##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003788_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4115350 Sequences flagged as poor quality 0 Sequence length 121 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.732168345341222 31.0 31.0 34.0 27.0 34.0 2 30.42959335171978 31.0 31.0 34.0 26.0 34.0 3 30.593020034747955 31.0 31.0 34.0 26.0 34.0 4 33.9708498669615 37.0 35.0 37.0 28.0 37.0 5 33.90695566598224 37.0 35.0 37.0 28.0 37.0 6 34.04019536612925 37.0 35.0 37.0 30.0 37.0 7 34.00353189886644 37.0 35.0 37.0 29.0 37.0 8 34.08211209253162 37.0 35.0 37.0 30.0 37.0 9 35.51063870630688 39.0 35.0 39.0 30.0 39.0 10-11 35.425477905888926 39.0 35.0 39.0 30.0 39.0 12-13 35.45410876353166 39.0 35.0 39.0 30.0 39.0 14-15 36.350479910578684 39.0 36.0 41.0 29.0 41.0 16-17 36.343248204891445 39.0 36.0 41.0 29.0 41.0 18-19 36.25815665739245 39.0 36.0 41.0 27.0 41.0 20-21 36.178776288772525 39.0 36.0 41.0 27.0 41.0 22-23 35.99138189947392 39.0 36.0 41.0 27.0 41.0 24-25 35.8827629484734 39.0 35.5 41.0 27.0 41.0 26-27 35.753521085691375 39.0 35.0 40.5 27.0 41.0 28-29 35.6136695542299 39.0 35.0 40.0 27.0 41.0 30-31 35.374874555019616 38.0 35.0 40.0 26.0 41.0 32-33 35.134448831812605 38.0 34.0 40.0 25.0 41.0 34-35 35.07357673101923 38.0 34.0 40.0 25.0 41.0 36-37 35.06396223893472 38.0 34.5 40.0 25.0 41.0 38-39 34.99712794780517 38.0 34.5 40.0 25.0 41.0 40-41 35.15788146816188 39.0 35.0 40.0 25.0 41.0 42-43 35.032113914976854 39.0 35.0 40.0 24.0 41.0 44-45 34.83166996731748 38.0 34.0 40.0 24.0 41.0 46-47 34.57604420037178 38.0 34.0 40.0 23.0 41.0 48-49 34.26801936651803 38.0 33.0 40.0 22.0 41.0 50-51 33.44947537876487 37.5 32.5 39.5 19.0 40.5 52-53 33.60824790115057 38.0 33.0 39.5 20.0 40.5 54-55 33.99343494477991 38.0 33.0 40.0 20.5 41.0 56-57 33.67661511171589 38.0 33.0 40.0 18.0 41.0 58-59 33.4567585989041 38.0 33.0 40.0 17.5 41.0 60-61 32.945355802058145 37.5 32.0 40.0 13.0 41.0 62-63 32.84452148662933 37.0 32.0 40.0 13.0 41.0 64-65 32.50998772886875 36.5 31.0 39.5 12.0 41.0 66-67 32.038548361621736 36.0 31.0 39.0 8.0 41.0 68-69 31.680394012659917 35.5 31.0 39.0 8.0 40.0 70-71 31.200340432769995 35.0 30.5 38.5 7.0 40.0 72-73 30.787235715066764 35.0 30.0 37.0 4.0 40.0 74-75 30.361971642752138 35.0 29.5 37.0 2.0 39.0 76-77 29.964994957901517 34.5 29.0 36.5 2.0 39.0 78-79 29.404395859404424 34.0 29.0 36.0 2.0 38.5 80-81 28.938011347759 34.0 28.0 35.5 2.0 37.0 82-83 28.596109079422163 34.0 27.5 35.0 2.0 37.0 84-85 28.35655521401582 34.0 27.0 35.0 2.0 36.5 86-87 28.25525751151178 34.0 27.0 35.0 2.0 36.0 88-89 27.850428639119393 34.0 26.5 35.0 2.0 36.0 90-91 27.550757894225278 34.0 26.0 35.0 2.0 35.5 92-93 27.342958922084392 33.5 25.5 35.0 2.0 35.0 94-95 26.99742731480919 33.0 25.0 35.0 2.0 35.0 96-97 26.760063542590544 33.0 25.0 35.0 2.0 35.0 98-99 26.518794513224876 33.0 25.0 35.0 2.0 35.0 100-101 26.19020180543575 33.0 24.0 35.0 2.0 35.0 102-103 25.87749960513687 32.5 22.0 35.0 2.0 35.0 104-105 25.522160205085836 32.0 20.0 35.0 2.0 35.0 106-107 25.041897529979224 32.0 19.0 35.0 2.0 35.0 108-109 24.81067102433572 32.0 18.0 34.0 2.0 35.0 110-111 24.50676175780918 32.0 14.0 34.0 2.0 35.0 112-113 23.914005127145927 31.0 6.0 34.0 2.0 35.0 114-115 23.405159463958107 31.0 2.0 34.0 2.0 35.0 116-117 22.758584324541047 31.0 2.0 34.0 2.0 35.0 118-119 22.009947635073566 30.5 2.0 34.0 2.0 35.0 120-121 20.441262833051866 27.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5081396398648117 1101 2 0.687710671106263 1101 3 0.7215041342569641 1101 4 0.9222697797503443 1101 5 1.0136615307812988 1101 6 0.9682555113816775 1101 7 0.9337087760402056 1101 8 0.8447013636808052 1101 9 0.9404767819056445 1101 10-11 1.0140341923978937 1101 12-13 0.8062055161897064 1101 14-15 0.9172359188714765 1101 16-17 0.9948102451444498 1101 18-19 1.0242358018348838 1101 20-21 1.1532526852508838 1101 22-23 1.1780836933661831 1101 24-25 1.0447650396215309 1101 26-27 0.864701324746818 1101 28-29 1.0185880901785183 1101 30-31 1.1022430545506197 1101 32-33 1.095601311632798 1101 34-35 1.0323418917031972 1101 36-37 0.8887966682434367 1101 38-39 0.9293142363093807 1101 40-41 1.063900608543598 1101 42-43 1.054964898441085 1101 44-45 1.0370384310807879 1101 46-47 1.1840955012981809 1101 48-49 1.150409227554178 1101 50-51 0.8464560094409208 1101 52-53 0.9238166775751679 1101 54-55 0.9690236987366916 1101 56-57 0.9384074769494646 1101 58-59 0.9888524010992796 1101 60-61 0.9953176274416009 1101 62-63 0.9740185538799864 1101 64-65 0.8655890800265027 1101 66-67 0.929284747783754 1101 68-69 0.8769920711695072 1101 70-71 0.5954838523826247 1101 72-73 0.582087097101649 1101 74-75 0.7627786089131021 1101 76-77 0.7862623942016178 1101 78-79 0.9592676235182758 1101 80-81 0.8595267587409374 1101 82-83 0.7278820922933882 1101 84-85 0.7801919839497913 1101 86-87 0.8929912798219632 1101 88-89 0.8871698181520529 1101 90-91 0.7785433626980662 1101 92-93 0.8465913366628115 1101 94-95 0.8010134041362207 1101 96-97 0.7937877096868249 1101 98-99 0.7921927589622655 1101 100-101 0.8633007502899375 1101 102-103 0.757768514720798 1101 104-105 0.5058204876251189 1101 106-107 0.5884399983826611 1101 108-109 0.5048410575979609 1101 110-111 0.54512272913367 1101 112-113 0.42597330838998104 1101 114-115 0.334752816150921 1101 116-117 0.3043103553864377 1101 118-119 0.38150018526593854 1101 120-121 0.5027904771990244 1102 1 0.6151906928843687 1102 2 0.7292342820348026 1102 3 0.7552859421417679 1102 4 1.0213456623625063 1102 5 1.191514678432192 1102 6 1.1039377866381912 1102 7 1.1095890139938476 1102 8 0.9801379662225997 1102 9 1.0855189414860718 1102 10-11 1.1083628153393477 1102 12-13 0.9658437041239694 1102 14-15 1.149460694494259 1102 16-17 1.1701420987472488 1102 18-19 1.24434892709872 1102 20-21 1.3266607781762687 1102 22-23 1.354282133221389 1102 24-25 1.268594236137126 1102 26-27 1.2657211343352657 1102 28-29 1.3071959843652365 1102 30-31 1.2887828390535176 1102 32-33 1.2808484551988926 1102 34-35 1.2749351561304252 1102 36-37 1.3277597382924498 1102 38-39 1.4468711492818827 1102 40-41 1.458536040079231 1102 42-43 1.417936417778435 1102 44-45 1.528363954811013 1102 46-47 1.4928502229337752 1102 48-49 1.5213659063486134 1102 50-51 1.249338921564508 1102 52-53 1.3638266434618416 1102 54-55 1.3999072200475382 1102 56-57 1.4217823685899873 1102 58-59 1.311213728478947 1102 60-61 1.3664271097424532 1102 62-63 1.2156102351152285 1102 64-65 1.2726355438739603 1102 66-67 1.3006467793190453 1102 68-69 1.3435928149338814 1102 70-71 1.0261028810300665 1102 72-73 0.986664962757331 1102 74-75 1.1260183849035954 1102 76-77 1.1433099727691136 1102 78-79 1.1489871831198926 1102 80-81 1.1728592881246698 1102 82-83 1.0533942410045682 1102 84-85 1.112403382932122 1102 86-87 0.9888819585040522 1102 88-89 1.0473970704800806 1102 90-91 1.0196497787949674 1102 92-93 0.9639283602502857 1102 94-95 1.037042053309797 1102 96-97 0.9175969860105795 1102 98-99 1.1845581427647147 1102 100-101 1.1727737425054272 1102 102-103 1.0856044927877377 1102 104-105 1.1361070112099867 1102 106-107 1.1012126632468622 1102 108-109 1.1059187312901635 1102 110-111 0.9980925459895111 1102 112-113 0.7584541221761434 1102 114-115 0.6370959431925272 1102 116-117 0.671845133203913 1102 118-119 0.8176908597207841 1102 120-121 0.786218252895349 1103 1 0.6574503978572004 1103 2 0.982584694400046 1103 3 0.9199703158585599 1103 4 1.0677655461496798 1103 5 1.1809860944718693 1103 6 1.148266682015425 1103 7 1.149825775682146 1103 8 1.0423303271410091 1103 9 1.1667299149555461 1103 10-11 1.1793633379023518 1103 12-13 1.0469645398948586 1103 14-15 1.1177050507255117 1103 16-17 1.197160800257734 1103 18-19 1.324297825504111 1103 20-21 1.3466200924934881 1103 22-23 1.3650601423329576 1103 24-25 1.2673281844098696 1103 26-27 1.2341112279076185 1103 28-29 1.1700790916768185 1103 30-31 1.2809998464465764 1103 32-33 1.1464337787785084 1103 34-35 1.2228637711970904 1103 36-37 1.1163768934406377 1103 38-39 1.0312535116318955 1103 40-41 1.186199638529331 1103 42-43 1.127188433946671 1103 44-45 1.1759462467004482 1103 46-47 1.0695613074552384 1103 48-49 1.1478706315081695 1103 50-51 0.9911606191813931 1103 52-53 1.1608973008130974 1103 54-55 1.1773883191147547 1103 56-57 1.261268630838849 1103 58-59 1.107903951719976 1103 60-61 1.0576850196200738 1103 62-63 1.166369277016301 1103 64-65 1.1696322352902015 1103 66-67 1.0208261503935532 1103 68-69 1.064822540579467 1103 70-71 0.8959232036052036 1103 72-73 0.7991046132115827 1103 74-75 0.9430468837338672 1103 76-77 1.0103480303193848 1103 78-79 0.9839672968583493 1103 80-81 0.9040453264432067 1103 82-83 0.9649780693588283 1103 84-85 1.0917528656418867 1103 86-87 1.0370536360608895 1103 88-89 1.0100081151170919 1103 90-91 0.9569783036061104 1103 92-93 0.9199743758399848 1103 94-95 0.9332246334042082 1103 96-97 1.0667032972401458 1103 98-99 1.136211197460625 1103 100-101 1.0952011949136065 1103 102-103 1.019216531295978 1103 104-105 1.1008964572048363 1103 106-107 1.1156681284487036 1103 108-109 1.1224184959306136 1103 110-111 1.1285190710987507 1103 112-113 1.041790415914285 1103 114-115 1.1210954707060239 1103 116-117 1.0518144893320702 1103 118-119 0.7989861089927608 1103 120-121 0.7709892530644318 1104 1 0.7472376685758029 1104 2 0.8654874007256055 1104 3 0.8757922698358023 1104 4 1.157981746001596 1104 5 1.2471064340582743 1104 6 1.2360918285722065 1104 7 1.16999134434203 1104 8 1.042667887606207 1104 9 1.1026450102969463 1104 10-11 1.1871150700940163 1104 12-13 0.9691730400299718 1104 14-15 1.2023281571278588 1104 16-17 1.0945775634010815 1104 18-19 1.2111178735453265 1104 20-21 1.218044304125776 1104 22-23 1.2463523499812723 1104 24-25 1.1408152415703867 1104 26-27 1.0915530836211005 1104 28-29 1.1485769864563693 1104 30-31 1.297066882846984 1104 32-33 1.296880672286953 1104 34-35 1.3708529194770662 1104 36-37 1.0886122214311271 1104 38-39 1.22486979862218 1104 40-41 1.4046658291004874 1104 42-43 1.4089322833964246 1104 44-45 1.3675760925132963 1104 46-47 1.357942155373749 1104 48-49 1.2649020246314322 1104 50-51 1.197617657345866 1104 52-53 1.1749819248907372 1104 54-55 1.316894749886231 1104 56-57 1.3523995245855787 1104 58-59 1.4014482804883315 1104 60-61 1.3935023576809797 1104 62-63 1.5007442131661435 1104 64-65 1.542233268173426 1104 66-67 1.4451273167757606 1104 68-69 1.358978859564612 1104 70-71 1.0642195060087225 1104 72-73 1.075465984783353 1104 74-75 1.198617099044494 1104 76-77 1.306756335170153 1104 78-79 1.3375895825254425 1104 80-81 1.369324333261467 1104 82-83 1.3205676231718044 1104 84-85 1.2964288263006978 1104 86-87 1.3035641702208238 1104 88-89 1.4155337396399261 1104 90-91 1.3206979062574398 1104 92-93 1.2220921073185487 1104 94-95 1.2756868477835965 1104 96-97 1.1472861458878754 1104 98-99 1.4233711225584607 1104 100-101 1.3189600774589678 1104 102-103 1.2983493591807154 1104 104-105 1.2436161873406455 1104 106-107 1.1624249292273063 1104 108-109 1.0838796953264023 1104 110-111 1.1467481635733385 1104 112-113 1.1434198214308822 1104 114-115 1.3213365257134164 1104 116-117 1.197127614793768 1104 118-119 1.1020166278545638 1104 120-121 1.0207155067792506 1105 1 0.7798748023838442 1105 2 0.9787998098603197 1105 3 1.0120565851038315 1105 4 1.2716728644638664 1105 5 1.2240261659786427 1105 6 1.2150183595171384 1105 7 1.2722396745277678 1105 8 1.1924681503184758 1105 9 1.2731484885452815 1105 10-11 1.296503810782923 1105 12-13 1.261844758095009 1105 14-15 1.4234297382808805 1105 16-17 1.4172535398667634 1105 18-19 1.3814592359230247 1105 20-21 1.3867868602418483 1105 22-23 1.5332238685446882 1105 24-25 1.4869062876176784 1105 26-27 1.4349449246807282 1105 28-29 1.3761200570182908 1105 30-31 1.4371629616251838 1105 32-33 1.5898633839011822 1105 34-35 1.6434858267670265 1105 36-37 1.4390760641164988 1105 38-39 1.4093034244063531 1105 40-41 1.4699962723955622 1105 42-43 1.5719569455244908 1105 44-45 1.5396536333867346 1105 46-47 1.5207919486084336 1105 48-49 1.545295007641073 1105 50-51 1.29402818740585 1105 52-53 1.5304917020233475 1105 54-55 1.5514277591449215 1105 56-57 1.7901009045180842 1105 58-59 1.7349930205872752 1105 60-61 1.778039123536594 1105 62-63 1.5233650951873727 1105 64-65 1.632737277335643 1105 66-67 1.5793561387180617 1105 68-69 1.4447404770138554 1105 70-71 1.1453898273405407 1105 72-73 1.2245286168187235 1105 74-75 1.3756584767812647 1105 76-77 1.4110646597334728 1105 78-79 1.4330862961269268 1105 80-81 1.667873358098813 1105 82-83 1.426573416648246 1105 84-85 1.4798926332221498 1105 86-87 1.3596853716221062 1105 88-89 1.4381195855035642 1105 90-91 1.450962097599355 1105 92-93 1.3283182716417983 1105 94-95 1.4932326574730084 1105 96-97 1.4589861362953833 1105 98-99 1.455635577000784 1105 100-101 1.4289534777696957 1105 102-103 1.2672347907374615 1105 104-105 1.2500928861818927 1105 106-107 1.2947217028788955 1105 108-109 1.2681378284280385 1105 110-111 1.3643579809611417 1105 112-113 1.1953127720034935 1105 114-115 1.2124767235737934 1105 116-117 1.1935003501618944 1105 118-119 1.106046721661361 1105 120-121 1.0571782008608501 1106 1 0.5475166678475922 1106 2 0.8423065210881262 1106 3 0.811998060729767 1106 4 1.0389409369420974 1106 5 1.1446593493799142 1106 6 1.0639252281086797 1106 7 1.0889214750979193 1106 8 0.9073238984239396 1106 9 1.0655214341043546 1106 10-11 1.0183645584879528 1106 12-13 0.8586465214563148 1106 14-15 1.12175796504917 1106 16-17 1.044735820212317 1106 18-19 1.101732073686854 1106 20-21 1.1487740076074644 1106 22-23 1.196610974134238 1106 24-25 1.0969108710799773 1106 26-27 1.0954108847618542 1106 28-29 1.0489127302969763 1106 30-31 1.1407044709100802 1106 32-33 1.0990145477867515 1106 34-35 1.2526352048880582 1106 36-37 1.0307074980096544 1106 38-39 1.0056357481513913 1106 40-41 1.1074903333756296 1106 42-43 1.1482523824090833 1106 44-45 1.0825841568646695 1106 46-47 1.1830139104818258 1106 48-49 1.238798220393818 1106 50-51 1.1651687038056622 1106 52-53 1.1667890978375155 1106 54-55 1.1836285840599103 1106 56-57 1.3493392461813727 1106 58-59 1.395768716115505 1106 60-61 1.518658718893171 1106 62-63 1.4028404646241768 1106 64-65 1.3395721693347653 1106 66-67 1.2671050634713552 1106 68-69 1.1196073428563942 1106 70-71 0.9917970990819533 1106 72-73 1.1357879569083806 1106 74-75 1.2544536999152491 1106 76-77 1.1889286066627136 1106 78-79 1.3096166422189164 1106 80-81 1.3566298869621782 1106 82-83 1.1543424745438173 1106 84-85 1.1757467643990012 1106 86-87 1.2222494156320032 1106 88-89 1.4112568842803697 1106 90-91 1.2076617585205511 1106 92-93 1.0324444158839583 1106 94-95 1.2393710093097496 1106 96-97 1.0555461450185746 1106 98-99 1.105642774172999 1106 100-101 1.2168642441981916 1106 102-103 0.9833263014293188 1106 104-105 0.846120277104145 1106 106-107 0.8255132213631811 1106 108-109 0.7679306391564786 1106 110-111 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-1.5680020504122645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67248.0 3 12006.0 4 11002.0 5 11765.0 6 13723.0 7 14959.0 8 17335.0 9 19263.0 10 21772.0 11 24351.0 12 26212.0 13 29629.0 14 35046.0 15 36104.0 16 30254.0 17 30835.0 18 32350.0 19 34661.0 20 37645.0 21 41562.0 22 46236.0 23 52208.0 24 59426.0 25 67382.0 26 77556.0 27 89450.0 28 104027.0 29 121251.0 30 141923.0 31 169779.0 32 206846.0 33 258632.0 34 328838.0 35 426989.0 36 570888.0 37 627214.0 38 218230.0 39 753.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.98835059419175 19.093399002738416 14.170920109283472 20.74733029378636 2 13.731649496174064 22.84881295370102 38.1279909296607 25.291546620464217 3 23.935897634220403 24.559870805240177 29.775222823883745 21.729008736655675 4 11.408283169644742 15.818645870049947 42.627484170333005 30.1455867899723 5 11.259084479832369 39.909286531723204 35.47335031050351 13.358278677940921 6 27.329998497408763 38.31615010591795 17.526523681525298 16.827327715147984 7 25.600994549856964 33.3912260183275 23.174067087934947 17.833712343880585 8 23.166111288629203 30.941771938168728 20.17676384677947 25.715352926422597 9 21.930896114483094 21.414391170533502 25.06455084830195 31.590161866681456 10-11 24.910999634294647 24.791019817402496 27.83962354308251 22.45835700522035 12-13 28.7715214792803 23.149439833088316 26.722466638272312 21.356572049359066 14-15 22.8528861364003 25.739875107568068 29.48013417870744 21.92710457732419 16-17 19.82031363696714 29.38394894754499 29.28945144821314 21.506285967274735 18-19 19.139545212489754 32.3908494507947 28.33514646334077 20.13445887337478 20-21 21.748645751061215 27.487838301418655 26.4889926425329 24.274523304987234 22-23 20.02958954142959 27.668687440775823 28.128714259825276 24.173008757969306 24-25 23.66044521192854 27.2789683227565 27.334051500194633 21.726534965120326 26-27 22.304964291029126 25.024067501628323 31.848957058571592 20.82201114877096 28-29 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26.63651860843866 21.87143961026899 54-55 19.63092475821051 27.548951504176838 28.37402435362565 24.446099383987 56-57 22.850465413506416 26.035087521117628 27.646614555546645 23.46783250982931 58-59 25.631144493432846 25.997630734026878 26.765328391041077 21.6058963814992 60-61 22.25877412369237 27.15733993755271 30.83327738028267 19.750608558472248 62-63 20.556819039333778 28.06014578155589 29.569127832178815 21.81390734693151 64-65 19.746599850688497 31.531068632376552 27.58848424285142 21.133847274083532 66-67 21.479383064240565 27.515164572682288 26.272441966423532 24.733010396653615 68-69 19.93365788146749 28.34660965169713 27.627905412842924 24.09182705399246 70-71 23.515292708988905 27.45627368495952 26.68171953572879 22.346714070322786 72-73 22.103620982915338 25.473270799819797 30.788059187312967 21.635049029951897 74-75 19.843366831562843 31.099564547964075 27.794308020692533 21.26276059978055 76-77 21.059474585976115 27.190920619477954 27.35789967904973 24.3917051154962 78-79 21.7191805608377 27.699233411628526 28.453509183147467 22.128076844386307 80-81 23.648954599606945 26.911760044990828 27.512817054255567 21.926468301146663 82-83 21.884001184932682 26.08724392448622 30.517189203056596 21.511565687524502 84-85 21.131301555896066 28.45148664934537 29.086299415498253 21.33091237926032 86-87 21.19550925954344 29.25901554817602 27.823120315751353 21.72235487652919 88-89 20.306018278454758 28.770827440859204 25.99274685538297 24.93040742530307 90-91 21.597598047400627 25.918092483278166 27.276502018406294 25.207807450914917 92-93 23.36458624608336 27.93781348759871 27.98048131171399 20.71711895460394 94-95 22.01547346568587 27.063860113055405 28.5910028735805 22.329663547678223 96-97 18.365441178636914 28.10416484575173 31.320052029882316 22.21034194572904 98-99 20.63069653102661 29.79654483117414 26.963612943668053 22.609145694131193 100-101 19.430410686501162 27.16637009638299 28.586874422633468 24.816344794482383 102-103 21.90664550746726 25.979218549913973 28.428357418944294 23.685778523674472 104-105 24.383684033865087 26.247785557941217 27.331998480648828 22.036531927544868 106-107 21.3683976822154 27.09392162669569 30.616929184544876 20.920751506544036 108-109 19.95764463836864 27.534323220548927 29.92210182345772 22.58593031762471 110-111 19.47950305970002 30.23012581828152 27.760667283503565 22.529703838514898 112-113 21.435264020014724 26.927248041816476 26.176266341924897 25.4612215962439 114-115 19.887656474886814 27.245256441697308 27.63562588850045 25.23146119491543 116-117 22.9159081987471 26.749109392387844 26.652930032431833 23.682052376433223 118-119 22.049305225573004 25.204355892475416 29.896185954223593 22.850152927727986 120-121 20.479561215211152 30.004514620595113 26.968045618534937 22.547878545658794 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10779.0 1 13287.5 2 8754.0 3 1692.0 4 1683.0 5 1859.0 6 1972.0 7 1969.0 8 2103.5 9 2123.5 10 2111.0 11 2161.5 12 2247.5 13 2462.0 14 2672.0 15 2844.0 16 3101.5 17 3380.0 18 3696.0 19 4089.0 20 4494.5 21 5067.0 22 6000.0 23 7325.5 24 9062.5 25 11442.0 26 14909.5 27 19904.0 28 27168.0 29 36312.5 30 46034.0 31 55918.0 32 65639.5 33 73968.0 34 81669.0 35 90258.0 36 98361.5 37 106851.5 38 114606.0 39 118743.0 40 122524.5 41 126234.5 42 130946.0 43 138357.0 44 142212.5 45 147310.5 46 165244.0 47 250683.0 48 434755.5 49 424283.5 50 238625.0 51 150183.0 52 111961.0 53 91244.0 54 78047.0 55 66479.0 56 54661.5 57 43015.0 58 33664.5 59 26677.5 60 20823.5 61 15894.5 62 12045.5 63 8619.5 64 5785.0 65 3860.0 66 2577.5 67 1681.0 68 1057.0 69 711.5 70 474.5 71 334.0 72 238.5 73 176.0 74 130.5 75 89.0 76 71.0 77 55.0 78 44.5 79 33.5 80 25.5 81 20.0 82 18.5 83 15.5 84 13.0 85 12.0 86 11.5 87 10.0 88 8.5 89 7.5 90 6.5 91 11.0 92 9.0 93 4.5 94 7.0 95 7.0 96 3.5 97 2.5 98 4.0 99 4.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1822688228218742 2 0.9848494052753713 3 1.038526492278907 4 1.004872003596292 5 1.0119187918403052 6 1.0299488500370564 7 0.9997934562066411 8 0.9877167191125906 9 0.9784829965859526 10-11 0.9638183872574628 12-13 0.9579258143292795 14-15 0.9584239493603217 16-17 0.9874980256843281 18-19 1.0274460252469413 20-21 1.0107888757942824 22-23 0.9783250513322075 24-25 0.9645716646214781 26-27 0.9679249638548361 28-29 1.003401897772972 30-31 1.0089056823842444 32-33 0.9962336131799239 34-35 1.0049206021359058 36-37 1.0215048537791438 38-39 1.0025514233297288 40-41 1.014907602026559 42-43 1.008772036400306 44-45 0.9814961060420134 46-47 0.9743278214489655 48-49 0.9762353141288104 50-51 0.9524584786227175 52-53 0.9751053980827876 54-55 0.9884821461115094 56-57 1.050177992151336 58-59 1.0190263282588359 60-61 1.0204356859076384 62-63 0.9821157374220905 64-65 0.9714240587070359 66-67 0.9754820367647953 68-69 0.9929775110257937 70-71 0.9650455003827135 72-73 0.9691034784404728 74-75 0.9758465258118995 76-77 0.9935242445964498 78-79 1.1262104073772583 80-81 1.098011104766302 82-83 1.115834619169694 84-85 1.0613192073578188 86-87 1.0333264485402214 88-89 1.029268470482462 90-91 1.0407377258313386 92-93 1.0211282150971361 94-95 1.0171795837535083 96-97 1.028004908452501 98-99 1.074805302100672 100-101 1.0575406709028394 102-103 1.0230478574118846 104-105 1.0021626350128179 106-107 1.0053458393575272 108-109 1.0023327299014664 110-111 1.0045196641840912 112-113 1.0096103612086456 114-115 1.0150533976454008 116-117 1.051441554181297 118-119 1.0488779812166644 120-121 1.0185767917674073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 4115350.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.907611614258016 #Duplication Level Percentage of deduplicated Percentage of total 1 77.40914140577986 34.762596576439414 2 13.458115783960983 12.087436733716709 3 3.8874828905786147 5.2373271542153255 4 1.6747457606239518 3.0083532868290224 5 0.8314653480272928 1.8669561459961772 6 0.5300360343651056 1.4281591423697808 7 0.3650581162743479 1.1475721671596744 8 0.2563406563225073 0.9209317308060122 9 0.18810483685154883 0.7602605060483452 >10 1.2187984247823667 10.377891558506283 >50 0.11116026181913802 3.4597485420786374 >100 0.06190852202696001 5.058820165852553 >500 0.003123720301070293 0.9699278274801234 >1k 0.0030121588575656873 3.0061249675335966 >5k 5.020264762609479E-4 1.802357361623667 >10k+ 0.0010040529525218958 14.105536133344735 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 116162 2.8226517793140316 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 82362 2.001336459839382 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 50987 1.238946869646567 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 49306 1.1980997971010972 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 32731 0.7953394000510285 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 30879 0.7503371523685712 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 25507 0.6198014749656773 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 23679 0.5753824097585868 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 22646 0.5502812640480154 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 21649 0.5260548920504939 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 18148 0.44098314845638886 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 17597 0.4275942507927637 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 13434 0.32643639058646284 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 12877 0.31290169730399603 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 12440 0.3022829163983622 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 11708 0.28449585089968044 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 10545 0.2562358001142066 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 10443 0.25375727459389846 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 9702 0.2357515156669542 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 9412 0.22870472742294096 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 9116 0.221512143560086 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8869 0.21551022391777128 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8237 0.20015308539978374 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 8161 0.19830634089445612 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 6664 0.16193033399346352 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6061 0.1472778742998773 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5729 0.13921051672397244 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 4952 0.12032998408397827 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4744 0.11527573596413429 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 4530 0.11007569222544862 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4513 0.10966260463873059 No Hit ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4347 0.10562892585077818 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 4326 0.10511864118483238 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 4324 0.1050700426452185 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4222 0.1025915171249104 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.42992698069423E-5 0.0 10-11 0.0 0.0 0.0 7.28978094208269E-5 0.0 12-13 0.0 0.0 0.0 1.5794525374512495E-4 0.0 14-15 0.0 0.0 0.0 2.3084306316595185E-4 0.0 16-17 0.0 0.0 0.0 3.158905074902499E-4 0.0 18-19 0.0 0.0 0.0 5.831824753666152E-4 0.0 20-21 0.0 0.0 0.0 8.261751734360383E-4 0.0 22-23 0.0 0.0 0.0 0.001214963490347115 0.0 24-25 0.0 0.0 0.0 0.0016523503468720765 0.0 26-27 0.0 0.0 0.0 0.0022841313618525766 0.0 28-29 0.0 0.0 0.0 0.004361718930346143 0.0 30-31 0.0 0.0 0.0 0.00888138311443741 0.0 32-33 0.0 0.0 0.0 0.01879548519566987 0.0 34-35 0.0 0.0 0.0 0.02873388654670927 0.0 36-37 0.0 0.0 0.0 0.03615731347273014 0.0 38-39 0.0 0.0 0.0 0.04224428055936919 0.0 40-41 0.0 0.0 0.0 0.04745647393295832 0.0 42-43 0.0 0.0 0.0 0.05450326217697158 0.0 44-45 2.42992698069423E-5 0.0 0.0 0.06057807962870716 0.0 46-47 4.85985396138846E-5 0.0 0.0 0.06922861967997862 0.0 48-49 4.85985396138846E-5 0.0 0.0 0.07825579841325768 0.0 50-51 4.85985396138846E-5 0.0 0.0 0.086335305624066 0.0 52-53 4.85985396138846E-5 0.0 0.0 0.1016681448722466 0.0 54-55 4.85985396138846E-5 0.0 0.0 0.13264971387609803 0.0 56-57 4.85985396138846E-5 0.0 0.0 0.20515873498001386 0.0 58-59 4.85985396138846E-5 0.0 0.0 0.2823818144264765 0.0 60-61 9.71970792277692E-5 0.0 0.0 0.3468234779544875 0.0 62-63 9.71970792277692E-5 0.0 0.0 0.43149428359677794 0.0 64-65 1.0934671413124035E-4 0.0 0.0 0.5409989429817634 0.0 66-67 1.2149634903471151E-4 0.0 0.0 0.7885234548701812 0.0 68-69 1.457956188416538E-4 0.0 0.0 1.0438966309062412 0.0 70-71 1.7009488864859612E-4 0.0 0.0 1.1643602609741577 0.0 72-73 1.7009488864859612E-4 0.0 0.0 1.2712162999501864 0.0 74-75 1.7009488864859612E-4 0.0 0.0 1.4027360977802616 0.0 76-77 1.7009488864859612E-4 0.0 0.0 1.5420681108532688 0.0 78-79 1.8224452355206726E-4 0.0 0.0 1.6366773178465988 0.0 80-81 1.943941584555384E-4 0.0 0.0 1.7369604043398497 0.0 82-83 1.943941584555384E-4 0.0 0.0 1.877920468489922 0.0 84-85 1.943941584555384E-4 0.0 0.0 2.2439646688617008 0.0 86-87 1.943941584555384E-4 0.0 0.0 2.8113040203141897 0.0 88-89 1.943941584555384E-4 0.0 0.0 3.4621721117280426 0.0 90-91 2.0654379335900957E-4 0.0 0.0 4.179838895841179 0.0 92-93 2.186934282624807E-4 0.0 0.0 4.934574216044808 0.0 94-95 2.186934282624807E-4 0.0 0.0 5.583644161492947 0.0 96-97 2.4299269806942302E-4 0.0 0.0 6.236808533903556 0.0 98-99 2.4299269806942302E-4 0.0 0.0 6.92152550815848 0.0 100-101 2.4299269806942302E-4 0.0 0.0 7.661268178891224 0.0 102-103 2.6729196787636533E-4 1.214963490347115E-5 0.0 8.531728771550416 0.0 104-105 2.915912376833076E-4 2.42992698069423E-5 0.0 9.300168879925158 0.0 106-107 2.915912376833076E-4 4.85985396138846E-5 0.0 10.056678046824693 0.0 108-109 2.915912376833076E-4 4.85985396138846E-5 0.0 10.792423487674196 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3630 0.0 42.173916 2 ACCTGGG 3935 0.0 37.977978 3 TATAACG 655 0.0 30.753578 2 GTACCTG 5310 0.0 29.612402 1 GTACACG 770 0.0 25.432772 1 AGTACGC 1860 0.0 24.754034 2 TACACGG 725 0.0 24.608923 2 GTACGCA 1885 0.0 23.784086 3 GAGTACG 2215 0.0 23.143112 1 CCTGGGG 3640 0.0 23.051332 4 GTACCCG 435 0.0 21.185379 1 GTCCCGT 360 1.4915713E-10 20.755508 6 TAACGCA 1020 0.0 20.283686 4 TACGCAG 2265 0.0 20.04488 4 GTATAAC 1325 0.0 19.561499 1 GTACTAG 1020 0.0 19.19925 1 ATAACGC 1120 0.0 18.47514 3 ACACGGG 785 0.0 18.30516 3 CCGGTCG 540 0.0 18.08078 9 GTACATG 15380 0.0 18.05079 1 >>END_MODULE