##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003797_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1247431 Sequences flagged as poor quality 0 Sequence length 121 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.916586969539797 31.0 31.0 34.0 28.0 34.0 2 30.85956658123776 31.0 31.0 34.0 27.0 34.0 3 30.96846719377665 31.0 31.0 34.0 27.0 34.0 4 34.48498794722914 37.0 35.0 37.0 32.0 37.0 5 34.46943758813113 37.0 35.0 37.0 32.0 37.0 6 34.57052133544861 37.0 35.0 37.0 32.0 37.0 7 34.570056379871914 37.0 35.0 37.0 32.0 37.0 8 34.55350636628399 37.0 35.0 37.0 31.0 37.0 9 36.03504161753235 39.0 35.0 39.0 32.0 39.0 10-11 35.89028892179207 39.0 35.0 39.0 30.0 39.0 12-13 35.77166753110994 39.0 35.0 39.0 30.0 39.0 14-15 36.76195236449952 39.0 36.0 41.0 31.0 41.0 16-17 36.809996705228585 39.0 36.0 41.0 31.0 41.0 18-19 36.75451668268626 39.0 36.0 41.0 30.5 41.0 20-21 36.66838807116386 39.0 36.0 41.0 30.0 41.0 22-23 36.57659702220002 39.0 36.0 41.0 30.0 41.0 24-25 36.33684588566422 39.0 36.0 41.0 29.0 41.0 26-27 36.18565716260058 39.0 36.0 41.0 29.0 41.0 28-29 36.04277150399501 39.0 35.0 40.0 29.0 41.0 30-31 35.78953625491109 38.5 35.0 40.0 27.5 41.0 32-33 35.53226992114193 38.0 35.0 40.0 27.0 41.0 34-35 35.503920858147666 38.0 35.0 40.0 27.0 41.0 36-37 35.35783221677191 38.0 35.0 40.0 26.5 41.0 38-39 35.35007948335419 38.0 35.0 40.0 26.5 41.0 40-41 35.51796892974441 39.0 35.0 40.0 27.0 41.0 42-43 35.38415671888866 39.0 35.0 40.0 26.0 41.0 44-45 35.15997838758216 38.0 35.0 40.0 25.0 41.0 46-47 34.883545061811034 38.0 34.0 40.0 24.0 41.0 48-49 34.590895608654904 38.0 34.0 40.0 23.0 41.0 50-51 33.75215422736809 37.5 33.0 39.5 22.5 40.5 52-53 33.91228693210286 38.0 33.0 39.5 23.0 40.5 54-55 34.27207116064937 38.0 34.0 40.0 23.0 41.0 56-57 34.01604377316261 38.0 33.5 40.0 21.0 41.0 58-59 33.70547188581973 38.0 33.0 40.0 20.0 41.0 60-61 33.364818975959395 38.0 32.0 40.0 18.5 41.0 62-63 33.155889985097375 37.0 32.0 40.0 18.0 41.0 64-65 32.868695743492026 37.0 32.0 39.5 17.5 41.0 66-67 32.43184833469747 36.0 31.0 39.0 13.0 41.0 68-69 32.006743058333484 36.0 31.0 39.0 11.5 40.5 70-71 31.47180044427307 35.0 30.5 38.5 8.0 40.0 72-73 31.061501998908156 35.0 30.0 37.5 7.5 40.0 74-75 30.714728910857595 35.0 30.0 37.0 6.5 39.0 76-77 30.30133089525593 34.5 29.5 36.5 2.0 39.0 78-79 29.732917892853393 34.0 29.0 36.0 2.0 39.0 80-81 29.225739539902406 34.0 29.0 36.0 2.0 37.5 82-83 28.833583981799393 34.0 28.5 35.0 2.0 37.0 84-85 28.570290861779128 34.0 28.0 35.0 2.0 37.0 86-87 28.426084088017696 34.0 28.0 35.0 2.0 36.0 88-89 28.100122972733562 34.0 27.0 35.0 2.0 36.0 90-91 27.722654800145257 34.0 26.0 35.0 2.0 35.5 92-93 27.439851983797098 34.0 26.0 35.0 2.0 35.0 94-95 27.178620300441466 33.0 25.0 35.0 2.0 35.0 96-97 26.87692184978568 33.0 25.0 35.0 2.0 35.0 98-99 26.658005933795135 33.0 25.0 35.0 2.0 35.0 100-101 26.378515124283425 33.0 24.5 35.0 2.0 35.0 102-103 25.96367454392267 32.5 23.5 35.0 2.0 35.0 104-105 25.55222252773901 32.0 20.0 35.0 2.0 35.0 106-107 25.121555420700624 32.0 19.0 35.0 2.0 35.0 108-109 24.82985832482919 32.0 18.0 34.0 2.0 35.0 110-111 24.482298820535966 32.0 14.0 34.0 2.0 35.0 112-113 23.87715953828308 31.0 4.5 34.0 2.0 35.0 114-115 23.284086654893137 31.0 2.0 34.0 2.0 35.0 116-117 22.551807274310164 31.0 2.0 34.0 2.0 35.0 118-119 21.720606590665135 30.0 2.0 34.0 2.0 35.0 120-121 20.060562467984198 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5383413843123357 1101 2 0.3848754142105726 1101 3 0.5485841234750808 1101 4 0.8723743171402774 1101 5 0.86648984939999 1101 6 0.8364256132807384 1101 7 0.5703588014121479 1101 8 0.7001502297023663 1101 9 0.7379166678994054 1101 10-11 0.8215222105924767 1101 12-13 0.9165776746521175 1101 14-15 0.9601865640311473 1101 16-17 0.753005277328775 1101 18-19 0.7661084359778911 1101 20-21 0.8652796503632132 1101 22-23 0.8719272130006814 1101 24-25 0.8338990467677476 1101 26-27 1.0313780552695633 1101 28-29 0.8676271496909465 1101 30-31 0.8889700374743938 1101 32-33 0.8291523965880714 1101 34-35 0.8299914578271554 1101 36-37 0.6530053040334778 1101 38-39 0.7618498353997794 1101 40-41 0.696519087793348 1101 42-43 0.7455329738051049 1101 44-45 0.8506635999287511 1101 46-47 0.7583443641090213 1101 48-49 0.7424886153759829 1101 50-51 0.419865228211755 1101 52-53 0.5371291702317436 1101 54-55 0.8252635412374971 1101 56-57 0.9118317837759022 1101 58-59 0.8573685167876164 1101 60-61 0.8094964446199171 1101 62-63 0.6197480406030635 1101 64-65 0.7790717562636544 1101 66-67 0.7674085198761951 1101 68-69 0.6493198318898692 1101 70-71 0.4109286807886221 1101 72-73 0.45903483055824523 1101 74-75 0.48492401435686006 1101 76-77 0.38946150679664626 1101 78-79 0.5345780298720655 1101 80-81 0.5359748079863351 1101 82-83 0.6787866317913576 1101 84-85 0.6536406835157855 1101 86-87 0.652965553887725 1101 88-89 0.7078855954019794 1101 90-91 0.6988474388325443 1101 92-93 0.7223335019121535 1101 94-95 0.7328572333025605 1101 96-97 0.6876417671641875 1101 98-99 0.5464994690441216 1101 100-101 0.6408617433919943 1101 102-103 0.4813871475527236 1101 104-105 0.4697003330523408 1101 106-107 0.539273533514411 1101 108-109 0.6871657430903397 1101 110-111 0.709430780676584 1101 112-113 0.7878123187566928 1101 114-115 0.67740396338802 1101 116-117 0.6885418217081529 1101 118-119 0.7239752499787038 1101 120-121 0.8667089555750103 1102 1 0.3523501881062501 1102 2 0.3904755962451354 1102 3 0.36345208020804165 1102 4 0.6484196926384058 1102 5 0.6894623275855807 1102 6 0.7403666397570561 1102 7 0.5797306348374249 1102 8 0.6007681431912033 1102 9 0.6148092462258319 1102 10-11 0.6372951563056901 1102 12-13 0.7423173674087735 1102 14-15 0.8077578540810677 1102 16-17 0.9003738793131575 1102 18-19 0.6531320071620925 1102 20-21 0.7743252566785443 1102 22-23 0.5967858715652881 1102 24-25 0.7511682663927743 1102 26-27 0.7219809574549032 1102 28-29 0.7380357016049004 1102 30-31 0.637013820715751 1102 32-33 0.400029103639568 1102 34-35 0.7161099036143028 1102 36-37 0.7177963216972714 1102 38-39 0.7194049791585186 1102 40-41 0.5964632387598101 1102 42-43 0.6791329201114564 1102 44-45 0.8259356013649466 1102 46-47 0.7255317609438237 1102 48-49 0.8525975632805896 1102 50-51 0.511451640881269 1102 52-53 0.5427316713517243 1102 54-55 0.5148480920906806 1102 56-57 0.6805324793378347 1102 58-59 0.8141841451315273 1102 60-61 0.8964403211157901 1102 62-63 0.8748509834406022 1102 64-65 0.7613067434595777 1102 66-67 0.7698449237331921 1102 68-69 0.5679922346096902 1102 70-71 0.20836325393230481 1102 72-73 0.27061036808132144 1102 74-75 0.3434827218678045 1102 76-77 0.39074136911390767 1102 78-79 0.6096556919502163 1102 80-81 0.5146595482405836 1102 82-83 0.6250493301481903 1102 84-85 0.6088485035809512 1102 86-87 0.589238041513358 1102 88-89 0.5095672324471039 1102 90-91 0.5414446999108193 1102 92-93 0.7853247728746418 1102 94-95 0.462752808978685 1102 96-97 0.4887945163171743 1102 98-99 0.7211083211841469 1102 100-101 0.4654386194366076 1102 102-103 0.7002669310756495 1102 104-105 0.6765753026996038 1102 106-107 0.5617014085937377 1102 108-109 0.618255620473473 1102 110-111 0.34982343730624876 1102 112-113 0.31463717978891026 1102 114-115 0.5880674728245161 1102 116-117 0.7215460904623221 1102 118-119 0.510529629257757 1102 120-121 0.6068634830572641 1103 1 0.5857244054609971 1103 2 0.6792140751742295 1103 3 0.7453233175461556 1103 4 0.7390415003838982 1103 5 0.8718786079966918 1103 6 0.9150253797879557 1103 7 0.7999316267092667 1103 8 0.6952849357528592 1103 9 0.9707973076283025 1103 10-11 1.0374298535207132 1103 12-13 0.9492339477904608 1103 14-15 1.0888473727151435 1103 16-17 0.9696227830886173 1103 18-19 0.8365385379501831 1103 20-21 0.908152093737769 1103 22-23 1.0551484491348972 1103 24-25 0.9609111140054054 1103 26-27 1.109033346911687 1103 28-29 1.0908077656573028 1103 30-31 1.0341664744709504 1103 32-33 0.9827234069781596 1103 34-35 0.9443770928551984 1103 36-37 0.9337343146508559 1103 38-39 0.8498452757471995 1103 40-41 0.9814198877988574 1103 42-43 0.8968284627107579 1103 44-45 0.9180540770047827 1103 46-47 0.9657190167110201 1103 48-49 1.252286358279072 1103 50-51 1.0364433019307242 1103 52-53 1.0122232733336176 1103 54-55 1.0003753801481352 1103 56-57 0.9037624212663005 1103 58-59 1.0369930858763396 1103 60-61 0.9359854776911689 1103 62-63 1.0285945931406602 1103 64-65 0.9734467498064419 1103 66-67 1.0140959701556618 1103 68-69 0.9077228170158946 1103 70-71 0.7229187762864804 1103 72-73 0.5887838946929946 1103 74-75 0.5875837829992641 1103 76-77 0.7314552527716494 1103 78-79 0.8221017493638918 1103 80-81 0.9108422199013582 1103 82-83 0.9923664928665978 1103 84-85 0.9308935617103842 1103 86-87 1.0656205700221442 1103 88-89 1.1101410564813499 1103 90-91 1.1083862044891326 1103 92-93 0.9646984945251056 1103 94-95 0.7099896629706102 1103 96-97 0.6802947514292548 1103 98-99 0.8551378410690695 1103 100-101 0.7740727340832798 1103 102-103 0.7735251906903429 1103 104-105 0.8678210260919812 1103 106-107 0.9052317148880249 1103 108-109 0.9571456482468115 1103 110-111 1.1647714115203591 1103 112-113 1.1605148427516916 1103 114-115 1.4655462837828246 1103 116-117 1.2685178353182316 1103 118-119 0.9793532465555188 1103 120-121 1.3513386387755837 1104 1 0.45528970437424476 1104 2 0.6503077529084251 1104 3 0.660126229553434 1104 4 0.7880484979013929 1104 5 0.8925017845546321 1104 6 0.7544231032822637 1104 7 0.6738644365412867 1104 8 0.8369734149740609 1104 9 0.8287863276008522 1104 10-11 1.0450092474691957 1104 12-13 0.9131301075308613 1104 14-15 1.1146708747738998 1104 16-17 0.934801133534485 1104 18-19 0.8025053278671592 1104 20-21 0.9224252544206735 1104 22-23 0.8804261373291169 1104 24-25 1.1570112917558433 1104 26-27 0.9927361811687732 1104 28-29 0.9467789680853116 1104 30-31 1.1361926670364255 1104 32-33 0.9499067624365551 1104 34-35 0.8077030011699691 1104 36-37 0.8315474966838181 1104 38-39 0.750681902838771 1104 40-41 0.6556353233374494 1104 42-43 0.7122345637260139 1104 44-45 0.7493327177013924 1104 46-47 0.942295833153068 1104 48-49 1.0908572047061895 1104 50-51 0.8126605549738599 1104 52-53 0.760965145188294 1104 54-55 1.0126647858716566 1104 56-57 0.9700735245440626 1104 58-59 0.9975964248846836 1104 60-61 1.0265625791339232 1104 62-63 1.003617030696752 1104 64-65 1.022358747086436 1104 66-67 0.8873397157650338 1104 68-69 0.943066655375496 1104 70-71 1.0026419755944822 1104 72-73 0.8620555153720453 1104 74-75 0.9070086440614133 1104 76-77 0.8674605927849974 1104 78-79 0.8631334144430554 1104 80-81 1.0999805052470712 1104 82-83 1.0272325175316581 1104 84-85 1.0962427250832931 1104 86-87 1.0730259010354715 1104 88-89 0.9195908926059424 1104 90-91 1.0794180070686394 1104 92-93 1.123343891138596 1104 94-95 1.1910849282087739 1104 96-97 1.2219682931131075 1104 98-99 1.2772070212420203 1104 100-101 1.1484239824613987 1104 102-103 1.2120687870493327 1104 104-105 1.2718514111679475 1104 106-107 1.16231204508885 1104 108-109 1.2994899416075434 1104 110-111 1.4134604707430078 1104 112-113 1.0936921756950255 1104 114-115 1.105915384705579 1104 116-117 1.2298036760328337 1104 118-119 0.9850373232657113 1104 120-121 1.0505181992244879 1105 1 0.7844161706144455 1105 2 0.8589480732620594 1105 3 0.9913387951131938 1105 4 1.1019546568164529 1105 5 1.2452336159306938 1105 6 1.2036817547735623 1105 7 1.0042047266777274 1105 8 1.079219451428152 1105 9 1.2973116514084921 1105 10-11 1.3951045643174638 1105 12-13 1.3239367354544669 1105 14-15 1.4811294879159007 1105 16-17 1.3101273808070246 1105 18-19 1.1226357582952815 1105 20-21 1.312083055379233 1105 22-23 1.1701514775721762 1105 24-25 1.3176516395018396 1105 26-27 1.3899872295685043 1105 28-29 1.477658716486495 1105 30-31 1.4969185731028958 1105 32-33 1.3366932877928832 1105 34-35 1.5260723169345312 1105 36-37 1.5555444441478343 1105 38-39 1.351021610887578 1105 40-41 1.5926611466276341 1105 42-43 1.704889987541435 1105 44-45 1.6488967083914048 1105 46-47 1.7521866574832998 1105 48-49 1.5848963706431007 1105 50-51 1.7558316827429508 1105 52-53 1.8034588256855386 1105 54-55 1.7497396586041916 1105 56-57 1.9908018360020279 1105 58-59 2.096657680190887 1105 60-61 1.886994873355249 1105 62-63 1.8892214045716358 1105 64-65 1.9656766807780954 1105 66-67 1.7100865444575 1105 68-69 1.6100712324674333 1105 70-71 1.6138399718434435 1105 72-73 1.5018646163120337 1105 74-75 1.6019369488424928 1105 76-77 1.8873784756295926 1105 78-79 1.8720603273499066 1105 80-81 1.7203316153652075 1105 82-83 2.0622076375658303 1105 84-85 1.852460790515078 1105 86-87 2.0031152514856068 1105 88-89 1.7768296230043532 1105 90-91 1.8021210935882621 1105 92-93 1.9791836852165403 1105 94-95 1.9843391746663883 1105 96-97 2.039083852058468 1105 98-99 2.0554889896226776 1105 100-101 2.0769746527609776 1105 102-103 2.215699558597919 1105 104-105 2.3134918624912935 1105 106-107 2.363337289715453 1105 108-109 2.3510483528164983 1105 110-111 2.589340172063576 1105 112-113 2.49040051298741 1105 114-115 2.4373430230819295 1105 116-117 2.4251111937800864 1105 118-119 2.617225356400194 1105 120-121 2.409758069829831 1106 1 0.6911680109960798 1106 2 0.7889485732458823 1106 3 0.8277899687516914 1106 4 1.0504427939649545 1106 5 1.0726503759766928 1106 6 1.0999915800439197 1106 7 1.0230670576023186 1106 8 0.9329083026712013 1106 9 1.0056634400270994 1106 10-11 0.9545844046755647 1106 12-13 1.177533824960392 1106 14-15 1.1646461976694056 1106 16-17 1.0175439055665194 1106 18-19 1.149216663030714 1106 20-21 1.2524808660383044 1106 22-23 1.1548031506062841 1106 24-25 1.5206033087125448 1106 26-27 1.4311247221790353 1106 28-29 1.4772481415345027 1106 30-31 1.2874221156353371 1106 32-33 1.38058569127395 1106 34-35 1.235797884636753 1106 36-37 1.3829065000813259 1106 38-39 1.3124731521612532 1106 40-41 1.418184732629257 1106 42-43 1.2084386962596838 1106 44-45 1.034735990061705 1106 46-47 1.2089259982693363 1106 48-49 1.551166285439983 1106 50-51 0.9675027757369961 1106 52-53 1.2919150815125562 1106 54-55 1.4537424702779305 1106 56-57 1.3922423776315682 1106 58-59 1.3872423965677072 1106 60-61 1.4556941213207537 1106 62-63 1.2799367049589776 1106 64-65 1.2071215163863513 1106 66-67 1.2027820748962164 1106 68-69 1.1103344592453936 1106 70-71 1.1043491024303869 1106 72-73 1.116511495383591 1106 74-75 1.1937328025060516 1106 76-77 1.3006012831269977 1106 78-79 1.1649529102369272 1106 80-81 1.1467345846809067 1106 82-83 1.2399418873401018 1106 84-85 1.3122332684643219 1106 86-87 1.1349321632913991 1106 88-89 1.0250436513740233 1106 90-91 1.1512100936500218 1106 92-93 1.1515689903978412 1106 94-95 0.9707357912464403 1106 96-97 1.204060867507966 1106 98-99 1.2061814672189897 1106 100-101 0.9407275901659453 1106 102-103 0.7382626521448898 1106 104-105 1.0205245761387935 1106 106-107 0.9873588654880017 1106 108-109 0.8623253409139409 1106 110-111 0.8964484715009498 1106 112-113 0.5082875813469663 1106 114-115 0.858009413286954 1106 116-117 1.3122824911792854 1106 118-119 1.6080974164217814 1106 120-121 1.5215100425601271 1107 1 0.43490869085486494 1107 2 0.5404186412872498 1107 3 0.46647228285172204 1107 4 0.7463457465856891 1107 5 0.7901566356436334 1107 6 0.7025883881339752 1107 7 0.7459923508182911 1107 8 0.7027072303781168 1107 9 0.7249231000283487 1107 10-11 0.8580877373846718 1107 12-13 0.808511373390445 1107 14-15 0.9774967216780865 1107 16-17 0.8783344616191471 1107 18-19 0.5865031234231495 1107 20-21 0.8391645950658173 1107 22-23 0.7842981463406957 1107 24-25 0.8251657562596577 1107 26-27 0.7870975888637375 1107 28-29 1.1399852632668228 1107 30-31 0.9942144611550816 1107 32-33 0.7636388134779111 1107 34-35 1.0290502757948374 1107 36-37 0.9440473200456765 1107 38-39 1.136531774549006 1107 40-41 1.0473440298819412 1107 42-43 0.9922897959496453 1107 44-45 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-1.1778653112195059 2316 98-99 -0.8562693512058956 2316 100-101 -0.6375037208355145 2316 102-103 -0.9025156666604062 2316 104-105 -1.2166653897329986 2316 106-107 -1.1882758757245249 2316 108-109 -1.3230734374266007 2316 110-111 -1.223842053030065 2316 112-113 -1.072381614140287 2316 114-115 -0.9944534695685157 2316 116-117 -1.3570787461313216 2316 118-119 -0.7973125568408328 2316 120-121 -0.8167385499229738 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16006.0 3 2228.0 4 1879.0 5 2274.0 6 2762.0 7 3021.0 8 3651.0 9 4603.0 10 6126.0 11 7650.0 12 8213.0 13 9212.0 14 11526.0 15 11813.0 16 9344.0 17 9339.0 18 9835.0 19 10711.0 20 11694.0 21 12802.0 22 14419.0 23 16096.0 24 17737.0 25 20464.0 26 23459.0 27 27487.0 28 31770.0 29 37367.0 30 44704.0 31 53219.0 32 64596.0 33 79770.0 34 101842.0 35 131512.0 36 172963.0 37 188763.0 38 66259.0 39 315.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.88818852315627 18.33194541070552 13.683922857836148 22.09594320830206 2 14.092498814511991 21.38838852933132 38.447844192079806 26.07126846407688 3 22.47770540494875 24.617630818806443 30.364143031418177 22.54052074482663 4 11.261415645071853 15.565451315072613 42.03067128600505 31.14246175385048 5 11.065220255894738 37.9478299392155 36.05719676667532 14.929753038214443 6 27.10500914325073 36.50008326744695 18.88481444294078 17.510093146361534 7 24.80153838568118 32.53355923051617 24.04301530706686 18.621887076735796 8 22.728294133147532 30.254681308875046 21.701744487751725 25.315280070225693 9 21.673507857804537 19.789768768278428 26.120470708439157 32.41625266547788 10-11 23.966010886733674 24.014915504137896 28.856513044195225 23.16256056493321 12-13 27.301031447028954 22.745025760913983 27.933949234811735 22.01999355724533 14-15 22.53370777887988 24.75920127984065 30.11586632164646 22.591224619633007 16-17 19.732491223985242 28.443261912500184 29.906685617360907 21.91756124615367 18-19 18.636276714751162 30.75141106119969 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19.389919694765677 27.77682490934715 28.76251785954108 24.070737536346087 70-71 22.470022563531277 26.652452802387344 27.744885559097103 23.132639074984272 72-73 21.14923981655365 25.159345639465336 31.40326581139872 22.288148732582293 74-75 19.34146899333858 29.525604253915176 29.267325049237 21.86560170350924 76-77 20.021326717786554 26.977569296651488 28.525618578048785 24.475485407513176 78-79 20.815857189196972 26.96412985182926 29.40251203477729 22.817500924196473 80-81 22.634584441943865 26.29625524913098 28.210939225357297 22.858221083567866 82-83 20.954304815776016 25.381359497259133 31.315852064227606 22.348483622737245 84-85 20.558343507594603 26.944813917176187 29.83201246550895 22.664830109720256 86-87 20.816945015322723 28.06064223604193 29.07087436984339 22.05153837879196 88-89 19.60471891464316 27.046367396589737 27.636595690989708 25.712317997777394 90-91 19.772191749463502 24.984165939337956 28.737424762933983 26.50621754826456 92-93 21.576725392682967 26.551309536375474 29.595631279398898 22.276333791542662 94-95 20.39285896273457 25.89192192806712 29.93439544076218 23.780823668436128 96-97 17.16791224808471 27.082832006865626 32.24188073559608 23.507375009453586 98-99 19.138900551641456 28.30388252358775 28.523964731836237 24.033252192934558 100-101 17.786898904871535 26.143826009017946 30.233610688220857 25.835664397889662 102-103 20.001213229049554 24.734909452671936 29.598987358153305 25.664889960125205 104-105 22.258425743979675 25.377140987621473 29.059843922694945 23.30458934570391 106-107 20.638157189681912 25.891751878984692 30.871966175242495 22.598124756090904 108-109 19.631085818185348 26.365057693645365 30.179325082217286 23.824531405951998 110-111 19.226790448782964 28.26472905272387 28.10146833542818 24.40701216306498 112-113 20.541009668484268 25.65672856863807 26.670247137409596 27.132014625468067 114-115 19.166762378816284 25.610144732946928 28.469942880067556 26.753150008169236 116-117 22.070397025764894 25.0311231765944 27.20460889957022 25.693870898070486 118-119 20.85408233592514 23.902244289777418 30.15909995437646 25.084573419920986 120-121 19.222500113241487 27.41843377314171 27.92563431411248 25.433431799504326 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2984.0 1 3082.5 2 2004.5 3 845.5 4 812.5 5 718.0 6 704.5 7 772.5 8 867.5 9 909.5 10 847.5 11 835.5 12 919.5 13 1034.5 14 1077.5 15 1117.0 16 1246.5 17 1416.0 18 1530.5 19 1653.5 20 1875.0 21 2093.0 22 2453.5 23 2998.5 24 3623.0 25 4507.0 26 5696.5 27 7281.0 28 9547.0 29 12203.0 30 15054.0 31 17671.5 32 20159.5 33 22765.0 34 25277.5 35 27610.5 36 29871.0 37 32720.5 38 35306.5 39 36732.0 40 37918.5 41 39126.0 42 40266.0 43 41448.5 44 42169.0 45 43636.5 46 48541.0 47 70897.0 48 119534.0 49 118430.0 50 70352.0 51 45871.0 52 34211.5 53 28029.0 54 24176.5 55 20749.5 56 17643.5 57 14529.0 58 11954.0 59 9946.0 60 8129.5 61 6266.0 62 4646.0 63 3323.5 64 2208.5 65 1475.0 66 946.5 67 579.0 68 334.5 69 209.5 70 139.0 71 93.5 72 66.0 73 46.5 74 42.5 75 35.5 76 20.0 77 14.0 78 16.0 79 14.5 80 12.0 81 10.5 82 6.0 83 3.0 84 2.5 85 5.5 86 6.5 87 3.0 88 0.5 89 0.5 90 1.5 91 2.5 92 2.0 93 0.5 94 1.0 95 2.0 96 2.0 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5585880100783129 2 0.9341598853964668 3 0.8394853102095426 4 0.8267391142275605 5 0.8367597085530181 6 0.837962179872073 7 0.8248953248716763 8 0.8238531830618286 9 0.8270597732459752 10-11 0.828021750301219 12-13 0.832310564672515 14-15 0.8338737773872864 16-17 0.8446960192587807 18-19 0.8565203205628208 20-21 0.8548769430934456 22-23 0.8438142069581404 24-25 0.8461790672189483 26-27 0.84818318608404 28-29 0.8561194967898024 30-31 0.8628533361765099 32-33 0.8669417386612968 34-35 0.8717516239375164 36-37 0.8754392026492848 38-39 0.8678235509619371 40-41 0.8712305530325926 42-43 0.8661801734925619 44-45 0.8677033038300315 46-47 0.8669417386612968 48-49 0.8715912944283091 50-51 0.9586903003051872 52-53 0.8800887584162972 54-55 0.8806499116985228 56-57 0.9077856811318622 58-59 0.8646971255323942 60-61 0.8849788084471205 62-63 0.8984865695978376 64-65 0.8939973433400324 66-67 0.8991679700119685 68-69 0.9136377082179296 70-71 0.98217857340406 72-73 0.9861467287569413 74-75 0.9887120009042584 76-77 0.9908764492785573 78-79 0.8996088761622887 80-81 0.9037774434016791 82-83 0.9080261753956731 84-85 0.8865821035391938 86-87 0.8843775727875931 88-89 0.8854197145974407 90-91 0.8956006384321057 92-93 0.8895481994595291 94-95 0.8885461400269835 96-97 0.8920333068522428 98-99 0.9060220565305817 100-101 0.9001700294445143 102-103 0.8867023506710993 104-105 0.8863416092753827 106-107 0.8865019387845902 108-109 0.8876643277263431 110-111 0.887023009689514 112-113 0.8893878699503218 114-115 0.8886263047815871 116-117 0.8989675581254594 118-119 0.900009699935307 120-121 0.8926345425117702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1247431.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.12120033191233 #Duplication Level Percentage of deduplicated Percentage of total 1 86.05762069871315 51.73887454115058 2 8.99239331659295 10.812669601004686 3 2.088631566068893 3.7671311040955104 4 0.855698312052508 2.0578243857035226 5 0.48286804202702976 1.4515303144292662 6 0.3200329338120112 1.1544458475912938 7 0.2136765568562402 0.8992543756691088 8 0.15501770506676635 0.7455880401049888 9 0.12489204746566719 0.6757793824991473 >10 0.6436723286906518 6.904215899543488 >50 0.037825117258658864 1.5717437240364518 >100 0.020247353963701902 2.333634932071858 >500 0.002564661949168584 1.001183618385653 >1k 0.0037795018198273876 5.6071801028401005 >5k 5.399288314039125E-4 2.317321733241657 >10k+ 5.399288314039125E-4 6.961622397632719 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 27294 2.188016812152336 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 25315 2.0293707627916895 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 18148 1.454829966547248 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 15027 1.2046357674292205 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9638 0.772627904870089 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6883 0.5517740059370017 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6873 0.5509723583909651 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5161 0.4137302985094967 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4634 0.37148347283336713 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4608 0.36939918921367193 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4107 0.32923664715723755 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3999 0.3205788536600421 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3928 0.3148871560831822 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3872 0.3103979298253771 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3412 0.2735221427076928 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3357 0.26911308120449146 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3331 0.26702879758479625 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3198 0.2563668852225093 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2532 0.20297715865647079 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2528 0.20265649963805613 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2219 0.17788559046552474 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2159 0.17307570518930507 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2078 0.16658236006640847 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1880 0.15070973865488352 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1875 0.1503089148818652 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1819 0.14581968862406017 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1663 0.133313986905889 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1547 0.12401487537186426 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1521 0.12193059175216905 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1514 0.12136943846994341 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1451 0.11631905892991276 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1285 0.10301170966570497 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.016475460366144E-5 0.0 10-11 0.0 0.0 0.0 1.2024713190549217E-4 0.0 12-13 0.0 0.0 0.0 3.206590184146458E-4 0.0 14-15 0.0 0.0 0.0 4.008237730183072E-4 0.0 16-17 0.0 0.0 0.0 4.008237730183072E-4 0.0 18-19 0.0 1.603295092073229E-4 0.0 8.417299233384452E-4 0.0 20-21 0.0 1.603295092073229E-4 0.0 0.0014028832055640753 0.0 22-23 0.0 1.603295092073229E-4 0.0 0.0023648602608080127 0.0 24-25 0.0 1.603295092073229E-4 0.0 0.0038479082209757496 0.0 26-27 0.0 1.603295092073229E-4 0.0 0.00557145044495447 0.0 28-29 0.0 1.603295092073229E-4 0.0 0.010661912362286972 0.0 30-31 0.0 1.603295092073229E-4 0.0 0.019119293972973253 0.0 32-33 0.0 1.603295092073229E-4 0.0 0.035993974817043986 0.0 34-35 0.0 1.603295092073229E-4 0.0 0.05274840852920923 0.0 36-37 0.0 1.603295092073229E-4 0.0 0.07282967955742642 0.0 38-39 0.0 1.603295092073229E-4 0.0 0.10188940310125369 0.0 40-41 0.0 1.603295092073229E-4 0.0 0.13575901192130066 0.0 42-43 0.0 1.603295092073229E-4 0.0 0.1834570409104792 0.0 44-45 0.0 1.603295092073229E-4 0.0 0.21776755588084631 0.0 46-47 0.0 1.603295092073229E-4 0.0 0.29071748257017827 0.0 48-49 0.0 1.603295092073229E-4 0.0 0.3667938346890529 0.0 50-51 0.0 1.603295092073229E-4 0.0 0.39597380536478566 0.0 52-53 0.0 1.603295092073229E-4 0.0 0.44936353193082423 0.0 54-55 0.0 1.603295092073229E-4 0.0 0.5561029026855995 0.0 56-57 0.0 1.603295092073229E-4 0.0 0.7184766131353157 0.0 58-59 0.0 1.603295092073229E-4 0.0 0.9094691409785391 0.0 60-61 0.0 1.603295092073229E-4 0.0 1.1148913246504215 0.0 62-63 0.0 1.603295092073229E-4 0.0 1.2926967503613427 0.0 64-65 0.0 1.603295092073229E-4 0.0 1.4741897547840321 0.0 66-67 0.0 1.603295092073229E-4 0.0 1.8782201179864857 0.0 68-69 0.0 1.603295092073229E-4 0.0 2.31050855718673 0.0 70-71 0.0 1.603295092073229E-4 0.0 2.4391329059483047 0.0 72-73 0.0 1.603295092073229E-4 0.0 2.6004644745881738 0.0 74-75 0.0 2.4049426381098435E-4 0.0 2.7809554195783175 0.0 76-77 0.0 2.4049426381098435E-4 0.0 2.926414366806661 0.0 78-79 0.0 2.4049426381098435E-4 0.0 3.104340039649488 0.0 80-81 0.0 3.206590184146458E-4 0.0 3.4450803290923506 0.0 82-83 0.0 3.206590184146458E-4 0.0 3.8980512749803395 0.0 84-85 0.0 3.206590184146458E-4 0.0 4.811889395084778 0.0 86-87 0.0 3.206590184146458E-4 0.0 6.098253129832432 0.0 88-89 0.0 3.206590184146458E-4 0.0 7.353272445530053 0.0 90-91 0.0 3.206590184146458E-4 0.0 8.473935632511939 0.0 92-93 0.0 3.206590184146458E-4 0.0 9.51876296163876 0.0 94-95 0.0 3.206590184146458E-4 0.0 10.457973226575259 0.0 96-97 0.0 3.206590184146458E-4 0.0 11.551580808878406 0.0 98-99 0.0 4.008237730183072E-4 0.0 12.667995263866299 0.0 100-101 0.0 4.008237730183072E-4 0.0 13.372122385927558 0.0 102-103 0.0 4.008237730183072E-4 0.0 13.94646277028549 0.0 104-105 0.0 4.008237730183072E-4 0.0 14.521083731284536 0.0 106-107 0.0 4.008237730183072E-4 0.0 15.278961321307552 0.0 108-109 0.0 4.008237730183072E-4 0.0 16.24230117737975 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 205 0.0 58.89121 2 TACCTGG 1795 0.0 54.76671 2 ACCTGGG 1930 0.0 49.967438 3 TAACGCA 260 0.0 44.156208 4 GTACCTG 2500 0.0 40.794693 1 GTACCCG 220 0.0 36.667038 1 GTACCGG 130 5.57593E-8 35.45823 1 ATAACGC 380 0.0 34.743965 3 GTATAAC 435 0.0 31.790138 1 GAGTACG 635 0.0 30.851452 1 TACGCAG 600 0.0 30.61497 4 CCTGGGG 1290 0.0 30.258984 4 AGTACGC 610 0.0 30.15818 2 GTACGCA 635 0.0 28.927534 3 TACCGCG 100 5.481413E-4 28.696898 8 GAGTACC 760 0.0 27.293509 1 TACCCGG 260 5.638867E-11 26.533398 2 TCACGCA 260 5.820766E-11 26.493725 4 TATCACG 265 7.2759576E-11 26.032768 2 ACGCAGT 735 0.0 25.77489 5 >>END_MODULE