##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003789_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2595869 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.608748361338726 31.0 31.0 34.0 30.0 34.0 2 31.751341073066477 31.0 31.0 34.0 30.0 34.0 3 31.839909872185384 31.0 31.0 34.0 30.0 34.0 4 35.55269352960415 37.0 35.0 37.0 33.0 37.0 5 35.42979017816384 37.0 35.0 37.0 33.0 37.0 6 35.50012577676301 37.0 35.0 37.0 33.0 37.0 7 35.45697529420784 37.0 35.0 37.0 33.0 37.0 8 35.4875927868471 37.0 35.0 37.0 33.0 37.0 9 37.00866453584522 39.0 37.0 39.0 33.0 39.0 10-11 36.900193345658046 39.0 37.0 39.0 32.5 39.0 12-13 36.880239911952415 39.0 37.0 39.0 33.0 39.0 14-15 37.819059243744576 39.0 37.0 41.0 32.5 41.0 16-17 37.914371642020456 39.5 37.0 41.0 33.0 41.0 18-19 38.038187404680286 40.0 37.0 41.0 33.0 41.0 20-21 37.90930975330419 40.0 37.0 41.0 32.5 41.0 22-23 37.85250141667395 40.0 37.0 41.0 32.5 41.0 24-25 37.58289825102885 39.0 36.5 41.0 32.0 41.0 26-27 37.378953059649774 39.0 36.0 41.0 31.5 41.0 28-29 37.31361655769224 39.0 36.0 41.0 31.5 41.0 30-31 37.14919319888639 39.0 36.0 40.0 31.0 41.0 32-33 36.89299671901779 39.0 35.0 40.0 30.0 41.0 34-35 36.80154410719493 39.0 35.0 40.0 30.0 41.0 36-37 36.62408522926234 38.5 35.0 40.0 30.0 41.0 38-39 36.36937283815169 38.0 35.0 40.0 30.0 41.0 40-41 36.604318053029644 39.0 35.0 40.0 30.0 41.0 42-43 36.47847233431271 39.0 35.0 40.5 30.0 41.0 44-45 36.109000300092184 39.0 35.0 40.0 29.5 41.0 46-47 35.98112443270442 39.0 35.0 40.0 29.0 41.0 48-49 35.66740116700804 38.5 35.0 40.0 28.0 41.0 50-51 35.45274260758151 38.0 34.0 40.0 27.5 41.0 52-53 35.29324900447596 38.0 34.0 40.0 27.0 41.0 54-55 35.14425496818214 38.0 34.0 40.0 26.5 41.0 56-57 34.770305435289686 38.0 33.5 40.0 25.5 41.0 58-59 34.46165157024488 38.0 33.0 40.0 25.0 41.0 60-61 33.912098800055006 37.0 32.5 40.0 23.0 41.0 62-63 33.74349591601117 37.0 32.5 40.0 23.0 41.0 64-65 33.296284596795914 36.0 32.0 39.0 22.0 40.5 66-67 32.75203871998163 36.0 31.5 39.0 22.0 40.0 68-69 32.38097011058724 35.5 31.0 38.0 21.0 39.5 70-71 31.71983004535283 35.0 31.0 37.5 20.5 39.0 72-73 31.32773649209571 34.5 30.5 37.0 19.5 39.0 74-75 31.026310457114747 34.0 30.5 36.5 18.5 38.5 76-77 29.955380645171232 33.5 29.0 35.0 18.0 37.0 78-79 30.389313944578866 34.0 30.0 35.0 18.0 37.0 80-81 30.12833756248871 34.0 30.0 35.0 14.0 37.0 82-83 29.8578481810908 34.0 29.5 35.0 12.5 36.0 84-85 29.566079798325724 34.0 29.0 35.0 8.5 36.0 86-87 29.434524430932377 34.0 29.0 35.0 7.0 36.0 88-89 29.103462655472985 34.0 29.0 35.0 2.0 35.0 90-91 28.667937788848356 34.0 29.0 35.0 2.0 35.0 92-93 28.50102701638642 34.0 29.0 35.0 2.0 35.0 94-95 28.149260228462992 34.0 29.0 35.0 2.0 35.0 96-97 27.78988462052592 34.0 28.5 35.0 2.0 35.0 98-99 27.451338453519803 34.0 27.0 35.0 2.0 35.0 100-101 26.434043281845113 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.20584896801722863 1101 2 0.29129325266693584 1101 3 0.20779779063684245 1101 4 0.1987663267769335 1101 5 0.14591583876183023 1101 6 0.23935658990254893 1101 7 0.2033048199154308 1101 8 0.1601619971133843 1101 9 0.32383577393380847 1101 10-11 0.32372314629837007 1101 12-13 0.3587360371285939 1101 14-15 0.47556491258571043 1101 16-17 0.4407635655762121 1101 18-19 0.39166885092559767 1101 20-21 0.30242144887755984 1101 22-23 0.20622287644891202 1101 24-25 0.4529702814667971 1101 26-27 0.5058106361054939 1101 28-29 0.4570138776509154 1101 30-31 0.6143539921813144 1101 32-33 0.5594165866472665 1101 34-35 0.42921708743351417 1101 36-37 0.48708524171161116 1101 38-39 0.4223366574319769 1101 40-41 0.47661001432185657 1101 42-43 0.46125437654165324 1101 44-45 0.6490120800005599 1101 46-47 0.5573136956098423 1101 48-49 0.5833806073680847 1101 50-51 0.5468617352398724 1101 52-53 0.5120986781869519 1101 54-55 0.5252559408421575 1101 56-57 0.45791630070543476 1101 58-59 0.5154024267025576 1101 60-61 0.3575736285064295 1101 62-63 0.32583093133727203 1101 64-65 0.2861894586285274 1101 66-67 0.3871574656964114 1101 68-69 0.5341451091682998 1101 70-71 0.6366407193037595 1101 72-73 0.4590181141186491 1101 74-75 0.3516167274213231 1101 76-77 0.4059106548456626 1101 78-79 0.48690678448813074 1101 80-81 0.4835875159811778 1101 82-83 0.546200609560632 1101 84-85 0.5314539278727679 1101 86-87 0.4114329782398549 1101 88-89 0.5827275455040102 1101 90-91 0.7031463884681948 1101 92-93 0.6515291030220887 1101 94-95 0.6631086279096472 1101 96-97 0.707428524927554 1101 98-99 0.6666958854136062 1101 100-101 0.9044951507142578 1102 1 -1.1630173769106023E-4 1102 2 0.08270556978083476 1102 3 0.15387900907441576 1102 4 0.10353851894620902 1102 5 0.19204965908730998 1102 6 0.07124023234029409 1102 7 0.03668557028336039 1102 8 0.07221469826685478 1102 9 0.12647750839870753 1102 10-11 0.12572966237385685 1102 12-13 0.10188449720500614 1102 14-15 0.05562096808815653 1102 16-17 0.16705298024763238 1102 18-19 0.03472220365708267 1102 20-21 0.10699221466561681 1102 22-23 0.13914040227236768 1102 24-25 0.19295653495826315 1102 26-27 0.11655068307052119 1102 28-29 0.11776294271550825 1102 30-31 0.20131690748105058 1102 32-33 0.22274829214345715 1102 34-35 0.3094481945149141 1102 36-37 0.25452643831075505 1102 38-39 0.28482456128737255 1102 40-41 0.20398300424950122 1102 42-43 0.3836078006645778 1102 44-45 0.5272382663459609 1102 46-47 0.34197100561045346 1102 48-49 0.49080246638467884 1102 50-51 0.5742250940439817 1102 52-53 0.5826303354093554 1102 54-55 0.504947010737375 1102 56-57 0.5602938513836904 1102 58-59 0.562709190838568 1102 60-61 0.430882616663844 1102 62-63 0.29409981369412463 1102 64-65 0.19811711766430307 1102 66-67 0.28944931119251294 1102 68-69 0.26063892671079714 1102 70-71 0.27983122691567175 1102 72-73 0.5043851005474806 1102 74-75 0.4285479855725143 1102 76-77 0.36309580536668307 1102 78-79 0.5639584316835347 1102 80-81 0.6071344829970897 1102 82-83 0.5101771054831339 1102 84-85 0.48327183198370705 1102 86-87 0.5286491064129741 1102 88-89 0.501462207902847 1102 90-91 0.5821991600824994 1102 92-93 0.5355908713170514 1102 94-95 0.717023162619288 1102 96-97 0.7449971448362973 1102 98-99 0.6138119326232463 1102 100-101 0.7816021990274926 1103 1 -0.059791151466132675 1103 2 0.04963361594943194 1103 3 0.027695398658185155 1103 4 0.02528206446762482 1103 5 0.05335614405867517 1103 6 0.0921697419189016 1103 7 0.1189112571704598 1103 8 0.13209865623210248 1103 9 0.16271615704150832 1103 10-11 0.11516800164771013 1103 12-13 0.0707486196982785 1103 14-15 0.08991345871240952 1103 16-17 0.13259566261960742 1103 18-19 0.04090806159575777 1103 20-21 -0.010399897955942095 1103 22-23 0.06375927296099348 1103 24-25 0.18526042189450465 1103 26-27 0.03103115035355586 1103 28-29 0.0388856384074856 1103 30-31 0.12839310414117477 1103 32-33 0.17486493942748638 1103 34-35 0.16152506256371169 1103 36-37 0.006041451821538146 1103 38-39 0.14313048211842982 1103 40-41 0.10515593980323246 1103 42-43 0.3599357227066591 1103 44-45 0.566509245155892 1103 46-47 0.609438170359148 1103 48-49 0.4270691264924551 1103 50-51 0.3940125562930348 1103 52-53 0.5179832985538937 1103 54-55 0.6579994651822432 1103 56-57 0.49832246131886393 1103 58-59 0.4669726870326798 1103 60-61 0.5802719832405785 1103 62-63 0.43708426276823076 1103 64-65 0.49994165221379205 1103 66-67 0.5820196329628118 1103 68-69 0.36540554288498583 1103 70-71 0.44891505211543503 1103 72-73 0.6196418930235446 1103 74-75 0.4438450874418578 1103 76-77 0.45168456203570173 1103 78-79 0.6049411691293685 1103 80-81 0.6951628365443661 1103 82-83 0.6187792847107971 1103 84-85 0.456255630093267 1103 86-87 0.6916551911497884 1103 88-89 0.6726310751454498 1103 90-91 0.6898894674811693 1103 92-93 0.6718062451527764 1103 94-95 0.7922887870068358 1103 96-97 0.8685177105792405 1103 98-99 0.6599793115123376 1103 100-101 0.7074491677793695 1104 1 -0.0450088369938868 1104 2 0.00239423940400485 1104 3 0.025111658938818948 1104 4 0.04456985571563621 1104 5 6.433680825495003E-4 1104 6 0.018340889744699496 1104 7 -0.08371196154195815 1104 8 -0.018331741965511128 1104 9 -0.06024670159451517 1104 10-11 -0.06033975680644943 1104 12-13 0.05462023314467501 1104 14-15 -0.03233865746532416 1104 16-17 0.08035286030084166 1104 18-19 0.010316062398359804 1104 20-21 -8.429270824450441E-5 1104 22-23 -0.029222618553042423 1104 24-25 0.05889285582868098 1104 26-27 0.05640617064170783 1104 28-29 0.06796461785742736 1104 30-31 -0.01922800315655593 1104 32-33 0.18546699045402448 1104 34-35 0.09114610040542459 1104 36-37 0.21674068098032961 1104 38-39 0.18315149738693748 1104 40-41 0.31014132630546953 1104 42-43 0.0662604286219235 1104 44-45 0.18871602198912996 1104 46-47 0.30640096484482626 1104 48-49 0.2948160673528264 1104 50-51 0.28072135157118083 1104 52-53 0.21092970238419895 1104 54-55 0.26565315651632915 1104 56-57 0.31942787794764627 1104 58-59 0.21269993614753702 1104 60-61 0.2499822744759257 1104 62-63 -0.046858856612431055 1104 64-65 0.1217225889005249 1104 66-67 0.21094826907573605 1104 68-69 0.06474346787200602 1104 70-71 -0.12517938491580693 1104 72-73 -0.14319010613526117 1104 74-75 -0.05930394708332898 1104 76-77 -0.14411482004691223 1104 78-79 0.07795400956936405 1104 80-81 0.08470449676987357 1104 82-83 0.14606874676188752 1104 84-85 0.1320085345244415 1104 86-87 0.2260570217017026 1104 88-89 0.06519280598939403 1104 90-91 0.016360602597476515 1104 92-93 0.03807790885831963 1104 94-95 0.11055072735231519 1104 96-97 0.011280152430927615 1104 98-99 0.1932231800292037 1104 100-101 0.3669267548290982 1105 1 -0.12416851555424557 1105 2 -0.005853472314839792 1105 3 -0.04973412099165131 1105 4 0.001607544159661245 1105 5 -0.14168891194655941 1105 6 -0.028646282006995705 1105 7 -0.08108821515584452 1105 8 0.005309072862736741 1105 9 -0.012385911175563535 1105 10-11 -0.04630513433760797 1105 12-13 0.02370504352462177 1105 14-15 -0.07329865526863699 1105 16-17 0.06684121674881993 1105 18-19 0.028840896941922267 1105 20-21 -0.06956208696924904 1105 22-23 0.09362648387422468 1105 24-25 0.15501240646063508 1105 26-27 0.014890857746763686 1105 28-29 0.00314666510220718 1105 30-31 0.082683516397978 1105 32-33 0.1187209706939143 1105 34-35 0.19875456749731768 1105 36-37 0.0340058860278063 1105 38-39 0.043472048236388616 1105 40-41 0.2505992282188316 1105 42-43 0.28123113818959666 1105 44-45 0.32214465370285694 1105 46-47 0.34760538077402003 1105 48-49 0.38863198166448853 1105 50-51 0.3271085879229645 1105 52-53 0.4741938567769708 1105 54-55 0.4228962067678239 1105 56-57 0.23411836475340664 1105 58-59 0.5224040764063247 1105 60-61 0.4361250132162553 1105 62-63 0.3010579711329413 1105 64-65 0.13617740099303433 1105 66-67 0.23555736265844018 1105 68-69 0.2368024476096835 1105 70-71 0.3751708935365947 1105 72-73 0.39388358927169875 1105 74-75 0.45087436072670783 1105 76-77 0.43294716207750383 1105 78-79 0.47572743812159146 1105 80-81 0.41611271369899683 1105 82-83 0.4088942853308133 1105 84-85 0.18399534859107547 1105 86-87 0.29390391659793735 1105 88-89 0.3970194060519745 1105 90-91 0.25759894441288367 1105 92-93 0.28010460300290774 1105 94-95 0.2266318141946364 1105 96-97 0.3779413032808101 1105 98-99 0.4360360587147163 1105 100-101 0.6454711066929271 1106 1 -0.11854686490217858 1106 2 -0.15076607217834592 1106 3 -0.1573898189031553 1106 4 -0.11376923327009791 1106 5 -0.027627727074758468 1106 6 -0.13686133230094555 1106 7 -0.07562683435150319 1106 8 -0.1397308937671724 1106 9 -0.25942967545788775 1106 10-11 -0.15467169801409852 1106 12-13 -0.19365350772086742 1106 14-15 -0.14119230614146971 1106 16-17 -0.1860123295134528 1106 18-19 -0.2717011547880901 1106 20-21 -0.32776158790380094 1106 22-23 -0.18506181886808548 1106 24-25 -0.27920770029394504 1106 26-27 -0.188536422728653 1106 28-29 -0.3192260503890907 1106 30-31 0.01376323631846077 1106 32-33 -0.18462633406610252 1106 34-35 -0.11811457694827254 1106 36-37 -0.04584193191413988 1106 38-39 -0.26970054116181785 1106 40-41 -0.05821264151468597 1106 42-43 0.12376070585372645 1106 44-45 0.2805201330529954 1106 46-47 0.2631248620516331 1106 48-49 0.35906617497115434 1106 50-51 0.36451673791006556 1106 52-53 0.34255499675198564 1106 54-55 0.22159993150277302 1106 56-57 0.2305881825446363 1106 58-59 0.28333161426875364 1106 60-61 0.058482550834561664 1106 62-63 0.07008232047451202 1106 64-65 0.0075231688607289016 1106 66-67 0.003603110678149335 1106 68-69 -8.777365626571054E-4 1106 70-71 0.17184524112686717 1106 72-73 0.29616860213043594 1106 74-75 0.1822005353401721 1106 76-77 0.04031920358633556 1106 78-79 0.10616878519496709 1106 80-81 0.32825219941322104 1106 82-83 0.1641126078892725 1106 84-85 0.3500672832179603 1106 86-87 0.32204427689729087 1106 88-89 0.19855199973818927 1106 90-91 0.03960223259823792 1106 92-93 0.13053144087880852 1106 94-95 0.03766888747616903 1106 96-97 0.14849352312433695 1106 98-99 0.1540911469639603 1106 100-101 0.2970765647834277 1107 1 -0.2759126619546741 1107 2 -0.29054436585138177 1107 3 -0.30576777929483967 1107 4 -0.18506038864772023 1107 5 -0.2561191965295251 1107 6 -0.28966579203308385 1107 7 -0.23403388024885174 1107 8 -0.24279580191050343 1107 9 -0.38303864804014864 1107 10-11 -0.4111354085510044 1107 12-13 -0.4707920346772241 1107 14-15 -0.3869367377755495 1107 16-17 -0.38555057753224986 1107 18-19 -0.31615614716429263 1107 20-21 -0.4096620407006313 1107 22-23 -0.5095993007619981 1107 24-25 -0.44524790380395274 1107 26-27 -0.2602004255507211 1107 28-29 -0.21264837451916918 1107 30-31 -0.284664198715852 1107 32-33 -0.4181689500182486 1107 34-35 -0.22223980193361115 1107 36-37 -0.3869549513383035 1107 38-39 -0.30054115381656743 1107 40-41 -0.19988377440569138 1107 42-43 -0.3469498290103985 1107 44-45 -0.4293924674200369 1107 46-47 -0.6195231924338032 1107 48-49 -0.6009346149887236 1107 50-51 -0.5447531348587233 1107 52-53 -0.4195114702001135 1107 54-55 -0.4641407478218653 1107 56-57 -0.357832333530709 1107 58-59 -0.5486069745223929 1107 60-61 -0.5827214161260486 1107 62-63 -0.604854295459937 1107 64-65 -0.5716953360857104 1107 66-67 -0.3960372532754377 1107 68-69 -0.39366673078709624 1107 70-71 -0.4136151386395355 1107 72-73 -0.33951705240240315 1107 74-75 -0.40395701807235085 1107 76-77 -0.6579227611256115 1107 78-79 -0.7229186272710102 1107 80-81 -0.4689523300533054 1107 82-83 -0.25036555210348155 1107 84-85 -0.35388912189253574 1107 86-87 -0.40784766884881307 1107 88-89 -0.594829907047906 1107 90-91 -0.800361088747227 1107 92-93 -0.8087422659194097 1107 94-95 -0.7321150108260319 1107 96-97 -0.8009783274043514 1107 98-99 -0.6668143922211129 1107 100-101 -0.7420426231378165 1108 1 -0.12219129864314127 1108 2 -0.16026812150547443 1108 3 -0.06734364997884867 1108 4 -0.10379924535724427 1108 5 -0.06257374647210412 1108 6 -0.1139018570018564 1108 7 -0.07467723911075552 1108 8 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-1.2152783932233326 2316 74-75 -0.786289228888414 2316 76-77 -0.4892204705015146 2316 78-79 -0.6520165209186572 2316 80-81 -0.6166371112736613 2316 82-83 -0.7781473163762875 2316 84-85 -0.8667828520607195 2316 86-87 -0.8698404551265924 2316 88-89 -0.7458398138069775 2316 90-91 -0.7577615224459961 2316 92-93 -0.8767825272867782 2316 94-95 -1.033116593783479 2316 96-97 -1.1539455474124765 2316 98-99 -1.2776373794038953 2316 100-101 -1.4701481545910227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 18.0 5 36.0 6 150.0 7 317.0 8 904.0 9 1765.0 10 2855.0 11 4548.0 12 6667.0 13 8968.0 14 11384.0 15 15384.0 16 19688.0 17 24607.0 18 22634.0 19 17174.0 20 15932.0 21 16083.0 22 17193.0 23 18891.0 24 22378.0 25 26823.0 26 33481.0 27 41624.0 28 52122.0 29 64692.0 30 79621.0 31 96986.0 32 119135.0 33 149302.0 34 191412.0 35 252851.0 36 360986.0 37 478145.0 38 362329.0 39 58763.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.544462316663605 18.970236395110437 16.99004333659813 17.495257951627828 2 14.331976880590167 27.05951787948027 32.42565417736208 26.18285106256749 3 28.628245173548017 24.692665951137172 29.52306811853639 17.156020756778425 4 10.341777647485292 15.665389894482349 44.54573786273499 29.447094595297372 5 11.27714842312921 40.812729764098265 35.75307536705435 12.157046445718178 6 26.613053278112265 40.2995682755948 14.18103147732031 18.906346968972628 7 22.82122094759019 34.9473336289312 20.04234420149861 22.18910122198 8 24.657022369002444 24.532555379335395 22.79914741460374 28.01127483705842 9 19.355483654991833 24.231846830483356 26.423675462821894 29.988994051702917 10-11 26.9592571890184 22.57712157277582 26.687267346695847 23.77635389150993 12-13 31.73888271668667 23.76220593651141 23.873170746740588 20.62574060006133 14-15 23.136290608244124 25.127661750630676 33.2922160184558 18.443831622669407 16-17 19.334508020242932 28.727297101664224 29.544942368047078 22.39325251004577 18-19 17.491849550189166 34.17501037224914 28.684402024909577 19.648738052652117 20-21 22.507459259411938 24.720897811854257 25.144345265901 27.627297662832806 22-23 20.443585729320596 27.166616330260247 27.516768957435435 24.873028982983726 24-25 25.95246524381623 26.619005042242115 26.669662452149932 20.758867261791718 26-27 22.920662010301534 22.34679646958659 33.866060799852065 20.866480720259812 28-29 19.603936783840005 32.97402247405724 28.422210069038773 18.999830673063975 30-31 21.552726987631665 26.55746477576364 26.888329784727777 25.001478451876917 32-33 21.036658950770374 27.57747502767533 28.78950704880547 22.59635897274883 34-35 26.25505710631909 25.140475978130993 27.527551513958414 21.0769154015915 36-37 23.6677976576696 22.930078401334068 34.1943365169587 19.20778742403763 38-39 21.745281909905838 27.000864474121855 30.5455359720285 20.708317643943804 40-41 20.380689383105675 28.197099678238573 29.979365969722373 21.44284496893338 42-43 20.35914751443136 30.34014200387353 23.830822060570288 25.46988842112482 44-45 22.20753062504527 24.708832834795043 27.1956681877093 25.88796835245039 46-47 25.84991413841292 27.676690433102603 27.42633865582072 19.04705677266375 48-49 24.422135138024387 27.20429227288793 26.896278739631196 21.477293849456483 50-51 17.26738211519922 27.561558397531407 34.438262467459545 20.73279701980983 52-53 21.616770826872678 32.18149724886848 22.784812320939533 23.416919603319307 54-55 19.345377569368 25.959845483989337 29.111629246325137 25.583147700317525 56-57 25.35794057391852 23.842824958992747 26.808330708676586 23.990903758412145 58-59 27.515745562194006 25.510546097859034 25.01829983372193 21.955408506225023 60-61 21.60067649953866 25.854841536917572 33.6398448982265 18.904637065317264 62-63 21.197722073557205 27.310133629002493 28.790932213119113 22.701212084321188 64-65 18.792653003311223 32.1988823274486 28.014261236487915 20.99420343275226 66-67 22.23079923169179 24.101662098542004 25.670477633660038 27.997061036106167 68-69 20.033537042381987 27.570046099464324 27.63195263581577 24.764464222337914 70-71 24.68819186343555 26.74223398344575 25.51052923194056 23.059044921178142 72-73 21.457244783000036 22.938947699311733 32.21314132536579 23.390666192322442 74-75 20.025024515048898 32.38107143524416 27.24786770599045 20.34603634371649 76-77 21.763504642798807 25.568311395736576 26.569987919169378 26.098196042295235 78-79 20.56504496702713 26.848448088128478 28.495174888037482 24.091332056806902 80-81 24.5114337946299 26.69861013920181 26.278321565445882 22.51163450072241 82-83 21.77876491255915 23.514522560226638 32.83892057312458 21.86779195408963 84-85 20.258733851766685 26.39890039172389 28.424569603558936 24.917796152950487 86-87 20.27657599001414 27.161869575100074 28.290210234737078 24.271344200148707 88-89 21.315762811827895 27.316907710460836 24.32456951695833 27.04275996075294 90-91 21.350903652168554 22.276224981749753 28.873511810174296 27.4993595559074 92-93 22.691263501052156 26.411414317977165 29.17673798207725 21.72058419889343 94-95 20.420485264504638 27.5181364536265 27.619860856580726 24.441517425288136 96-97 14.826046126358678 27.71112513063035 33.524240658425114 23.93858808458586 98-99 19.58108143252196 31.052430319101287 23.0079840338587 26.358504214518053 100-101 17.848843246296425 25.10723168767891 28.75920632079868 28.284718745225984 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 134.0 1 91.0 2 68.5 3 98.5 4 96.5 5 98.0 6 160.5 7 282.0 8 462.5 9 609.5 10 601.5 11 633.5 12 734.0 13 839.0 14 1043.0 15 1257.5 16 1483.0 17 1768.5 18 2112.5 19 2462.0 20 2795.5 21 3167.5 22 3580.5 23 4144.5 24 4820.0 25 5605.0 26 6796.0 27 8483.0 28 11346.5 29 16050.5 30 21830.5 31 27072.5 32 32697.5 33 37527.5 34 41044.0 35 45693.5 36 49594.0 37 53673.5 38 56312.5 39 51801.5 40 47412.5 41 46084.5 42 45160.0 43 46263.0 44 49045.5 45 57806.5 46 91354.5 47 238542.5 48 482888.5 49 457014.0 50 218215.0 51 96637.5 52 53228.5 53 38433.0 54 31799.0 55 25478.5 56 19932.0 57 15307.5 58 10935.0 59 8292.0 60 6074.0 61 3998.5 62 2547.5 63 1755.0 64 1128.5 65 613.5 66 361.5 67 234.0 68 149.0 69 83.5 70 35.5 71 19.5 72 15.0 73 8.5 74 4.0 75 3.5 76 2.0 77 1.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05878570914017618 2 0.004930911382662222 3 0.040602973416609234 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.852274517704861E-5 14-15 0.001714262160378663 16-17 0.0 18-19 0.0 20-21 0.010381879825214602 22-23 3.852274517704861E-5 24-25 0.0 26-27 0.005354661579609757 28-29 0.01149903943534901 30-31 0.009572902176496579 32-33 0.005605059423260573 34-35 0.0054124456973753295 36-37 0.019453986314409547 38-39 0.047305931077415694 40-41 0.006048070992796632 42-43 0.011787960024176876 44-45 0.010824891394750659 46-47 0.004680513539011406 48-49 0.0015986939248475176 50-51 0.0023691488283884895 52-53 0.001078636864957361 54-55 8.282390213065451E-4 56-57 0.01122938021910967 58-59 0.008359435703419548 60-61 0.005932502757265486 62-63 0.0067414804059835074 64-65 0.08725401782601509 66-67 0.041488996555681355 68-69 0.03189683300659625 70-71 0.020147395727596423 72-73 1.7335235329671875E-4 74-75 5.585798050672049E-4 76-77 8.089776487180208E-4 78-79 8.667617664835938E-4 80-81 0.0012905119634311285 82-83 0.0010978982375458852 84-85 0.0013675574537852257 86-87 0.007666026290232674 88-89 2.3113647106229166E-4 90-91 5.393184324786805E-4 92-93 2.50397843650816E-4 94-95 0.00500795687301632 96-97 0.010844152767339185 98-99 0.014503813559158802 100-101 0.01685370101495877 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2595869.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.902477345538223 #Duplication Level Percentage of deduplicated Percentage of total 1 76.27502447915305 15.943369712058697 2 12.308033291819072 5.145367741007567 3 4.100860400645531 2.5715442486512403 4 1.910038065535679 1.5969810957590074 5 1.0587807654737191 1.1065570482103015 6 0.6748106716177531 0.8463128865610511 7 0.4530491570122018 0.6628894818603916 8 0.3351185215852874 0.5603845844405385 9 0.28096829619458324 0.5285640101419573 >10 1.9978405215343282 8.552795718384305 >50 0.31024927351763854 4.566851172846558 >100 0.24701867897769358 10.359529080559472 >500 0.02485134741152861 3.3342744347478184 >1k 0.016816701255921617 6.913317498786542 >5k 0.0020553745979459753 2.9226944418536807 >10k+ 0.0044844536682457645 34.3885668441309 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 146200 5.632025344884506 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 90915 3.5022953777713743 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 67203 2.5888440441331975 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 66987 2.5805231311749552 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 52088 2.006572750782108 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 43199 1.6641440689033231 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 40693 1.5676060694896392 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 37965 1.4625160206466505 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 36950 1.423415434291946 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 31896 1.2287214801671424 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 28110 1.0828743669268364 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 25919 0.9984710322439229 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 23639 0.9106391732402521 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 23263 0.8961546210536818 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 22223 0.8560909660695513 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 20181 0.777427520418018 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 18737 0.7218006763823598 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 18369 0.707624306157206 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 17068 0.6575062146818657 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 16240 0.6256093816752694 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 15251 0.5875103866951683 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 14608 0.5627402615463262 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 12117 0.46678010331029807 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 10656 0.41049837260663 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 8963 0.3452793650218867 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 8448 0.3254401512557067 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7983 0.3075270747483791 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7609 0.2931195680521629 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 7402 0.2851453598005138 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 6598 0.25417307267816674 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 6355 0.2448120456001439 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6117 0.23564363224800633 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 5326 0.2051721408129609 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5017 0.1932686125532529 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 5014 0.19315304431772173 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 4770 0.18375349449452186 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4523 0.17423837643579088 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 4440 0.17104098858609584 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 4152 0.15994643797510583 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3691 0.14218745244848643 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3645 0.14041540617034218 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3611 0.13910563283432253 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3496 0.13467551713896192 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3253 0.12531449006093914 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3106 0.11965164651991297 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2970 0.11441255317583438 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 2956 0.11387323474335569 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2928 0.11279459787839834 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2882 0.1110225516002541 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 2849 0.10975130100941148 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2765 0.1065153904145394 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 2657 0.10235493393541815 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 2624 0.10108368334457556 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2603 0.10027470569585753 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.852274517704861E-5 0.0 10-11 0.0 0.0 0.0 2.3113647106229166E-4 0.0 12-13 0.0 0.0 0.0 3.274433340049132E-4 0.0 14-15 0.0 0.0 0.0 5.393184324786805E-4 0.0 16-17 0.0 0.0 0.0 7.319321583639236E-4 0.0 18-19 0.0 0.0 0.0 0.0010786368649573612 0.0 20-21 0.0 0.0 0.0 0.0019839213766180037 0.0 22-23 0.0 0.0 0.0 0.0029855127512212675 0.0 24-25 0.0 0.0 0.0 0.004622729421245833 0.0 26-27 0.0 0.0 0.0 0.006452559817155642 0.0 28-29 0.0 0.0 0.0 0.011036766493224427 0.0 30-31 0.0 0.0 0.0 0.019453986314409547 0.0 32-33 0.0 0.0 0.0 0.03191609437918477 0.0 34-35 0.0 0.0 0.0 0.04270246302875838 0.0 36-37 0.0 1.9261372588524304E-5 0.0 0.052949513245853314 0.0 38-39 0.0 3.852274517704861E-5 0.0 0.07340509093486614 0.0 40-41 0.0 3.852274517704861E-5 0.0 0.11138851767943606 0.0 42-43 0.0 3.852274517704861E-5 0.0 0.1762415591849974 0.0 44-45 0.0 3.852274517704861E-5 0.0 0.2323306761627802 0.0 46-47 0.0 5.7784117765572916E-5 0.0 0.38222267764667633 0.0 48-49 0.0 7.704549035409722E-5 0.0 0.482208462753706 0.0 50-51 0.0 7.704549035409722E-5 0.0 0.5499314487749574 0.0 52-53 0.0 7.704549035409722E-5 0.0 0.637936660131925 0.0 54-55 0.0 7.704549035409722E-5 0.0 0.7224940857955466 0.0 56-57 0.0 1.1556823553114583E-4 0.0 1.0091803554031424 0.0 58-59 0.0 1.1556823553114583E-4 0.0 1.39151860128535 0.0 60-61 0.0 1.1556823553114583E-4 0.0 1.7354304088534513 0.0 62-63 0.0 1.1556823553114583E-4 0.0 2.020537245908788 0.0 64-65 0.0 1.1556823553114583E-4 0.0 2.487606269807914 0.0 66-67 0.0 1.1556823553114583E-4 0.0 3.351787012364645 0.0 68-69 0.0 1.1556823553114583E-4 0.0 4.151153236160993 0.0 70-71 0.0 1.1556823553114583E-4 0.0 4.457177923847467 0.0 72-73 0.0 1.1556823553114583E-4 0.0 4.631994141460913 0.0 74-75 0.0 1.1556823553114583E-4 0.0 4.926962801281574 0.0 76-77 0.0 1.1556823553114583E-4 0.0 5.223645723262615 0.0 78-79 0.0 1.1556823553114583E-4 0.0 5.611300107979255 0.0 80-81 0.0 1.1556823553114583E-4 0.0 6.2916310491785215 0.0 82-83 0.0 1.1556823553114583E-4 0.0 7.10677233712487 0.0 84-85 0.0 1.1556823553114583E-4 0.0 8.91870891789994 0.0 86-87 0.0 1.1556823553114583E-4 0.0 10.94575650774365 0.0 88-89 0.0 1.1556823553114583E-4 0.0 12.700448289185626 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCTC 940 0.0 31.82651 5 ACGTTAA 370 0.0 29.519053 8 TATTCGC 210 1.8189894E-12 29.397953 9 CGTTAAC 375 0.0 29.126587 9 GACGTTA 410 0.0 27.797369 7 CCCGGCG 140 5.824568E-7 27.135527 8 TCAAGAC 460 0.0 26.844194 3 CGTAGAC 150 1.0622243E-6 25.329906 3 CGTAGCG 85 1.1259908E-7 25.143415 52-53 GTACCCG 95 0.0012233953 25.01252 1 AGAACCG 95 0.0012289046 24.993248 5 GAACCGT 115 1.2847147E-4 24.775915 6 CTTTGCG 785 0.0 24.20148 2 CCGTTCA 245 2.9831426E-10 23.25992 9 GCCGTTC 250 3.8562575E-10 22.793842 8 GAGTACG 1585 0.0 22.787432 1 CGAACTA 230 0.0 22.715195 28-29 TACCTGG 1205 0.0 22.466705 2 CCTCAGG 820 0.0 22.010067 2 GAGTACT 7015 0.0 21.933624 12-13 >>END_MODULE