##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003799_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 593843 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.57408607999084 31.0 31.0 34.0 30.0 34.0 2 31.77232366130442 31.0 31.0 34.0 30.0 34.0 3 31.841252654321092 31.0 31.0 34.0 30.0 34.0 4 35.55204658470336 37.0 35.0 37.0 33.0 37.0 5 35.44337476403696 37.0 35.0 37.0 33.0 37.0 6 35.494733456485974 37.0 35.0 37.0 33.0 37.0 7 35.42844825989361 37.0 35.0 37.0 33.0 37.0 8 35.429600079482285 37.0 35.0 37.0 33.0 37.0 9 36.969796730785745 39.0 37.0 39.0 33.0 39.0 10-11 36.88829875909963 39.0 37.0 39.0 33.0 39.0 12-13 36.838816825322525 39.0 37.0 39.0 32.0 39.0 14-15 37.83485786647313 39.0 37.0 41.0 32.5 41.0 16-17 37.918777353610295 40.0 37.0 41.0 33.0 41.0 18-19 37.96203289421615 40.0 37.0 41.0 33.0 41.0 20-21 37.867395927879926 40.0 37.0 41.0 32.5 41.0 22-23 37.77043679895191 39.5 37.0 41.0 32.0 41.0 24-25 37.62047460355683 39.0 36.5 41.0 32.0 41.0 26-27 37.31861451595792 39.0 36.0 41.0 31.5 41.0 28-29 37.190086436987556 39.0 36.0 40.0 31.0 41.0 30-31 36.99515107528421 39.0 36.0 40.0 31.0 41.0 32-33 36.714785726193625 39.0 35.0 40.0 30.0 41.0 34-35 36.59115288047514 38.0 35.0 40.0 30.0 41.0 36-37 36.35830345731111 38.5 35.0 40.0 30.0 41.0 38-39 36.10739791493711 38.0 35.0 40.0 30.0 41.0 40-41 36.37857565046654 39.0 35.0 40.0 30.0 41.0 42-43 36.2292558807631 39.0 35.0 40.0 30.0 41.0 44-45 36.04395016864727 39.0 35.0 40.0 29.5 41.0 46-47 35.75825933790581 39.0 35.0 40.0 28.5 41.0 48-49 35.42510562556097 38.0 35.0 40.0 27.0 41.0 50-51 35.11477191783013 38.0 34.0 40.0 26.0 41.0 52-53 35.016188622245274 38.0 34.0 40.0 26.0 41.0 54-55 34.80900423175822 38.0 34.0 40.0 26.0 41.0 56-57 34.48154646935301 38.0 33.5 40.0 24.5 41.0 58-59 34.163065827163074 38.0 33.0 40.0 23.0 41.0 60-61 33.61995931584611 37.0 32.0 40.0 22.0 41.0 62-63 33.47338690529315 37.0 32.0 40.0 22.0 41.0 64-65 33.01659276946937 36.0 32.0 39.0 21.0 41.0 66-67 32.61401498375834 36.0 31.0 39.0 20.0 40.0 68-69 32.24784917899176 35.5 31.0 39.0 19.5 40.0 70-71 31.737338825245054 35.0 31.0 38.0 18.0 40.0 72-73 31.31296150666085 35.0 30.5 37.0 16.0 40.0 74-75 30.981163371463502 34.5 30.0 37.0 13.0 39.0 76-77 30.043172185240877 34.0 29.0 36.0 12.5 39.0 78-79 30.407652359293618 34.0 30.0 36.0 11.0 39.0 80-81 30.113374242013464 34.0 29.5 35.5 7.0 37.5 82-83 29.79629969537403 34.0 29.5 35.0 7.0 37.0 84-85 29.467240499593327 34.0 29.0 35.0 2.0 37.0 86-87 29.214070890791 34.0 29.0 35.0 2.0 36.0 88-89 28.896998028098334 34.0 29.0 35.0 2.0 36.0 90-91 28.52993131181137 34.0 29.0 35.0 2.0 35.5 92-93 28.239750237015507 34.0 29.0 35.0 2.0 35.0 94-95 27.835432934294083 33.5 27.0 35.0 2.0 35.0 96-97 27.38341278755496 33.0 27.0 35.0 2.0 35.0 98-99 27.046595649018343 33.0 26.5 35.0 2.0 35.0 100-101 26.008955228907304 32.5 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08805983694397312 1101 2 0.2068335762415252 1101 3 0.10139191755478194 1101 4 0.27342025385391366 1101 5 0.2446847452200771 1101 6 0.3971277645908273 1101 7 0.335920737438407 1101 8 0.2823523847880125 1101 9 0.3755231872650526 1101 10-11 0.42483434971217804 1101 12-13 0.3891502571983736 1101 14-15 0.364105788896552 1101 16-17 0.44935573864916734 1101 18-19 0.2553969899537165 1101 20-21 0.19617893404591769 1101 22-23 0.16214019708464633 1101 24-25 0.3143357347467344 1101 26-27 0.4024569879194999 1101 28-29 0.3310173719858369 1101 30-31 0.39232188134115376 1101 32-33 0.43456046988634967 1101 34-35 0.25046638638760044 1101 36-37 0.3775238162922605 1101 38-39 0.5127938051855168 1101 40-41 0.4610586670675403 1101 42-43 0.28972452568581986 1101 44-45 0.5173983819288637 1101 46-47 0.4882527626311557 1101 48-49 0.4157827663641669 1101 50-51 0.40608260060238166 1101 52-53 0.6157683110130208 1101 54-55 0.6725191137090576 1101 56-57 0.6220832299648436 1101 58-59 0.5867134481388305 1101 60-61 0.52367925369186 1101 62-63 0.38982043305133374 1101 64-65 0.39703587574227583 1101 66-67 0.5015888504526842 1101 68-69 0.5794738020320125 1101 70-71 0.5903676235137887 1101 72-73 0.6169850728264592 1101 74-75 0.38054167342765766 1101 76-77 0.4729097453655662 1101 78-79 0.6642300507300973 1101 80-81 0.7837562478498477 1101 82-83 0.8376861017739508 1101 84-85 0.7811465157356032 1101 86-87 0.6167951989340388 1101 88-89 0.5094594614177304 1101 90-91 0.5398809677059937 1101 92-93 0.6788201750413521 1101 94-95 0.6352914898492124 1101 96-97 0.7801720478975902 1101 98-99 0.6721782649253178 1101 100-101 1.0298158270192594 1102 1 0.28481373084756 1102 2 0.19101877513671184 1102 3 0.21678624351319087 1102 4 0.12138289515918643 1102 5 0.16627388183811576 1102 6 0.22501643517433934 1102 7 0.08042877518941793 1102 8 0.1682621961911437 1102 9 0.22873971140045057 1102 10-11 0.2635547637963569 1102 12-13 0.1993242370952899 1102 14-15 0.328162598917082 1102 16-17 0.19167154743896475 1102 18-19 0.1189675168290023 1102 20-21 0.04853742311957632 1102 22-23 0.020066106136724216 1102 24-25 0.23729418395593171 1102 26-27 0.08689814196400647 1102 28-29 0.34362340037819195 1102 30-31 0.2990172124534638 1102 32-33 0.11300645196000403 1102 34-35 0.27916152134145733 1102 36-37 0.17593619832035046 1102 38-39 0.1935281971417524 1102 40-41 0.3646227396967845 1102 42-43 0.5792803533321376 1102 44-45 0.3528976843007925 1102 46-47 0.4737465282724216 1102 48-49 0.33310703844227874 1102 50-51 0.3558805890290344 1102 52-53 0.44953382572332856 1102 54-55 0.5141141983577597 1102 56-57 0.7184138012605317 1102 58-59 0.5435873783633696 1102 60-61 0.5437158910296702 1102 62-63 0.2253137241156793 1102 64-65 0.12616287568849316 1102 66-67 0.27633503089948164 1102 68-69 0.5819219861425466 1102 70-71 0.47411241503214896 1102 72-73 0.2825019978211607 1102 74-75 0.415479709501831 1102 76-77 0.47443883218467064 1102 78-79 0.695948240630468 1102 80-81 0.7329179868262194 1102 82-83 0.6309648271236341 1102 84-85 0.5199191332803927 1102 86-87 0.4704293507543582 1102 88-89 0.512086086448182 1102 90-91 0.6273806117733081 1102 92-93 0.7156163379288003 1102 94-95 0.7666635549864758 1102 96-97 0.8625785325165509 1102 98-99 0.726029140203341 1102 100-101 0.85767412468023 1103 1 0.027523438476542594 1103 2 0.011048135628499267 1103 3 0.020165863914936466 1103 4 0.011542859217897217 1103 5 0.0764855115035985 1103 6 0.05076761133412333 1103 7 -0.008332136124813871 1103 8 0.16127958785314433 1103 9 0.07475690374014476 1103 10-11 0.04150101637884518 1103 12-13 -0.020811428625385986 1103 14-15 -0.009840571256710007 1103 16-17 0.05424079612043187 1103 18-19 0.15712112788474997 1103 20-21 0.0024049876857645813 1103 22-23 0.021335599383505155 1103 24-25 -0.03135392042567986 1103 26-27 0.1797558384942164 1103 28-29 0.10841200800116013 1103 30-31 -0.09915321444429281 1103 32-33 0.21080568061432103 1103 34-35 0.21770776569794492 1103 36-37 0.1653590653344068 1103 38-39 0.14363671706291115 1103 40-41 0.4534916172591039 1103 42-43 0.37813448737163924 1103 44-45 0.41816466545376585 1103 46-47 0.5529079350449493 1103 48-49 0.48465249816493383 1103 50-51 0.5965998419816927 1103 52-53 0.4279330619708759 1103 54-55 0.6396647075787882 1103 56-57 0.6462211609993247 1103 58-59 0.6483992718936165 1103 60-61 0.9489666100136915 1103 62-63 0.7210464866911721 1103 64-65 0.593012887236533 1103 66-67 0.4241942144373638 1103 68-69 0.32679180969484634 1103 70-71 0.5351952097206834 1103 72-73 0.5646862222517477 1103 74-75 0.7551585316652023 1103 76-77 0.7187909714192067 1103 78-79 0.6453603189293311 1103 80-81 0.6391202325241743 1103 82-83 0.6035864849157129 1103 84-85 0.31984383374326697 1103 86-87 0.6551093751792507 1103 88-89 0.6079268943679246 1103 90-91 0.6307813508477551 1103 92-93 0.5402186424743007 1103 94-95 0.2876861258645391 1103 96-97 0.5595781781657898 1103 98-99 0.5958372687567355 1103 100-101 0.8870955205058486 1104 1 -0.14324698837969407 1104 2 -0.12375677864178414 1104 3 -0.09560533108699332 1104 4 -0.014014279183335532 1104 5 0.0774375466241537 1104 6 -0.03832797857849357 1104 7 -0.010414276504604913 1104 8 -0.0743688900545223 1104 9 -0.31489081998810775 1104 10-11 -0.3234298737856349 1104 12-13 -0.1451020577134372 1104 14-15 -0.013290243747320574 1104 16-17 -0.014732286192206345 1104 18-19 -0.10743174148858259 1104 20-21 -0.2688639191730786 1104 22-23 -0.09761176168687768 1104 24-25 -0.16802391572370112 1104 26-27 -0.16526785763665686 1104 28-29 -0.10482756500294244 1104 30-31 -0.30056508951121685 1104 32-33 -0.006246688300450387 1104 34-35 0.06309283946320932 1104 36-37 0.09575633632223912 1104 38-39 0.168141728631646 1104 40-41 0.3797804726289087 1104 42-43 0.2623766767265394 1104 44-45 0.2636253899315406 1104 46-47 0.22464378137856755 1104 48-49 0.5902742771903675 1104 50-51 1.0354094722010032 1104 52-53 0.90405711517311 1104 54-55 0.8842475346342837 1104 56-57 0.8323847620663472 1104 58-59 1.0563009306227826 1104 60-61 0.8555531102022442 1104 62-63 1.008562256955095 1104 64-65 0.7954600132077729 1104 66-67 0.7115226037056672 1104 68-69 1.0918055664859523 1104 70-71 0.9510692896916346 1104 72-73 1.2850334706607534 1104 74-75 0.9385469154401065 1104 76-77 0.7683846689001577 1104 78-79 1.0328473419224942 1104 80-81 1.162412504978473 1104 82-83 1.0140695787272485 1104 84-85 0.7704055111872457 1104 86-87 0.9078549141149246 1104 88-89 0.5067341735958699 1104 90-91 0.5383390897992619 1104 92-93 0.8178806164690791 1104 94-95 0.7411591918831668 1104 96-97 0.4626108406518803 1104 98-99 0.4779984815889584 1104 100-101 0.5803644692635466 1105 1 -0.04259438481732403 1105 2 -0.11635370229965503 1105 3 -0.2984179519465542 1105 4 -0.3429136844400986 1105 5 -0.20627598995118746 1105 6 -0.03412853708289276 1105 7 -0.16472167318585917 1105 8 -0.09433070223990114 1105 9 -0.42642420996365615 1105 10-11 -0.47530104624899394 1105 12-13 -0.35365503188641156 1105 14-15 -0.14353688099657802 1105 16-17 -0.3296751719606945 1105 18-19 -0.22851969831869212 1105 20-21 -0.5129674952457037 1105 22-23 -0.5284656278224489 1105 24-25 -0.7248945248102032 1105 26-27 -0.45197795000000696 1105 28-29 -0.6871688982875455 1105 30-31 -0.4769979700113751 1105 32-33 -0.0027598423885848433 1105 34-35 -0.0892305571205938 1105 36-37 -0.3856628860958935 1105 38-39 -0.10162442429623297 1105 40-41 -0.3470045945347451 1105 42-43 -0.02097751677666082 1105 44-45 -0.024983434681637107 1105 46-47 -0.2841235805255877 1105 48-49 -0.28357194224643933 1105 50-51 -0.16435786838618327 1105 52-53 -0.07278352835551516 1105 54-55 -3.7288640616850444E-4 1105 56-57 -0.14260435105667568 1105 58-59 -0.04119828521754698 1105 60-61 0.24029838773385848 1105 62-63 -0.15252393361244998 1105 64-65 -0.24093260868718147 1105 66-67 -0.10143019140483034 1105 68-69 0.04766727421822736 1105 70-71 -0.11803268760884578 1105 72-73 -0.15253067055498448 1105 74-75 -0.3005806726674649 1105 76-77 -0.3848553570456339 1105 78-79 -0.3087310877482814 1105 80-81 0.016118763333960118 1105 82-83 0.09144405988762472 1105 84-85 -0.384661682921962 1105 86-87 -0.24612359229691094 1105 88-89 0.0455295216257241 1105 90-91 -0.07600357122754531 1105 92-93 -0.048570139826278336 1105 94-95 0.09063098801992808 1105 96-97 0.07598053463166465 1105 98-99 0.08204200473461043 1105 100-101 -0.15207568336590072 1106 1 -0.20554849907493278 1106 2 -0.21065487579413755 1106 3 0.026647046999407564 1106 4 -0.1930530515494624 1106 5 -0.11066312601564476 1106 6 -0.31679782713044347 1106 7 -0.12031158253867602 1106 8 -0.12192304758925587 1106 9 -0.39025903066025336 1106 10-11 -0.5064185596586839 1106 12-13 -0.32279517195620855 1106 14-15 -0.3502101061154477 1106 16-17 0.07033185495237859 1106 18-19 -0.24697705666783065 1106 20-21 -0.2006914781000333 1106 22-23 -0.30488096523139063 1106 24-25 -0.34336827928986224 1106 26-27 -0.2855689368128367 1106 28-29 0.05391277786545601 1106 30-31 -0.056179568868323315 1106 32-33 -0.09978980046133756 1106 34-35 -0.29630197398050484 1106 36-37 -0.30033273829649687 1106 38-39 -0.4844740717794238 1106 40-41 -0.022548594722131554 1106 42-43 -0.12136868160461489 1106 44-45 -0.10217407483340679 1106 46-47 -0.2607356715006901 1106 48-49 -0.02639863514691143 1106 50-51 0.17104840430489077 1106 52-53 0.23739612638882335 1106 54-55 0.03335629636384141 1106 56-57 0.23210578877889532 1106 58-59 -0.04691350785357429 1106 60-61 0.22445269874503282 1106 62-63 -0.04589052766768731 1106 64-65 0.18895051032529864 1106 66-67 -0.025317011258202626 1106 68-69 -9.823873187961851E-4 1106 70-71 0.37287917147812166 1106 72-73 0.3525169953387213 1106 74-75 0.618726942164006 1106 76-77 0.09351346219873946 1106 78-79 0.20818034972390365 1106 80-81 0.15198412767940184 1106 82-83 0.28346617124077156 1106 84-85 0.16164567425602883 1106 86-87 0.16216348052532936 1106 88-89 0.48745208504361415 1106 90-91 0.22522848086530445 1106 92-93 -0.006631180277874904 1106 94-95 -0.10289019812300282 1106 96-97 -0.3244363955442431 1106 98-99 -0.3073075373448866 1106 100-101 -0.31316766565807796 1107 1 -0.4610780940905066 1107 2 -0.37506297548261003 1107 3 -0.4868264732498133 1107 4 -0.3276881205303255 1107 5 -0.3092835087481802 1107 6 -0.41094557968941103 1107 7 -0.6770650315818472 1107 8 -0.42647355938276377 1107 9 -0.5726021548258089 1107 10-11 -0.5999877630409642 1107 12-13 -0.3459482649691168 1107 14-15 -0.47903186846524193 1107 16-17 -0.7063982296048081 1107 18-19 -0.586947016614431 1107 20-21 -0.4427920566492105 1107 22-23 -0.367970640353775 1107 24-25 -0.4723569308252422 1107 26-27 -0.4249102808270706 1107 28-29 -0.6397064922725093 1107 30-31 -0.7588275439462038 1107 32-33 -0.6514753812357057 1107 34-35 -0.6786729130103168 1107 36-37 -0.5198420840908824 1107 38-39 -0.08520838419597965 1107 40-41 -0.01088304065003598 1107 42-43 -0.44152889522278116 1107 44-45 0.08137621444117116 1107 46-47 -0.5158591694980217 1107 48-49 -0.2604829698153637 1107 50-51 -0.10480273054157152 1107 52-53 -0.38697525767882013 1107 54-55 -0.3311686257545432 1107 56-57 -0.2806056041895104 1107 58-59 0.21597590024611435 1107 60-61 -0.3765875771784195 1107 62-63 -0.22670939727159833 1107 64-65 -0.24074463876237218 1107 66-67 -0.5408098906529517 1107 68-69 -0.9107287157567114 1107 70-71 -0.8385527377341582 1107 72-73 -0.5150619988757832 1107 74-75 -0.7899290435947819 1107 76-77 -0.8273365600531513 1107 78-79 -0.90039775441495 1107 80-81 -0.5664626903001242 1107 82-83 -0.3792952884833021 1107 84-85 -0.08484965284677415 1107 86-87 -0.12857973264778977 1107 88-89 -0.633041906945703 1107 90-91 -0.6672003130821835 1107 92-93 -0.7875112425831716 1107 94-95 -0.25576625508784545 1107 96-97 -0.5261497911828705 1107 98-99 -0.7737850629345608 1107 100-101 -0.9640744302330688 1108 1 -0.44427481540198244 1108 2 -0.5098990410563822 1108 3 -0.31346581751211033 1108 4 -0.4365288699448442 1108 5 -0.3074099724484114 1108 6 -0.41434136423273316 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-0.9534229176282629 2316 86-87 -0.882086893867033 2316 88-89 -0.7010734897587803 2316 90-91 -1.0583727332070438 2316 92-93 -1.0556025022232731 2316 94-95 -1.0046232143239813 2316 96-97 -0.5087976060928945 2316 98-99 -0.7934049674516395 2316 100-101 -0.8441561415808465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 6.0 5 13.0 6 46.0 7 116.0 8 270.0 9 458.0 10 768.0 11 1345.0 12 2052.0 13 2814.0 14 3179.0 15 3823.0 16 4835.0 17 6080.0 18 5396.0 19 4219.0 20 3934.0 21 3995.0 22 4330.0 23 4640.0 24 5254.0 25 6254.0 26 7941.0 27 9642.0 28 11945.0 29 14617.0 30 18196.0 31 21906.0 32 27229.0 33 33572.0 34 42685.0 35 56596.0 36 78957.0 37 100677.0 38 87703.0 39 18342.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.48564937625768 19.588980608540602 13.669580048335394 19.25578996686632 2 15.030640236971374 25.11489036369178 37.580810572776244 22.273658826560595 3 24.555733151881913 26.68565394092611 29.00404391887909 19.75456898831289 4 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28.349664810396014 22.3519516101057 24-25 23.83778204003415 28.242734190686765 28.439924357111224 19.479559412167863 26-27 22.864196782380393 26.159200260023276 31.962316834317683 19.01428612327865 28-29 20.307202872458852 31.4794948271826 29.287762914360105 18.925539385998448 30-31 22.247409190080862 28.01939770774266 28.13897031146979 21.59422279070668 32-33 22.201723777896035 28.835136373128194 29.067280553441154 19.895859295534613 34-35 23.857948804311217 26.722802290333448 29.974149545301447 19.44509936005389 36-37 23.24920017213458 26.420047109629685 31.395949110820474 18.93480360741526 38-39 21.070536008999724 29.138879345768583 30.86901898968669 18.921565655545002 40-41 20.60779063305209 29.877650347766043 28.760083531214743 20.754475487967124 42-43 20.200878009844352 29.985955883962596 27.07929536931479 22.733870736878263 44-45 22.705243641837995 26.839248757668273 27.772239463966063 22.68326813652767 46-47 24.218290203210447 29.345491439228415 27.548129344060087 18.888089013501055 48-49 23.426141252822717 28.822685457267326 28.318175005851714 19.432998284058243 50-51 19.844554873931326 29.253439040285055 31.40931188883257 19.492694196951046 52-53 22.042947378347474 31.079847703854384 26.62513492623471 20.252069991563427 54-55 20.256785042511236 29.27852984711447 27.814001343789517 22.65068376658477 56-57 23.292098048593196 26.958816357598497 28.261718881561666 21.487366712246644 58-59 25.609168768797087 27.013582737495835 26.91759761013872 20.459650883568354 60-61 21.78322034569082 28.46446551975298 30.71397355828133 19.038340576274866 62-63 20.062263795043147 28.6053044644 29.95365161916182 21.37878012139503 64-65 19.920224752229203 31.860229048467463 27.826229798203162 20.393316401100172 66-67 21.96747599103564 27.032708450363785 27.169734092771495 23.83008146582907 68-69 20.708263097537525 29.09614319177277 27.315255299048722 22.88033841164098 70-71 23.709316864320083 28.32030378425256 26.891221157124995 21.079158194302362 72-73 22.324587475140735 26.60475917035311 30.58417797296592 20.486475381540238 74-75 20.17696596575189 31.419920753465142 27.98660588741469 20.41650739336828 76-77 21.660523067544787 28.22690509107626 26.79866564058177 23.31390620079718 78-79 21.886508723686227 29.332668735675927 27.02002044311375 21.760802097524092 80-81 23.242843647226625 27.56477722226245 27.53311902304144 21.659260107469482 82-83 21.958750039993735 26.45438272405333 29.672657587948333 21.914209648004608 84-85 20.07192148753372 28.264757292343756 29.05242471903301 22.610896501089513 86-87 20.513712153807163 29.095564504925193 26.846705605686434 23.54401773558121 88-89 20.233125590433833 29.358938305242294 25.52821200216219 24.87972410216168 90-91 21.91429384534296 25.90086942171584 26.8950715929968 25.289765139944397 92-93 22.524892943084282 28.456763824781973 26.762881771781434 22.255461460352315 94-95 21.291942974730652 28.62588028465484 27.330923040135254 22.75125370047925 96-97 18.644478797247736 29.015489072777424 30.180116462738997 22.159915667235847 98-99 20.679531048976486 30.290433722441882 25.99892899723156 23.03110623135007 100-101 18.53949660634913 28.067905013139296 26.863687304184115 26.528911076327468 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 134.0 1 94.0 2 152.5 3 221.0 4 151.0 5 115.5 6 165.5 7 259.5 8 360.0 9 431.0 10 448.0 11 466.0 12 479.5 13 520.0 14 599.0 15 649.0 16 726.0 17 804.5 18 857.0 19 950.5 20 1074.0 21 1189.5 22 1333.5 23 1593.5 24 1952.0 25 2367.5 26 3008.5 27 3943.0 28 5114.5 29 6554.0 30 8102.0 31 9576.0 32 11096.0 33 12233.5 34 13122.5 35 14261.5 36 15185.0 37 16049.5 38 16915.0 39 17374.0 40 17794.5 41 18198.0 42 18482.0 43 19123.0 44 19478.0 45 19822.0 46 21554.5 47 31986.0 48 55375.5 49 54922.0 50 33337.5 51 22695.5 52 16620.0 53 13196.5 54 11479.0 55 10237.5 56 8791.5 57 7154.5 58 5796.0 59 4683.0 60 3647.0 61 2775.0 62 2077.0 63 1474.5 64 998.5 65 688.0 66 417.5 67 219.0 68 108.5 69 57.5 70 35.5 71 24.0 72 10.5 73 6.5 74 5.5 75 3.5 76 3.5 77 1.5 78 0.5 79 0.5 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08234499690995768 2 0.0026943148273196786 3 0.01886020379123775 4 0.0 5 0.0 6 0.002020736120489759 7 0.0 8 0.0 9 0.0 10-11 5.893813684761797E-4 12-13 0.0 14-15 0.004209866917686998 16-17 0.0 18-19 0.0 20-21 0.013892560828367094 22-23 0.0 24-25 0.0 26-27 0.008419733835373996 28-29 0.011114048662693676 30-31 0.010356272617510015 32-33 0.005220234977931878 34-35 0.007240971098421637 36-37 0.02045995321995881 38-39 0.007914549805251556 40-41 0.008924917865496436 42-43 0.0 44-45 0.0 46-47 8.419733835373996E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.002525920150612199 58-59 1.6839467670747992E-4 60-61 0.007830352466897818 62-63 0.013303179459890914 64-65 0.05060260035059772 66-67 0.02669055625813557 68-69 0.0016839467670747993 70-71 8.419733835373996E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.6839467670747992E-4 86-87 0.0014313547520135794 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.6839467670747992E-4 96-97 8.419733835373996E-4 98-99 0.0011787627369523595 100-101 0.002273328135550979 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 593843.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.69254135502855 #Duplication Level Percentage of deduplicated Percentage of total 1 88.06527598760024 56.09101232782232 2 7.833543965456544 9.978766459525506 3 1.6969198294001546 3.24243409230712 4 0.7217237065629255 1.838736681086545 5 0.40704264466700535 1.2962790239356716 6 0.28070141042562397 1.0727151715169339 7 0.1803693854696919 0.8041729180246605 8 0.1346470715623384 0.6860811339054183 9 0.09929075543393785 0.5691672491983302 >10 0.5216640261495797 5.780506585175694 >50 0.031125131622577757 1.3554504228287678 >100 0.018991601588166773 2.507616446380415 >500 0.00342903917564039 1.7079209965094604 >1k 0.0047479003970405395 8.106156608457749 >5k 2.6377224428002997E-4 1.1380539868537365 >10k+ 2.6377224428002997E-4 3.824929896471659 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22767 3.833841604599195 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6774 1.140705540016469 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4891 0.8236183637762844 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4827 0.8128411044670056 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4376 0.7368951052719321 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4223 0.7111307197356878 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3872 0.6520241882113623 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3471 0.5844979228516628 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3055 0.5144457373413511 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2850 0.4799248286163178 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2472 0.41627164082089035 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2003 0.33729453744508225 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1840 0.3098462051417631 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1691 0.28475539831234853 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1678 0.28256626751515135 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1675 0.28206108348502884 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1502 0.25292880441463483 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1389 0.2339002059466896 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1281 0.21571358086228176 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1154 0.19432745692043182 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1000 0.16839467670747993 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 963 0.16216407366930316 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 954 0.16064852157893586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 931 0.1567754440146638 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 913 0.15374433983392918 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 870 0.14650336873550754 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 819 0.13791524022342605 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 819 0.13791524022342605 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 710 0.11956022046231075 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.051840301224398E-4 0.0 14-15 0.0 0.0 0.0 7.577760451836597E-4 0.0 16-17 0.0 0.0 0.0 8.419733835373996E-4 0.0 18-19 0.0 0.0 0.0 9.261707218911396E-4 0.0 20-21 0.0 0.0 0.0 0.0015997494287210592 0.0 22-23 0.0 0.0 0.0 0.0026943148273196786 0.0 24-25 0.0 0.0 0.0 0.004967642962870658 0.0 26-27 0.0 8.419733835373996E-5 0.0 0.007156773760067897 0.0 28-29 0.0 1.6839467670747992E-4 0.0 0.013892560828367094 0.0 30-31 0.0 1.6839467670747992E-4 0.0 0.027785121656734184 0.0 32-33 0.0 1.6839467670747992E-4 0.0 0.05136037639578138 0.0 34-35 0.0 1.6839467670747992E-4 0.0 0.07308328969104629 0.0 36-37 0.0 1.6839467670747992E-4 0.0 0.09253287485076023 0.0 38-39 0.0 1.6839467670747992E-4 0.0 0.13673647748647372 0.0 40-41 0.0 1.6839467670747992E-4 0.0 0.2208496185018599 0.0 42-43 0.0 1.6839467670747992E-4 0.0 0.3484927834461297 0.0 44-45 0.0 1.6839467670747992E-4 0.0 0.4519713122828761 0.0 46-47 0.0 1.6839467670747992E-4 0.0 0.7259494512859459 0.0 48-49 0.0 1.6839467670747992E-4 0.0 0.9238973937555885 0.0 50-51 0.0 1.6839467670747992E-4 0.0 1.0496040199177226 0.0 52-53 0.0 1.6839467670747992E-4 0.0 1.2337435988973517 0.0 54-55 0.0 1.6839467670747992E-4 0.0 1.3879931227614033 0.0 56-57 0.0 1.6839467670747992E-4 0.0 1.8445952886537351 0.0 58-59 0.0 1.6839467670747992E-4 0.0 2.4064441274882418 0.0 60-61 0.0 1.6839467670747992E-4 0.0 2.883253654585471 0.0 62-63 0.0 1.6839467670747992E-4 0.0 3.294894441796906 0.0 64-65 0.0 1.6839467670747992E-4 0.0 3.899936515206881 0.0 66-67 0.0 1.6839467670747992E-4 0.0 4.794617432553721 0.0 68-69 0.0 1.6839467670747992E-4 0.0 5.636927605444537 0.0 70-71 0.0 1.6839467670747992E-4 0.0 6.059429849303604 0.0 72-73 0.0 1.6839467670747992E-4 0.0 6.34730054913504 0.0 74-75 0.0 1.6839467670747992E-4 0.0 6.744711986164694 0.0 76-77 0.0 1.6839467670747992E-4 0.0 7.127641480997503 0.0 78-79 0.0 1.6839467670747992E-4 0.0 7.551154092916814 0.0 80-81 0.0 1.6839467670747992E-4 0.0 8.374519864678039 0.0 82-83 0.0 1.6839467670747992E-4 0.0 9.264317336400362 0.0 84-85 0.0 1.6839467670747992E-4 0.0 10.327224535777976 0.0 86-87 0.0 1.6839467670747992E-4 0.0 11.267961397204312 0.0 88-89 0.0 1.6839467670747992E-4 0.0 12.451018198412712 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 115 0.0 66.10207 2 CTACCGC 25 0.0050239083 56.989037 4 ATAACGC 145 0.0 55.702396 3 TACCTGG 840 0.0 54.298138 2 TAACGCA 140 0.0 54.275272 4 ACCTGGG 830 0.0 51.517807 3 GTACCTG 1100 0.0 41.9242 1 GTACCCG 50 0.0016080375 38.034325 1 CGGTCGT 50 0.0016167143 37.99269 6 GAGTACG 435 0.0 36.06703 1 AGTACGC 400 0.0 35.63315 2 TCACGCA 95 8.7035914E-7 34.993267 4 TACGCAG 425 0.0 34.640396 4 GTATAAC 235 0.0 34.392742 1 GTACGCA 440 0.0 33.473568 3 TATCACG 100 1.2954733E-6 33.257607 2 CCGGTCG 60 0.003953148 31.660576 5 TACACGG 110 2.7256483E-6 30.234188 2 CCTGGGG 550 0.0 30.22146 4 ACACGGG 100 4.9414186E-5 28.50652 3 >>END_MODULE