##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003787_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3152816 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.847892804400892 31.0 31.0 34.0 28.0 34.0 2 30.580294568411222 31.0 31.0 34.0 26.0 34.0 3 30.73768783208408 31.0 31.0 34.0 27.0 34.0 4 34.14380350772135 37.0 35.0 37.0 30.0 37.0 5 34.07220116873297 37.0 35.0 37.0 30.0 37.0 6 34.19104254736084 37.0 35.0 37.0 30.0 37.0 7 34.15108271462718 37.0 35.0 37.0 30.0 37.0 8 34.221645030981826 37.0 35.0 37.0 30.0 37.0 9 35.68057063907313 39.0 35.0 39.0 30.0 39.0 10-11 35.59980347727238 39.0 35.0 39.0 30.0 39.0 12-13 35.63274862852764 39.0 35.0 39.0 30.0 39.0 14-15 36.60126819960315 39.0 36.0 41.0 30.0 41.0 16-17 36.57616143790186 39.0 36.0 41.0 30.0 41.0 18-19 36.46961811282358 39.5 36.0 41.0 29.0 41.0 20-21 36.39889324337354 39.0 36.0 41.0 28.0 41.0 22-23 36.2138762617292 39.0 36.0 41.0 27.0 41.0 24-25 36.140688831825265 39.0 36.0 41.0 27.0 41.0 26-27 36.0312009961888 39.0 36.0 41.0 27.0 41.0 28-29 35.87806234172879 39.0 36.0 40.5 27.0 41.0 30-31 35.63589866963375 39.0 35.0 40.0 27.0 41.0 32-33 35.40301543128429 38.0 35.0 40.0 26.0 41.0 34-35 35.34601908262328 38.0 35.0 40.0 25.5 41.0 36-37 35.367375704766786 38.5 35.0 40.0 25.5 41.0 38-39 35.31532905821335 38.5 35.0 40.0 25.0 41.0 40-41 35.44265412253681 39.0 35.0 41.0 25.5 41.0 42-43 35.33858033580139 39.0 35.0 41.0 25.0 41.0 44-45 35.14526902299404 39.0 35.0 41.0 24.5 41.0 46-47 34.92298725964344 39.0 34.5 40.5 24.0 41.0 48-49 34.66425427300547 38.5 34.0 40.0 23.0 41.0 50-51 33.8701016805294 37.5 33.5 39.5 22.5 40.5 52-53 34.0257197058122 38.0 33.5 40.0 22.5 40.5 54-55 34.42173012950962 38.0 34.0 40.0 23.0 41.0 56-57 34.13880147144648 38.0 33.5 40.0 21.0 41.0 58-59 33.9479763487625 38.0 33.0 40.0 20.0 41.0 60-61 33.49714429893784 38.0 33.0 40.0 18.0 41.0 62-63 33.39901408772349 37.5 33.0 40.0 18.0 41.0 64-65 33.08705408117696 37.0 32.0 40.0 17.0 41.0 66-67 32.66851665304921 36.5 32.0 39.0 13.0 41.0 68-69 32.336833960497536 36.0 31.0 39.0 13.0 41.0 70-71 31.910749945445595 35.5 31.0 39.0 9.5 40.5 72-73 31.5260413230585 35.0 31.0 38.0 8.0 40.0 74-75 31.096723849409543 35.0 31.0 37.0 7.0 40.0 76-77 30.69217201384413 35.0 30.0 37.0 6.5 39.0 78-79 30.145808064917205 34.5 29.5 36.0 2.0 39.0 80-81 29.68812737565402 34.0 29.0 36.0 2.0 38.5 82-83 29.35983990185282 34.0 29.0 35.5 2.0 37.0 84-85 29.10809178207672 34.0 29.0 35.0 2.0 37.0 86-87 28.955598106581544 34.0 29.0 35.0 2.0 36.5 88-89 28.56715155594237 34.0 28.5 35.0 2.0 36.0 90-91 28.28244559784015 34.0 27.5 35.0 2.0 36.0 92-93 28.078626218593158 34.0 27.0 35.0 2.0 35.5 94-95 27.758806095883806 34.0 26.5 35.0 2.0 35.0 96-97 27.540725656048433 34.0 26.0 35.0 2.0 35.0 98-99 27.30391339044207 34.0 25.0 35.0 2.0 35.0 100-101 26.996828866638587 33.5 25.0 35.0 2.0 35.0 102-103 26.749961780199037 33.0 25.0 35.0 2.0 35.0 104-105 26.439357228585493 33.0 24.5 35.0 2.0 35.0 106-107 26.033140214969727 33.0 23.5 35.0 2.0 35.0 108-109 25.811500258816245 32.5 23.0 35.0 2.0 35.0 110-111 25.528707669588076 32.0 19.5 35.0 2.0 35.0 112-113 25.02356956447823 32.0 18.5 35.0 2.0 35.0 114-115 24.56829133067074 32.0 13.5 34.0 2.0 35.0 116-117 23.994548365651532 32.0 3.5 34.0 2.0 35.0 118-119 23.290405307509225 31.0 2.0 34.0 2.0 35.0 120-121 21.730046409305203 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7234714606363077 1101 2 0.7050059038623928 1101 3 0.8510207392494209 1101 4 1.0618973763100428 1101 5 1.119581985013184 1101 6 1.1279335736066187 1101 7 1.11195694083181 1101 8 1.0398732907897923 1101 9 1.144010125900607 1101 10-11 1.0446580236761633 1101 12-13 0.810691685044219 1101 14-15 1.008075344851754 1101 16-17 1.041604994676753 1101 18-19 1.1836650520049687 1101 20-21 1.178067373213942 1101 22-23 1.2301495327192598 1101 24-25 1.114680119141319 1101 26-27 1.053547815102256 1101 28-29 1.2336246456304991 1101 30-31 1.2685537490423329 1101 32-33 1.1747674154250731 1101 34-35 1.2034562071481858 1101 36-37 1.0404517051201623 1101 38-39 1.0915794381200499 1101 40-41 1.1413113312015852 1101 42-43 1.265158067224128 1101 44-45 1.1619225719919157 1101 46-47 1.2028968808226495 1101 48-49 1.262831397547906 1101 50-51 0.9696775207062345 1101 52-53 0.9859803730340744 1101 54-55 1.1746263956002423 1101 56-57 1.106548236238858 1101 58-59 0.9639472676729994 1101 60-61 1.15720260141061 1101 62-63 1.1744151685980881 1101 64-65 1.1581018161479122 1101 66-67 1.2033832179862003 1101 68-69 1.0591505037411082 1101 70-71 0.7026775547711246 1101 72-73 0.6750840298515648 1101 74-75 0.7417361646648715 1101 76-77 0.8368526178118643 1101 78-79 0.86162777064715 1101 80-81 0.891287374392828 1101 82-83 1.014092969450509 1101 84-85 0.9397687259906569 1101 86-87 0.9758851101294006 1101 88-89 1.0038342878211637 1101 90-91 1.0254071536401739 1101 92-93 1.1790153602259856 1101 94-95 0.988894496285269 1101 96-97 0.9579344420988853 1101 98-99 1.2124275243261593 1101 100-101 1.0052214338556311 1101 102-103 0.9854470326289153 1101 104-105 0.8487161099840286 1101 106-107 0.8048563181210859 1101 108-109 0.8784880735269809 1101 110-111 0.8834472366726374 1101 112-113 0.815974186927825 1101 114-115 0.7712159268708199 1101 116-117 0.5954302856534568 1101 118-119 0.5415151376498493 1101 120-121 0.48232429381852526 1102 1 0.8116760535999887 1102 2 0.9116048388029689 1102 3 0.9573069013515578 1102 4 1.226432491286893 1102 5 1.2398709869737985 1102 6 1.3069533641590638 1102 7 1.230509723749833 1102 8 1.0801728270599185 1102 9 1.2026259323146746 1102 10-11 1.2704642518261409 1102 12-13 1.03911207030675 1102 14-15 1.24737261783212 1102 16-17 1.3545003939331295 1102 18-19 1.4062838699810811 1102 20-21 1.349720962582147 1102 22-23 1.4839807424347882 1102 24-25 1.392382031575579 1102 26-27 1.437857981835883 1102 28-29 1.5211630998271488 1102 30-31 1.4121378323353753 1102 32-33 1.4856842567853121 1102 34-35 1.5139982018722762 1102 36-37 1.4221504383149721 1102 38-39 1.4672714478770672 1102 40-41 1.4560441953934244 1102 42-43 1.6136236353906241 1102 44-45 1.5751655584639295 1102 46-47 1.5494486647812025 1102 48-49 1.569995864810366 1102 50-51 1.149032310827046 1102 52-53 1.3308370875965565 1102 54-55 1.4711417487997949 1102 56-57 1.5878982691052812 1102 58-59 1.440314936863473 1102 60-61 1.475526852809729 1102 62-63 1.2654381867038182 1102 64-65 1.3515080559974209 1102 66-67 1.481678158995365 1102 68-69 1.346878483144259 1102 70-71 0.9357483829637125 1102 72-73 0.8897450982716428 1102 74-75 1.075492066382708 1102 76-77 1.1672331827686833 1102 78-79 1.2552613223282059 1102 80-81 1.0856709627533156 1102 82-83 1.193332540561066 1102 84-85 1.1121204383547472 1102 86-87 1.1190877746844663 1102 88-89 1.3247545786467363 1102 90-91 1.1208344272943194 1102 92-93 1.0408727404062077 1102 94-95 1.1098336626609253 1102 96-97 0.971734790171606 1102 98-99 1.1247023395464808 1102 100-101 1.1212571609852766 1102 102-103 1.0118532640708686 1102 104-105 0.8263314719819057 1102 106-107 0.7808606270515561 1102 108-109 1.0171081476626576 1102 110-111 0.7821437342870823 1102 112-113 0.9819407175223809 1102 114-115 0.7904621790261466 1102 116-117 0.781341961104836 1102 118-119 0.6392733759571065 1102 120-121 0.6202968679047629 1103 1 0.7408639457965833 1103 2 0.9638843749844384 1103 3 0.9096975493212369 1103 4 1.1186434223018722 1103 5 1.2027094170033052 1103 6 1.2314440679415313 1103 7 1.1717513152377137 1103 8 1.0585643981930346 1103 9 1.1840020719453506 1103 10-11 1.1538492099020345 1103 12-13 1.0459335144158715 1103 14-15 1.168587083179375 1103 16-17 1.20967043774575 1103 18-19 1.3337643997282527 1103 20-21 1.2415227073204065 1103 22-23 1.3456418463427724 1103 24-25 1.2696055966425348 1103 26-27 1.2628640249358867 1103 28-29 1.2724693713043536 1103 30-31 1.3032419470322836 1103 32-33 1.2951935570654314 1103 34-35 1.3096958372905831 1103 36-37 1.2151546374778661 1103 38-39 1.100078233928869 1103 40-41 1.3723232732193438 1103 42-43 1.3671896618292294 1103 44-45 1.3637475108458545 1103 46-47 1.2633827091391723 1103 48-49 1.3243146052072774 1103 50-51 1.1948534668508657 1103 52-53 1.158926575422477 1103 54-55 1.1942305967680653 1103 56-57 1.340686548872796 1103 58-59 1.3358050935108992 1103 60-61 1.3171836527639371 1103 62-63 1.3094929532609214 1103 64-65 1.2434729614484468 1103 66-67 1.215553118981859 1103 68-69 1.3086920058037634 1103 70-71 1.0997906176309975 1103 72-73 1.016712989127587 1103 74-75 1.2467581803700867 1103 76-77 1.3207330009678593 1103 78-79 1.2962650851213304 1103 80-81 1.3418312504992684 1103 82-83 1.3033563915386388 1103 84-85 1.2279052186804442 1103 86-87 1.4053544699804306 1103 88-89 1.4713679513250426 1103 90-91 1.4808427149743189 1103 92-93 1.4504500412440926 1103 94-95 1.462250108065895 1103 96-97 1.5657060406685268 1103 98-99 1.432782803647001 1103 100-101 1.539672383551185 1103 102-103 1.3882376656916513 1103 104-105 1.3559849606877314 1103 106-107 1.443272748189809 1103 108-109 1.4061891531628028 1103 110-111 1.3680955550973088 1103 112-113 1.4783238191149266 1103 114-115 1.427929351222172 1103 116-117 1.33376804918246 1103 118-119 1.1775348916920443 1103 120-121 1.259118913276943 1104 1 0.7967202924381205 1104 2 0.7920646943190128 1104 3 0.8540530197469458 1104 4 0.9993108948499412 1104 5 1.269004090117953 1104 6 1.226250248349686 1104 7 1.188507765834693 1104 8 1.0173351709719114 1104 9 1.121513828257605 1104 10-11 1.1579795845857248 1104 12-13 0.967682221250584 1104 14-15 1.240979983610707 1104 16-17 1.1558598928829653 1104 18-19 1.1231611535561257 1104 20-21 1.2710522967231341 1104 22-23 1.4104024151657626 1104 24-25 1.2278669389117098 1104 26-27 1.2129278606941867 1104 28-29 1.1240711129010208 1104 30-31 1.3584177935623316 1104 32-33 1.3442986706456495 1104 34-35 1.4031374484395585 1104 36-37 1.1765309810688578 1104 38-39 1.2532152888718002 1104 40-41 1.2271401119089163 1104 42-43 1.265113191915276 1104 44-45 1.2912955527470373 1104 46-47 1.3743590416626645 1104 48-49 1.5093639590489687 1104 50-51 1.1317337246399077 1104 52-53 1.306607887693147 1104 54-55 1.5049579221695382 1104 56-57 1.4772635083376073 1104 58-59 1.4107958919077817 1104 60-61 1.5199032816027724 1104 62-63 1.459228960837045 1104 64-65 1.3933765509688243 1104 66-67 1.5164247892280258 1104 68-69 1.4404822715655854 1104 70-71 1.1550431344990564 1104 72-73 1.1982071992673191 1104 74-75 1.2609517258320508 1104 76-77 1.303723637072416 1104 78-79 1.326573875343609 1104 80-81 1.2178868191141632 1104 82-83 1.1486683752926652 1104 84-85 1.1687326328776741 1104 86-87 1.163860808565481 1104 88-89 1.1867090058671224 1104 90-91 1.2076780424339724 1104 92-93 1.174795064972951 1104 94-95 1.222781322484522 1104 96-97 1.1230513264367197 1104 98-99 1.204479986154496 1104 100-101 1.2737858709971306 1104 102-103 1.3363519008913691 1104 104-105 1.4443570872357618 1104 106-107 1.3643575212862977 1104 108-109 1.1634156958176725 1104 110-111 1.1341218064630212 1104 112-113 1.1042828958715276 1104 114-115 1.0156717812033769 1104 116-117 1.0268351789170467 1104 118-119 1.0501663944004456 1104 120-121 1.1742281083465116 1105 1 0.661047458968973 1105 2 0.934031156507146 1105 3 0.9046218304895355 1105 4 0.9960489284052869 1105 5 1.0777486989066958 1105 6 1.1316310789311004 1105 7 1.1491586376472398 1105 8 1.1140000026591323 1105 9 1.2096644210962708 1105 10-11 1.196856539896629 1105 12-13 1.0873223328927537 1105 14-15 1.207915879250585 1105 16-17 1.2770055317156022 1105 18-19 1.3220755560401258 1105 20-21 1.1572971088504005 1105 22-23 1.3337344747559001 1105 24-25 1.2633265464541097 1105 26-27 1.2428754048361839 1105 28-29 1.2763535247874813 1105 30-31 1.2428972326943963 1105 32-33 1.460967244638617 1105 34-35 1.3778456987658316 1105 36-37 1.256131874136031 1105 38-39 1.3724140298610479 1105 40-41 1.4171538775432353 1105 42-43 1.4141540260175702 1105 44-45 1.4158953156685712 1105 46-47 1.371488734808942 1105 48-49 1.5142021552970633 1105 50-51 1.308374940558238 1105 52-53 1.4745641371354736 1105 54-55 1.3955904617262789 1105 56-57 1.5772817814005293 1105 58-59 1.6289464474768636 1105 60-61 1.6847759653796928 1105 62-63 1.477340942375875 1105 64-65 1.4430207469200766 1105 66-67 1.3878182160554289 1105 68-69 1.4226095827463183 1105 70-71 1.109129515334235 1105 72-73 1.1171708239239173 1105 74-75 1.2042796353432266 1105 76-77 1.3719454652600227 1105 78-79 1.4593086589903272 1105 80-81 1.373715761760426 1105 82-83 1.2741726931944157 1105 84-85 1.3332227004211425 1105 86-87 1.3124950204308483 1105 88-89 1.3100474122499577 1105 90-91 1.3110977140713054 1105 92-93 1.2405372593496686 1105 94-95 1.317456012637173 1105 96-97 1.2891187747563997 1105 98-99 1.2007900222643428 1105 100-101 1.3336190807927224 1105 102-103 1.1401744020363118 1105 104-105 1.067560062489406 1105 106-107 1.2736753364374422 1105 108-109 1.2509021773684559 1105 110-111 1.1262534825709523 1105 112-113 0.8876876881713649 1105 114-115 0.9788328150093157 1105 116-117 1.0457323431174501 1105 118-119 1.1069875632670048 1105 120-121 1.1033868853688844 1106 1 0.5651642030090152 1106 2 0.8365645083222262 1106 3 0.8021288615062971 1106 4 0.9626784476277663 1106 5 1.006536193559917 1106 6 0.9247623401230811 1106 7 1.0829458341903262 1106 8 1.0298078578557366 1106 9 1.1500440175514584 1106 10-11 1.0304725097700924 1106 12-13 0.8698756618740049 1106 14-15 1.049401145089611 1106 16-17 1.152320833227222 1106 18-19 1.1847360012402817 1106 20-21 1.212747920083281 1106 22-23 1.300282405941907 1106 24-25 1.0957153954687442 1106 26-27 1.17072079572349 1106 28-29 1.251130540030914 1106 30-31 1.2448360306299335 1106 32-33 1.1997756978949994 1106 34-35 1.302122857437574 1106 36-37 1.0085654753789441 1106 38-39 1.0318406239468274 1106 40-41 1.1079039475260757 1106 42-43 1.124673558940117 1106 44-45 0.9618164494696018 1106 46-47 0.8603957375404079 1106 48-49 0.9684550723669361 1106 50-51 1.093004436191599 1106 52-53 1.1388478704611913 1106 54-55 1.1719980690640455 1106 56-57 1.1605055281504022 1106 58-59 1.1449869908878085 1106 60-61 1.2380590127815836 1106 62-63 1.2000876977635784 1106 64-65 1.177638778027628 1106 66-67 1.201998541414234 1106 68-69 0.868187170317924 1106 70-71 0.7549438921634142 1106 72-73 0.830781612848849 1106 74-75 1.039507023040386 1106 76-77 1.040785793036175 1106 78-79 0.9999327923203261 1106 80-81 1.1694673623305043 1106 82-83 1.0870695759795659 1106 84-85 1.1581313115406395 1106 86-87 1.162993924781425 1106 88-89 1.2548038173165956 1106 90-91 1.0664620463138057 1106 92-93 0.9010287720838441 1106 94-95 0.8599235594807695 1106 96-97 0.7295856942161301 1106 98-99 0.8682415680059634 1106 100-101 0.8197995931331263 1106 102-103 0.708834089138616 1106 104-105 0.697718409286388 1106 106-107 0.7751467885778887 1106 108-109 0.6158588280432191 1106 110-111 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116-117 0.12447588434132939 2313 118-119 0.03207549668001164 2313 120-121 0.37371426229448446 2314 1 -0.7066078387176518 2314 2 -5.107547388843052 2314 3 -1.8343103662802491 2314 4 -0.6193886127440891 2314 5 -3.2005184272161387 2314 6 -0.7985380720376156 2314 7 -0.30425374384392256 2314 8 -0.2828379007598443 2314 9 -0.08133842061664609 2314 10-11 0.038349087720213504 2314 12-13 0.05134361481778171 2314 14-15 0.025932415805094422 2314 16-17 -0.1403503708908289 2314 18-19 0.014841958189293791 2314 20-21 0.09605369845191802 2314 22-23 0.29795127608938543 2314 24-25 0.14262270577246738 2314 26-27 -0.037934248924351266 2314 28-29 0.0468753093985157 2314 30-31 0.06717289873282795 2314 32-33 0.0013641449331132094 2314 34-35 0.05530986813534611 2314 36-37 0.0071891475036380825 2314 38-39 -0.14731942888685978 2314 40-41 -0.07021557779177812 2314 42-43 -0.11946910961571433 2314 44-45 0.05315176470774219 2314 46-47 0.13296194322268917 2314 48-49 0.13301314736445136 2314 50-51 0.08692305456899163 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2314 116-117 0.46608137559054796 2314 118-119 0.2720113256934553 2314 120-121 0.13461051852539896 2315 1 -0.2658365364299726 2315 2 -0.11126466435209892 2315 3 -0.3029364098751266 2315 4 0.07309804381268492 2315 5 -0.003585935054907452 2315 6 -0.17905745443452048 2315 7 -0.020457460040077535 2315 8 -0.039993170408024525 2315 9 -0.29327979041756436 2315 10-11 -0.21527617334714932 2315 12-13 -0.4334664468669729 2315 14-15 -1.1152155790356275 2315 16-17 -0.40276206670889536 2315 18-19 -0.20549755081516707 2315 20-21 -0.24109261260546333 2315 22-23 -0.18027880858548428 2315 24-25 -0.26555281784636975 2315 26-27 -0.18311091125563195 2315 28-29 -0.2084348143487631 2315 30-31 -0.16263573281543842 2315 32-33 -0.1566624286217717 2315 34-35 -0.1667868198844218 2315 36-37 -0.3398491207899639 2315 38-39 -0.08730064822663053 2315 40-41 -0.11865133228322122 2315 42-43 -0.23154054088551135 2315 44-45 -0.24708407289352152 2315 46-47 -0.2406185788580828 2315 48-49 -0.0949913535179121 2315 50-51 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114-115 -0.07405019626982678 2315 116-117 -0.013825476652069568 2315 118-119 0.014319619945386108 2315 120-121 0.058990837688689624 2316 1 -1.4209979605296859 2316 2 -3.1530568731772064 2316 3 -1.4081864973211289 2316 4 -3.433841151391949 2316 5 -4.055759343517405 2316 6 -1.6270080529222497 2316 7 -7.643901347300883 2316 8 -3.513588779523186 2316 9 -2.105882645985986 2316 10-11 -1.0943127902947154 2316 12-13 -0.8439481586822808 2316 14-15 -4.640228503189306 2316 16-17 -3.5533132808489327 2316 18-19 -5.303042042772162 2316 20-21 -1.6155470185448095 2316 22-23 -3.673084335984285 2316 24-25 -1.3072812783341732 2316 26-27 -3.627621777689278 2316 28-29 -2.1756550216383914 2316 30-31 -1.0008359196374315 2316 32-33 -0.862280276208466 2316 34-35 -0.7449057610533529 2316 36-37 -0.6941971144824919 2316 38-39 -0.7823059193335737 2316 40-41 -0.6911525881782197 2316 42-43 -0.781545856628469 2316 44-45 -0.9993001696124466 2316 46-47 -0.839645229802052 2316 48-49 -0.7501632873689701 2316 50-51 -0.5753153452181579 2316 52-53 -0.7627012005437948 2316 54-55 -1.028377717316964 2316 56-57 -0.8470356653950688 2316 58-59 -0.8785142477786465 2316 60-61 -0.5545740326903328 2316 62-63 -0.8030596108450396 2316 64-65 -1.019547111843437 2316 66-67 -0.9038250140649247 2316 68-69 -0.7912803564796569 2316 70-71 -0.7929353268121027 2316 72-73 -0.6509275828774257 2316 74-75 -0.6821257691227487 2316 76-77 -0.7476294103526477 2316 78-79 -0.8801133434473947 2316 80-81 -0.8084483751675116 2316 82-83 -0.887378069068653 2316 84-85 -1.0984967742494334 2316 86-87 -1.0514383288159692 2316 88-89 -1.0024375728373904 2316 90-91 -1.1204501273051548 2316 92-93 -0.8821002743696695 2316 94-95 -0.9310249246889732 2316 96-97 -0.8217818741950857 2316 98-99 -0.5861714356671115 2316 100-101 -0.5639864163120301 2316 102-103 -0.6108460839216363 2316 104-105 -0.4783474988429717 2316 106-107 -0.4479505354356874 2316 108-109 -0.7144162309576672 2316 110-111 -0.7309449762148787 2316 112-113 -1.1501713998859735 2316 114-115 -0.7659217354574039 2316 116-117 -0.5199356594243554 2316 118-119 -0.5462137073282705 2316 120-121 -1.063286710641961 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49924.0 3 8322.0 4 7531.0 5 8396.0 6 9724.0 7 10486.0 8 12185.0 9 13377.0 10 15505.0 11 17468.0 12 18412.0 13 20144.0 14 24176.0 15 24057.0 16 19947.0 17 20462.0 18 21658.0 19 23038.0 20 25328.0 21 27990.0 22 31142.0 23 35469.0 24 40273.0 25 46596.0 26 53567.0 27 62024.0 28 72273.0 29 84852.0 30 100419.0 31 120259.0 32 147744.0 33 186121.0 34 240868.0 35 321857.0 36 448422.0 37 548699.0 38 233119.0 39 982.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.92403781282543 19.851479123149726 14.156007300200239 21.068475763824605 2 13.745236816572099 22.584154380334347 39.690215238854506 23.98039356423905 3 22.01913628632937 25.993546432504438 30.019444057059413 21.967873224106786 4 11.349702689034586 16.40148934301872 42.43803965129017 29.810768316656528 5 11.096303902885227 39.41098720490548 35.89273684001356 13.59997205219573 6 26.970929084507222 38.67029924886457 18.699976663255914 15.658795003372289 7 25.39841451389247 33.20935473336856 24.016265701757224 17.37596505098174 8 23.181219262413276 32.76862263568117 20.899508198612395 23.15064990329316 9 22.645333217130627 20.57851644818315 24.781679361918986 31.994470972767235 10-11 24.105771864543073 25.7835098280118 28.841926870173495 21.268791437271638 12-13 26.95398543064178 23.921040997943376 28.08958169155759 21.035391879857254 14-15 21.961707283163516 26.37708151938181 29.291865360429366 22.369345837025307 16-17 19.801471627291146 29.82990057239207 29.30139199932837 21.067235800988414 18-19 19.237801789741873 31.886556933102018 29.0467457548446 19.828895522311505 20-21 21.26924965449338 28.1048481181107 27.72333252992895 22.90256969746697 22-23 19.877883125952312 28.275168442177595 28.900459512279053 22.946488919591037 24-25 22.410248064255597 27.99988723041421 28.294818141422674 21.29504656390752 26-27 21.534014691894086 26.32446665874265 31.47749040227622 20.664028247087042 28-29 19.680287726944 30.671767226287066 29.28375457765985 20.36419046910908 30-31 20.675814664424376 27.92865254050686 28.566723941812043 22.828808853256717 32-33 21.223190579427992 28.087203423914524 29.39281920135367 21.296786795303817 34-35 22.94985896461664 26.93905624076363 29.172113806468236 20.93897098815149 36-37 21.936295054484493 26.550512149424865 30.979502803960003 20.53368999213064 38-39 20.983969126936678 28.69669054875386 30.22335795360676 20.095982370702703 40-41 20.5847137851263 29.49177097681738 29.110188539545707 20.813326698510608 42-43 19.839018186573266 29.600183199021572 27.434502183260555 23.126296431144603 44-45 21.136761825909083 27.35834093787008 27.796125146750473 23.708772089470365 46-47 22.768418049346337 28.87510619104825 28.21670967707829 20.139766082527125 48-49 22.162809027644276 28.195304189575403 28.68364476399195 20.95824201878837 50-51 19.098861961132542 28.84087316917845 31.074746146793753 20.985518722895254 52-53 20.718350202331862 30.294991205052046 27.5322721724002 21.454386420215883 54-55 19.74379963095073 28.329826932650032 28.617564880429093 23.308808555970153 56-57 21.970102631322433 26.988594097095746 28.457041570687615 22.58426170089421 58-59 24.112596878787365 26.927663090844806 27.630347826059083 21.329392204308746 60-61 21.719186722739206 27.956345736916923 30.52463105230251 19.799836488041365 62-63 20.242051749359298 28.60104604779484 29.71204127670571 21.44486092614016 64-65 19.67877012192094 31.261003080462054 28.16798209711705 20.892244700499955 66-67 21.01944836389221 28.1671273886738 27.138993229501075 23.674431017932914 68-69 19.993520582724567 28.844350105154344 28.09878451643583 23.06334479568526 70-71 22.507996504092443 28.109345786008937 27.60842695693293 21.77423075296569 72-73 21.573387393261147 26.591733540730374 30.539523312542904 21.295355753465582 74-75 19.879365104794555 30.8200369274148 28.372446220947694 20.928151746842946 76-77 20.69921896844484 27.98854771201803 27.965938608673273 23.34629471086386 78-79 21.29218603918332 28.36101653226318 28.697858245698704 21.648939182854797 80-81 22.74494632844751 27.67024113464257 28.132471201041128 21.45234133586879 82-83 21.41716009411309 27.114155671593643 30.30851119634212 21.16017303795115 84-85 20.75686091289431 28.896113162133453 29.341551817076102 21.005474107896138 86-87 20.964904294347935 29.498797187624625 28.157703064350464 21.378595453676976 88-89 20.13223056247757 29.026273807888536 26.995948517627404 23.84554711200649 90-91 21.090340342025186 26.824124186030257 28.031881997751295 24.05365347419326 92-93 22.37668104657896 28.334570880861914 28.485288303164474 20.80345976939465 94-95 21.400277093768754 27.529866910051904 29.164559883264836 21.90529611291451 96-97 18.642254453913992 28.385320039243073 31.327444521717446 21.644980985125493 98-99 20.166478755750177 29.717559549533355 28.05206642409027 22.063895270626197 100-101 19.147769176997222 27.81613747282417 29.111711099282367 23.924382250896244 102-103 21.015931497860834 26.813565798166405 29.04915553541097 23.121347168561794 104-105 23.05489514842856 27.043643808630442 28.05444728049624 21.84701376244476 106-107 20.662322162483687 27.524642145664597 30.755170075352602 21.057865616499114 108-109 19.842418974971824 27.71169572258099 30.10159394916553 22.344291353281655 110-111 19.306961155135248 29.914128895751567 28.47238914217991 22.30652080693327 112-113 20.725650065790603 27.358089487717884 27.23870330257443 24.67755714391708 114-115 19.62350478852293 27.662165540583857 28.141674893267865 24.572654777625356 116-117 21.921008008087767 27.18543073431332 27.364426750229136 23.52913450736978 118-119 21.49547153507707 25.993412704710746 29.593884989254963 22.917230770957225 120-121 20.219439755834728 29.689530343407096 27.630523736046626 22.460506164711553 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8621.0 1 10432.5 2 6897.0 3 1509.0 4 1454.0 5 1463.0 6 1538.0 7 1589.0 8 1669.5 9 1747.5 10 1703.5 11 1688.0 12 1778.0 13 1879.5 14 1993.5 15 2195.0 16 2408.0 17 2547.0 18 2825.0 19 3141.0 20 3390.5 21 3793.5 22 4422.5 23 5366.5 24 6572.5 25 8383.5 26 11201.5 27 15315.5 28 22092.0 29 32295.5 30 44429.5 31 57316.0 32 69859.5 33 80540.0 34 88824.0 35 95592.5 36 100169.5 37 104149.0 38 107261.5 39 106120.0 40 104291.5 41 104385.5 42 105669.0 43 108694.5 44 109887.0 45 111004.0 46 119415.0 47 163815.0 48 260542.5 49 256171.5 50 157794.0 51 105903.0 52 80221.5 53 65980.0 54 56483.5 55 48006.5 56 39420.5 57 31222.5 58 24653.0 59 19793.0 60 15769.0 61 12195.0 62 9157.5 63 6495.0 64 4454.5 65 3096.0 66 2086.0 67 1345.0 68 873.5 69 584.5 70 412.0 71 293.0 72 188.5 73 130.5 74 102.5 75 90.5 76 72.5 77 49.0 78 38.0 79 24.5 80 21.5 81 16.0 82 13.0 83 11.0 84 6.5 85 6.5 86 7.5 87 9.5 88 5.5 89 4.0 90 5.5 91 7.0 92 6.0 93 2.5 94 3.0 95 3.0 96 2.5 97 2.5 98 3.5 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19198709978634973 2 1.0150925394948516 3 1.0660628466742113 4 1.0312685548411324 5 1.0376120902710466 6 1.0553105541205068 7 1.027176974488838 8 1.0163612465808345 9 1.0096688167022752 10-11 0.9953324266306691 12-13 0.9894646563579986 14-15 0.9888937381693065 16-17 1.0166467056751805 18-19 1.054929941994712 20-21 1.039071103419927 22-23 1.0082890977462688 24-25 0.9961570862365581 26-27 0.9992019832429169 28-29 1.0351222526148052 30-31 1.0399909160572645 32-33 1.028001634094727 34-35 1.0364385362165125 36-37 1.0505211848709217 38-39 1.0325848384428398 40-41 1.043939766862386 42-43 1.0375486549167474 44-45 1.0109058061111083 46-47 1.0045781295197689 48-49 1.0065922020187668 50-51 0.9873712896661271 52-53 1.0056406717042796 54-55 1.0179629892768878 56-57 1.0809384372573596 58-59 1.0488084303048448 60-61 1.0519643391812272 62-63 1.0095895225094011 64-65 1.0000900782031048 66-67 1.0031666928866132 68-69 1.0201990855159324 70-71 0.9975843817082888 72-73 1.001913844639205 74-75 1.009526087155102 76-77 1.0273831393903101 78-79 1.1581868399551385 80-81 1.1237255837321303 82-83 1.1395368457911912 84-85 1.088455526741808 86-87 1.0612734774246262 88-89 1.0574832150052524 90-91 1.0685368254918777 92-93 1.0489194421748684 94-95 1.0445741204053773 96-97 1.0557070250848766 98-99 1.1025223165576425 100-101 1.085886394892693 102-103 1.0513458444768107 104-105 1.0300791419480235 106-107 1.0324738265728162 108-109 1.0293337765350086 110-111 1.0326641326357138 112-113 1.036835007180882 114-115 1.0421160004262857 116-117 1.0794001299156057 118-119 1.0762917975549477 120-121 1.04547807420414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 3152816.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.961387082709734 #Duplication Level Percentage of deduplicated Percentage of total 1 78.2014062544874 40.634535408016575 2 13.139571692424571 13.655007416221771 3 3.7468177589198155 5.840695436988106 4 1.6233453276656693 3.374050997589764 5 0.8683426786579077 2.2560145023090286 6 0.5003512893483709 1.559936821389816 7 0.35146230198566597 1.2783728102920189 8 0.235502757936531 0.9789639971348624 9 0.18370698138982192 0.8591102612813422 >10 1.0316780650128063 9.874548156156902 >50 0.07569452216408562 2.7054983132238934 >100 0.03662869202127754 3.4053842373473557 >500 0.0023714064031059036 0.8544767070416691 >1k 0.0018721629497733259 2.3549262606467383 >5k 6.240543165911087E-4 2.2351419277655706 >10k+ 6.240543165911087E-4 8.133336746594553 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 64688 2.051753099451411 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 45398 1.4399191072361979 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 31043 0.9846118517541145 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 26959 0.8550768582752688 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 17925 0.5685393629060497 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 16290 0.516680960766502 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 13419 0.4256195096700854 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12088 0.3834032813840072 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 11597 0.36782990190356807 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 11415 0.3620572846623463 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9460 0.30004922583493615 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8936 0.28342916300856125 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 8002 0.2538048525508625 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7040 0.22329244713297572 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6881 0.21824933646619402 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6536 0.20730673784959225 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6206 0.19683990439023402 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5393 0.17105343286763325 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5387 0.17086312680473584 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5088 0.16137954133701427 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4959 0.1572879609847197 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4426 0.14038243906399867 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4039 0.1281076980071149 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3808 0.12078091458556414 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3643 0.11554749785588503 No Hit ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3490 0.11069469325200075 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.171767714957041E-5 0.0 9 0.0 0.0 0.0 3.171767714957041E-5 0.0 10-11 0.0 1.5858838574785206E-5 0.0 6.343535429914083E-5 0.0 12-13 0.0 3.171767714957041E-5 0.0 6.343535429914083E-5 0.0 14-15 0.0 3.171767714957041E-5 0.0 1.1101187002349644E-4 0.0 16-17 0.0 3.171767714957041E-5 0.0 3.0131793292091896E-4 0.0 18-19 0.0 3.171767714957041E-5 0.0 3.80612125794845E-4 0.0 20-21 0.0 3.171767714957041E-5 0.0 6.343535429914084E-4 0.0 22-23 0.0 3.171767714957041E-5 0.0 0.0010784010230853942 0.0 24-25 0.0 3.171767714957041E-5 0.0 0.001443154310305454 0.0 26-27 0.0 3.171767714957041E-5 0.0 0.0018079075975255138 0.0 28-29 0.0 3.171767714957041E-5 0.0 0.0030290381677839746 0.0 30-31 0.0 3.171767714957041E-5 0.0 0.0049320987967581996 0.0 32-33 0.0 3.171767714957041E-5 0.0 0.008214878381738738 0.0 34-35 0.0 7.929419287392604E-5 0.0 0.011640387513892344 0.0 36-37 0.0 9.515303144871125E-5 0.0 0.014970743614597236 0.0 38-39 0.0 9.515303144871125E-5 0.0 0.018110793652404707 0.0 40-41 0.0 9.515303144871125E-5 0.0 0.0245494821137675 0.0 42-43 0.0 9.515303144871125E-5 0.0 0.03155908876382256 0.0 44-45 0.0 9.515303144871125E-5 0.0 0.04667256192559287 0.0 46-47 0.0 1.903060628974225E-4 0.0 0.07805720346509279 0.0 48-49 0.0 1.903060628974225E-4 0.0 0.0970243744005359 0.0 50-51 0.0 1.903060628974225E-4 0.0 0.12065404387696585 0.0 52-53 0.0 1.903060628974225E-4 0.0 0.1483594348671156 0.0 54-55 0.0 1.903060628974225E-4 0.0 0.17863395770638058 0.0 56-57 0.0 2.061649014722077E-4 0.0 0.20933666918716476 0.0 58-59 0.0 2.220237400469929E-4 0.0 0.28460271706309537 0.0 60-61 0.0 2.220237400469929E-4 0.0 0.36175596672942534 0.0 62-63 0.0 2.220237400469929E-4 0.0 0.44553821092001566 0.0 64-65 0.0 2.220237400469929E-4 0.0 0.4898319470593907 0.0 66-67 0.0 2.220237400469929E-4 0.0 0.6662139496881518 0.0 68-69 0.0 2.220237400469929E-4 0.0 0.8541887633150809 0.0 70-71 0.0 2.220237400469929E-4 0.0 0.9056348356516841 0.0 72-73 0.0 2.220237400469929E-4 0.0 1.073817818737281 0.0 74-75 0.0 2.220237400469929E-4 0.0 1.1525569522610897 0.0 76-77 0.0 2.220237400469929E-4 0.0 1.2040347422748425 0.0 78-79 0.0 2.220237400469929E-4 0.0 1.2706101466117907 0.0 80-81 0.0 2.3788257862177812E-4 0.0 1.3363291736657006 0.0 82-83 0.0 2.537414171965633E-4 0.0 1.4652456724401297 0.0 84-85 0.0 2.537414171965633E-4 0.0 1.7493091889916825 0.0 86-87 0.0 2.537414171965633E-4 0.0 2.334357602854084 0.0 88-89 0.0 2.537414171965633E-4 0.0 3.0744895991393095 0.0 90-91 0.0 2.537414171965633E-4 0.0 3.879626974742579 0.0 92-93 0.0 2.537414171965633E-4 0.0 4.7174335578099065 0.0 94-95 0.0 2.537414171965633E-4 0.0 5.456851906359267 0.0 96-97 0.0 2.537414171965633E-4 0.0 6.204929180770461 0.0 98-99 0.0 2.537414171965633E-4 0.0 6.981504788100542 0.0 100-101 0.0 2.537414171965633E-4 0.0 7.801549471964111 0.0 102-103 0.0 2.537414171965633E-4 0.0 8.713385113498536 0.0 104-105 0.0 2.854590943461337E-4 0.0 9.56990512608411 0.0 106-107 0.0 3.1717677149570414E-4 0.0 10.42014503859407 0.0 108-109 0.0 3.330356100704893E-4 0.0 11.237002095904106 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3880 0.0 52.05579 2 ACCTGGG 4165 0.0 46.228302 3 GTACCTG 4840 0.0 42.60052 1 TATAACG 615 0.0 41.169086 2 GTACCCG 465 0.0 35.918835 1 TAACGCA 725 0.0 34.085274 4 ATAACGC 800 0.0 32.329624 3 TACCCGG 525 0.0 31.785742 2 GTACCGG 395 0.0 30.619593 1 CCTGGGG 2810 0.0 29.655006 4 GTATAAC 1245 0.0 26.830936 1 TACCGGG 430 0.0 26.764257 2 AGTACGC 1095 0.0 26.275414 2 GTACACG 450 0.0 23.037598 1 GTACGCA 1200 0.0 22.510996 3 GAGTACG 1450 0.0 21.845999 1 TACGCAG 1355 0.0 19.934023 4 GTACATG 11835 0.0 19.708973 1 TACACGG 450 7.2759576E-12 19.181051 2 AGTACTT 6920 0.0 18.97819 12-13 >>END_MODULE