##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003792_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 588185 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92057430910343 31.0 31.0 34.0 30.0 34.0 2 32.05499970247456 33.0 31.0 34.0 30.0 34.0 3 32.18645494189753 33.0 31.0 34.0 30.0 34.0 4 35.687067844300685 37.0 35.0 37.0 35.0 37.0 5 35.645160961262185 37.0 35.0 37.0 33.0 37.0 6 35.854569565697865 37.0 35.0 37.0 35.0 37.0 7 35.86110492447104 37.0 35.0 37.0 35.0 37.0 8 35.88784141044059 37.0 35.0 37.0 35.0 37.0 9 37.49790457084081 39.0 37.0 39.0 35.0 39.0 10-11 37.37921997330772 39.0 37.0 39.0 34.5 39.0 12-13 37.41386213521256 39.0 37.0 39.0 34.0 39.0 14-15 38.2647406853286 40.0 37.5 41.0 33.5 41.0 16-17 38.50961177180649 40.0 38.0 41.0 34.0 41.0 18-19 38.63732414121408 40.0 38.0 41.0 34.0 41.0 20-21 38.586551850183184 40.0 38.0 41.0 34.0 41.0 22-23 38.482177376165666 40.0 38.0 41.0 34.0 41.0 24-25 38.23335090150208 40.0 37.5 41.0 33.5 41.0 26-27 37.796450096483255 39.5 36.5 41.0 33.0 41.0 28-29 37.735193859074954 39.0 37.0 41.0 33.0 41.0 30-31 37.75607334427093 40.0 37.0 41.0 33.0 41.0 32-33 37.480680398174044 39.0 36.0 41.0 32.5 41.0 34-35 37.202332599437256 39.0 36.0 40.0 31.5 41.0 36-37 36.966289517753765 38.5 36.0 40.5 31.0 41.0 38-39 36.79209857442811 39.0 35.0 40.0 31.0 41.0 40-41 37.12117871077977 39.0 36.0 41.0 31.5 41.0 42-43 37.01766876067904 39.5 36.0 41.0 31.0 41.0 44-45 36.7131183216165 39.0 36.0 41.0 30.5 41.0 46-47 36.539410219573774 39.0 36.0 41.0 30.0 41.0 48-49 36.10292510009606 39.0 35.0 41.0 29.0 41.0 50-51 35.5487848211022 38.5 35.0 40.0 28.0 41.0 52-53 35.67940443907954 39.0 35.0 40.0 28.0 41.0 54-55 35.57573212509669 39.0 35.0 40.0 28.0 41.0 56-57 35.01986449841461 38.5 34.5 40.0 26.5 41.0 58-59 34.738532094494076 38.0 34.0 40.0 26.0 41.0 60-61 33.883332624939435 37.0 33.0 39.5 22.5 41.0 62-63 33.95909280243461 37.0 33.0 40.0 24.5 41.0 64-65 33.5113671718932 36.5 32.5 39.0 23.0 40.5 66-67 33.00727832229656 36.0 32.0 39.0 22.0 40.0 68-69 32.42718787456327 35.5 32.0 37.5 21.5 39.5 70-71 31.657061978799188 35.0 31.5 37.0 19.5 39.0 72-73 31.00256891964263 34.5 30.0 36.5 17.0 39.0 74-75 30.67933473311968 34.0 30.5 36.0 17.0 38.5 76-77 29.915167846850906 33.5 29.5 35.0 14.0 37.0 78-79 30.362462490542942 34.0 30.5 35.0 12.5 37.0 80-81 29.828188410109064 34.0 29.5 35.0 7.0 36.5 82-83 29.479885580217108 34.0 29.5 35.0 7.0 36.0 84-85 29.23944847284443 34.0 29.0 35.0 4.0 36.0 86-87 29.331683908974217 34.0 29.0 35.0 2.0 35.5 88-89 28.976628101702694 34.0 29.0 35.0 2.0 35.0 90-91 28.55017468993599 34.0 29.0 35.0 2.0 35.0 92-93 28.340536565876384 34.0 29.0 35.0 2.0 35.0 94-95 27.78440881695385 33.0 27.0 35.0 2.0 35.0 96-97 27.095279546401215 33.0 25.0 35.0 2.0 35.0 98-99 27.11696830078972 34.0 27.0 35.0 2.0 35.0 100-101 26.05846969915928 33.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.12130926365134798 1101 2 0.09627788997180176 1101 3 0.194639637733367 1101 4 0.08484841511856445 1101 5 0.1295236171509373 1101 6 0.023145884434718766 1101 7 0.13724358049177 1101 8 0.09621621027159222 1101 9 0.17256433718493014 1101 10-11 0.2097372131026347 1101 12-13 0.3195969869905326 1101 14-15 0.32010935838433596 1101 16-17 0.26907270464047883 1101 18-19 0.2560058996658796 1101 20-21 0.07762187345203841 1101 22-23 0.008829939746178184 1101 24-25 0.0376600650495007 1101 26-27 0.5080569501627465 1101 28-29 0.5462927214869708 1101 30-31 0.4757671417102145 1101 32-33 0.3683234256399004 1101 34-35 0.39171231157997255 1101 36-37 0.4351386184642436 1101 38-39 0.4418632511994005 1101 40-41 0.3378255266441883 1101 42-43 0.3566831779937516 1101 44-45 0.3577512001853833 1101 46-47 0.4414974576831199 1101 48-49 0.15398883261941876 1101 50-51 0.2710296986999623 1101 52-53 -0.08677568889019227 1101 54-55 -0.01603484313559278 1101 56-57 0.008371553768355966 1101 58-59 0.1312556855268312 1101 60-61 0.08558248874343377 1101 62-63 0.1466849155383656 1101 64-65 0.2016933706182158 1101 66-67 -0.04082155918540309 1101 68-69 0.1948821968141914 1101 70-71 0.170063427140839 1101 72-73 0.3780269618078087 1101 74-75 0.3475688076547492 1101 76-77 0.0911912585258996 1101 78-79 0.04901927320453581 1101 80-81 0.26280691945968826 1101 82-83 0.5339052055031566 1101 84-85 0.7195010297438422 1101 86-87 0.14672432809537028 1101 88-89 0.15007928388272873 1101 90-91 0.3407477308350657 1101 92-93 0.5945282351519978 1101 94-95 0.3353540369374315 1101 96-97 0.15672828680115103 1101 98-99 0.0016714820826031485 1101 100-101 0.010555549832570676 1102 1 0.01933964697585111 1102 2 0.1025488668310146 1102 3 -0.11829093990309048 1102 4 -0.03142962763006807 1102 5 -0.07454810162666092 1102 6 0.05754793354265075 1102 7 0.04737429944601956 1102 8 0.09259494194444073 1102 9 0.05765531051120121 1102 10-11 -0.008466284512621769 1102 12-13 0.03698349114175414 1102 14-15 0.020096109841666987 1102 16-17 -0.010339060065405192 1102 18-19 0.0962726369914364 1102 20-21 -0.09681727157534681 1102 22-23 -0.12937444110526997 1102 24-25 -0.1373752643975905 1102 26-27 -0.07485684598635345 1102 28-29 0.11809982270583674 1102 30-31 0.053580248935084285 1102 32-33 0.1823138866891867 1102 34-35 0.04621239990359527 1102 36-37 -0.08133373624340123 1102 38-39 0.11581661632923357 1102 40-41 -0.01224513225000834 1102 42-43 0.1477978220495757 1102 44-45 0.28013682110040605 1102 46-47 0.19333017275112496 1102 48-49 0.4060644376362035 1102 50-51 0.46547414314440516 1102 52-53 0.2808492890289074 1102 54-55 0.20373639869447402 1102 56-57 0.40978031547123805 1102 58-59 0.40488225327104743 1102 60-61 0.4583081555867423 1102 62-63 0.34351409766166086 1102 64-65 0.14196452412465987 1102 66-67 6.68460245790925E-4 1102 68-69 0.021988715097180034 1102 70-71 -0.07178871912307372 1102 72-73 0.03705363554036367 1102 74-75 0.16765359832777804 1102 76-77 -0.07702107145113857 1102 78-79 0.0696207570413172 1102 80-81 -0.020866459801929693 1102 82-83 -0.09712288140728376 1102 84-85 -0.3050750168910348 1102 86-87 -0.1281608512015744 1102 88-89 -0.20880165588055988 1102 90-91 -0.20378945340800314 1102 92-93 -0.08490119427743181 1102 94-95 -0.17142480072375932 1102 96-97 0.03455464256868268 1102 98-99 0.14870822470757972 1102 100-101 -0.023625690231018126 1103 1 -0.11589323405534913 1103 2 -0.163418126026734 1103 3 -0.01252135934359444 1103 4 -0.14341684676013955 1103 5 -0.10165288293312358 1103 6 -0.05904718935499176 1103 7 -0.12249667924849206 1103 8 0.08667136039061063 1103 9 0.02564856842042218 1103 10-11 -0.14513004092427906 1103 12-13 -0.07976472329553985 1103 14-15 -0.14051813585660966 1103 16-17 -0.25166286231358015 1103 18-19 -0.251972721758122 1103 20-21 -0.1477527973007895 1103 22-23 -0.25077906356030866 1103 24-25 -0.26600095709891747 1103 26-27 -0.28937750273389184 1103 28-29 -0.13267321378148011 1103 30-31 -0.18161079718033335 1103 32-33 -0.07049632830590014 1103 34-35 -0.3038715730795474 1103 36-37 -0.5454133074658287 1103 38-39 -0.3618198928198808 1103 40-41 -0.36520752933004275 1103 42-43 -0.24671149682949078 1103 44-45 -0.01963611837840773 1103 46-47 0.19102773662515915 1103 48-49 0.4080894900814229 1103 50-51 0.15752975326724084 1103 52-53 0.10909725488191668 1103 54-55 0.2928705371983611 1103 56-57 0.027971531351525414 1103 58-59 0.12430941807636486 1103 60-61 -0.04802771209366341 1103 62-63 -0.1530693842159394 1103 64-65 -0.009903798962149324 1103 66-67 0.30238156973201313 1103 68-69 0.19237505144990763 1103 70-71 -0.13122613045124254 1103 72-73 0.012610524962799019 1103 74-75 0.0036607284525516093 1103 76-77 0.010198664928264378 1103 78-79 0.31031248827926206 1103 80-81 -0.023696379648654897 1103 82-83 0.13103201691568245 1103 84-85 0.2170838466543863 1103 86-87 0.12602415613469375 1103 88-89 0.02128280214507683 1103 90-91 0.257084290028768 1103 92-93 -0.2136102536587572 1103 94-95 -0.3851390743119829 1103 96-97 -0.0042260212119806795 1103 98-99 0.4550474270551881 1103 100-101 0.4237714504434287 1104 1 -0.046319950196348714 1104 2 -0.12371497203786674 1104 3 -0.03385716639368752 1104 4 -0.10909402672303514 1104 5 -0.05174564731160558 1104 6 -0.07667242126593976 1104 7 -0.09540948073271949 1104 8 -0.3747386581622649 1104 9 -0.14893399187642586 1104 10-11 0.048376452582211016 1104 12-13 -0.008057858731532974 1104 14-15 -0.2803140542239575 1104 16-17 0.019672944736512932 1104 18-19 -0.32841056962454473 1104 20-21 -0.15925558023214137 1104 22-23 -0.1455842583655027 1104 24-25 -0.05236459346254918 1104 26-27 -0.19726247490458348 1104 28-29 -0.13666208205606978 1104 30-31 0.009669351242671098 1104 32-33 -0.06780616133001871 1104 34-35 -0.10294392929475293 1104 36-37 -0.12407750041574417 1104 38-39 -0.013675180389448371 1104 40-41 0.235627350076264 1104 42-43 0.3198933269181907 1104 44-45 0.4503082229945008 1104 46-47 0.4168162338422263 1104 48-49 0.45975924889403785 1104 50-51 0.7767319796123218 1104 52-53 0.5713421528410976 1104 54-55 0.8523325038031828 1104 56-57 0.3872479691993931 1104 58-59 0.3201350210448197 1104 60-61 0.2825845328043002 1104 62-63 0.2084333987154139 1104 64-65 0.36903868712319365 1104 66-67 0.24458936193980207 1104 68-69 0.006382472600186873 1104 70-71 -0.1195378187629288 1104 72-73 0.058528892309741565 1104 74-75 0.08009857631897077 1104 76-77 0.21910404526221683 1104 78-79 0.19992287788965157 1104 80-81 -0.015347585585573142 1104 82-83 0.18260901134982177 1104 84-85 0.2170838466543863 1104 86-87 0.43928946225714327 1104 88-89 0.21042936986307126 1104 90-91 0.008011933813552474 1104 92-93 0.17247508956944202 1104 94-95 0.39463829117966753 1104 96-97 0.2148834407546225 1104 98-99 0.3030066107286551 1104 100-101 0.4418605042467725 1105 1 -0.025342015875413182 1105 2 -0.07319181861405966 1105 3 0.02120343652768497 1105 4 0.0022703159683530316 1105 5 -0.0628613626332637 1105 6 -0.014670970939612005 1105 7 -0.03534525199553684 1105 8 -0.1930956456273023 1105 9 -0.19698123107666987 1105 10-11 -0.19717933318386116 1105 12-13 0.13797964256339412 1105 14-15 -0.17271020049972918 1105 16-17 -0.2824352081693178 1105 18-19 -0.17365039144159766 1105 20-21 -0.3261311629525423 1105 22-23 -0.1894462701753099 1105 24-25 -0.1515876329321415 1105 26-27 -0.61439186473158 1105 28-29 -0.5172639674802824 1105 30-31 -0.2436146019325065 1105 32-33 -0.015506358160024547 1105 34-35 -0.5471944213697597 1105 36-37 -0.9475209894864989 1105 38-39 -0.3098577143167276 1105 40-41 -0.1400278866377107 1105 42-43 -0.2661731809032162 1105 44-45 -0.41091108706454804 1105 46-47 -0.48929067592675324 1105 48-49 -0.17185268521970443 1105 50-51 -0.5837777064956171 1105 52-53 -0.636966145097368 1105 54-55 -0.6632429649630041 1105 56-57 -0.9563032583982505 1105 58-59 -0.7911878837516113 1105 60-61 -0.976987620930295 1105 62-63 -0.9640984566663278 1105 64-65 -0.8926934168859191 1105 66-67 -0.8675467615451211 1105 68-69 -1.1895669731679703 1105 70-71 -1.2357555749009173 1105 72-73 -1.2623558067475464 1105 74-75 -0.9920706974819176 1105 76-77 -0.7767987828741134 1105 78-79 -0.41941825958056356 1105 80-81 -0.6891795549215303 1105 82-83 -0.7313918174080776 1105 84-85 -0.5310255493882927 1105 86-87 -0.4558415850856541 1105 88-89 -0.7294753229785336 1105 90-91 -0.6252626958352607 1105 92-93 -0.9588519590164921 1105 94-95 -0.7695179296843087 1105 96-97 -0.7390635187398331 1105 98-99 -0.7456673766327881 1105 100-101 -0.7345095361551763 1106 1 -0.020109466002672605 1106 2 0.002175384104589284 1106 3 -0.058066850267238124 1106 4 -0.07928210195312602 1106 5 -0.05050515111312137 1106 6 -0.027652813422804456 1106 7 0.0448066057018508 1106 8 0.1789628224299733 1106 9 0.174835515926695 1106 10-11 -0.006365444176488211 1106 12-13 -0.18558887113649547 1106 14-15 0.14439582972962484 1106 16-17 0.03237802676932944 1106 18-19 0.06802800580562973 1106 20-21 0.3168148479391206 1106 22-23 -0.11665268573645449 1106 24-25 -0.0056058899811546326 1106 26-27 -0.15410521199942195 1106 28-29 -0.14240437897483105 1106 30-31 -0.010378667965007082 1106 32-33 -0.25606146345086955 1106 34-35 -0.08765781484898127 1106 36-37 -0.08810311065983711 1106 38-39 0.23976619616116324 1106 40-41 0.30171378465009724 1106 42-43 0.3359397454856179 1106 44-45 0.5440257099892989 1106 46-47 -0.0180377077344005 1106 48-49 0.18851074949428437 1106 50-51 0.13937703763554055 1106 52-53 0.13250661862740287 1106 54-55 0.28088392437140897 1106 56-57 -0.3064895257986038 1106 58-59 -0.0778441706318489 1106 60-61 -0.3801605652349167 1106 62-63 -0.2066726437015518 1106 64-65 -0.2755191360060536 1106 66-67 -0.1661149197728804 1106 68-69 -0.56274517846024 1106 70-71 -0.08964586198021607 1106 72-73 -0.3777456174941811 1106 74-75 -0.7132987826246051 1106 76-77 -0.34756262140445315 1106 78-79 6.431660048988874E-4 1106 80-81 -0.13139400415300173 1106 82-83 -0.23227694303193047 1106 84-85 -0.1016436443420119 1106 86-87 -0.2235156597916763 1106 88-89 -0.4470136073278077 1106 90-91 -0.3612357652660769 1106 92-93 -0.14209073676109085 1106 94-95 -0.1026806363913586 1106 96-97 0.10119796656494984 1106 98-99 -0.1964131571785046 1106 100-101 0.03799895962892563 1107 1 -0.18554188897186208 1107 2 -0.0644037475480701 1107 3 -0.3181683908834856 1107 4 -0.19844351651895664 1107 5 -0.16654156567894773 1107 6 0.02823829301977554 1107 7 -0.02525641950823143 1107 8 0.04018481122548678 1107 9 -0.0923651143254034 1107 10-11 -0.013613343336153605 1107 12-13 0.10976739637050059 1107 14-15 -0.019854870550489068 1107 16-17 -0.13782067771246176 1107 18-19 0.12028203241627011 1107 20-21 0.26625462384948406 1107 22-23 0.03859941510388154 1107 24-25 0.1210091310272432 1107 26-27 -0.14675227082295095 1107 28-29 0.003516489372508147 1107 30-31 0.118980575732472 1107 32-33 0.1430675631597751 1107 34-35 0.12970913192972944 1107 36-37 0.10599265264549018 1107 38-39 -0.08038436406292249 1107 40-41 0.23154571742319519 1107 42-43 -0.12114621389813607 1107 44-45 0.16822914136184863 1107 46-47 0.023130009352435366 1107 48-49 0.06658322848587517 1107 50-51 -0.1514886836529854 1107 52-53 0.048669958963543536 1107 54-55 0.10740265686440864 1107 56-57 -0.22203136753530117 1107 58-59 -0.055789723853138184 1107 60-61 -0.3718797529099902 1107 62-63 -0.02252961148867172 1107 64-65 0.159376697327275 1107 66-67 0.010023035409190584 1107 68-69 0.12152915627365246 1107 70-71 0.023064222053395866 1107 72-73 -0.5651318219759744 1107 74-75 -0.10131086245653975 1107 76-77 -0.2737530975949305 1107 78-79 -0.08528120374299775 1107 80-81 -0.08993304476924635 1107 82-83 -0.09430425395630593 1107 84-85 -0.2073421737537764 1107 86-87 0.07503563572653249 1107 88-89 -0.03829511993285095 1107 90-91 -0.6252151070027736 1107 92-93 -0.6755159818591281 1107 94-95 -0.4706997190244131 1107 96-97 -0.7142300796535359 1107 98-99 -0.45920639947542696 1107 100-101 -0.02178745493690215 1108 1 -0.1000574215162402 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8.0 6 13.0 7 61.0 8 130.0 9 287.0 10 488.0 11 904.0 12 1598.0 13 2358.0 14 2642.0 15 3335.0 16 4182.0 17 5773.0 18 5352.0 19 4013.0 20 3771.0 21 3818.0 22 4101.0 23 4243.0 24 4775.0 25 5352.0 26 6389.0 27 7842.0 28 9698.0 29 11969.0 30 15154.0 31 18804.0 32 23828.0 33 30785.0 34 42249.0 35 61209.0 36 95735.0 37 118520.0 38 76528.0 39 12264.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.1880169155346 16.87062609932263 11.447924853111784 15.493432132030987 2 14.29479812027774 32.37352339111688 31.96428935753487 21.367389131070503 3 38.970440651673236 22.914533374610055 23.810771182952262 14.304254790764448 4 12.934535902819691 15.418108248255225 45.932147198585476 25.715208650339605 5 11.071346600134312 44.932631740013775 32.15484923960999 11.841172420241932 6 25.741050859848517 44.504535137754274 13.617654309443457 16.13675969295375 7 31.98194445625101 33.98726591123541 18.8600525344917 15.170737098021881 8 22.338719960556627 24.865135969125358 17.872438093456992 34.92370597686102 9 19.55116162431888 33.21829016380901 21.313532307012252 25.917015904859863 10-11 27.945629351309538 22.931900677507926 27.12938956280762 21.993080408374915 12-13 39.442522335659696 21.344219930804083 20.52228465533803 18.690973078198187 14-15 23.410924015424058 28.37681834565399 32.26131131709772 15.950946321824228 16-17 18.173703851679317 27.16942798609281 31.380688048828176 23.276180113399697 18-19 17.337317340632623 40.542516385150925 25.205165041611053 16.915001232605388 20-21 25.076111232496118 24.776597273173223 23.17581715960787 26.97147433472279 22-23 19.077161097273816 30.130061120225783 23.373428428130605 27.4193493543698 24-25 29.650535120752824 28.688762889227025 23.765906985047224 17.894795004972924 26-27 28.846541307169872 21.375383937900036 33.615830015718814 16.16224473921127 28-29 18.84096906711647 38.68710381696003 26.74524580352042 15.726681312403079 30-31 24.234535773523756 26.38425432753495 24.124779914422735 25.256429984518558 32-33 24.461193705028634 31.08864715243678 24.751594825827336 19.698564316707245 34-35 29.1885963980124 23.611764055785326 28.97512953808356 18.22451000811871 36-37 27.050211072708493 22.357212394835628 32.41337091405324 18.179205618402648 38-39 20.539867971417053 29.771833108523005 33.1573405816946 16.53095833836534 40-41 20.172401334665167 31.675004781841753 25.986100779971522 22.16649310352156 42-43 17.47643306036945 34.58703444241913 21.384022091030282 26.552510406181135 44-45 25.909511356564497 22.397546070686495 23.233432746489648 28.459509826259357 46-47 30.032278604224526 31.262385012254306 22.680968885193753 16.024367498327415 48-49 29.035274515554537 29.180980069622432 24.62024321096277 17.16350220386026 50-51 17.53854131688677 30.401033719136127 35.131658923690956 16.928766040286142 52-53 25.12447347951936 36.11503330202193 21.408673402161764 17.351819816296953 54-55 18.42187363189916 30.741518093571933 23.885181114236577 26.951427160292322 56-57 26.16580971618616 24.038821217005708 27.428451436072177 22.36691763073596 58-59 36.11610091754818 23.6106494418454 21.013579169029327 19.25967047157709 60-61 22.844553863731615 29.40344857779958 32.89121669354448 14.860780864924328 62-63 19.117749572816688 26.81478521818228 31.393595225748317 22.673869983252715 64-65 17.961759706817613 40.438140251838774 24.096437813586228 17.50366222775739 66-67 24.63861778904412 25.444164864609757 22.15624364886549 27.760973697480633 68-69 19.61500067173786 31.10588459373464 22.30943477085236 26.969679963675137 70-71 29.47344436405312 29.17145259570937 22.34441515026046 19.010687889977053 72-73 28.596809334486036 22.45621909451881 31.264849719773302 17.68212185122185 74-75 19.439665138800102 38.87916026277492 25.23717068128341 16.444003917141575 76-77 24.391579144228196 26.879922473041955 22.747021545183678 25.981476837546168 78-79 24.305551423240235 31.561802671789792 22.93871374955902 21.19393215541095 80-81 28.674482281404636 24.884410679356627 26.575640342309303 19.865466696929435 82-83 26.579103066737847 23.558779425415942 30.42808178420965 19.434035723636562 84-85 20.239993743433153 30.018514677429774 31.077880655987432 18.663610923149637 86-87 20.188946469984153 31.447864172090394 24.203867681363448 24.159321676562 88-89 18.084376585918193 34.2299907597021 20.717478954307705 26.968153700072 90-91 25.554888716228508 21.865038143020602 23.02760544798285 29.55246769276804 92-93 28.266372880549078 30.26141523981482 22.611670177478775 18.86054170215732 94-95 27.361615852492253 28.52768533040902 24.74240125133358 19.368297565765143 96-97 16.91838559837866 29.86877631104673 35.116533708743944 18.09630438183067 98-99 23.847978372496346 35.700513483184274 21.661424150712417 18.790083993606967 100-101 16.893628959583765 32.132011630081784 22.758063693401116 28.21629571693333 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 98.0 1 80.0 2 138.5 3 214.0 4 169.0 5 148.0 6 182.0 7 289.0 8 413.5 9 487.0 10 517.0 11 496.5 12 528.0 13 605.5 14 676.0 15 720.5 16 798.5 17 887.5 18 905.5 19 964.0 20 1045.5 21 1070.5 22 1170.0 23 1344.0 24 1520.0 25 1722.0 26 2050.0 27 2407.5 28 2995.0 29 3772.0 30 4422.0 31 5045.0 32 5560.5 33 6131.0 34 6847.0 35 7580.5 36 8316.5 37 8946.5 38 9677.5 39 10238.0 40 10461.5 41 10938.5 42 11602.5 43 12388.0 44 12825.0 45 13544.5 46 17512.5 47 45375.5 48 111535.0 49 110605.5 50 47453.0 51 20791.5 52 12677.5 53 9648.0 54 8075.5 55 6845.5 56 5749.0 57 4526.0 58 3549.5 59 2867.0 60 2252.0 61 1698.5 62 1264.5 63 966.5 64 664.0 65 447.0 66 304.5 67 191.0 68 127.5 69 79.0 70 40.5 71 21.0 72 13.5 73 7.0 74 1.5 75 2.0 76 1.5 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05491469520644015 2 0.0022101889711570338 3 0.048114113756726196 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0022101889711570338 16-17 0.0 18-19 0.0 20-21 0.011135952123906594 22-23 0.0 24-25 0.0 26-27 0.006205530572863979 28-29 0.012411061145727958 30-31 0.0104558939789352 32-33 0.005610479696014009 34-35 0.006375545109106828 36-37 0.019976708008534728 38-39 0.04794409922048335 40-41 0.003825327065464097 42-43 0.012411061145727958 44-45 0.011390973928270867 46-47 0.004165356137949794 48-49 0.002125181703035609 50-51 0.0029752543842498534 52-53 0.0012751090218213659 54-55 0.0011050944855785169 56-57 0.009010770420870983 58-59 0.0070556032540782234 60-61 0.005015428819164038 62-63 0.0051004360872854634 64-65 0.07276622151193927 66-67 0.03391789998044833 68-69 0.026862296726370104 70-71 0.015216300993734964 72-73 8.500726812142438E-5 74-75 1.7001453624284875E-4 76-77 4.250363406071219E-4 78-79 4.250363406071219E-4 80-81 5.950508768499706E-4 82-83 5.100436087285464E-4 84-85 5.100436087285464E-4 86-87 0.004930421551042614 88-89 8.500726812142438E-5 90-91 6.80058144971395E-4 92-93 3.400290724856975E-4 94-95 0.002125181703035609 96-97 0.004930421551042614 98-99 0.007650654130928194 100-101 0.009350799493356682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 588185.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.6147575709641 #Duplication Level Percentage of deduplicated Percentage of total 1 87.08974887749157 35.37129037575464 2 7.749875581214222 6.295186358723003 3 1.999129978140549 2.435825382450755 4 0.9138014042721601 1.4845529001008135 5 0.5135294804799184 1.0428437677617515 6 0.33023521773712866 0.8047453985872816 7 0.22283787114800538 0.6335354280014183 8 0.16979828380655942 0.5517052906215337 9 0.1312755269910364 0.47985513333673485 >10 0.7642617525027461 5.710919607382058 >50 0.05242524437119618 1.4840619868585516 >100 0.0421959283962529 3.4635614409222013 >500 0.005540879486376643 1.6975051724151893 >1k 0.010655537473801236 9.453126907530967 >5k 0.0025573289937122967 8.235306298930023 >10k+ 0.0021311074947602472 20.855978550623046 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 69139 11.754635021294321 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 17801 3.026428759658951 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 13153 2.23620119520219 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10229 1.7390786912281 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10157 1.7268376446186149 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 9170 1.5590332973469232 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9011 1.5320009860843102 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8476 1.441043209194386 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8080 1.373717452842218 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6489 1.1032243256798457 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6347 1.079082261533361 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4977 0.8461623468806584 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4443 0.755374584526977 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4153 0.706070369016551 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3772 0.6412948307080255 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3574 0.6076319525319415 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3113 0.5292552513239882 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2518 0.4280966022594932 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2321 0.394603738619652 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1949 0.3313583311373122 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1861 0.31639705194794154 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1795 0.3051760925559135 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1763 0.29973562739614235 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1720 0.2924250023376999 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1707 0.2902148133665428 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1644 0.27950389758324334 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1639 0.2786538249020291 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1542 0.2621624148864728 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1503 0.2555318479730017 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1469 0.2497513537407448 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1397 0.23751030713125973 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 1269 0.21574844649217506 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 1167 0.1984069637954045 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1158 0.19687683296921885 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1108 0.18837610615707645 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1046 0.1778352049100198 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 981 0.16678426005423463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 957 0.16270391118440627 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 942 0.16015369314076355 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 856 0.14553244302387855 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 803 0.13652167260300754 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 755 0.1283609748633508 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 743 0.12632080042843663 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 709 0.12054030619617978 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 675 0.11475981196392292 No Hit AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 611 0.1038788816443806 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 600 0.10200872174570925 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 595 0.10115864906449501 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.400290724856975E-4 0.0 10-11 0.0 0.0 0.0 4.250363406071219E-4 0.0 12-13 0.0 0.0 0.0 9.350799493356681E-4 0.0 14-15 0.0 0.0 0.0 0.0014451235580642144 0.0 16-17 0.0 0.0 0.0 0.00272023257988558 0.0 18-19 0.0 0.0 0.0 0.00408034886982837 0.0 20-21 0.0 0.0 0.0 0.005355457891649736 0.0 22-23 0.0 0.0 0.0 0.007905675935292468 0.0 24-25 0.0 0.0 0.0 0.011135952123906594 0.0 26-27 0.0 0.0 0.0 0.014196213776277871 0.0 28-29 0.0 0.0 0.0 0.026607274922005833 0.0 30-31 0.0 0.0 0.0 0.050239295459761815 0.0 32-33 0.0 0.0 0.0 0.10931934680415176 0.0 34-35 0.0 0.0 0.0 0.15998367860452067 0.0 36-37 0.0 0.0 0.0 0.18506082270034085 0.0 38-39 0.0 0.0 0.0 0.20937290138306824 0.0 40-41 0.0 0.0 0.0 0.24320579409539517 0.0 42-43 0.0 0.0 0.0 0.2977804602293496 0.0 44-45 0.0 0.0 0.0 0.33892397800011903 0.0 46-47 0.0 0.0 0.0 0.3890782661917594 0.0 48-49 0.0 0.0 0.0 0.41415541028757963 0.0 50-51 0.0 0.0 0.0 0.4520686518697349 0.0 52-53 0.0 0.0 0.0 0.6160476720759624 0.0 54-55 0.0 0.0 0.0 0.7928627897685252 0.0 56-57 0.0 0.0 0.0 0.9207987282912689 0.0 58-59 0.0 0.0 0.0 1.1060295655278525 0.0 60-61 0.0 0.0 0.0 1.3343590877019986 0.0 62-63 0.0 0.0 0.0 1.4101855708663091 0.0 64-65 0.0 0.0 0.0 1.4831218069144911 0.0 66-67 0.0 0.0 0.0 2.0708620587060196 0.0 68-69 0.0 0.0 0.0 2.493603203073863 0.0 70-71 0.0 0.0 0.0 2.575550209542916 0.0 72-73 0.0 0.0 0.0 2.7765923986500844 0.0 74-75 0.0 0.0 0.0 3.0879740217788623 0.0 76-77 0.0 0.0 0.0 3.3204689000909577 0.0 78-79 0.0 0.0 0.0 3.568095072128667 0.0 80-81 0.0 0.0 0.0 4.056206805681885 0.0 82-83 0.0 0.0 0.0 4.437634417742717 0.0 84-85 0.0 0.0 0.0 4.771287945119308 0.0 86-87 0.0 0.0 0.0 6.079804823312394 0.0 88-89 0.0 0.0 0.0 7.865467497471034 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 115 0.0 66.08914 3 TATAACG 125 0.0 57.006737 2 TACCTGG 650 0.0 50.42903 2 ACCTGGG 710 0.0 45.49446 3 TAACGCA 165 0.0 43.168564 4 TACGCAG 1000 0.0 42.736877 4 AGTACGC 990 0.0 41.747356 2 GAGTACG 1005 0.0 41.625275 1 TATCACG 80 2.2579843E-7 41.567413 2 GTATAAC 185 0.0 41.113907 1 ACGCAGT 1085 0.0 38.951176 5 GTACGCA 1065 0.0 38.804104 3 CGCAGTG 1090 0.0 38.7725 6 GTACCTG 875 0.0 38.030365 1 GTACACG 50 0.0016088472 38.030365 1 GTAGGCA 625 0.0 37.241234 3 TAGGCAG 610 0.0 36.587128 4 TCACGCA 95 8.711213E-7 34.989258 4 GAGTAGG 695 0.0 34.19997 1 GTACCCG 70 2.0425089E-4 33.955685 1 >>END_MODULE