FastQCFastQC Report
Wed 25 May 2016
SRR1003794_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003794_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369652
Sequences flagged as poor quality0
Sequence length121
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT872086.367164798065494No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC538103.928735182367492No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT536433.9165423041765353No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT453443.310621968207983No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT285902.0873915417930977No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC204571.4935910727688493No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT147651.078011056823193No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA120970.8832170507544982No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC119280.870878150070237No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC116110.8477335848814151No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT112150.8188211312070511No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC102370.7474161319809703No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG99880.7292363315645142No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG96240.7026602377830281No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC91690.6694401205561705No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC87790.6409657343617211No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA83110.6067964709283817No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA80700.5892007604851451No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG63860.46624982112244573No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC50390.36790367188161666No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT43990.32117647402405863No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT43500.3175989229380894No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT43140.31497051805860177No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40390.2948924252291823No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA40120.29292112156956657No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT40020.2921910091030422No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG38610.281896423325049No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29360.21436102017154723No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27190.19851757964796896No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26540.19377184861556074No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26180.19114344373607312No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG25750.18800396013001844No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA22300.1628150800349286No Hit
GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC19530.14259096471220428No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG18390.13426768259382676No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC17790.12988700779468068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16490.12039554572986423No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16160.1179861745903339No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT15840.11564981469745599No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA15610.11397055602445001No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA15020.10966289247195639No Hit
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT14510.10593931889268224No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC14390.10506318393285301No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14150.10331091401319459No Hit
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13890.1014126216002313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1500.057.5517122
ATAACGC2050.042.064333
TACCTGG8500.037.239342
ACCTGGG8850.035.0773743
TCTCGGG4150.030.464268114-115
GTAGGCA7150.029.7489933
GTACCCG850.00843992627.1112381
GTATAAC4000.025.9251231
TCACGCA2008.540883E-825.8686074
AGTACGC10300.025.702712
GAGTACG10800.025.6050591
GAGTAGG9150.025.1853051
GTACGCA10650.024.8304623
TAGGCAG8200.024.536624
GGCAGTG9550.023.481086
AGTAGGC8850.023.4108662
GTACACG1250.001970898823.0445541
CGTTATA1502.1137157E-423.0206852
TAAGACG1752.285528E-522.9943184
TACGCAG11700.022.6012524
Produced by FastQC (version 0.11.5)