.17821379852444608 1105 16-17 0.25596916469209674 1105 18-19 0.15752658379222595 1105 20-21 0.0029348623685194752 1105 22-23 0.07503551611291215 1105 24-25 0.05035911779928881 1105 26-27 0.18075757587299535 1105 28-29 0.2711199344407902 1105 30-31 0.26234336433063987 1105 32-33 0.272894533342658 1105 34-35 0.36386080483009664 1105 36-37 0.26239044684422197 1105 38-39 0.3114354860852657 1105 40-41 0.2884652620338315 1105 42-43 0.3450586944045355 1105 44-45 0.26687070269468194 1105 46-47 0.2407370346624873 1105 48-49 0.2158087385103471 1105 50-51 0.396139880384176 1105 52-53 0.316303630339128 1105 54-55 0.34011318709569593 1105 56-57 0.5268238302666504 1105 58-59 0.29735691277994647 1105 60-61 0.4551323608054787 1105 62-63 0.385968132252394 1105 64-65 0.3239877358284673 1105 66-67 0.3731616934891875 1105 68-69 0.23174425733980542 1105 70-71 0.32627922933910014 1105 72-73 0.5222984876800609 1105 74-75 0.5105600708445905 1105 76-77 0.3435916186880057 1105 78-79 0.3056347920251419 1105 80-81 0.5512392314943462 1105 82-83 0.5099347524886433 1105 84-85 0.5770695971428239 1105 86-87 0.6163834070128189 1105 88-89 0.43055204232500444 1105 90-91 0.568361163220306 1105 92-93 0.39123029733829284 1105 94-95 0.48702813120294053 1105 96-97 0.5723808896272828 1105 98-99 0.5137123772635377 1105 100-101 0.5592850512287377 1106 1 -0.05788469260686213 1106 2 0.01616709294867036 1106 3 0.05370536164424067 1106 4 0.07753828742124824 1106 5 0.08123637359148006 1106 6 0.07664460936089768 1106 7 0.13887825006915477 1106 8 0.16823066211089355 1106 9 0.11388103751716017 1106 10-11 -0.1285501642378577 1106 12-13 0.027418222059203856 1106 14-15 0.06263960903042687 1106 16-17 0.10382665258082113 1106 18-19 0.1489185483752422 1106 20-21 0.014917172632969766 1106 22-23 0.10382823386971296 1106 24-25 0.06206004308688762 1106 26-27 0.07956684844593553 1106 28-29 0.1415022897268443 1106 30-31 0.137073117509118 1106 32-33 0.221485498071047 1106 34-35 0.11997741076450552 1106 36-37 0.13094379978070947 1106 38-39 0.24041626280159534 1106 40-41 0.014534642189630631 1106 42-43 0.014309413108669844 1106 44-45 0.15827720550149849 1106 46-47 0.10456546486259555 1106 48-49 0.267055977420263 1106 50-51 0.5215086119475956 1106 52-53 0.42650805652481694 1106 54-55 0.48822485941350635 1106 56-57 0.4549721764264447 1106 58-59 0.4106917468331517 1106 60-61 0.47739225734959945 1106 62-63 0.31260872391120387 1106 64-65 0.15360444259249562 1106 66-67 0.16832749958680182 1106 68-69 0.19630381950478437 1106 70-71 0.24330817916347058 1106 72-73 0.37317382944556243 1106 74-75 0.42892090955940887 1106 76-77 0.3083411157123628 1106 78-79 0.5065249065019302 1106 80-81 0.5094652868052556 1106 82-83 0.5982052197283991 1106 84-85 0.4761391508193533 1106 86-87 0.5593912248254256 1106 88-89 0.46608627515231404 1106 90-91 0.600143596356201 1106 92-93 0.5203883465900425 1106 94-95 0.7138994585427731 1106 96-97 1.0001165378210288 1106 98-99 0.7830446976109542 1106 100-101 0.8484948753162307 1107 1 -0.0802336831622199 1107 2 -0.06553346936378901 1107 3 -0.07217871449913105 1107 4 -0.20157392545573316 1107 5 -0.1872440695873152 1107 6 -0.03676231929714646 1107 7 -0.09940903100866905 1107 8 -0.03022876187968393 1107 9 -0.18356443989434723 1107 10-11 -0.17741887645450305 1107 12-13 -0.2004151503832432 1107 14-15 -0.11544765869397366 1107 16-17 -0.1471375233752994 1107 18-19 -0.051012246494714475 1107 20-21 -0.09059336203345225 1107 22-23 -0.2932061406896125 1107 24-25 -0.22930809911552075 1107 26-27 -0.13281505194688492 1107 28-29 -0.23509482366014112 1107 30-31 -0.12772405023353173 1107 32-33 -0.20443924786051326 1107 34-35 -0.09854097333726486 1107 36-37 -0.14444247316841796 1107 38-39 -0.13554157581526027 1107 40-41 -0.026756135991305996 1107 42-43 0.038058020625541644 1107 44-45 -0.04861524313280796 1107 46-47 -0.30652395076961625 1107 48-49 -0.29285618913526434 1107 50-51 0.02928850971350272 1107 52-53 -0.14408865625920697 1107 54-55 -0.16532118319169342 1107 56-57 -0.296216957758638 1107 58-59 -0.08957809222474822 1107 60-61 -0.1276727935333426 1107 62-63 -0.14614551725787095 1107 64-65 -0.2761366975301698 1107 66-67 -0.273945780888738 1107 68-69 -0.3116353774996199 1107 70-71 -0.2400874378634299 1107 72-73 -0.14603655054822084 1107 74-75 -0.14872943498406954 1107 76-77 -0.08331365430247928 1107 78-79 -0.157037456656564 1107 80-81 -0.27429403116128626 1107 82-83 -0.283471323029314 1107 84-85 -0.3326899886960284 1107 86-87 -0.23178589681187134 1107 88-89 -0.28992020652591677 1107 90-91 -0.3595797382672252 1107 92-93 -0.38446235677843177 1107 94-95 -0.3277287569816174 1107 96-97 -0.15636158042001114 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-0.5465230560940384 2316 74-75 -0.4832961558389641 2316 76-77 -0.6874356134777457 2316 78-79 -0.6294627343437327 2316 80-81 -0.7654694491611984 2316 82-83 -0.7957921684185401 2316 84-85 -0.6209392422322395 2316 86-87 -0.5774816301159724 2316 88-89 -0.6271265237027066 2316 90-91 -0.7688024783076628 2316 92-93 -0.6475171203465067 2316 94-95 -0.8728472601715005 2316 96-97 -0.7169920039201827 2316 98-99 -0.6946720106274888 2316 100-101 -1.0285820451772558 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 18.0 5 32.0 6 100.0 7 251.0 8 673.0 9 1131.0 10 2008.0 11 3557.0 12 5482.0 13 7726.0 14 8553.0 15 10847.0 16 13146.0 17 16730.0 18 15325.0 19 12059.0 20 11252.0 21 11663.0 22 12220.0 23 13840.0 24 16275.0 25 19666.0 26 24450.0 27 31076.0 28 39376.0 29 49230.0 30 60672.0 31 75443.0 32 92825.0 33 116864.0 34 151768.0 35 201817.0 36 284197.0 37 366320.0 38 320722.0 39 60463.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.008062061635734 19.7707694596542 13.84022901661518 19.380939462094894 2 14.65496629265903 25.253971401245657 36.946181001034155 23.144881305061155 3 25.884015597858774 25.515168339127513 29.130766003435184 19.47005005957853 4 12.312951054442644 17.04947524488329 41.4230225525399 29.214551148134166 5 11.638255491134537 40.67475387753138 34.01915719425002 13.667833437084056 6 26.88887862979549 39.80532560337643 17.252253018400765 16.053542748427322 7 26.80136280339797 34.00263483098837 22.053272958674167 17.142729406939488 8 24.08440595057772 30.300264795168864 19.484580018534203 26.130749235719215 9 22.447331322464535 22.53611472740287 24.463302616316223 30.553251333816366 10-11 25.2548404065978 25.26477816802592 27.999654972586114 21.480726452790165 12-13 30.127265932782166 23.444820409200624 26.170986049779938 20.256927608237273 14-15 22.726087646374335 26.968789917097574 29.795111772125356 20.51001066440274 16-17 19.76740400142481 29.344591866040563 29.572357503121026 21.3156466294136 18-19 19.18207498528781 32.94559234373124 28.49988604428868 19.372446626692266 20-21 22.417028419904625 27.356831255479758 26.921026335894926 23.305113988720684 22-23 20.34541944205923 28.271221396266043 28.047003207768235 23.336355953906494 24-25 24.012679455947737 28.029606131157436 27.870189623275536 20.08752478961929 26-27 23.264579818527267 25.080181924701524 32.07673068179968 19.578507574971525 28-29 19.723258225901617 31.997204491088098 29.14286450238289 19.136672780627396 30-31 21.730214293264773 27.74325632934847 27.529193918576578 22.997335458810177 32-33 22.08755251090069 28.374013689283206 28.830598522210583 20.707835277605522 34-35 24.65575602940283 26.270068647105276 29.390024907356782 19.684150416135108 36-37 23.45134302604638 25.68723684770127 31.31082501473349 19.550595111518856 38-39 20.928257471782768 28.854548582626038 31.352084556436406 18.865109389154785 40-41 21.179125097051458 29.729252251168564 28.310423721502715 20.781198930277263 42-43 19.749982627220984 30.427414099504475 26.52351982707889 23.299083446195652 44-45 22.50885282802115 26.157889647135775 27.17962773171764 24.153629793125432 46-47 24.637995971942093 29.45363663554872 26.981136063947424 18.92723132856177 48-49 23.665393958743657 28.363916713682507 28.22945383384431 19.74123549372952 50-51 19.059323545290958 29.252333905298673 31.460158993729763 20.228183555680605 52-53 21.90627252083154 31.615663589133536 26.253427782936967 20.22463610709796 54-55 20.193267914493855 28.39407002663502 27.713251469882888 23.699410588988236 56-57 23.20674859430608 26.68078860273259 27.98983764876082 22.12262515420051 58-59 26.960533595488613 26.363905183827395 26.238577985808725 20.43698323487527 60-61 22.332888072509878 28.352875966145923 30.823818384128465 18.490417577215734 62-63 20.76208301751806 28.21676539868302 29.888085353842307 21.133066229956615 64-65 20.044273773027758 32.61582304880859 27.32674307016034 20.01316010800331 66-67 22.21293566677279 27.637890766452717 26.190362775143573 23.95881079163092 68-69 20.678804837352512 29.077935258824017 26.92201808892454 23.32124181489893 70-71 24.093617949757764 28.463019311059014 26.566318910317545 20.87704382886568 72-73 23.087232732551048 25.751706784422524 30.595504848098738 20.565555634927694 74-75 19.99949461006494 32.26946322243426 27.741363785020727 19.989678382480076 76-77 21.855903610476542 27.940701238253933 26.477427293097488 23.725967858172034 78-79 22.13428113384232 28.871080373036083 27.60835876076444 21.386279732357153 80-81 24.193793714407064 27.48201127699888 27.583210751976488 20.74098425661757 82-83 22.619018114827508 26.589657875311556 29.77942159093836 21.011902418922578 84-85 20.482730117092558 29.065954131478684 29.66615342038515 20.785162331043608 86-87 21.50470149360714 29.6995072658784 26.60263722945364 22.193154011060823 88-89 20.284675459479633 29.88008651886927 25.473110582719453 24.362127438931644 90-91 22.352862280756742 25.424126635167266 26.72839713152336 25.49461395255263 92-93 23.78088041842399 28.781446552341635 26.953222759846962 20.484450269387413 94-95 22.531344525298795 28.159934649146145 28.00139273904779 21.30732808650727 96-97 18.467794920041285 28.973103471065386 30.77086245001973 21.788239158873598 98-99 21.103692492578887 31.29709951426528 25.86907297829416 21.73013501486167 100-101 19.34874074131669 28.481075401958794 26.926962704794157 25.243221151930364 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 264.0 1 207.5 2 367.0 3 566.5 4 432.0 5 346.5 6 479.5 7 723.5 8 1015.0 9 1242.5 10 1335.0 11 1327.5 12 1390.0 13 1553.5 14 1733.5 15 1897.0 16 2051.5 17 2304.5 18 2529.0 19 2735.5 20 2960.5 21 3271.0 22 3788.0 23 4454.0 24 5313.5 25 6607.5 26 8364.0 27 10902.5 28 14626.5 29 19175.0 30 24340.5 31 29224.0 32 33896.5 33 38573.5 34 42814.0 35 46774.0 36 50364.0 37 53783.5 38 57262.0 39 59570.0 40 60946.5 41 61943.5 42 63516.0 43 66064.5 44 67000.5 45 69106.0 46 76516.5 47 126904.5 48 238882.0 49 233713.5 50 123960.5 51 72409.0 52 51797.0 53 40943.0 54 34104.0 55 28815.0 56 23859.0 57 19094.0 58 15286.5 59 12130.0 60 9418.0 61 7075.0 62 5066.5 63 3477.5 64 2187.0 65 1325.0 66 788.5 67 447.0 68 255.5 69 137.5 70 76.0 71 48.0 72 31.5 73 22.0 74 12.5 75 7.5 76 7.0 77 4.5 78 2.0 79 1.0 80 1.5 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04971287534314276 2 0.005102494536686207 3 0.04218062150327264 4 0.0 5 0.0 6 0.0010690940934009194 7 0.0 8 0.0 9 0.0 10-11 4.6165426760494253E-4 12-13 0.0 14-15 0.0017737242913242529 16-17 0.0 18-19 0.0 20-21 0.011905820585601148 22-23 0.0 24-25 0.0 26-27 0.007410765874710919 28-29 0.011225487980709655 30-31 0.011638547062250919 32-33 0.007240682723488046 34-35 0.009330275724226208 36-37 0.02087163241434977 38-39 0.010156393887308735 40-41 0.013339378574479656 42-43 0.0 44-45 0.0 46-47 7.289277909551725E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0012391772446237932 58-59 1.7008315122287357E-4 60-61 0.010763833713104713 62-63 0.015137400458835746 64-65 0.04096574185168069 66-67 0.02342287968269287 68-69 0.001870914663451609 70-71 3.1586870941390806E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.429759303183908E-5 86-87 6.560350118596551E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 7.289277909551725E-5 96-97 2.429759303183908E-4 98-99 3.1586870941390806E-4 100-101 9.233085352098851E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2057817.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.633615586732816 #Duplication Level Percentage of deduplicated Percentage of total 1 81.43134381339367 40.41732015545054 2 11.955289510194982 11.86768487554234 3 2.8381903732868525 4.22608949849056 4 1.1503142643074595 2.2837702399428728 5 0.6019422236952732 1.493828446815716 6 0.37711290726988983 1.1230486243337359 7 0.2593274711746633 0.9009952010753939 8 0.19137067860098783 0.7598734957002901 9 0.15018634445766632 0.6708862158469606 >10 0.9192709260899096 8.695051099788955 >50 0.08090509921175967 2.7455877728506035 >100 0.03735489394998066 3.4981762234642506 >500 0.0021681716809675735 0.7488969990940545 >1k 0.0032522575214513597 3.7317669536829143 >5k 7.884260658063903E-4 2.477376709086707 >10k+ 0.0011826390987095855 14.359647488834138 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 85629 4.1611571874466975 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 28521 1.3859833017221648 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 28460 1.3830189953722805 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 25570 1.2425789076482505 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 22001 1.0691426885869832 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 18850 0.9160192573003333 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 17641 0.8572676773493465 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 15536 0.7549748106853039 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 14152 0.6877190731731734 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 13654 0.6635186705134616 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 12571 0.6108900840064981 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10975 0.5333321670488678 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8730 0.42423597433591037 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 6983 0.3393401842826646 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6910 0.3357927357000161 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 6166 0.29963791726863953 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5875 0.2854967181241092 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5698 0.27689537019083815 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5213 0.25332670494995424 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5071 0.24642618852891196 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4617 0.22436397405600209 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4553 0.22125388214792666 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4541 0.22067073991516253 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4478 0.2176092431931508 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4206 0.20439135258383034 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3742 0.18184318625028367 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3690 0.17931623657497242 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3518 0.17095786457201975 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3317 0.16119023217322045 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2610 0.1268334356262 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2566 0.12469524743939815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2466 0.11983572883303034 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2288 0.11118578571369563 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2283 0.11094280978337723 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.859518606367816E-5 0.0 5 0.0 0.0 0.0 4.859518606367816E-5 0.0 6 0.0 0.0 0.0 4.859518606367816E-5 0.0 7 0.0 0.0 0.0 4.859518606367816E-5 0.0 8 0.0 0.0 0.0 9.719037212735632E-5 0.0 9 0.0 0.0 0.0 9.719037212735632E-5 0.0 10-11 0.0 0.0 0.0 1.457855581910345E-4 0.0 12-13 0.0 0.0 0.0 1.9438074425471265E-4 0.0 14-15 0.0 0.0 0.0 2.672735233502299E-4 0.0 16-17 0.0 0.0 0.0 4.616542676049425E-4 0.0 18-19 0.0 0.0 0.0 7.289277909551724E-4 0.0 20-21 0.0 0.0 0.0 0.0010933916864327587 0.0 22-23 0.0 0.0 0.0 0.0016765339191968966 0.0 24-25 0.0 4.859518606367816E-5 0.0 0.002454056896215747 0.0 26-27 0.0 4.859518606367816E-5 0.0 0.0032315798732345975 0.0 28-29 0.0 4.859518606367816E-5 0.0 0.007192087537424368 0.0 30-31 0.0 4.859518606367816E-5 0.0 0.016789636785000804 0.0 32-33 0.0 4.859518606367816E-5 0.0 0.035182914710102986 0.0 34-35 0.0 4.859518606367816E-5 0.0 0.055471404891688614 0.0 36-37 0.0 7.289277909551725E-5 0.0 0.0702443414550468 0.0 38-39 0.0 9.719037212735632E-5 0.0 0.08382669595984482 0.0 40-41 0.0 9.719037212735632E-5 0.0 0.09507648153358632 0.0 42-43 0.0 1.457855581910345E-4 0.0 0.11031107236454943 0.0 44-45 0.0 1.457855581910345E-4 0.0 0.1230430111132331 0.0 46-47 0.0 1.457855581910345E-4 0.0 0.14340439407391425 0.0 48-49 0.0 1.457855581910345E-4 0.0 0.1619677551502393 0.0 50-51 0.0 1.457855581910345E-4 0.0 0.17863590397008092 0.0 52-53 0.0 1.457855581910345E-4 0.0 0.21279831977284666 0.0 54-55 0.0 1.457855581910345E-4 0.0 0.290356236730477 0.0 56-57 0.0 1.457855581910345E-4 0.0 0.4626018737331843 0.0 58-59 0.0 1.457855581910345E-4 0.0 0.6318103116069116 0.0 60-61 0.0 1.457855581910345E-4 0.0 0.7518890163702603 0.0 62-63 0.0 1.457855581910345E-4 0.0 0.8989623469919823 0.0 64-65 0.0 1.457855581910345E-4 0.0 1.0975222772481712 0.0 66-67 0.0 1.457855581910345E-4 0.0 1.548582794291232 0.0 68-69 0.0 1.457855581910345E-4 0.0 2.0221914776678394 0.0 70-71 0.0 1.457855581910345E-4 0.0 2.268350392673401 0.0 72-73 0.0 1.9438074425471265E-4 0.0 2.4849148393661826 0.0 74-75 0.0 1.9438074425471265E-4 0.0 2.745506524632657 0.0 76-77 0.0 1.9438074425471265E-4 0.0 2.997083802884319 0.0 78-79 0.0 1.9438074425471265E-4 0.0 3.1665595142813965 0.0 80-81 0.0 1.9438074425471265E-4 0.0 3.361110341687332 0.0 82-83 0.0 1.9438074425471265E-4 0.0 3.632125694364465 0.0 84-85 0.0 1.9438074425471265E-4 0.0 4.280434071639995 0.0 86-87 0.0 1.9438074425471265E-4 0.0 5.190646204205719 0.0 88-89 0.0 1.9438074425471265E-4 0.0 6.170956892668299 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 370 0.0 59.062737 2 TACCTGG 2245 0.0 48.459248 2 ATAACGC 495 0.0 44.146835 3 ACCTGGG 2500 0.0 42.565228 3 GTACCCG 195 0.0 41.434475 1 TAACGCA 495 0.0 41.256664 4 GTACCTG 2770 0.0 40.149822 1 AGTACGC 1105 0.0 39.983257 2 GTACGCA 1155 0.0 37.840145 3 TACGCAG 1195 0.0 36.961185 4 GTATAAC 690 0.0 35.81804 1 GAGTACG 1250 0.0 35.74089 1 CGCAGTG 1485 0.0 30.062996 6 ACGCAGT 1470 0.0 30.046679 5 CCTGGGG 1705 0.0 28.969421 4 TCACGCA 235 5.456968E-12 26.272799 4 TACCCGG 245 1.0913936E-11 25.207788 2 TATCACG 250 1.4551915E-11 24.703632 2 GTACACG 205 2.6553607E-8 23.184282 1 GTACCGG 130 2.9309918E-4 21.935898 1 >>END_MODULE