##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003790_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 372590 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89058482514292 31.0 31.0 34.0 28.0 34.0 2 30.677833543573364 31.0 31.0 34.0 27.0 34.0 3 30.863844977052523 33.0 31.0 34.0 27.0 34.0 4 34.27268847795163 37.0 35.0 37.0 30.0 37.0 5 34.18008803242169 37.0 35.0 37.0 30.0 37.0 6 34.28653479696181 37.0 35.0 37.0 30.0 37.0 7 34.23984272256367 37.0 35.0 37.0 30.0 37.0 8 34.32098016586597 37.0 35.0 37.0 30.0 37.0 9 35.81553718564642 39.0 37.0 39.0 30.0 39.0 10-11 35.730432915537186 39.0 36.0 39.0 30.0 39.0 12-13 35.71574518908183 39.0 36.0 39.0 30.0 39.0 14-15 36.73609463485332 40.0 37.0 41.0 30.5 41.0 16-17 36.68726213800692 40.0 37.0 41.0 30.0 41.0 18-19 36.596038541023646 40.0 36.5 41.0 30.0 41.0 20-21 36.5475415335892 40.0 36.5 41.0 29.5 41.0 22-23 36.342664859497035 39.5 36.0 41.0 29.0 41.0 24-25 36.265428487076946 39.0 36.0 41.0 29.0 41.0 26-27 36.13786065111785 39.0 36.0 41.0 28.0 41.0 28-29 35.978766204138594 39.0 36.0 41.0 27.0 41.0 30-31 35.66757964518639 39.0 35.0 40.0 27.0 41.0 32-33 35.41406774202206 38.5 35.0 40.0 26.5 41.0 34-35 35.23853968168764 38.0 35.0 40.0 25.0 41.0 36-37 35.23458090662659 38.5 35.0 40.0 25.0 41.0 38-39 35.15785582007032 38.5 35.0 40.0 25.0 41.0 40-41 35.27312864006012 39.0 35.0 41.0 25.0 41.0 42-43 35.1303604498242 39.0 35.0 41.0 24.0 41.0 44-45 34.8815306368931 39.0 35.0 41.0 23.5 41.0 46-47 34.53585308247672 39.0 34.0 40.0 22.0 41.0 48-49 34.22244155774443 38.5 34.0 40.0 19.0 41.0 50-51 33.449504817627954 37.5 33.5 39.5 15.0 40.5 52-53 33.5775705198744 38.0 33.0 40.0 15.5 40.5 54-55 33.89447516036394 38.0 34.0 40.0 16.0 41.0 56-57 33.55576370809737 38.0 33.0 40.0 11.0 41.0 58-59 33.30737003140181 38.0 33.0 40.0 8.5 41.0 60-61 32.83176950535441 38.0 32.0 40.0 8.0 41.0 62-63 32.69174293459298 37.0 32.0 40.0 7.5 41.0 64-65 32.36184546015728 37.0 32.0 39.5 4.5 41.0 66-67 31.94063984540648 36.0 31.0 39.0 2.0 41.0 68-69 31.555946214337474 36.0 31.0 39.0 2.0 41.0 70-71 31.02190745860061 35.0 30.0 38.5 2.0 40.0 72-73 30.633289943369387 35.0 30.0 37.5 2.0 40.0 74-75 30.203519954910224 35.0 29.5 37.0 2.0 39.5 76-77 29.769627472556966 35.0 29.0 36.5 2.0 39.0 78-79 29.150165060790684 34.0 29.0 36.0 2.0 39.0 80-81 28.716071284790253 34.0 28.0 36.0 2.0 37.5 82-83 28.320654070157545 34.0 26.5 35.0 2.0 37.0 84-85 28.077974449126387 34.0 26.5 35.0 2.0 37.0 86-87 27.93534850640114 34.0 27.0 35.0 2.0 36.0 88-89 27.57975925279798 34.0 26.0 35.0 2.0 36.0 90-91 27.28418100324754 34.0 25.5 35.0 2.0 36.0 92-93 26.99910357229126 34.0 25.0 35.0 2.0 35.0 94-95 26.739331436699857 34.0 25.0 35.0 2.0 35.0 96-97 26.509358812635874 33.5 25.0 35.0 2.0 35.0 98-99 26.187272873668107 33.0 24.0 35.0 2.0 35.0 100-101 25.81870823156821 33.0 21.0 35.0 2.0 35.0 102-103 25.48393408304034 32.5 19.5 35.0 2.0 35.0 104-105 25.06018411658928 32.0 18.5 35.0 2.0 35.0 106-107 24.61638798679514 32.0 16.0 35.0 2.0 35.0 108-109 24.341969725435465 32.0 8.5 34.5 2.0 35.0 110-111 23.964528033495263 31.5 4.5 34.0 2.0 35.0 112-113 23.366795941920074 31.0 2.0 34.0 2.0 35.0 114-115 22.80218202313535 31.0 2.0 34.0 2.0 35.0 116-117 22.073598325236855 31.0 2.0 34.0 2.0 35.0 118-119 21.237011191926783 29.5 2.0 34.0 2.0 35.0 120-121 19.604201669395316 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.67615464073846 1101 2 0.9771387922225863 1101 3 0.9110370383256985 1101 4 1.267164526295737 1101 5 1.2781738428590828 1101 6 1.2507291313630375 1101 7 1.2053919021102502 1101 8 1.1432619844719767 1101 9 1.1074012844015684 1101 10-11 1.1513040721674912 1101 12-13 0.7886238970449995 1101 14-15 1.2519183737951067 1101 16-17 1.005971719198648 1101 18-19 1.290031776934363 1101 20-21 1.15737635798029 1101 22-23 1.4420193558148071 1101 24-25 0.9178927652898139 1101 26-27 1.1011406054406336 1101 28-29 0.878930332280973 1101 30-31 0.8685155114325838 1101 32-33 0.9401250446103617 1101 34-35 0.904481955611935 1101 36-37 0.6869103648503554 1101 38-39 1.0162024754235404 1101 40-41 1.4618962080059106 1101 42-43 1.6272146000097436 1101 44-45 1.0612078649471854 1101 46-47 1.180661010846265 1101 48-49 1.5943546156873936 1101 50-51 1.1925031870915603 1101 52-53 1.1693960487530362 1101 54-55 1.0939454926109704 1101 56-57 1.1691468946111172 1101 58-59 0.9867397060972039 1101 60-61 0.9792088950549811 1101 62-63 1.1389601448970907 1101 64-65 0.7374998085082964 1101 66-67 0.9249249206339307 1101 68-69 1.1747261499793247 1101 70-71 0.9371570968305711 1101 72-73 0.7016377014092257 1101 74-75 0.9177721419454024 1101 76-77 1.1689693419076974 1101 78-79 1.111186724726263 1101 80-81 0.9046670574283446 1101 82-83 1.145475928558973 1101 84-85 0.7531306659387411 1101 86-87 0.8017380373364453 1101 88-89 0.27440304414435346 1101 90-91 0.617232852312231 1101 92-93 0.4593400235704088 1101 94-95 0.3113817947233777 1101 96-97 0.04319333794012081 1101 98-99 0.3570785848809557 1101 100-101 0.7516659234809104 1101 102-103 0.46511812130214025 1101 104-105 0.025280198765347706 1101 106-107 -0.15392422646776893 1101 108-109 0.16825188469915275 1101 110-111 -0.08446975699291315 1101 112-113 -0.08641395734637669 1101 114-115 -0.14711858201863848 1101 116-117 -0.9533472669164027 1101 118-119 -1.5650807320514772 1101 120-121 -1.3568316019412379 1102 1 0.41208360248709397 1102 2 0.6411187557927143 1102 3 0.56299514397233 1102 4 0.7650242712866344 1102 5 0.7997130049720198 1102 6 0.9988621003976448 1102 7 0.8180512828024149 1102 8 0.4566954999546766 1102 9 0.8484759656401835 1102 10-11 0.7719324874680353 1102 12-13 0.6600492158063815 1102 14-15 0.9078609967459244 1102 16-17 0.6886902984336274 1102 18-19 0.9726820501584044 1102 20-21 1.053915520093227 1102 22-23 1.2366687182920444 1102 24-25 0.9801650785867224 1102 26-27 0.9872744852220521 1102 28-29 1.0890623905687704 1102 30-31 1.1610929249116353 1102 32-33 0.8745512741185522 1102 34-35 1.0014764911310579 1102 36-37 1.288618015123582 1102 38-39 1.3757425482832843 1102 40-41 1.295502765382956 1102 42-43 1.043676348643622 1102 44-45 1.1627561345282444 1102 46-47 0.8385617394437048 1102 48-49 0.9099511548495087 1102 50-51 0.5801853364540364 1102 52-53 0.981304063324977 1102 54-55 0.8240001374197163 1102 56-57 0.5260230330446305 1102 58-59 0.7509018190298136 1102 60-61 0.8399101701005236 1102 62-63 0.33033081884973114 1102 64-65 0.33112458082158724 1102 66-67 0.603021459796043 1102 68-69 0.7982461864092052 1102 70-71 0.4751352389070753 1102 72-73 0.24719781069884306 1102 74-75 0.551583617355238 1102 76-77 0.6766879211426691 1102 78-79 0.49064938410695547 1102 80-81 0.21976268584364433 1102 82-83 0.46125461708356497 1102 84-85 3.301194906519811E-4 1102 86-87 -0.06415449453969657 1102 88-89 0.5030460313938967 1102 90-91 0.7137037084142328 1102 92-93 0.8015986756651294 1102 94-95 0.7477433612078954 1102 96-97 0.10874433976161768 1102 98-99 -0.023727426048008482 1102 100-101 0.007654083772347775 1102 102-103 0.13720373150250254 1102 104-105 -0.05459229668091581 1102 106-107 -0.2233003649012808 1102 108-109 0.1168402271399529 1102 110-111 0.10248379491965665 1102 112-113 0.1485814889195609 1102 114-115 -0.14497832700952884 1102 116-117 -0.10380264032259134 1102 118-119 -0.7792656136543918 1102 120-121 -0.7970866110487087 1103 1 -0.10910408079736555 1103 2 0.21360979521923795 1103 3 0.20264160861879432 1103 4 0.6228525541149139 1103 5 0.6691085735611324 1103 6 0.6310794350343301 1103 7 0.3446560400523353 1103 8 0.46481703791491924 1103 9 0.48260760461052854 1103 10-11 0.42239680809364444 1103 12-13 0.5634664526913582 1103 14-15 0.3235134894145375 1103 16-17 0.06362920361446811 1103 18-19 0.15432916648293826 1103 20-21 0.32617032621899966 1103 22-23 0.4959510578163133 1103 24-25 0.42275631351665766 1103 26-27 0.7481127061409225 1103 28-29 0.5029227685420494 1103 30-31 0.48933502331502154 1103 32-33 0.7193704334538396 1103 34-35 0.3741548880030052 1103 36-37 0.208107262435405 1103 38-39 0.1869554840929908 1103 40-41 0.32526816127737845 1103 42-43 0.24972473574039356 1103 44-45 0.17554531667226314 1103 46-47 0.3212311757421844 1103 48-49 0.48309794533501105 1103 50-51 0.19012750002522694 1103 52-53 0.2506140991672723 1103 54-55 -0.1682774774385436 1103 56-57 -0.06429140162463653 1103 58-59 -0.1452451343752088 1103 60-61 0.17877462106174846 1103 62-63 0.03844013784614475 1103 64-65 0.043033997569715154 1103 66-67 -0.13061327464513894 1103 68-69 -0.17904690580324356 1103 70-71 -0.6115705284438526 1103 72-73 -0.3315778491252068 1103 74-75 -0.08525655859784464 1103 76-77 0.014261234925662336 1103 78-79 -0.1734350082032421 1103 80-81 -0.5775613610771764 1103 82-83 -0.187609019280071 1103 84-85 -0.08690106330504577 1103 86-87 -0.017681702099160646 1103 88-89 -0.11182037460154248 1103 90-91 -0.389982345675044 1103 92-93 0.01228619603007175 1103 94-95 -0.28062499982175737 1103 96-97 -0.47979834515203734 1103 98-99 -0.6447562628026482 1103 100-101 -0.3160792169770801 1103 102-103 -0.46754537244014927 1103 104-105 -1.0545597127773654 1103 106-107 -0.9830470250511674 1103 108-109 -1.268309658816385 1103 110-111 -0.9473329206228662 1103 112-113 -1.0517321311521215 1103 114-115 -1.2471858864751546 1103 116-117 -0.9587716856142201 1103 118-119 -0.6489829482910814 1103 120-121 -0.6135740662458424 1104 1 0.6147363356060644 1104 2 0.62279971327753 1104 3 0.8723288345761162 1104 4 0.7444544249129237 1104 5 0.950454341163514 1104 6 0.943136841376564 1104 7 0.907243851576979 1104 8 0.7407880330021896 1104 9 0.907657816301132 1104 10-11 1.0373933663533563 1104 12-13 0.9294177402794048 1104 14-15 0.9918240192539542 1104 16-17 0.9177229886801399 1104 18-19 0.7498256728808101 1104 20-21 0.6375346697895523 1104 22-23 0.9846546061091175 1104 24-25 0.6518112022022962 1104 26-27 0.3572771647504567 1104 28-29 0.37416867852876123 1104 30-31 0.8519200060120298 1104 32-33 0.5661375549331353 1104 34-35 0.895635119212514 1104 36-37 0.721643202690565 1104 38-39 0.9949770963361573 1104 40-41 1.4390718972157543 1104 42-43 1.2635718470359123 1104 44-45 1.1006803931920501 1104 46-47 0.9544736722768619 1104 48-49 1.1783907761428267 1104 50-51 0.8983480731457121 1104 52-53 1.0347651208455275 1104 54-55 1.2585124632503693 1104 56-57 1.3018626186109046 1104 58-59 1.1651479783724312 1104 60-61 0.9480648682069841 1104 62-63 1.0752620442873848 1104 64-65 1.0211388716397423 1104 66-67 0.7260028817324589 1104 68-69 0.8039848430722962 1104 70-71 0.6662392046091092 1104 72-73 0.6845370389946623 1104 74-75 0.8722025884163322 1104 76-77 0.8784162169626057 1104 78-79 0.7381216956467895 1104 80-81 0.5886935540108453 1104 82-83 0.4969861283375856 1104 84-85 1.0891698836921542 1104 86-87 0.7212599391866341 1104 88-89 0.868792726642198 1104 90-91 0.4715323972316696 1104 92-93 0.40184876498274136 1104 94-95 0.6492483629942463 1104 96-97 1.027603753838072 1104 98-99 0.6124596050345232 1104 100-101 0.5914652663375541 1104 102-103 0.4336578222491987 1104 104-105 0.2648432160021841 1104 106-107 0.4309377558561316 1104 108-109 -0.046887890044754243 1104 110-111 0.09167189781354423 1104 112-113 0.6798828465472866 1104 114-115 0.6188926983566709 1104 116-117 0.4962688137681539 1104 118-119 0.6209532144985204 1104 120-121 0.6158108523310695 1105 1 0.7173866327901237 1105 2 0.9906137052876645 1105 3 0.9109749419521265 1105 4 1.1304283116906717 1105 5 1.0199150251740363 1105 6 1.0483570498925943 1105 7 1.1920411817923195 1105 8 1.0645237827829561 1105 9 1.3361022282664408 1105 10-11 1.32647794201349 1105 12-13 1.0921199228770888 1105 14-15 1.0828609967459215 1105 16-17 1.5504327226760495 1105 18-19 1.4484396259159809 1105 20-21 0.7305569342346416 1105 22-23 1.0490424556657771 1105 24-25 1.3993569977786393 1105 26-27 1.0846229700705337 1105 28-29 1.4282038047101864 1105 30-31 1.4900070663257807 1105 32-33 1.8631876377549261 1105 34-35 1.6189007335553072 1105 36-37 1.0901331666387293 1105 38-39 1.4475859826267197 1105 40-41 1.318608825989017 1105 42-43 1.523979378946656 1105 44-45 1.578160174932286 1105 46-47 1.394117294999262 1105 48-49 1.3176531750515323 1105 50-51 1.0338469526156473 1105 52-53 1.483324265345182 1105 54-55 1.701651652571229 1105 56-57 1.6713513158729185 1105 58-59 1.3472402028681856 1105 60-61 1.4166778468681969 1105 62-63 1.200280313799226 1105 64-65 1.0499377121347209 1105 66-67 1.294056813331398 1105 68-69 1.4169330550960701 1105 70-71 0.9444534207252566 1105 72-73 0.8903796288806625 1105 74-75 0.9496896779612989 1105 76-77 0.906612163566912 1105 78-79 1.0044120103695846 1105 80-81 0.894762685843645 1105 82-83 1.1078455261744757 1105 84-85 0.7431331497936817 1105 86-87 0.7780172226320197 1105 88-89 0.7717329000807638 1105 90-91 0.6869360316465567 1105 92-93 0.8082905948570485 1105 94-95 0.8550665935311272 1105 96-97 0.7067241377414142 1105 98-99 0.9137725739519915 1105 100-101 0.7976793362976018 1105 102-103 0.045284539583310135 1105 104-105 -0.07820340779202795 1105 106-107 -0.031128647729566694 1105 108-109 0.13173921703894464 1105 110-111 -0.16531923538337523 1105 112-113 0.001359266697338768 1105 114-115 -0.8134126704438742 1105 116-117 -0.5567066807266343 1105 118-119 -0.4639878358766154 1105 120-121 -0.4794098433719398 1106 1 0.2602543341944106 1106 2 0.8212678072832276 1106 3 0.4033067970888595 1106 4 0.7370432414763357 1106 5 0.6077753355953277 1106 6 0.41559651774181816 1106 7 0.7629022313119052 1106 8 0.5852185351850281 1106 9 0.8583675645561684 1106 10-11 0.5335178533216904 1106 12-13 0.36082834859770685 1106 14-15 0.6941414845507978 1106 16-17 0.7745642821734577 1106 18-19 0.6074381477193853 1106 20-21 0.48088028974091657 1106 22-23 0.7671226478313358 1106 24-25 0.8522856747926824 1106 26-27 0.8131891193683956 1106 28-29 0.7771043959888289 1106 30-31 0.7650902148845304 1106 32-33 0.9416244448502695 1106 34-35 1.2921268976351286 1106 36-37 1.102744031383743 1106 38-39 1.0454241200989998 1106 40-41 0.6751775621309193 1106 42-43 0.5744690315704517 1106 44-45 0.7162791697586002 1106 46-47 0.8326335551618698 1106 48-49 1.3455541358793255 1106 50-51 1.1653140627413023 1106 52-53 0.9833704589889365 1106 54-55 1.4065204626229644 1106 56-57 1.431375336567676 1106 58-59 1.2000210602222197 1106 60-61 1.5485145061438814 1106 62-63 0.9209473500150409 1106 64-65 1.0529402989787755 1106 66-67 0.8021407009884634 1106 68-69 1.0643369723170615 1106 70-71 0.9572490600452852 1106 72-73 0.7993319570403052 1106 74-75 1.1736364628836924 1106 76-77 1.281057839841857 1106 78-79 0.7783865114782316 1106 80-81 0.6798236614534012 1106 82-83 0.5422505520429155 1106 84-85 0.6386431276207354 1106 86-87 0.781712714132393 1106 88-89 0.4602953538871226 1106 90-91 0.4692253886310347 1106 92-93 0.34252008487922225 1106 94-95 0.6093626024003065 1106 96-97 0.4556616839350589 1106 98-99 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1.4464081916950313 2204 80-81 1.5578724419412069 2204 82-83 1.4169117986553807 2204 84-85 1.29379217911125 2204 86-87 1.1719566165714141 2204 88-89 1.6472872238058223 2204 90-91 1.680607502452176 2204 92-93 1.4441461011393848 2204 94-95 1.5707447162214443 2204 96-97 1.6995641229594476 2204 98-99 1.8341655278815345 2204 100-101 1.7392597748292609 2204 102-103 1.7510926203913897 2204 104-105 1.680096050202554 2204 106-107 1.5242267353697194 2204 108-109 1.7766640753784344 2204 110-111 1.7694553396351047 2204 112-113 2.065214280247474 2204 114-115 1.7488563613373493 2204 116-117 1.6620510182139903 2204 118-119 1.5732750367521149 2204 120-121 1.3044039038564392 2205 1 0.83323744864094 2205 2 1.0870589267328867 2205 3 1.1320122379894215 2205 4 1.0763490576114165 2205 5 1.1204395006985166 2205 6 1.0185202200557626 2205 7 1.0842905990417364 2205 8 1.1564818247410003 2205 9 1.1961255382897562 2205 10-11 1.1972530002885478 2205 12-13 1.1043868226439884 2205 14-15 1.033822535207463 2205 16-17 1.1547159394592654 2205 18-19 1.3473615373378962 2205 20-21 1.4688727850504932 2205 22-23 1.39542940205272 2205 24-25 1.313284736706379 2205 26-27 1.0933642288118008 2205 28-29 1.00882734783373 2205 30-31 1.180537369356081 2205 32-33 1.379679479246768 2205 34-35 1.1748739270285 2205 36-37 1.1216308356364024 2205 38-39 1.3502083602490984 2205 40-41 1.2676181499983414 2205 42-43 1.296560964028238 2205 44-45 1.3304911772632835 2205 46-47 1.365591653973624 2205 48-49 1.40343406083241 2205 50-51 1.382215250983947 2205 52-53 1.5744664564873787 2205 54-55 1.2791283425479207 2205 56-57 1.3280529475745553 2205 58-59 1.539897545525534 2205 60-61 1.496747776938129 2205 62-63 1.2621684256873422 2205 64-65 1.499821162018172 2205 66-67 1.553235135009718 2205 68-69 1.5013444770074962 2205 70-71 1.524494213266049 2205 72-73 1.5683516568526876 2205 74-75 1.6996605404321592 2205 76-77 1.5497648442195917 2205 78-79 1.561696392653964 2205 80-81 1.5591857627667238 2205 82-83 1.3593315401604897 2205 84-85 1.349048068208603 2205 86-87 1.5405463601611586 2205 88-89 1.4917212450691082 2205 90-91 0.8504558451663726 2205 92-93 0.3667696158360698 2205 94-95 0.9646237030882396 2205 96-97 1.2248768183940975 2205 98-99 1.2971059072853244 2205 100-101 1.6084019470202158 2205 102-103 1.6047250990238702 2205 104-105 1.4602581306695122 2205 106-107 1.4195415154405957 2205 108-109 1.3241051844049139 2205 110-111 1.0678684103042713 2205 112-113 1.0901413179793913 2205 114-115 1.4568379123067068 2205 116-117 1.47141103489108 2205 118-119 1.4231916513028722 2205 120-121 1.1289817650196667 2206 1 0.7111993581141007 2206 2 1.1887161119913578 2206 3 1.0732934648011963 2206 4 0.9325573188036032 2206 5 1.109618328301778 2206 6 1.1682172272286522 2206 7 1.2818419223165307 2206 8 1.1636176149957578 2206 9 0.9349890060117403 2206 10-11 1.1161715014015812 2206 12-13 1.2766846213083483 2206 14-15 1.2321777170031538 2206 16-17 1.2489126992910684 2206 18-19 1.3720121680576582 2206 20-21 1.321618271075721 2206 22-23 1.2820822588422374 2206 24-25 1.370460719887184 2206 26-27 1.4223897049433774 2206 28-29 1.3275448843165378 2206 30-31 1.3591547327001265 2206 32-33 1.4511857864443911 2206 34-35 1.5692685597269929 2206 36-37 1.6476882187677333 2206 38-39 1.376809893763813 2206 40-41 1.3739593570228266 2206 42-43 1.2362406573252969 2206 44-45 1.307272033063434 2206 46-47 1.4046344439810525 2206 48-49 1.2523457600656585 2206 50-51 1.6997950184833286 2206 52-53 1.467241005089896 2206 54-55 1.3437214664658086 2206 56-57 1.5398047407974005 2206 58-59 1.8243119655106952 2206 60-61 1.8307015241555433 2206 62-63 1.407255613419217 2206 64-65 1.4753189359484224 2206 66-67 1.8240736212822917 2206 68-69 1.6858176404999519 2206 70-71 1.5472681435158613 2206 72-73 1.2769807688981984 2206 74-75 1.3971222025642405 2206 76-77 1.351888885772894 2206 78-79 1.1247776441998454 2206 80-81 1.1939990202487891 2206 82-83 1.11434947238903 2206 84-85 1.085954449608554 2206 86-87 1.1593628330773136 2206 88-89 1.167828096417118 2206 90-91 0.860599921347422 2206 92-93 0.804581883953805 2206 94-95 1.0616277842161104 2206 96-97 1.0718159724875882 2206 98-99 1.099597868700119 2206 100-101 1.1123655878809622 2206 102-103 0.9183845103520909 2206 104-105 1.0003737626259799 2206 106-107 1.0097158909043635 2206 108-109 1.2859095376079779 2206 110-111 1.0796140200000401 2206 112-113 0.8398506922064506 2206 114-115 0.47660974015731483 2206 116-117 0.2760115790414659 2206 118-119 0.6217570730194204 2206 120-121 1.0426597269291413 2207 1 0.504374155071762 2207 2 0.9583053273910522 2207 3 1.1669909847328768 2207 4 1.382210842884966 2207 5 1.3833285724111164 2207 6 1.4295959090726313 2207 7 1.5536721978350982 2207 8 1.3381423211073766 2207 9 1.4236690731862325 2207 10-11 1.0802658208013725 2207 12-13 0.9910726858004395 2207 14-15 1.4515107293662481 2207 16-17 1.2438373572571493 2207 18-19 1.43573909115662 2207 20-21 1.3422993584770637 2207 22-23 1.337478284542783 2207 24-25 1.3994684059782827 2207 26-27 1.3729473907479033 2207 28-29 1.5325932877761375 2207 30-31 1.5375783675735448 2207 32-33 1.4516457482718508 2207 34-35 1.5375727478155596 2207 36-37 1.4855208671734843 2207 38-39 1.9228533623058581 2207 40-41 1.8340945657394627 2207 42-43 1.828012355773744 2207 44-45 2.0913063424902134 2207 46-47 2.016332090008177 2207 48-49 2.3876175244276396 2207 50-51 2.0964583608152907 2207 52-53 2.217838525594736 2207 54-55 2.073197998382284 2207 56-57 2.072532242789137 2207 58-59 2.0100075825473382 2207 60-61 1.9200200928574986 2207 62-63 1.9793578539061727 2207 64-65 1.9359671238994238 2207 66-67 1.9918063676986044 2207 68-69 1.5910640711388488 2207 70-71 1.841623652454306 2207 72-73 2.009029361500982 2207 74-75 2.099867931080727 2207 76-77 2.2325925557237767 2207 78-79 2.2823754684800974 2207 80-81 2.339838443419403 2207 82-83 2.459449804249342 2207 84-85 2.3378301194906506 2207 86-87 2.0596571513307715 2207 88-89 2.17970972717524 2207 90-91 2.0473415574932616 2207 92-93 2.4256034290816473 2207 94-95 2.0312029571674906 2207 96-97 2.2928426760658347 2207 98-99 2.3680952120981615 2207 100-101 2.6138558068858373 2207 102-103 2.568680261508444 2207 104-105 2.7530131697480797 2207 106-107 2.5897492488836242 2207 108-109 2.8808479514417975 2207 110-111 2.9746094633688536 2207 112-113 2.2086899262338804 2207 114-115 1.9472825167219021 2207 116-117 1.5650624814837037 2207 118-119 1.6193454974567203 2207 120-121 1.5834243812269904 2208 1 0.15935396643856592 2208 2 1.002003600776348 2208 3 0.8897378745712103 2208 4 1.2473995842078693 2208 5 1.3255121576775863 2208 6 1.1036522024581075 2208 7 1.3253784539944036 2208 8 1.069486757416641 2208 9 1.3945090871499062 2208 10-11 1.2844830362837456 2208 12-13 1.2195981278033017 2208 14-15 1.4456209620838791 2208 16-17 1.5912394029974593 2208 18-19 1.4189703228331538 2208 20-21 1.3715257646532066 2208 22-23 1.4781067117447648 2208 24-25 1.2877064804738723 2208 26-27 1.4653146354242494 2208 28-29 1.330654437555097 2208 30-31 1.1919758442261 2208 32-33 1.2305593619290747 2208 34-35 1.3002922045914858 2208 36-37 1.4021867615129437 2208 38-39 1.6718454753004224 2208 40-41 1.753994966422816 2208 42-43 1.459122622473778 2208 44-45 1.2574509159647889 2208 46-47 1.1631453134856855 2208 48-49 1.450301145221161 2208 50-51 1.1773858948980944 2208 52-53 1.637179761380061 2208 54-55 1.5159700969806664 2208 56-57 1.6013215117659882 2208 58-59 1.4017442992916997 2208 60-61 1.7877389781113067 2208 62-63 1.6494984483509825 2208 64-65 1.7356980451004276 2208 66-67 1.5529685038938688 2208 68-69 1.4193587417721893 2208 70-71 1.6360826681387302 2208 72-73 1.6000357656381858 2208 74-75 1.5160333573898974 2208 76-77 1.6345085450594823 2208 78-79 1.5596316679505904 2208 80-81 1.8370287170394874 2208 82-83 1.7234513241892842 2208 84-85 1.742220645198337 2208 86-87 1.8108936471895554 2208 88-89 2.08037609108964 2208 90-91 1.7764107178500126 2208 92-93 2.0067209556574284 2208 94-95 2.2257305555079157 2208 96-97 2.1450841240866687 2208 98-99 2.3950695121380114 2208 100-101 2.182815358565339 2208 102-103 1.8361109142485077 2208 104-105 1.6554404396949742 2208 106-107 1.5678584065217862 2208 108-109 1.7343204890309636 2208 110-111 1.697739427501979 2208 112-113 1.7300926578007463 2208 114-115 2.281369641409494 2208 116-117 2.4191513361842425 2208 118-119 1.983296970593635 2208 120-121 1.5587577948823537 2209 1 0.6920537814135344 2209 2 1.2708790827200396 2209 3 0.9178051726889187 2209 4 1.1301722721702205 2209 5 1.4589851498803483 2209 6 1.2583772318312612 2209 7 1.3772405913953065 2209 8 1.3978872384128138 2209 9 1.1465652079640165 2209 10-11 1.18198881616253 2209 12-13 1.177226706418267 2209 14-15 1.2263749132469144 2209 16-17 1.0191657788842576 2209 18-19 1.03429570158319 2209 20-21 0.7750663926357433 2209 22-23 1.02233527451029 2209 24-25 1.0548144048513564 2209 26-27 0.7659441339960793 2209 28-29 1.0345919907454828 2209 30-31 1.0660244468908715 2209 32-33 1.1957905053975466 2209 34-35 1.250151110084012 2209 36-37 1.0629139064423327 2209 38-39 1.1653024113272465 2209 40-41 1.3043496838720188 2209 42-43 1.2609477204130073 2209 44-45 1.3046271307729924 2209 46-47 1.1978366455625533 2209 48-49 1.241398581401313 2209 50-51 0.9925417535072683 2209 52-53 1.2319198132697622 2209 54-55 1.1355972215814205 2209 56-57 0.8462074819070153 2209 58-59 0.698146130936145 2209 60-61 1.0001542611100263 2209 62-63 1.0974089058830856 2209 64-65 1.2209081019172388 2209 66-67 1.4258456259104726 2209 68-69 1.3342661775733298 2209 70-71 1.2676468359912327 2209 72-73 1.248017890221707 2209 74-75 1.4164196014506665 2209 76-77 1.2509175434090736 2209 78-79 0.7773237821630659 2209 80-81 1.1269942961816177 2209 82-83 1.1694305614175633 2209 84-85 1.30834039119377 2209 86-87 1.2471719909915606 2209 88-89 1.3745779509874474 2209 90-91 1.6179145544491362 2209 92-93 1.774052839570583 2209 94-95 1.4966526830545916 2209 96-97 1.5471776289155805 2209 98-99 1.4998395056948688 2209 100-101 2.1784929395267163 2209 102-103 1.5899483747541048 2209 104-105 1.4589486796831217 2209 106-107 1.5093067700666865 2209 108-109 1.7246213183703496 2209 110-111 1.5965941328918234 2209 112-113 2.0153171858490957 2209 114-115 1.9524316575276934 2209 116-117 1.5090314661496826 2209 118-119 1.3777948016316728 2209 120-121 1.0064477384100954 2210 1 0.40565226786426933 2210 2 0.7618097078666466 2210 3 0.8099917053305923 2210 4 0.8549608713296166 2210 5 1.1212206344583748 2210 6 0.7911842581453357 2210 7 0.9400263526498307 2210 8 0.7328023127636172 2210 9 0.7970145429021613 2210 10-11 0.725978264386157 2210 12-13 0.8395006449918512 2210 14-15 0.6903319832062706 2210 16-17 0.9972412333143481 2210 18-19 0.9552900450004458 2210 20-21 0.941912511281707 2210 22-23 1.1210524492183254 2210 24-25 0.8860994220210614 2210 26-27 0.34622838593127625 2210 28-29 0.7563388788558996 2210 30-31 0.7871137716771628 2210 32-33 0.9063049814041761 2210 34-35 0.7663056336197798 2210 36-37 0.7301637920417008 2210 38-39 1.0919283423946098 2210 40-41 0.8120404829845 2210 42-43 0.8172982055101627 2210 44-45 0.8500009228368626 2210 46-47 0.8495895709502648 2210 48-49 1.082804690203048 2210 50-51 1.010555527728485 2210 52-53 0.9513690751453154 2210 54-55 0.9260633224616299 2210 56-57 1.0687965125202439 2210 58-59 0.8463939746283486 2210 60-61 0.9734960286863839 2210 62-63 1.0451787664104444 2210 64-65 1.1727214000779824 2210 66-67 1.1948063297724083 2210 68-69 0.8987592962308248 2210 70-71 1.1189779210476267 2210 72-73 0.9403070950315318 2210 74-75 1.1448863252856043 2210 76-77 1.390251750929906 2210 78-79 1.0286302566622965 2210 80-81 0.7137907322653092 2210 82-83 0.8675167060584208 2210 84-85 1.0957282497292091 2210 86-87 1.051389240684891 2210 88-89 1.3133995667474352 2210 90-91 1.2055127563403047 2210 92-93 1.3751426258048234 2210 94-95 1.005735516806432 2210 96-97 0.786471075201117 2210 98-99 1.1201716713085368 2210 100-101 1.3915703743375794 2210 102-103 1.708969259119094 2210 104-105 1.4103120660957877 2210 106-107 1.4625447887630223 2210 108-109 1.4516940848661513 2210 110-111 1.5419663867958668 2210 112-113 1.7522860881028848 2210 114-115 1.7154396828765641 2210 116-117 2.0269087286866494 2210 118-119 2.2247613581917314 2210 120-121 2.526876423552622 2211 1 0.31571430788128296 2211 2 0.7579813652901777 2211 3 0.5342145999428212 2211 4 0.8167416525683251 2211 5 0.7472349044648752 2211 6 0.8710497444732539 2211 7 0.9603518821569565 2211 8 0.4202386073774491 2211 9 0.6456866618135351 2211 10-11 0.7611095275786823 2211 12-13 0.6625100272402236 2211 14-15 0.7314605818081645 2211 16-17 0.6887767438001475 2211 18-19 0.7635879975996218 2211 20-21 0.8422050544408464 2211 22-23 0.5144407330453831 2211 24-25 0.8724253367702133 2211 26-27 0.9002585792746132 2211 28-29 1.0268851199371483 2211 30-31 1.1299149627170735 2211 32-33 1.3171515507437306 2211 34-35 1.1525829918226265 2211 36-37 0.8887390386367215 2211 38-39 1.2916542587489346 2211 40-41 1.371851313100798 2211 42-43 1.1733962656560735 2211 44-45 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0.41357440304656023 2312 24-25 0.13181236540842178 2312 26-27 0.4027460614494416 2312 28-29 0.2832807368220074 2312 30-31 0.029632976591223326 2312 32-33 0.4049130312245026 2312 34-35 0.36129561257808973 2312 36-37 0.31442550866362495 2312 38-39 0.2984492666295324 2312 40-41 0.3033839023338558 2312 42-43 0.007080741408479696 2312 44-45 0.259009364502397 2312 46-47 -0.20488141301106566 2312 48-49 -0.03911215264403012 2312 50-51 -0.45731466354596506 2312 52-53 -0.4972812079928488 2312 54-55 -0.38891975922111044 2312 56-57 -0.7750751581698339 2312 58-59 -1.0290077416937038 2312 60-61 -9.060800424214726 2312 62-63 -2.3936678891606036 2312 64-65 -0.6545343339070939 2312 66-67 -0.3700405557078277 2312 68-69 -0.06528740532206356 2312 70-71 0.06609131125849643 2312 72-73 0.2044652525593058 2312 74-75 -0.09347452217964758 2312 76-77 -0.03187151038187608 2312 78-79 -0.17918265723671567 2312 80-81 -0.35313783095222107 2312 82-83 -0.27088362581049097 2312 84-85 0.10527197995576998 2312 86-87 0.14353284396159793 2312 88-89 0.25091424844816146 2312 90-91 0.265673992651962 2312 92-93 0.5452679263111229 2312 94-95 0.5075947823965272 2312 96-97 0.2729239263249248 2312 98-99 0.35595603648429375 2312 100-101 0.40077423881110974 2312 102-103 0.5469582534663253 2312 104-105 0.6981208816136579 2312 106-107 0.4828042862615085 2312 108-109 0.701917746519797 2312 110-111 1.0071026579687512 2312 112-113 0.7944794217360993 2312 114-115 0.8342852388819431 2312 116-117 1.3918045948195257 2312 118-119 1.1206374871208027 2312 120-121 1.2664792804241571 2313 1 0.08828842176420082 2313 2 -2.456036532025223 2313 3 -0.6895818305925978 2313 4 -2.0827334261698596 2313 5 -2.2912843176518116 2313 6 -0.18544178179780602 2313 7 0.7626965304597135 2313 8 0.5938775615343275 2313 9 0.545731655064543 2313 10-11 -0.07194301453999685 2313 12-13 0.3414581850165561 2313 14-15 0.43150557505917675 2313 16-17 0.5812103787549106 2313 18-19 0.7027021304796861 2313 20-21 -4.0909974651812036 2313 22-23 -1.817464481172486 2313 24-25 0.06890336506596384 2313 26-27 0.6345134410453497 2313 28-29 0.35152892336662944 2313 30-31 0.6721036344163238 2313 32-33 0.4731456516285988 2313 34-35 0.6470588204483292 2313 36-37 0.3575437179348242 2313 38-39 0.31508324440108737 2313 40-41 0.4418883075516291 2313 42-43 0.26701972213759717 2313 44-45 0.592006469200264 2313 46-47 0.7534044435936451 2313 48-49 0.5906907800168426 2313 50-51 0.06731719723047291 2313 52-53 0.36856979290998737 2313 54-55 0.2581366836044623 2313 56-57 0.44432959261624916 2313 58-59 0.5809888337553772 2313 60-61 0.5419516694311781 2313 62-63 0.2334265350478617 2313 64-65 0.6760375660960207 2313 66-67 0.7742731331561536 2313 68-69 0.881395450130757 2313 70-71 0.5128862429231411 2313 72-73 0.3732520275663127 2313 74-75 0.5860715691624669 2313 76-77 0.49701924644387674 2313 78-79 0.16812595706545608 2313 80-81 0.24370846897617326 2313 82-83 0.18128473756549113 2313 84-85 0.6121273082456717 2313 86-87 0.4229606326356752 2313 88-89 0.9092374637901486 2313 90-91 0.6715517673165081 2313 92-93 0.4101662794134562 2313 94-95 0.4849086891864758 2313 96-97 0.5924625459195809 2313 98-99 0.7474071121046038 2313 100-101 0.768480723665256 2313 102-103 0.6668891398023682 2313 104-105 0.9873086404007445 2313 106-107 1.5259131558483823 2313 108-109 0.9451200129498609 2313 110-111 1.0445018672088118 2313 112-113 1.1263090658941266 2313 114-115 0.8445464387200552 2313 116-117 0.9876297559290776 2313 118-119 0.3627189914326223 2313 120-121 0.7371838039445961 2314 1 -0.639422421609293 2314 2 -5.562060628410766 2314 3 -1.8654854450504281 2314 4 -0.34779366713371473 2314 5 -3.3756216670494013 2314 6 -0.7613453962556349 2314 7 -0.04694202375715406 2314 8 0.36496189888372044 2314 9 0.25657502855852243 2314 10-11 0.33769553967687216 2314 12-13 0.22218107658282094 2314 14-15 0.45198750277002375 2314 16-17 0.47036700526093256 2314 18-19 0.9292081545760809 2314 20-21 0.788520607107948 2314 22-23 0.6283186513576382 2314 24-25 0.8689033650659681 2314 26-27 0.6224652482742599 2314 28-29 0.5912879595112059 2314 30-31 0.38415182718740226 2314 32-33 0.544229988977996 2314 34-35 0.6675407481591762 2314 36-37 0.4707967299830145 2314 38-39 0.13315553355771215 2314 40-41 0.32381601839500007 2314 42-43 0.35738116792073527 2314 44-45 0.6136932161882171 2314 46-47 0.4726815520273817 2314 48-49 0.24972692459515855 2314 50-51 0.5998473177124026 2314 52-53 0.4047143712232426 2314 54-55 0.12801620167674344 2314 56-57 0.2792693516523954 2314 58-59 0.37375991809272335 2314 60-61 0.43231311521431337 2314 62-63 0.39607713745749606 2314 64-65 0.3832664817586746 2314 66-67 1.0031887958067536 2314 68-69 0.7898291850705199 2314 70-71 1.0092717850918156 2314 72-73 0.6033725094940223 2314 74-75 0.8414932559094517 2314 76-77 0.4343686440342367 2314 78-79 0.515113908872685 2314 80-81 0.7015397942773802 2314 82-83 0.6704413640715181 2314 84-85 0.47718754920952833 2314 86-87 0.6916353314308523 2314 88-89 0.8622495119829203 2314 90-91 0.6944433335815674 2314 92-93 0.7571542312206851 2314 94-95 0.7740653156924999 2314 96-97 0.7816191724256036 2314 98-99 0.8883709675262885 2314 100-101 1.2263120489664594 2314 102-103 1.0211060072722482 2314 104-105 1.0620074355814673 2314 106-107 1.1186842401857326 2314 108-109 1.2234332659619085 2314 110-111 1.2300440358835125 2314 112-113 1.254019909267619 2314 114-115 1.522859691732105 2314 116-117 1.7876297559290784 2314 118-119 1.248261160107326 2314 120-121 1.278147659366283 2315 1 -0.3440275086240199 2315 2 0.19834097801493655 2315 3 -0.319782633805449 2315 4 0.12446871573107643 2315 5 0.20137967163868353 2315 6 -0.13280456626902293 2315 7 0.3236068383579749 2315 8 -0.08052672226754964 2315 9 0.03847596564018119 2315 10-11 -0.1552897347541915 2315 12-13 -0.5102285619713953 2315 14-15 -0.8924167810318551 2315 16-17 -0.3549208126774914 2315 18-19 -0.2856512831749214 2315 20-21 -0.16830670212899435 2315 22-23 0.006390940514258148 2315 24-25 0.04377618969783015 2315 26-27 0.1786633741109398 2315 28-29 0.05434016834654898 2315 30-31 0.282759591578305 2315 32-33 0.3689957185629993 2315 34-35 0.26036538001994813 2315 36-37 6.904012465724918E-6 2315 38-39 0.14046477050550266 2315 40-41 0.13550276538295236 2315 42-43 0.18728745975473515 2315 44-45 0.16720057897268958 2315 46-47 0.05328396166593308 2315 48-49 0.20078448818284755 2315 50-51 0.28268533645403693 2315 52-53 0.4704707299916464 2315 54-55 0.24400013741971804 2315 56-57 0.14546747748907762 2315 58-59 -0.029653736525752095 2315 60-61 0.08713239232274361 2315 62-63 0.028941929960843993 2315 64-65 0.09001346971047752 2315 66-67 0.15802145979604987 2315 68-69 0.06130174196475835 2315 70-71 -0.1470869833151447 2315 72-73 0.1569200329210645 2315 74-75 0.2224169506885687 2315 76-77 0.23585458780933521 2315 78-79 0.3012049396625116 2315 80-81 0.07226268584364703 2315 82-83 0.16208795041689683 2315 84-85 0.22116345282398697 2315 86-87 0.063067727682526 2315 88-89 0.03582380917167427 2315 90-91 0.08342593063645509 2315 92-93 0.43826534233179615 2315 94-95 0.1252433612078967 2315 96-97 0.06902211753939369 2315 98-99 -2.0367829816035616 2315 100-101 -11.050957027338761 2315 102-103 -4.690018490719719 2315 104-105 -1.6290367411253612 2315 106-107 -0.2913559204568408 2315 108-109 0.2762846715843992 2315 110-111 0.25609490603076424 2315 112-113 0.804970377808452 2315 114-115 0.9544661174349152 2315 116-117 0.848419581899627 2315 118-119 0.7204566085678294 2315 120-121 0.38069116672906844 2316 1 -1.119299376236313 2316 2 -2.8027937737581112 2316 3 -1.0596407898338178 2316 4 -3.8427180454982377 2316 5 -4.303537586044531 2316 6 -1.4415043298624042 2316 7 -8.158426258568742 2316 8 -3.3818978806599844 2316 9 -1.8457320721848802 2316 10-11 -0.7704433990567878 2316 12-13 -0.4174626045245873 2316 14-15 -4.463149641551951 2316 16-17 -3.1404763682330454 2316 18-19 -5.337069722891243 2316 20-21 -1.9073137943275782 2316 22-23 -4.479140974379355 2316 24-25 -1.8992970963541822 2316 26-27 -3.696324805558085 2316 28-29 -2.4484967110860794 2316 30-31 -0.8864839072396649 2316 32-33 -0.47048418687435145 2316 34-35 -0.7818568422022736 2316 36-37 -0.2630663820395469 2316 38-39 -0.7303153713384631 2316 40-41 -0.7672631920638509 2316 42-43 -0.5416959917819071 2316 44-45 -0.8121847638169086 2316 46-47 -0.48841816599363597 2316 48-49 -0.3208703581528596 2316 50-51 -0.05956052643012555 2316 52-53 -0.2230990099610679 2316 54-55 -0.5103970257008541 2316 56-57 -0.5629249574990993 2316 58-59 -0.9342636656037584 2316 60-61 -0.44903782044320906 2316 62-63 -0.7503724908429419 2316 64-65 -0.7895609983746255 2316 66-67 -0.8722149468233376 2316 68-69 -0.6857668159548638 2316 70-71 -0.7603257540007284 2316 72-73 -0.6770043169607334 2316 74-75 -0.7587887230702961 2316 76-77 -0.3491099511977538 2316 78-79 -0.44593454024292356 2316 80-81 -0.6549713567095452 2316 82-83 -0.8108198510015399 2316 84-85 -0.5852195258994151 2316 86-87 -0.5932916103789374 2316 88-89 -0.6700627156510208 2316 90-91 -0.7415858896945124 2316 92-93 -0.6705053432474024 2316 94-95 -0.2919434000214203 2316 96-97 -0.5146894663849544 2316 98-99 0.2125314392002231 2316 100-101 0.2448585754981174 2316 102-103 0.005584346159714926 2316 104-105 -0.027405535451602958 2316 106-107 0.052970320677911076 2316 108-109 -0.010878448982978739 2316 110-111 -0.4775693965697023 2316 112-113 -0.24075066238067322 2316 114-115 -0.32943459178494194 2316 116-117 0.40447159135589317 2316 118-119 0.08423911447799526 2316 120-121 -0.21316226116691084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6826.0 3 1150.0 4 1012.0 5 1109.0 6 1216.0 7 1201.0 8 1566.0 9 1752.0 10 2077.0 11 2530.0 12 2641.0 13 2977.0 14 3802.0 15 3901.0 16 3096.0 17 2910.0 18 3070.0 19 3283.0 20 3528.0 21 3690.0 22 4195.0 23 4629.0 24 5134.0 25 5783.0 26 6609.0 27 7425.0 28 8560.0 29 10221.0 30 11888.0 31 14250.0 32 17485.0 33 22083.0 34 28434.0 35 38345.0 36 52922.0 37 59725.0 38 21465.0 39 100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.49679745949729 18.29619462834383 13.940201302545885 22.266806609613006 2 13.79261656764888 20.615340335176114 38.99816446003628 26.59387863713873 3 21.47576524728592 24.397014172100253 31.35227830824793 22.774942272365898 4 11.08627177549099 15.52940793453991 42.44270749964053 30.941612790328577 5 10.690477547066916 37.25279525518828 37.1800819923434 14.8766452054014 6 26.829552854352073 35.834056869980465 19.780497069676578 17.555893205990884 7 23.670398168517046 32.734231533948346 24.873665108784987 18.721705188749617 8 22.36044726319844 30.42138443259314 22.432603989778702 24.785564314429717 9 21.525453154585257 18.95246827511 26.712927044960093 32.809151525344646 10-11 23.42238243646809 23.792222375926634 29.361792157277588 23.42360303032769 12-13 26.316924416452043 22.74558787430002 28.711468068456607 22.22601964079133 14-15 22.380444777163873 24.13739323368025 30.598840725943166 22.883321263212714 16-17 19.383755249080394 28.1966492691978 30.627366833407482 21.792228648314328 18-19 18.437999834490768 30.027444686678813 30.857704115617697 20.676851363212727 20-21 20.00694539589435 26.82292903080527 28.903427797047936 24.266697776252443 22-23 18.68566786068383 26.54515812702516 30.57045628093266 24.198717731358343 24-25 21.857584047547032 25.862651246251477 29.57299085435737 22.706773851844115 26-27 20.710861774767174 24.079208296823584 33.5230031766873 21.68692675172195 28-29 18.922314094433176 28.34175558072809 31.51580104051497 21.220129284323765 30-31 19.40354999403089 26.485088071542528 30.572844879044077 23.538517055382513 32-33 20.07702575845547 26.030039774484642 31.537964826983707 22.354969640076185 34-35 22.120870921793394 25.142101336227363 30.424065658278504 22.31296208370074 36-37 21.10906858588668 24.772047401659194 32.88269649507194 21.23618751738219 38-39 20.953066985730313 26.8493685876957 31.320464975575078 20.877099450998905 40-41 19.664758845322673 28.01567135731156 31.337830889884028 20.98173890748174 42-43 19.12175654117877 27.942309570577446 28.69721748944621 24.238716398797575 44-45 19.712402555035126 26.521579303618637 28.818208084252678 24.94781005709356 46-47 21.67718428163695 27.60046447524065 29.61911402433069 21.103237218791712 48-49 21.268901190875066 26.63075547790322 29.807824045122754 22.29251928609895 50-51 18.22376150049787 27.622764677633345 32.445851681222884 21.7076221406459 52-53 19.593150075422965 28.86759742585217 28.75961778462674 22.779634714098126 54-55 18.69436282290251 27.05163998252711 30.12689549639011 24.12710169818027 56-57 21.25682704282214 25.443266019301575 29.00730117128043 24.292605766595855 58-59 22.807669511697267 25.696146391811304 28.85059045800336 22.64559363848807 60-61 21.524110607581886 26.075276111910124 31.77688529483596 20.62372798567203 62-63 19.823802889317882 27.46669850189044 30.73980266683308 21.9696959419586 64-65 18.759258756816887 29.938139295113547 29.626122581870472 21.676479366199093 66-67 19.709956181406266 27.24322711055038 27.751821252696267 25.29499545534709 68-69 19.031914085393648 27.558797673055636 29.352031735006186 24.05725650654453 70-71 21.73344155579746 26.11007201861137 28.30198410867128 23.854502316919895 72-73 20.542950801870465 24.761542794703516 32.076854686004765 22.61865171742126 74-75 18.924678928099713 28.291928711925102 30.504579372394534 22.278812987580647 76-77 19.276946510169342 26.700252231118643 29.607697800512067 24.415103458199955 78-79 20.003040615290224 26.45525340949351 30.29525188918586 23.246454086030408 80-81 21.559584479867137 26.14558401311255 28.98491731388161 23.30991419313871 82-83 19.933568797152716 25.526875181552377 31.924577373211964 22.614978648082943 84-85 20.102610864376445 26.978298657048764 30.148352893444546 22.770737585130245 86-87 19.874878908507746 28.02213159015855 29.713342179456916 22.38964732187679 88-89 19.021613033219403 26.347168080715434 28.49965197168556 26.131566914379604 90-91 19.255376457162708 24.275709906738875 29.871920299933645 26.596993336164775 92-93 20.74890806289883 24.847454453434427 30.678310377439132 23.725327106227603 94-95 19.310656414973767 24.4544121218535 31.497634485631217 24.73729697754152 96-97 16.39145631278731 26.10093187085149 33.80148491476394 23.706126901597262 98-99 18.003984539552256 28.27123097300936 29.68251253989968 24.042271947538705 100-101 16.31047355657995 26.655521072365396 30.254453375093128 26.77955199596153 102-103 18.54530702843366 26.02350725664101 29.676452990235386 25.754732724689944 104-105 20.600827511361324 25.576341314522143 29.403920504646276 24.418910669470257 106-107 19.773040216513934 25.54172573544873 31.491110854863763 23.19412319317357 108-109 19.510864994071667 26.11709069400845 29.925604160957647 24.446440150962236 110-111 19.45638312304979 27.117080450413784 28.37905304571971 25.047483380816715 112-113 20.597979151178375 24.290206697453318 27.25256269026856 27.859251461099742 114-115 19.78322099573443 24.48037076834576 28.53700628439321 27.199401951526596 116-117 22.14537332551727 24.081884105169458 26.943838337784147 26.828904231529126 118-119 20.579339243333607 23.695998197961035 30.060533854042987 25.66412870466237 120-121 19.17340413840637 27.180491881793156 29.324724203476848 24.321379776323624 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1046.0 1 1260.5 2 885.0 3 299.5 4 286.5 5 259.0 6 253.0 7 294.5 8 321.0 9 333.0 10 348.5 11 338.0 12 345.5 13 392.5 14 430.0 15 466.5 16 494.0 17 503.5 18 570.5 19 629.5 20 687.0 21 802.0 22 921.0 23 1045.5 24 1252.5 25 1478.5 26 1780.5 27 2355.0 28 3160.5 29 4063.5 30 5045.0 31 5959.5 32 6856.0 33 7585.5 34 8137.0 35 8835.0 36 9426.5 37 10054.0 38 10678.5 39 10952.0 40 11183.0 41 11515.5 42 11721.0 43 12097.5 44 12359.0 45 12713.0 46 14315.0 47 20099.0 48 32705.5 49 32713.0 50 20271.5 51 13586.5 52 10177.5 53 8371.0 54 7359.0 55 6524.0 56 5554.5 57 4435.0 58 3455.0 59 2742.0 60 2170.5 61 1752.0 62 1352.0 63 976.0 64 680.0 65 451.0 66 302.0 67 214.0 68 147.5 69 97.0 70 65.0 71 48.0 72 35.5 73 20.5 74 12.5 75 10.0 76 7.5 77 5.0 78 4.5 79 4.5 80 3.0 81 2.5 82 3.0 83 3.0 84 1.5 85 0.0 86 1.0 87 2.0 88 3.0 89 2.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2710754448589603 2 1.155693926299686 3 1.0867173032019108 4 1.0732977267237445 5 1.0786655573150112 6 1.0816178641402077 7 1.053973536595185 8 1.059609758716015 9 1.0606833248342682 10-11 1.0512896212995517 12-13 1.0516922085938967 14-15 1.049679272122172 16-17 1.061220107893395 18-19 1.0817520599049895 20-21 1.0737003140180896 22-23 1.05920717142167 24-25 1.0586703883625432 26-27 1.064709197777718 28-29 1.0796049276684827 30-31 1.0805442980219544 32-33 1.0767868166080679 34-35 1.0775919911967577 36-37 1.0836308006119326 38-39 1.076652620843286 40-41 1.0787997530797928 42-43 1.0741029013124346 44-45 1.0694060495450763 46-47 1.0741029013124346 48-49 1.072358356370273 50-51 1.0786655573150112 52-53 1.0741029013124346 54-55 1.0786655573150112 56-57 1.0786655573150112 58-59 1.0562548645964733 60-61 1.095037440618374 62-63 1.0804101022571728 64-65 1.0789339488445744 66-67 1.080275906492391 68-69 1.0837649963767144 70-71 1.1307335140502965 72-73 1.1359671488767815 74-75 1.1416033709976114 76-77 1.1479105719423495 78-79 1.1389194557019782 80-81 1.0971845728548808 82-83 1.137711693818943 84-85 1.1032233822700557 86-87 1.0915483507340509 88-89 1.0965135940309723 90-91 1.102686599210929 92-93 1.098660726267479 94-95 1.091145763439706 96-97 1.0961110067366273 98-99 1.1181191121608203 100-101 1.109396387450012 102-103 1.091145763439706 104-105 1.0775919911967577 106-107 1.0796049276684827 108-109 1.0792023403741378 110-111 1.0843017794358412 112-113 1.0821546471993344 114-115 1.0891328269679807 116-117 1.1052363187417804 118-119 1.1053705145065622 120-121 1.091414154969269 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 372590.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.55680611567468 #Duplication Level Percentage of deduplicated Percentage of total 1 90.69801479040073 63.08664229852498 2 6.023531152363612 8.379551769933643 3 1.4150706039237002 2.9528337491133443 4 0.5961547653165236 1.6586648570422784 5 0.3738751847446244 1.3002781868371944 6 0.2057250001963102 0.858574437108113 7 0.13522722436107532 0.6584181678510908 8 0.10016713754180824 0.557384493212614 9 0.07296137765000449 0.4567464359220493 >10 0.3309903378771615 4.040328232285923 >50 0.020636843210619964 0.9565941528288404 >100 0.01752184800877013 2.7405934972726294 >500 0.0050618672025335935 2.572133258582682 >1k 0.003893744001948918 4.65161511173608 >5k 0.0011681232005846752 5.1296413517485515 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8085 2.1699455165194985 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5552 1.4901097721355914 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5303 1.423280281274323 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4517 1.2123245390375479 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2769 0.7431761453608524 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1845 0.4951823720443383 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1503 0.4033924689336804 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1163 0.3121393488821493 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1151 0.30891865052738937 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1147 0.30784508440913605 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1067 0.2863737620440699 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1010 0.2710754448589603 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1003 0.26919670415201696 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 952 0.25550873614428726 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 942 0.252824820848654 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 929 0.2493357309643308 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 856 0.2297431493062079 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 810 0.21739713894629487 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 808 0.21686035588716818 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 786 0.210955742236775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 659 0.17687001798223248 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 624 0.16747631444751604 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 600 0.1610349177379962 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 520 0.13956359537293003 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 510 0.13687968007729676 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 501 0.13446415631122682 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 454 0.12184975442175044 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 439 0.11782388147830054 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 439 0.11782388147830054 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 424 0.11379800853485066 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 407 0.10923535253227407 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 405 0.10869856947314743 No Hit ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 387 0.10386752194100754 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.68391529563327E-4 0.0 10-11 0.0 0.0 0.0 4.025872943449905E-4 0.0 12-13 0.0 0.0 0.0 5.36783059126654E-4 0.0 14-15 0.0 0.0 0.0 9.393703534716445E-4 0.0 16-17 0.0 0.0 0.0 0.002147132236506616 0.0 18-19 0.0 1.341957647816635E-4 0.0 0.003220698354759924 0.0 20-21 0.0 2.68391529563327E-4 2.68391529563327E-4 0.005099439061703213 0.0 22-23 0.0 2.68391529563327E-4 2.68391529563327E-4 0.009125312005153118 0.0 24-25 0.0 2.68391529563327E-4 2.68391529563327E-4 0.012211814595131378 0.0 26-27 0.0 2.68391529563327E-4 2.68391529563327E-4 0.016640274832926273 0.0 28-29 0.0 2.68391529563327E-4 2.68391529563327E-4 0.02777852330980434 0.0 30-31 0.0 2.68391529563327E-4 2.68391529563327E-4 0.048444671086180516 0.0 32-33 0.0 2.68391529563327E-4 2.68391529563327E-4 0.08105424192812474 0.0 34-35 0.0 2.68391529563327E-4 2.68391529563327E-4 0.11044311441530905 0.0 36-37 0.0 2.68391529563327E-4 2.68391529563327E-4 0.13553772242948012 0.0 38-39 0.0 2.68391529563327E-4 2.68391529563327E-4 0.17633323492310582 0.0 40-41 0.0 2.68391529563327E-4 2.68391529563327E-4 0.23068251965967954 0.0 42-43 0.0 2.68391529563327E-4 2.68391529563327E-4 0.2777852330980434 0.0 44-45 0.0 2.68391529563327E-4 2.68391529563327E-4 0.29120480957620976 0.0 46-47 0.0 2.68391529563327E-4 2.68391529563327E-4 0.3153600472369092 0.0 48-49 0.0 2.68391529563327E-4 2.68391529563327E-4 0.34340696207627686 0.0 50-51 0.0 8.05174588689981E-4 2.68391529563327E-4 0.3714538769156445 0.0 52-53 0.0 8.05174588689981E-4 2.68391529563327E-4 0.4448589602512145 0.0 54-55 0.0 8.05174588689981E-4 2.68391529563327E-4 0.47505300732708877 0.0 56-57 0.0 8.05174588689981E-4 2.68391529563327E-4 0.5752972436189914 0.0 58-59 0.0 8.05174588689981E-4 2.68391529563327E-4 0.7364663571217692 0.0 60-61 0.0 8.05174588689981E-4 2.68391529563327E-4 0.8516063233044365 0.0 62-63 0.0 8.05174588689981E-4 2.68391529563327E-4 0.9103840682788051 0.0 64-65 0.0 8.05174588689981E-4 2.68391529563327E-4 1.0828256260232427 0.0 66-67 0.0 8.05174588689981E-4 2.68391529563327E-4 1.654096996698784 0.0 68-69 0.0 8.05174588689981E-4 2.68391529563327E-4 2.286830027644328 0.0 70-71 0.0 8.05174588689981E-4 2.68391529563327E-4 2.693040607638423 0.0 72-73 0.0 8.05174588689981E-4 2.68391529563327E-4 3.1258219490592873 0.0 74-75 0.0 8.05174588689981E-4 2.68391529563327E-4 3.621272712633189 0.0 76-77 0.0 8.05174588689981E-4 2.68391529563327E-4 3.765667355538259 0.0 78-79 0.0 8.05174588689981E-4 2.68391529563327E-4 4.040500281811106 0.0 80-81 0.0 8.05174588689981E-4 2.68391529563327E-4 4.471268686760245 0.0 82-83 0.0 8.05174588689981E-4 2.68391529563327E-4 4.827155854961218 0.0 84-85 0.0 8.05174588689981E-4 2.68391529563327E-4 5.529670683593226 0.0 86-87 0.0 8.05174588689981E-4 2.68391529563327E-4 7.3559408465068845 0.0 88-89 0.0 8.05174588689981E-4 2.68391529563327E-4 9.878687028637376 0.0 90-91 0.0 8.05174588689981E-4 2.68391529563327E-4 12.02742961432137 0.0 92-93 0.0 8.05174588689981E-4 2.68391529563327E-4 13.67132773289675 0.0 94-95 0.0 8.05174588689981E-4 2.68391529563327E-4 15.02026356048203 0.0 96-97 0.0 8.05174588689981E-4 2.68391529563327E-4 16.15019189994364 0.0 98-99 0.0 8.05174588689981E-4 2.68391529563327E-4 16.95630585898709 0.0 100-101 0.0 8.05174588689981E-4 2.68391529563327E-4 17.43847124184761 0.0 102-103 0.0 8.05174588689981E-4 2.68391529563327E-4 17.825626023242705 0.0 104-105 0.0 8.05174588689981E-4 2.68391529563327E-4 18.31718510963794 0.0 106-107 0.0 8.05174588689981E-4 2.68391529563327E-4 18.98843232507582 0.0 108-109 0.0 8.05174588689981E-4 2.68391529563327E-4 19.74274671891355 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 90 0.0 70.39114 2 TACCTGG 495 0.0 69.8094 2 ACCTGGG 540 0.0 61.682457 3 TAACGCA 105 0.0 60.16318 4 GTATAAC 120 0.0 57.67917 1 ATAACGC 105 1.8189894E-12 54.6938 3 TACGGCG 35 0.008963457 49.224426 4 GTACCTG 780 0.0 45.84754 1 CCTGGGG 390 0.0 35.34061 4 TAAGGTG 120 1.2197524E-6 33.509056 5 CCACGTG 70 0.0033171952 32.811825 9 GTCCTTA 95 3.937812E-4 30.35746 1 AAGACGG 95 4.0332024E-4 30.233736 5 CCTGGGA 370 0.0 29.490307 4 ACGCTAC 60 4.6912523E-5 28.72595 40-41 GATAGGT 100 5.4590456E-4 28.710348 9 TACTGAG 105 7.2556996E-4 27.3469 7 TCTCGGG 160 0.0 26.92692 114-115 GAGTAGG 215 5.5933924E-9 26.827522 1 ACTGATC 110 9.528323E-4 26.100317 8 >>END_MODULE