##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003796_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2847731 Sequences flagged as poor quality 0 Sequence length 121 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.813361585065444 31.0 31.0 34.0 28.0 34.0 2 30.695324453047004 31.0 31.0 34.0 27.0 34.0 3 30.802819859038653 31.0 31.0 34.0 27.0 34.0 4 34.2250163375684 37.0 35.0 37.0 30.0 37.0 5 34.17730572164295 37.0 35.0 37.0 30.0 37.0 6 34.3174590577551 37.0 35.0 37.0 30.0 37.0 7 34.37363290282685 37.0 35.0 37.0 30.0 37.0 8 34.36469947477483 37.0 35.0 37.0 30.0 37.0 9 35.79233642503453 39.0 35.0 39.0 30.0 39.0 10-11 35.612807881081466 38.5 35.0 39.0 30.0 39.0 12-13 35.469168260625736 38.0 35.0 39.0 30.0 39.0 14-15 36.346786968291596 39.0 36.0 41.0 29.0 41.0 16-17 36.46932803695293 39.0 36.0 41.0 30.0 41.0 18-19 36.49331397523151 39.0 36.0 41.0 30.0 41.0 20-21 36.36796172110357 39.0 36.0 41.0 29.0 41.0 22-23 36.303944965307466 39.0 36.0 41.0 29.0 41.0 24-25 36.02573908841811 39.0 35.5 40.5 27.0 41.0 26-27 35.781372257421786 39.0 35.0 40.0 27.0 41.0 28-29 35.639336369902914 38.0 35.0 40.0 27.0 41.0 30-31 35.4379894027912 38.0 34.5 40.0 26.5 41.0 32-33 35.20538930818957 38.0 34.0 40.0 26.0 41.0 34-35 35.16885460740498 38.0 34.0 40.0 25.0 41.0 36-37 34.98258367802296 38.0 34.0 40.0 25.0 41.0 38-39 34.979368662278844 38.0 34.0 40.0 25.0 41.0 40-41 35.19069146629369 38.0 34.5 40.0 25.0 41.0 42-43 35.066991580314294 38.0 34.0 40.0 24.5 41.0 44-45 34.85598709990515 38.0 34.0 40.0 24.0 41.0 46-47 34.57582001249416 38.0 34.0 40.0 23.5 41.0 48-49 34.258423811799645 38.0 33.0 40.0 23.0 41.0 50-51 33.414331620507696 37.5 32.0 39.5 21.5 40.5 52-53 33.55699379611347 37.5 33.0 39.5 22.0 40.5 54-55 33.91996909118171 38.0 33.0 40.0 22.0 41.0 56-57 33.6191750555091 38.0 33.0 40.0 19.5 41.0 58-59 33.27799992344782 38.0 32.0 40.0 18.0 41.0 60-61 32.84217329516025 37.0 31.0 40.0 14.0 41.0 62-63 32.618904489223176 37.0 31.0 40.0 13.0 41.0 64-65 32.30298455156053 36.0 31.0 39.0 12.5 41.0 66-67 31.828790535341998 36.0 30.5 39.0 8.0 40.5 68-69 31.407961461247567 35.0 30.0 39.0 8.0 40.0 70-71 30.815532436174625 35.0 29.5 37.5 6.5 40.0 72-73 30.374352071877574 35.0 29.0 37.0 3.5 39.0 74-75 30.0158923016254 34.0 29.0 37.0 2.0 39.0 76-77 29.57943411789948 34.0 29.0 36.0 2.0 39.0 78-79 28.98450222299789 34.0 28.5 36.0 2.0 37.5 80-81 28.438944724765086 34.0 26.5 35.0 2.0 37.0 82-83 28.038694666034118 33.5 26.0 35.0 2.0 37.0 84-85 27.770595607520512 33.0 26.0 35.0 2.0 36.0 86-87 27.645054431054056 33.5 26.0 35.0 2.0 36.0 88-89 27.27498260896131 33.0 25.5 35.0 2.0 35.5 90-91 26.830686255127326 33.0 25.0 35.0 2.0 35.0 92-93 26.542404286078987 33.0 24.5 35.0 2.0 35.0 94-95 26.26187234679118 32.5 24.0 35.0 2.0 35.0 96-97 25.92835172985089 32.0 23.0 35.0 2.0 35.0 98-99 25.702591291101584 32.0 20.0 35.0 2.0 35.0 100-101 25.40976289544202 32.0 19.5 35.0 2.0 35.0 102-103 24.922294451266637 32.0 18.5 34.0 2.0 35.0 104-105 24.48526546222238 31.0 16.5 34.0 2.0 35.0 106-107 24.009390985314273 31.0 9.0 34.0 2.0 35.0 108-109 23.733370181383002 31.0 7.0 34.0 2.0 35.0 110-111 23.378940812878746 31.0 2.0 34.0 2.0 35.0 112-113 22.728435024235083 31.0 2.0 34.0 2.0 35.0 114-115 22.103141237708197 30.0 2.0 34.0 2.0 35.0 116-117 21.365995067652108 29.0 2.0 34.0 2.0 35.0 118-119 20.566670447454484 29.0 2.0 34.0 2.0 35.0 120-121 18.894274424094128 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6431994825269776 1101 2 0.5228744356281005 1101 3 0.6237883203268417 1101 4 0.7984251907092812 1101 5 0.8564327485960135 1101 6 0.980452995137405 1101 7 0.7476499010723288 1101 8 0.7929576652344181 1101 9 0.8336640796784209 1101 10-11 0.9549786124086452 1101 12-13 1.0479014420409811 1101 14-15 1.1039249891445166 1101 16-17 0.9543132802557466 1101 18-19 0.8545497228953565 1101 20-21 1.0787785225361048 1101 22-23 1.1078048958925706 1101 24-25 1.0014988097044295 1101 26-27 1.0132462729009433 1101 28-29 1.0502538389182732 1101 30-31 1.0226116757455728 1101 32-33 0.9098528069634 1101 34-35 0.9260061462679872 1101 36-37 0.9513589600213948 1101 38-39 0.9390591542747373 1101 40-41 1.0273932252219211 1101 42-43 0.8433661360860114 1101 44-45 0.9730728624217093 1101 46-47 0.9230261298277185 1101 48-49 1.0698254592988903 1101 50-51 0.7742182564968729 1101 52-53 0.76135427674906 1101 54-55 0.8809645906462436 1101 56-57 0.8233285002411677 1101 58-59 0.8620800897213385 1101 60-61 0.8706065465921498 1101 62-63 0.8134832545712456 1101 64-65 0.8242334374590783 1101 66-67 0.8372232367486099 1101 68-69 0.7661437887452855 1101 70-71 0.46588756643733475 1101 72-73 0.28626564275781874 1101 74-75 0.3352244880215238 1101 76-77 0.3937228598070277 1101 78-79 0.6452216817950891 1101 80-81 0.6666996338545808 1101 82-83 0.639759285590948 1101 84-85 0.7860230817530613 1101 86-87 0.6957429514927291 1101 88-89 0.8454310027636147 1101 90-91 0.7440273059313753 1101 92-93 0.7177383539436875 1101 94-95 0.7773166871284758 1101 96-97 0.6902287571030605 1101 98-99 0.5728500143760726 1101 100-101 0.4233243513315408 1101 102-103 0.34653828090567984 1101 104-105 0.31306996765070494 1101 106-107 0.1331803823868256 1101 108-109 0.19656074189154538 1101 110-111 0.2057400490056267 1101 112-113 0.39309791970507746 1101 114-115 0.14224831794408743 1101 116-117 -0.09898076534242506 1101 118-119 0.09648480983463159 1101 120-121 -0.06313203389164457 1102 1 0.34943690848724884 1102 2 0.5149181093960173 1102 3 0.5549496773335356 1102 4 0.8340725238395024 1102 5 0.8687937414127518 1102 6 0.8610512189213395 1102 7 0.6209812969594282 1102 8 0.7160848626372882 1102 9 0.7416123650794688 1102 10-11 0.738901000013449 1102 12-13 0.7589825616196038 1102 14-15 0.958756504473655 1102 16-17 0.8019041267235139 1102 18-19 0.7831278312588381 1102 20-21 0.9649807490855196 1102 22-23 0.714388744730563 1102 24-25 0.9280342894909879 1102 26-27 0.9721981230704131 1102 28-29 0.9468645525589992 1102 30-31 0.9042947094633362 1102 32-33 0.75550571951743 1102 34-35 0.8528079566371005 1102 36-37 0.9908094189632379 1102 38-39 0.7965973397365715 1102 40-41 0.8807849987378162 1102 42-43 0.94078027739139 1102 44-45 0.8439393983205079 1102 46-47 0.8275108887075362 1102 48-49 0.7938125223391239 1102 50-51 0.44024075935359264 1102 52-53 0.5205329673061598 1102 54-55 0.6407059638118469 1102 56-57 0.6129465350175778 1102 58-59 0.5786850963617454 1102 60-61 0.7350514196728426 1102 62-63 0.5964479405756293 1102 64-65 0.6407433118219714 1102 66-67 0.553134238911209 1102 68-69 0.4127555850306557 1102 70-71 0.3035203645072535 1102 72-73 0.3007188317198093 1102 74-75 0.44812864073036707 1102 76-77 0.33476835276907835 1102 78-79 0.57028698867569 1102 80-81 0.7285614221517882 1102 82-83 0.7197245309422158 1102 84-85 0.6147254074961381 1102 86-87 0.6496755440474296 1102 88-89 0.7212955530336131 1102 90-91 0.7911443519898889 1102 92-93 0.8380235096683641 1102 94-95 0.6717073355132328 1102 96-97 0.7712089964389257 1102 98-99 0.7571551080845289 1102 100-101 0.8944898961247638 1102 102-103 0.8169239704268385 1102 104-105 0.8388616061929923 1102 106-107 0.6092413536560102 1102 108-109 0.5336886179077815 1102 110-111 0.46454256443974984 1102 112-113 0.5175767726120597 1102 114-115 0.4857939358491805 1102 116-117 0.5492321160688967 1102 118-119 0.40681483152380693 1102 120-121 0.43363735448903995 1103 1 0.6248737285502166 1103 2 0.7004528511337895 1103 3 0.7092477737784009 1103 4 0.9399515607266338 1103 5 0.9709002997736675 1103 6 1.0030221179783183 1103 7 0.7832151544316872 1103 8 0.8209694335982789 1103 9 0.8205730997302254 1103 10-11 0.9160411042309633 1103 12-13 0.9112819765462348 1103 14-15 1.138170616105107 1103 16-17 0.9535624573284949 1103 18-19 0.9269572968743915 1103 20-21 1.046995697950571 1103 22-23 1.0506425797937737 1103 24-25 1.1265203823806687 1103 26-27 1.1733976005350257 1103 28-29 1.0932089649378653 1103 30-31 1.1285352747057686 1103 32-33 1.0641055904248518 1103 34-35 1.051739089667386 1103 36-37 1.0873984253566888 1103 38-39 0.9797170833822335 1103 40-41 1.1376044522054016 1103 42-43 1.0788107934954994 1103 44-45 1.1028573068338474 1103 46-47 1.0498401691378234 1103 48-49 1.0847566823242545 1103 50-51 0.8179499988609606 1103 52-53 0.8235675887785945 1103 54-55 0.8968668185878315 1103 56-57 0.9028867129355618 1103 58-59 0.9675022412018492 1103 60-61 0.8479268051641284 1103 62-63 0.9014228623330922 1103 64-65 0.9042010034093337 1103 66-67 0.8254050446983321 1103 68-69 0.7816898916647723 1103 70-71 0.7143766584011821 1103 72-73 0.539994374326664 1103 74-75 0.5589441027898054 1103 76-77 0.6067374443430786 1103 78-79 0.7015832295314368 1103 80-81 0.8000458560803452 1103 82-83 0.7865908925630478 1103 84-85 0.8685266095890789 1103 86-87 0.8778131733677021 1103 88-89 0.8894589004547271 1103 90-91 0.7838996496197517 1103 92-93 0.8358330457991094 1103 94-95 0.9684843645062884 1103 96-97 0.9063379068723059 1103 98-99 0.815776580048226 1103 100-101 0.7750202919635392 1103 102-103 0.8300459699233578 1103 104-105 0.950946334643195 1103 106-107 1.0426274324874854 1103 108-109 0.9635646418562303 1103 110-111 1.2753714892115617 1103 112-113 1.2810422289034307 1103 114-115 1.2371729589583396 1103 116-117 1.1956444258219712 1103 118-119 0.9804678363591712 1103 120-121 0.9042574659364782 1104 1 0.5271281491647528 1104 2 0.6819046487583229 1104 3 0.6814493489123237 1104 4 0.8772956701802457 1104 5 0.982027319956714 1104 6 0.8023860619152146 1104 7 0.681101923686974 1104 8 0.6763448791453399 1104 9 0.8031642846658684 1104 10-11 0.9581887021924658 1104 12-13 1.0339604239737241 1104 14-15 1.0842404088968607 1104 16-17 0.9675723492202408 1104 18-19 0.9590487946096502 1104 20-21 0.9579993410928296 1104 22-23 0.8585861056565491 1104 24-25 0.9822884118155173 1104 26-27 1.077355217318832 1104 28-29 0.9198805190042165 1104 30-31 0.9826742337846994 1104 32-33 0.9631377449578196 1104 34-35 0.926054755631796 1104 36-37 0.9161805846427598 1104 38-39 0.7556563649480594 1104 40-41 0.8029936274374663 1104 42-43 0.8222220837961771 1104 44-45 0.9222276380392955 1104 46-47 1.0441590762675688 1104 48-49 1.168893441394303 1104 50-51 0.8031971512980434 1104 52-53 0.924218560539714 1104 54-55 1.0042179716912472 1104 56-57 1.042363314528437 1104 58-59 1.1401461267615574 1104 60-61 1.0444207560450778 1104 62-63 1.1667364631862966 1104 64-65 1.0258805064789343 1104 66-67 0.9360498736363247 1104 68-69 1.0589472546780065 1104 70-71 0.7573275312071708 1104 72-73 0.671124069829137 1104 74-75 0.739209366822557 1104 76-77 0.847336254165441 1104 78-79 0.9137582415952714 1104 80-81 0.884457842597822 1104 82-83 0.8576726812879549 1104 84-85 1.0233178305577901 1104 86-87 0.9336979254955118 1104 88-89 0.867291887188582 1104 90-91 0.8865822345124776 1104 92-93 0.9188082363986432 1104 94-95 0.8255014421883295 1104 96-97 0.7645329625560677 1104 98-99 0.7468272381949106 1104 100-101 0.7973759582538769 1104 102-103 0.7999291612218222 1104 104-105 0.6524999664232709 1104 106-107 0.7155257223653635 1104 108-109 0.8432487142446732 1104 110-111 0.8564796146681992 1104 112-113 0.7103505343443324 1104 114-115 0.7600162712482401 1104 116-117 0.5505099310408781 1104 118-119 0.4614734079042755 1104 120-121 0.5780669940572061 1105 1 0.7369409341040303 1105 2 0.8651050759223722 1105 3 0.8928514478061516 1105 4 1.0273197990370235 1105 5 1.1738227579253788 1105 6 1.0495144698962946 1105 7 1.0826724929098148 1105 8 1.1184746841837097 1105 9 1.2327976409980579 1105 10-11 1.2288639770467782 1105 12-13 1.419204953573626 1105 14-15 1.5483021365417784 1105 16-17 1.4205491134948005 1105 18-19 1.1762646342129273 1105 20-21 1.2810151150543518 1105 22-23 1.272826963963503 1105 24-25 1.3758060190774941 1105 26-27 1.556968444765154 1105 28-29 1.5224685705632552 1105 30-31 1.43891906813311 1105 32-33 1.4852377601835158 1105 34-35 1.4554921368496707 1105 36-37 1.6022263283570055 1105 38-39 1.3774374734021748 1105 40-41 1.4179724393217654 1105 42-43 1.5382941194013213 1105 44-45 1.471426400464722 1105 46-47 1.5299432265763855 1105 48-49 1.5170197658714812 1105 50-51 1.3267743405313865 1105 52-53 1.3830929945320989 1105 54-55 1.489876375114001 1105 56-57 1.5655015626725586 1105 58-59 1.631137687878649 1105 60-61 1.6984433895461635 1105 62-63 1.6087939520350574 1105 64-65 1.7189660230417516 1105 66-67 1.6733711975164276 1105 68-69 1.6208380069094837 1105 70-71 1.4037068771873003 1105 72-73 1.3556853082107487 1105 74-75 1.4302014088745665 1105 76-77 1.5624297045121907 1105 78-79 1.737695885685131 1105 80-81 1.6677469846591926 1105 82-83 1.7860741990716384 1105 84-85 1.6920305625003884 1105 86-87 1.7621915481811854 1105 88-89 1.7420073438034436 1105 90-91 1.7080306298437975 1105 92-93 1.921381824719763 1105 94-95 1.9457457289747495 1105 96-97 1.7671415652852893 1105 98-99 1.8306887397300322 1105 100-101 1.8803073199260858 1105 102-103 1.7912112774105395 1105 104-105 1.8037294237574244 1105 106-107 1.8438997447478407 1105 108-109 1.9932355413199474 1105 110-111 1.9560907963815986 1105 112-113 1.7134493974376426 1105 114-115 1.606282022746587 1105 116-117 1.5999117418429591 1105 118-119 1.4817549026312342 1105 120-121 1.3857157830044322 1106 1 0.5474827688793091 1106 2 0.7007735793100132 1106 3 0.7221295901584988 1106 4 0.9732555684995248 1106 5 0.9508713448109631 1106 6 0.8135012525198064 1106 7 0.8081055461212756 1106 8 0.7919552558177045 1106 9 0.904597356158078 1106 10-11 1.0350005624492908 1106 12-13 1.0640726983900777 1106 14-15 1.0054052238112803 1106 16-17 0.9769533783283322 1106 18-19 0.9287234770912391 1106 20-21 1.0464549072238896 1106 22-23 0.9213792153141185 1106 24-25 1.1777487930480746 1106 26-27 1.1994362812715096 1106 28-29 1.1837334654768483 1106 30-31 0.9851966079875396 1106 32-33 0.9053824849446812 1106 34-35 0.9751251767836351 1106 36-37 1.011388868768833 1106 38-39 1.1109981108725364 1106 40-41 1.0932986462278294 1106 42-43 0.9510998307309038 1106 44-45 0.9070150509570496 1106 46-47 1.025248753148297 1106 48-49 1.0689257135656405 1106 50-51 0.8668315590452309 1106 52-53 0.9903386270484518 1106 54-55 1.0486154759766677 1106 56-57 0.8255417960058438 1106 58-59 1.0445318680743796 1106 60-61 1.1172220974449019 1106 62-63 1.0691985024601252 1106 64-65 0.9162683104044405 1106 66-67 1.1408277919877285 1106 68-69 1.002905927590195 1106 70-71 0.8211554550542033 1106 72-73 0.9709680876676963 1106 74-75 0.8340591492192573 1106 76-77 1.0259361797991318 1106 78-79 1.0156304952451833 1106 80-81 0.8725209978339805 1106 82-83 1.1208698946561206 1106 84-85 0.9647506214224286 1106 86-87 0.8644718963523346 1106 88-89 0.8883805818727986 1106 90-91 1.0045247432762459 1106 92-93 0.867063317511704 1106 94-95 0.7387622644738983 1106 96-97 0.9748539771183005 1106 98-99 0.9899826146684987 1106 100-101 0.805190734698936 1106 102-103 0.8076662973706341 1106 104-105 0.96061700215418 1106 106-107 1.009055385869516 1106 108-109 1.0937799188619302 1106 110-111 0.8836601494040472 1106 112-113 0.7726599122163798 1106 114-115 0.7758076527076447 1106 116-117 0.8495748565693368 1106 118-119 0.8974351303027994 1106 120-121 0.7975050139490243 1107 1 0.49942020492590444 1107 2 0.6589174926491381 1107 3 0.5246116045941172 1107 4 0.7803054118669053 1107 5 0.7685209777626412 1107 6 0.7356166509929167 1107 7 0.7173296414713022 1107 8 0.7766215058455472 1107 9 0.9708623201083384 1107 10-11 0.8187807343717637 1107 12-13 0.8501986910189743 1107 14-15 0.9261863954048977 1107 16-17 0.7293537682393918 1107 18-19 0.6851898950847897 1107 20-21 0.7388640884761841 1107 22-23 0.7020299448906258 1107 24-25 0.6453953470650404 1107 26-27 0.8084077729564214 1107 28-29 0.9778891233141778 1107 30-31 0.8731581697804458 1107 32-33 0.8023931668828581 1107 34-35 0.8526978232655864 1107 36-37 0.8336637822638693 1107 38-39 0.8166250933461825 1107 40-41 0.9375220406288847 1107 42-43 1.0013279339367998 1107 44-45 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-1.4462264045676712 2316 68-69 -1.612165704271579 2316 70-71 -1.3868068282682628 2316 72-73 -1.5516528195344144 2316 74-75 -1.486403070913493 2316 76-77 -1.5121841536403622 2316 78-79 -1.518120901247535 2316 80-81 -1.5360863261068332 2316 82-83 -1.530719453074358 2316 84-85 -1.4161574906205203 2316 86-87 -1.4569840452502554 2316 88-89 -1.4029976869312009 2316 90-91 -1.2218675789205342 2316 92-93 -1.4176527331816118 2316 94-95 -1.3556737546751094 2316 96-97 -1.255754362913141 2316 98-99 -1.1064055875588146 2316 100-101 -0.982711341462366 2316 102-103 -0.9018499474414945 2316 104-105 -1.3199113396971285 2316 106-107 -1.336725815948327 2316 108-109 -1.267216481851861 2316 110-111 -1.4433700097834858 2316 112-113 -1.5795542554234103 2316 114-115 -1.4882553449113658 2316 116-117 -1.6052688879015626 2316 118-119 -1.712744227386377 2316 120-121 -1.9734516025197308 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39222.0 3 6025.0 4 5285.0 5 5987.0 6 7116.0 7 7596.0 8 9428.0 9 11472.0 10 14212.0 11 17011.0 12 19377.0 13 22868.0 14 27082.0 15 28388.0 16 24781.0 17 25545.0 18 27165.0 19 29634.0 20 32122.0 21 35231.0 22 39126.0 23 42887.0 24 47851.0 25 53055.0 26 60755.0 27 69593.0 28 80229.0 29 93371.0 30 109117.0 31 128942.0 32 155496.0 33 188998.0 34 235677.0 35 294848.0 36 369357.0 37 367641.0 38 114733.0 39 508.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.57090555252333 17.72722605983907 13.753537383452896 20.948331004184706 2 13.172091130568187 22.32236695887725 37.111641787235584 27.393900123318986 3 25.07126132365649 23.614524620867073 30.730697060155826 20.583516995320608 4 10.76820202653044 14.505135781224862 44.08869561149922 30.63796658074548 5 11.005582513605427 40.15146360237986 35.888807613085596 12.95414627092911 6 26.40116451958074 36.97852961326934 18.016098979911185 18.604206887238735 7 24.680134536965774 33.96657978175304 22.92659713038832 18.426688550892873 8 21.58456702465682 29.263002298733287 20.679812813383105 28.472617863226784 9 20.924553175855287 21.323237902435793 26.680982423222275 31.07122649848664 10-11 24.601385585405584 23.063769095550555 28.151788769786357 24.183056549257504 12-13 30.066255454419526 21.77521892960349 26.390640061982968 21.767885553994017 14-15 24.03220458113922 24.137298845362462 30.758928318378402 21.071568255119914 16-17 19.60544318452021 28.020862162383402 30.84155252872924 21.532142124367148 18-19 18.10299357557385 32.72738938034276 29.00812375846653 20.161493285616856 20-21 20.790848131489966 27.561725862542268 25.816730469419426 25.830695536548337 22-23 18.966832908736595 27.21024616689159 27.620793032963697 26.20212789140812 24-25 23.62783003190994 26.462166913863 26.988383307621604 22.92161974660545 26-27 22.803844482392506 23.363463244325462 32.541508571886155 21.291183701395873 28-29 19.01729636090294 29.778064766727365 30.241023463709325 20.96361540866037 30-31 20.008166077603256 27.212992853264375 28.28349686754367 24.495344201588697 32-33 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28.038166632831 24.24590049122297 58-59 25.241222410962166 24.957214481993926 27.256608182491043 22.54495492455286 60-61 22.45102535090529 25.508314784243524 31.938740763049612 20.101919101801577 62-63 21.287574731236912 26.746942345834263 30.314973467486862 21.650509455441966 64-65 19.510197152990607 30.847823066956604 28.51852101235821 21.12345876769458 66-67 20.532343579419795 27.000758227763537 26.795581334236328 25.671316858580333 68-69 18.94088484859656 28.455573601858543 28.05079014213413 24.55275140741077 70-71 22.80465038120959 27.166882093394758 26.320283413181844 23.70818411221381 72-73 21.580021509504935 24.6156022439799 30.667955747925053 23.136420498590113 74-75 19.46189250223674 30.297876504446265 28.450004422951913 21.790226570365085 76-77 20.724219187282113 26.29595293449348 27.895351123493413 25.08447675473099 78-79 21.304461107576643 26.441892336979166 29.359083483190968 22.894563072253224 80-81 23.414401001453356 26.256447139840134 27.654220410573167 22.674931448133346 82-83 21.616715043943927 24.88515200089672 31.26320206614663 22.234930889012723 84-85 21.272737297509277 26.80358198032219 29.801011139482963 22.12266958268557 86-87 21.104482088344245 28.424457087998594 28.55403205021624 21.917028773440926 88-89 20.295969071201927 27.360805076145912 26.779410036473678 25.563815816178487 90-91 20.570204253698773 24.73813696545567 28.19641668761476 26.495242093230793 92-93 22.358949652565443 27.17554724354877 28.431405914381603 22.034097189504184 94-95 21.220515516414146 26.05692704687865 28.949291500361664 23.773265936345545 96-97 17.227232763266116 26.881469979296067 32.03542003460109 23.85587722283672 98-99 19.750007181459704 28.692538126458235 27.713696302629902 23.843758389452155 100-101 18.65185779067877 25.90909115086093 29.54983501533007 25.889216043130226 102-103 20.73833090675892 24.406605954448466 29.328794274347903 25.526268864444713 104-105 23.201012631844982 24.9253898258644 28.451328680524895 23.422268861765723 106-107 21.08672947000577 26.041792761032838 30.85198525628608 22.01949251267531 108-109 19.722576143483707 26.078679044744018 30.328581700397052 23.870163111375227 110-111 18.775184104487984 28.781479244469775 28.36710236408027 24.07623428696197 112-113 21.225351438634053 25.462076886672897 26.014736405515247 27.2978352691778 114-115 19.453619831101197 25.507058897976336 28.334358160260482 26.70496311066199 116-117 22.11013133534253 24.941339338936814 27.237394030495388 25.71113529522527 118-119 21.100935160702047 24.08133660643716 30.12663833963854 24.691089893222255 120-121 18.966774833438123 28.767333079622663 27.25132617590374 25.014565911035476 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6883.0 1 7727.5 2 4949.0 3 1355.0 4 1442.5 5 1452.5 6 1432.0 7 1577.5 8 1704.0 9 1655.0 10 1565.0 11 1521.5 12 1580.0 13 1756.5 14 1950.0 15 2133.0 16 2352.0 17 2563.0 18 2821.5 19 3137.5 20 3468.0 21 3968.0 22 4675.5 23 5776.5 24 7182.0 25 8796.5 26 11013.0 27 13898.5 28 17746.0 29 22288.0 30 27002.0 31 31638.5 32 37029.0 33 42455.0 34 46957.5 35 52520.5 36 57911.5 37 63784.5 38 68850.5 39 71103.5 40 73784.0 41 76210.0 42 79022.0 43 84937.5 44 90629.0 45 97090.5 46 115428.5 47 193744.0 48 370322.0 49 367762.0 50 194421.0 51 111106.0 52 75169.5 53 58143.5 54 48042.5 55 39522.5 56 31410.5 57 23838.0 58 18259.5 59 14698.0 60 11572.0 61 8636.5 62 6246.5 63 4244.5 64 2740.0 65 1728.0 66 1064.0 67 640.5 68 366.0 69 232.5 70 143.5 71 85.0 72 58.0 73 43.0 74 35.5 75 27.5 76 20.0 77 15.0 78 9.0 79 10.0 80 13.5 81 10.5 82 8.0 83 8.0 84 7.0 85 4.5 86 5.5 87 4.0 88 2.0 89 4.0 90 3.5 91 2.0 92 3.5 93 4.5 94 3.5 95 2.0 96 1.0 97 1.5 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46921566678875215 2 0.933796064305231 3 0.9279317463622794 4 0.904755400000913 5 0.915219871539833 6 0.9238934435871928 7 0.8992071231447071 8 0.8886724202531769 9 0.8849150428885313 10-11 0.8762414708411714 12-13 0.8785942211536132 14-15 0.878840030887749 16-17 0.9052821351454896 18-19 0.9348846502706892 20-21 0.9272469906743299 22-23 0.9004186139772332 24-25 0.8923946819415176 26-27 0.8937817511555691 28-29 0.9164138045342064 30-31 0.923647633853057 32-33 0.9174672748233593 34-35 0.9289676588132798 36-37 0.9382733130341313 38-39 0.9250873765815663 40-41 0.9331113086172815 42-43 0.9266675820152956 44-45 0.9090921860245929 46-47 0.9034210043013191 48-49 0.9069501297699819 50-51 0.9498263705385094 52-53 0.911585399042255 54-55 0.923647633853057 56-57 0.9737050304259778 58-59 0.9369915908489953 60-61 0.9503179900067809 62-63 0.9230857830321755 64-65 0.9117083039093229 66-67 0.913692339620561 68-69 0.9338311799815362 70-71 0.9471751369774745 72-73 0.9522493522035614 74-75 0.9557258041577662 76-77 0.9683674476276025 78-79 1.0066435348001619 80-81 1.033349006630191 82-83 1.0032548720367196 84-85 0.9638375253842445 86-87 0.9470697899485591 88-89 0.9456476050582024 90-91 0.9575342614874791 92-93 0.9450330807228633 94-95 0.938800048178708 96-97 0.9485095326770683 98-99 0.9821854662536594 100-101 0.9699125373850269 102-103 0.9439796104337101 104-105 0.932935730235756 106-107 0.9339540848486041 108-109 0.9326899205016204 110-111 0.9308112318192976 112-113 0.9420131325606245 114-115 0.9456476050582024 116-117 0.9726340022986721 118-119 0.9682796584368398 120-121 0.9457529520871177 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 2847731.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.48467270771521 #Duplication Level Percentage of deduplicated Percentage of total 1 78.82441690952597 31.12356303049125 2 12.442144610068462 9.825480154212322 3 3.5176240270775825 4.166767002538604 4 1.5130216781962746 2.3896466305303163 5 0.8265861504537665 1.6318741807698607 6 0.49922237454360346 1.182697924033356 7 0.3545622970715515 0.9799843378056132 8 0.25089199973884874 0.7925110795738091 9 0.20009678310247345 0.711068039160106 >10 1.3234266781581931 10.216036024971668 >50 0.14615036523398361 4.018673958730674 >100 0.08867792967197904 6.512541363501045 >500 0.004699916468753456 1.2554671795119572 >1k 0.005529313492651123 4.592936534988761 >5k 0.001290173148285262 3.4421189108278285 >10k+ 0.001658794047795337 17.158633648352893 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 83129 2.919131055566695 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 73725 2.5889032355935306 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 46526 1.6337919557711034 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 41900 1.4713468371837088 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 27306 0.9588686571870728 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 21342 0.7494387637034537 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 19668 0.6906551215687156 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19527 0.6857038112096964 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 18735 0.6578921955760568 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 17317 0.6080981665754245 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 15358 0.5393065566937327 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 15340 0.5386744745202409 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 14400 0.5056657387934465 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11824 0.4152077566315077 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 11725 0.41173130467730273 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 11258 0.3953322838428208 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 11242 0.39477043302193926 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 11235 0.39452462328780347 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 9667 0.339463242841406 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8765 0.3077889028142054 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 8094 0.284226284013483 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8010 0.2812765672038546 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7122 0.25009384664492534 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6651 0.23355436310522307 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 6231 0.21880577905708087 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6092 0.2139247000506719 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 5970 0.20964058754144965 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5856 0.20563740044266823 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 5784 0.20310907174870096 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5583 0.19605082081137581 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5399 0.1895895363712373 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5373 0.18867652878730468 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4939 0.17343632527089112 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 4253 0.14934697132559221 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 4081 0.14330707500111492 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3982 0.13983062304690996 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3971 0.13944435060755386 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3953 0.13881226843406208 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3870 0.1358976673007387 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3641 0.12785617742687072 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3536 0.12416903141483518 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3247 0.11402060096266114 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2987 0.10489052512333502 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2977 0.10453936836028403 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2956 0.10380193915787693 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.511567630510045E-5 0.0 14-15 0.0 0.0 0.0 1.7557838152550224E-4 0.0 16-17 0.0 0.0 0.0 3.335989248984542E-4 0.0 18-19 0.0 0.0 0.0 5.618508208816071E-4 0.0 20-21 0.0 0.0 0.0 9.305654220851618E-4 0.0 22-23 0.0 0.0 0.0 0.0016504367863397211 0.0 24-25 0.0 3.511567630510045E-5 0.0 0.002545886532119782 0.0 26-27 0.0 7.02313526102009E-5 0.0 0.0033359892489845423 0.0 28-29 0.0 7.02313526102009E-5 0.0 0.006724652012426736 0.0 30-31 0.0 7.02313526102009E-5 0.0 0.015661591632074798 0.0 32-33 3.511567630510045E-5 7.02313526102009E-5 0.0 0.03741575310308452 0.0 34-35 3.511567630510045E-5 7.02313526102009E-5 0.0 0.06096081406565437 0.0 36-37 3.511567630510045E-5 7.02313526102009E-5 0.0 0.07651705866881386 0.0 38-39 3.511567630510045E-5 7.02313526102009E-5 0.0 0.09207330327197338 0.0 40-41 3.511567630510045E-5 7.02313526102009E-5 0.0 0.11509162908996672 0.0 42-43 3.511567630510045E-5 7.02313526102009E-5 0.0 0.142639877151318 0.0 44-45 3.511567630510045E-5 7.02313526102009E-5 0.0 0.16785293273838015 0.0 46-47 3.511567630510045E-5 7.02313526102009E-5 0.0 0.19824555058044457 0.0 48-49 3.511567630510045E-5 7.02313526102009E-5 0.0 0.2200699434040645 0.0 50-51 3.511567630510045E-5 7.02313526102009E-5 0.0 0.2549222521368767 0.0 52-53 3.511567630510045E-5 7.02313526102009E-5 0.0 0.38567547285891823 0.0 54-55 3.511567630510045E-5 7.02313526102009E-5 0.0 0.5379897188322913 0.0 56-57 3.511567630510045E-5 7.02313526102009E-5 0.0 0.6595075166860915 0.0 58-59 3.511567630510045E-5 7.02313526102009E-5 0.0 0.8705351734415927 0.0 60-61 3.511567630510045E-5 7.02313526102009E-5 0.0 1.106178919286969 0.0 62-63 3.511567630510045E-5 7.02313526102009E-5 0.0 1.1866640493782592 0.0 64-65 3.511567630510045E-5 7.02313526102009E-5 0.0 1.2508730635021355 0.0 66-67 3.511567630510045E-5 7.02313526102009E-5 0.0 1.6314392054586615 0.0 68-69 3.511567630510045E-5 7.02313526102009E-5 0.0 1.9323454357170675 0.0 70-71 3.511567630510045E-5 7.02313526102009E-5 0.0 1.9995743980031824 0.0 72-73 3.511567630510045E-5 7.02313526102009E-5 0.0 2.126219084597527 0.0 74-75 3.511567630510045E-5 7.02313526102009E-5 0.0 2.3249035811317853 0.0 76-77 3.511567630510045E-5 7.02313526102009E-5 0.0 2.4820286747589573 0.0 78-79 3.511567630510045E-5 7.02313526102009E-5 0.0 2.6375384472760945 0.0 80-81 3.511567630510045E-5 7.02313526102009E-5 0.0 2.925100720538562 0.0 82-83 3.511567630510045E-5 7.02313526102009E-5 0.0 3.2495871274358428 0.0 84-85 3.511567630510045E-5 7.02313526102009E-5 0.0 3.620636921113687 1.7557838152550224E-5 86-87 3.511567630510045E-5 7.02313526102009E-5 0.0 4.365124374458121 3.511567630510045E-5 88-89 3.511567630510045E-5 7.02313526102009E-5 0.0 5.295356197618385 3.511567630510045E-5 90-91 3.511567630510045E-5 7.02313526102009E-5 0.0 6.339538390388698 3.511567630510045E-5 92-93 3.511567630510045E-5 7.02313526102009E-5 0.0 7.406545772757329 3.511567630510045E-5 94-95 3.511567630510045E-5 7.02313526102009E-5 0.0 8.2273571485509 3.511567630510045E-5 96-97 3.511567630510045E-5 1.0534702891530134E-4 0.0 9.090114199690912 3.511567630510045E-5 98-99 3.511567630510045E-5 1.404627052204018E-4 0.0 10.039010004807336 3.511567630510045E-5 100-101 3.511567630510045E-5 1.404627052204018E-4 0.0 10.869179708336215 3.511567630510045E-5 102-103 3.511567630510045E-5 1.404627052204018E-4 0.0 11.485284249109203 3.511567630510045E-5 104-105 3.511567630510045E-5 1.7557838152550224E-4 0.0 12.187562659534906 3.511567630510045E-5 106-107 3.511567630510045E-5 2.1069405783060268E-4 0.0 13.045982924651241 3.511567630510045E-5 108-109 3.511567630510045E-5 2.282518959831529E-4 0.0 14.001778960161616 3.511567630510045E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2060 0.0 32.674095 2 TATAACG 385 0.0 29.885065 2 GTACCCG 290 0.0 29.813957 1 GGCGTCG 515 0.0 28.98856 8 ACCTGGG 2225 0.0 28.916187 3 CGGGCGT 500 0.0 28.724588 6 GTACCGG 230 3.8562575E-10 27.567108 1 GTATAAC 690 0.0 26.73174 1 GTACTAG 605 0.0 26.676512 1 GAGTACG 1910 0.0 24.444323 1 ACGGGCG 610 0.0 23.544329 5 AGTACGC 1725 0.0 23.0115 2 GTATAGG 1050 0.0 21.958216 1 ATAACGC 555 0.0 21.735985 3 GTACGCA 1720 0.0 21.708904 3 GAGTACT 6340 0.0 21.25215 12-13 GCGTCGG 690 0.0 20.80422 9 TAACGCA 580 0.0 20.79872 4 GGGCGTC 720 0.0 20.738548 7 AGTACCG 365 1.8189894E-10 20.460989 5 >>END_MODULE