##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003796_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2847731 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.630631193746883 31.0 31.0 34.0 30.0 34.0 2 31.825310747398543 31.0 31.0 34.0 30.0 34.0 3 31.88456037455785 31.0 31.0 34.0 30.0 34.0 4 35.55084310983025 37.0 35.0 37.0 33.0 37.0 5 35.46673369078751 37.0 35.0 37.0 33.0 37.0 6 35.54194374398425 37.0 35.0 37.0 33.0 37.0 7 35.488451683111926 37.0 35.0 37.0 33.0 37.0 8 35.507353749353435 37.0 35.0 37.0 33.0 37.0 9 37.04240112566812 39.0 37.0 39.0 33.0 39.0 10-11 36.94073966255942 39.0 37.0 39.0 33.0 39.0 12-13 36.91416517220201 39.0 37.0 39.0 33.0 39.0 14-15 37.83916370612252 39.0 37.0 41.0 32.5 41.0 16-17 37.93017054630511 39.5 37.0 41.0 33.0 41.0 18-19 38.02279393664641 40.0 37.0 41.0 33.0 41.0 20-21 37.95576091983407 40.0 37.0 41.0 32.5 41.0 22-23 37.85837566820743 39.5 37.0 41.0 32.5 41.0 24-25 37.68943274487654 39.5 36.5 41.0 32.0 41.0 26-27 37.344810131294004 39.0 36.0 40.5 31.5 41.0 28-29 37.22920879816246 39.0 36.0 40.0 31.0 41.0 30-31 37.11094025383718 39.0 36.0 40.0 31.0 41.0 32-33 36.888832898893895 39.0 35.5 40.0 30.5 41.0 34-35 36.84465930946428 39.0 35.0 40.0 30.0 41.0 36-37 36.617517068852365 38.5 35.0 40.0 30.0 41.0 38-39 36.34652061588682 38.0 35.0 40.0 30.0 41.0 40-41 36.65959179430922 39.0 35.0 40.0 30.0 41.0 42-43 36.57893846012843 39.0 35.0 40.0 30.0 41.0 44-45 36.449039603810895 39.0 35.0 40.0 30.0 41.0 46-47 36.20557682590105 39.0 35.0 40.0 30.0 41.0 48-49 35.91131448159956 38.5 35.0 40.0 28.0 41.0 50-51 35.58601813162831 38.0 34.0 40.0 28.0 41.0 52-53 35.44319319486286 38.0 34.0 40.0 28.0 41.0 54-55 35.27413755021102 38.0 34.0 40.0 27.0 41.0 56-57 34.95805327118327 38.0 34.0 40.0 26.5 41.0 58-59 34.68649303603465 38.0 34.0 40.0 26.0 41.0 60-61 34.03158795546349 37.0 32.5 40.0 23.5 41.0 62-63 33.886001521913414 37.0 33.0 39.5 25.0 41.0 64-65 33.428649861942716 36.0 32.0 39.0 23.0 40.5 66-67 33.01115730383242 36.0 32.0 39.0 22.0 40.0 68-69 32.65012443239898 35.5 31.0 38.5 22.0 40.0 70-71 32.08355582040579 35.0 31.0 37.5 21.5 39.5 72-73 31.603718715005034 35.0 30.5 37.0 20.5 39.0 74-75 31.259863378949767 34.0 30.0 36.5 20.0 39.0 76-77 30.310641173622088 33.5 29.5 35.0 20.0 38.0 78-79 30.688880199709875 34.0 30.0 36.0 19.5 37.5 80-81 30.439634221069333 34.0 30.0 35.0 18.0 37.0 82-83 30.113295813403724 34.0 29.5 35.0 14.5 37.0 84-85 29.77687130561138 34.0 29.0 35.0 11.5 36.0 86-87 29.581849549694127 34.0 29.0 35.0 8.5 36.0 88-89 29.2393272047114 34.0 29.0 35.0 7.0 36.0 90-91 28.853197159422713 34.0 29.0 35.0 2.0 35.0 92-93 28.651154199606637 34.0 29.0 35.0 2.0 35.0 94-95 28.251404714841396 34.0 29.0 35.0 2.0 35.0 96-97 27.74136426509386 33.5 27.0 35.0 2.0 35.0 98-99 27.43782225217199 34.0 27.0 35.0 2.0 35.0 100-101 26.34182512322969 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05572032557340023 1101 2 0.05503242632224925 1101 3 0.13606826411130157 1101 4 0.10748405882140588 1101 5 0.15569298683931265 1101 6 0.1368713222100908 1101 7 0.14614010722051773 1101 8 0.08434865817280723 1101 9 0.1479064926122433 1101 10-11 0.2612569305125021 1101 12-13 0.2007606859472162 1101 14-15 0.217450363302504 1101 16-17 0.1973457919030679 1101 18-19 0.06961281881456927 1101 20-21 0.05764148798320434 1101 22-23 0.16234764069314167 1101 24-25 0.14101173146260493 1101 26-27 0.3184717179439147 1101 28-29 0.15120201343868445 1101 30-31 0.27779871797695677 1101 32-33 0.32602732127878653 1101 34-35 0.32996789206891464 1101 36-37 0.3615522522662076 1101 38-39 0.22080124879568075 1101 40-41 0.11018834779737574 1101 42-43 -0.016735975751963394 1101 44-45 0.10860902037401843 1101 46-47 0.135862389353143 1101 48-49 -0.06974445758562808 1101 50-51 0.028898044055701178 1101 52-53 0.16581990123625445 1101 54-55 0.12026611615605276 1101 56-57 0.23169304178181704 1101 58-59 0.03932554563163393 1101 60-61 0.10426657282041418 1101 62-63 0.07281747245582437 1101 64-65 -0.1789870789216934 1101 66-67 -0.18543402759555505 1101 68-69 -0.14287874397991374 1101 70-71 -0.10708243537389706 1101 72-73 -0.10641381909075776 1101 74-75 -0.2327996628046769 1101 76-77 -0.18005199979922182 1101 78-79 -0.18489297804722327 1101 80-81 -0.08019201717582902 1101 82-83 -0.11342958751985677 1101 84-85 -0.20939838570748037 1101 86-87 -0.2052179848668061 1101 88-89 -0.25563514139476595 1101 90-91 -0.24581726310043805 1101 92-93 -0.04950562592327401 1101 94-95 -0.03679103121426763 1101 96-97 0.033086376222485114 1101 98-99 -0.24383835415680366 1101 100-101 -0.1574311755546809 1102 1 -0.056588314059609957 1102 2 0.0037021954778317934 1102 3 -0.08141912717508504 1102 4 -0.008872384312887505 1102 5 -0.0383189231881218 1102 6 0.004776152010741441 1102 7 -0.021278070886346256 1102 8 -0.0777811812341227 1102 9 -0.024824603154051772 1102 10-11 -0.017379751852317327 1102 12-13 0.00391136913109591 1102 14-15 0.03794349213733028 1102 16-17 -0.0017493980764911043 1102 18-19 -0.022941769591440675 1102 20-21 -0.009891661132776619 1102 22-23 -0.11637922304450399 1102 24-25 -0.061823966503176564 1102 26-27 -0.18171636961757542 1102 28-29 0.05625162021629393 1102 30-31 -0.04646137048623444 1102 32-33 -0.13291569216953292 1102 34-35 -0.18052580208198066 1102 36-37 -0.1371980832073305 1102 38-39 -0.1755467040637484 1102 40-41 -0.1183335332701887 1102 42-43 -0.1072716367205615 1102 44-45 -0.19528898022126384 1102 46-47 -0.17489436988997653 1102 48-49 -0.22708573046907787 1102 50-51 -0.15080335201749762 1102 52-53 -0.12410045474198483 1102 54-55 -0.24973494776176608 1102 56-57 -0.1309805661390655 1102 58-59 -0.45238683887505005 1102 60-61 -0.4113511906511107 1102 62-63 -0.4513316478573586 1102 64-65 -0.49600673260882644 1102 66-67 -0.5071771720848375 1102 68-69 -0.4593739880097729 1102 70-71 -0.352879872209769 1102 72-73 -0.160601705104785 1102 74-75 -0.13294892731443042 1102 76-77 -0.2348634891285002 1102 78-79 -0.3653293435307319 1102 80-81 -0.43640381664475925 1102 82-83 -0.5072254836061028 1102 84-85 -0.5087420806718264 1102 86-87 -0.5634571973663043 1102 88-89 -0.4408682078669237 1102 90-91 -0.49680876077017544 1102 92-93 -0.3186180200040525 1102 94-95 -0.3976591975173811 1102 96-97 -0.26872001344086627 1102 98-99 -0.3026455630017644 1102 100-101 -0.2931344199656287 1103 1 0.008990274319536695 1103 2 -0.07220263190287213 1103 3 0.054668811775894 1103 4 -0.04865639243207909 1103 5 0.04856729739299226 1103 6 0.0029409963266786576 1103 7 0.06053890721695154 1103 8 0.008763042082968298 1103 9 -0.07922260156514938 1103 10-11 0.033404448224366945 1103 12-13 0.01894634649552529 1103 14-15 0.12727909735689735 1103 16-17 -0.07887538571181807 1103 18-19 -0.05824134004567583 1103 20-21 -0.021833892669015142 1103 22-23 -0.03205605536285816 1103 24-25 -0.011041401101017811 1103 26-27 0.012074762880267542 1103 28-29 -0.07516506112352062 1103 30-31 0.07499990944524626 1103 32-33 0.04155090185530952 1103 34-35 -0.04999540624320531 1103 36-37 -0.0015171198329184676 1103 38-39 -0.15085920712876089 1103 40-41 0.06413601858145057 1103 42-43 0.08172353585202785 1103 44-45 0.17647208734264552 1103 46-47 0.24883702926531015 1103 48-49 0.348775602892907 1103 50-51 0.41843527263274183 1103 52-53 0.27717600421670596 1103 54-55 0.2023559527524057 1103 56-57 0.12121661157200947 1103 58-59 0.3348801791376559 1103 60-61 0.312978639496869 1103 62-63 0.17933168204938 1103 64-65 0.3280280271103635 1103 66-67 0.14323390185428764 1103 68-69 0.2597488783313011 1103 70-71 0.31446952405821804 1103 72-73 0.4382021685133921 1103 74-75 0.2679766604328009 1103 76-77 0.1762721005850061 1103 78-79 0.3158911418368291 1103 80-81 0.29267304995505583 1103 82-83 0.17225220518829687 1103 84-85 0.11031365892732836 1103 86-87 0.18561424911982272 1103 88-89 0.26592967957312297 1103 90-91 0.15726701993870051 1103 92-93 0.17076593655284 1103 94-95 0.11517317268128835 1103 96-97 0.09791092001900381 1103 98-99 0.1431251603510546 1103 100-101 0.3686083929542754 1104 1 -0.04639087495423411 1104 2 -0.06062927247121763 1104 3 -0.05029175555478815 1104 4 -0.022999128464512353 1104 5 -0.06255080505861343 1104 6 -0.11534048892375637 1104 7 -0.0985550060012983 1104 8 -0.0047264979159962195 1104 9 -0.18627023492597772 1104 10-11 -0.07663515193533499 1104 12-13 -0.03927138829204324 1104 14-15 -0.05634570697358754 1104 16-17 -0.03662536964178997 1104 18-19 -0.13709909634444983 1104 20-21 -0.029482997591330218 1104 22-23 -0.06384233581782439 1104 24-25 -0.03074551348797172 1104 26-27 0.06414661897252216 1104 28-29 -0.08918842014776374 1104 30-31 0.06030839957825407 1104 32-33 0.15324910952708848 1104 34-35 0.19108957837936913 1104 36-37 0.08583330446098358 1104 38-39 0.08991852316825799 1104 40-41 0.0692038476615835 1104 42-43 0.19171345474141077 1104 44-45 0.2398049559278732 1104 46-47 0.140021127456329 1104 48-49 0.0933229985984454 1104 50-51 0.08237350579928204 1104 52-53 0.14582608148862874 1104 54-55 0.034548125790799133 1104 56-57 0.036293892542111905 1104 58-59 0.04694507758868838 1104 60-61 0.027202126900228052 1104 62-63 0.04194825762240839 1104 64-65 -0.02819867701379053 1104 66-67 0.01637781412192396 1104 68-69 -0.021354086610699596 1104 70-71 -0.03915757081648508 1104 72-73 0.15876064031299109 1104 74-75 0.049811037789545765 1104 76-77 0.11342088003275919 1104 78-79 0.19660652496882491 1104 80-81 0.13981521126912 1104 82-83 0.11754441253958348 1104 84-85 0.12914240770170338 1104 86-87 0.07188386247052492 1104 88-89 0.16406474201082233 1104 90-91 0.04332127233856653 1104 92-93 -0.0825596529906889 1104 94-95 -0.10970632477512154 1104 96-97 -0.11724304438792998 1104 98-99 -0.11305749832797929 1104 100-101 -0.05167489666557046 1105 1 0.10733891610183122 1105 2 0.16377754122702726 1105 3 0.11343960499132777 1105 4 0.15266809835448214 1105 5 0.08801736676230831 1105 6 0.11250195961210352 1105 7 0.0798312626264277 1105 8 0.10952639549404353 1105 9 0.17216563334102375 1105 10-11 0.14889065655732026 1105 12-13 0.08079372091184922 1105 14-15 0.19944433889352808 1105 16-17 0.19812512249539083 1105 18-19 0.13155507883926276 1105 20-21 0.09838475281330261 1105 22-23 0.1925241281445551 1105 24-25 0.1489330624566989 1105 26-27 0.28724423398107746 1105 28-29 0.32326072098458525 1105 30-31 0.2067467480618177 1105 32-33 0.20814454606468047 1105 34-35 0.2462699981815959 1105 36-37 0.24500417031509159 1105 38-39 0.2745854499307825 1105 40-41 0.3293392578204788 1105 42-43 0.39426423970930813 1105 44-45 0.40440411074926885 1105 46-47 0.20362289363702502 1105 48-49 0.26500233653171534 1105 50-51 0.3815258756002251 1105 52-53 0.3565018513539897 1105 54-55 0.33716258184988845 1105 56-57 0.26985964256246575 1105 58-59 0.183176872735757 1105 60-61 0.3890788499082234 1105 62-63 0.37833402232418223 1105 64-65 0.38159587931905037 1105 66-67 0.31371002120733493 1105 68-69 0.39438341770304675 1105 70-71 0.5974640162171383 1105 72-73 0.5801266080912875 1105 74-75 0.4541190064588676 1105 76-77 0.5694491763665575 1105 78-79 0.5006741669402288 1105 80-81 0.634361291989709 1105 82-83 0.6174904539877311 1105 84-85 0.4776705474384002 1105 86-87 0.6034531343420966 1105 88-89 0.48608099244982483 1105 90-91 0.5468919795076168 1105 92-93 0.4894314949915781 1105 94-95 0.6136389130659623 1105 96-97 0.7173924104627183 1105 98-99 0.7830919959513594 1105 100-101 0.9238514983330788 1106 1 -0.1507630526008441 1106 2 -0.12870952260393054 1106 3 -0.018649359275908495 1106 4 -0.08488054928193378 1106 5 -0.004504393934539053 1106 6 -0.042166146703102925 1106 7 -7.09056509585082E-4 1106 8 0.06274712893819157 1106 9 -0.06835216414702217 1106 10-11 -0.17375380829049192 1106 12-13 -0.1888967504770065 1106 14-15 -0.10092480068682619 1106 16-17 -0.07886052151977196 1106 18-19 -0.018445547457950795 1106 20-21 -0.03985557747079582 1106 22-23 -0.05860301895888398 1106 24-25 -0.07448588153103941 1106 26-27 -0.08462224446240185 1106 28-29 -0.034736029785456424 1106 30-31 -0.013060277239340223 1106 32-33 0.02453248959271548 1106 34-35 -0.17512531686470112 1106 36-37 -0.1957929557322302 1106 38-39 0.07024395761482793 1106 40-41 -0.1542659720118138 1106 42-43 -0.19790297413236146 1106 44-45 0.00714259533546624 1106 46-47 -0.09351129555232518 1106 48-49 -0.0531609717205086 1106 50-51 0.22912033061518144 1106 52-53 0.03416132854612641 1106 54-55 0.0035488343754295215 1106 56-57 0.15460460807227605 1106 58-59 0.1413168576795698 1106 60-61 0.2145217359860183 1106 62-63 -0.008299980854907574 1106 64-65 0.09079793908534128 1106 66-67 -0.03294423642378774 1106 68-69 0.030259288099607318 1106 70-71 -0.009972887469622549 1106 72-73 0.004476041855976831 1106 74-75 0.1520571107573936 1106 76-77 0.06060548386847131 1106 78-79 0.19049971811309518 1106 80-81 0.1513880586802152 1106 82-83 0.04800302815697677 1106 84-85 -0.01787428730055396 1106 86-87 0.10146902475511155 1106 88-89 0.17337386030236246 1106 90-91 0.19384429198483488 1106 92-93 0.1696795212158939 1106 94-95 0.17853608132572418 1106 96-97 0.34088112396055337 1106 98-99 0.4544516746859415 1106 100-101 0.44811381415472695 1107 1 -0.06671691312641315 1107 2 -0.05854518610125936 1107 3 -0.0688345539235442 1107 4 -0.10084109175092948 1107 5 7.490098369089537E-6 1107 6 -0.0355583597496647 1107 7 -0.1437830911325335 1107 8 0.007634590598904367 1107 9 -0.13597487279725584 1107 10-11 -0.14438188476861313 1107 12-13 -0.05743475435777867 1107 14-15 -0.02557776543892487 1107 16-17 -0.1094469865227623 1107 18-19 -0.030735541182174586 1107 20-21 -0.050101355072136755 1107 22-23 -0.1845187399995396 1107 24-25 -0.012638402785519531 1107 26-27 0.009029123174194353 1107 28-29 -0.22130650267052232 1107 30-31 -0.07627630971096266 1107 32-33 -0.13480264393478336 1107 34-35 -0.1658321747659599 1107 36-37 -0.17315479345010232 1107 38-39 -0.2052680299226921 1107 40-41 -0.10650520916970407 1107 42-43 -0.060754971705897276 1107 44-45 -0.1460630342673781 1107 46-47 -0.2690492247525498 1107 48-49 -0.1990990052114583 1107 50-51 -0.12701637932689636 1107 52-53 -0.2791535573826138 1107 54-55 -0.4293789967050401 1107 56-57 -0.4605693648409641 1107 58-59 -0.3352379715967757 1107 60-61 -0.3023650417581365 1107 62-63 -0.4442629209624158 1107 64-65 -0.48867919229083867 1107 66-67 -0.39286056801064007 1107 68-69 -0.2182957088804116 1107 70-71 -0.1020474474767532 1107 72-73 -0.19090306672036306 1107 74-75 -0.1658677920596361 1107 76-77 -0.28957407698372606 1107 78-79 -0.4142964760615584 1107 80-81 -0.3104552929826063 1107 82-83 -0.28732034515009985 1107 84-85 -0.18674697793574424 1107 86-87 -0.07883732269808519 1107 88-89 -0.3381192789139611 1107 90-91 -0.14848994668632187 1107 92-93 -0.25014076070971214 1107 94-95 -0.08894067271717887 1107 96-97 -0.0934739571333445 1107 98-99 -0.10942390490714615 1107 100-101 -0.0022446524571755333 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-0.9075541650213168 2316 80-81 -1.0123222139454953 2316 82-83 -1.02554183906879 2316 84-85 -1.036007840028578 2316 86-87 -0.8884648535263295 2316 88-89 -1.0568164165696956 2316 90-91 -1.2599104409248731 2316 92-93 -1.267902309465999 2316 94-95 -1.3375800765373533 2316 96-97 -1.2068575688717083 2316 98-99 -1.385609214924159 2316 100-101 -1.876719112236838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 23.0 5 41.0 6 127.0 7 309.0 8 813.0 9 1563.0 10 2564.0 11 4155.0 12 6346.0 13 8928.0 14 11468.0 15 14850.0 16 18454.0 17 23325.0 18 21811.0 19 17418.0 20 16911.0 21 17334.0 22 18561.0 23 20623.0 24 24136.0 25 28963.0 26 36390.0 27 45656.0 28 56885.0 29 70993.0 30 88287.0 31 108699.0 32 134263.0 33 167744.0 34 218425.0 35 288032.0 36 405058.0 37 502125.0 38 398098.0 39 68307.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.199101852157646 19.051773142835863 13.515115402371217 19.23400960263528 2 13.889800003511791 25.693912310582785 36.670576460466016 23.74571122543941 3 27.99395186434414 24.815167555068562 28.698783100772634 18.492097479814664 4 12.13071037959695 15.795277011768317 42.19148508057819 29.882527528056546 5 11.636913739394625 42.092388642045194 33.67895352475357 12.591744093806614 6 27.02278367396531 39.7126520131882 16.616479913417756 16.648084399428733 7 27.215175871597424 34.95144028702149 20.899024521627922 16.934359319753163 8 23.2170103145276 30.20215041378557 18.650216611049288 27.93062266063754 9 22.431261941524674 23.677447062240077 24.00261822482531 29.88867277140994 10-11 25.425440557891044 24.73227308329861 27.433201491225713 22.409084867584628 12-13 32.21905439804532 22.509903498609944 24.954077474312005 20.31696462903273 14-15 24.13161672073148 26.28666494010438 29.590866192886274 19.990852146277867 16-17 19.857563793771252 28.532733604402942 30.11924230202923 21.490460299796574 18-19 18.976335896894756 33.97290685110356 28.056793285601767 18.993963966399914 20-21 22.601898410206566 27.612026132772822 25.69514281976517 24.090932637255445 22-23 20.27380746285376 28.29022474383992 26.404196885169277 25.03177090813704 24-25 24.627396337645653 28.1386303692308 26.527154425751593 20.706818867371954 26-27 24.661381850720396 24.17738326808963 31.494200761432108 19.66703411975787 28-29 20.003589190145952 31.58568102775863 29.19799159876647 19.21273818332895 30-31 21.678525886298292 28.373714148849157 26.638045565953618 23.309714398898937 32-33 22.277839331775265 28.510401064776854 27.87592470004864 21.335834903399245 34-35 25.383371647671044 25.842213058621986 28.69675413292652 20.077661160780448 36-37 24.539593945981107 24.80433390285352 30.778377620859715 19.877694530305657 38-39 22.003173380130974 27.41902630330364 31.889924166891138 18.687876149674253 40-41 21.62281428031332 29.28278313934468 28.461640714981467 20.63276186536053 42-43 19.52124340395915 30.819045057275424 26.1895347559162 23.470176782849222 44-45 22.464569160500062 26.240628064940125 26.17099367882711 25.123809095732707 46-47 24.899880799815854 29.394038515934007 25.568202468597363 20.13787821565278 48-49 24.60520323022083 28.03923544744921 27.196950835595075 20.158610486734876 50-51 19.91629476239153 29.041243712977106 30.739104220166862 20.3033573044645 52-53 22.18992945611787 31.74853945123328 26.08171207182139 19.979819020827456 54-55 19.839672356693804 28.266240736923535 27.71919117360453 24.174895732778133 56-57 23.372638159328258 26.6703526829288 27.32175568384234 22.635253473900597 58-59 27.38753177315307 25.89499026679704 25.492528864692588 21.224949095357296 60-61 22.96118242060344 27.52300569597959 30.700044636476115 18.815767246940858 62-63 22.120138959984644 27.121729065960686 29.605156754947547 21.15297521910712 64-65 20.573847346656873 32.31871594331506 27.269148766871794 19.838287943156274 66-67 22.20384063869563 27.30887470462873 26.233306200008606 24.253978456667028 68-69 20.485836826375955 29.607897223164997 26.373953996659395 23.532311953799656 70-71 24.073373709451378 28.752708743090533 25.467996711758833 21.705920835699256 72-73 23.662013722503985 25.44841840749706 29.010552611886446 21.87901525811251 74-75 20.679288177148756 32.30568477149001 27.07581931018063 19.93920774118061 76-77 22.68660558177721 27.50424811894101 25.82241791798453 23.98672838129725 78-79 22.398692151751693 28.30420078300935 27.160518321428533 22.136588743810425 80-81 24.441915335402115 27.76245368681241 27.067777820306766 20.72785315747871 82-83 23.497654799557964 26.153277820131187 29.12887488319648 21.22019249711437 84-85 21.169303474926252 28.220464759732273 29.464736731490827 21.145495033850654 86-87 22.15670183171484 29.215433853259142 26.277621277979463 22.350243037046557 88-89 21.191924377688764 29.612417745917714 25.748253609628158 23.447404266765364 90-91 22.94765551942933 25.053964015561863 26.849358313689038 25.149022151319773 92-93 23.69858669937575 29.137302645509706 26.342674220282746 20.8214364348318 94-95 22.848046880898345 28.461664337908793 27.294119134313082 21.396169646879777 96-97 18.77812009859823 28.531173288371026 30.332439811027673 22.358266802003076 98-99 21.605062586154617 30.954121278660484 25.735796197266264 21.705019937918635 100-101 19.8696911696875 28.454155841396723 26.6980522678593 24.978100721056474 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 231.0 1 175.5 2 166.5 3 235.0 4 215.5 5 193.0 6 328.0 7 663.0 8 1037.0 9 1230.0 10 1248.5 11 1251.5 12 1428.5 13 1612.5 14 1777.5 15 2088.0 16 2405.5 17 2685.0 18 3021.5 19 3381.5 20 3810.0 21 4324.5 22 5064.0 23 6162.5 24 7395.0 25 8989.5 26 11244.5 27 14006.5 28 17772.0 29 22481.5 30 27208.5 31 31751.5 32 36987.5 33 42243.0 34 47146.5 35 52472.0 36 57568.0 37 63014.0 38 67894.5 39 70609.0 40 72770.5 41 74379.0 42 77478.0 43 83713.5 44 88786.5 45 94437.5 46 109681.5 47 194267.5 48 402062.0 49 396899.5 50 193965.0 51 106314.5 52 71096.0 53 54900.0 54 45678.0 55 37842.5 56 30353.5 57 23075.0 58 17411.5 59 14071.0 60 11129.0 61 8196.0 62 5872.5 63 3992.5 64 2486.0 65 1497.5 66 877.5 67 493.0 68 259.0 69 139.0 70 73.5 71 41.0 72 26.5 73 21.0 74 11.0 75 10.0 76 10.0 77 4.5 78 2.5 79 1.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050180301439988535 2 0.006355937411223181 3 0.04333274456049395 4 0.0 5 0.0 6 0.0011939329943734152 7 0.0 8 0.0 9 0.0 10-11 2.2825189598315289E-4 12-13 0.0 14-15 0.002405423826899381 16-17 0.0 18-19 0.0 20-21 0.012237813192327507 22-23 0.0 24-25 0.0 26-27 0.00709336661363029 28-29 0.01025377748108933 30-31 0.010464471538919932 32-33 0.006531515792748683 34-35 0.008181952579088405 36-37 0.020981616592297515 38-39 0.010324008833699532 40-41 0.012518738602768309 42-43 0.0 44-45 0.0 46-47 1.755783815255022E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.001106143803610664 58-59 1.58020543372952E-4 60-61 0.009972852070648528 62-63 0.015327992707176344 64-65 0.041787654803069535 66-67 0.023825986373010654 68-69 0.0016328789481871709 70-71 2.8092541044080353E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 5.267351445765067E-5 86-87 5.969664971867076E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.4046270522040177E-4 96-97 4.389459538137556E-4 98-99 5.794086590341573E-4 100-101 0.0012114908325259654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2847731.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.697900070026606 #Duplication Level Percentage of deduplicated Percentage of total 1 76.25101187918418 27.22001002301529 2 13.681369316072466 9.767923093325663 3 4.106782582884445 4.39810542759404 4 1.7747646875723093 2.5342148985907316 5 0.9019791126063419 1.6099380113536237 6 0.5842594633050036 1.2514101561617637 7 0.39634549285676707 0.990409125804442 8 0.2908819388517161 0.8307099508243321 9 0.22231951975572464 0.7142705999870549 >10 1.4923099601354568 10.459656357084981 >50 0.17264488679826745 4.2839833649438495 >100 0.10939567473280837 7.295014169292363 >500 0.007324384522382569 1.7167306872106853 >1k 0.005047886630290688 3.9597483150845747 >5k 0.0016826288767635628 3.8881280647506165 >10k+ 0.0018805852152063351 19.079747754976044 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 131795 4.628070558630713 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 42343 1.4869030817868683 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 39247 1.378184947946277 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 36293 1.2744532401410107 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 31771 1.1156601518893463 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 27170 0.954092925209579 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 26876 0.9437689163758796 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 26200 0.9200307191936317 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 24838 0.8722031680660849 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 23718 0.8328736106043724 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 20393 0.7161139868899135 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 20076 0.7049823175011966 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 16934 0.5946488625505709 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 15943 0.5598492273322164 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 13640 0.4789778248015701 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11449 0.402039378017095 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 10814 0.37974092356335626 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 10317 0.3622884324397213 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 10179 0.3574424691096174 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 9361 0.3287178458920453 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8600 0.30199481622386387 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7385 0.2593292695131668 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7321 0.25708186622964035 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7201 0.2528679850730283 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 6965 0.2445806854650246 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6856 0.24075307674776866 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 6592 0.23148253820322212 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5979 0.20995662862819559 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5823 0.2044785831245999 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5772 0.20268768363303977 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5761 0.20230141119368367 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5687 0.19970285114710623 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 5350 0.18786886823228738 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5174 0.18168850920258972 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 5162 0.1812671210869285 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 5053 0.17743951236967256 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4938 0.173401209594586 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4875 0.17118892198736468 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 4747 0.16669411542031182 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4222 0.14825838536013408 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4116 0.14453612367179344 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3849 0.1351602380983316 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3686 0.12943638286060025 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3421 0.12013072863974862 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3410 0.11974445620039252 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3406 0.11960399349517212 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3346 0.11749705291686609 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 3229 0.11338851878916933 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3109 0.10917463763255729 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.511567630510045E-5 0.0 0.0 0.0 3 0.0 3.511567630510045E-5 0.0 0.0 0.0 4 0.0 3.511567630510045E-5 0.0 0.0 0.0 5 0.0 3.511567630510045E-5 0.0 0.0 0.0 6 0.0 3.511567630510045E-5 0.0 0.0 0.0 7 0.0 3.511567630510045E-5 0.0 0.0 0.0 8 0.0 3.511567630510045E-5 0.0 0.0 0.0 9 0.0 3.511567630510045E-5 0.0 0.0 0.0 10-11 0.0 3.511567630510045E-5 0.0 5.2673514457650676E-5 0.0 12-13 0.0 3.511567630510045E-5 0.0 1.0534702891530134E-4 0.0 14-15 0.0 3.511567630510045E-5 0.0 2.458097341357031E-4 0.0 16-17 0.0 3.511567630510045E-5 0.0 3.687146012035547E-4 0.0 18-19 0.0 3.511567630510045E-5 0.0 6.320821734918081E-4 0.0 20-21 0.0 3.511567630510045E-5 0.0 0.001018354612847913 0.0 22-23 0.0 3.511567630510045E-5 0.0 0.0017382259771024722 0.0 24-25 0.0 3.511567630510045E-5 0.0 0.002756580589950385 0.0 26-27 0.0 3.511567630510045E-5 0.0 0.0036520303357304466 0.0 28-29 0.0 3.511567630510045E-5 0.0 0.007356734185918543 0.0 30-31 0.0 3.511567630510045E-5 0.0 0.017031103007973718 0.0 32-33 0.0 3.511567630510045E-5 0.0 0.039698272062916054 0.0 34-35 0.0 3.511567630510045E-5 0.0 0.06399832006604557 0.0 36-37 0.0 8.778919076275112E-5 0.0 0.08001106846117137 0.0 38-39 0.0 1.404627052204018E-4 0.0 0.09583068063661912 0.0 40-41 0.0 1.404627052204018E-4 0.0 0.11949864646625682 0.0 42-43 0.0 1.404627052204018E-4 0.0 0.14787211292077798 0.0 44-45 0.0 1.404627052204018E-4 0.0 0.17417375447329822 0.0 46-47 0.0 1.404627052204018E-4 0.0 0.20495264475471875 0.0 48-49 0.0 1.404627052204018E-4 0.0 0.22705796298877948 0.0 50-51 0.0 1.404627052204018E-4 0.0 0.2623141019991003 0.0 52-53 0.0 1.404627052204018E-4 0.0 0.39519182113760043 0.0 54-55 0.0 1.404627052204018E-4 0.0 0.550613804463975 0.0 56-57 0.0 1.404627052204018E-4 0.0 0.6742561007342336 0.0 58-59 0.0 1.404627052204018E-4 0.0 0.889023577016228 0.0 60-61 0.0 1.5802054337295203E-4 0.0 1.129565959706166 0.0 62-63 0.0 1.7557838152550224E-4 0.0 1.2125794184914236 0.0 64-65 0.0 1.7557838152550224E-4 0.0 1.278105270476741 0.0 66-67 0.0 1.7557838152550224E-4 0.0 1.6661861671625586 0.0 68-69 0.0 1.7557838152550224E-4 0.0 1.9756957381157139 0.0 70-71 0.0 1.9313621967805244E-4 0.0 2.0454003555813385 0.0 72-73 0.0 2.1069405783060268E-4 0.0 2.1760657871126172 0.0 74-75 0.0 2.1069405783060268E-4 0.0 2.382967351902269 0.0 76-77 0.0 2.1069405783060268E-4 0.0 2.5474140640390543 0.0 78-79 0.0 2.1069405783060268E-4 0.0 2.7090515220714315 0.0 80-81 0.0 2.1069405783060268E-4 0.0 3.008061505809362 0.0 82-83 0.0 2.1069405783060268E-4 1.7557838152550224E-5 3.346190352951174 0.0 84-85 0.0 2.1069405783060268E-4 3.511567630510045E-5 3.730742124168329 0.0 86-87 0.0 2.1069405783060268E-4 3.511567630510045E-5 4.501829702313877 0.0 88-89 0.0 2.1069405783060268E-4 3.511567630510045E-5 5.463507613605358 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1940 0.0 32.329235 2 AGTACGC 1625 0.0 31.286257 2 TACGCAG 1625 0.0 31.271975 4 GTACGCA 1635 0.0 31.093811 3 ACCTGGG 2185 0.0 28.920658 3 GAGTACG 1865 0.0 28.550533 1 GTACTAG 485 0.0 25.486292 1 CGCAGTG 2025 0.0 25.329325 6 ACGCAGT 2115 0.0 24.02693 5 GTACCTG 3005 0.0 21.041767 1 ACGCGAT 115 0.0037280256 20.648907 7 GTACCGG 165 6.2843144E-5 20.16922 1 CCTGGGG 1715 0.0 19.661612 4 ACGTGCG 220 1.3477656E-6 19.429426 9 GCATCGT 565 0.0 18.492647 8 GAGTACT 8360 0.0 18.236391 12-13 TAACGCC 210 1.9601164E-5 18.092375 4 CTTTGCG 615 0.0 17.769527 2 AGTACTT 8485 0.0 17.63189 12-13 GTACTTT 8655 0.0 17.589233 14-15 >>END_MODULE