##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003799_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 593843 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.78731920726522 31.0 31.0 34.0 28.0 34.0 2 30.698575212640378 31.0 31.0 34.0 27.0 34.0 3 30.807533304257184 31.0 31.0 34.0 27.0 34.0 4 34.3186566146271 37.0 35.0 37.0 30.0 37.0 5 34.30924672009269 37.0 35.0 37.0 30.0 37.0 6 34.406753973693384 37.0 35.0 37.0 30.0 37.0 7 34.39644653553212 37.0 35.0 37.0 30.0 37.0 8 34.36986880370738 37.0 35.0 37.0 30.0 37.0 9 35.82613081235276 39.0 35.0 39.0 30.0 39.0 10-11 35.689084488661145 39.0 35.0 39.0 30.0 39.0 12-13 35.56769466003641 39.0 35.0 39.0 30.0 39.0 14-15 36.539136606813585 39.0 36.0 41.0 30.0 41.0 16-17 36.57972056587347 39.0 36.0 41.0 30.0 41.0 18-19 36.52471360275359 39.0 36.0 41.0 30.0 41.0 20-21 36.447056713643164 39.0 36.0 41.0 30.0 41.0 22-23 36.343386214874975 39.0 36.0 41.0 29.0 41.0 24-25 36.078104818950465 39.0 35.5 41.0 27.0 41.0 26-27 35.970741424922075 39.0 35.5 41.0 27.0 41.0 28-29 35.81669818453699 39.0 35.0 40.0 27.0 41.0 30-31 35.54354181155625 38.0 35.0 40.0 27.0 41.0 32-33 35.27479232726495 38.0 34.5 40.0 26.5 41.0 34-35 35.224999705309315 38.0 35.0 40.0 25.5 41.0 36-37 35.061702335465775 38.0 34.5 40.0 25.0 41.0 38-39 35.02489378505767 38.0 34.5 40.0 25.0 41.0 40-41 35.17533085344106 38.0 35.0 40.0 25.0 41.0 42-43 35.024105697970676 38.0 35.0 40.0 24.5 41.0 44-45 34.78941151112331 38.0 34.0 40.0 24.0 41.0 46-47 34.496659891587505 38.0 34.0 40.0 23.0 41.0 48-49 34.19943570943835 38.0 33.0 40.0 21.5 41.0 50-51 33.35257719632967 37.5 32.5 39.5 18.5 40.5 52-53 33.49687375282693 38.0 33.0 39.5 19.0 40.5 54-55 33.84547346689277 38.0 33.0 40.0 19.5 41.0 56-57 33.59422439937829 38.0 33.0 40.0 16.0 41.0 58-59 33.293283746714195 38.0 32.5 40.0 13.5 41.0 60-61 32.956227487736655 37.5 32.0 40.0 12.5 41.0 62-63 32.72112999563858 37.0 32.0 40.0 10.0 41.0 64-65 32.44382016795685 36.5 31.0 39.5 8.0 41.0 66-67 32.024658032510274 36.0 31.0 39.0 7.5 41.0 68-69 31.593970965389843 36.0 31.0 39.0 4.5 40.5 70-71 31.071378293589383 35.0 30.0 38.5 2.0 40.0 72-73 30.66916760827357 35.0 29.5 37.5 2.0 40.0 74-75 30.32262651913048 35.0 29.0 37.0 2.0 39.5 76-77 29.888553035061456 34.5 29.0 36.5 2.0 39.0 78-79 29.338021160475073 34.0 29.0 36.0 2.0 39.0 80-81 28.846231242937947 34.0 28.0 36.0 2.0 37.5 82-83 28.436841892554092 34.0 27.0 35.0 2.0 37.0 84-85 28.139419004686424 34.0 26.0 35.0 2.0 37.0 86-87 27.951454340625382 34.0 26.0 35.0 2.0 36.0 88-89 27.60383805147152 34.0 26.0 35.0 2.0 36.0 90-91 27.188603721859145 33.5 25.0 35.0 2.0 35.5 92-93 26.87259006168297 33.0 25.0 35.0 2.0 35.0 94-95 26.58602442059602 33.0 25.0 35.0 2.0 35.0 96-97 26.252281326882695 33.0 24.0 35.0 2.0 35.0 98-99 25.952318205316892 33.0 23.5 35.0 2.0 35.0 100-101 25.60133739052241 32.5 20.0 35.0 2.0 35.0 102-103 25.137947235212003 32.0 18.5 35.0 2.0 35.0 104-105 24.68606769802793 32.0 17.0 35.0 2.0 35.0 106-107 24.226950557638972 31.0 9.5 34.0 2.0 35.0 108-109 23.851833733832006 31.0 6.0 34.0 2.0 35.0 110-111 23.444190636245608 31.0 2.0 34.0 2.0 35.0 112-113 22.815550574815227 31.0 2.0 34.0 2.0 35.0 114-115 22.20023895204625 30.5 2.0 34.0 2.0 35.0 116-117 21.440074228373494 29.5 2.0 34.0 2.0 35.0 118-119 20.618065717706532 29.0 2.0 34.0 2.0 35.0 120-121 19.0660174490564 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.678130730318049 1101 2 0.4340823128162796 1101 3 0.6515736590466119 1101 4 0.8409788428054341 1101 5 0.7742346460015241 1101 6 0.9031868064816209 1101 7 0.6842303731124773 1101 8 0.5544523983797731 1101 9 0.768258565547363 1101 10-11 0.872837371929343 1101 12-13 0.8195279364223467 1101 14-15 0.9189875352346135 1101 16-17 0.7653541307641021 1101 18-19 0.6799161834816161 1101 20-21 0.7062920479501784 1101 22-23 0.6493742818177779 1101 24-25 0.7092553931002072 1101 26-27 0.8109900804995362 1101 28-29 0.8856585357021061 1101 30-31 0.9028534542965474 1101 32-33 0.7528410575096984 1101 34-35 0.9184049639970979 1101 36-37 1.0248920979041003 1101 38-39 1.0532455615164196 1101 40-41 1.2502806220738378 1101 42-43 1.3158843991621225 1101 44-45 1.2114562764306385 1101 46-47 1.2711493112899035 1101 48-49 1.4943604778764694 1101 50-51 1.435593564155461 1101 52-53 1.4784552767663186 1101 54-55 1.4580811428424099 1101 56-57 1.4671115638104695 1101 58-59 1.5465831177439924 1101 60-61 1.548266542613618 1101 62-63 1.4485893367586513 1101 64-65 1.35274423724589 1101 66-67 1.5783513283462653 1101 68-69 1.6060879687831147 1101 70-71 1.2176982134193572 1101 72-73 1.3448461246613554 1101 74-75 1.3823137712971452 1101 76-77 1.393987667630018 1101 78-79 1.6836532123335388 1101 80-81 1.8081428853252355 1101 82-83 1.8334936073797365 1101 84-85 1.7452663391606578 1101 86-87 1.8150649985057044 1101 88-89 1.9001977631297784 1101 90-91 1.8461256122877039 1101 92-93 1.8395460572938482 1101 94-95 2.0595663577478582 1101 96-97 2.0584134002368337 1101 98-99 2.148547224649384 1101 100-101 2.19130444682591 1101 102-103 2.1986172619671116 1101 104-105 2.3015247068339626 1101 106-107 2.3023123276039392 1101 108-109 2.356348616691424 1101 110-111 2.5090642573403628 1101 112-113 2.703802230010634 1101 114-115 2.608908642964593 1101 116-117 2.6654047927874487 1101 118-119 2.791696479465269 1101 120-121 2.561424435729897 1102 1 0.8644799999441766 1102 2 0.7772545747447701 1102 3 0.8107290386923225 1102 4 1.1773912311612165 1102 5 1.1946060182564935 1102 6 1.1565570935373728 1102 7 1.094391381240392 1102 8 1.157090413256178 1102 9 1.1276265556508562 1102 10-11 1.2500538834093575 1102 12-13 1.1638160868695593 1102 14-15 1.390413575586308 1102 16-17 1.158224403582551 1102 18-19 1.110250507098776 1102 20-21 1.3621810728844466 1102 22-23 1.2103359012007573 1102 24-25 1.2186159481653647 1102 26-27 1.279900863203416 1102 28-29 1.3552451159324903 1102 30-31 1.4607641768873592 1102 32-33 1.313631196434173 1102 34-35 1.5036563790272695 1102 36-37 1.2910000293498882 1102 38-39 1.378336126610968 1102 40-41 1.3412766324639094 1102 42-43 1.5189030247241035 1102 44-45 1.4670836512926186 1102 46-47 1.3469936627172743 1102 48-49 1.5945775177187826 1102 50-51 1.210861462823793 1102 52-53 1.2542157862720984 1102 54-55 1.4353058803222822 1102 56-57 1.556128588466315 1102 58-59 1.303246652203029 1102 60-61 1.574338532767726 1102 62-63 1.472790544715096 1102 64-65 1.6085595445660807 1102 66-67 1.3526403330319212 1102 68-69 1.2896334379973098 1102 70-71 1.3282173689255536 1102 72-73 1.430169991767162 1102 74-75 1.414604300956622 1102 76-77 1.2969952197306576 1102 78-79 1.3743407477290646 1102 80-81 1.4996802297826513 1102 82-83 1.4695067183579624 1102 84-85 1.3746537236407264 1102 86-87 1.630638081768641 1102 88-89 1.5030518686946657 1102 90-91 1.660698920183716 1102 92-93 1.555023592404634 1102 94-95 1.749263609314756 1102 96-97 1.7563031148895547 1102 98-99 2.0510067986058687 1102 100-101 2.143529737978845 1102 102-103 2.2327453534402295 1102 104-105 2.270061839313687 1102 106-107 2.132322356996074 1102 108-109 2.120979348394112 1102 110-111 2.215350186767612 1102 112-113 2.207160019874305 1102 114-115 2.1620894156390875 1102 116-117 2.098042079979251 1102 118-119 2.3199850601955454 1102 120-121 2.2387029585934357 1103 1 0.5530349448774352 1103 2 0.6756531940423329 1103 3 0.8360564176673009 1103 4 0.9997911033418347 1103 5 1.2347710444689568 1103 6 0.9922672662517371 1103 7 0.9265675378634342 1103 8 0.9764830497207697 1103 9 0.96768385290369 1103 10-11 1.0867262608182315 1103 12-13 1.0527654843150671 1103 14-15 1.1755967306369186 1103 16-17 1.2113311422583593 1103 18-19 1.204149899956711 1103 20-21 1.1928820862643548 1103 22-23 1.1903704504001524 1103 24-25 1.2216117149392858 1103 26-27 1.1686529157485737 1103 28-29 1.1656393786139816 1103 30-31 1.1984473240283506 1103 32-33 1.2743927816476273 1103 34-35 1.1995352321963324 1103 36-37 1.1130147032680853 1103 38-39 1.0985520749263813 1103 40-41 1.355931963070013 1103 42-43 1.4425127516525436 1103 44-45 1.467778115511102 1103 46-47 1.521436667611745 1103 48-49 1.6924447690642097 1103 50-51 1.5618387748834266 1103 52-53 1.5446430162299265 1103 54-55 1.433751640926694 1103 56-57 1.4396211423545324 1103 58-59 1.4959126196597055 1103 60-61 1.286197577096381 1103 62-63 1.569949490015361 1103 64-65 1.7796599460581497 1103 66-67 2.031608033327103 1103 68-69 1.7378887350666474 1103 70-71 1.5506484049902376 1103 72-73 1.2501143238950725 1103 74-75 1.1552064916036606 1103 76-77 1.334600694449943 1103 78-79 1.395051679766489 1103 80-81 1.767050931302247 1103 82-83 1.6995855614027207 1103 84-85 1.6086763008464828 1103 86-87 1.906061167088076 1103 88-89 1.8966537018270948 1103 90-91 1.844209903475445 1103 92-93 1.737821919362812 1103 94-95 1.8375357064068645 1103 96-97 1.8154057374015835 1103 98-99 1.9499840062585818 1103 100-101 1.7791396958680536 1103 102-103 1.8399965723119394 1103 104-105 1.9547814118148032 1103 106-107 2.281622672431528 1103 108-109 2.512383099450048 1103 110-111 2.99373858684228 1103 112-113 2.947767747252019 1103 114-115 3.048659600818997 1103 116-117 2.639925865584388 1103 118-119 2.7287654449825105 1103 120-121 2.6379570027797072 1104 1 0.8565444396237858 1104 2 0.7461003013918592 1104 3 0.7998546420868848 1104 4 1.0441352233029164 1104 5 1.4545119233207444 1104 6 1.0667133866504273 1104 7 0.816695934580423 1104 8 0.8747002533911044 1104 9 1.0243508920046338 1104 10-11 1.2882743071676828 1104 12-13 1.2084098421685425 1104 14-15 1.3112902498149523 1104 16-17 1.192206642134991 1104 18-19 1.1656198282165562 1104 20-21 1.2602298421150593 1104 22-23 1.2740145335833475 1104 24-25 1.4943924721912794 1104 26-27 1.3205188114762834 1104 28-29 1.1575313680182262 1104 30-31 1.2562130442464081 1104 32-33 1.422515130963049 1104 34-35 1.4099437227667622 1104 36-37 1.5732457056283877 1104 38-39 1.3484164855724217 1104 40-41 1.4962483227111747 1104 42-43 1.3515398602124762 1104 44-45 1.1530711514556629 1104 46-47 1.2736893130589664 1104 48-49 0.9466870282505369 1104 50-51 0.9017839804904284 1104 52-53 1.0285439626785333 1104 54-55 0.838449201016644 1104 56-57 1.2489865841779633 1104 58-59 1.1688914653927753 1104 60-61 1.2622640193713934 1104 62-63 1.2575044822640677 1104 64-65 1.0490538791037771 1104 66-67 1.163910654798869 1104 68-69 1.0843829646630638 1104 70-71 1.0020271101293652 1104 72-73 0.6425337733441019 1104 74-75 0.3002585858481517 1104 76-77 0.5463016483472352 1104 78-79 0.8759917737130358 1104 80-81 1.0304707857735522 1104 82-83 1.0151735652946599 1104 84-85 1.0990663326430585 1104 86-87 0.8387541462364112 1104 88-89 0.9126864155229022 1104 90-91 1.2024125356546982 1104 92-93 1.089612862692288 1104 94-95 0.9623954616244355 1104 96-97 0.6910446949580411 1104 98-99 0.8695143433581549 1104 100-101 0.8675771245818069 1104 102-103 1.3171849728463805 1104 104-105 1.491722236663616 1104 106-107 1.1068741819542076 1104 108-109 1.2816024539283362 1104 110-111 1.0992701669412561 1104 112-113 0.8029717015729538 1104 114-115 0.8267510380894265 1104 116-117 0.9096881594070965 1104 118-119 1.0026669966368154 1104 120-121 0.7819697726199806 1105 1 0.551486533619407 1105 2 0.468288517984373 1105 3 0.5046603988847131 1105 4 0.9428887900874372 1105 5 0.7472303536614788 1105 6 0.7618564691440213 1105 7 0.7940451399145374 1105 8 0.7928126668670785 1105 9 1.0181476560898162 1105 10-11 0.9231528157112123 1105 12-13 1.0910112423884115 1105 14-15 1.2434855619105036 1105 16-17 1.0328821462049973 1105 18-19 0.8428551400684796 1105 20-21 0.9317263232937094 1105 22-23 0.8102822989871399 1105 24-25 1.1816086901358958 1105 26-27 1.2460662767372597 1105 28-29 1.1313906821602018 1105 30-31 1.0494613533545376 1105 32-33 0.8664728381106244 1105 34-35 1.0120154269360526 1105 36-37 1.1411021632974752 1105 38-39 0.840647975597598 1105 40-41 0.7904269649425046 1105 42-43 0.9513062872155942 1105 44-45 1.0252637837045668 1105 46-47 1.181906088289871 1105 48-49 1.310196043802776 1105 50-51 1.2408135105732256 1105 52-53 1.310483623495216 1105 54-55 1.377900806945199 1105 56-57 1.6090209349394442 1105 58-59 1.2212352653544016 1105 60-61 1.2210202660025544 1105 62-63 1.475564821447385 1105 64-65 2.220254968096004 1105 66-67 1.9917670515623485 1105 68-69 1.1519699150280402 1105 70-71 1.2141750376638747 1105 72-73 1.3091303985645055 1105 74-75 0.9128441201578035 1105 76-77 1.3979919311624123 1105 78-79 1.6290454285061529 1105 80-81 1.7859667553451146 1105 82-83 1.817761748998148 1105 84-85 1.8188929343843512 1105 86-87 1.7748063057969183 1105 88-89 1.9866380016571306 1105 90-91 1.5715598876312384 1105 92-93 1.671456292652362 1105 94-95 1.7038465985798261 1105 96-97 1.2237433918826142 1105 98-99 1.4699080980973704 1105 100-101 1.5924193029316882 1105 102-103 1.5849336275938235 1105 104-105 1.7383351236806739 1105 106-107 1.2810033079283123 1105 108-109 1.5855077213496216 1105 110-111 1.463202476451361 1105 112-113 1.2652604733200583 1105 114-115 1.1051261983472997 1105 116-117 1.1414598730167604 1105 118-119 1.6113058477134743 1105 120-121 0.9676785768297762 1106 1 0.42938220738325583 1106 2 0.6003944838335755 1106 3 0.6691838570628654 1106 4 1.0967313403884233 1106 5 0.7698804368132883 1106 6 1.1333225174423376 1106 7 0.7014538486021422 1106 8 0.6773370619669805 1106 9 0.7522024191657266 1106 10-11 1.194977236576456 1106 12-13 1.221629844969634 1106 14-15 1.1454601244851617 1106 16-17 1.2281249260518194 1106 18-19 1.1811658701647545 1106 20-21 0.9308596706808672 1106 22-23 0.8765343777822565 1106 24-25 0.9231236925953112 1106 26-27 1.443897302900794 1106 28-29 1.2615993814785895 1106 30-31 1.199029198200293 1106 32-33 1.3718835611799776 1106 34-35 1.3227806577701458 1106 36-37 1.415678791784572 1106 38-39 1.514891996865721 1106 40-41 1.4899912963317306 1106 42-43 1.0696746379990927 1106 44-45 1.3166698690112995 1106 46-47 1.2662758913020795 1106 48-49 1.5923266038477522 1106 50-51 1.3127339659171966 1106 52-53 1.7274723570112442 1106 54-55 1.9897458400888013 1106 56-57 1.548964251173949 1106 58-59 1.9674231650100822 1106 60-61 1.6614437546306675 1106 62-63 2.169167988242883 1106 64-65 2.0063088879423496 1106 66-67 1.923594283192827 1106 68-69 2.2293128049631576 1106 70-71 2.2047823971841716 1106 72-73 2.472869184782386 1106 74-75 2.3413569911202536 1106 76-77 2.181469316213825 1106 78-79 1.910730950722911 1106 80-81 1.6253198108811482 1106 82-83 2.0343780561210956 1106 84-85 2.0331669371482697 1106 86-87 1.830623418672566 1106 88-89 1.8198776428161025 1106 90-91 1.9569860699093624 1106 92-93 2.173681481793441 1106 94-95 2.47309448465014 1106 96-97 2.187800731493322 1106 98-99 2.31827902541567 1106 100-101 1.886191115641033 1106 102-103 1.9454142684482925 1106 104-105 2.062580316100977 1106 106-107 2.0028780883372796 1106 108-109 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-0.15331742767063616 2316 76-77 -0.3473128474937397 2316 78-79 -0.15988705694913108 2316 80-81 -0.23920220819484328 2316 82-83 -0.5811790477237331 2316 84-85 -0.26782450187983287 2316 86-87 -0.25643181521254377 2316 88-89 -0.5636322755783745 2316 90-91 -0.5060151553747332 2316 92-93 -0.4798474164919675 2316 94-95 -0.3519741873180813 2316 96-97 -0.3732632635735058 2316 98-99 -0.18214942359890074 2316 100-101 0.09426517725760775 2316 102-103 -0.39541892473816276 2316 104-105 -0.5534773122935803 2316 106-107 -0.46366685075889436 2316 108-109 -0.37915650992264815 2316 110-111 -0.2306111063488281 2316 112-113 -0.10572805197775992 2316 114-115 0.05613160389201255 2316 116-117 -0.5881050418410787 2316 118-119 -1.1366018392417025 2316 120-121 -1.2042100135407523 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9605.0 3 1286.0 4 1070.0 5 1237.0 6 1472.0 7 1607.0 8 2020.0 9 2350.0 10 3316.0 11 4010.0 12 4285.0 13 5005.0 14 6157.0 15 6233.0 16 5085.0 17 4805.0 18 5161.0 19 5526.0 20 5964.0 21 6608.0 22 7211.0 23 8150.0 24 9275.0 25 10397.0 26 11770.0 27 13579.0 28 15912.0 29 18393.0 30 21507.0 31 25652.0 32 30797.0 33 37564.0 34 46913.0 35 59670.0 36 77861.0 37 84803.0 38 31422.0 39 165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.57112381197673 18.58933409696676 13.898580217940529 21.94096187311598 2 14.040719705033336 21.282560010899463 38.60589081805564 26.070829466011563 3 21.718801538372603 25.169768462001446 30.954987979338743 22.156442020287205 4 11.081771315087872 16.170969584852276 42.182994178138095 30.564264921921758 5 10.740235029255379 37.79223495443779 36.330231237363904 15.13729877894293 6 26.606888132872925 36.657366650174865 19.256984788437375 17.478760428514835 7 23.975494505307655 32.714971442562906 24.661077037916183 18.648457014213253 8 22.49860128800543 30.46386221368918 22.224300686516756 24.813235811788633 9 21.590860773745643 19.549603636914064 26.42001051072247 32.43952507861783 10-11 23.512048018586963 24.213311244874042 29.23424028683248 23.040400449706517 12-13 26.73679000334231 23.056675565322628 28.32366501025231 21.882869421082756 14-15 22.332396675659034 24.979758668902853 30.31743169826469 22.370412957173425 16-17 19.385590697706064 28.67801897608088 30.16637239793297 21.770017928280076 18-19 18.344736092389986 30.67527861993307 30.27362369619478 20.70636159148216 20-21 20.32448688682423 27.291446242613315 28.416927206480214 23.96713966408225 22-23 18.923428901911088 27.15760735518001 29.94233566088606 23.976628082022845 24-25 22.015707095752383 26.514846718445245 28.986251825590504 22.48319436021187 26-27 20.82237085541778 24.85868930019624 32.95837618375622 21.360563660629754 28-29 18.90309980588999 29.122030720758886 30.77034965320486 21.204519820146274 30-31 19.708992908573684 26.988678898583075 29.817082715158776 23.485245477684465 32-33 20.317733648577022 26.77084228428594 30.912116604999035 21.999307462138002 34-35 22.2717187117149 25.739072581742946 29.938607715553623 22.05060099098854 36-37 21.211136397473254 25.366569538677496 32.347363719505715 21.074930344343535 38-39 20.92325833061091 27.58238729173473 30.96082775236851 20.533526625285855 40-41 19.763090306932806 28.707520147277382 30.642407413551524 20.88698213223828 42-43 19.095963875305834 28.51421201819846 28.18022193411395 24.209602172381757 44-45 20.006398154064485 26.840086136850733 28.37564311232759 24.77787259675719 46-47 21.876369379004966 27.845691885788582 29.346297284258192 20.93164145094826 48-49 21.27581726486112 26.768354476694366 29.774146529490796 22.181681728953713 50-51 18.179232396453234 27.664930525985902 32.51337872960829 21.64245834795257 52-53 19.65909506752484 28.96347301261479 28.662974905727335 22.71445701413303 54-55 18.762184770260436 27.274142970807606 29.992979530011443 23.970692728920508 56-57 21.14796326354223 25.712628072288744 29.02328184920902 24.11612681496 58-59 22.94240231400827 25.71426385101786 28.79280630468626 22.55052753028761 60-61 21.434933327662108 26.308755662363627 31.73016311860658 20.52614789136769 62-63 19.376239730983695 27.43063891371898 31.03852211296982 22.154599242327503 64-65 18.62340079048557 29.64627345556945 29.821208610384936 21.909117143560046 66-67 19.746601820251254 27.054507935399897 28.133787019877023 25.06510322447183 68-69 18.80768513527667 27.25612263926817 29.45696036431417 24.479231861140985 70-71 21.741828360626116 26.244284881401704 28.40443776188966 23.60944899608252 72-73 20.16193648693802 25.0251454172264 32.18204259207484 22.63087550376073 74-75 18.633962881761054 28.96397287224793 30.125921051285637 22.276143194705384 76-77 19.218351538954575 27.031512089350734 29.220277789142084 24.529858582552606 78-79 20.117465066467208 27.119640975079047 29.543909155815456 23.21898480263829 80-81 21.64174602270941 26.015554832356024 28.581175984752765 23.761523160181806 82-83 19.99904727960047 24.86413101266523 31.77399090322147 23.362830804512832 84-85 20.43452630576082 26.4993675456945 30.076243280970484 22.98986286757419 86-87 20.250385856959806 27.40265088411018 29.486618803954034 22.86034445497598 88-89 19.069446477133102 26.820570506689723 27.833048000571797 26.276935015605375 90-91 19.424962430583804 25.111687109307855 28.922323636481224 26.541026823627124 92-93 20.977222036426703 26.27035205556506 29.360486143199843 23.3919397648084 94-95 19.877252164085878 25.819680501611792 29.363147736517654 24.939919597784684 96-97 17.12001797180673 26.848279488824634 31.850347267723762 24.18135527164488 98-99 19.05387849071822 27.54739583466271 28.903397832336626 24.495327842282453 100-101 18.070211116660868 25.364461504032835 30.282319926278074 26.283007453028222 102-103 20.315417041778208 24.38278905996055 29.358852830907946 25.9429410673533 104-105 21.972679353440867 25.185136016039735 28.759354666312646 24.082829964206752 106-107 20.61304638269446 25.329606038557927 30.944120362687904 23.113227216059702 108-109 20.21364233631307 25.992129683212937 29.722785792580385 24.071442187893602 110-111 19.932104092307114 27.862683122360714 27.688049877705666 24.517162907626503 112-113 20.600143985540175 25.742180785248802 27.02332872103057 26.634346508180457 114-115 19.457419025988777 25.155957353928528 28.608420463238655 26.77820315684404 116-117 21.868049776997285 24.434495397856928 27.562234009063047 26.135220816082743 118-119 21.153584337964507 23.48267223950498 30.099283200479228 25.264460222051287 120-121 20.184957934707313 27.06313963838996 27.898403340033596 24.853499086869128 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1828.0 1 1665.5 2 1061.5 3 586.0 4 539.5 5 473.5 6 430.5 7 448.5 8 530.0 9 582.5 10 528.5 11 508.5 12 524.5 13 559.5 14 601.0 15 661.5 16 728.0 17 761.0 18 821.5 19 930.0 20 1029.5 21 1167.0 22 1323.0 23 1520.0 24 1892.0 25 2405.0 26 2997.5 27 3886.5 28 5101.5 29 6534.5 30 8107.0 31 9599.0 32 10941.0 33 12023.0 34 13155.0 35 14262.5 36 15123.5 37 16092.0 38 17098.0 39 17564.0 40 17985.0 41 18323.0 42 18511.5 43 19161.0 44 19392.5 45 19769.0 46 22154.0 47 31700.5 48 51568.5 49 51674.5 50 32799.0 51 22269.5 52 16440.0 53 13318.0 54 11683.0 55 10306.5 56 8889.0 57 7344.0 58 5886.5 59 4845.5 60 3919.0 61 3029.5 62 2251.0 63 1631.0 64 1136.0 65 750.0 66 479.5 67 295.0 68 180.0 69 112.5 70 69.5 71 51.5 72 41.5 73 42.0 74 30.0 75 21.0 76 20.5 77 18.0 78 14.5 79 12.0 80 10.5 81 7.0 82 4.5 83 3.5 84 4.5 85 3.0 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 1.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.620029199636941 2 1.1211717575184013 3 0.9579973157888533 4 0.9623755773832479 5 0.9675958123611796 6 0.9551346062848262 7 0.9649014975338599 8 0.9770259142567985 9 0.9888135416263221 10-11 0.9942021712809614 12-13 0.9979910515068798 14-15 0.9997591956123084 16-17 1.0011063530259683 18-19 0.9936127899124853 20-21 1.0038006678532878 22-23 1.0032954838231654 24-25 1.0136517564406753 26-27 1.0175248340049474 28-29 1.015756689899519 30-31 1.0256919758252603 32-33 1.0355430644126478 34-35 1.0351220777208792 36-37 1.0323435655552056 38-39 1.0324277628935594 40-41 1.0286388826676411 42-43 1.027375922592335 44-45 1.0395845366536272 46-47 1.0463203237219265 48-49 1.0486778491958313 50-51 1.1311070434441426 52-53 1.064001764776212 54-55 1.0567607936777903 56-57 1.0253551864718453 58-59 0.9720582712939279 60-61 1.0239238317198318 62-63 1.0975965027793542 64-65 1.0916184917562386 66-67 1.0956599639972182 68-69 1.1210875601800476 70-71 1.2193458540388622 72-73 1.221703379512767 74-75 1.2267552198139913 76-77 1.2142940137376377 78-79 0.9401474800578603 80-81 0.9107626089724051 82-83 1.0192087807720223 84-85 1.0193771754487297 86-87 1.0423630488193008 88-89 1.0482568625040625 90-91 1.0548242548956543 92-93 1.0574343723846202 94-95 1.060381279227001 96-97 1.0534770974819945 98-99 1.0372270111797226 100-101 1.049014638549246 102-103 1.0507827826546747 104-105 1.0612232526105385 106-107 1.058444740444865 108-109 1.0640859621145657 110-111 1.065264724851518 112-113 1.0581921484298038 114-115 1.054571662880593 116-117 1.0410158914056409 118-119 1.0492672305643076 120-121 1.0615600419639535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 593843.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.16574973769458 #Duplication Level Percentage of deduplicated Percentage of total 1 89.63094137733847 60.2012937730429 2 6.856505901250361 9.210447188768155 3 1.4235004248766705 2.8683141986630507 4 0.654096103949819 1.7573142088917841 5 0.35402885546988955 1.188930675320652 6 0.2526322519107013 1.0180940764502626 7 0.15071008081423598 0.708578889964169 8 0.11279901423896722 0.6060984288826496 9 0.08301244154393957 0.5018033586469721 >10 0.431419858677796 5.014369487747835 >50 0.02643569315368527 1.243915680504466 >100 0.01535787161366879 2.1854758609729235 >500 0.0027694522582021484 1.230387060077202 >1k 0.004783599355076438 5.776021276945711 >5k 5.035367742185725E-4 2.5769472798950632 >10k+ 5.035367742185725E-4 3.912008555226222 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12886 2.169933804052586 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10248 1.725708646898254 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8255 1.3900980562202467 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6984 1.17606842212504 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4199 0.7070892474947083 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2860 0.4816087753833926 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2768 0.4661164651263044 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2233 0.3760253130878027 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1935 0.3258436994289736 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1884 0.31725557091689216 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1822 0.30681510096102843 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1777 0.2992373405091918 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1658 0.2791983739810017 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1562 0.26303248501708365 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1503 0.2530971990913423 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1455 0.24501425460938328 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1390 0.2340686006233971 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1388 0.23373181126998213 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1321 0.22244936793058095 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1309 0.2204286318100912 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1065 0.1793403306934661 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1023 0.17226775427175195 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1005 0.16923665009101732 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 842 0.1417883177876981 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 829 0.13959918699050086 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 796 0.13404216265915403 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 695 0.11703430031169855 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 691 0.11636072160486861 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 634 0.10676222503254228 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 615 0.10356272617510016 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.525920150612199E-4 0.0 12-13 0.0 0.0 0.0 8.419733835373996E-4 0.0 14-15 0.0 0.0 0.0 0.0010945653985986194 0.0 16-17 0.0 0.0 0.0 0.0011787627369523595 0.0 18-19 0.0 0.0 0.0 0.0012629600753060995 0.0 20-21 0.0 0.0 0.0 0.0019365387821360192 0.0 22-23 0.0 0.0 0.0 0.0031153015190883786 0.0 24-25 0.0 0.0 0.0 0.005220234977931878 0.0 26-27 0.0 0.0 0.0 0.007325168436775376 0.0 28-29 0.0 0.0 0.0 0.014060955505074574 0.0 30-31 0.0 0.0 0.0 0.027532529641672965 0.0 32-33 0.0 0.0 0.0 0.049423837613645355 0.0 34-35 0.0 0.0 0.0 0.07064156687878784 0.0 36-37 0.0 0.0 0.0 0.09051213873027046 0.0 38-39 0.0 0.0 0.0 0.1318530318619568 0.0 40-41 0.0 0.0 0.0 0.21251408200483968 0.0 42-43 0.0 0.0 0.0 0.3360315773697762 0.0 44-45 0.0 0.0 0.0 0.4372367780709716 0.0 46-47 0.0 0.0 0.0 0.7043949326673885 0.0 48-49 8.419733835373996E-5 0.0 0.0 0.8971226401590993 0.0 50-51 1.6839467670747992E-4 0.0 0.0 1.017945820696716 0.0 52-53 1.6839467670747992E-4 0.0 0.0 1.1976229407435972 0.0 54-55 1.6839467670747992E-4 0.0 0.0 1.348588768411853 0.0 56-57 1.6839467670747992E-4 0.0 0.0 1.7975289765139943 0.0 58-59 1.6839467670747992E-4 0.0 0.0 2.3502845028062973 0.0 60-61 1.6839467670747992E-4 0.0 0.0 2.8212844135571187 0.0 62-63 1.6839467670747992E-4 0.0 0.0 3.2230741121811657 0.0 64-65 1.6839467670747992E-4 0.0 0.0 3.8179283076503383 0.0 66-67 1.6839467670747992E-4 0.0 0.0 4.696106546679847 0.0 68-69 1.6839467670747992E-4 0.0 0.0 5.515515043538444 0.0 70-71 1.6839467670747992E-4 0.0 0.0 5.922945963832191 0.0 72-73 1.6839467670747992E-4 0.0 0.0 6.19894483895575 0.0 74-75 1.6839467670747992E-4 0.0 0.0 6.580358781698193 0.0 76-77 3.3678935341495983E-4 0.0 0.0 6.9474591769205 0.0 78-79 3.3678935341495983E-4 0.0 0.0 7.351943190371866 0.0 80-81 3.3678935341495983E-4 0.0 0.0 8.147523840476355 0.0 82-83 3.3678935341495983E-4 0.0 0.0 9.012819886737741 0.0 84-85 3.3678935341495983E-4 0.0 0.0 10.044995057616239 0.0 86-87 3.3678935341495983E-4 0.0 0.0 10.961062098904929 0.0 88-89 3.3678935341495983E-4 0.0 0.0 12.115828594426473 0.0 90-91 3.3678935341495983E-4 0.0 0.0 13.213340899867474 0.0 92-93 3.3678935341495983E-4 0.0 0.0 14.209816399283987 0.0 94-95 3.3678935341495983E-4 0.0 0.0 15.065766540988106 0.0 96-97 3.3678935341495983E-4 0.0 0.0 16.075376825187803 0.0 98-99 3.3678935341495983E-4 0.0 0.0 17.031605996871228 0.0 100-101 3.3678935341495983E-4 0.0 0.0 17.569037607583148 0.0 102-103 3.3678935341495983E-4 0.0 0.0 17.98918232596831 0.0 104-105 3.3678935341495983E-4 0.0 0.0 18.409158649676765 0.0 106-107 3.3678935341495983E-4 0.0 0.0 19.021862680876932 0.0 108-109 3.3678935341495983E-4 0.0 0.0 19.815422594860934 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 725 0.0 57.12988 2 GTACCCG 135 0.0 55.48119 1 ACCTGGG 780 0.0 53.02008 3 TATAACG 105 8.589268E-9 43.829803 2 GTACCGG 70 6.587905E-5 41.153625 1 GTACCTG 1065 0.0 39.492027 1 GTACGCA 160 2.4738256E-10 35.899014 3 TAACGCA 130 5.723814E-8 35.34672 4 TACCGGG 85 2.0841556E-4 33.839184 2 GAGTACG 190 4.1836756E-11 33.3561 1 TACCCGG 190 4.1836756E-11 33.304882 2 AGTACGC 175 6.384653E-10 32.87235 2 CCTGGGG 580 0.0 32.68048 4 GTATAAC 150 1.9577419E-7 30.728043 1 TACGCAG 195 2.068191E-9 29.4556 4 GACAACG 80 0.0063653733 28.721657 7 ACAACGC 80 0.0063680224 28.719212 3 ATAACGC 185 4.0345185E-8 27.943014 3 GGATGGC 330 0.0 26.103926 8 GGGATGG 350 0.0 24.618563 7 >>END_MODULE