##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003788_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4115350 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.70620773445758 31.0 31.0 34.0 30.0 34.0 2 31.893536394231354 31.0 31.0 34.0 30.0 34.0 3 31.946706841459413 31.0 31.0 34.0 30.0 34.0 4 35.67748381061149 37.0 35.0 37.0 33.0 37.0 5 35.532632461394535 37.0 35.0 37.0 33.0 37.0 6 35.60920577836636 37.0 35.0 37.0 33.0 37.0 7 35.558926215267235 37.0 35.0 37.0 33.0 37.0 8 35.56921841398666 37.0 35.0 37.0 33.0 37.0 9 37.142891613107025 39.0 37.0 39.0 33.0 39.0 10-11 37.04948230405677 39.0 37.0 39.0 33.0 39.0 12-13 37.02396357539456 39.0 37.0 39.0 33.0 39.0 14-15 38.105367587204 40.0 37.0 41.0 33.0 41.0 16-17 38.1449267984497 40.0 37.0 41.0 33.0 41.0 18-19 38.183692638536215 40.0 37.0 41.0 33.0 41.0 20-21 38.12084792301991 40.0 37.0 41.0 33.0 41.0 22-23 38.03790078608138 40.0 37.0 41.0 33.0 41.0 24-25 37.89789349630044 40.0 37.0 41.0 32.5 41.0 26-27 37.689174432308306 39.5 37.0 41.0 32.0 41.0 28-29 37.57003292551059 39.0 37.0 41.0 32.0 41.0 30-31 37.41978604492935 39.0 36.0 41.0 32.0 41.0 32-33 37.180917297435215 39.0 36.0 41.0 31.0 41.0 34-35 37.14679006645851 39.0 36.0 41.0 31.0 41.0 36-37 37.001751491367685 39.0 35.5 40.5 30.5 41.0 38-39 36.78321685883339 39.0 35.0 40.0 30.0 41.0 40-41 36.88948582745088 39.0 36.0 41.0 30.5 41.0 42-43 36.78116114060772 39.0 35.0 41.0 30.0 41.0 44-45 36.57997084087623 39.0 35.0 41.0 30.0 41.0 46-47 36.44214015818825 39.0 35.0 41.0 30.0 41.0 48-49 36.20667100003645 39.0 35.0 41.0 29.0 41.0 50-51 35.98401484685385 39.0 35.0 40.0 28.0 41.0 52-53 35.85032415225922 39.0 35.0 40.0 28.0 41.0 54-55 35.67630505303316 38.5 35.0 40.0 28.0 41.0 56-57 35.37288456631878 38.0 34.0 40.0 27.5 41.0 58-59 35.096350492667696 38.0 34.0 40.0 26.5 41.0 60-61 34.65290023934781 38.0 34.0 40.0 26.0 41.0 62-63 34.460798595502204 37.0 33.5 40.0 26.0 41.0 64-65 34.022143438589666 37.0 33.0 39.5 25.5 41.0 66-67 33.62238886121472 36.0 32.5 39.0 25.0 40.5 68-69 33.27315817609681 36.0 32.0 39.0 24.5 40.0 70-71 32.77199630651099 35.0 32.0 38.5 23.5 40.0 72-73 32.337994338270136 35.0 31.0 37.0 22.5 39.5 74-75 31.98417886692505 35.0 31.0 37.0 22.0 39.0 76-77 30.97437751345572 34.0 30.5 36.0 20.5 38.5 78-79 31.409691034784405 34.0 31.0 36.0 21.5 38.5 80-81 31.181291020204842 34.0 31.0 36.0 20.0 37.5 82-83 30.854877956917395 34.0 31.0 35.0 20.0 37.0 84-85 30.515148650783043 34.0 30.5 35.0 19.5 36.5 86-87 30.32032488123732 34.0 31.0 35.0 18.0 36.0 88-89 29.996004835554693 34.0 30.0 35.0 16.0 36.0 90-91 29.676432502703292 34.0 30.0 35.0 10.5 35.5 92-93 29.443888490650856 34.0 30.0 35.0 6.5 35.0 94-95 29.076352679601975 34.0 29.0 35.0 2.0 35.0 96-97 28.69571543125129 34.0 29.0 35.0 2.0 35.0 98-99 28.38779824316279 34.0 29.0 35.0 2.0 35.0 100-101 27.408322378412528 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.07259920746609438 1101 2 0.11020904530052178 1101 3 0.16969784068543703 1101 4 0.021900356907259777 1101 5 0.07018958020256605 1101 6 0.15276615172358277 1101 7 0.12753454293591915 1101 8 0.06970063181574204 1101 9 0.1709269402825555 1101 10-11 0.21428604273922502 1101 12-13 0.28183167302175605 1101 14-15 0.3275768870631879 1101 16-17 0.2626056410791193 1101 18-19 0.28394498744135177 1101 20-21 0.12792142634221904 1101 22-23 0.1874475595140339 1101 24-25 0.22559768336775932 1101 26-27 0.22196479684230042 1101 28-29 0.24678893065922836 1101 30-31 0.2720670074444911 1101 32-33 0.3502845413226723 1101 34-35 0.16281923427134615 1101 36-37 0.3461960182270971 1101 38-39 0.2159935698412525 1101 40-41 0.23579537199986333 1101 42-43 0.18872739656705306 1101 44-45 0.25046848525201426 1101 46-47 0.1623972147237751 1101 48-49 0.11899054233888506 1101 50-51 0.08325737697790458 1101 52-53 0.07818846119273815 1101 54-55 0.08069739760131256 1101 56-57 0.15794109863255557 1101 58-59 0.1483380345557137 1101 60-61 -0.10189923074545959 1101 62-63 0.021271367132236207 1101 64-65 -0.037745515223910786 1101 66-67 -0.08497070873701773 1101 68-69 0.04584551934237879 1101 70-71 0.009393597812071164 1101 72-73 -0.12664391781569861 1101 74-75 -0.16965930105868665 1101 76-77 0.0016997838476768834 1101 78-79 0.038579484492505145 1101 80-81 -0.05369084370267885 1101 82-83 0.07037787959290753 1101 84-85 -0.033948087597512 1101 86-87 -0.08305248666935228 1101 88-89 -0.017357343457874208 1101 90-91 -0.04821644044807627 1101 92-93 -0.042153082682634846 1101 94-95 -0.09652444816214967 1101 96-97 -0.08038713930192287 1101 98-99 -0.036131045815142926 1101 100-101 0.12032611948878369 1102 1 0.0682533917776702 1102 2 0.166809317118485 1102 3 0.07628821031502042 1102 4 0.04716295309891194 1102 5 0.0649579240065492 1102 6 -0.010710147591197483 1102 7 0.07682708658806803 1102 8 0.08874946002783446 1102 9 0.13265150772775058 1102 10-11 0.1782880411282406 1102 12-13 0.020958230517280185 1102 14-15 0.060790506220392615 1102 16-17 0.15408359191678045 1102 18-19 0.08688904992126112 1102 20-21 0.08188073819223973 1102 22-23 0.14672712608771832 1102 24-25 0.18347111023720686 1102 26-27 0.07764952158751726 1102 28-29 0.08328309850190152 1102 30-31 0.15211502437202284 1102 32-33 0.11278605415701293 1102 34-35 0.2395499746597025 1102 36-37 0.19622404547374828 1102 38-39 0.18376759263713183 1102 40-41 0.17224757135696933 1102 42-43 0.35492889683545314 1102 44-45 0.38665708951250366 1102 46-47 0.15898486848998772 1102 48-49 0.1826431556190471 1102 50-51 0.20459747603452882 1102 52-53 0.10108278399597026 1102 54-55 0.15393283921285672 1102 56-57 0.15040110334622625 1102 58-59 0.07279164801480675 1102 60-61 0.12112635396138671 1102 62-63 -0.06562359323294942 1102 64-65 -0.27980755040736227 1102 66-67 -0.12077819455980432 1102 68-69 -0.06884158308621124 1102 70-71 -0.12428370145001111 1102 72-73 -0.08674150069730047 1102 74-75 -0.1309270504418265 1102 76-77 -0.1599743606119297 1102 78-79 -0.051117404309390935 1102 80-81 -0.04143710273118728 1102 82-83 -0.1999759672914685 1102 84-85 -0.21332485486307107 1102 86-87 -0.1466830227020246 1102 88-89 -0.1790471953361923 1102 90-91 -0.017716252538125588 1102 92-93 -8.636353463558066E-4 1102 94-95 -0.020463046487115832 1102 96-97 0.035377332804312545 1102 98-99 0.018405401476549343 1102 100-101 0.16016454878166186 1103 1 -0.07789987883631966 1103 2 0.023523073368476588 1103 3 -0.058331411542607015 1103 4 0.03154777508873963 1103 5 0.08781519479020972 1103 6 0.01893234155250667 1103 7 0.05247547415424947 1103 8 0.0489382012990589 1103 9 0.14882032562191227 1103 10-11 0.06332779008591416 1103 12-13 0.021800988542338473 1103 14-15 0.07242393976563477 1103 16-17 0.047574707462118226 1103 18-19 -0.016649929920298234 1103 20-21 0.052003846847050283 1103 22-23 0.05820903403768796 1103 24-25 0.04467658118311846 1103 26-27 0.0015235220458720278 1103 28-29 0.07305298520529391 1103 30-31 0.07167493442285178 1103 32-33 -0.023720936227718425 1103 34-35 0.06291506533057145 1103 36-37 0.0140579658803901 1103 38-39 -0.04303955039254248 1103 40-41 -0.069299810142887 1103 42-43 0.20842722634235145 1103 44-45 0.20517425956581548 1103 46-47 0.26014779208562544 1103 48-49 0.27718073744257055 1103 50-51 0.25871802077258366 1103 52-53 0.25536641610926836 1103 54-55 0.16061812550019994 1103 56-57 0.19044558600591444 1103 58-59 0.1986878700669692 1103 60-61 0.09887581887926444 1103 62-63 0.017646849908715012 1103 64-65 0.1202944730955906 1103 66-67 0.07926990833621517 1103 68-69 0.04771434087527382 1103 70-71 0.028342103971567667 1103 72-73 0.15348260931074975 1103 74-75 0.0467827362146096 1103 76-77 0.02871469849777597 1103 78-79 0.07488795505429735 1103 80-81 0.13833266909082553 1103 82-83 0.09557597293722253 1103 84-85 0.02163798248315274 1103 86-87 0.034326510685151135 1103 88-89 -0.16476147473831304 1103 90-91 -0.02740229939912453 1103 92-93 0.09722621131940556 1103 94-95 0.1949547859309959 1103 96-97 0.2860418037637693 1103 98-99 0.06558328144965841 1103 100-101 0.1915541186127463 1104 1 0.06126855980695467 1104 2 0.05421819756223556 1104 3 0.0530528223404616 1104 4 0.03441308213206895 1104 5 0.04628072491698276 1104 6 0.04088607418562873 1104 7 -0.03556536001435262 1104 8 0.021141046648878614 1104 9 -0.01620026614201464 1104 10-11 0.0879991650447991 1104 12-13 0.07514446307838796 1104 14-15 0.014526456562357737 1104 16-17 0.08490118253372003 1104 18-19 -0.0059983936172969265 1104 20-21 0.02864494323653588 1104 22-23 -0.012396032304593518 1104 24-25 0.06900741000773536 1104 26-27 0.08215414139881716 1104 28-29 0.08485123426506647 1104 30-31 0.07249566967156085 1104 32-33 0.16633528212239668 1104 34-35 0.1926909128073433 1104 36-37 0.14019248415743135 1104 38-39 0.3247699512582827 1104 40-41 0.3616019511284847 1104 42-43 0.2771336949275067 1104 44-45 0.45214858971836946 1104 46-47 0.4293232841023098 1104 48-49 0.34066710538117917 1104 50-51 0.39727339004156903 1104 52-53 0.5294370960331634 1104 54-55 0.5291398710993249 1104 56-57 0.6203087301298424 1104 58-59 0.44156366796004676 1104 60-61 0.5433334170545407 1104 62-63 0.4080255996572717 1104 64-65 0.44171387168544385 1104 66-67 0.45859930835629825 1104 68-69 0.338910063210065 1104 70-71 0.1875647422209923 1104 72-73 0.29196920027523277 1104 74-75 0.3056740227516457 1104 76-77 0.2316433233086741 1104 78-79 0.3725208518612284 1104 80-81 0.3381481043812329 1104 82-83 0.3659846398093656 1104 84-85 0.49148973933717244 1104 86-87 0.6168336050665424 1104 88-89 0.4732602871201159 1104 90-91 0.3278251611015506 1104 92-93 0.28497249304627204 1104 94-95 0.3209577549091023 1104 96-97 0.35528612444563734 1104 98-99 0.4029948588815131 1104 100-101 0.5681809485401175 1105 1 0.006165728857698838 1105 2 -0.009762331344429498 1105 3 0.025234182522421378 1105 4 0.06424344197801446 1105 5 -0.012507244435845166 1105 6 0.075847862248132 1105 7 0.028731499304285535 1105 8 0.04791858236566071 1105 9 -0.07185917046884072 1105 10-11 0.03156627699526382 1105 12-13 0.04888037514685095 1105 14-15 0.08571930499989833 1105 16-17 0.1947824394161941 1105 18-19 0.05922025279905796 1105 20-21 0.029340537455311733 1105 22-23 0.04263322617921261 1105 24-25 0.1199287743150208 1105 26-27 0.13529193254201033 1105 28-29 0.06418107955425967 1105 30-31 0.07223769820062387 1105 32-33 0.15345836792140233 1105 34-35 0.2214765990918508 1105 36-37 0.11826608739648492 1105 38-39 0.17212233363382978 1105 40-41 0.1961435409535639 1105 42-43 0.2778207986827681 1105 44-45 0.39573382620931596 1105 46-47 0.2180302329471644 1105 48-49 0.20995090948587602 1105 50-51 0.22558963340029692 1105 52-53 0.32315858193774716 1105 54-55 0.24928507951144496 1105 56-57 0.15860002746712354 1105 58-59 0.3078031383991018 1105 60-61 0.3121559082854546 1105 62-63 0.20333206449718233 1105 64-65 -0.005444047886236092 1105 66-67 0.2096539278892635 1105 68-69 -0.018645361149538076 1105 70-71 0.13490321409836525 1105 72-73 0.012236567397053477 1105 74-75 0.12309571919200124 1105 76-77 0.20992520281589933 1105 78-79 0.27313512476704105 1105 80-81 0.27308776179068417 1105 82-83 0.2253323490337884 1105 84-85 0.2510806362295348 1105 86-87 0.27881400949261703 1105 88-89 0.23063558889544922 1105 90-91 0.34594859261837385 1105 92-93 0.40119006155495995 1105 94-95 0.30972750545997485 1105 96-97 0.3441101099981232 1105 98-99 0.3935548843099319 1105 100-101 0.4034691793299068 1106 1 -0.02212984547664476 1106 2 -0.0018438184858418083 1106 3 -0.027311938905235422 1106 4 -0.05750119044670754 1106 5 0.0014409294305224307 1106 6 -0.08527396044384972 1106 7 -0.12415050819809181 1106 8 4.982815779754901E-4 1106 9 -0.05253557478324922 1106 10-11 0.07099906947643575 1106 12-13 -0.08830841479745288 1106 14-15 -0.037031849253359894 1106 16-17 -0.06407840244732199 1106 18-19 -0.05049218229111574 1106 20-21 -0.0037421033982170115 1106 22-23 0.00447637598645656 1106 24-25 0.02354199828019432 1106 26-27 0.13593622826894602 1106 28-29 0.009536684798568729 1106 30-31 0.03215026067532989 1106 32-33 0.053877035005115204 1106 34-35 0.0855330416973814 1106 36-37 0.029639811115977466 1106 38-39 0.10310963684798224 1106 40-41 0.09424700490385618 1106 42-43 0.1363810097926077 1106 44-45 0.27205468318147297 1106 46-47 0.17125104868440388 1106 48-49 0.17711272897430064 1106 50-51 0.15914796404596387 1106 52-53 0.042551727740551826 1106 54-55 0.13593658431569366 1106 56-57 0.1929130871419673 1106 58-59 0.24269558396574809 1106 60-61 0.06509619664854682 1106 62-63 0.10234119516536566 1106 64-65 0.11457245149578199 1106 66-67 -0.028550826789974337 1106 68-69 -0.1068090297159614 1106 70-71 0.10706341557589383 1106 72-73 0.14393467253854908 1106 74-75 0.15736656988693198 1106 76-77 0.09862769877357636 1106 78-79 0.07334277400825684 1106 80-81 0.09087910386308096 1106 82-83 0.03781469431623208 1106 84-85 0.021988556798728354 1106 86-87 0.11960748690648515 1106 88-89 0.03497931231190776 1106 90-91 0.1761548538335127 1106 92-93 0.2538778130501278 1106 94-95 0.2467546160713141 1106 96-97 0.21584500188902567 1106 98-99 0.2367711842274609 1106 100-101 0.22146155519762445 1107 1 -0.05209955660592769 1107 2 -0.013588969554714225 1107 3 0.01085344625571949 1107 4 0.06208496073897862 1107 5 0.023357075985266817 1107 6 0.05339944647002426 1107 7 0.04086412516472393 1107 8 0.07330578036808788 1107 9 0.15185628539565243 1107 10-11 0.09081642883761987 1107 12-13 0.006148351594418955 1107 14-15 0.1975301094269426 1107 16-17 0.1230606525691158 1107 18-19 0.13368891000650507 1107 20-21 0.08197447506497468 1107 22-23 0.043826875794543696 1107 24-25 0.12134544238762857 1107 26-27 0.0804705160215633 1107 28-29 0.022161871496500396 1107 30-31 0.12644014623679567 1107 32-33 0.1690454073329306 1107 34-35 0.10999953150513875 1107 36-37 0.26025555965994585 1107 38-39 0.2722528758797438 1107 40-41 0.3574386086077723 1107 42-43 0.2466662273843454 1107 44-45 0.2380096900235955 1107 46-47 0.15533726662241776 1107 48-49 0.17382771894059346 1107 50-51 0.15165084274028828 1107 52-53 0.1515598506649738 1107 54-55 0.23618287695374107 1107 56-57 0.22137735065513908 1107 58-59 0.18518147471304047 1107 60-61 -0.011835605414098893 1107 62-63 0.21075441400454764 1107 64-65 0.1469576974572675 1107 66-67 0.15321707743711954 1107 68-69 0.0904892217901363 1107 70-71 0.021744808780503888 1107 72-73 0.1887190338230269 1107 74-75 0.19561467839702118 1107 76-77 0.16477371897867243 1107 78-79 0.16946528487637025 1107 80-81 0.3271794914438111 1107 82-83 0.32429771210572866 1107 84-85 0.3489140356424123 1107 86-87 0.3362262361763797 1107 88-89 0.12270889079178815 1107 90-91 0.09086714606688062 1107 92-93 0.2354815482603172 1107 94-95 0.3365176635447362 1107 96-97 0.33586738999542476 1107 98-99 0.3043764990690079 1107 100-101 0.2963480892705199 1108 1 0.03017036148180452 1108 2 0.068899498759464 1108 3 0.03321403394762612 1108 4 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8606.0 13 11988.0 14 14670.0 15 19131.0 16 23472.0 17 29593.0 18 27520.0 19 21892.0 20 20754.0 21 21556.0 22 22586.0 23 25650.0 24 29969.0 25 36041.0 26 45505.0 27 58077.0 28 73548.0 29 92548.0 30 114106.0 31 141229.0 32 174774.0 33 220360.0 34 287475.0 35 385225.0 36 559326.0 37 785332.0 38 714208.0 39 136485.0 40 39.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.816425240563085 20.063649363044473 14.81013372152103 20.30979167487141 2 14.559937596518235 23.78914038474857 37.2075170923088 24.4434049264244 3 24.457352751705912 24.840323509892723 29.648582881785675 21.05374085661569 4 12.374548944804209 16.539978373649873 41.05334904686114 30.032123634684776 5 11.96240902960866 39.974242774004644 34.93061343506628 13.132734761320423 6 28.0987036339558 39.20463630067916 16.666334576645973 16.030325488719065 7 26.397050068645438 33.394049108824284 22.312294215558822 17.89660660697146 8 24.296985675580448 30.94526589475986 19.65111108411192 25.106637345547767 9 22.368182536114787 21.392226663588758 24.23716087331576 32.002429926980696 10-11 25.23840013607591 25.20042037736766 27.833051866791404 21.728127619765026 12-13 29.27045442920462 23.326208381572453 26.39346913305905 21.00986805616388 14-15 22.81634152688973 26.307028855927395 29.239349579508893 21.637280037673982 16-17 20.27500698604007 29.291093102652265 29.00112991604602 21.43276999526164 18-19 19.617067807112395 32.23244681497321 28.31246431044747 19.838021067466922 20-21 22.333466426208425 27.241227717372947 26.82915419275364 23.596151663664987 22-23 20.593582562843988 27.76643541861567 27.993998080357684 23.645983938182656 24-25 23.865953078110003 27.598138675932788 27.5740216506494 20.96188659530781 26-27 22.852102904461063 25.06523506737838 31.562084003423475 20.52057802473708 28-29 20.012846249999544 31.13955545316143 28.81410715585459 20.033491140984435 30-31 21.59507398440419 27.258558101527687 27.69644370593364 23.449924208134487 32-33 21.93226179863324 27.786910214143717 28.85151103986609 21.42931694735695 34-35 24.49790520704982 26.163764073374434 28.721645934609608 20.61668478496614 36-37 23.095045600155256 25.61246290418955 31.01804599303271 20.274445502622484 38-39 21.456500233308702 28.20220298355584 30.541744147561467 19.799552635573992 40-41 21.368805464947503 29.001951107373113 28.53276433932695 21.09647908835243 42-43 20.174306737329044 29.658926248626173 26.571163505066075 23.59560350897871 44-45 22.18769080659282 26.21013831940135 27.248915990724026 24.353254883281803 46-47 24.18958096632934 28.590893135533506 27.411087827345188 19.808438070791965 48-49 23.387498206390752 27.690204287385228 28.14411955564417 20.778177950579853 50-51 19.391189917267955 28.478921267322814 31.19597032858205 20.93391848682718 52-53 21.647880773363543 30.557268665732597 26.404009212401846 21.39084134850201 54-55 20.367391654841917 27.623457633856614 28.026282841249934 23.982867870051543 56-57 23.167981900023634 26.347944584618542 27.868896680314148 22.615176835043673 58-59 26.14631221898692 26.18133025777262 26.542361134399766 21.12999638884069 60-61 22.396261066772134 27.660402507607696 30.69163338965062 19.251703035969552 62-63 21.042606831775668 27.96600891914725 29.480475407583256 21.51090884149383 64-65 20.248791391020337 31.404575967145533 27.54812704360232 20.798505598231813 66-67 22.093559408053558 27.337587587830892 26.401417447466745 24.1674355566488 68-69 20.628595691355855 28.46371819671681 27.31931890183749 23.588367210089846 70-71 23.72243517973287 27.873267747334562 26.787103434649694 21.61719363828287 72-73 22.664658410818728 25.54537644530696 30.440858489281155 21.349106654593157 74-75 20.32988027846535 31.340910590287585 27.62683242690795 20.702376704339116 76-77 21.704374889285834 27.191387221477175 27.058833963862412 24.045403925374583 78-79 21.870029251631586 27.95412805692672 28.336831638611375 21.839011052830323 80-81 23.954624626638633 27.110591264437527 27.544251765842525 21.390532343081308 82-83 22.330647717631642 26.260549150558056 30.062392482527784 21.346410649282515 84-85 21.027436255883806 28.49148341040711 29.195523265378327 21.285557068330757 86-87 21.678808703386 29.027162845853642 27.247678108855506 22.046350341904848 88-89 20.657398263668846 29.06167746851782 25.986126554669546 24.294797713143787 90-91 22.09862442029106 25.6732314465499 27.23240564918471 24.995738483974332 92-93 23.41384323137924 28.260523299150194 27.58167171182401 20.743961757646556 94-95 22.259742415161305 27.433017383690068 28.37803539042621 21.929204810722418 96-97 18.69708954610022 28.153273205086037 31.005017194397723 22.14462005441602 98-99 21.035994915723165 30.1105372135553 26.467206450873682 22.386261419847852 100-101 19.697415771662808 27.424997621517953 27.856635916306104 25.020950690513132 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 242.0 1 179.5 2 218.5 3 347.5 4 295.5 5 242.0 6 390.0 7 748.5 8 1181.5 9 1495.0 10 1611.5 11 1644.0 12 1780.5 13 2068.5 14 2434.5 15 2754.5 16 3056.0 17 3377.5 18 3816.5 19 4244.5 20 4600.5 21 5145.5 22 5947.5 23 7325.0 24 9047.0 25 11298.5 26 14839.0 27 19517.5 28 26183.5 29 35289.0 30 45388.5 31 55011.0 32 64304.0 33 72934.5 34 80953.5 35 89458.0 36 97444.5 37 105985.0 38 114072.5 39 117886.5 40 120712.0 41 124717.5 42 129858.0 43 137094.5 44 140423.5 45 143886.5 46 158024.5 47 256351.0 48 467576.0 49 451399.0 50 243440.5 51 149357.0 52 111060.5 53 91060.0 54 78897.5 55 67673.5 56 55148.0 57 43238.0 58 33721.0 59 26809.0 60 20931.0 61 15665.0 62 11720.0 63 8208.0 64 5265.5 65 3371.0 66 2093.0 67 1253.0 68 756.0 69 413.5 70 233.0 71 156.5 72 80.0 73 37.5 74 30.0 75 22.5 76 13.5 77 5.5 78 2.0 79 3.0 80 2.5 81 0.5 82 0.0 83 0.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04558543015782376 2 0.004738357612353749 3 0.04179474406794076 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.214963490347115E-5 14-15 0.001044868601698519 16-17 0.0 18-19 0.0 20-21 0.010424386747178247 22-23 0.0 24-25 0.0 26-27 0.005637430595210614 28-29 0.012890762632582892 30-31 0.011238412285710814 32-33 0.00696174079968897 34-35 0.00645145613374318 36-37 0.020302039923700294 38-39 0.041345207576512326 40-41 0.0060991167215425175 42-43 0.01160290133281495 44-45 0.01014494514439841 46-47 0.004361718930346143 48-49 0.0010813175064089324 50-51 0.0018588941402310861 52-53 6.803795545943845E-4 54-55 0.0010084196969881055 56-57 0.010387937842467833 58-59 0.007970160496677075 60-61 0.006621551022391777 62-63 0.006572952482777892 64-65 0.0648183022100186 66-67 0.029013328149489107 68-69 0.021310459620688397 70-71 0.013340299124011324 72-73 1.0934671413124037E-4 74-75 2.0654379335900957E-4 76-77 5.588832055596729E-4 78-79 5.831824753666152E-4 80-81 8.261751734360383E-4 82-83 7.411277291117401E-4 84-85 7.046788244013268E-4 86-87 0.004130875867180191 88-89 1.457956188416538E-4 90-91 7.411277291117401E-4 92-93 4.252372216214903E-4 94-95 0.0020532882986866244 96-97 0.004896302866098873 98-99 0.007544923275055585 100-101 0.008030908671194431 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4115350.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.96377017152478 #Duplication Level Percentage of deduplicated Percentage of total 1 74.48229483919829 30.510756076406647 2 14.83600157753631 12.154771177731527 3 4.513703455087609 5.546949328698783 4 1.9508839547566574 3.1966224781586825 5 1.0374051859700764 2.1248013806413066 6 0.6135309477668349 1.507952444246304 7 0.3970936415879074 1.138651686940661 8 0.32436021133977083 1.062961372008766 9 0.2206884200746747 0.8136206747510301 >10 1.4023711889548378 10.95794031776545 >50 0.13385582303800397 3.821675994447793 >100 0.07864445967329943 5.86121537011485 >500 0.00419372974688168 1.1456495140396286 >1k 0.003235162946086932 2.7234024286525536 >5k 4.7928339942028623E-4 1.471095257145741 >10k+ 0.0012581189234782512 15.961934498250313 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 140968 3.4254194661450423 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 62581 1.520672603788256 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 48748 1.1845408045488233 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 46381 1.1270244329157908 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 39012 0.947963113708433 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 35938 0.8732671583218924 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 33411 0.8118629035197493 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 32044 0.7786458016936592 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 28978 0.704144240465574 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 24424 0.5934853657647587 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 24080 0.5851264169511706 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 23930 0.5814815264801293 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 16133 0.39202011979540013 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 13098 0.3182718359313303 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 13000 0.31589050749024994 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 12792 0.31083625937040593 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 12630 0.30689977766168125 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 11765 0.28588090927867615 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 10364 0.25183763227915 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 10121 0.24593290971606305 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10006 0.24313849368826465 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 9793 0.23796274921938596 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9247 0.22469534790479545 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8011 0.1946614504234148 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 7406 0.17996039219021467 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6678 0.1622705237707607 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 6440 0.15648729755670843 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 6356 0.15444615889292526 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6012 0.14608721007933712 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 4944 0.12013558992552274 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 4.85985396138846E-5 0.0 0.0 2.42992698069423E-5 0.0 10-11 4.85985396138846E-5 0.0 0.0 7.28978094208269E-5 0.0 12-13 4.85985396138846E-5 0.0 0.0 1.5794525374512495E-4 0.0 14-15 4.85985396138846E-5 0.0 0.0 2.186934282624807E-4 0.0 16-17 4.85985396138846E-5 0.0 0.0 3.037408725867788E-4 0.0 18-19 6.074817451735575E-5 0.0 0.0 5.831824753666152E-4 0.0 20-21 7.28978094208269E-5 0.0 0.0 8.261751734360383E-4 0.0 22-23 7.28978094208269E-5 0.0 0.0 0.0012514123950575285 0.0 24-25 7.28978094208269E-5 0.0 0.0 0.0017252481562929035 0.0 26-27 7.28978094208269E-5 0.0 0.0 0.002357029171273403 0.0 28-29 7.28978094208269E-5 0.0 0.0 0.004398167835056557 0.0 30-31 7.28978094208269E-5 0.0 0.0 0.009148675082313776 0.0 32-33 7.28978094208269E-5 0.0 0.0 0.01917212387767748 0.0 34-35 7.28978094208269E-5 0.0 0.0 0.029195572673041174 0.0 36-37 7.28978094208269E-5 0.0 0.0 0.03683769302732452 0.0 38-39 7.28978094208269E-5 0.0 0.0 0.043131203907322585 0.0 40-41 7.28978094208269E-5 0.0 0.0 0.048404145455429065 0.0 42-43 7.28978094208269E-5 0.0 0.0 0.0556331782229944 0.0 44-45 8.504744432429804E-5 0.0 0.0 0.061744444579440394 0.0 46-47 9.71970792277692E-5 2.42992698069423E-5 0.0 0.07063797732878127 0.0 48-49 9.71970792277692E-5 2.42992698069423E-5 0.0 0.07985955022051588 0.0 50-51 9.71970792277692E-5 2.42992698069423E-5 0.0 0.08804840414545542 0.0 52-53 9.71970792277692E-5 2.42992698069423E-5 0.0 0.10391582732938875 0.0 54-55 1.0934671413124035E-4 3.644890471041345E-5 0.0 0.1362460058075255 0.0 56-57 1.2149634903471151E-4 6.074817451735575E-5 0.0 0.21099055973368 0.0 58-59 1.2149634903471151E-4 7.28978094208269E-5 0.0 0.2908501099541959 0.0 60-61 1.2149634903471151E-4 7.28978094208269E-5 0.0 0.35783104717703235 0.0 62-63 1.2149634903471151E-4 7.28978094208269E-5 0.0 0.444725236006658 0.0 64-65 1.2149634903471151E-4 7.28978094208269E-5 0.0 0.557364501196739 0.0 66-67 1.2149634903471151E-4 7.28978094208269E-5 0.0 0.8133208597081658 0.0 68-69 1.3364598393818267E-4 7.28978094208269E-5 0.0 1.077794112286926 0.0 70-71 1.457956188416538E-4 7.28978094208269E-5 0.0 1.2035792824425626 0.0 72-73 1.457956188416538E-4 7.28978094208269E-5 0.0 1.316740981933493 0.0 74-75 1.457956188416538E-4 7.28978094208269E-5 0.0 1.4546879366275043 0.0 76-77 1.457956188416538E-4 7.28978094208269E-5 0.0 1.6012368328331732 0.0 78-79 1.457956188416538E-4 7.28978094208269E-5 0.0 1.7006451456133744 0.0 80-81 1.457956188416538E-4 7.28978094208269E-5 0.0 1.8051441554181298 0.0 82-83 1.457956188416538E-4 7.28978094208269E-5 0.0 1.9520453910359994 0.0 84-85 1.5794525374512495E-4 7.28978094208269E-5 0.0 2.3318794270232184 0.0 86-87 1.7009488864859612E-4 7.28978094208269E-5 0.0 2.920274095763422 0.0 88-89 1.7009488864859612E-4 7.28978094208269E-5 0.0 3.595125566476727 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3090 0.0 36.285187 2 ACCTGGG 3320 0.0 32.339672 3 TATAACG 545 0.0 29.638638 2 GTCGCGC 65 0.005852895 29.223518 8 GTACCTG 4135 0.0 27.933014 1 AGTACGC 1790 0.0 23.62179 2 TACGCAG 1810 0.0 23.35056 4 GTACGCA 1820 0.0 23.231857 3 TAACGCA 720 0.0 23.084549 4 GAGTACG 1985 0.0 22.987818 1 ATAACGC 850 0.0 22.915546 3 TGTACCG 250 9.5333235E-9 20.894815 5 GTACCCG 455 0.0 20.893232 1 GTATAAC 1120 0.0 19.946508 1 CCTGGGG 3095 0.0 19.79315 4 GGCGTCG 465 0.0 18.382536 8 ACGCAGT 2310 0.0 18.296324 5 AGTACTT 11325 0.0 17.863768 12-13 CCGGTCG 455 0.0 17.743496 9 CGCAGTG 2425 0.0 17.624493 6 >>END_MODULE