##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003797_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1247431 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.667261756361675 31.0 31.0 34.0 30.0 34.0 2 31.877707865204567 31.0 31.0 34.0 30.0 34.0 3 31.935865791374432 31.0 31.0 34.0 30.0 34.0 4 35.621310517375306 37.0 35.0 37.0 33.0 37.0 5 35.527665257637494 37.0 35.0 37.0 33.0 37.0 6 35.58733108284146 37.0 35.0 37.0 33.0 37.0 7 35.52673614813164 37.0 35.0 37.0 33.0 37.0 8 35.52933829606607 37.0 35.0 37.0 33.0 37.0 9 37.095588453389404 39.0 37.0 39.0 33.0 39.0 10-11 37.01128599497687 39.0 37.0 39.0 33.0 39.0 12-13 36.96864876694583 39.0 37.0 39.0 33.0 39.0 14-15 37.97718871825376 40.0 37.0 41.0 33.0 41.0 16-17 38.068169301548544 40.0 37.0 41.0 33.0 41.0 18-19 38.10521143053203 40.0 37.0 41.0 33.0 41.0 20-21 38.02285777730391 40.0 37.0 41.0 33.0 41.0 22-23 37.925919349446986 40.0 37.0 41.0 33.0 41.0 24-25 37.786625071847666 39.5 37.0 41.0 32.5 41.0 26-27 37.49336396161391 39.0 36.5 41.0 32.0 41.0 28-29 37.37353729384631 39.0 36.0 41.0 32.0 41.0 30-31 37.20073895870794 39.0 36.0 40.0 31.0 41.0 32-33 36.942687811991206 39.0 35.5 40.0 31.0 41.0 34-35 36.84929026134512 39.0 35.0 40.0 30.0 41.0 36-37 36.63082687539431 38.5 35.0 40.0 30.0 41.0 38-39 36.397221569770196 38.0 35.0 40.0 30.0 41.0 40-41 36.67244039950907 39.0 35.0 40.0 30.0 41.0 42-43 36.551830522089 39.0 35.0 41.0 30.0 41.0 44-45 36.38689875431987 39.0 35.0 41.0 30.0 41.0 46-47 36.11212323567396 39.0 35.0 40.0 29.5 41.0 48-49 35.81562427100177 39.0 35.0 40.0 28.0 41.0 50-51 35.51077694878514 38.0 35.0 40.0 28.0 41.0 52-53 35.42139204493074 38.0 34.5 40.0 27.0 41.0 54-55 35.23212386095904 38.0 34.0 40.0 27.0 41.0 56-57 34.90812517886761 38.0 34.0 40.0 26.0 41.0 58-59 34.612617050562314 38.0 34.0 40.0 26.0 41.0 60-61 34.039402179359016 37.0 33.0 40.0 23.5 41.0 62-63 33.91123476969869 37.0 33.0 40.0 24.0 41.0 64-65 33.468104047438295 36.5 32.5 39.5 22.5 41.0 66-67 33.06825828442615 36.0 32.0 39.0 22.0 40.0 68-69 32.700328515164365 35.5 32.0 39.0 22.0 40.0 70-71 32.181521863734346 35.0 31.0 38.0 20.5 40.0 72-73 31.737202698986955 35.0 31.0 37.0 20.5 39.5 74-75 31.398276938764546 35.0 31.0 37.0 19.5 39.0 76-77 30.45872396950212 34.0 29.5 36.0 18.0 38.5 78-79 30.832633227809794 34.0 30.5 36.0 18.0 38.5 80-81 30.54517404169048 34.0 30.0 35.5 17.0 37.0 82-83 30.212940836006158 34.0 30.0 35.0 14.0 37.0 84-85 29.88976624759205 34.0 30.0 35.0 9.5 36.5 86-87 29.684388956182744 34.0 30.0 35.0 7.0 36.0 88-89 29.371826978806844 34.0 29.0 35.0 4.5 36.0 90-91 29.01813887902417 34.0 29.0 35.0 2.0 35.5 92-93 28.755234558063734 34.0 29.0 35.0 2.0 35.0 94-95 28.372218182809313 34.0 29.0 35.0 2.0 35.0 96-97 27.930807796182716 34.0 28.5 35.0 2.0 35.0 98-99 27.62093694961886 34.0 29.0 35.0 2.0 35.0 100-101 26.584482829110385 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0610118603902805 1101 2 0.1394662291272546 1101 3 0.18596889663447058 1101 4 0.13333830148103942 1101 5 0.09658852426722575 1101 6 0.11000746091745839 1101 7 0.10648462370503609 1101 8 0.16853942445874992 1101 9 0.2330010343531299 1101 10-11 0.2924760450506483 1101 12-13 0.25358648153385843 1101 14-15 0.23739403857139507 1101 16-17 0.08768238351141378 1101 18-19 0.09076938977926119 1101 20-21 0.10585815879293392 1101 22-23 0.1687603045589654 1101 24-25 0.193949947390756 1101 26-27 0.3185625815175328 1101 28-29 0.10176774726065929 1101 30-31 0.21765882895804367 1101 32-33 0.11888819019100083 1101 34-35 0.08726947709607202 1101 36-37 0.15622901646339216 1101 38-39 0.15426260282442428 1101 40-41 0.1932124443655141 1101 42-43 0.11414670894715329 1101 44-45 0.25469372079341923 1101 46-47 0.22235525442618354 1101 48-49 0.19484361482547286 1101 50-51 0.14030765009093926 1101 52-53 0.21834750363522204 1101 54-55 0.28472815570130194 1101 56-57 0.1251319860576885 1101 58-59 -0.13986479735066126 1101 60-61 0.07881249624152531 1101 62-63 -0.04270705806414554 1101 64-65 -0.22583266901486354 1101 66-67 -0.047630853028273634 1101 68-69 -0.11770009105082835 1101 70-71 -0.10932108039730082 1101 72-73 -0.21165681450815654 1101 74-75 -0.20518498356055304 1101 76-77 -0.1066320414504709 1101 78-79 -0.04802498240735531 1101 80-81 0.029777788424478757 1101 82-83 0.09403447839870083 1101 84-85 -0.13668200898790417 1101 86-87 -0.10126214366311004 1101 88-89 0.02313636621501658 1101 90-91 -0.013506857557448626 1101 92-93 0.22281728213644314 1101 94-95 0.10317738153882416 1101 96-97 0.20428220727542978 1101 98-99 -0.26119926886641665 1101 100-101 -0.04494836425502413 1102 1 -0.0208409415287214 1102 2 -0.03699025200712214 1102 3 -0.04562424956776567 1102 4 -0.04007935925477568 1102 5 0.019537593331769187 1102 6 -0.05088277322285961 1102 7 -0.09004476947488627 1102 8 -0.12352019076951137 1102 9 -0.10657145725587469 1102 10-11 -0.037505937019822966 1102 12-13 0.050983540060485666 1102 14-15 0.13415162975647377 1102 16-17 0.0873409322877805 1102 18-19 -0.18924830620664324 1102 20-21 -0.14911218692540018 1102 22-23 -0.16360624298856408 1102 24-25 -0.0046645764137096535 1102 26-27 -0.10183003691542325 1102 28-29 0.029577755778525727 1102 30-31 0.021631313145974218 1102 32-33 0.025987265339729504 1102 34-35 0.0025206831569093424 1102 36-37 -0.16892613035108184 1102 38-39 -0.03609011096933301 1102 40-41 0.07546893084742834 1102 42-43 0.07954963776105473 1102 44-45 0.29252564330431596 1102 46-47 0.3139396208438612 1102 48-49 0.24289820227654246 1102 50-51 0.27634615563955833 1102 52-53 0.308861543924003 1102 54-55 0.29490525743384666 1102 56-57 0.370182102857747 1102 58-59 0.2848070793460238 1102 60-61 0.20484332970990948 1102 62-63 0.016162379609475863 1102 64-65 0.07419518729351182 1102 66-67 -0.018594130174264478 1102 68-69 0.03176387281727244 1102 70-71 0.02121396262037223 1102 72-73 0.17559470913096575 1102 74-75 0.17537420522882385 1102 76-77 0.2209491415450131 1102 78-79 0.2562998663473479 1102 80-81 0.23386917348596725 1102 82-83 0.19399278942788456 1102 84-85 0.13986606237881105 1102 86-87 0.09591331943096648 1102 88-89 0.25382697893921247 1102 90-91 0.304629099780108 1102 92-93 0.2889360444105442 1102 94-95 0.31464986981925946 1102 96-97 0.44094651120330397 1102 98-99 0.3451430750686839 1102 100-101 0.3124277461094316 1103 1 0.03659068486776107 1103 2 -0.12138274787818304 1103 3 -0.14084695745664533 1103 4 -0.0019454542400509922 1103 5 0.07433955483824661 1103 6 -0.01000628002952908 1103 7 0.04659183064232053 1103 8 -0.030976370742600068 1103 9 -0.13253570632179645 1103 10-11 0.01678236829695834 1103 12-13 -0.02952265740123039 1103 14-15 0.033732713310442364 1103 16-17 -0.09290258823182995 1103 18-19 -0.06519164998682925 1103 20-21 -0.056573868289468976 1103 22-23 -0.12550216130390623 1103 24-25 -0.10307577469773577 1103 26-27 -0.09957417706538507 1103 28-29 -0.1800770695783669 1103 30-31 -0.06984465793639316 1103 32-33 -0.11027610883130023 1103 34-35 -0.018592207702440078 1103 36-37 -0.08730344006665547 1103 38-39 -0.14862540673568958 1103 40-41 0.023574599249059247 1103 42-43 -0.0381083216514142 1103 44-45 0.042170948359341764 1103 46-47 0.10753698619876673 1103 48-49 0.23361185429677533 1103 50-51 0.18464028432346424 1103 52-53 0.21783437052711463 1103 54-55 0.18179115742666596 1103 56-57 0.2109900892559935 1103 58-59 0.07916942759450052 1103 60-61 0.22179195714181787 1103 62-63 -0.0172307741121287 1103 64-65 -0.04422659682495578 1103 66-67 -0.13930130792594753 1103 68-69 -0.12451761540743433 1103 70-71 -0.09436273371906267 1103 72-73 0.19979156395678643 1103 74-75 0.0532192930078601 1103 76-77 0.21163962981373174 1103 78-79 0.09121962711212461 1103 80-81 0.04338959117473706 1103 82-83 0.18091540396684636 1103 84-85 0.13963500395751538 1103 86-87 -0.05532252462139198 1103 88-89 -0.09628675508069406 1103 90-91 -0.09903951727443427 1103 92-93 -0.1534169063039421 1103 94-95 -0.24668340097194275 1103 96-97 -0.07264221407228533 1103 98-99 -0.1701258908319545 1103 100-101 -0.23511411658047976 1104 1 0.03410505365368266 1104 2 -0.0333800278713845 1104 3 0.0049884071317656264 1104 4 -0.009418597922909555 1104 5 0.038501965244272185 1104 6 -0.08788459972532792 1104 7 -0.03623900143475822 1104 8 -0.0826264998679207 1104 9 -0.04227548067123621 1104 10-11 0.023341134693872334 1104 12-13 -0.15837172952391398 1104 14-15 -0.10985014564670337 1104 16-17 -0.04129558421431767 1104 18-19 -0.142311530286527 1104 20-21 -0.1156905785812441 1104 22-23 -0.11608182525306887 1104 24-25 0.17037365892584688 1104 26-27 -0.011716144920306704 1104 28-29 3.6619402978743665E-4 1104 30-31 0.02562745574388714 1104 32-33 0.0251782923047017 1104 34-35 0.027400094778315065 1104 36-37 0.11818769866119538 1104 38-39 -0.06897458260862521 1104 40-41 -0.13920393269727072 1104 42-43 0.04360188262409537 1104 44-45 0.2057929199142663 1104 46-47 0.14139425834194697 1104 48-49 0.15465478190409243 1104 50-51 0.1715658766374446 1104 52-53 0.26178573040551356 1104 54-55 0.09318249840502091 1104 56-57 -0.013440159319635825 1104 58-59 0.0913710205984799 1104 60-61 0.30497060258842623 1104 62-63 0.056094096700043394 1104 64-65 0.0011653916550145027 1104 66-67 0.10290898509978774 1104 68-69 0.0722238139461382 1104 70-71 0.15395476207467595 1104 72-73 0.19037904042547638 1104 74-75 0.08839281844167246 1104 76-77 0.07896679813164909 1104 78-79 0.19044268766977623 1104 80-81 0.12230041345178932 1104 82-83 0.07678795614822675 1104 84-85 0.1581450502326298 1104 86-87 0.07106747885361742 1104 88-89 0.08451869693293546 1104 90-91 0.37286447498554764 1104 92-93 0.3454943409741773 1104 94-95 0.13208785845620596 1104 96-97 0.2615701839587139 1104 98-99 0.2598051839957307 1104 100-101 0.2888715683837333 1105 1 -0.005630986729499909 1105 2 0.09955551705251153 1105 3 0.10705190775536977 1105 4 0.1308289947595611 1105 5 0.05246222406782408 1105 6 0.07603512696741177 1105 7 0.0069330679466617084 1105 8 0.1063645111583611 1105 9 0.09636409495264786 1105 10-11 0.11772193470483217 1105 12-13 -0.054124330299970325 1105 14-15 -0.03656081984969717 1105 16-17 0.030860382740222292 1105 18-19 0.06612677142147305 1105 20-21 0.12206075701024588 1105 22-23 0.02755343179425296 1105 24-25 0.1252971786042636 1105 26-27 0.23799709132423885 1105 28-29 0.34235401470228766 1105 30-31 0.16496810454902544 1105 32-33 0.21156981128804375 1105 34-35 0.3308237544145527 1105 36-37 0.16572499720965794 1105 38-39 0.1316151663147238 1105 40-41 0.25008043337079044 1105 42-43 0.24747957060629489 1105 44-45 0.22548588581334883 1105 46-47 0.32681399059876526 1105 48-49 0.47467671291129676 1105 50-51 0.6464494377125618 1105 52-53 0.676857862190154 1105 54-55 0.7279291490471564 1105 56-57 0.7300323271960494 1105 58-59 0.6142260919084919 1105 60-61 0.7283550312309046 1105 62-63 0.7864507794511653 1105 64-65 0.5127230410139916 1105 66-67 0.614962906957949 1105 68-69 0.621617372804188 1105 70-71 0.7848977269037505 1105 72-73 0.7516103783110708 1105 74-75 0.6968642049829477 1105 76-77 0.7334969378583587 1105 78-79 0.7590932887180486 1105 80-81 1.0473140454303156 1105 82-83 1.0635478167553636 1105 84-85 1.016640405087795 1105 86-87 0.9950590334470171 1105 88-89 0.8188006190889858 1105 90-91 1.057267265924498 1105 92-93 0.9639100097457742 1105 94-95 1.1750033384773317 1105 96-97 1.0177097103619488 1105 98-99 1.0842991315684678 1105 100-101 1.283797347931742 1106 1 -0.11611329465483422 1106 2 -0.03000146786856206 1106 3 0.030246745569442623 1106 4 0.0759919453699851 1106 5 0.1713383780746085 1106 6 0.17395666480000926 1106 7 0.049160525293629576 1106 8 0.09215261801372776 1106 9 -0.049748236707209514 1106 10-11 -0.012093865372229118 1106 12-13 -0.08142609406644397 1106 14-15 -0.0188046296087947 1106 16-17 0.13290031674236502 1106 18-19 -0.03353176903751631 1106 20-21 0.15293622868616552 1106 22-23 0.061823793635490176 1106 24-25 0.0911885762355169 1106 26-27 0.15126136806822643 1106 28-29 0.2662652999254149 1106 30-31 0.01848519664310544 1106 32-33 0.024032349264750508 1106 34-35 0.1905468221976605 1106 36-37 0.0983318834574547 1106 38-39 0.24180071913507817 1106 40-41 -0.03743114713689977 1106 42-43 -0.05362657007739102 1106 44-45 0.2105269580139577 1106 46-47 0.09885577748212881 1106 48-49 -0.025310934547164265 1106 50-51 0.20357758944882676 1106 52-53 0.33522126807996244 1106 54-55 0.3320451923516359 1106 56-57 0.22521454188910894 1106 58-59 0.23760798249438864 1106 60-61 0.23230402111652637 1106 62-63 0.24836112941043353 1106 64-65 0.19052228868539345 1106 66-67 0.1664212421294593 1106 68-69 0.2899842785807607 1106 70-71 0.3240626362743271 1106 72-73 0.5001626310323104 1106 74-75 0.5628685401368152 1106 76-77 0.4036170222085751 1106 78-79 0.34041801056527987 1106 80-81 0.46565962836734087 1106 82-83 0.4715169706944984 1106 84-85 0.4125831834096765 1106 86-87 0.6135643170996019 1106 88-89 0.6034405277978259 1106 90-91 0.5654005322011741 1106 92-93 0.6185035376022263 1106 94-95 0.6102713151016168 1106 96-97 0.8340565344352875 1106 98-99 0.6604884313264172 1106 100-101 0.5292497009438506 1107 1 -0.15132764923288633 1107 2 -0.05704045466950802 1107 3 -0.16296923201366909 1107 4 -0.22383812461343666 1107 5 -0.17373330464871373 1107 6 -0.06437053024496464 1107 7 -0.17906658443954626 1107 8 -0.06770713770320214 1107 9 -0.09065212567701764 1107 10-11 -0.1345173448453778 1107 12-13 -0.09165449388055436 1107 14-15 -0.011285052977292764 1107 16-17 -0.12225237751538742 1107 18-19 -0.12348543709830295 1107 20-21 -0.19293898025839695 1107 22-23 -0.2163714108700816 1107 24-25 -0.1630797883441346 1107 26-27 0.05136991520125633 1107 28-29 -0.27950482984562797 1107 30-31 -0.07291391694644034 1107 32-33 -0.03346052407842848 1107 34-35 -0.10920175073700022 1107 36-37 -0.04678626992345869 1107 38-39 -0.03235951446668395 1107 40-41 0.08284081024881118 1107 42-43 0.06840086841835102 1107 44-45 0.05852213061429978 1107 46-47 0.016965808901836965 1107 48-49 3.5341211109596316E-5 1107 50-51 0.008756986994896465 1107 52-53 -0.28637995819052975 1107 54-55 -0.23776622000955427 1107 56-57 -0.2829112340668374 1107 58-59 0.10164732302720125 1107 60-61 -0.08132540660079712 1107 62-63 -0.21876746946455938 1107 64-65 -0.24615727879069738 1107 66-67 0.05156380994540655 1107 68-69 0.02493021309181387 1107 70-71 0.12794605734703168 1107 72-73 0.10590765277641623 1107 74-75 0.14746767869566568 1107 76-77 -0.0864916636428461 1107 78-79 -0.04031435312656839 1107 80-81 0.23621283727028697 1107 82-83 0.18819161366806725 1107 84-85 0.10383386833168018 1107 86-87 0.3467017107199446 1107 88-89 0.04412003978655932 1107 90-91 0.32764208251780147 1107 92-93 0.46793687301633824 1107 94-95 0.6424883122648275 1107 96-97 0.8491816930944331 1107 98-99 0.9032913749304612 1107 100-101 0.9559561104266372 1108 1 -0.22019986569070227 1108 2 -0.2521823920414725 1108 3 -0.13491964039954496 1108 4 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Count 2 1.0 3 2.0 4 6.0 5 18.0 6 74.0 7 175.0 8 432.0 9 748.0 10 1367.0 11 2240.0 12 3510.0 13 5038.0 14 5604.0 15 7005.0 16 8667.0 17 11256.0 18 10126.0 19 7634.0 20 7056.0 21 7324.0 22 7762.0 23 8622.0 24 10049.0 25 12291.0 26 14828.0 27 18711.0 28 23531.0 29 29282.0 30 36448.0 31 44749.0 32 54992.0 33 69550.0 34 90420.0 35 121149.0 36 172395.0 37 223394.0 38 193767.0 39 37192.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.398223074997915 19.34307037752437 13.473731157471876 19.78497539000584 2 15.134298804317764 24.998617129160134 37.13709661256769 22.729987453954408 3 25.25906299468963 25.9587455913982 28.60310474629389 20.179086667618286 4 12.590355699032651 16.86017102348747 40.80017251455191 29.749300762927973 5 12.102713496778579 40.239580385608505 33.45002649445139 14.207679623161521 6 27.91149027866517 39.31719377174003 17.229285295704464 15.542030653890338 7 27.99826202812019 33.41964405245661 21.473331991909774 17.108761927513424 8 24.658117362804035 31.060956477753077 19.7048173405984 24.57610881884449 9 23.295557028805604 22.316023892303463 23.300928067364048 31.08749101152689 10-11 25.56159643938799 25.545483259127195 27.824736697808127 21.06818360367669 12-13 29.729900892313882 23.650446397436014 26.284900727976137 20.334751982273968 14-15 22.645483770111994 26.952436687804138 29.21456000128268 21.18751954080119 16-17 20.057301766590697 29.140569698845063 29.11531780114491 21.686810733419325 18-19 19.691910815107207 32.2503208594303 28.642826737510934 19.41494158795156 20-21 22.680273925047032 27.051962457372085 27.42573713666307 22.84202648091781 22-23 20.58085777890721 28.493519882061612 28.00475537324309 22.920866965788086 24-25 24.02389390675717 28.03666094557535 27.930763304743913 20.008681842923576 26-27 23.24921292447362 25.4864555666102 31.740128385306644 19.52420312360954 28-29 20.370692544138848 31.430337509239852 28.98668659214921 19.21228335447209 30-31 22.30176202624199 27.66456561860125 27.76694561273264 22.26672674242412 32-33 22.33036133383626 28.52029218404083 28.734022813700406 20.415323668422502 34-35 24.21802815343167 26.400746867242265 29.61954746226681 19.761677517059255 36-37 23.54641233492892 25.947890858650247 31.083756284126718 19.42194052229412 38-39 21.25310016038457 28.706753589411495 30.90700060008923 19.133145650114706 40-41 21.074013081411664 29.516157740209387 28.416272070272097 20.99355710810685 42-43 20.281602749971743 29.994043758732946 26.74717078539815 22.97718270589716 44-45 22.841944764880783 26.37961538554036 27.542405151066475 23.236034698512384 46-47 24.4627353839494 29.320242418412256 27.094746799419607 19.12227539821874 48-49 23.625274664490462 28.701146596485096 27.944230983517325 19.729347755507117 50-51 19.831357405740277 29.211275012405498 31.08616829307593 19.871199288778296 52-53 22.350775313424148 31.14829597789377 26.19463521429241 20.306293494389667 54-55 20.521816437141613 28.849090651106152 27.52304536282969 23.106047548922547 56-57 23.42634876518096 26.976345778992382 27.979985722411126 21.61731973341553 58-59 26.303150841911982 26.884146065285506 26.378064955225888 20.43463813757662 60-61 22.20582275507574 28.513693844574473 30.341236745193033 18.93924665515675 62-63 20.792420548286774 28.29406213745734 29.563076710920754 21.350440603335134 64-65 20.356159505433382 32.195272968466405 27.3350234875833 20.11354403851691 66-67 22.439925381062938 27.277366992305975 26.39723077626226 23.885476850368825 68-69 21.212317983152317 29.240259032300475 26.8935203445213 22.653902640025912 70-71 24.248214328659724 28.643851759216947 26.386891448818773 20.721042463304553 72-73 23.224490973849456 26.335003699603426 29.98811156689228 20.452393759654843 74-75 20.756338426734626 31.868055227102744 27.340550298974453 20.03505604718818 76-77 22.434908223380692 28.123800033829525 26.28037141934103 23.160920323448753 78-79 22.56573710289387 29.018759354224805 27.08670860352196 21.328794939359373 80-81 24.02349308298415 27.63146819343114 27.39237681282572 20.95266191075899 82-83 22.875974703210037 26.837716875722982 29.416536866568173 20.869771554498804 84-85 20.798473341801408 28.717191057938702 29.02486350942998 21.45947209082991 86-87 21.469773497132287 29.135614328253396 26.65381885141654 22.740793323197774 88-89 20.66515101837296 29.330119261105423 25.87946748156812 24.125262238953496 90-91 22.529342304303803 25.492752705360054 27.19773678864803 24.78016820168811 92-93 23.299725596044993 28.449790008425314 27.37814756888357 20.872336826646123 94-95 22.110837570715447 28.578299847125564 28.015822944632518 21.295039637526465 96-97 18.77684029099946 29.078742208950437 30.68709541655811 21.457322083491995 98-99 21.18427961600898 30.913882598152192 25.933623263923682 21.968214521915144 100-101 19.051591069695704 28.762028369857518 26.963750250016933 25.222630310429846 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 168.0 1 114.5 2 193.5 3 340.0 4 272.0 5 203.0 6 282.5 7 499.5 8 718.0 9 831.5 10 864.0 11 881.5 12 959.5 13 1063.0 14 1188.0 15 1286.5 16 1398.5 17 1551.5 18 1690.0 19 1827.0 20 2040.5 21 2296.5 22 2600.0 23 3070.5 24 3679.5 25 4470.0 26 5612.5 27 7218.0 28 9507.0 29 12106.5 30 14733.0 31 17302.0 32 19871.5 33 22348.5 34 24700.5 35 27344.0 36 29748.5 37 32072.0 38 34674.5 39 36398.5 40 37625.5 41 38820.5 42 39899.0 43 41524.0 44 42230.5 45 43234.0 46 47387.5 47 71804.0 48 127373.0 49 125561.0 50 71605.0 51 46040.0 52 34364.0 53 28041.5 54 24018.0 55 20578.0 56 17325.0 57 14197.0 58 11663.5 59 9553.0 60 7677.5 61 6013.0 62 4457.5 63 3070.5 64 2044.0 65 1364.5 66 840.0 67 479.5 68 273.5 69 144.0 70 74.0 71 40.5 72 21.0 73 14.5 74 10.5 75 4.5 76 3.5 77 3.5 78 4.5 79 3.0 80 0.5 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06276900285466691 2 0.0020842836196951976 3 0.035031997761800056 4 0.0 5 0.0 6 0.0016032950920732288 7 0.0 8 0.0 9 0.0 10-11 4.008237730183072E-4 12-13 0.0 14-15 0.003286754938750119 16-17 0.0 18-19 0.0 20-21 0.012465619340869356 22-23 0.0 24-25 0.0 26-27 0.007375157423536853 28-29 0.00937927628862839 30-31 0.00937927628862839 32-33 0.005330956181143486 34-35 0.007014416027820377 36-37 0.01931970585948241 38-39 0.009339193911326558 40-41 0.013227184509604138 42-43 0.0 44-45 0.0 46-47 8.016475460366144E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0017235422239787212 58-59 2.004118865091536E-4 60-61 0.008056557837667974 62-63 0.013147019755000476 64-65 0.04825918227140419 66-67 0.023608520230778297 68-69 0.0015231303374695674 70-71 8.016475460366144E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 4.008237730183072E-5 86-87 6.012356595274608E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.603295092073229E-4 96-97 4.8098852762196864E-4 98-99 4.8098852762196864E-4 100-101 0.0012425536963567524 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1247431.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.89188506407532 #Duplication Level Percentage of deduplicated Percentage of total 1 84.44431285500744 48.041961412619024 2 9.997701621059623 11.375761830604873 3 2.3296590728782753 3.9761608863801334 4 0.9812687910744029 2.233049251150763 5 0.5478817749146268 1.5585013483572268 6 0.3465716527992261 1.1830268782512114 7 0.2330419885086785 0.9280738617737508 8 0.171806891511319 0.7819534340062415 9 0.12830436001408563 0.6569529212826991 >10 0.7421414579406937 7.633829713800274 >50 0.04402860774783818 1.7432260426262152 >100 0.02566580373021647 2.742081188950874 >500 0.0025383742711750355 1.0372857494453296 >1k 0.0035255198210764377 4.717327519211578 >5k 8.461247570583451E-4 3.606631880312889 >10k+ 7.051039642152876E-4 7.784176081226982 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 49256 3.9485951527579477 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14029 1.1246313423347662 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11656 0.9344003796602779 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11395 0.9134773787087223 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10688 0.8568008972039335 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8761 0.7023234150826779 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8404 0.6737045976891708 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8355 0.6697765247135914 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6885 0.5519343354462091 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6747 0.5408715993109038 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5802 0.46511590621044374 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4845 0.38839823605473967 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4077 0.3268317045191277 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3915 0.31384501427333455 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3810 0.30542771503995014 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3412 0.2735221427076928 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3153 0.25275947126534454 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3090 0.24770909172531388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2832 0.22702658503756923 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2784 0.22317867681659345 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2527 0.20257633488345245 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2234 0.17908806178457967 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2232 0.17892773227537234 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2170 0.17395751748994534 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2080 0.1667426895756158 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1968 0.15776423706000572 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1770 0.14189161564848077 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1666 0.13355448116969998 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1621 0.12994706721253518 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1499 0.1201669671508885 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1362 0.10918439577018689 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1250 0.10020594325457681 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.603295092073229E-4 0.0 10-11 0.0 0.0 0.0 2.0041188650915363E-4 0.0 12-13 0.0 0.0 0.0 3.607413957164765E-4 0.0 14-15 0.0 0.0 0.0 4.809885276219687E-4 0.0 16-17 0.0 0.0 0.0 4.809885276219687E-4 0.0 18-19 0.0 0.0 0.0 7.615651687347838E-4 0.0 20-21 0.0 4.008237730183072E-5 0.0 0.0013227184509604138 0.0 22-23 0.0 8.016475460366144E-5 0.0 0.0022846955062043512 0.0 24-25 0.0 8.016475460366144E-5 0.0 0.003767743466372088 0.0 26-27 0.0 8.016475460366144E-5 0.0 0.00553136806765264 0.0 28-29 0.0 8.016475460366144E-5 0.0 0.01114290088990894 0.0 30-31 0.0 8.016475460366144E-5 0.0 0.02044201242393367 0.0 32-33 0.0 8.016475460366144E-5 0.0 0.038118340814041016 0.0 34-35 0.0 8.016475460366144E-5 0.0 0.055714504449544705 0.0 36-37 0.0 8.016475460366144E-5 0.0 0.07655734064649669 0.0 38-39 0.0 8.016475460366144E-5 0.0 0.10637862935905873 0.0 40-41 0.0 8.016475460366144E-5 0.0 0.1420920275349899 0.0 42-43 0.0 8.016475460366144E-5 0.0 0.19227516391688199 0.0 44-45 0.0 8.016475460366144E-5 0.0 0.22794847971551133 0.0 46-47 0.0 8.016475460366144E-5 0.0 0.30222112485580366 0.0 48-49 0.0 8.016475460366144E-5 0.0 0.3799408544440534 0.0 50-51 0.0 8.016475460366144E-5 0.0 0.41052370832535023 0.0 52-53 0.0 8.016475460366144E-5 0.0 0.4643944234190107 0.0 54-55 0.0 8.016475460366144E-5 0.0 0.5751821142812709 0.0 56-57 0.0 8.016475460366144E-5 0.0 0.7419648862341885 0.0 58-59 0.0 8.016475460366144E-5 0.0 0.937446640335217 0.0 60-61 0.0 8.016475460366144E-5 0.0 1.1468770617372823 0.0 62-63 0.0 8.016475460366144E-5 0.0 1.3269671829544079 0.0 64-65 0.0 8.016475460366144E-5 0.0 1.5118671894477531 0.0 66-67 0.0 8.016475460366144E-5 0.0 1.9223908977731032 0.0 68-69 0.0 8.016475460366144E-5 0.0 2.3651408374491254 0.0 70-71 0.0 8.016475460366144E-5 0.0 2.4982544124685053 0.0 72-73 0.0 8.016475460366144E-5 0.0 2.6665202323815906 0.0 74-75 0.0 8.016475460366144E-5 0.0 2.8538652638903477 0.0 76-77 0.0 8.016475460366144E-5 0.0 3.0067394509195298 0.0 78-79 0.0 8.016475460366144E-5 0.0 3.193683658655268 0.0 80-81 0.0 8.016475460366144E-5 0.0 3.548332533021867 0.0 82-83 0.0 8.016475460366144E-5 0.0 4.014731075305969 0.0 84-85 0.0 8.016475460366144E-5 0.0 4.955264058693427 0.0 86-87 0.0 8.016475460366144E-5 0.0 6.28279239493006 0.0 88-89 0.0 8.016475460366144E-5 0.0 7.570719342392485 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1795 0.0 53.464664 2 ACCTGGG 1870 0.0 49.03381 3 TATAACG 210 0.0 47.50944 2 GTACCTG 2125 0.0 46.529484 1 ATAACGC 280 0.0 40.722378 3 GTACCCG 180 0.0 39.61344 1 AGTACGC 620 0.0 36.7815 2 TACCCGG 210 0.0 36.19767 2 TAACGCA 290 0.0 34.390976 4 TACGCAG 705 0.0 33.008835 4 GTACGCA 705 0.0 32.346855 3 GAGTACG 770 0.0 32.10232 1 TACGCGC 60 0.003954216 31.661535 6 CCTGGGG 1420 0.0 29.766304 4 GTACGCG 65 0.0058488906 29.22603 5 ACGCAGT 770 0.0 28.988808 5 GTACACG 185 2.8740033E-10 28.264727 1 CGCAGTG 835 0.0 26.732197 6 CCTGGGA 1160 0.0 24.564983 4 GTATAAC 400 0.0 23.768064 1 >>END_MODULE