##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003787_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3152816 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79401018010566 31.0 31.0 34.0 30.0 34.0 2 31.989134792515642 31.0 31.0 34.0 30.0 34.0 3 32.04440633389326 33.0 31.0 34.0 30.0 34.0 4 35.776625086906435 37.0 35.0 37.0 35.0 37.0 5 35.63080655515577 37.0 35.0 37.0 33.0 37.0 6 35.69498124850927 37.0 35.0 37.0 33.0 37.0 7 35.64576270863888 37.0 35.0 37.0 33.0 37.0 8 35.647591867080095 37.0 35.0 37.0 33.0 37.0 9 37.25431106667817 39.0 37.0 39.0 33.0 39.0 10-11 37.165070527426906 39.0 37.0 39.0 33.0 39.0 12-13 37.13871710242526 39.0 37.0 39.0 33.0 39.0 14-15 38.29273719113326 40.0 38.0 41.0 33.0 41.0 16-17 38.320324592364415 40.0 38.0 41.0 33.0 41.0 18-19 38.33714574526392 40.0 38.0 41.0 33.5 41.0 20-21 38.2767879571786 40.0 38.0 41.0 33.5 41.0 22-23 38.197186261424704 40.0 37.5 41.0 33.0 41.0 24-25 38.08417411609177 40.0 37.0 41.0 33.0 41.0 26-27 37.9084654797489 40.0 37.0 41.0 33.0 41.0 28-29 37.77965697966516 40.0 37.0 41.0 33.0 41.0 30-31 37.61804637505011 40.0 37.0 41.0 32.0 41.0 32-33 37.38852140435725 39.0 36.0 41.0 31.5 41.0 34-35 37.35618729415228 39.0 36.0 41.0 31.0 41.0 36-37 37.23001675327707 39.0 36.0 41.0 31.0 41.0 38-39 37.030940752647794 39.0 36.0 41.0 31.0 41.0 40-41 37.09719866303647 39.0 36.0 41.0 31.0 41.0 42-43 36.99462861137472 39.0 36.0 41.0 31.0 41.0 44-45 36.79748675469803 39.0 36.0 41.0 30.0 41.0 46-47 36.68157149037559 39.0 36.0 41.0 30.0 41.0 48-49 36.483687757230356 39.0 36.0 41.0 30.0 41.0 50-51 36.286833738473796 39.0 35.0 41.0 30.0 41.0 52-53 36.152346822649974 39.0 35.0 41.0 29.0 41.0 54-55 35.99089496500906 39.0 35.0 40.5 28.5 41.0 56-57 35.715029992235515 38.5 35.0 40.0 28.0 41.0 58-59 35.46326997833049 38.0 34.0 40.0 28.0 41.0 60-61 35.09383056289996 38.0 34.0 40.0 27.0 41.0 62-63 34.89421250716819 38.0 34.0 40.0 26.5 41.0 64-65 34.48080144860975 37.0 33.5 40.0 26.0 41.0 66-67 34.11225520296776 37.0 33.0 39.0 26.0 41.0 68-69 33.79028842786893 36.0 33.0 39.0 26.0 41.0 70-71 33.34439672343708 36.0 32.0 39.0 25.0 40.0 72-73 32.93723103409777 35.0 32.0 38.0 24.5 40.0 74-75 32.57158806603367 35.0 32.0 37.0 24.0 39.5 76-77 31.542193074381757 34.0 30.5 36.0 22.5 39.0 78-79 31.977940672719246 35.0 31.5 36.0 24.0 39.0 80-81 31.73679783406326 35.0 31.0 36.0 23.0 38.5 82-83 31.411235701671142 35.0 31.0 36.0 22.0 37.0 84-85 31.06001983623529 34.0 31.0 35.0 20.0 37.0 86-87 30.822099513577704 34.0 31.0 35.0 20.0 36.5 88-89 30.501378291660536 34.0 31.0 35.0 19.0 36.0 90-91 30.190997191082513 34.0 31.0 35.0 18.0 36.0 92-93 29.951529680133568 34.0 30.5 35.0 14.0 36.0 94-95 29.612919212538884 34.0 30.0 35.0 7.0 35.0 96-97 29.275484519236137 34.0 30.0 35.0 2.0 35.0 98-99 28.963891010449075 34.0 30.0 35.0 2.0 35.0 100-101 28.06060915067673 33.5 28.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.09743039985484003 1101 2 0.14891627638558091 1101 3 0.15933477231677529 1101 4 0.1578901350564621 1101 5 0.221974650018673 1101 6 0.1502679606343662 1101 7 0.08298006064291741 1101 8 0.053790991288536816 1101 9 0.147059214289321 1101 10-11 0.2346307424809737 1101 12-13 0.24148430102894025 1101 14-15 0.3288490123306076 1101 16-17 0.2803793745752401 1101 18-19 0.22878487255751878 1101 20-21 0.2275590366819884 1101 22-23 0.21359218478495023 1101 24-25 0.24547328200258534 1101 26-27 0.3249226876803135 1101 28-29 0.23308476126025113 1101 30-31 0.25258014325432043 1101 32-33 0.33255452323768253 1101 34-35 0.38385212152471127 1101 36-37 0.4053970574503012 1101 38-39 0.3075007108662007 1101 40-41 0.38840756632207984 1101 42-43 0.35005453887021787 1101 44-45 0.4282700453680732 1101 46-47 0.3642332309221388 1101 48-49 0.3733759440237705 1101 50-51 0.3661979569156628 1101 52-53 0.36435776699515543 1101 54-55 0.3923164372257446 1101 56-57 0.25528303479082126 1101 58-59 0.16399294629942318 1101 60-61 0.17258789022059062 1101 62-63 0.0050849090235516314 1101 64-65 0.10217995910456779 1101 66-67 0.10336240779743378 1101 68-69 0.17322949542049315 1101 70-71 0.24467407063396962 1101 72-73 0.1386831216293487 1101 74-75 0.17123458255573354 1101 76-77 0.1494858146671163 1101 78-79 0.21317543477158551 1101 80-81 0.09377836948533513 1101 82-83 0.23765117853422524 1101 84-85 0.16932857579198668 1101 86-87 -0.014236936232187247 1101 88-89 -5.467267886274385E-4 1101 90-91 0.004738963594856926 1101 92-93 0.18476068473801988 1101 94-95 0.07023949743976843 1101 96-97 0.14073880642453318 1101 98-99 0.025226000345249133 1101 100-101 0.008413929586829738 1102 1 0.16314762872040944 1102 2 0.27287026125221914 1102 3 0.27503502621305387 1102 4 0.11182964827331432 1102 5 0.17080284426442915 1102 6 0.18357170174708415 1102 7 0.1322904813793997 1102 8 0.11800042605580785 1102 9 0.22197146488841923 1102 10-11 0.2508737809377095 1102 12-13 0.18742941486070208 1102 14-15 0.23690921562525347 1102 16-17 0.2602722954979342 1102 18-19 0.2596229505339167 1102 20-21 0.2594252045320573 1102 22-23 0.2513753757641908 1102 24-25 0.18924166834553802 1102 26-27 0.2549319940133188 1102 28-29 0.2363991136530359 1102 30-31 0.25332042243483954 1102 32-33 0.3273077360777492 1102 34-35 0.3402092800889207 1102 36-37 0.326612536301333 1102 38-39 0.2235697011201836 1102 40-41 0.3057927531835105 1102 42-43 0.47053123058547186 1102 44-45 0.5336849599183182 1102 46-47 0.3574272570118424 1102 48-49 0.38310804265456255 1102 50-51 0.38018267908804404 1102 52-53 0.3049987209943481 1102 54-55 0.2699396383793129 1102 56-57 0.3856390165343555 1102 58-59 0.2429852287572274 1102 60-61 0.24046994574194258 1102 62-63 0.09686886994524002 1102 64-65 -0.0677336811092033 1102 66-67 -0.05445051276527124 1102 68-69 0.08732685512891436 1102 70-71 0.12641709150589264 1102 72-73 0.09549191976719129 1102 74-75 0.068561004169446 1102 76-77 0.11793510770434779 1102 78-79 0.16086570807273404 1102 80-81 0.11082728972452927 1102 82-83 0.11119103217410853 1102 84-85 0.07582923708752887 1102 86-87 0.03534146047574538 1102 88-89 -0.003170297992966198 1102 90-91 -0.035959457809969564 1102 92-93 -0.1229368332692431 1102 94-95 0.00845120129919863 1102 96-97 0.16453011104831816 1102 98-99 0.15604972627226488 1102 100-101 0.3861966966358956 1103 1 -0.014530504123595023 1103 2 0.08541801906794788 1103 3 0.10938932381604616 1103 4 0.09644438765409546 1103 5 0.13475000241823665 1103 6 0.09571177883349691 1103 7 0.12900779059816614 1103 8 0.13923939836915622 1103 9 0.0975414370561083 1103 10-11 0.18261405393647578 1103 12-13 0.09847820905464033 1103 14-15 0.12257722880094235 1103 16-17 0.16322632289100625 1103 18-19 0.0755201331170241 1103 20-21 0.09505742511051807 1103 22-23 0.1105733563232647 1103 24-25 0.16730387111194744 1103 26-27 0.13810838597852637 1103 28-29 0.05614123728022946 1103 30-31 0.1253346739943595 1103 32-33 0.28333720848255695 1103 34-35 0.17666256169833616 1103 36-37 0.14790071372746638 1103 38-39 0.2172706580628443 1103 40-41 0.38085152031464276 1103 42-43 0.46661603066251445 1103 44-45 0.5399523386850049 1103 46-47 0.3861732351988536 1103 48-49 0.29804180633547617 1103 50-51 0.2613256261540755 1103 52-53 0.33625711128456715 1103 54-55 0.44720117669746173 1103 56-57 0.23877710904642413 1103 58-59 0.3622248221686206 1103 60-61 0.3380880861065165 1103 62-63 0.29797105013321357 1103 64-65 0.45832632881545976 1103 66-67 0.4115627702254159 1103 68-69 0.3244674788724282 1103 70-71 0.2646021469405113 1103 72-73 0.42808233714914223 1103 74-75 0.2379466177558882 1103 76-77 0.27127457315615544 1103 78-79 0.4331007015588497 1103 80-81 0.3569117014659362 1103 82-83 0.49049386574576204 1103 84-85 0.33057000870924824 1103 86-87 0.3712493993586463 1103 88-89 0.2438892544125686 1103 90-91 0.3928951229195725 1103 92-93 0.4850787847537852 1103 94-95 0.4524363542120362 1103 96-97 0.4792374929742671 1103 98-99 0.6988293180753438 1103 100-101 0.85311306909351 1104 1 0.10068112344759328 1104 2 0.10381192487469448 1104 3 0.16230045992978148 1104 4 0.09362949926262587 1104 5 0.04433294600584503 1104 6 0.02557359086689104 1104 7 0.06351062850509948 1104 8 0.04525005060528997 1104 9 0.026075381613047455 1104 10-11 0.10701878636222517 1104 12-13 0.12122285137326827 1104 14-15 0.09376074910760224 1104 16-17 0.18663635213850682 1104 18-19 0.05295644209650874 1104 20-21 0.014406158544439052 1104 22-23 0.0488324595163121 1104 24-25 0.108001776959604 1104 26-27 0.052726518868041694 1104 28-29 0.1855419926138424 1104 30-31 0.08582666389131788 1104 32-33 0.20219929743596055 1104 34-35 0.056149055279540505 1104 36-37 0.13115966695123404 1104 38-39 0.12017014540044357 1104 40-41 0.263803350416417 1104 42-43 0.14505208829815075 1104 44-45 0.27237483537812324 1104 46-47 0.2511391572971391 1104 48-49 0.22042032599226502 1104 50-51 0.2635417418754784 1104 52-53 0.1957532819528609 1104 54-55 0.1729743380567328 1104 56-57 0.171128120385724 1104 58-59 0.2513803230997098 1104 60-61 0.13467493656808216 1104 62-63 0.06796863899931793 1104 64-65 0.013704168844050457 1104 66-67 0.1690686161207111 1104 68-69 0.07797309783747153 1104 70-71 0.02337802332490213 1104 72-73 0.03783816392044059 1104 74-75 0.06690991620567388 1104 76-77 0.0689155099781722 1104 78-79 0.20398008650347776 1104 80-81 0.26985750257040664 1104 82-83 0.3381390923865375 1104 84-85 0.35726302811620414 1104 86-87 0.2993683126696638 1104 88-89 0.2947215722562859 1104 90-91 0.1514567408764549 1104 92-93 0.20237513636174498 1104 94-95 0.2112600115045744 1104 96-97 0.21938148905321242 1104 98-99 0.3502809625174983 1104 100-101 0.5330277772660921 1105 1 -0.054797210969848464 1105 2 0.006704681178241856 1105 3 0.013175933664697936 1105 4 9.258773815119525E-4 1105 5 -0.05223871169529559 1105 6 0.04862330039424734 1105 7 0.03325681599000774 1105 8 0.09669568336517642 1105 9 0.049550402982774244 1105 10-11 -0.03533588249852926 1105 12-13 0.024470874604944015 1105 14-15 0.14071424476536265 1105 16-17 0.06603167050744219 1105 18-19 0.003693108515442134 1105 20-21 -0.11698900241527355 1105 22-23 -0.054628537524259 1105 24-25 0.03780301602015612 1105 26-27 0.016992924998319836 1105 28-29 0.042684293844679644 1105 30-31 0.1165482646247824 1105 32-33 0.1508297384952968 1105 34-35 0.09032039544373305 1105 36-37 0.14148646867852932 1105 38-39 0.2023932832854456 1105 40-41 0.320091454466116 1105 42-43 0.3036480790982594 1105 44-45 0.47611334839584885 1105 46-47 0.41437110198730664 1105 48-49 0.33417441770045997 1105 50-51 0.37771160879453447 1105 52-53 0.4160922752221836 1105 54-55 0.299776350351749 1105 56-57 0.2744492468307129 1105 58-59 0.28984199647381104 1105 60-61 0.22731580302914978 1105 62-63 0.27664635982769425 1105 64-65 0.08546918448960383 1105 66-67 0.11224848861046866 1105 68-69 0.09817464303485934 1105 70-71 0.13565766410740565 1105 72-73 0.2276520439504921 1105 74-75 0.27956024695139803 1105 76-77 0.2936494653629609 1105 78-79 0.24421349062711073 1105 80-81 0.25701149549225377 1105 82-83 0.23547095115932493 1105 84-85 0.15716652178990742 1105 86-87 0.290358607448038 1105 88-89 0.3030285811981379 1105 90-91 0.4155396052157876 1105 92-93 0.40906129064164176 1105 94-95 0.4231764052810796 1105 96-97 0.3077884199700698 1105 98-99 0.3172051840049974 1105 100-101 0.27800504171732143 1106 1 -0.036849790672107474 1106 2 0.07262326995192225 1106 3 -0.012567905221224862 1106 4 -0.057769573198157786 1106 5 -0.037327902892748455 1106 6 0.04814957038940548 1106 7 -4.84489987741199E-4 1106 8 0.04115944274928296 1106 9 0.05166563234200083 1106 10-11 0.03551953993002144 1106 12-13 -0.092929819282908 1106 14-15 0.04730400442800686 1106 16-17 -0.029559805425549257 1106 18-19 -0.053175538316672544 1106 20-21 -0.04186530294101232 1106 22-23 -0.05901941303855551 1106 24-25 -0.08207983697713672 1106 26-27 0.08641985344355874 1106 28-29 -0.03869398345963759 1106 30-31 -0.03183976028575586 1106 32-33 0.02512573058580614 1106 34-35 0.08587666083684553 1106 36-37 0.16384207760174974 1106 38-39 0.21970931605959265 1106 40-41 0.19470422238786256 1106 42-43 0.23124508094351626 1106 44-45 0.42602965573430396 1106 46-47 0.2917294047224246 1106 48-49 0.27354190049636173 1106 50-51 0.31174513844772633 1106 52-53 0.3246439350031096 1106 54-55 0.33676548778116455 1106 56-57 0.31122516247518206 1106 58-59 0.34182266508668135 1106 60-61 0.19237540179131685 1106 62-63 0.1444126579306868 1106 64-65 0.2081940387916532 1106 66-67 0.06537524190112265 1106 68-69 0.015149131408072947 1106 70-71 0.2276314031475195 1106 72-73 0.30152660245263263 1106 74-75 0.19024428689230888 1106 76-77 0.12547455619457537 1106 78-79 0.07764994517894763 1106 80-81 0.17363588029519406 1106 82-83 0.26624836070886815 1106 84-85 0.2600987027625621 1106 86-87 0.27794267207096013 1106 88-89 0.17622097065743958 1106 90-91 0.19407759088507603 1106 92-93 0.36767094393468724 1106 94-95 0.343199624327319 1106 96-97 0.46111338674242575 1106 98-99 0.4522194256620544 1106 100-101 0.3831132102487729 1107 1 -0.058685444206137305 1107 2 -0.04105505536892551 1107 3 0.005714717878994691 1107 4 -0.009368351642805806 1107 5 -0.0647179504433808 1107 6 0.01615332823432425 1107 7 0.016308272921271794 1107 8 0.0054071672518531955 1107 9 0.017286850014187394 1107 10-11 -0.07036938796099435 1107 12-13 -0.1620632856497437 1107 14-15 -0.039075598010249735 1107 16-17 -0.033904600484262915 1107 18-19 -0.06429141280312223 1107 20-21 -0.0931977586197732 1107 22-23 -0.06281434195130231 1107 24-25 0.0084355900717199 1107 26-27 0.00571815177833912 1107 28-29 -0.1752246818684995 1107 30-31 -0.10243868386949373 1107 32-33 -0.06684428423287869 1107 34-35 -0.020344051597867008 1107 36-37 0.013882017491575027 1107 38-39 0.07430163613454965 1107 40-41 0.009872951372265959 1107 42-43 0.010087265310225746 1107 44-45 0.0253210423795025 1107 46-47 0.021795531454635864 1107 48-49 0.09728102865162214 1107 50-51 -0.019391394381948146 1107 52-53 0.02668772139843867 1107 54-55 0.15803107078527745 1107 56-57 0.12734555417594606 1107 58-59 -0.0033959823662144117 1107 60-61 -0.1263343692661678 1107 62-63 0.03822513993482346 1107 64-65 0.08236292570273207 1107 66-67 -0.04909308975170745 1107 68-69 -0.0017221685841875 1107 70-71 -0.016201890650854978 1107 72-73 0.1416470529762961 1107 74-75 0.17576671620946627 1107 76-77 0.027477646101310427 1107 78-79 0.058007468999555556 1107 80-81 0.21001448103930542 1107 82-83 0.1453345002244042 1107 84-85 0.2364424168441026 1107 86-87 0.20109376313966365 1107 88-89 -0.01411631467897223 1107 90-91 0.10168633171078412 1107 92-93 0.04421308936320045 1107 94-95 0.16279386999941536 1107 96-97 0.14952987028022235 1107 98-99 0.07751511191951366 1107 100-101 0.06718745624482025 1108 1 0.034468449163551185 1108 2 0.06501766422762145 1108 3 -0.02363829168633913 1108 4 -0.037955321213573257 1108 5 0.013098733941575347 1108 6 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-1.1039302475949384 2316 50-51 -1.035169587551465 2316 52-53 -1.0233856004540485 2316 54-55 -1.1248823466409306 2316 56-57 -1.1306402300463674 2316 58-59 -0.9696431554939124 2316 60-61 -1.1688026056042204 2316 62-63 -1.1329593531269353 2316 64-65 -0.9663005060020353 2316 66-67 -1.0379376530980409 2316 68-69 -0.7242700660795904 2316 70-71 -0.9333427128617231 2316 72-73 -1.2730444751630685 2316 74-75 -1.0253766659307075 2316 76-77 -0.7821333792963792 2316 78-79 -0.7595279711566114 2316 80-81 -0.7284263676861968 2316 82-83 -0.941012051719504 2316 84-85 -0.9616522551517157 2316 86-87 -1.0476535054936775 2316 88-89 -0.9132972545527878 2316 90-91 -0.834855751477086 2316 92-93 -0.9312756252746546 2316 94-95 -1.0493678367411654 2316 96-97 -1.321575986654981 2316 98-99 -1.2931523835973984 2316 100-101 -1.4273099060975163 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 12.0 5 46.0 6 113.0 7 303.0 8 774.0 9 1524.0 10 2718.0 11 4274.0 12 6487.0 13 9128.0 14 10613.0 15 13242.0 16 16453.0 17 20680.0 18 18979.0 19 14800.0 20 13955.0 21 14302.0 22 15227.0 23 16932.0 24 20178.0 25 24829.0 26 31193.0 27 39089.0 28 50511.0 29 63882.0 30 79939.0 31 99497.0 32 124717.0 33 157956.0 34 207389.0 35 280439.0 36 411396.0 37 605772.0 38 623922.0 39 151468.0 40 71.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93878282194728 20.599757489749166 14.807215140103459 20.6542445482001 2 14.503629626481207 23.310239651139703 38.83570286227593 23.350427860103164 3 22.398814262736252 26.224346389622323 30.016846176228963 21.359993171412462 4 12.20391548380876 16.990081248001786 41.18280292919092 29.623200338998533 5 11.750701595018548 39.49434410381069 35.30954549837352 13.445408802797246 6 27.677860046383927 39.30486270051915 17.86111209788329 15.156165155213625 7 26.12055381601717 33.08972042770653 23.325084622762635 17.46464113351366 8 24.03406986008698 32.75411568578693 20.536212706355208 22.675601747770884 9 22.928328199298658 20.555116441936352 24.188281206388194 32.32827415237679 10-11 24.48212962634039 26.00367417572101 28.79367206966724 20.72052412827136 12-13 27.3718954737606 24.015626030824507 27.872289407310795 20.740189088104096 14-15 21.943890302196117 26.71314007790925 29.177829467764937 22.165140152129688 16-17 20.108039923674582 29.753417262536097 29.13799917280298 21.000543640986344 18-19 19.607947308057305 31.699439485209414 29.14857701813236 19.54403618860092 20-21 21.67450916452497 27.79952431132041 28.10725009690777 22.41871642724685 22-23 20.248739539510076 28.286918741848556 28.954813728425638 22.50952799021573 24-25 22.67595064221953 28.08877841269519 28.565463382576084 20.6698075625092 26-27 21.979008763741223 26.229577095325237 31.438323771533085 20.353090369400462 28-29 19.9836634091293 30.697025233688592 29.44730775361566 19.87200360356645 30-31 21.256027384093894 27.763112107996 28.609317024939358 22.371543482970747 32-33 21.40703954328976 28.16557966538134 29.50775914802656 20.91962164330234 34-35 23.188846191055447 26.970908554562918 29.512663176475627 20.327582077906012 36-37 22.309251538888226 26.60153109206935 31.014726407269606 20.074490961772824 38-39 21.01459668718665 28.6258329631275 30.59862283429587 19.760947515389983 40-41 20.964706311501473 29.17532812020488 29.059329271629053 20.800636296664596 42-43 20.14842055726863 29.57665342223298 27.648023636643078 22.626902383855317 44-45 21.655396215761396 27.068670314131932 28.09527925790286 23.18065421220381 46-47 23.12801907466799 28.78425318532301 28.263296774821793 19.824430965187208 48-49 22.49766118031931 28.20915478807415 28.750076718360436 20.5431073132461 50-51 19.522351985891635 28.695468069044704 30.981241713557097 20.800938231506564 52-53 21.23831646383248 30.26013070050389 27.411359591953833 21.0901932437098 54-55 20.298738624954186 28.286458705853974 28.499508133987376 22.91529453520446 56-57 22.381689283268962 27.02491931440759 28.649543086954875 21.943848315368573 58-59 24.566181541122116 26.990873524148114 27.59905677434335 20.843888160386427 60-61 21.8535783400542 28.216093846992706 30.562792038751248 19.367535774201848 62-63 20.629272974082742 28.436804589749286 29.724348912426144 21.209573523741824 64-65 20.062618164885933 31.074280195021558 28.205725175514484 20.65737646457803 66-67 21.519124297742447 27.862156121255428 27.334839288062785 23.28388029293934 68-69 20.53695288872532 28.82882911465195 27.973248327822198 22.66096966880053 70-71 22.796606513266298 28.328660516186094 27.658525242412303 21.21620772813531 72-73 22.028761644374384 26.635271392821007 30.4062883586388 20.929678604165808 74-75 20.299282844617498 30.90162453828791 28.344947599810773 20.454145017283825 76-77 21.246726504776966 27.904317596080546 27.831112465821878 23.01784343332061 78-79 21.459566700552053 28.4435665609929 28.651192517092035 21.445674221363017 80-81 22.990420499534693 27.72077859053646 28.25246909546958 21.036331814459267 82-83 21.830948054833573 27.120000418678586 30.14213027786778 20.906921248620066 84-85 20.72880858117422 28.84824480883746 29.430575476360094 20.99237113362823 86-87 21.14913174847898 29.171360586112005 27.83348450621976 21.84602315918925 88-89 20.36993689434942 29.204363221610702 27.083999406243574 23.3417004777963 90-91 21.581631787292192 26.483267463198125 28.054746352799082 23.8803543967106 92-93 22.512697431460197 28.267703373791512 28.31450299939451 20.905096195353785 94-95 21.506139651802247 27.73022802155321 29.11121948511774 21.652412841526804 96-97 18.79449024382739 28.180958262873762 31.27304309540599 21.751508397892856 98-99 20.468632729206014 29.714131275405038 27.826283630448422 21.990952364940526 100-101 19.44020823588732 27.789422394747596 28.725839931112507 24.044529438252574 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 167.0 1 133.5 2 298.5 3 450.0 4 308.0 5 237.5 6 374.5 7 660.5 8 1000.0 9 1227.0 10 1282.5 11 1346.5 12 1481.0 13 1654.5 14 1885.0 15 2135.5 16 2386.5 17 2642.0 18 2869.0 19 3083.0 20 3412.0 21 3944.5 22 4512.5 23 5307.5 24 6570.5 25 8205.0 26 10707.0 27 14950.0 28 21639.5 29 31723.0 30 43701.5 31 56365.5 32 68984.5 33 80286.0 34 88750.0 35 95147.0 36 100041.0 37 103768.5 38 106596.5 39 105543.5 40 104027.5 41 104169.0 42 105189.5 43 108272.5 44 108938.5 45 109306.0 46 116135.0 47 167352.0 48 278735.5 49 271507.0 50 160864.0 51 106226.5 52 80601.5 53 66406.0 54 57063.0 55 48705.5 56 40008.0 57 31849.0 58 25352.5 59 20278.0 60 16182.5 61 12337.5 62 9133.0 63 6499.0 64 4329.5 65 2840.5 66 1814.0 67 1153.0 68 699.0 69 411.0 70 265.0 71 157.5 72 92.5 73 60.5 74 45.0 75 33.0 76 26.0 77 14.5 78 11.0 79 12.5 80 9.0 81 5.5 82 2.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051033742533658805 2 0.004757651572435562 3 0.043104323246266195 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001443154310305454 16-17 0.0 18-19 0.0 20-21 0.01059370416795652 22-23 0.0 24-25 0.0 26-27 0.00529685208397826 28-29 0.012163729186860255 30-31 0.010435115782208666 32-33 0.0057884760797966015 34-35 0.005423722792576541 36-37 0.019712536348458015 38-39 0.04491223084379171 40-41 0.006295958914189727 42-43 0.0134007185956935 44-45 0.012639494344103809 46-47 0.004852804603884274 48-49 0.0016017426960533059 50-51 0.0024105434633673517 52-53 0.0011259775388097499 54-55 8.405184444636159E-4 56-57 0.00956287966059548 58-59 0.007295065744401196 60-61 0.006184947044166231 62-63 0.007152336197228129 64-65 0.07271277486539018 66-67 0.03506389208885009 68-69 0.02738821421865405 70-71 0.017190981015067167 72-73 1.903060628974225E-4 74-75 3.9647096436963016E-4 76-77 9.991068302114682E-4 78-79 9.83247991636683E-4 80-81 0.0013321424402819575 82-83 0.0012528482474080315 84-85 0.0017127545660768025 86-87 0.007754972063069967 88-89 2.537414171965633E-4 90-91 6.819300587157639E-4 92-93 4.123298029444154E-4 94-95 0.004868663442459059 96-97 0.009483585467721553 98-99 0.012369894088332462 100-101 0.013622742335740493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3152816.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.641900896586456 #Duplication Level Percentage of deduplicated Percentage of total 1 75.9484640193156 36.94277660075512 2 14.342479536783607 13.95290936479095 3 4.206102752690753 6.1377849977172945 4 1.7902374603906854 3.4832221251872144 5 0.932996299075203 2.269135675824898 6 0.5776879008233515 1.685990257260392 7 0.3909556864408024 1.3311779428367312 8 0.27940929746300286 1.0872799485424187 9 0.20595043719327114 0.9016038680007374 >10 1.185292398790192 10.810629097160495 >50 0.08831024541209685 2.97665997281062 >100 0.046404935105598566 4.157042975530472 >500 0.002493599535669426 0.8768237600779376 >1k 0.0018373891314914868 2.076862569058433 >5k 5.905893636936922E-4 1.879122372714459 >10k+ 7.874524849249229E-4 9.43097847173196 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 78596 2.4928825532476364 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 35626 1.1299739661305956 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 28654 0.9088383210437907 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 26165 0.82989302261851 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 21048 0.6675936686441581 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 18482 0.5862061090783605 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 17630 0.5591826481469264 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 16634 0.5275918417059543 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 15129 0.47985673759585085 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 13292 0.42159136467208996 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12383 0.39275999614313045 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11824 0.3750298146165206 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8547 0.27109098659737835 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7198 0.22830384012260788 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 6983 0.22148453953545022 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6858 0.21751982989175392 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6455 0.20473760600047702 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 6328 0.20070946100248158 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5668 0.17977579408376512 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5544 0.1758428021172184 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5290 0.1677865121212275 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4795 0.15208626193219016 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4384 0.1390502966237167 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3961 0.1256337191894484 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3351 0.10628593612821047 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3289 0.1043194401449371 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3205 0.10165515526437319 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.171767714957041E-5 0.0 9 0.0 0.0 0.0 3.171767714957041E-5 0.0 10-11 0.0 0.0 0.0 1.2687070859828165E-4 0.0 12-13 0.0 0.0 0.0 1.5858838574785207E-4 0.0 14-15 0.0 0.0 0.0 2.061649014722077E-4 0.0 16-17 0.0 0.0 0.0 3.0131793292091896E-4 0.0 18-19 0.0 0.0 0.0 3.80612125794845E-4 0.0 20-21 0.0 0.0 0.0 6.026358658418379E-4 0.0 22-23 0.0 0.0 0.0 0.0010942598616601794 0.0 24-25 0.0 0.0 0.0 0.0014748719874550244 0.0 26-27 0.0 0.0 0.0 0.0018079075975255138 0.0 28-29 0.0 0.0 0.0 0.0031559088763822565 0.0 30-31 0.0 0.0 0.0 0.005233416729679119 0.0 32-33 0.0 0.0 0.0 0.008547913991809227 0.0 34-35 0.0 0.0 0.0 0.012020999639687188 0.0 36-37 0.0 0.0 0.0 0.015541661803289503 0.0 38-39 0.0 0.0 0.0 0.018713429518246547 0.0 40-41 0.0 0.0 0.0 0.025627883136852894 0.0 42-43 0.0 0.0 0.0 0.033176690298450655 0.0 44-45 0.0 0.0 0.0 0.04886108164891323 0.0 46-47 0.0 0.0 0.0 0.08192676007734039 0.0 48-49 0.0 0.0 0.0 0.10203576739016804 0.0 50-51 0.0 0.0 0.0 0.12709273233832866 0.0 52-53 0.0 0.0 0.0 0.1558923831901386 0.0 54-55 0.0 0.0 0.0 0.18722944821391416 0.0 56-57 0.0 0.0 0.0 0.21910571374923243 0.0 58-59 0.0 3.171767714957041E-5 0.0 0.2977338354030175 0.0 60-61 0.0 3.171767714957041E-5 0.0 0.37804299394572977 0.0 62-63 0.0 3.171767714957041E-5 0.0 0.46475912327265534 0.0 64-65 0.0 3.171767714957041E-5 0.0 0.5100836839193914 0.0 66-67 0.0 3.171767714957041E-5 0.0 0.692777504300917 0.0 68-69 0.0 3.171767714957041E-5 0.0 0.8887293137309631 0.0 70-71 0.0 3.171767714957041E-5 0.0 0.9436326128768695 0.0 72-73 0.0 3.171767714957041E-5 0.0 1.121378475623062 0.0 74-75 0.0 3.171767714957041E-5 0.0 1.2047008134949837 0.0 76-77 0.0 3.171767714957041E-5 0.0 1.2597944187037875 0.0 78-79 0.0 9.515303144871125E-5 0.0 1.3306517094559278 0.0 80-81 0.0 9.515303144871125E-5 0.0 1.400113422413487 0.0 82-83 0.0 9.515303144871125E-5 0.0 1.5354051742949795 0.0 84-85 0.0 9.515303144871125E-5 0.0 1.8299989596601893 0.0 86-87 0.0 9.515303144871125E-5 0.0 2.436710547015747 0.0 88-89 0.0 9.515303144871125E-5 0.0 3.202740026693597 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3735 0.0 46.934246 2 ACCTGGG 3835 0.0 43.851562 3 GTACCTG 4305 0.0 41.61994 1 TATAACG 440 0.0 36.709656 2 TAACGCA 535 0.0 31.952368 4 ATAACGC 610 0.0 29.593817 3 CCTGGGG 2575 0.0 28.951878 4 GTACCGG 285 0.0 26.681387 1 GTACCCG 360 0.0 25.083284 1 AGTACGC 1285 0.0 23.660883 2 TACGCAG 1285 0.0 23.650002 4 GTACGCA 1300 0.0 23.022072 3 GAGTACG 1495 0.0 22.888882 1 GTACACG 405 0.0 22.296253 1 TACCGGG 280 6.548362E-11 22.056643 2 GTATAAC 1010 0.0 21.175047 1 TACACGG 495 0.0 21.114052 2 ACGCAGT 1590 0.0 19.710657 5 CCTGGGA 2295 0.0 19.449099 4 CTGGGGG 1735 0.0 19.431807 5 >>END_MODULE