FastQCFastQC Report
Wed 25 May 2016
SRR1003799_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003799_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593843
Sequences flagged as poor quality0
Sequence length121
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT128862.169933804052586No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT102481.725708646898254No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC82551.3900980562202467No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT69841.17606842212504No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT41990.7070892474947083No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28600.4816087753833926No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27680.4661164651263044No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22330.3760253130878027No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC19350.3258436994289736No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18840.31725557091689216No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG18220.30681510096102843No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA17770.2992373405091918No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT16580.2791983739810017No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA15620.26303248501708365No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15030.2530971990913423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.24501425460938328No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC13900.2340686006233971No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13880.23373181126998213No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG13210.22244936793058095No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC13090.2204286318100912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.1793403306934661No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC10230.17226775427175195No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG10050.16923665009101732No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8420.1417883177876981No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG8290.13959918699050086No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT7960.13404216265915403No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT6950.11703430031169855No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6910.11636072160486861No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.10676222503254228No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT6150.10356272617510016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG7250.057.129882
GTACCCG1350.055.481191
ACCTGGG7800.053.020083
TATAACG1058.589268E-943.8298032
GTACCGG706.587905E-541.1536251
GTACCTG10650.039.4920271
GTACGCA1602.4738256E-1035.8990143
TAACGCA1305.723814E-835.346724
TACCGGG852.0841556E-433.8391842
GAGTACG1904.1836756E-1133.35611
TACCCGG1904.1836756E-1133.3048822
AGTACGC1756.384653E-1032.872352
CCTGGGG5800.032.680484
GTATAAC1501.9577419E-730.7280431
TACGCAG1952.068191E-929.45564
GACAACG800.006365373328.7216577
ACAACGC800.006368022428.7192123
ATAACGC1854.0345185E-827.9430143
GGATGGC3300.026.1039268
GGGATGG3500.024.6185637
Produced by FastQC (version 0.11.5)