##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003791_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 132640 Sequences flagged as poor quality 0 Sequence length 121 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.697233112183355 31.0 31.0 34.0 27.0 34.0 2 30.475821773220748 31.0 31.0 34.0 26.0 34.0 3 30.649796441495777 33.0 31.0 34.0 26.0 34.0 4 33.98853287092883 37.0 35.0 37.0 30.0 37.0 5 33.86865199034982 37.0 35.0 37.0 28.0 37.0 6 33.961044933655 37.0 35.0 37.0 29.0 37.0 7 33.90471954161641 37.0 35.0 37.0 29.0 37.0 8 34.00648371531966 37.0 35.0 37.0 30.0 37.0 9 35.46157267792521 39.0 35.0 39.0 30.0 39.0 10-11 35.35048250904704 39.0 35.0 39.0 29.0 39.0 12-13 35.32990425211098 39.0 35.0 39.0 29.5 39.0 14-15 36.3023974668275 40.0 36.0 41.0 28.0 41.0 16-17 36.283760554885404 40.0 36.0 41.0 27.5 41.0 18-19 36.22807976477684 40.0 36.0 41.0 27.0 41.0 20-21 36.19924231001206 40.0 36.0 41.0 27.0 41.0 22-23 36.01879523522316 39.5 36.0 41.0 27.0 41.0 24-25 35.88141586248492 39.0 36.0 41.0 27.0 41.0 26-27 35.71340847406514 39.0 35.5 41.0 26.5 41.0 28-29 35.575448582629676 39.0 35.0 41.0 27.0 41.0 30-31 35.23980699638118 39.0 35.0 40.0 25.0 41.0 32-33 34.968410735826296 38.0 35.0 40.0 25.0 41.0 34-35 34.68099743667069 38.0 34.0 40.0 23.5 41.0 36-37 34.68222255729795 38.0 34.0 40.0 24.0 41.0 38-39 34.54448507237636 38.0 34.0 40.0 24.0 41.0 40-41 34.73093335343788 39.0 35.0 40.5 24.0 41.0 42-43 34.59075693606755 39.0 35.0 40.5 23.0 41.0 44-45 34.329244571773216 38.0 34.0 40.0 19.5 41.0 46-47 33.93547195416164 38.0 33.5 40.0 15.0 41.0 48-49 33.55055036188179 38.0 33.0 40.0 11.0 41.0 50-51 32.77043501206272 37.5 32.0 39.5 9.0 40.5 52-53 32.857003920386006 38.0 33.0 39.5 8.0 40.5 54-55 33.15828558504222 38.0 33.0 40.0 7.0 41.0 56-57 32.7020205066345 38.0 32.5 40.0 2.0 41.0 58-59 32.356920989143546 38.0 31.5 40.0 2.0 41.0 60-61 31.778068455971052 37.0 31.0 40.0 2.0 41.0 62-63 31.62516209288299 37.0 31.0 40.0 2.0 41.0 64-65 31.27516963208685 36.0 30.5 39.5 2.0 41.0 66-67 30.768749999999997 36.0 29.5 39.0 2.0 41.0 68-69 30.377288148371534 35.5 29.0 39.0 2.0 40.0 70-71 29.723137816646563 35.0 28.0 38.0 2.0 40.0 72-73 29.28623718335344 35.0 28.0 37.0 2.0 39.5 74-75 28.85524728588661 34.0 27.0 37.0 2.0 39.0 76-77 28.37739369722557 34.0 26.0 36.5 2.0 39.0 78-79 27.65305714716526 34.0 26.0 36.0 2.0 38.0 80-81 27.154576296743066 34.0 25.0 35.5 2.0 37.0 82-83 26.658892490952958 33.0 23.5 35.0 2.0 37.0 84-85 26.352714113389624 33.0 23.0 35.0 2.0 36.5 86-87 26.291887816646565 33.0 23.0 35.0 2.0 36.0 88-89 25.909721803377565 33.0 20.5 35.0 2.0 36.0 90-91 25.617110223160434 33.0 20.0 35.0 2.0 35.0 92-93 25.302461550060315 32.5 19.0 35.0 2.0 35.0 94-95 25.010524728588663 32.0 18.0 35.0 2.0 35.0 96-97 24.72070265379976 32.0 14.5 35.0 2.0 35.0 98-99 24.36015907720145 32.0 8.5 35.0 2.0 35.0 100-101 24.000938630880576 32.0 7.0 35.0 2.0 35.0 102-103 23.543482358262967 31.0 2.0 34.0 2.0 35.0 104-105 22.991782267792523 31.0 2.0 34.0 2.0 35.0 106-107 22.39168425814234 30.0 2.0 34.0 2.0 35.0 108-109 22.13119722557298 30.0 2.0 34.0 2.0 35.0 110-111 21.75631031363088 29.5 2.0 34.0 2.0 35.0 112-113 21.06359318455971 29.0 2.0 34.0 2.0 35.0 114-115 20.375776537997588 29.0 2.0 34.0 2.0 35.0 116-117 19.513321773220746 27.0 2.0 34.0 2.0 35.0 118-119 18.720378468033775 25.0 2.0 34.0 2.0 35.0 120-121 17.22723537394451 21.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3465260361782398 1101 2 1.5071231423020315 1101 3 1.515958833533933 1101 4 1.712682175788757 1101 5 1.8227493421298036 1101 6 1.7388544770014178 1101 7 1.3885540344777922 1101 8 1.5706917715182485 1101 9 1.658331364421521 1101 10-11 1.7264417239441414 1101 12-13 1.5935728215130922 1101 14-15 2.2997672242136744 1101 16-17 2.1139891890880946 1101 18-19 1.7988097036669544 1101 20-21 1.8560350495767395 1101 22-23 1.7627792892933414 1101 24-25 1.8500928313941003 1101 26-27 1.9681360372650971 1101 28-29 2.1636678550788986 1101 30-31 1.718930956106341 1101 32-33 2.2491300298460217 1101 34-35 2.4933867344233107 1101 36-37 2.017687636345478 1101 38-39 2.0578341784679495 1101 40-41 2.255223673194571 1101 42-43 2.7303995763772306 1101 44-45 2.5890093157288376 1101 46-47 2.867718447059744 1101 48-49 3.0610530404239213 1101 50-51 2.4604279369875357 1101 52-53 2.347883825729305 1101 54-55 2.57413735556748 1101 56-57 2.9729897117516053 1101 58-59 2.5447501461915003 1101 60-61 2.1678070528497067 1101 62-63 2.3557344992949147 1101 64-65 2.791144826510603 1101 66-67 2.466548985049716 1101 68-69 2.472234891592109 1101 70-71 2.3627075187654683 1101 72-73 2.2329456870757234 1101 74-75 2.590954932288838 1101 76-77 2.5565213364350576 1101 78-79 2.7087656685527435 1101 80-81 2.189660533962506 1101 82-83 1.9559962423647157 1101 84-85 2.5762025731513987 1101 86-87 2.351066517517129 1101 88-89 2.415788956029356 1101 90-91 1.9270307444184027 1101 92-93 2.220223411374551 1101 94-95 2.0058869195492797 1101 96-97 2.2206650343031704 1101 98-99 2.469696017104706 1101 100-101 2.6776642589819843 1101 102-103 1.8960743225065002 1101 104-105 1.8339074418623973 1101 106-107 1.1924475813738482 1101 108-109 1.1762805733117325 1101 110-111 1.1915886141362577 1101 112-113 2.210126446434977 1101 114-115 1.9649910796389776 1101 116-117 1.1575046129823754 1101 118-119 1.4429959447804812 1101 120-121 1.8914264992137326 1102 1 0.5029546076068101 1102 2 0.4389088565877479 1102 3 0.27988740496250486 1102 4 0.7260750329316181 1102 5 0.8677493421298053 1102 6 1.0243901912871323 1102 7 1.1685540344777934 1102 8 1.1040846286611057 1102 9 1.1885099358500923 1102 10-11 1.216531009658432 1102 12-13 1.216697821513094 1102 14-15 1.0658386527851036 1102 16-17 1.3246141890880878 1102 18-19 1.4849704179526668 1102 20-21 1.7980886210053129 1102 22-23 1.6673328607219204 1102 24-25 1.6910749742512436 1102 26-27 1.524564608693673 1102 28-29 1.517060712221756 1102 30-31 1.7808059561063416 1102 32-33 1.4153800298460197 1102 34-35 1.8813331629947427 1102 36-37 1.549919779202618 1102 38-39 1.7980127498965217 1102 40-41 2.279777244623155 1102 42-43 1.8810245763772286 1102 44-45 1.602580744300269 1102 46-47 2.2320934470597464 1102 48-49 2.3628387547096423 1102 50-51 1.9651600798446793 1102 52-53 2.000919540015012 1102 54-55 2.101726641281765 1102 56-57 2.3204004260373203 1102 58-59 2.283589431905785 1102 60-61 2.3478070528497135 1102 62-63 2.407163070723488 1102 64-65 2.5036448265106017 1102 66-67 2.837173985049713 1102 68-69 2.2660741773063933 1102 70-71 2.808421804479753 1102 72-73 2.7643742585042936 1102 74-75 2.7046156465745526 1102 76-77 2.659199907863627 1102 78-79 2.7966228114098897 1102 80-81 3.038499819676794 1102 82-83 2.6143890995075765 1102 84-85 2.9770954302942556 1102 86-87 2.4683879460885585 1102 88-89 2.879896098886501 1102 90-91 2.7683700301326866 1102 92-93 2.28299126851741 1102 94-95 2.574369062406422 1102 96-97 2.6888793200174526 1102 98-99 2.65398173139042 1102 100-101 2.6048071161248387 1102 102-103 2.9192886082207856 1102 104-105 2.617211013290966 1102 106-107 3.357269009945277 1102 108-109 2.822441287597446 1102 110-111 2.6923921855648274 1102 112-113 3.169322875006408 1102 114-115 2.1406160796389777 1102 116-117 1.7500046129823765 1102 118-119 2.4475495162090546 1102 120-121 2.996783642070877 1103 1 0.9856469152991174 1103 2 1.2014088565877472 1103 3 0.9904643280394296 1103 4 1.1775173406239219 1103 5 1.088903188283652 1103 6 1.1008324989794431 1103 7 1.3608617267854797 1103 8 1.1478346286611014 1103 9 1.3312983973885508 1103 10-11 1.270617548119965 1103 12-13 1.513572821513094 1103 14-15 1.9591078835543385 1103 16-17 1.7416814967803944 1103 18-19 1.520787725644972 1103 20-21 1.680540544082234 1103 22-23 1.6834386299526898 1103 24-25 1.1937192050204786 1103 26-27 1.8957184548475183 1103 28-29 1.560810712221759 1103 30-31 1.7320078791832643 1103 32-33 1.6937454144614108 1103 34-35 1.833496624533204 1103 36-37 1.6713140099718515 1103 38-39 1.60233967297345 1103 40-41 1.7381907061616104 1103 42-43 2.1750149609926126 1103 44-45 2.058349975069497 1103 46-47 2.1400261393674356 1103 48-49 2.3945695239404046 1103 50-51 2.8279004644600576 1103 52-53 1.8667849246304016 1103 54-55 2.6952362566663837 1103 56-57 2.767275426037312 1103 58-59 2.3535413549827133 1103 60-61 2.1093455143881776 1103 62-63 2.1494707630311787 1103 64-65 2.1257602111259857 1103 66-67 2.192702831203558 1103 68-69 2.2802568696140852 1103 70-71 1.2391910352489859 1103 72-73 1.9989896431196819 1103 74-75 2.6183175696514738 1103 76-77 2.985642215555938 1103 78-79 2.863930503717576 1103 80-81 2.877682511984485 1103 82-83 3.4216006379691137 1103 84-85 2.794883891832715 1103 86-87 2.4727148691654826 1103 88-89 2.0294153296557305 1103 90-91 2.221975799363456 1103 92-93 2.3250585762097167 1103 94-95 2.9510517547141184 1103 96-97 3.684071627709759 1103 98-99 3.6078278852365777 1103 100-101 3.3317301930479175 1103 102-103 3.462557838990019 1103 104-105 3.0645667825217373 1103 106-107 3.347172856099121 1103 108-109 3.4044124414436006 1103 110-111 2.609940262487907 1103 112-113 1.724851721160249 1103 114-115 2.4442218488697485 1103 116-117 3.3528892283669904 1103 118-119 3.859809131593668 1103 120-121 3.1765913343785677 1104 1 1.5163384459906482 1104 2 1.2852472404261306 1104 3 1.989609627184727 1104 4 2.026390689497269 1104 5 1.7649715643520238 1104 6 1.8062715044184472 1104 7 1.8826954486192022 1104 8 1.9942992751257478 1104 9 2.637436703526859 1104 10-11 2.3806408581432805 1104 12-13 1.8368051447454121 1104 14-15 1.7899548144012627 1104 16-17 2.0733831284820283 1104 18-19 2.2888529937102433 1104 20-21 1.9640292775709725 1104 22-23 1.8121876587017098 1104 24-25 2.0018388631401294 1104 26-27 2.496534305663374 1104 28-29 2.0163662677773146 1104 30-31 1.394284491459878 1104 32-33 2.210140130856125 1104 34-35 2.0694329104694873 1104 36-37 2.1789286175864575 1104 38-39 1.8979496185833895 1104 40-41 1.896349214320118 1104 42-43 2.661106647084303 1104 44-45 2.387681754401278 1104 46-47 2.187112386453684 1104 48-49 2.684429663800543 1104 50-51 1.851365887925482 1104 52-53 1.9417654996109732 1104 54-55 1.9172253786555018 1104 56-57 1.3995986583605493 1104 58-59 1.5254139268552827 1104 60-61 1.7235646286072885 1104 62-63 2.1920115555719697 1104 64-65 2.030538765904538 1104 66-67 2.1617005002012313 1104 68-69 2.488454227811445 1104 70-71 2.154886450944396 1104 72-73 2.036091430221468 1104 74-75 2.2492810506149574 1104 76-77 2.454212534126256 1104 78-79 1.4211177609048349 1104 80-81 1.0656778499798243 1104 82-83 1.1493007156691917 1104 84-85 1.0505171474659711 1104 86-87 0.7269106733612851 1104 88-89 0.4744036746440763 1104 90-91 0.020421797809454745 1104 92-93 0.436558187709327 1104 94-95 1.4996531533155135 1104 96-97 0.8879954816336166 1104 98-99 1.0191332465419372 1104 100-101 -0.0906474293297066 1104 102-103 0.6888593152914915 1104 104-105 0.23725520521015753 1104 106-107 0.25758466651093315 1104 108-109 -0.523171086139925 1104 110-111 -0.07657877403113034 1104 112-113 -1.4465546502461208 1104 114-115 -1.4760190213711262 1104 116-117 -1.552394376916613 1104 118-119 -0.47931285752832054 1104 120-121 -0.35864565085841704 1105 1 0.7537121833643852 1105 2 1.204439159618051 1105 3 1.2825389201140212 1105 4 1.5061886692952484 1105 5 1.3760826754631381 1105 6 1.8416250397719836 1105 7 1.7564328223565795 1105 8 1.4084406892671666 1105 9 1.3849114510016065 1105 10-11 1.4412469187493357 1105 12-13 1.7787243366646095 1105 14-15 1.295005319451775 1105 16-17 1.770352825451731 1105 18-19 1.2585499634072121 1105 20-21 0.9135242270659205 1105 22-23 1.8020866486006994 1105 24-25 1.847798459099728 1105 26-27 1.0268373359663983 1105 28-29 0.9911137425247887 1105 30-31 1.775597622773013 1105 32-33 1.404584575300568 1105 34-35 1.2386248296614042 1105 36-37 1.1814538701117172 1105 38-39 1.301990022623798 1105 40-41 1.858470426441336 1105 42-43 2.02221775819541 1105 44-45 2.7487928655123923 1105 46-47 2.7174154167567153 1105 48-49 2.305641785012668 1105 50-51 2.2554062919658904 1105 52-53 2.5958059036513816 1105 54-55 2.9753061867363115 1105 56-57 1.7960633048251928 1105 58-59 2.068343219784577 1105 60-61 1.526594931637586 1105 62-63 1.6617085252689385 1105 64-65 2.1972054325712094 1105 66-67 2.6844277729285047 1105 68-69 2.263706753063971 1105 70-71 2.114482410540358 1105 72-73 1.6825560766861152 1105 74-75 1.6103921617260681 1105 76-77 2.532495362409083 1105 78-79 2.9791985689856446 1105 80-81 3.4823445166464886 1105 82-83 3.1088966752651537 1105 84-85 3.2272848242336494 1105 86-87 2.5678197642703786 1105 88-89 3.163797614038014 1105 90-91 2.2148662422538976 1105 92-93 2.1006996018507422 1105 94-95 1.1208652745276346 1105 96-97 1.688500532138665 1105 98-99 1.6327696101783005 1105 100-101 2.250261661579387 1105 102-103 2.436334062766239 1105 104-105 3.1059420738970296 1105 106-107 2.6010190099452757 1105 108-109 2.214202651233812 1105 110-111 1.4815020340496794 1105 112-113 0.9423342386427684 1105 114-115 1.2159001705480712 1105 116-117 1.3390197644975288 1105 118-119 1.6292730010575376 1105 120-121 1.5024654602526972 1106 1 -0.6994038829592277 1106 2 0.24103149809717905 1106 3 -0.572235236546927 1106 4 -0.4792315708419679 1106 5 0.12765500250716144 1106 6 -0.3370484879581497 1106 7 0.6949691288174122 1106 8 -0.06725971096153671 1106 9 -0.33778723396122956 1106 10-11 -0.49614587713401903 1106 12-13 -0.24114415961898317 1106 14-15 0.41135752070962894 1106 16-17 0.18115900040884725 1106 18-19 0.7508312670092678 1106 20-21 0.6093504134581451 1106 22-23 0.16515125694832733 1106 24-25 0.17898771010030146 1106 26-27 -0.31364293847613567 1106 28-29 0.49854656127835995 1106 30-31 1.0853460504459633 1106 32-33 1.3072432373931875 1106 34-35 0.5672411818626628 1106 36-37 -0.7349269189105812 1106 38-39 0.2934136932927487 1106 40-41 0.38891639556654667 1106 42-43 0.7979467461885505 1106 44-45 0.08512791411158815 1106 46-47 -0.1296400435062921 1106 48-49 0.4923198867851113 1106 50-51 1.2036624383352432 1106 52-53 0.15198086076973283 1106 54-55 0.8024931507157262 1106 56-57 1.289916935471279 1106 58-59 1.1908417903963517 1106 60-61 1.7421466754912238 1106 62-63 1.6977291084593311 1106 64-65 1.638314637831357 1106 66-67 1.6465489850497157 1106 68-69 1.2209680452309222 1106 70-71 2.215968974291073 1106 72-73 1.5436195415231637 1106 74-75 1.5056180050651164 1106 76-77 0.16757254937306598 1106 78-79 -0.16988662255237585 1106 80-81 0.8844314234503763 1106 82-83 1.136912684413236 1106 84-85 -0.06288098480008486 1106 86-87 0.9902039838244043 1106 88-89 1.191098929075178 1106 90-91 0.2999148414534396 1106 92-93 0.14000777795137154 1106 94-95 -0.19502952249923666 1106 96-97 -0.4906018120580171 1106 98-99 0.02284965591872279 1106 100-101 0.030278814238048568 1106 102-103 0.1799018157679555 1106 104-105 0.2811850698947431 1106 106-107 -0.4461508013754809 1106 108-109 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1.4098744515946287 2207 4 1.6444364318953433 2207 5 1.910624989798194 2207 6 1.9698241291109682 2207 7 2.2353934127161352 2207 8 1.6690781519771676 2207 9 1.5381537182335094 2207 10-11 1.9259383930781198 2207 12-13 1.899583184207394 2207 14-15 1.8381832123705948 2207 16-17 1.5692223497098539 2207 18-19 2.0628363505951555 2207 20-21 1.8566864189327674 2207 22-23 1.7421871353333174 2207 24-25 2.05595515559839 2207 26-27 2.0275438833050714 2207 28-29 2.0100335101492135 2207 30-31 2.353749608956086 2207 32-33 2.6039486826957656 2207 34-35 2.4903519453781584 2207 36-37 2.3172287170264596 2207 38-39 2.1795218172540345 2207 40-41 2.7841651979910225 2207 42-43 2.8131975038383743 2207 44-45 2.745456391968659 2207 46-47 2.7114490170079293 2207 48-49 2.645805075953163 2207 50-51 2.3826633181866512 2207 52-53 2.790459695455432 2207 54-55 2.7938412785874576 2207 56-57 2.5273790529803186 2207 58-59 2.4475628775016425 2207 60-61 2.6866671564766555 2207 62-63 2.6942097028478393 2207 64-65 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1.552996187031681 2210 16-17 1.750427545252471 2210 18-19 1.3980269248019823 2210 20-21 1.3519841689505228 2210 22-23 1.188865394968495 2210 24-25 1.3492513441142577 2210 26-27 1.6043591292416153 2210 28-29 1.1902627670162786 2210 30-31 1.4582460245994895 2210 32-33 1.3899519476542395 2210 34-35 1.5274376150495357 2210 36-37 1.857668066873856 2210 38-39 2.1285778183896724 2210 40-41 2.412953614486163 2210 42-43 2.0424543708977794 2210 44-45 2.007717730601634 2210 46-47 2.422375981306324 2210 48-49 2.747941494435665 2210 50-51 2.454243983954271 2210 52-53 2.348607896179395 2210 54-55 2.059988627583138 2210 56-57 1.8659055630236132 2210 58-59 1.4516973086181189 2210 60-61 1.8258892446305346 2210 62-63 2.320176769353626 2210 64-65 2.5911448265106003 2210 66-67 2.913672272720955 2210 68-69 2.5338395882652947 2210 70-71 2.4159560510550975 2210 72-73 2.1623194639837457 2210 74-75 2.4651036602731793 2210 76-77 2.3450731955348623 2210 78-79 2.0640885648345417 2210 80-81 1.727155641594603 2210 82-83 2.1187555378637377 2210 84-85 2.597044060431241 2210 86-87 2.5852543844447204 2210 88-89 2.7548104824481427 2210 90-91 2.684251879447757 2210 92-93 3.342708734270836 2210 94-95 3.2888810487077933 2210 96-97 2.911310826866771 2210 98-99 2.1580913204315166 2210 100-101 2.800697527083745 2210 102-103 3.0297338137002363 2210 104-105 3.2679387530169954 2210 106-107 2.727731338712399 2210 108-109 3.175908753350871 2210 110-111 3.0268528020031873 2210 112-113 2.814999244869419 2210 114-115 2.9606075179951397 2210 116-117 2.889559407502926 2210 118-119 3.2129176668939827 2210 120-121 3.684283642070877 2211 1 0.6303355599877634 2211 2 1.0442659994448888 2211 3 1.3388159763910785 2211 4 1.3301424932490775 2211 5 0.5110033103837708 2211 6 1.1439338420807843 2211 7 1.0161730820968415 2211 8 0.9668822477087247 2211 9 1.2110297771199328 2211 10-11 1.32580680330922 2211 12-13 1.2913505992908654 2211 14-15 0.9854815099279577 2211 16-17 1.201449506548407 2211 18-19 0.8638890687463174 2211 20-21 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0.3140206530101253 2316 82-83 0.28313909950757576 2316 84-85 0.2239704302942549 2316 86-87 0.0907837794218942 2316 88-89 0.12781276555316623 2316 90-91 -0.40350496986731343 2316 92-93 0.3194496018507458 2316 94-95 0.18999406240642358 2316 96-97 0.14044182001745398 2316 98-99 0.27221089805708587 2316 100-101 0.5251196161248401 2316 102-103 0.9140802748874535 2316 104-105 0.9463776799576316 2316 106-107 0.7885190099452757 2316 108-109 0.4729621209307844 2316 110-111 0.776246352231496 2316 112-113 0.18286454167306943 2316 114-115 0.13019941297230986 2316 116-117 0.7161504463157122 2316 118-119 1.0715078495423889 2316 120-121 0.11657530873754496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3291.0 3 537.0 4 427.0 5 442.0 6 504.0 7 516.0 8 635.0 9 715.0 10 810.0 11 1031.0 12 1072.0 13 1266.0 14 1591.0 15 1652.0 16 1370.0 17 1334.0 18 1507.0 19 1522.0 20 1684.0 21 1778.0 22 1843.0 23 1958.0 24 2155.0 25 2377.0 26 2626.0 27 2980.0 28 3507.0 29 4044.0 30 4671.0 31 5377.0 32 6611.0 33 8086.0 34 10118.0 35 12910.0 36 16484.0 37 17190.0 38 5999.0 39 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.45095445095445 17.401625401625402 14.35796635796636 21.789453789453788 2 13.447369225595402 20.28187960500168 36.441040692164236 29.82971047723868 3 24.44693812119269 22.472862181102002 32.21194216705599 20.868257530649323 4 10.428436192628332 14.185268879592023 44.803340764039454 30.58295416374019 5 10.563520053741279 37.6211850562604 38.03951205361914 13.77578283637918 6 25.97407554429143 34.06668905920792 19.780756664325626 20.17847873217503 7 21.50593443498836 33.592336755333356 24.97194977674312 19.929779032935162 8 20.147180066566918 27.264954655103974 22.86100338941647 29.726861888912637 9 18.86187550093508 19.923667035609327 30.182054120071754 31.03240334338384 10-11 23.68321623076013 21.305544212105136 28.87554814308777 26.135691414046963 12-13 28.57011996137419 21.175491696596577 27.569360422288465 22.68502791974076 14-15 23.886732392108367 22.456574261733824 32.54058221596266 21.116111130195154 16-17 19.043856635749457 27.562884079545018 32.037100652696665 21.356158632008857 18-19 16.809815014162144 31.84306360367376 30.605498423459533 20.741622958704564 20-21 19.03378618244004 26.94573063430685 26.425825759339773 27.594657423913333 22-23 17.050455546522237 26.172073299668313 29.84469050699828 26.93278064681117 24-25 22.885389827273595 24.47103731272068 28.07710659414066 24.566466265865063 26-27 21.217593140492397 21.85742699747655 34.8602558571903 22.06472400484075 28-29 17.984224431514484 28.622806615659503 32.25172187352056 21.141247079305447 30-31 18.096507068709457 26.216264712956566 30.109304429826405 25.577923788507572 32-33 19.7190841793005 24.794887164078816 31.942625673369502 23.543402983251184 34-35 23.637370189370802 23.66905925473427 29.239462431276724 23.4541081246182 36-37 21.338225244245578 22.49464549553505 34.67504552760481 21.492083732614564 38-39 21.791817473771015 25.50472656190345 31.815717536384604 20.88773842794093 40-41 19.195629183068046 28.686349596631022 32.155361008556085 19.962660211744854 42-43 17.867354846591105 28.36680702584874 27.377675636674592 26.38816249088556 44-45 18.269487733551202 26.1279558972871 27.499293715210705 28.103262653950996 46-47 22.049087063099936 26.90853830822216 29.150598315400416 21.891776313277486 48-49 21.560048107210356 24.860737262088843 29.845178779374987 23.734035851325814 50-51 16.77032625088292 26.603287326040892 34.9223984880591 21.703987935017086 52-53 18.777490989064695 28.8033019732421 28.76206548964506 23.657141548048138 54-55 17.0395967772729 25.175455343846654 31.51895834128833 26.265989537592123 56-57 21.532515299971003 23.219327564366708 28.943271828212996 26.304885307449293 58-59 24.007569369994926 23.632916076899306 28.59726297074881 23.762251582356956 60-61 22.316782162151842 23.384685871370483 33.51736250310215 20.781169463375523 62-63 19.779099210577574 25.902374377952697 32.14060656057012 22.177919850899606 64-65 17.861862458273816 30.05507474429583 30.352600582065953 21.7304622153644 66-67 18.71555428244056 26.211782679270506 26.814857251981284 28.25780578630765 68-69 17.358231183411615 26.431053576201936 29.829686485660822 26.381028754725627 70-71 22.13303979574676 24.458790074760355 27.485132015471876 25.923038114021008 72-73 20.014984193968864 22.236238049914185 33.39602230810102 24.35275544801593 74-75 18.132370085813125 27.860787798864745 31.19737018137339 22.809471933948743 76-77 18.18862904890411 25.187872208673852 29.767520086845504 26.855978655576536 78-79 19.370239203773526 24.253919308797265 31.507884412828773 24.867957074600437 80-81 22.71506196959521 23.88462360339459 28.156862445814763 25.24345198119543 82-83 19.926300715990454 22.53021479713604 33.51369928400955 24.029785202863962 84-85 20.049407605105706 23.902360797699913 31.30433786554564 24.74389373164874 86-87 20.254827168837316 26.160830536496338 31.33373806332879 22.250604231337554 88-89 18.159670379605693 24.285065125994265 28.923884113534214 28.631380380865828 90-91 17.09774120252802 23.3162125522693 30.013556603593454 29.572489641609227 92-93 20.31016692189308 25.431625416731908 31.03082193088647 23.227385730488546 94-95 19.326851844780315 23.97297937175894 30.772432543895157 25.92773623956559 96-97 14.406443088344242 26.596833446624558 33.765876683138444 25.23084678189275 98-99 17.94749385111291 27.693670847400664 28.197421286606883 26.16141401487954 100-101 15.989718794517199 23.764403044642275 31.719352116011105 28.526526044829414 102-103 20.22819002088687 22.14886573216693 30.32376559355753 27.299178653388672 104-105 22.817105765559372 22.96869033982436 28.675830469644904 25.53837342497136 106-107 22.538583134149416 22.518346557109147 31.32431214729173 23.6187581614497 108-109 20.223453447834768 24.06286632886452 30.726376441927687 24.987303781373026 110-111 19.04019979607969 26.585633164927238 28.259383580214383 26.114783458778696 112-113 21.381140576970807 24.640586516982648 25.112549401454835 28.865723504591713 114-115 18.670026920213072 24.052160299368044 28.3571033087042 28.920709471714684 116-117 22.48689069917544 23.746071028922575 26.82394008486196 26.943098187040025 118-119 20.279001917040272 21.482689355462036 32.2837983365284 25.954510390969286 120-121 17.88297198017458 26.844886706429516 30.095155907532284 25.176985405863622 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 407.0 1 494.5 2 348.5 3 114.5 4 108.0 5 95.5 6 85.5 7 85.5 8 91.5 9 92.5 10 83.0 11 83.5 12 97.0 13 99.0 14 99.5 15 118.5 16 143.0 17 160.5 18 157.5 19 166.5 20 204.5 21 224.0 22 245.5 23 304.0 24 371.5 25 456.5 26 581.0 27 748.5 28 943.0 29 1188.5 30 1493.0 31 1729.0 32 1919.5 33 2189.0 34 2427.5 35 2546.5 36 2688.5 37 2949.5 38 3166.0 39 3231.0 40 3286.0 41 3445.5 42 3558.0 43 3676.5 44 3792.5 45 3980.0 46 5156.0 47 8927.0 48 16711.5 49 16753.5 50 8898.0 51 4814.0 52 3206.0 53 2563.0 54 2213.0 55 1899.5 56 1576.5 57 1303.0 58 1038.5 59 828.5 60 632.5 61 470.0 62 377.5 63 295.0 64 209.5 65 147.5 66 110.0 67 70.0 68 43.0 69 31.5 70 28.0 71 19.0 72 10.0 73 5.0 74 5.5 75 5.0 76 3.0 77 3.5 78 2.5 79 1.5 80 2.0 81 1.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2751809408926417 2 1.3600723763570566 3 1.237937273823884 4 1.2454764776839564 5 1.237937273823884 6 1.2394451145958987 7 1.2251206272617612 8 1.2394451145958987 9 1.2326598311218335 10-11 1.2285132689987937 12-13 1.2360524728588662 14-15 1.2368063932448734 16-17 1.2401990349819059 18-19 1.2492460796139926 20-21 1.2473612786489747 22-23 1.2390681544028952 24-25 1.2458534378769603 26-27 1.257916164053076 28-29 1.265078407720145 30-31 1.2631936067551266 32-33 1.2631936067551266 34-35 1.2665862484921593 36-37 1.2631936067551266 38-39 1.265078407720145 40-41 1.2669632086851628 42-43 1.2564083232810614 44-45 1.2613088057901087 46-47 1.2733715319662244 48-49 1.2684710494571771 50-51 1.2684710494571771 52-53 1.272617611580217 54-55 1.2778950542822678 56-57 1.2017490952955368 58-59 1.1953407720144753 60-61 1.2684710494571771 62-63 1.2978739445114595 64-65 1.3020205066344994 66-67 1.302397466827503 68-69 1.2854342581423401 70-71 1.373642943305187 72-73 1.3838208685162845 74-75 1.381559107358263 76-77 1.3819360675512666 78-79 1.2228588661037396 80-81 1.212303980699638 82-83 1.283549457177322 84-85 1.2729945717732207 86-87 1.2729945717732207 88-89 1.2827955367913149 90-91 1.2873190591073582 92-93 1.28958082026538 94-95 1.2839264173703255 96-97 1.2884499396863691 98-99 1.2982509047044632 100-101 1.2978739445114595 102-103 1.2790259348612787 104-105 1.2741254523522316 106-107 1.2733715319662244 108-109 1.2782720144752715 110-111 1.286565138721351 112-113 1.2797798552472859 114-115 1.2797798552472859 116-117 1.2978739445114595 118-119 1.2884499396863691 120-121 1.2794028950542822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 132640.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.104041013269 #Duplication Level Percentage of deduplicated Percentage of total 1 94.08828774579058 61.25527744270205 2 3.618824837297635 4.712002412545235 3 0.9356833499316766 1.827503015681544 4 0.40994047756907614 1.0675512665862485 5 0.23623688537878965 0.7689987937273824 6 0.128540658220812 0.5021109770808203 7 0.09379993978275471 0.42747285886610376 8 0.06253329318850315 0.32569360675512665 9 0.0509530537091507 0.2985524728588661 >10 0.28834796303587557 3.5404101326899884 >50 0.031266646594251574 1.38645958986731 >100 0.037056766333927786 5.762967430639324 >500 0.011580239479352434 4.8582629674306395 >1k 0.005790119739676217 9.272466827503017 >5k 0.0011580239479352433 3.994270205066345 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5298 3.994270205066345 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3503 2.6409831121833536 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2855 2.152442702050663 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2683 2.0227683956574185 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1913 1.4422496984318456 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1345 1.0140229191797345 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 782 0.5895657418576599 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 730 0.5503618817852834 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 725 0.5465922798552473 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 696 0.5247285886610373 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 639 0.48175512665862485 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 635 0.4787394451145959 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 623 0.4696924004825091 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 580 0.43727382388419783 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 523 0.39430036188178524 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 511 0.3852533172496984 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 479 0.36112786489746684 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 445 0.3354945717732208 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 405 0.30533775633293125 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 403 0.30382991556091676 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 394 0.2970446320868516 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 334 0.25180940892641734 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 326 0.24577804583835947 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 304 0.22919179734620024 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 273 0.20582026537997586 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 256 0.19300361881785283 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 243 0.18320265379975875 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 241 0.1816948130277443 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 239 0.18018697225572977 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 237 0.1786791314837153 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 219 0.16510856453558503 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 217 0.16360072376357057 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 210 0.1583232810615199 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 209 0.15756936067551267 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 208 0.15681544028950542 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 206 0.15530759951749096 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 199 0.1500301568154403 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 195 0.14701447527141134 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 186 0.1402291917973462 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 159 0.1198733413751508 No Hit ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 158 0.11911942098914355 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 144 0.10856453558504221 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 141 0.10630277442702052 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 7.539203860072376E-4 0.0 9 0.0 0.0 0.0 7.539203860072376E-4 0.0 10-11 0.0 0.0 0.0 0.0030156815440289505 0.0 12-13 0.0 0.0 0.0 0.004523522316043426 0.0 14-15 0.0 0.0 0.0 0.004523522316043426 0.0 16-17 0.0 0.0 0.0 0.008670084439083233 0.0 18-19 0.0 0.0 0.0 0.016586248492159228 0.0 20-21 0.0 0.0 0.0 0.021486731001206273 0.0 22-23 0.0 0.0 0.0 0.030156815440289503 0.0 24-25 0.0 0.0 0.0 0.035811218335343786 0.0 26-27 0.0 0.0 0.0 0.04485826296743064 0.0 28-29 0.0 0.0 0.0 0.0738841978287093 0.0 30-31 0.0 0.0 0.0 0.12138118214716526 0.0 32-33 0.0 0.0 0.0 0.19752714113389624 0.0 34-35 0.0 0.0 0.0 0.2691495778045838 0.0 36-37 0.0 0.0 0.0 0.33511761158021713 0.0 38-39 0.0 0.0 0.0 0.42784981905910735 0.0 40-41 0.0 0.0 0.0 0.5454613992762365 0.0 42-43 0.0 0.0 0.0 0.7128317249698432 0.0 44-45 0.0 0.0 0.0 0.8134800965018094 0.0 46-47 0.0 0.0 0.0 1.0777291917973462 0.0 48-49 0.0 0.0 0.0 1.2963661037394452 0.0 50-51 0.0 0.0 0.0 1.3570566948130278 0.0 52-53 0.0 0.0 0.0 1.4539354644149578 0.0 54-55 0.0 0.0 0.0 1.714791917973462 0.0 56-57 0.0 0.0 0.0 2.104568757539204 0.0 58-59 0.0 0.0 0.0 2.4034981905910735 0.0 60-61 0.0 0.0 0.0 2.707705066344994 0.0 62-63 0.0 0.0 0.0 3.0481001206272618 0.0 64-65 0.0 0.0 0.0 3.3805790108564535 0.0 66-67 0.0 0.0 0.0 4.171064535585042 0.0 68-69 0.0 0.0 0.0 5.010554885404101 0.0 70-71 0.0 0.0 0.0 5.252186369119421 0.0 72-73 0.0 0.0 0.0 5.6027593486127865 0.0 74-75 0.0 0.0 0.0 5.98537394451146 0.0 76-77 0.0 0.0 0.0 6.281664656212304 0.0 78-79 0.0 0.0 0.0 6.667294933655006 0.0 80-81 0.0 0.0 0.0 7.53844993968637 0.0 82-83 0.0 0.0 0.0 8.71984318455971 0.0 84-85 0.0 0.0 0.0 11.218712303980698 0.0 86-87 0.0 0.0 0.0 13.15101025331725 0.0 88-89 0.0 0.0 0.0 15.000753920386007 0.0 90-91 0.0 0.0 0.0 15.800286489746682 0.0 92-93 0.0 0.0 0.0 16.695566948130278 0.0 94-95 0.0 0.0 0.0 17.58556996381182 0.0 96-97 0.0 3.769601930036188E-4 0.0 18.50723763570567 0.0 98-99 0.0 7.539203860072376E-4 0.0 18.97617611580217 0.0 100-101 0.0 7.539203860072376E-4 0.0 19.09152593486128 0.0 102-103 0.0 7.539203860072376E-4 0.0 19.266812424607963 0.0 104-105 0.0 7.539203860072376E-4 0.0 19.495250301568156 0.0 106-107 0.0 7.539203860072376E-4 0.0 19.848838962605548 0.0 108-109 0.0 7.539203860072376E-4 0.0 20.548477080820263 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 25 0.0023339782 69.12551 2 TACCTGG 85 1.8189894E-11 60.993107 2 CTAGAAG 50 9.127551E-6 57.450512 3 GACCCCG 35 0.00893709 49.2433 7 AGTACGC 50 6.2347186E-4 46.083675 2 GAGTACG 55 9.950207E-4 41.91031 1 ACCTGGG 130 1.2732926E-9 39.773434 3 GACCTAG 30 0.0015349044 38.388355 44-45 TACGCAG 60 0.0015494811 38.300343 4 GTACGCA 60 0.0015494811 38.300343 3 TATATTG 65 0.0022911115 35.367676 5 TCTCGGG 110 0.0 33.973995 114-115 TGGGGGT 85 2.0868557E-4 33.807343 6 CTGGGGG 90 2.9188485E-4 31.929155 5 GTACCTG 200 2.577508E-9 28.81334 1 GCCGCGC 40 0.0063196076 28.758224 66-67 TGTACAT 100 5.4114446E-4 28.736238 5 GACACAG 80 0.006343678 28.72526 7 GCGCTTC 40 0.0063670008 28.714287 30-31 GAGTACT 345 0.0 28.330599 12-13 >>END_MODULE