##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003798_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1924178 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.689412310087736 31.0 31.0 34.0 30.0 34.0 2 31.88964014763707 31.0 31.0 34.0 30.0 34.0 3 31.955182420753175 31.0 31.0 34.0 30.0 34.0 4 35.6016891368678 37.0 35.0 37.0 33.0 37.0 5 35.52739091705653 37.0 35.0 37.0 33.0 37.0 6 35.613143378627136 37.0 35.0 37.0 33.0 37.0 7 35.56886577021461 37.0 35.0 37.0 33.0 37.0 8 35.581190513559555 37.0 35.0 37.0 33.0 37.0 9 37.13547499243833 39.0 37.0 39.0 33.0 39.0 10-11 37.03194532938221 39.0 37.0 39.0 33.0 39.0 12-13 37.01560952261173 39.0 37.0 39.0 33.0 39.0 14-15 37.970250673274506 40.0 37.0 41.0 33.0 41.0 16-17 38.097539312891016 40.0 37.0 41.0 33.0 41.0 18-19 38.16955785795285 40.0 37.0 41.0 33.0 41.0 20-21 38.07344071078663 40.0 37.0 41.0 33.0 41.0 22-23 37.96644827037831 40.0 37.0 41.0 33.0 41.0 24-25 37.78196092045539 39.5 37.0 41.0 32.5 41.0 26-27 37.48500736418356 39.0 36.5 41.0 31.5 41.0 28-29 37.42486713807142 39.0 36.0 41.0 32.0 41.0 30-31 37.29574888601782 39.0 36.0 40.0 31.5 41.0 32-33 37.04107832019699 39.0 36.0 40.0 31.0 41.0 34-35 36.93097494098779 39.0 35.5 40.0 30.5 41.0 36-37 36.71352312519943 38.5 35.5 40.0 30.5 41.0 38-39 36.48304470792203 38.0 35.0 40.0 30.0 41.0 40-41 36.837720574707745 39.0 35.5 40.5 31.0 41.0 42-43 36.72505558217587 39.0 35.0 41.0 30.0 41.0 44-45 36.55800346953348 39.0 35.0 41.0 30.0 41.0 46-47 36.313498543274065 39.0 35.0 40.5 30.0 41.0 48-49 35.999646602341365 39.0 35.0 40.0 28.5 41.0 50-51 35.67135576854116 38.0 35.0 40.0 28.0 41.0 52-53 35.6666610365569 39.0 35.0 40.0 28.0 41.0 54-55 35.49941455520228 38.5 34.5 40.0 28.0 41.0 56-57 35.1058059597397 38.0 34.0 40.0 26.5 41.0 58-59 34.819949349800275 38.0 34.0 40.0 26.0 41.0 60-61 34.133491288228015 37.5 33.0 40.0 24.0 41.0 62-63 34.09118647027458 37.0 33.0 40.0 25.5 41.0 64-65 33.703427645467315 36.5 32.5 39.5 24.0 41.0 66-67 33.216345369295354 36.0 32.0 39.0 22.5 40.0 68-69 32.791703522231316 36.0 32.0 39.0 22.0 40.0 70-71 32.191765262881084 35.0 31.0 37.5 21.5 40.0 72-73 31.710291355581447 35.0 30.5 37.0 20.5 39.0 74-75 31.424805813183603 34.5 31.0 37.0 20.0 39.0 76-77 30.51778785538552 34.0 29.5 35.5 20.0 38.0 78-79 30.878723278199836 34.0 31.0 36.0 20.0 38.0 80-81 30.51556638730928 34.0 30.0 35.5 18.0 37.0 82-83 30.166760819425228 34.0 29.5 35.0 14.5 37.0 84-85 29.832483013525774 34.0 29.5 35.0 11.5 36.0 86-87 29.741173113921896 34.0 30.0 35.0 8.5 36.0 88-89 29.397205456044087 34.0 29.0 35.0 7.0 36.0 90-91 28.969066791118077 34.0 29.0 35.0 2.0 35.0 92-93 28.711940631272157 34.0 29.0 35.0 2.0 35.0 94-95 28.297327222325585 34.0 29.0 35.0 2.0 35.0 96-97 27.83113828346442 34.0 28.0 35.0 2.0 35.0 98-99 27.609702168926162 34.0 29.0 35.0 2.0 35.0 100-101 26.51285847774998 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09724085678630345 1101 2 0.09348584729750087 1101 3 0.13773266330544587 1101 4 0.13182408841559123 1101 5 0.04567637717327244 1101 6 0.16887027802818722 1101 7 0.12583363840269612 1101 8 0.11749693257065275 1101 9 0.19888777917369538 1101 10-11 0.2145964820699504 1101 12-13 0.253817976907861 1101 14-15 0.26196711787362403 1101 16-17 0.22410564109512165 1101 18-19 0.09181020557414143 1101 20-21 0.016143286057634043 1101 22-23 0.04430350355396939 1101 24-25 0.169516562749628 1101 26-27 0.3487368502133492 1101 28-29 0.24293374284514613 1101 30-31 0.22641857309344715 1101 32-33 0.43961525153341796 1101 34-35 0.35096530708738527 1101 36-37 0.3247662198206953 1101 38-39 0.25765964878478087 1101 40-41 0.18532255547714982 1101 42-43 0.10965728782542072 1101 44-45 0.25970080873011625 1101 46-47 0.23912902550320325 1101 48-49 0.3350806496918537 1101 50-51 0.3270657751700554 1101 52-53 0.5126547179868766 1101 54-55 0.48401815247503777 1101 56-57 0.34774861545877656 1101 58-59 0.3465659288512555 1101 60-61 0.4437757616564397 1101 62-63 0.06403616727045147 1101 64-65 -0.1101605395621661 1101 66-67 -0.09176657537141608 1101 68-69 -0.0803606467941691 1101 70-71 0.05660215175149119 1101 72-73 0.04230804478576644 1101 74-75 0.004538870568097764 1101 76-77 0.03610051574836248 1101 78-79 0.10418379436345049 1101 80-81 0.30671635813707354 1101 82-83 0.20881278987337382 1101 84-85 0.06763036649106269 1101 86-87 0.04652277887740652 1101 88-89 -0.010537109633940389 1101 90-91 0.0036421224447913403 1101 92-93 0.06126766995440036 1101 94-95 -0.03486185827006594 1101 96-97 0.15657956511038762 1101 98-99 0.009298837810788996 1101 100-101 0.005276388341258098 1102 1 -0.07028154329423586 1102 2 0.010211240767080909 1102 3 -0.022908913033806044 1102 4 -0.05800470897134602 1102 5 -0.2021339520324048 1102 6 -0.038606622762664244 1102 7 -0.08631553886275611 1102 8 -0.09085317984922625 1102 9 -0.020537928624108304 1102 10-11 -0.04014116292932357 1102 12-13 0.04606981421783729 1102 14-15 -0.031584749079407004 1102 16-17 -0.022667181259102165 1102 18-19 -0.14974187391398885 1102 20-21 -0.11862144318384793 1102 22-23 -0.07970303469675599 1102 24-25 -0.10879255276879007 1102 26-27 -0.14705684252801632 1102 28-29 0.016035049376682764 1102 30-31 -0.024906974743728938 1102 32-33 -0.03795215844677813 1102 34-35 -0.10317500243083799 1102 36-37 -0.2107624472486762 1102 38-39 -0.27333866491701997 1102 40-41 -0.09124433155485434 1102 42-43 0.08057982996889024 1102 44-45 0.13455259346562087 1102 46-47 0.04397811160228571 1102 48-49 -0.012823222921319655 1102 50-51 -0.07815161187873088 1102 52-53 0.05650651111193383 1102 54-55 0.0638163420116058 1102 56-57 -0.04840247182603008 1102 58-59 -0.12112628956770521 1102 60-61 -0.002895407398447958 1102 62-63 -0.11328836543199117 1102 64-65 -0.1934882798666564 1102 66-67 -0.11374298397572602 1102 68-69 -0.19555711591505798 1102 70-71 -0.005469981541580182 1102 72-73 -0.03993639574355612 1102 74-75 -0.005770479857531541 1102 76-77 0.1325495017322318 1102 78-79 0.11451411864726424 1102 80-81 0.060335047802276165 1102 82-83 -0.10958540861203403 1102 84-85 -0.0706303553452372 1102 86-87 -0.15350875182273072 1102 88-89 -0.028362442139506783 1102 90-91 0.07324212300409627 1102 92-93 0.1125204385036831 1102 94-95 0.05788629043738425 1102 96-97 -0.044253866100952166 1102 98-99 -0.2756327734289634 1102 100-101 -0.20151501685130668 1103 1 0.0019085130620766222 1103 2 -0.017597774671820332 1103 3 -0.04000525521673737 1103 4 -0.005463557964091592 1103 5 0.070169202507266 1103 6 0.07857875567951567 1103 7 0.053014901803656755 1103 8 0.018597872699302798 1103 9 -0.04752488782283848 1103 10-11 0.05001727210787976 1103 12-13 -0.018932724065251705 1103 14-15 0.02520829385910872 1103 16-17 0.012123124189294288 1103 18-19 0.008997447863436037 1103 20-21 0.12373235087702739 1103 22-23 0.16356170906279743 1103 24-25 0.044331011042558544 1103 26-27 0.034386487356677264 1103 28-29 -0.024786445180588146 1103 30-31 0.18291700621236373 1103 32-33 0.2363691275024138 1103 34-35 0.12560760462985598 1103 36-37 0.23677863116821385 1103 38-39 0.4142704437707607 1103 40-41 0.39565572387562753 1103 42-43 0.43716635924220526 1103 44-45 0.3723306949250613 1103 46-47 0.4827017205386568 1103 48-49 0.4023431435067977 1103 50-51 0.46838917117862877 1103 52-53 0.3777637811569292 1103 54-55 0.17874951483691603 1103 56-57 0.29486122472811616 1103 58-59 0.3855987921202697 1103 60-61 0.39203776066683105 1103 62-63 0.17580529971314007 1103 64-65 0.18313485875549418 1103 66-67 0.153429697100961 1103 68-69 0.16198534528896147 1103 70-71 0.21688790138038883 1103 72-73 0.37222413418045974 1103 74-75 0.31488440908038484 1103 76-77 0.3582595129444677 1103 78-79 0.49252290041655655 1103 80-81 0.43375742691490515 1103 82-83 0.37077365908003657 1103 84-85 0.4034738020840045 1103 86-87 0.3762017744241639 1103 88-89 0.43287477392205176 1103 90-91 0.23481377839069495 1103 92-93 0.1758200780032233 1103 94-95 0.04294100334959339 1103 96-97 0.26292646433519096 1103 98-99 0.20704026119855357 1103 100-101 0.3276260914585265 1104 1 0.013086500820577385 1104 2 -0.12841291365410612 1104 3 -0.03920513564883876 1104 4 -0.05810256689591853 1104 5 -0.00490418323729358 1104 6 -0.03548944492074213 1104 7 -0.0018655314831192982 1104 8 -0.1366596684513155 1104 9 -0.17544287864633645 1104 10-11 -0.13588984627973133 1104 12-13 -0.12132836921672663 1104 14-15 -0.08324935842711767 1104 16-17 -0.22909421344007797 1104 18-19 -0.33246518397094604 1104 20-21 -0.35562619861847367 1104 22-23 -0.2509809970230563 1104 24-25 -0.15943241695580923 1104 26-27 -0.04168527138335065 1104 28-29 -0.1411594306995525 1104 30-31 -0.24087503944324595 1104 32-33 -0.24186124866510994 1104 34-35 -0.053660274122428575 1104 36-37 -0.10172500312267374 1104 38-39 0.07236724876399592 1104 40-41 -0.033588054176220794 1104 42-43 -0.040775163813101756 1104 44-45 0.25452836614604024 1104 46-47 0.14999646606487005 1104 48-49 0.20105148808120532 1104 50-51 0.17121213892111342 1104 52-53 0.14841973945407005 1104 54-55 0.1253141761279224 1104 56-57 0.02797989799817202 1104 58-59 -0.01838709659394766 1104 60-61 0.1161633789652683 1104 62-63 0.10826968929983849 1104 64-65 0.009962029375898851 1104 66-67 -0.018676543050027306 1104 68-69 0.011315227386198501 1104 70-71 0.1312407626368497 1104 72-73 0.21153878368758328 1104 74-75 0.020389245919236743 1104 76-77 -0.08467701587724363 1104 78-79 0.0794325131970588 1104 80-81 0.04660247255701222 1104 82-83 0.06707842362862948 1104 84-85 0.04972383968583571 1104 86-87 0.16191646618745992 1104 88-89 0.03600919140398062 1104 90-91 -0.10661924570360881 1104 92-93 -0.1884096032114222 1104 94-95 -0.055506982601652766 1104 96-97 0.046293380741172285 1104 98-99 -0.19069543358496688 1104 100-101 -0.20600439304963558 1105 1 0.07346881115480741 1105 2 0.08393979266157103 1105 3 0.0755394403272156 1105 4 0.10332132953809747 1105 5 -0.05159944287343876 1105 6 0.13964606422364767 1105 7 0.09229973636823985 1105 8 0.15578270488709478 1105 9 0.004970850159779161 1105 10-11 0.20937809914452288 1105 12-13 -0.017351753297617734 1105 14-15 0.20750150228850117 1105 16-17 0.03851400574086483 1105 18-19 -0.012016890664988011 1105 20-21 0.046685583203966985 1105 22-23 0.0169136047692362 1105 24-25 0.10888261332340932 1105 26-27 0.2386451556194018 1105 28-29 0.22690728808735372 1105 30-31 0.13900404751882434 1105 32-33 0.19884500848139197 1105 34-35 0.10524431761512432 1105 36-37 0.037465307529295444 1105 38-39 0.2793974454064312 1105 40-41 0.2542437158982622 1105 42-43 0.3948054717054461 1105 44-45 0.4486923797669746 1105 46-47 0.10371235883653185 1105 48-49 0.049555870571310834 1105 50-51 0.15798614854941917 1105 52-53 0.22601178951458678 1105 54-55 0.1901352862890633 1105 56-57 -0.0030424461166589367 1105 58-59 0.04518500911259338 1105 60-61 0.08638899411302958 1105 62-63 -0.062234312740379494 1105 64-65 0.09669180125229104 1105 66-67 0.06512342496710488 1105 68-69 0.18688762679169457 1105 70-71 0.30602456466913 1105 72-73 0.3812744389498093 1105 74-75 0.2523914314157345 1105 76-77 0.1107531172175058 1105 78-79 0.04792088857149679 1105 80-81 0.31048654194940184 1105 82-83 0.415194386637193 1105 84-85 0.3245815106635739 1105 86-87 0.38472540404243105 1105 88-89 0.42438490553142216 1105 90-91 0.39037687245325614 1105 92-93 0.42006878535334025 1105 94-95 0.46140709843417227 1105 96-97 0.4588935101359439 1105 98-99 0.5063146378852643 1105 100-101 0.6174774394228066 1106 1 -0.010970288112147841 1106 2 -0.009835708342862404 1106 3 -0.0245958522538281 1106 4 0.02451730208427705 1106 5 0.11589317700584445 1106 6 0.00918379241790035 1106 7 0.09690031984278136 1106 8 0.08389819077297034 1106 9 0.053751309773126366 1106 10-11 0.012988001105078695 1106 12-13 -0.10205019705728091 1106 14-15 -0.03681502455848573 1106 16-17 -0.027539952939704904 1106 18-19 -4.719430135651237E-4 1106 20-21 0.059227020530158825 1106 22-23 -0.030632837399977575 1106 24-25 0.08334447711308002 1106 26-27 -0.022283433477213066 1106 28-29 -0.003972304405152727 1106 30-31 -0.073086722764657 1106 32-33 -0.016853323160539446 1106 34-35 -0.12265042926465242 1106 36-37 -0.0923427515393982 1106 38-39 0.09330325148667384 1106 40-41 -0.2733170183919711 1106 42-43 -0.10089128797655889 1106 44-45 0.11922258104720385 1106 46-47 -0.08914761998823906 1106 48-49 -0.07735533218609447 1106 50-51 -0.0196532704264456 1106 52-53 -0.014498224794870396 1106 54-55 -0.0308954281898437 1106 56-57 -0.05035728426258146 1106 58-59 -0.1059443458521443 1106 60-61 -0.10192255909897341 1106 62-63 -0.12486102311369507 1106 64-65 0.05524320549796613 1106 66-67 -0.089011436480817 1106 68-69 -0.12645715719095563 1106 70-71 -0.015626756123971575 1106 72-73 -0.030567071657451095 1106 74-75 0.008890358784249486 1106 76-77 -0.14196732704611037 1106 78-79 0.05121290209035578 1106 80-81 0.011134224117597569 1106 82-83 -0.033070938238378034 1106 84-85 -0.06585447126813548 1106 86-87 0.12598181035708578 1106 88-89 0.2823974800550353 1106 90-91 0.23755000303458118 1106 92-93 0.2672130196123561 1106 94-95 0.29012154728883743 1106 96-97 0.09713799498386777 1106 98-99 0.058275137768522 1106 100-101 0.0942434959532612 1107 1 -0.19134920648443554 1107 2 -0.08798074473343931 1107 3 -0.009246710093702148 1107 4 -0.06641221847294787 1107 5 -0.15729039296564906 1107 6 -0.05460541447261846 1107 7 -0.03923853773761721 1107 8 0.08568557437004642 1107 9 -0.03368308236007067 1107 10-11 0.059928250597423016 1107 12-13 0.017289513555695635 1107 14-15 0.0893545069802002 1107 16-17 -0.02640111852695526 1107 18-19 -0.04733718726422609 1107 20-21 0.1491486038531633 1107 22-23 -0.0984002180547705 1107 24-25 -3.157613051314456E-4 1107 26-27 0.11867487287546652 1107 28-29 -0.022876521263548 1107 30-31 0.1253380513218616 1107 32-33 0.18009972645412375 1107 34-35 0.11118478277140298 1107 36-37 -0.0023699098079887904 1107 38-39 0.19829191092581055 1107 40-41 0.02913669555483267 1107 42-43 0.12733344510324685 1107 44-45 -0.1374100253241295 1107 46-47 -0.08351411818753718 1107 48-49 0.08059296458677068 1107 50-51 0.09550048163112734 1107 52-53 0.16013395992537482 1107 54-55 0.10852570510171233 1107 56-57 -0.02686283349208196 1107 58-59 0.0788372933280499 1107 60-61 0.3207826569798371 1107 62-63 0.3079354387239803 1107 64-65 0.270793355913888 1107 66-67 0.22632580330311924 1107 68-69 0.2776793384483156 1107 70-71 0.4331637771140606 1107 72-73 0.3956355896096113 1107 74-75 0.29433540339933373 1107 76-77 0.36842669083699064 1107 78-79 0.2655072215003216 1107 80-81 0.21960883180253532 1107 82-83 0.40528126900081674 1107 84-85 0.26616405010343414 1107 86-87 0.348337890067679 1107 88-89 0.26673832588248203 1107 90-91 0.23417375357467662 1107 92-93 0.21880365200790308 1107 94-95 0.4280731280749883 1107 96-97 0.5489106381685822 1107 98-99 0.3347906490841801 1107 100-101 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-0.74765868714492 2316 72-73 -0.5117432699894557 2316 74-75 -0.5924421640901905 2316 76-77 -0.5927554552800451 2316 78-79 -0.5789175625892966 2316 80-81 -0.5957044862860421 2316 82-83 -0.5726471887469842 2316 84-85 -0.7289517087885393 2316 86-87 -0.5789730797260795 2316 88-89 -0.4784390141672965 2316 90-91 -0.41108610522656974 2316 92-93 -0.55736252406221 2316 94-95 -0.7353155393498163 2316 96-97 -0.6701785286109789 2316 98-99 -0.9409466826453077 2316 100-101 -1.3358747853574187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 12.0 5 21.0 6 87.0 7 226.0 8 567.0 9 983.0 10 1741.0 11 3154.0 12 4863.0 13 6854.0 14 7833.0 15 9886.0 16 12082.0 17 15171.0 18 14490.0 19 11530.0 20 10813.0 21 11559.0 22 12147.0 23 13264.0 24 15255.0 25 18769.0 26 23148.0 27 29039.0 28 36021.0 29 45026.0 30 56651.0 31 69882.0 32 86775.0 33 109416.0 34 142778.0 35 191651.0 36 272379.0 37 347836.0 38 289722.0 39 52507.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.003153573573414 18.944007820238507 13.30215290542139 18.750685700766685 2 14.22953167820723 26.888393068282397 35.50091134322168 23.381163910288695 3 28.978026325982782 24.55984281567497 28.438758060817936 18.023372797524313 4 12.090045723420598 16.281549835826002 42.64548290230945 28.98292153844395 5 11.29022366953577 41.797432462069516 33.594137340724195 13.318206527670517 6 26.564831382951354 40.136153413995665 16.438349757009966 16.860665446043015 7 27.259900071614997 34.16321151161691 21.49312589583708 17.083762520931014 8 23.66329934132913 28.2591319514099 19.11850151077499 28.959067196485982 9 21.382117454830063 24.442593148866685 24.926747941198787 29.248541455104466 10-11 26.044359928883587 24.401053129948565 27.612647329834335 21.941939611333517 12-13 32.2969080823084 22.877405312814094 24.958397819744327 19.867288785133184 14-15 22.96252139609469 26.960261667059488 30.709681208258615 19.36753572858721 16-17 19.465350918678002 29.062020249685837 29.957701418475835 21.51492741316032 18-19 18.757204375063015 34.601190742228624 27.523415193396865 19.11818968931149 20-21 22.948132251155883 26.796210213255417 25.66284494925961 24.592812586329085 22-23 19.997422275901712 28.334696686065424 27.273464305277372 24.394416732755495 24-25 25.404198572065578 27.84056360690123 26.77966903269864 19.975568788334552 26-27 24.110250392598694 23.936579231652587 32.812856915285224 19.1403134604635 28-29 19.27088991025482 33.603325982339946 28.335220891027717 18.79056321637752 30-31 22.029197626601356 27.26328903654485 26.583504505216236 24.124008831637553 32-33 22.66118138788883 28.383641042192576 28.306824270981295 20.648353298937298 34-35 25.97631115555491 25.562283916866075 28.98320606560819 19.478198861970824 36-37 24.10370194022046 24.764606752669973 31.92498500365422 19.206706303455345 38-39 20.733188304143415 29.086287516947284 31.680492603745204 18.500031575164094 40-41 20.966312540575323 30.399591245034152 27.62458995857873 21.00950625581179 42-43 19.289275732286722 31.030731044633086 25.113710893690712 24.566282329389484 44-45 23.129902742885534 25.169682846389474 26.303777509149363 25.396636901575636 46-47 26.020007514901177 29.642101532239494 26.2757532181291 18.062137734730225 48-49 24.51051825766639 28.36117552534121 27.82065900348097 19.30764721351143 50-51 18.137848993180462 29.449770239551643 32.640145558259164 19.77223520900873 52-53 22.229102505069697 32.61036660849464 25.06550849245756 20.095022393978105 54-55 19.739779791682473 28.184398740657052 27.239060003804223 24.836761463856256 56-57 24.069713809066588 25.702718469559976 27.78311490383428 22.44445281753915 58-59 29.148333037535334 25.60603292790385 25.12374793645168 20.121886098109137 60-61 22.65940529784355 28.267700431113457 31.564890644170145 17.50800362687285 62-63 20.303973154348462 28.26987759676114 30.190780435649287 21.23536881324111 64-65 19.4821295250692 34.28472347868042 26.506580028241718 19.72656696800867 66-67 22.74655210548791 27.199113812552746 24.96989598161712 25.084438100342222 68-69 20.420988515376756 29.223891425666977 26.292240993109644 24.06287906584663 70-71 25.420083396932135 28.35862084019018 25.482707752401488 20.738588010476196 72-73 24.10236994706311 24.725233320410066 31.15839594881555 20.01400078371128 74-75 19.690361206281644 33.772352082902955 27.116183408235468 19.421103302579933 76-77 22.22762135311806 27.538590504620675 25.57567439187019 24.658113750391074 78-79 22.69511968227472 28.853437675724386 27.150606648657245 21.30083599334365 80-81 25.660964230171075 26.830139111386554 27.343319418296907 20.165577240145467 82-83 23.393573775399158 25.79613216656671 30.47223281837751 20.33806123965662 84-85 20.313328681995294 29.384555219048135 29.951576712647356 20.350539386309215 86-87 21.203782309085717 30.36101048322951 26.18130896540506 22.253898242279718 88-89 19.925859250027806 30.590387167923133 24.391688294949844 25.092065287099217 90-91 23.067642390672795 24.591150091103838 25.97189553149449 26.369311986728878 92-93 25.074837151240686 28.956546639656 26.298164722806312 19.670451486297004 94-95 23.443347467514684 27.94711713111632 27.725411955345063 20.884123446023935 96-97 17.555883026262418 28.99137838518368 32.15325213318639 21.299486455367507 98-99 21.403207981417438 32.03782203179086 25.086251497074453 21.472718489717252 100-101 18.89719236145199 28.87488914002281 26.453106976727263 25.774811521797936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 255.0 1 208.5 2 376.0 3 534.0 4 383.5 5 316.0 6 444.0 7 715.0 8 1010.5 9 1195.0 10 1217.0 11 1185.5 12 1277.0 13 1450.5 14 1653.5 15 1819.0 16 1928.0 17 2090.5 18 2357.0 19 2620.0 20 2787.0 21 3068.0 22 3548.0 23 4236.5 24 5048.5 25 6197.0 26 7807.0 27 9975.5 28 13248.0 29 16985.0 30 20585.0 31 24289.0 32 27731.0 33 31008.0 34 34617.5 35 38070.5 36 41221.5 37 43988.5 38 46678.0 39 48975.0 40 50344.5 41 51152.5 42 52285.5 43 54523.5 44 55809.5 45 58141.0 46 67516.5 47 131710.5 48 272079.5 49 265939.5 50 129089.0 51 66231.5 52 44239.5 53 34377.0 54 28844.0 55 24323.5 56 19899.5 57 15731.0 58 12456.5 59 10097.5 60 8064.5 61 6070.5 62 4371.0 63 3005.5 64 1966.5 65 1256.0 66 749.0 67 425.0 68 220.0 69 115.5 70 66.5 71 35.5 72 24.5 73 14.5 74 11.5 75 8.0 76 3.0 77 2.5 78 3.0 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050047344892208516 2 0.004937173172128566 3 0.04318727269514567 4 0.0 5 0.0 6 0.0012992560979285701 7 0.0 8 0.0 9 0.0 10-11 4.677321952542852E-4 12-13 0.0 14-15 0.0027804080495671397 16-17 0.0 18-19 0.0 20-21 0.011303528051978559 22-23 0.0 24-25 0.0 26-27 0.007249849026441421 28-29 0.009094792685499991 30-31 0.009666465368588562 32-33 0.006184459026139994 34-35 0.00779553658757142 36-37 0.019540811712845694 38-39 0.010264123173635703 40-41 0.014915460004219986 42-43 0.0 44-45 0.0 46-47 5.1970243917142796E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0013252412198871415 58-59 2.338660976271426E-4 60-61 0.010731855368889988 62-63 0.015123340979888555 64-65 0.04064073074320567 66-67 0.022425160250247118 68-69 0.0020788097566857124 70-71 3.378065854614282E-4 72-73 0.0 74-75 2.5985121958571398E-5 76-77 0.0 78-79 0.0 80-81 2.5985121958571398E-5 82-83 0.0 84-85 2.5985121958571398E-5 86-87 4.937173172128566E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 5.1970243917142796E-5 96-97 1.2992560979285702E-4 98-99 4.417470732957138E-4 100-101 8.575090246328561E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1924178.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.97439604247123 #Duplication Level Percentage of deduplicated Percentage of total 1 82.49951320215109 37.92865293266778 2 11.28640200888104 10.377710317016797 3 2.5833782036548807 3.5630775798695216 4 1.077756985566773 1.9819690596794708 5 0.581011393570065 1.3355823956589143 6 0.3799134487903129 1.0479774813928175 7 0.24851115175279706 0.7997605078157632 8 0.19378220718609887 0.7127215951326338 9 0.14949323593434838 0.6185575111064692 >10 0.8812593529643102 7.651184635360093 >50 0.07226320811596539 2.283918371122984 >100 0.03685104700068008 3.2050443021041737 >500 0.0037417275430509027 1.1362423645630604 >1k 0.0037417275430509027 4.26733369031799 >5k 9.070854649820369E-4 3.0229249310002233 >10k+ 0.00147401388059581 20.067342325191344 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 112400 5.841455416286851 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 37414 1.9444147059159806 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 34161 1.7753555024535153 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 33515 1.7417827248830409 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 27665 1.4377567979677555 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 22685 1.1789449832603844 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 21457 1.115125523730133 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 19524 1.014667042238296 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 17809 0.9255380739203961 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 17148 0.8911857426911647 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 16440 0.8543908099978277 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 13845 0.7195280270328421 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 10897 0.5663197479651051 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 9069 0.47131814208456807 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8283 0.4304695303656938 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 8193 0.42579220841315096 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 8034 0.4175289396303253 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7500 0.389776829378571 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6540 0.33988539521811395 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5190 0.2697255659299711 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5181 0.26925783373471684 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4991 0.25938348739045974 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 4841 0.25158795080288826 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4594 0.238751300555354 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4562 0.23708825275000545 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4229 0.2197821615255969 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3979 0.20678960054631124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3904 0.2028918322525255 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3777 0.19629161127504838 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 3099 0.16105578589922553 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2859 0.14858292735911127 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2710 0.140839361015457 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2459 0.12779482979225415 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2458 0.127742859548337 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2409 0.125196317596397 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2365 0.12290962686404272 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2333 0.12124657905869417 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2217 0.11521803076430558 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2134 0.11090450051918274 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 2098 0.10903357173816561 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 2010 0.10446019027345703 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1979 0.1028491127120256 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.59851219585714E-5 0.0 12-13 0.0 0.0 0.0 7.79553658757142E-5 0.0 14-15 0.0 0.0 0.0 1.559107317514284E-4 0.0 16-17 0.0 0.0 0.0 3.637917074199996E-4 0.0 18-19 0.0 0.0 0.0 7.275834148399992E-4 0.0 20-21 0.0 0.0 0.0 0.0010394048783428562 0.0 22-23 0.0 0.0 0.0 0.0017929734151414267 0.0 24-25 0.0 0.0 0.0 0.0026764675617328543 0.0 26-27 0.0 0.0 0.0 0.0036639021961585674 0.0 28-29 0.0 0.0 0.0 0.006574235855518564 0.0 30-31 0.0 0.0 0.0 0.015409177321432842 0.0 32-33 0.0 0.0 0.0 0.028687574642262827 0.0 34-35 0.0 0.0 0.0 0.04105649269454281 0.0 36-37 0.0 0.0 0.0 0.053451395868781365 0.0 38-39 2.59851219585714E-5 0.0 0.0 0.07426547855759706 0.0 40-41 5.19702439171428E-5 0.0 0.0 0.09996476417462417 0.0 42-43 5.19702439171428E-5 0.0 0.0 0.12290962686404272 0.0 44-45 5.19702439171428E-5 0.0 0.0 0.13114691052490984 0.0 46-47 5.19702439171428E-5 0.0 0.0 0.14348984345523128 0.0 48-49 5.19702439171428E-5 0.0 0.0 0.16032820248438556 0.0 50-51 5.19702439171428E-5 0.0 0.0 0.1804406868803198 0.0 52-53 5.19702439171428E-5 0.0 0.0 0.2282273261621326 0.0 54-55 5.19702439171428E-5 0.0 0.0 0.25506995714533687 0.0 56-57 5.19702439171428E-5 2.59851219585714E-5 0.0 0.3344804898507311 0.0 58-59 5.19702439171428E-5 5.19702439171428E-5 0.0 0.48594776574724374 0.0 60-61 5.19702439171428E-5 5.19702439171428E-5 0.0 0.5928505574848065 0.0 62-63 5.19702439171428E-5 5.19702439171428E-5 0.0 0.6397277174980693 0.0 64-65 5.19702439171428E-5 5.19702439171428E-5 0.0 0.7453052680157449 0.0 66-67 5.19702439171428E-5 1.039404878342856E-4 0.0 1.073783194694046 0.0 68-69 5.19702439171428E-5 1.039404878342856E-4 0.0 1.4399135630903168 0.0 70-71 5.19702439171428E-5 1.039404878342856E-4 0.0 1.6550703729072882 0.0 72-73 5.19702439171428E-5 1.039404878342856E-4 0.0 1.8728516800420751 0.0 74-75 5.19702439171428E-5 1.039404878342856E-4 0.0 2.1429930079233834 0.0 76-77 5.19702439171428E-5 1.039404878342856E-4 0.0 2.241788441609872 0.0 78-79 5.19702439171428E-5 1.039404878342856E-4 0.0 2.42771198922345 0.0 80-81 5.19702439171428E-5 1.039404878342856E-4 0.0 2.692058634907997 0.0 82-83 5.19702439171428E-5 1.039404878342856E-4 0.0 2.889285710573554 0.0 84-85 5.19702439171428E-5 1.039404878342856E-4 0.0 3.3158834577674208 0.0 86-87 5.19702439171428E-5 1.039404878342856E-4 0.0 4.186722849965024 0.0 88-89 5.19702439171428E-5 1.039404878342856E-4 0.0 5.2743820997849475 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 290 0.0 58.98399 2 TACCTGG 2180 0.0 50.130383 2 ATAACGC 380 0.0 47.512413 3 ACCTGGG 2350 0.0 45.28843 3 GTACCTG 2720 0.0 40.8991 1 GTACGCA 1585 0.0 38.66941 3 AGTACGC 1575 0.0 38.011906 2 TACGCAG 1625 0.0 37.994125 4 GAGTACG 1670 0.0 36.723164 1 TAACGCA 485 0.0 36.231514 4 GTATAAC 590 0.0 35.454185 1 GTACCCG 195 0.0 34.131886 1 CCTGGGG 1605 0.0 32.84539 4 ACGCAGT 1845 0.0 32.691425 5 CGCAGTG 1860 0.0 32.427784 6 GTACACG 230 0.0 31.004896 1 GTAGGCA 1060 0.0 27.342049 3 TATCACG 285 0.0 26.67502 2 TCACGCA 290 0.0 26.202845 4 TACCCGG 240 2.2919266E-10 23.75744 2 >>END_MODULE