##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003794_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1369652 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.961140494081707 31.0 31.0 34.0 30.0 34.0 2 32.10695417522115 33.0 31.0 34.0 30.0 34.0 3 32.21596580737297 34.0 31.0 34.0 30.0 34.0 4 35.74974665097412 37.0 35.0 37.0 35.0 37.0 5 35.686399903041064 37.0 35.0 37.0 35.0 37.0 6 35.877708352194574 37.0 35.0 37.0 35.0 37.0 7 35.881638547601874 37.0 35.0 37.0 35.0 37.0 8 35.911355585214345 37.0 35.0 37.0 35.0 37.0 9 37.5167809049306 39.0 37.0 39.0 35.0 39.0 10-11 37.41708769818903 39.0 37.0 39.0 34.5 39.0 12-13 37.43519010668403 39.0 37.0 39.0 34.0 39.0 14-15 38.3611472111164 40.0 37.5 41.0 34.0 41.0 16-17 38.55269112154036 40.0 38.0 41.0 34.5 41.0 18-19 38.665879727113165 40.0 38.0 41.0 34.0 41.0 20-21 38.61404064682124 40.0 38.0 41.0 34.0 41.0 22-23 38.52953779500194 40.0 38.0 41.0 34.0 41.0 24-25 38.28358371323519 40.0 37.5 41.0 33.5 41.0 26-27 37.89986726555358 39.5 37.5 41.0 33.5 41.0 28-29 37.83344017312427 39.5 37.0 41.0 33.0 41.0 30-31 37.8539727609641 40.0 37.0 41.0 33.0 41.0 32-33 37.59383186386031 39.0 37.0 41.0 33.0 41.0 34-35 37.424497609611784 39.0 36.5 41.0 32.5 41.0 36-37 37.2026401596902 39.5 36.0 40.5 31.5 41.0 38-39 37.02583466457173 39.0 36.0 40.0 31.0 41.0 40-41 37.35534610251363 39.5 37.0 41.0 32.5 41.0 42-43 37.283862981253634 40.0 37.0 41.0 31.5 41.0 44-45 37.03404879487636 39.5 36.5 41.0 31.0 41.0 46-47 36.91096132448242 39.5 36.5 41.0 31.0 41.0 48-49 36.513429323653014 39.0 36.0 41.0 30.0 41.0 50-51 36.072246453843746 39.0 35.0 40.0 28.5 41.0 52-53 36.14009178973929 39.0 35.0 40.0 29.0 41.0 54-55 36.03475773408135 39.0 35.0 40.5 28.5 41.0 56-57 35.567168886695306 38.5 34.5 40.0 28.0 41.0 58-59 35.28363847167017 38.0 34.0 40.0 28.0 41.0 60-61 34.51808926647061 38.0 34.0 40.0 26.0 41.0 62-63 34.51073520865154 37.0 34.0 40.0 26.0 41.0 64-65 34.06834144731654 37.0 33.5 39.5 26.0 40.5 66-67 33.61009767444577 36.5 33.0 39.0 25.5 40.0 68-69 33.07762300204723 36.0 32.5 38.0 24.5 40.0 70-71 32.32025908770987 35.0 31.5 37.0 23.0 39.0 72-73 31.68545805795925 34.5 31.0 37.0 21.5 39.0 74-75 31.351189572241708 34.0 31.0 36.0 20.0 39.0 76-77 30.529522462640145 34.0 30.0 35.0 20.0 37.0 78-79 30.982854038836145 34.0 31.0 35.0 20.0 37.0 80-81 30.555300178439488 34.0 30.5 35.0 19.5 37.0 82-83 30.20546277448578 34.0 30.0 35.0 15.5 36.0 84-85 29.917154503479715 34.0 29.5 35.0 16.0 36.0 86-87 29.94642726765631 34.0 30.0 35.0 13.0 36.0 88-89 29.625534442325495 34.0 30.0 35.0 7.5 35.0 90-91 29.244055424297557 34.0 29.0 35.0 4.0 35.0 92-93 29.008585027437626 34.0 29.0 35.0 2.0 35.0 94-95 28.464618384815996 34.0 29.0 35.0 2.0 35.0 96-97 27.814566765864612 33.0 28.0 35.0 2.0 35.0 98-99 27.746284822714088 34.0 29.0 35.0 2.0 35.0 100-101 26.622899101377577 33.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.021344028320669395 1101 2 0.09709491004201709 1101 3 0.020447061075095974 1101 4 0.020762933453511323 1101 5 -0.03736006025864924 1101 6 -0.0513555189183208 1101 7 0.011051289380716867 1101 8 0.010998981813045816 1101 9 0.03801541231378991 1101 10-11 0.10168434253819214 1101 12-13 0.03203877246426856 1101 14-15 0.11581515450757252 1101 16-17 0.057731969924994075 1101 18-19 0.08871780655433525 1101 20-21 -0.03177003229390607 1101 22-23 0.04102057656132985 1101 24-25 0.017522906031622654 1101 26-27 -0.01565808155402948 1101 28-29 -3.7495857792890774E-4 1101 30-31 0.2198256205224567 1101 32-33 0.13419769798425563 1101 34-35 0.12340019840248573 1101 36-37 0.23151977462939755 1101 38-39 0.21301423624006333 1101 40-41 0.2784620420967201 1101 42-43 0.2814551546884587 1101 44-45 0.45747699739573733 1101 46-47 0.25579804879499335 1101 48-49 0.36167672588252486 1101 50-51 0.2018912893242728 1101 52-53 0.22816543128158884 1101 54-55 0.16778917591726383 1101 56-57 0.07197547031794471 1101 58-59 -0.12466495181378434 1101 60-61 -0.042470570412667996 1101 62-63 0.023821929346219406 1101 64-65 -0.06550045737437671 1101 66-67 -0.06402019616317034 1101 68-69 0.08268110562619313 1101 70-71 0.14818198206032918 1101 72-73 0.04019422445134779 1101 74-75 -0.041586749292601866 1101 76-77 0.040426554007918725 1101 78-79 0.04737363963675634 1101 80-81 -0.04991841459004931 1101 82-83 0.16625831701817972 1101 84-85 0.16241480050691948 1101 86-87 -0.10863694323732886 1101 88-89 -0.03139385416709217 1101 90-91 0.19954619452635214 1101 92-93 0.05372949931758697 1101 94-95 0.11042199850096779 1101 96-97 -0.1489206964612464 1101 98-99 -0.2185218439609713 1101 100-101 -0.14238024838290286 1102 1 -0.03980175024901911 1102 2 0.027055625796137406 1102 3 -0.038160749476027434 1102 4 -0.12692579158785833 1102 5 -0.011867106891848778 1102 6 -0.06784799202917213 1102 7 0.018818997523304404 1102 8 -0.04852926172854666 1102 9 0.08756262423796102 1102 10-11 0.06849347362042835 1102 12-13 -0.06681828747097995 1102 14-15 -0.13803432152973016 1102 16-17 -0.002265127808819045 1102 18-19 -0.01373460837299234 1102 20-21 -0.054414273195817486 1102 22-23 -0.06078055601349064 1102 24-25 -0.06621922070757336 1102 26-27 -0.3767086328118481 1102 28-29 -0.03202417133822166 1102 30-31 -0.08429421543039695 1102 32-33 -0.08891712464927792 1102 34-35 0.08582794406722627 1102 36-37 0.032660987154748966 1102 38-39 -0.02767933627685437 1102 40-41 0.09789651479987072 1102 42-43 -0.009723338137064275 1102 44-45 0.2470775557364746 1102 46-47 0.04515122349413758 1102 48-49 -0.013015167466100763 1102 50-51 0.11568154531105534 1102 52-53 0.10881802656401618 1102 54-55 0.24374770115142752 1102 56-57 0.21604292727629826 1102 58-59 0.12382431498394197 1102 60-61 -0.15307593476800463 1102 62-63 0.06354531573804678 1102 64-65 -0.22397801145499585 1102 66-67 -0.12175870888995632 1102 68-69 -0.25885412408472064 1102 70-71 -0.18358712193408877 1102 72-73 -0.3579573083943579 1102 74-75 -0.20737905068603268 1102 76-77 -0.13201159144398744 1102 78-79 -0.1406230711282319 1102 80-81 -0.22604658419296086 1102 82-83 -0.33340723135464856 1102 84-85 -0.2931635969584363 1102 86-87 -0.27134593231582826 1102 88-89 -0.4793672777010123 1102 90-91 -0.23179344317408734 1102 92-93 -0.17198872518652664 1102 94-95 -0.46236649194055346 1102 96-97 -0.1756364022129162 1102 98-99 -0.12426487593081958 1102 100-101 0.04214652675020503 1103 1 -0.11402731641597086 1103 2 -0.13087071152115826 1103 3 -0.061362805876946425 1103 4 -0.03388115492914068 1103 5 0.050339021032804965 1103 6 -0.007634471568358947 1103 7 0.016158435015093175 1103 8 0.10802363993570907 1103 9 0.10281676612211754 1103 10-11 0.12681570564099331 1103 12-13 -0.04895236631219291 1103 14-15 -0.07665187974126297 1103 16-17 0.020799960282445795 1103 18-19 -0.07593135490656522 1103 20-21 0.04593471455208231 1103 22-23 0.12360880924158124 1103 24-25 0.12027532870421709 1103 26-27 -0.10555265780387657 1103 28-29 -0.03976944780782077 1103 30-31 0.004992235964614622 1103 32-33 -0.1283433587712679 1103 34-35 -0.2159657779058719 1103 36-37 -0.20284570177708616 1103 38-39 -0.27063860227681147 1103 40-41 -0.1727978957673244 1103 42-43 -0.2080065435707681 1103 44-45 -0.2331633583862711 1103 46-47 -0.17410126726519337 1103 48-49 -0.20786666004542553 1103 50-51 -0.34844401522705226 1103 52-53 -0.29355734165557834 1103 54-55 -0.38905151831683327 1103 56-57 -0.5518239320215983 1103 58-59 -0.6751868863969932 1103 60-61 -0.6599956488232053 1103 62-63 -0.5525756666812285 1103 64-65 -0.6697910966192708 1103 66-67 -0.5541413945945948 1103 68-69 -0.8252180845327786 1103 70-71 -0.7211314490069078 1103 72-73 -0.6927622692739597 1103 74-75 -0.8552466604952151 1103 76-77 -0.8765075293016551 1103 78-79 -0.7314931013363122 1103 80-81 -0.6511192899737708 1103 82-83 -0.6145506352985883 1103 84-85 -0.6635915760191722 1103 86-87 -0.5580750165044286 1103 88-89 -0.6304695911520533 1103 90-91 -0.741893448802923 1103 92-93 -0.4977500250720688 1103 94-95 -0.5921730476566367 1103 96-97 -0.485738284119968 1103 98-99 -0.2899815203948606 1103 100-101 -0.3705802683789763 1104 1 0.09181126657961869 1104 2 0.06600465355877816 1104 3 0.12742733044951393 1104 4 -0.03177649718315223 1104 5 -0.18535299079353962 1104 6 0.08686394229973615 1104 7 0.047258302912318584 1104 8 0.034920482949090115 1104 9 0.0786904866573579 1104 10-11 -0.011557003903661212 1104 12-13 0.00604417385334699 1104 14-15 -0.0381538073633223 1104 16-17 0.06174867385308147 1104 18-19 -0.029845910475096105 1104 20-21 0.025015297242120482 1104 22-23 0.061502084581498195 1104 24-25 0.05570538163518535 1104 26-27 -0.010178501419353836 1104 28-29 -0.17828279737691588 1104 30-31 -0.045369923331556095 1104 32-33 0.09223681502924563 1104 34-35 -0.06848067316724382 1104 36-37 -0.1458613294449762 1104 38-39 0.143410122976384 1104 40-41 0.318495453262571 1104 42-43 0.06673711414825334 1104 44-45 0.37678299171302143 1104 46-47 0.33912159100740524 1104 48-49 0.2861693941257002 1104 50-51 0.008667517254949075 1104 52-53 0.10723033234713597 1104 54-55 0.1708642774001632 1104 56-57 0.048723350885019556 1104 58-59 0.1319241967558611 1104 60-61 -0.04941799126593338 1104 62-63 0.1769088629160862 1104 64-65 0.23221470871337857 1104 66-67 0.10103038394005637 1104 68-69 0.031599586684095016 1104 70-71 -0.11314372467932543 1104 72-73 0.10241238496725558 1104 74-75 -0.055454699892397485 1104 76-77 -0.05291909683133511 1104 78-79 0.19026925746200263 1104 80-81 0.09125244901990825 1104 82-83 0.12274188696567023 1104 84-85 0.38682315478773077 1104 86-87 0.3012683338734554 1104 88-89 0.24745037433946848 1104 90-91 0.13679352155034152 1104 92-93 0.03442913144724358 1104 94-95 -0.037293333070511636 1104 96-97 -0.1552823048969465 1104 98-99 0.008632299172525393 1104 100-101 0.15055078296137125 1105 1 0.0040130077958231425 1105 2 0.02156704346510452 1105 3 0.047745414362118765 1105 4 -0.021961487626086296 1105 5 -0.17761363970411992 1105 6 -0.1989056166200811 1105 7 0.011689916414354684 1105 8 -0.041197649906280276 1105 9 -0.07659588366303893 1105 10-11 -0.013432041106781867 1105 12-13 -0.10214194535978294 1105 14-15 0.06454058031191323 1105 16-17 0.02587329272780181 1105 18-19 0.006549763160400346 1105 20-21 -0.11627773987721923 1105 22-23 0.08605559203645186 1105 24-25 -0.045162470681106015 1105 26-27 0.07190150016797503 1105 28-29 -0.09360133323851727 1105 30-31 0.05131574723950649 1105 32-33 0.0893057604007339 1105 34-35 -0.22165280400758292 1105 36-37 0.012656476243407155 1105 38-39 -0.1312447974254738 1105 40-41 0.1145942515191507 1105 42-43 0.07323140085146917 1105 44-45 0.088248827675379 1105 46-47 0.05562390256121574 1105 48-49 -0.13774278667154505 1105 50-51 -0.21479150199366615 1105 52-53 -0.11818253960138492 1105 54-55 -0.18103699489139302 1105 56-57 -0.3097967696641106 1105 58-59 -0.10284897996060494 1105 60-61 -0.3158733442160653 1105 62-63 -0.3496122761717686 1105 64-65 -0.7536855847227812 1105 66-67 -0.3592107804569409 1105 68-69 -0.47195648034544746 1105 70-71 -0.6433576178121285 1105 72-73 -0.6217218019985324 1105 74-75 -0.7771329298396417 1105 76-77 -0.7561357611831383 1105 78-79 -0.5558395718578026 1105 80-81 -0.7570756703651753 1105 82-83 -0.6142215889410352 1105 84-85 -0.553597841219684 1105 86-87 -0.397212786783701 1105 88-89 -0.28140271136990336 1105 90-91 -0.24848473573430496 1105 92-93 -0.2535759215630655 1105 94-95 -0.383640041889695 1105 96-97 -0.23159609860096708 1105 98-99 -0.0905379885977311 1105 100-101 -0.06868196195640408 1106 1 -0.023333671508783738 1106 2 -0.12549368336882694 1106 3 0.016085847021301447 1106 4 0.03115212823377078 1106 5 -0.02354494563748233 1106 6 -0.047010496312204 1106 7 -0.10074589464080219 1106 8 0.03126495967647713 1106 9 -0.01043701864158919 1106 10-11 0.055829210277877905 1106 12-13 -0.03270749326404854 1106 14-15 -0.01455123890460186 1106 16-17 0.0626622657427447 1106 18-19 0.01546674482396071 1106 20-21 0.021243624715992837 1106 22-23 -0.034556815225030846 1106 24-25 -0.056346288024776925 1106 26-27 0.037350813265376814 1106 28-29 -0.11992213140829477 1106 30-31 -0.038938952971001584 1106 32-33 -0.013008277231399745 1106 34-35 -0.0785319175244652 1106 36-37 -0.12307007218998933 1106 38-39 -0.24000723076223807 1106 40-41 -0.10554004564759367 1106 42-43 0.03537277069780487 1106 44-45 0.05558812215846132 1106 46-47 -0.08537099277607041 1106 48-49 -0.1954414631135748 1106 50-51 -0.2836264131664521 1106 52-53 -0.5121266052882234 1106 54-55 -0.5259081602086226 1106 56-57 -0.33148111802973546 1106 58-59 -0.19524109114404808 1106 60-61 -0.5438873220844371 1106 62-63 -0.29008452408860563 1106 64-65 -0.3805922200229901 1106 66-67 -0.26926441557823466 1106 68-69 -0.3929750527909306 1106 70-71 -0.5426804220648727 1106 72-73 -0.6039906171153397 1106 74-75 -0.6585056691897009 1106 76-77 -0.44788607808333936 1106 78-79 -0.5545358082634522 1106 80-81 -0.6141317912021726 1106 82-83 -0.6444765175480853 1106 84-85 -0.576279521152383 1106 86-87 -0.2835512476194033 1106 88-89 -0.521944239571166 1106 90-91 -0.3214383978825275 1106 92-93 -0.21762630584024834 1106 94-95 -0.22564292729934365 1106 96-97 -0.5400894046789801 1106 98-99 -0.418698140253035 1106 100-101 -0.32236390454512787 1107 1 0.04713545788847284 1107 2 0.03454754344980415 1107 3 0.03027534812937205 1107 4 0.02352879296182664 1107 5 -0.04807601846920306 1107 6 -0.08400863720025598 1107 7 0.03738486801350405 1107 8 -0.07034324886762988 1107 9 0.06705294313015742 1107 10-11 0.052211403527891775 1107 12-13 0.09465004339168814 1107 14-15 0.1110957195017761 1107 16-17 0.04175039434364436 1107 18-19 0.13909838682566544 1107 20-21 0.023324491204419928 1107 22-23 0.056550827775261325 1107 24-25 0.3062481842900766 1107 26-27 -0.16309436943107158 1107 28-29 -0.047774501462228613 1107 30-31 -0.0209928741909593 1107 32-33 -0.001150685485690417 1107 34-35 -0.1947040994636069 1107 36-37 0.01837585108295059 1107 38-39 -0.1522719303685207 1107 40-41 0.19306987417210308 1107 42-43 0.16476933336298316 1107 44-45 0.31387846439962885 1107 46-47 0.21849339386342592 1107 48-49 0.024719650624184908 1107 50-51 -0.22736561309280745 1107 52-53 -0.20276995844000822 1107 54-55 0.03778978500545094 1107 56-57 -0.036422757534957384 1107 58-59 0.2068612534569283 1107 60-61 -0.23553205736669725 1107 62-63 0.06525690119336502 1107 64-65 -0.187188789940528 1107 66-67 -0.08934129048496686 1107 68-69 -0.020378613973562665 1107 70-71 0.03011922201211803 1107 72-73 0.0033309088348225657 1107 74-75 -0.222872364726296 1107 76-77 -0.1736315010836087 1107 78-79 0.017451433179822118 1107 80-81 0.12135618733087128 1107 82-83 -0.032542078789298046 1107 84-85 -0.1821903256192705 1107 86-87 -0.19327274344442102 1107 88-89 -0.15156046475104645 1107 90-91 -0.13639190613695362 1107 92-93 -0.27891702882977754 1107 94-95 -0.41868792394512866 1107 96-97 -0.5300651232543387 1107 98-99 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90-91 -0.281787460093021 2316 92-93 -0.3488167592677769 2316 94-95 -0.4306930748711295 2316 96-97 -0.5765040503752985 2316 98-99 -0.3999563274262812 2316 100-101 -0.3616631747650345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 12.0 6 29.0 7 116.0 8 255.0 9 534.0 10 930.0 11 1621.0 12 2666.0 13 3950.0 14 4645.0 15 6159.0 16 7792.0 17 10238.0 18 9825.0 19 7730.0 20 7653.0 21 7717.0 22 8340.0 23 8642.0 24 9481.0 25 11673.0 26 14044.0 27 17542.0 28 21769.0 29 27174.0 30 33933.0 31 43133.0 32 54717.0 33 72265.0 34 98754.0 35 141549.0 36 222186.0 37 277421.0 38 200449.0 39 34692.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.78907631038402 18.033247917439745 12.480460018641509 15.697215753534726 2 14.367896238999597 30.8631879609436 32.37967916390737 22.38923663614944 3 37.5312381065087 23.300102781679875 24.729915020662258 14.438744091149161 4 12.884513730495046 16.003627198733696 44.97375975795311 26.13809931281815 5 11.655588426841271 43.77287077301388 33.34540452611321 11.226136274031653 6 26.40575854304597 43.42555627268824 13.744951272293985 16.423733911971798 7 29.33482373624833 35.51507974288359 19.09930405679691 16.050792464071165 8 23.079220123067756 25.409812127460114 18.424607126481764 33.08636062299036 9 19.399964370511633 31.797931153314856 22.62078250533712 26.181321970836386 10-11 27.34544979308613 23.440187726517394 26.72226229728427 22.492100183112207 12-13 37.68454968289379 22.155745457877423 20.930981348175067 19.228723511053726 14-15 23.927340509768573 27.979518428996624 31.785788993528502 16.307352067706297 16-17 18.623124706129733 28.544586508105706 30.60233548375792 22.229953302006642 18-19 17.714134685306927 39.57523516922547 25.275872995476224 17.434757149991384 20-21 24.379479750214678 25.902631332986736 22.768120534853697 26.94976838194489 22-23 19.717052214723154 30.017588409318574 23.282154883138197 26.98320449282007 24-25 29.168139060140824 28.640559791830334 23.51367354627307 18.677627601755773 26-27 28.314521337319526 22.224647980136695 32.41448625307169 17.046344429472082 28-29 19.614403601995818 37.54716264696454 26.618791453292275 16.219642297747367 30-31 23.571213164502982 27.835795665720582 23.55770459459321 25.035286575183225 32-33 23.67855176633629 30.776717743972874 24.892848641678555 20.65188184801228 34-35 29.175340383291264 24.812549149032645 27.322152605327698 18.68995786234839 36-37 26.568928040813034 23.204489083090614 31.513404190916017 18.71317868518034 38-39 21.169870957868735 29.35294854567827 31.990403014233674 17.486777482219313 40-41 20.751695130895538 31.628022951486383 26.090759642796773 21.529522274821307 42-43 18.225969158242815 34.52357090256153 21.10981255948906 26.140647379706593 44-45 25.126050113362957 23.360508800689306 23.23458647165561 28.278854614292133 46-47 29.630490932488797 31.045077441200363 22.812489161882684 16.51194246442816 48-49 28.602995828094723 28.780305452973703 24.745005614622258 17.871693104309312 50-51 17.885652934354095 30.10371060473698 34.51822884818767 17.49240761272126 52-53 24.482365112660744 36.02300015405474 21.289671834193292 18.204962899091225 54-55 19.086160659852705 29.965523762100815 24.517226802098946 26.43108877594753 56-57 26.04127304371446 24.58058276459951 26.449673400197664 22.928470791488362 58-59 34.5366592736599 24.496408459060085 21.13286419685478 19.834068070425236 60-61 23.49503660438099 28.733991858067075 32.243762944238604 15.527208593313336 62-63 19.917565668181737 27.62178047934578 30.395852728036072 22.06480112443641 64-65 18.57608845352818 38.561570747150434 24.769238244183146 18.09310255513824 66-67 23.93121349583295 25.973913100636654 22.707207822418024 27.38766558111238 68-69 20.12927664742464 30.521579640571193 22.666391102389778 26.682752609614397 70-71 28.867101441973126 28.689109367823267 22.81478674176289 19.629002448440716 72-73 27.536175273847135 23.01327126399353 31.043382584322575 18.407170877836762 74-75 20.020706027344954 37.219522220830946 25.76378117175234 16.995990580071762 76-77 23.69305454556074 27.582751430298675 23.251370429104202 25.472823595036374 78-79 23.32759461582718 30.91712152939012 24.191906802059513 21.56337705272319 80-81 28.16404310621769 26.15413539324202 25.746729067492186 19.935092433048098 82-83 25.41906038916023 24.245249578083026 30.00332568778034 20.332364344976405 84-85 20.555437925294235 29.50344616103502 30.376339768114246 19.564776145556497 86-87 21.095546842485707 31.03830343387437 24.8736118108339 22.99253791280602 88-89 18.770525316686744 33.199978096594016 20.904209104515754 27.12528748220348 90-91 24.54109274739813 22.47098597842717 23.479499532541226 29.508421741633473 92-93 27.96905773200769 30.10099313874555 23.301980984158334 18.627968145088424 94-95 26.94086547711053 28.290113750986134 24.854584994536786 19.914435777366553 96-97 17.251974054810255 29.718866265156567 33.63912062059924 19.390039059433935 98-99 23.53898551608243 35.77771879667351 20.96880479659977 19.71449089064429 100-101 18.186786687895275 31.10218756651434 23.194068353832375 27.516957391758005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 76.0 1 52.0 2 73.0 3 123.5 4 111.0 5 96.5 6 126.5 7 223.0 8 337.0 9 438.5 10 516.5 11 542.0 12 581.5 13 688.0 14 767.0 15 817.5 16 937.0 17 1100.0 18 1206.5 19 1299.5 20 1397.0 21 1460.0 22 1635.0 23 1935.0 24 2250.0 25 2649.0 26 3284.0 27 4270.0 28 5845.0 29 8194.5 30 11029.5 31 13922.5 32 16566.0 33 18827.0 34 20625.5 35 21820.5 36 22934.5 37 23918.5 38 24969.0 39 25538.5 40 25279.0 41 25456.0 42 26087.5 43 27481.0 44 28198.0 45 29816.5 46 39405.0 47 112069.0 48 268726.0 49 262051.0 50 113718.0 51 47794.0 52 26794.5 53 19329.0 54 15587.0 55 13158.0 56 10942.5 57 8559.0 58 6608.0 59 5213.0 60 3998.0 61 3038.0 62 2282.5 63 1657.0 64 1134.5 65 773.5 66 515.0 67 332.0 68 212.0 69 128.5 70 73.5 71 35.5 72 25.5 73 15.0 74 8.5 75 2.5 76 0.5 77 1.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0484794677772164 2 0.0031394836060546767 3 0.053371221302929504 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.650562332621717E-5 14-15 0.0022268430228992477 16-17 0.0 18-19 0.0 20-21 0.011389754477779757 22-23 0.0 24-25 0.0 26-27 0.006680529068697743 28-29 0.013799125617310089 30-31 0.011024698244517586 32-33 0.006863057185328828 34-35 0.006790045938676393 36-37 0.020844710919270006 38-39 0.04968415334698156 40-41 0.004928259149039318 42-43 0.012959496280807095 44-45 0.011462765724432191 46-47 0.004709225409082015 48-49 0.0010221574531340807 50-51 0.001715764296332207 52-53 6.571012198719091E-4 54-55 9.491462064816463E-4 56-57 0.01186432758102058 58-59 0.008359787741703732 60-61 0.005512349122258793 62-63 0.005621865992237444 64-65 0.071441504849407 66-67 0.03351216221346736 68-69 0.024860329485153893 70-71 0.015259350550358777 72-73 7.301124665243434E-5 74-75 1.8252811663108586E-4 76-77 5.840899732194747E-4 78-79 5.840899732194747E-4 80-81 8.761349598292122E-4 82-83 7.666180898505606E-4 84-85 8.761349598292122E-4 86-87 0.0045266972924509295 88-89 1.4602249330486868E-4 90-91 9.126405831554293E-4 92-93 3.2855060993595454E-4 94-95 0.0027379217494662876 96-97 0.00529331538230149 98-99 0.007191607795264783 100-101 0.0085058102350086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1369652.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.77571768204159 #Duplication Level Percentage of deduplicated Percentage of total 1 84.04834998559156 29.228416907403563 2 9.677025675808462 6.7305102580756575 3 2.521241563651445 2.630339544773152 4 1.0465882321603897 1.4558342756382685 5 0.5958844184389401 1.036115415338006 6 0.3869459939887599 0.8073794787090045 7 0.2627207852851239 0.6395412700795814 8 0.18850268242837945 0.5244252853149493 9 0.1528105090825551 0.478268561043359 >10 0.9604603500506497 6.292829584635173 >50 0.0856013486964621 2.0754029638732048 >100 0.05561860127679717 3.6981308843991596 >500 0.005866024352297366 1.4152734124510713 >1k 0.007604105641866955 6.156575340966688 >5k 0.00173808128956959 4.431229214192576 >10k+ 0.0030416422567467825 32.399727603106605 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 138024 10.077304307955599 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 45751 3.3403375455955238 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 30598 2.233998125071186 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 27045 1.9745891657150867 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 25060 1.8296618411100047 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 24513 1.7897246891911232 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 23265 1.698606653368885 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 22870 1.6697672109411734 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 22494 1.6423149821998582 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 18789 1.371808313352589 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16058 1.1724145987447907 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 12116 0.8846042644408945 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 11453 0.8361978079103305 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10794 0.7880833963663761 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 9876 0.7210590719394416 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 8345 0.6092788533145646 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 8173 0.596720918890346 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7052 0.5148753113929669 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6689 0.48837222885813336 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6266 0.45748847152415356 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6184 0.45150154929865394 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6065 0.44281321094701426 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 4847 0.35388551252434924 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4511 0.32935373364913134 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 4477 0.3268713512629486 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4067 0.29693674013545046 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3668 0.26780525272112915 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3470 0.2533490258839472 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3424 0.24999050853793517 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3300 0.24093711395303335 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2993 0.218522661230736 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2763 0.2017300745006761 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2754 0.20107297328080417 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2500 0.18252811663108584 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 2486 0.18150595917795176 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2480 0.18106789169803716 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2476 0.18077584671142743 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2292 0.1673417773273795 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 2288 0.16704973234076978 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2222 0.1622309900617091 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2089 0.15252049425693534 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 2007 0.14653357203143574 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 1967 0.14361312216533836 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1903 0.13894040237958255 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1746 0.12747763665515036 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1538 0.11229129735144401 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 1452 0.10601233013933466 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.301124665243434E-5 0.0 10-11 0.0 0.0 0.0 1.4602249330486868E-4 0.0 12-13 0.0 0.0 0.0 3.650562332621717E-4 0.0 14-15 0.0 0.0 0.0 6.936068431981262E-4 0.0 16-17 0.0 0.0 0.0 0.0013142024397438182 0.0 18-19 3.650562332621717E-5 0.0 0.0 0.001496730556374904 0.0 20-21 7.301124665243434E-5 0.0 0.0 0.0027379217494662876 0.0 22-23 7.301124665243434E-5 0.0 0.0 0.0045632029157771465 0.0 24-25 7.301124665243434E-5 0.0 0.0 0.007009079678633697 0.0 26-27 7.301124665243434E-5 0.0 0.0 0.009454956441490248 0.0 28-29 7.301124665243434E-5 0.0 0.0 0.0170116204700172 0.0 30-31 7.301124665243434E-5 0.0 0.0 0.032490004760333285 0.0 32-33 7.301124665243434E-5 0.0 0.0 0.06180402029128568 0.0 34-35 7.301124665243434E-5 0.0 0.0 0.09327186759848487 0.0 36-37 7.301124665243434E-5 0.0 0.0 0.1126928592080324 0.0 38-39 7.301124665243434E-5 0.0 0.0 0.1279887153817174 0.0 40-41 7.301124665243434E-5 0.0 0.0 0.14007207670269528 0.0 42-43 7.301124665243434E-5 0.0 0.0 0.1569011690560814 0.0 44-45 7.301124665243434E-5 0.0 0.0 0.1702622271934769 0.0 46-47 7.301124665243434E-5 0.0 0.0 0.1934432980056248 0.0 48-49 7.301124665243434E-5 0.0 0.0 0.21527366075470267 0.0 50-51 7.301124665243434E-5 0.0 0.0 0.2317376968748266 0.0 52-53 7.301124665243434E-5 0.0 0.0 0.25882486938287974 0.0 54-55 7.301124665243434E-5 0.0 0.0 0.32705387937957964 0.0 56-57 7.301124665243434E-5 0.0 0.0 0.5062599842879798 0.0 58-59 7.301124665243434E-5 0.0 0.0 0.6528300619427416 0.0 60-61 7.301124665243434E-5 0.0 0.0 0.7473066151109917 0.0 62-63 7.301124665243434E-5 0.0 0.0 0.9049013910102712 0.0 64-65 7.301124665243434E-5 0.0 0.0 1.1226574341511566 0.0 66-67 7.301124665243434E-5 0.0 0.0 1.6953941585161778 0.0 68-69 7.301124665243434E-5 0.0 0.0 2.288501020697228 0.0 70-71 7.301124665243434E-5 0.0 0.0 2.58773761510223 0.0 72-73 7.301124665243434E-5 0.0 0.0 2.8541921597602897 0.0 74-75 7.301124665243434E-5 0.0 0.0 3.194242041044002 0.0 76-77 7.301124665243434E-5 0.0 0.0 3.4872726794835476 0.0 78-79 7.301124665243434E-5 0.0 0.0 3.7212372193812735 0.0 80-81 7.301124665243434E-5 0.0 0.0 3.9773971782613393 0.0 82-83 7.301124665243434E-5 0.0 0.0 4.29758800045559 0.0 84-85 7.301124665243434E-5 0.0 0.0 5.140320314941313 0.0 86-87 7.301124665243434E-5 0.0 0.0 6.396843869829709 0.0 88-89 7.301124665243434E-5 0.0 0.0 7.740214302611174 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCG 95 7.2759576E-12 50.03008 1 TCTAACG 40 5.3842145E-4 47.507744 2 TATAACG 200 0.0 40.38158 2 TACCTGG 1080 0.0 39.589783 2 GAGTACG 2255 0.0 38.14932 1 AGTACGC 2285 0.0 37.21613 2 TAACGCC 65 1.3307556E-4 36.527073 4 TACGCAG 2390 0.0 36.16028 4 GTACGCA 2405 0.0 35.75427 3 CGCAGTG 2490 0.0 35.089462 6 ATAACGC 245 0.0 34.90365 3 ACGCAGT 2510 0.0 34.620678 5 ACCTGGG 1230 0.0 33.216793 3 GTACCGG 90 2.4033985E-5 31.685715 1 GTACCTG 1370 0.0 31.22315 1 GTAGGCA 1120 0.0 30.116514 3 GTATAAC 335 0.0 29.794031 1 CTAACGC 65 0.0058398037 29.235537 3 TAACGCA 295 0.0 28.97402 4 AGTAGGC 1195 0.0 28.623913 2 >>END_MODULE