##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003793_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 389935 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05252926769846 31.0 31.0 34.0 31.0 34.0 2 32.22275763909369 33.0 31.0 34.0 30.0 34.0 3 32.284314052342054 34.0 31.0 34.0 30.0 34.0 4 35.93049354379576 37.0 35.0 37.0 35.0 37.0 5 35.83135650813597 37.0 35.0 37.0 35.0 37.0 6 35.95534640388783 37.0 35.0 37.0 35.0 37.0 7 35.93058586687525 37.0 35.0 37.0 35.0 37.0 8 35.941092746226936 37.0 35.0 37.0 35.0 37.0 9 37.59618910844115 39.0 37.0 39.0 35.0 39.0 10-11 37.51089668790952 39.0 37.0 39.0 35.0 39.0 12-13 37.49712131509098 39.0 37.0 39.0 35.0 39.0 14-15 38.63714721684383 40.0 38.0 41.0 34.0 41.0 16-17 38.693736135509766 40.0 38.0 41.0 34.5 41.0 18-19 38.74837601138651 40.0 38.0 41.0 34.5 41.0 20-21 38.713727929013814 40.0 38.0 41.0 34.5 41.0 22-23 38.622926898072755 40.0 38.0 41.0 34.0 41.0 24-25 38.481923910395324 40.0 38.0 41.0 34.0 41.0 26-27 38.23194378550271 40.0 37.5 41.0 34.0 41.0 28-29 38.0828471411902 40.0 37.5 41.0 34.0 41.0 30-31 37.914366496980264 40.0 37.0 41.0 33.0 41.0 32-33 37.61028248297794 40.0 37.0 41.0 33.0 41.0 34-35 37.50623180786541 39.5 36.5 41.0 33.0 41.0 36-37 37.30762562991268 39.5 36.5 41.0 32.0 41.0 38-39 37.07196584046059 39.0 36.0 41.0 31.0 41.0 40-41 37.14229166399528 40.0 36.5 41.0 31.5 41.0 42-43 37.018924949029966 40.0 36.0 41.0 31.0 41.0 44-45 36.68183286958083 40.0 36.0 41.0 30.5 41.0 46-47 36.47014502417069 40.0 36.0 41.0 30.0 41.0 48-49 36.127616141151734 39.0 36.0 41.0 29.5 41.0 50-51 35.79214484465359 39.0 35.0 41.0 28.0 41.0 52-53 35.655411799402465 39.0 35.0 41.0 28.0 41.0 54-55 35.47273391719133 39.0 35.0 41.0 27.5 41.0 56-57 35.1591714003616 38.5 34.5 40.5 26.5 41.0 58-59 34.89093566876531 38.0 34.0 40.0 26.0 41.0 60-61 34.40418146614179 38.0 34.0 40.0 24.5 41.0 62-63 34.19395283829356 37.5 34.0 40.0 24.5 41.0 64-65 33.740229781886725 37.0 33.5 39.5 22.5 41.0 66-67 33.348440124636156 36.5 33.0 39.0 22.0 41.0 68-69 32.95709541333812 36.0 33.0 39.0 21.5 40.5 70-71 32.415398720299535 35.0 32.0 38.5 20.0 40.0 72-73 31.93968481926475 35.0 31.5 37.5 18.5 40.0 74-75 31.543925269596215 35.0 31.0 37.0 17.5 39.5 76-77 30.61244694628592 34.0 30.5 36.0 13.5 39.0 78-79 31.01152115096106 35.0 31.0 36.0 14.0 39.0 80-81 30.70213368894816 35.0 31.0 36.0 9.5 38.5 82-83 30.386168207521767 34.5 30.5 35.5 7.0 37.0 84-85 30.070870786156668 34.0 31.0 35.0 4.5 37.0 86-87 29.868411401900318 34.0 31.0 35.0 2.0 36.0 88-89 29.539237308782234 34.0 30.0 35.0 2.0 36.0 90-91 29.232350263505456 34.0 30.0 35.0 2.0 36.0 92-93 28.979459397079 34.0 29.5 35.0 2.0 35.0 94-95 28.61122366548271 34.0 29.0 35.0 2.0 35.0 96-97 28.196872555682358 34.0 29.0 35.0 2.0 35.0 98-99 27.969019195506945 34.0 29.0 35.0 2.0 35.0 100-101 27.045855078410504 33.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.1014591182844633 1101 2 -0.02069454087769884 1101 3 -0.0030139193932328112 1101 4 -0.08387747633110365 1101 5 0.045325425298642585 1101 6 0.13834216571599 1101 7 -0.04067117353739036 1101 8 0.0016384419379207316 1101 9 0.17849794098530225 1101 10-11 0.14053602354955075 1101 12-13 0.053398965951167554 1101 14-15 0.18856281248419293 1101 16-17 0.1660802024268193 1101 18-19 0.16966094431414547 1101 20-21 -0.10125082076383762 1101 22-23 -0.13492092572043646 1101 24-25 0.06619199939778042 1101 26-27 -0.10942216110748149 1101 28-29 0.06930887808963604 1101 30-31 -0.151224299571453 1101 32-33 -0.3538018287485656 1101 34-35 -0.728062368704208 1101 36-37 -0.696338936014179 1101 38-39 -0.5921743433514521 1101 40-41 -0.6293608800168329 1101 42-43 -0.571550221390396 1101 44-45 -0.5170311815996271 1101 46-47 -0.7266116019957138 1101 48-49 -0.9252426259616158 1101 50-51 -0.7596738160304426 1101 52-53 -0.8896252232503699 1101 54-55 -0.7483414709946175 1101 56-57 -0.9521492868858061 1101 58-59 -0.8324443719394097 1101 60-61 -1.0711865081525005 1101 62-63 -1.1987799475153693 1101 64-65 -1.6548798921390215 1101 66-67 -1.2233358709201312 1101 68-69 -0.9764205316555277 1101 70-71 -1.0234987824821715 1101 72-73 -0.9552816863911247 1101 74-75 -0.7984886484715652 1101 76-77 -1.1559076490006284 1101 78-79 -1.0865351788631479 1101 80-81 -0.9805965487971733 1101 82-83 -0.8859165071177308 1101 84-85 -1.077886330676538 1101 86-87 -0.9835541141833843 1101 88-89 -1.0528949123493199 1101 90-91 -1.3237663280467373 1101 92-93 -1.2636841115722035 1101 94-95 -1.2818946187546878 1101 96-97 -1.4207916200157662 1101 98-99 -1.5670713117288706 1101 100-101 -2.0131331764101965 1102 1 0.12288654385200459 1102 2 0.17016970208822357 1102 3 0.21236773023041877 1102 4 -0.16520201213896257 1102 5 -0.1930927726106333 1102 6 0.12500567789145123 1102 7 0.09190508190629743 1102 8 0.150688151461857 1102 9 0.26801539145540687 1102 10-11 0.3123506446162949 1102 12-13 0.17354564979229536 1102 14-15 0.011639668157457095 1102 16-17 0.09831121633286699 1102 18-19 0.30711351741509674 1102 20-21 -0.03277645538613427 1102 22-23 0.13598415038166678 1102 24-25 0.20611997184855824 1102 26-27 0.029195821244890396 1102 28-29 -0.0897169098759818 1102 30-31 0.3148955886193576 1102 32-33 0.5691190548594705 1102 34-35 0.4703901781093194 1102 36-37 0.5196955180397751 1102 38-39 0.41678090668577994 1102 40-41 0.5729559185250892 1102 42-43 0.7661249185901511 1102 44-45 0.5984144340957229 1102 46-47 0.44303868760410126 1102 48-49 0.08857141678173974 1102 50-51 0.14432888281204015 1102 52-53 0.3397281936769829 1102 54-55 0.2204553534842404 1102 56-57 0.055907809405034925 1102 58-59 0.09697388730719325 1102 60-61 -0.026835238031658548 1102 62-63 -0.35998759189905627 1102 64-65 -0.6421786837936381 1102 66-67 -0.20383060029107014 1102 68-69 -0.07884948988480289 1102 70-71 -0.028340050237133596 1102 72-73 -0.36354732960064595 1102 74-75 -0.3599968457674656 1102 76-77 -0.3950531323825963 1102 78-79 -0.38284705289380483 1102 80-81 -0.40019207292465 1102 82-83 -0.5481011675279923 1102 84-85 -0.646702432601657 1102 86-87 -0.2609521846862606 1102 88-89 -0.6773352092219902 1102 90-91 -0.5892211443414404 1102 92-93 -0.9064379823957864 1102 94-95 -0.8101097901048959 1102 96-97 -0.31199672328154193 1102 98-99 -0.36053099995999105 1102 100-101 -0.2740183616927432 1103 1 0.07761461244947299 1103 2 0.09572585727200789 1103 3 0.024983908200077565 1103 4 0.06964238433522496 1103 5 0.19306780782949318 1103 6 0.2272422911912102 1103 7 0.21039162025252267 1103 8 0.25175548971213857 1103 9 0.4849009224262062 1103 10-11 0.14452969360592505 1103 12-13 0.22183071305307323 1103 14-15 0.13958534183977633 1103 16-17 0.25388476073533894 1103 18-19 0.29224273729877126 1103 20-21 0.2654036729343048 1103 22-23 0.09943680973287172 1103 24-25 0.26056621517769685 1103 26-27 0.06565321765911136 1103 28-29 0.25342034502597954 1103 30-31 0.41935082637195364 1103 32-33 0.3810006882467647 1103 34-35 0.4697155963500137 1103 36-37 0.047743128521275935 1103 38-39 0.10032710804095046 1103 40-41 -0.007439236478809619 1103 42-43 0.13575075057431718 1103 44-45 0.5837235424485456 1103 46-47 0.4081852967064563 1103 48-49 -0.0054952618774919415 1103 50-51 0.2445867229511478 1103 52-53 0.019976439764405995 1103 54-55 -0.20255238171993994 1103 56-57 -0.5347709697518823 1103 58-59 -0.1398492823276385 1103 60-61 -0.5047689191493703 1103 62-63 -0.46114487438391905 1103 64-65 -0.441408161517451 1103 66-67 -0.39902458002363517 1103 68-69 -0.30600365430287013 1103 70-71 -0.39405330801731253 1103 72-73 -0.3730064649367364 1103 74-75 -0.3419180546179774 1103 76-77 -0.37703430405615634 1103 78-79 -0.10784255568207257 1103 80-81 0.04399752952183178 1103 82-83 -0.008090973848073446 1103 84-85 -0.6263300634705153 1103 86-87 0.06665469904582366 1103 88-89 -0.1924158592189933 1103 90-91 0.3108058389289319 1103 92-93 0.51769405574057 1103 94-95 0.433534150651834 1103 96-97 0.24613723366515217 1103 98-99 0.4047295385061602 1103 100-101 0.24023779948732482 1104 1 -0.1185356425243107 1104 2 0.07543568992301886 1104 3 0.019740785309892317 1104 4 0.0208382472957922 1104 5 -0.003154270745525878 1104 6 0.06018059471774251 1104 7 0.006412592744084122 1104 8 -0.01797804472727904 1104 9 0.10402036507505841 1104 10-11 0.11671600402753057 1104 12-13 0.15494498440263982 1104 14-15 0.139962415746254 1104 16-17 -0.00927691419104093 1104 18-19 0.07691558079513783 1104 20-21 -0.04435356329351947 1104 22-23 0.012853458443636612 1104 24-25 -0.07625056281555942 1104 26-27 -0.11559364005832862 1104 28-29 -0.2508890710481424 1104 30-31 -0.3317085717462689 1104 32-33 -0.11463602797818595 1104 34-35 -0.014285696474814813 1104 36-37 0.15495780926536895 1104 38-39 -0.39144908961760905 1104 40-41 -0.2757183429932084 1104 42-43 -0.28651815698870564 1104 44-45 0.10369050359197018 1104 46-47 0.40877784141665074 1104 48-49 0.14423899079793756 1104 50-51 0.13397837704868465 1104 52-53 -0.1219110844760607 1104 54-55 -0.3464868785956128 1104 56-57 -0.027534741927425443 1104 58-59 -0.2140789742043907 1104 60-61 -0.2769444560307832 1104 62-63 -0.18268477239440273 1104 64-65 -0.18307662377414857 1104 66-67 -0.07019817766781955 1104 68-69 -0.19746023693592463 1104 70-71 -0.514393751887166 1104 72-73 -0.23304022202931662 1104 74-75 -0.37434641057574325 1104 76-77 0.010884966216050174 1104 78-79 -0.1428026935833877 1104 80-81 0.16396089948520398 1104 82-83 -0.07066728642438491 1104 84-85 0.10772509705523348 1104 86-87 0.03677608093191509 1104 88-89 -0.21949335688472615 1104 90-91 -0.5042663437903094 1104 92-93 -0.39617149047791855 1104 94-95 -0.581961791314697 1104 96-97 -0.17191203732529559 1104 98-99 0.4445736812914767 1104 100-101 0.26183874755886194 1105 1 -0.11243063641930462 1105 2 -0.1657120512247232 1105 3 -0.19698693141782542 1105 4 -0.202604976147434 1105 5 -0.15333742092867908 1105 6 -0.09000255546540359 1105 7 -0.057079470747979144 1105 8 -0.17609770284693127 1105 9 0.09425235530704867 1105 10-11 -0.21570157839006043 1105 12-13 0.21416354362119705 1105 14-15 -0.2702939945101548 1105 16-17 0.06673041181628037 1105 18-19 -0.18437868049912254 1105 20-21 0.1546696357296824 1105 22-23 0.03269472828490194 1105 24-25 -0.2951150316800266 1105 26-27 -0.21174748621218242 1105 28-29 -0.37604169620076533 1105 30-31 -0.339950329988028 1105 32-33 -0.03466044800260448 1105 34-35 -0.18400486619398038 1105 36-37 -0.08008492577736348 1105 38-39 -0.07124151941003021 1105 40-41 -0.07669514397001365 1105 42-43 -0.20776357823412184 1105 44-45 0.07530222520369279 1105 46-47 -0.5133833307445173 1105 48-49 -0.006249409690461505 1105 50-51 0.012183505253808846 1105 52-53 0.023998561433586474 1105 54-55 -0.3470973792061116 1105 56-57 -0.5934688078614911 1105 58-59 -0.7314782416036536 1105 60-61 -0.7894597185460484 1105 62-63 -1.1017934415030695 1105 64-65 -0.6839923746899004 1105 66-67 -0.6913825488521894 1105 68-69 -0.7292062686819492 1105 70-71 -0.5610970485904616 1105 72-73 -0.8609400999291914 1105 74-75 -1.0175088037381386 1105 76-77 -0.7443042889732006 1105 78-79 -0.7609345617152563 1105 80-81 -1.0008742653499603 1105 82-83 -1.4439884097455078 1105 84-85 -1.2249977356675998 1105 86-87 -0.7739687298128999 1105 88-89 -1.1080769954683625 1105 90-91 -0.8577461972701599 1105 92-93 -0.9270017713081984 1105 94-95 -1.0831827925356983 1105 96-97 -1.9411428065560656 1105 98-99 -1.4849134981956986 1105 100-101 -1.3776606419405262 1106 1 -0.10815713214580214 1106 2 -0.14861803413070618 1106 3 -0.04741428184517105 1106 4 -0.07012634366879666 1106 5 0.11589334830209452 1106 6 -0.26521623067907996 1106 7 0.024117110448599988 1106 8 -0.3726788994281307 1106 9 -0.13651687546217772 1106 10-11 -0.1601460228345033 1106 12-13 -0.3520757726181216 1106 14-15 -0.39422561844178006 1106 16-17 -0.36275676767089493 1106 18-19 -0.43224192836236597 1106 20-21 -0.27695429589424947 1106 22-23 -0.3946556990655239 1106 24-25 -0.28015776672275905 1106 26-27 -0.14764492210961322 1106 28-29 -0.45082802098709607 1106 30-31 -0.3314033214410159 1106 32-33 -0.37654078988294515 1106 34-35 -0.13485956704867874 1106 36-37 -0.3343584300508766 1106 38-39 -0.5370534852220032 1106 40-41 -0.13438745166232025 1106 42-43 -0.19921656968711687 1106 44-45 0.015473165374636721 1106 46-47 -0.1715029888641766 1106 48-49 -0.09385624729730324 1106 50-51 -0.3318335887632813 1106 52-53 -0.29651425907923823 1106 54-55 -0.06718284929158358 1106 56-57 -0.20671667110935488 1106 58-59 -0.24857225869767063 1106 60-61 -0.6398870689734011 1106 62-63 -0.20435754406717166 1106 64-65 -0.27587271657024104 1106 66-67 -0.3922372497068949 1106 68-69 -0.7334797729554552 1106 70-71 -0.7128064502998654 1106 72-73 -0.22632471531380816 1106 74-75 -4.147866441215342E-4 1106 76-77 0.1360375913686802 1106 78-79 0.11940731862662446 1106 80-81 -0.07138708586278142 1106 82-83 -0.4033901191472147 1106 84-85 -0.05192081259067649 1106 86-87 0.09568938984521935 1106 88-89 0.02653838914702078 1106 90-91 0.14866405914009562 1106 92-93 0.04735720305077251 1106 94-95 0.053569344216438 1106 96-97 0.39432727891401953 1106 98-99 -0.01269127597347719 1106 100-101 -0.9139854282653133 1107 1 -0.08636649154327714 1107 2 -0.10245572559216498 1107 3 -0.21111911386683602 1107 4 -0.22748438716545394 1107 5 -0.36455854304088575 1107 6 -0.24308709468855483 1107 7 -0.23715901716416 1107 8 -0.07966882919033935 1107 9 0.01716003068997196 1107 10-11 -0.27891559209911776 1107 12-13 -0.1532097321679231 1107 14-15 -0.15268800314179032 1107 16-17 -0.263619930910302 1107 18-19 0.10501372027942324 1107 20-21 -0.021369121992243834 1107 22-23 -0.1973340438230764 1107 24-25 -0.23551868743021487 1107 26-27 -0.4845959249022016 1107 28-29 -0.8446674049254881 1107 30-31 -0.887741647581322 1107 32-33 -0.6662716860494768 1107 34-35 -0.7063667177637569 1107 36-37 -0.47445320875950614 1107 38-39 -0.19445384064216142 1107 40-41 0.18773322194350328 1107 42-43 0.4635997119410433 1107 44-45 0.21099656386832777 1107 46-47 -0.24935692810426247 1107 48-49 -0.6629727743346123 1107 50-51 -0.8296545247030238 1107 52-53 -0.6600371498298543 1107 54-55 -0.5577261343893269 1107 56-57 -0.6101608616822531 1107 58-59 -0.5175914683109397 1107 60-61 -0.5872933480628504 1107 62-63 -0.6293808156251046 1107 64-65 -0.4163483025903858 1107 66-67 -0.6959733925519416 1107 68-69 -0.8403173797930634 1107 70-71 -1.0093707221116617 1107 72-73 -0.666897618758 1107 74-75 -0.36152589775523225 1107 76-77 -0.9071417265631148 1107 78-79 -0.8879338001006296 1107 80-81 -0.6581476849996157 1107 82-83 -0.5168918539360803 1107 84-85 -0.7449478075978675 1107 86-87 -0.901814206668277 1107 88-89 -0.8871029749893928 1107 90-91 -0.7674313965593242 1107 92-93 -0.6994482467249696 1107 94-95 -0.7644047608863787 1107 96-97 -0.9800486202143546 1107 98-99 -0.9020879079443667 1107 100-101 -1.0285449611416766 1108 1 -0.13494177160649556 1108 2 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-0.3182361019703137 2316 50-51 -0.1533556244546972 2316 52-53 0.05395485197619365 2316 54-55 0.08937774537976395 2316 56-57 0.33666566136092513 2316 58-59 0.4844242055691481 2316 60-61 0.3364416150100027 2316 62-63 0.2242248218116103 2316 64-65 0.6939413094327094 2316 66-67 0.6194070054227083 2316 68-69 0.740301512715078 2316 70-71 0.8718980236554188 2316 72-73 0.8219436106418065 2316 74-75 0.5537835484988385 2316 76-77 0.7946514169206154 2316 78-79 0.9843047266836429 2316 80-81 0.8702733167096746 2316 82-83 0.7331323341504792 2316 84-85 0.6398927122847375 2316 86-87 0.5136645561917206 2316 88-89 0.687323767359107 2316 90-91 0.5678154831775117 2316 92-93 0.5492294712096886 2316 94-95 0.5171123159027324 2316 96-97 0.4204415916732813 2316 98-99 0.46522431187696256 2316 100-101 0.4328992288799327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 9.0 6 18.0 7 50.0 8 104.0 9 199.0 10 355.0 11 640.0 12 1134.0 13 1814.0 14 1864.0 15 2434.0 16 3034.0 17 4515.0 18 3985.0 19 2585.0 20 2266.0 21 2175.0 22 2255.0 23 2396.0 24 2718.0 25 3028.0 26 3695.0 27 4302.0 28 5358.0 29 6793.0 30 8356.0 31 10428.0 32 13555.0 33 17222.0 34 24163.0 35 34455.0 36 54597.0 37 78380.0 38 73196.0 39 17854.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.00642029037572 19.65322220568537 13.43667725595455 17.903680247984358 2 15.389882669744182 25.59902545361287 37.50362249150478 21.507469385138165 3 25.9473774702898 27.18813593683038 28.231810121612295 18.632676471267523 4 12.845474245707619 17.443163604190442 41.343044353546105 28.368317796555836 5 11.898136868965342 40.80090271455499 33.70125790195802 13.599702514521649 6 26.80959647120674 41.20379037531897 17.01001448959442 14.976598663879878 7 27.955941374844524 34.06388244194545 21.534871196481465 16.445304986728555 8 24.603844230448665 31.497813738187137 20.060779360662675 23.837562670701526 9 23.63855514380602 23.628297023862952 22.090348391398567 30.64279944093246 10-11 25.23959121392027 26.197314937104903 27.959916396322466 20.603177452652368 12-13 30.319066407306867 24.170854335792296 25.923841055356732 19.586238201544106 14-15 22.866499883953082 27.68315216764099 29.4765578817655 19.973790066640422 16-17 19.65481426391578 29.546591098516416 29.50850782822778 21.290086809340018 18-19 19.210252990883095 33.00101298934438 29.37784502545296 18.410888994319567 20-21 22.378820502889944 27.1219957603046 28.257454413015072 22.241729323790384 22-23 20.100273122443486 29.12947029633144 28.239578391270342 22.530678189954738 24-25 24.16838703886545 28.539371946606483 28.44089399515304 18.851347019375023 26-27 23.745179934163705 25.78502794900829 32.113724700665415 18.356067416162595 28-29 20.287806873662248 31.818164330261016 29.915396936745843 17.978631859330896 30-31 22.256876004293535 28.31142749786157 28.519050959440968 20.91264553840393 32-33 21.90421354726359 29.598316530267322 28.938290729375314 19.559179193093772 34-35 23.931060572058758 26.667478825105956 30.122272590293854 19.279188012541436 36-37 23.643725527163966 26.297853893401363 31.475289569842413 18.58313100959226 38-39 21.51206655854154 28.59841097127841 31.539987476647024 18.349534993533023 40-41 20.389951784981537 29.737382027082475 29.46206914238818 20.410597045547803 42-43 19.889242718247377 31.05282260495073 26.844148498889993 22.2137861779119 44-45 22.86114053694959 26.794769525148194 27.44575742108247 22.898332516819753 46-47 24.373351388712567 29.701355307550557 27.27157316467968 18.653720139057196 48-49 24.337570012925465 28.89841201452576 27.791156316037835 18.972861656510943 50-51 20.171494957991815 29.59961428380915 31.54986612775823 18.679024630440804 52-53 22.440466802332214 32.033354747460756 26.015856712559575 19.510321737647445 54-55 20.29941271510271 30.269663785807708 27.13307516733773 22.29784833175185 56-57 23.403510436797646 27.632731418370867 27.97320813707298 20.99055000775851 58-59 26.522512448497267 27.405929370246458 26.035344371022283 20.036213810233992 60-61 22.191503276355103 29.402305630714388 30.355077388661634 18.05111370426888 62-63 20.703579837191526 28.675501274666203 29.74498981805871 20.87592907008356 64-65 20.049595814647194 33.10161237739027 27.65505751522915 19.193734292733392 66-67 22.262035344302255 27.639372654671412 26.623047564065555 23.475544436960774 68-69 21.318084507331314 30.031900933939284 26.231110529617073 22.418904029112326 70-71 24.467256900490067 29.164887115536796 26.381348508575837 19.986507475397307 72-73 23.704041843032208 26.79085023471951 29.67762451802679 19.82748340422149 74-75 21.10081155487965 32.37294851261956 27.718016415745883 18.80822351675491 76-77 22.41376657348755 28.720539584027904 26.53758366886364 22.328110173620907 78-79 22.135440041648607 29.997897053053247 26.761153953668487 21.105508951629655 80-81 23.96358805622591 28.531750814896125 27.164187142721584 20.340473986156383 82-83 22.6492805732333 27.851259910470766 29.230743585041875 20.268715931254064 84-85 20.93009836469181 29.495430549090663 28.680148694689894 20.894322391527634 86-87 20.882331065224584 29.695895603733273 26.620099051292772 22.80167427974937 88-89 20.362446417145488 30.20245759853924 25.692457816525128 23.742638167790147 90-91 22.27827195289429 25.58128799545561 27.113864871137622 25.026575180512474 92-93 23.164748815822207 28.520284253961574 27.176335421726293 21.13863150848992 94-95 22.571532257961103 28.593539816086473 27.769891372785878 21.065036553166554 96-97 18.68702478035218 29.38811522870176 30.869704054138968 21.055155936807086 98-99 21.223387406011902 32.22238324187275 24.9780379171353 21.576191434980053 100-101 19.007476947148373 31.001244020366027 25.3496723224706 24.641606710015004 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 86.0 1 64.0 2 110.5 3 160.0 4 114.0 5 103.5 6 119.0 7 174.5 8 286.5 9 361.0 10 378.5 11 387.0 12 418.5 13 463.0 14 523.5 15 566.0 16 584.5 17 632.0 18 688.5 19 745.5 20 825.0 21 863.5 22 934.5 23 1072.5 24 1207.0 25 1387.0 26 1697.5 27 2257.5 28 3136.0 29 4245.5 30 5440.0 31 6687.0 32 7835.5 33 8995.0 34 9791.5 35 10182.0 36 10551.0 37 10836.5 38 11159.5 39 11236.0 40 11219.5 41 11299.0 42 11419.5 43 11877.0 44 12119.0 45 12291.0 46 13490.5 47 21639.5 48 39726.5 49 38552.0 50 21610.5 51 14095.5 52 10083.5 53 8044.5 54 6839.5 55 5889.5 56 5109.5 57 4218.0 58 3334.5 59 2628.0 60 2067.5 61 1563.5 62 1140.5 63 850.0 64 578.5 65 367.5 66 243.0 67 155.5 68 93.0 69 52.5 70 29.5 71 15.0 72 12.0 73 6.5 74 5.0 75 3.0 76 1.5 77 0.5 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0597535486683678 2 0.0025645299857668586 3 0.0651390616384782 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.2822649928834293E-4 14-15 0.0016669444907484582 16-17 0.0 18-19 0.0 20-21 0.012437970430969263 22-23 0.0 24-25 0.0 26-27 0.007308910459435547 28-29 0.014233141421006066 30-31 0.011925064433815894 32-33 0.006667777962993833 34-35 0.0070524574608588604 36-37 0.022952543372613383 38-39 0.06744713862566838 40-41 0.003846794978650288 42-43 0.01884929539538641 44-45 0.016925897906061266 46-47 0.006026645466552118 48-49 0.001795170990036801 50-51 0.0028209829843435443 52-53 0.0014104914921717721 54-55 0.0012822649928834293 56-57 0.010386346442355778 58-59 0.007308910459435547 60-61 0.005129059971533717 62-63 0.007437136958723891 64-65 0.09899085745060075 66-67 0.05064946721889546 68-69 0.03449292830856425 70-71 0.022952543372613383 72-73 3.846794978650288E-4 74-75 0.0011540384935950863 76-77 0.0012822649928834293 78-79 0.0015387179914601152 80-81 0.002051623988613487 82-83 0.0016669444907484582 84-85 0.00217985048790183 86-87 0.00923230794876069 88-89 3.846794978650288E-4 90-91 8.975854950184005E-4 92-93 5.129059971533717E-4 94-95 0.006026645466552118 96-97 0.01269442342954595 98-99 0.015002500416736123 100-101 0.01795170990036801 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 389935.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.32143210691756 #Duplication Level Percentage of deduplicated Percentage of total 1 88.358454511019 55.06625423880636 2 7.562381911140687 9.425969416834716 3 1.6660370680300203 3.1148944806852272 4 0.7264834142225906 1.811019471050994 5 0.4127276305992981 1.2860888504521555 6 0.2728333172730158 1.0202017835361212 7 0.18078388950972696 0.7886697627273457 8 0.12776708821116656 0.6370102330760612 9 0.10332983907296744 0.5795697195364194 >10 0.5157621341166329 5.685283286542521 >50 0.035670467019592894 1.5456628727671053 >100 0.026018458296575894 3.29558339419628 >500 0.0037768729785352105 1.6970907582683645 >1k 0.007553745957070421 9.703938279363445 >5k 0.0 0.0 >10k+ 4.196525531705789E-4 4.342763452156911 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16605 4.258402041365868 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4909 1.2589277700129509 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3374 0.8652724171977381 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2926 0.7503814738353828 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2798 0.717555490017567 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2797 0.7172990370189903 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2419 0.620359803557003 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2364 0.6062548886352854 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2340 0.6001000166694449 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1637 0.4198135586700348 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1582 0.405708643748317 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1498 0.38416659186787544 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1446 0.3708310359418877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1267 0.32492594919666096 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1247 0.3197968892251273 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1229 0.3151807352507469 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1151 0.2951774013617654 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1110 0.2846628284201213 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1010 0.2590175285624527 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 966 0.24773359662507854 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 937 0.24029645966635463 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 842 0.2159334248015695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 690 0.17695256901791326 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 660 0.16925897906061266 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 628 0.16105248310615872 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 627 0.16079603010758203 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 612 0.15694923512893175 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 527 0.13515073024991345 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 467 0.11976355033531229 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 415 0.10642799440932463 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 403 0.10335055842640439 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.846794978650288E-4 0.0 12-13 0.0 0.0 0.0 0.0010258119943067434 0.0 14-15 0.0 0.0 0.0 0.001666944490748458 0.0 16-17 0.0 0.0 0.0 0.0021798504879018297 0.0 18-19 0.0 0.0 0.0 0.002564529985766858 0.0 20-21 0.0 0.0 0.0 0.003718568479361945 0.0 22-23 0.0 0.0 0.0 0.005770192467975431 0.0 24-25 0.0 0.0 0.0 0.008719401951607319 0.0 26-27 0.0 0.0 0.0 0.011540384935950863 0.0 28-29 0.0 0.0 0.0 0.017310577403926296 0.0 30-31 0.0 0.0 0.0 0.026927564850552015 0.0 32-33 0.0 0.0 0.0 0.045007501250208366 0.0 34-35 0.0 0.0 0.0 0.0597535486683678 0.0 36-37 0.0 0.0 0.0 0.07424314308795055 0.0 38-39 0.0 0.0 0.0 0.08668111351891983 0.0 40-41 0.0 0.0 0.0 0.11091592188441664 0.0 42-43 0.0 0.0 0.0 0.14284432020721405 0.0 44-45 0.0 0.0 0.0 0.22054957877594983 0.0 46-47 0.0 0.0 0.0 0.3930142203187711 0.0 48-49 0.0 0.0 0.0 0.47815661584623076 0.0 50-51 0.0 0.0 0.0 0.5763781143011015 0.0 52-53 0.0 0.0 0.0 0.6704963647787452 0.0 54-55 0.0 0.0 0.0 0.7674355982407324 0.0 56-57 0.0 0.0 0.0 0.8683498531806584 0.0 58-59 0.0 0.0 0.0 1.0576121661302524 0.0 60-61 0.0 0.0 0.0 1.2386679831253926 0.0 62-63 0.0 0.0 0.0 1.4602433738956493 0.0 64-65 0.0 0.0 0.0 1.5788528857373665 0.0 66-67 0.0 0.0 0.0 2.0938105068793518 0.0 68-69 0.0 0.0 0.0 2.6390295818533858 0.0 70-71 0.0 0.0 0.0 2.811109543898342 0.0 72-73 0.0 0.0 0.0 3.5376408888660933 0.0 74-75 0.0 0.0 0.0 3.8098657468552455 0.0 76-77 0.0 0.0 0.0 3.962327054509085 0.0 78-79 0.0 0.0 0.0 4.183902445279341 0.0 80-81 0.0 0.0 0.0 4.380858348186236 0.0 82-83 0.0 0.0 0.0 4.768358829035609 0.0 84-85 0.0 0.0 0.0 5.731852744688217 0.0 86-87 0.0 0.0 0.0 7.591136984369189 0.0 88-89 0.0 0.0 0.0 9.350019875107389 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 60 3.2923708E-10 63.320335 2 ATAACGC 65 6.693881E-10 58.449543 3 TACCTGG 630 0.0 48.24407 2 CAAACGC 50 2.8291024E-5 47.48416 4 GTATAAC 105 2.0008883E-11 45.252026 1 ACCTGGG 655 0.0 43.50252 3 TACCCCG 45 9.633036E-4 42.208145 5 GTACACG 70 4.2937554E-6 40.72682 1 GTACCTG 750 0.0 39.91229 1 CCTGGGG 420 0.0 36.17841 4 ACACGGG 80 1.0772979E-5 35.617687 3 TACACGG 80 1.0772979E-5 35.617687 2 CGAGATA 30 0.0039281496 31.702862 62-63 GTACTAT 105 1.8928549E-6 31.676418 1 TTGTACG 60 0.0039517465 31.660168 9 CTAGGTA 60 0.0039517465 31.660168 2 CACGGGG 75 3.0807653E-4 31.65611 4 TAACGCA 120 1.5058868E-7 31.656107 4 TACGCAG 190 1.2732926E-11 29.989998 4 ACTTAGA 160 1.891749E-9 29.696642 1 >>END_MODULE