##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003786_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1648421 Sequences flagged as poor quality 0 Sequence length 121 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.80025612389068 31.0 31.0 34.0 28.0 34.0 2 30.484547333478524 31.0 31.0 34.0 26.0 34.0 3 30.656536770642937 31.0 31.0 34.0 26.0 34.0 4 34.01778793160243 37.0 35.0 37.0 30.0 37.0 5 33.96682340251671 37.0 35.0 37.0 28.0 37.0 6 34.10384665082525 37.0 35.0 37.0 30.0 37.0 7 34.078257314120606 37.0 35.0 37.0 30.0 37.0 8 34.14980699712027 37.0 35.0 37.0 30.0 37.0 9 35.589710395584625 39.0 35.0 39.0 30.0 39.0 10-11 35.48936163759137 39.0 35.0 39.0 30.0 39.0 12-13 35.51970977074424 39.0 35.0 39.0 30.0 39.0 14-15 36.404430057612714 39.0 36.0 41.0 29.5 41.0 16-17 36.41174372323575 39.0 36.0 41.0 29.5 41.0 18-19 36.35099134262424 39.0 36.0 41.0 29.0 41.0 20-21 36.265074880749516 39.0 36.0 41.0 28.0 41.0 22-23 36.07512219269228 39.0 36.0 41.0 27.0 41.0 24-25 35.95491837340097 39.0 35.5 41.0 27.0 41.0 26-27 35.80734260240557 39.0 35.5 40.5 27.0 41.0 28-29 35.65281502722909 39.0 35.0 40.0 27.0 41.0 30-31 35.38234710671606 38.0 35.0 40.0 26.0 41.0 32-33 35.10315022679279 38.0 34.0 40.0 25.0 41.0 34-35 34.9801015638602 38.0 34.0 40.0 25.0 41.0 36-37 34.93407297043656 38.0 34.5 40.0 25.0 41.0 38-39 34.85596549667834 38.0 34.5 40.0 24.5 41.0 40-41 35.03840432753526 39.0 35.0 40.0 24.5 41.0 42-43 34.89822078219096 38.5 34.5 40.0 24.0 41.0 44-45 34.670523185521176 38.0 34.0 40.0 23.5 41.0 46-47 34.397113358783955 38.0 34.0 40.0 22.5 41.0 48-49 34.07526232679637 38.0 33.0 40.0 19.0 41.0 50-51 33.18929690898138 37.5 32.0 39.5 15.5 40.5 52-53 33.33749600375147 38.0 33.0 39.5 16.5 40.5 54-55 33.712504269237044 38.0 33.0 40.0 17.5 41.0 56-57 33.32165296365431 38.0 32.5 40.0 13.5 41.0 58-59 33.07049200416641 38.0 32.0 40.0 12.5 41.0 60-61 32.509433573098136 37.0 31.0 40.0 8.0 41.0 62-63 32.43077587582299 37.0 31.0 40.0 8.0 41.0 64-65 32.1027962516857 36.5 31.0 39.5 7.5 41.0 66-67 31.579050800735978 36.0 30.5 39.0 4.5 41.0 68-69 31.209287857895525 35.5 30.0 39.0 2.0 40.0 70-71 30.689273249976797 35.0 29.5 37.5 2.0 40.0 72-73 30.232042360537754 35.0 29.0 37.0 2.0 39.5 74-75 29.82498130028676 34.5 29.0 37.0 2.0 39.0 76-77 29.41313748126237 34.0 29.0 36.0 2.0 39.0 78-79 28.814352340815848 34.0 28.0 36.0 2.0 38.0 80-81 28.25582966972636 34.0 26.0 35.5 2.0 37.0 82-83 27.865593801583454 34.0 26.0 35.0 2.0 37.0 84-85 27.613033624298644 33.0 26.0 35.0 2.0 36.0 86-87 27.557919669793094 34.0 26.0 35.0 2.0 36.0 88-89 27.14507701612634 33.5 25.5 35.0 2.0 36.0 90-91 26.797977883077202 33.0 25.0 35.0 2.0 35.0 92-93 26.57527536958095 33.0 25.0 35.0 2.0 35.0 94-95 26.22048615007938 33.0 24.0 35.0 2.0 35.0 96-97 25.947285311216007 33.0 23.0 35.0 2.0 35.0 98-99 25.72292848732211 32.0 20.0 35.0 2.0 35.0 100-101 25.380481381879992 32.0 19.0 35.0 2.0 35.0 102-103 24.96857113565042 32.0 18.5 35.0 2.0 35.0 104-105 24.547787852739077 32.0 16.5 34.5 2.0 35.0 106-107 24.018126134039786 31.0 8.5 34.0 2.0 35.0 108-109 23.828830438340688 31.0 7.0 34.0 2.0 35.0 110-111 23.56494912404052 31.0 2.0 34.0 2.0 35.0 112-113 22.932954930809544 31.0 2.0 34.0 2.0 35.0 114-115 22.4224278870507 31.0 2.0 34.0 2.0 35.0 116-117 21.785237812427773 30.0 2.0 34.0 2.0 35.0 118-119 21.044841700026872 29.5 2.0 34.0 2.0 35.0 120-121 19.525664256885833 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6616865494276745 1101 2 0.8217518469252596 1101 3 0.8549300141176275 1101 4 1.110114677053737 1101 5 1.07601013472528 1101 6 1.0097771775444002 1101 7 0.9680070362804614 1101 8 1.1263956702230615 1101 9 1.0507436310423444 1101 10-11 1.1150649714442054 1101 12-13 0.7899763364729182 1101 14-15 1.091846762624563 1101 16-17 1.2828089598333534 1101 18-19 1.3493429818063731 1101 20-21 1.234470776284887 1101 22-23 1.2956666266643424 1101 24-25 1.2683134971098369 1101 26-27 1.1644396709797604 1101 28-29 1.2476117874289159 1101 30-31 1.2684767493156741 1101 32-33 1.343974601990034 1101 34-35 1.4464244306806577 1101 36-37 1.1305887797580425 1101 38-39 1.2869288350803103 1101 40-41 1.4459865293274348 1101 42-43 1.2497238927922325 1101 44-45 1.3683219853805966 1101 46-47 1.1638369351449995 1101 48-49 1.334503961985959 1101 50-51 0.9011042589478677 1101 52-53 1.0118729512845164 1101 54-55 1.0346189068225797 1101 56-57 1.2090164422941854 1101 58-59 0.8729478432760018 1101 60-61 0.998917058227903 1101 62-63 1.184877804098683 1101 64-65 1.1734296522239447 1101 66-67 1.1437872530305775 1101 68-69 1.2256526266562382 1101 70-71 0.8606104048140217 1101 72-73 0.7312208192404448 1101 74-75 0.9182179380819591 1101 76-77 0.8611314850086487 1101 78-79 1.094510429805588 1101 80-81 1.2118903147497342 1101 82-83 1.1751939975482095 1101 84-85 1.0911248406687442 1101 86-87 1.13127028478279 1101 88-89 1.2555733134224099 1101 90-91 1.3160153323975727 1101 92-93 1.404006991986538 1101 94-95 1.3979506817450016 1101 96-97 1.2752902594881235 1101 98-99 1.2997579132046155 1101 100-101 1.202402746150078 1101 102-103 1.1342274864962434 1101 104-105 1.2082626470960243 1101 106-107 1.2197778958870025 1101 108-109 1.2809069671157012 1101 110-111 1.1841635837180604 1101 112-113 1.113517233634557 1101 114-115 0.9805839317398437 1101 116-117 0.9185319774733998 1101 118-119 0.9174551869535463 1101 120-121 1.057278200155089 1102 1 0.45961594901098834 1102 2 0.7413940296750035 1102 3 0.8089144859913873 1102 4 1.0261734157947444 1102 5 0.9837831672732875 1102 6 1.094465891171005 1102 7 1.0664067801009978 1102 8 0.9634636427545757 1102 9 1.106845045379579 1102 10-11 0.960391505845898 1102 12-13 0.7654575384392643 1102 14-15 1.015238009091867 1102 16-17 1.0340542548173985 1102 18-19 0.9606132653032518 1102 20-21 1.0862059279469065 1102 22-23 1.0612978226510776 1102 24-25 0.9342296298070281 1102 26-27 1.0941000758314416 1102 28-29 1.0121865185034196 1102 30-31 1.120682205875319 1102 32-33 0.9830833555227301 1102 34-35 0.8425377963878589 1102 36-37 0.9987387722048382 1102 38-39 1.1320468696110382 1102 40-41 1.049241566369595 1102 42-43 1.0635728476177846 1102 44-45 1.2666196822831566 1102 46-47 1.0805729050706603 1102 48-49 1.1415054663321982 1102 50-51 1.0102903305270559 1102 52-53 1.0559155985562327 1102 54-55 1.1262754500992784 1102 56-57 1.207165710892376 1102 58-59 1.0579474184083395 1102 60-61 1.1743921232169114 1102 62-63 0.9140832858356731 1102 64-65 0.9631478941530389 1102 66-67 0.928041814850399 1102 68-69 0.9103766279025116 1102 70-71 0.7538681090809511 1102 72-73 0.7754355772547044 1102 74-75 0.7636412478954568 1102 76-77 0.9116673296770301 1102 78-79 0.9681717032755905 1102 80-81 0.6948214531432946 1102 82-83 0.5751077651487471 1102 84-85 0.3458546405718117 1102 86-87 0.5135579453670296 1102 88-89 0.7203191324099016 1102 90-91 0.7141651124105444 1102 92-93 0.5244788075247335 1102 94-95 0.722110741981794 1102 96-97 0.6671667504589003 1102 98-99 0.7836617231660306 1102 100-101 0.6266321227590694 1102 102-103 0.5594393695726616 1102 104-105 0.5135674345677792 1102 106-107 0.4260204607029259 1102 108-109 0.2978208307048611 1102 110-111 0.177347801615376 1102 112-113 0.2573052356865091 1102 114-115 0.001583212611986795 1102 116-117 -0.027677612891228165 1102 118-119 -0.1443740728955163 1102 120-121 -0.06804123738307055 1103 1 0.7506439145519437 1103 2 1.0909981593115994 1103 3 0.9922052212301722 1103 4 1.2010926524022878 1103 5 1.2439102155496897 1103 6 1.3993318855662196 1103 7 1.2690211300367764 1103 8 1.0913633404594734 1103 9 1.1766465912363202 1103 10-11 1.0705042015917101 1103 12-13 1.0332388642563046 1103 14-15 1.1294332699997867 1103 16-17 1.3170330455072232 1103 18-19 1.292276528987621 1103 20-21 1.3220421290834352 1103 22-23 1.3082543969209581 1103 24-25 1.2804876737111712 1103 26-27 1.236648071666771 1103 28-29 1.2413447385301453 1103 30-31 1.2881050833225487 1103 32-33 1.406834523186241 1103 34-35 1.588482801058511 1103 36-37 1.4252063792730993 1103 38-39 1.217877637868753 1103 40-41 1.3196541894607918 1103 42-43 1.373142133851033 1103 44-45 1.375182588532759 1103 46-47 1.2495739527243046 1103 48-49 1.1180208340813351 1103 50-51 1.113669297339463 1103 52-53 1.3694073016583275 1103 54-55 1.185113324886835 1103 56-57 1.2666259341107136 1103 58-59 1.217509244569193 1103 60-61 1.281925620563726 1103 62-63 1.1493182213546902 1103 64-65 1.309047327511884 1103 66-67 1.2223921479588533 1103 68-69 1.279763937021965 1103 70-71 1.090135940275733 1103 72-73 0.9530838218672351 1103 74-75 1.0002041979324332 1103 76-77 1.0492345108724592 1103 78-79 1.1390112127920524 1103 80-81 1.1232013119208766 1103 82-83 1.1022910120966039 1103 84-85 0.9983851457808903 1103 86-87 1.0385621619296863 1103 88-89 1.087149136821079 1103 90-91 0.9096674590898353 1103 92-93 1.0429649885514998 1103 94-95 1.020664866428774 1103 96-97 1.0325612990920838 1103 98-99 1.0272091659829528 1103 100-101 1.1282305649013402 1103 102-103 1.0094232331117219 1103 104-105 1.0982088483488006 1103 106-107 1.1081802246402894 1103 108-109 0.8022875490514494 1103 110-111 0.9098752173814262 1103 112-113 0.9692298776030377 1103 114-115 0.912915589650531 1103 116-117 1.0412240617328443 1103 118-119 0.9829829450865013 1103 120-121 1.1866888134102922 1104 1 0.7418615381113476 1104 2 0.8603361424121161 1104 3 1.0031189234786204 1104 4 1.0313849109670201 1104 5 1.2236523538731419 1104 6 1.062991014704032 1104 7 1.1525608063709285 1104 8 1.063390818546651 1104 9 1.1756121384983729 1104 10-11 1.1272471579500447 1104 12-13 1.0748744576436948 1104 14-15 1.1972938213355846 1104 16-17 1.193966022497115 1104 18-19 1.1239693437503604 1104 20-21 1.171630688750703 1104 22-23 1.2312344831924307 1104 24-25 1.0815125991353014 1104 26-27 1.0072108484516633 1104 28-29 1.0413432190878567 1104 30-31 1.2572413091264565 1104 32-33 1.2807442206775974 1104 34-35 1.3377516207885876 1104 36-37 1.1895338987548172 1104 38-39 1.098454196405882 1104 40-41 1.223760543850581 1104 42-43 1.1827734678720816 1104 44-45 1.2279246069434677 1104 46-47 1.213524979045971 1104 48-49 1.5080247107124691 1104 50-51 1.1515606974603614 1104 52-53 1.0991995405113713 1104 54-55 1.3658318896791357 1104 56-57 1.4006200058218212 1104 58-59 1.424510054390872 1104 60-61 1.5900071234482027 1104 62-63 1.4874210292840502 1104 64-65 1.391661252830886 1104 66-67 1.4844908098640524 1104 68-69 1.3464984584222854 1104 70-71 1.0832768903131829 1104 72-73 1.0672641607767837 1104 74-75 1.1968175663963692 1104 76-77 1.3030526229530892 1104 78-79 1.335850649629247 1104 80-81 1.2317168003764003 1104 82-83 1.2396237502465972 1104 84-85 1.216860003528236 1104 86-87 1.1802201773573664 1104 88-89 1.2964670961361584 1104 90-91 1.2893040167070886 1104 92-93 1.1422800486064304 1104 94-95 1.1551638447065002 1104 96-97 1.0668799565293021 1104 98-99 1.2344212604150613 1104 100-101 1.2454508150426093 1104 102-103 0.964010388321519 1104 104-105 0.9841862029094308 1104 106-107 0.9672349097789947 1104 108-109 0.9652485560556663 1104 110-111 1.1933889810646185 1104 112-113 1.1795147360731484 1104 114-115 1.1354381184624458 1104 116-117 1.0992710691707224 1104 118-119 1.2531038516268396 1104 120-121 0.9137146406439527 1105 1 0.7672601764413152 1105 2 0.8920935974868698 1105 3 0.8745119900915661 1105 4 1.2339442545816226 1105 5 1.247759832319474 1105 6 1.2769839079136247 1105 7 1.3292615723675567 1105 8 1.2846291083050687 1105 9 1.492064104395432 1105 10-11 1.3018361574509782 1105 12-13 1.1660275566563456 1105 14-15 1.4297192497763191 1105 16-17 1.428039948268328 1105 18-19 1.5336899212676087 1105 20-21 1.5052841759021547 1105 22-23 1.5016889822935653 1105 24-25 1.5102151416214156 1105 26-27 1.3768921500673628 1105 28-29 1.4237989702228262 1105 30-31 1.516027502290136 1105 32-33 1.5718341768938728 1105 34-35 1.6518701752389475 1105 36-37 1.2572690120185044 1105 38-39 1.3734364960274021 1105 40-41 1.4830724413769403 1105 42-43 1.5629291063323336 1105 44-45 1.4728759928175421 1105 46-47 1.5563110706483982 1105 48-49 1.6205718544894978 1105 50-51 1.332016732467359 1105 52-53 1.6067791734839787 1105 54-55 1.5219706639763118 1105 56-57 1.7903686042556899 1105 58-59 1.7163852372739612 1105 60-61 1.7938395207391764 1105 62-63 1.60323103648075 1105 64-65 1.6534682689653906 1105 66-67 1.6143474877870894 1105 68-69 1.495468007111537 1105 70-71 1.2201211074195122 1105 72-73 1.1828516905531714 1105 74-75 1.4069765222467758 1105 76-77 1.1982657579866896 1105 78-79 1.4521128957729346 1105 80-81 1.5118466739118297 1105 82-83 1.285864922429969 1105 84-85 1.2193341022865702 1105 86-87 1.2291845647369648 1105 88-89 1.2589649785423802 1105 90-91 1.3513648111075653 1105 92-93 1.2681949843959934 1105 94-95 1.4044578066818012 1105 96-97 1.4120154846124429 1105 98-99 1.325606409495741 1105 100-101 1.3267181330055067 1105 102-103 1.293368542004508 1105 104-105 1.42735685370479 1105 106-107 1.5837824847751136 1105 108-109 1.3531665544997828 1105 110-111 1.4887476317317834 1105 112-113 1.4148028183177814 1105 114-115 1.4994756944616867 1105 116-117 1.3927468823995746 1105 118-119 1.4699233943431267 1105 120-121 1.1905023580589216 1106 1 0.6653749009693435 1106 2 0.9138667123741975 1106 3 0.8024120440533267 1106 4 0.9567257988778053 1106 5 0.989807852643473 1106 6 0.8784161737475813 1106 7 1.0125103742905566 1106 8 0.8111365754754374 1106 9 0.9771877193732692 1106 10-11 1.0099097572995603 1106 12-13 0.6638475970039366 1106 14-15 0.9790239331623027 1106 16-17 1.0351559981805707 1106 18-19 1.1244859028311822 1106 20-21 1.0547026000884898 1106 22-23 1.1423783210036902 1106 24-25 0.9449535356099972 1106 26-27 1.0661325308298828 1106 28-29 1.0382291613724846 1106 30-31 1.1108235133742212 1106 32-33 1.1230583448169753 1106 34-35 1.0951111281784804 1106 36-37 1.0987926959354226 1106 38-39 1.1020861105459332 1106 40-41 1.1251431664485025 1106 42-43 1.0970839058251016 1106 44-45 0.8995327122289751 1106 46-47 1.1093796194503653 1106 48-49 1.0835863416812828 1106 50-51 0.9605590312383967 1106 52-53 1.1848772102396268 1106 54-55 1.1602115366026808 1106 56-57 1.3337103610648384 1106 58-59 1.2464238787573194 1106 60-61 1.3877503505564661 1106 62-63 1.3227822552221014 1106 64-65 1.2436859957730064 1106 66-67 1.194458345996157 1106 68-69 1.0932162865648714 1106 70-71 1.0853669112739368 1106 72-73 1.0785793777121384 1106 74-75 1.068702265393135 1106 76-77 1.17402442431937 1106 78-79 1.2251379191831369 1106 80-81 1.4295115752807526 1106 82-83 0.9889237251213707 1106 84-85 0.9814621492484967 1106 86-87 1.1314028576438346 1106 88-89 1.1970704475213125 1106 90-91 1.034044909028843 1106 92-93 0.9342641500898559 1106 94-95 1.0138046088939277 1106 96-97 0.8813167557744208 1106 98-99 0.9707162279749753 1106 100-101 1.016898611871639 1106 102-103 1.0508798846104668 1106 104-105 1.0956077155518287 1106 106-107 1.0541100278913405 1106 108-109 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1.2903230090560704 2210 68-69 1.0238572452806771 2210 70-71 1.2203764691153296 2210 72-73 1.227362547834236 2210 74-75 1.3180903167452165 2210 76-77 1.3308999749422732 2210 78-79 1.007767888624965 2210 80-81 1.0775592104254947 2210 82-83 0.9867444176236226 2210 84-85 1.17091849653767 2210 86-87 1.067725609045727 2210 88-89 1.2535321989546162 2210 90-91 1.234974405365005 2210 92-93 1.2401300321858812 2210 94-95 1.282452682891666 2210 96-97 1.2737570117869623 2210 98-99 0.9750567070038159 2210 100-101 1.0721869475657648 2210 102-103 1.01304096639657 2210 104-105 1.0259777565802466 2210 106-107 0.9575670422849427 2210 108-109 1.1998419215799707 2210 110-111 0.8893782797422247 2210 112-113 1.1639958825573586 2210 114-115 1.02924197822378 2210 116-117 1.0709933051763265 2210 118-119 0.9894606336006575 2210 120-121 0.901064722459882 2211 1 0.19859354895115544 2211 2 0.5531325585258315 2211 3 0.5353570274071906 2211 4 0.8019820990900541 2211 5 1.0100798652277376 2211 6 0.8622868402753809 2211 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0.4200623426548624 2211 72-73 0.37002628066893806 2211 74-75 0.5550402232516518 2211 76-77 0.5099041196250482 2211 78-79 0.31723306971663945 2211 80-81 0.5035608420965865 2211 82-83 0.5386768164183309 2211 84-85 0.4866197578075173 2211 86-87 0.7040538131356264 2211 88-89 0.728419053878806 2211 90-91 0.6837735788104489 2211 92-93 0.9662498706861733 2211 94-95 0.9717405239645167 2211 96-97 0.9379924488247049 2211 98-99 1.0927451531050743 2211 100-101 1.1475531803111423 2211 102-103 1.1003821166651413 2211 104-105 1.1550468108065104 2211 106-107 1.1229110033153944 2211 108-109 1.1457210588189923 2211 110-111 1.2698518216595005 2211 112-113 1.1450637338728171 2211 114-115 1.160690949794592 2211 116-117 1.3238481340890615 2211 118-119 1.2528287243072782 2211 120-121 1.3288287375617713 2212 1 -0.1307192975699003 2212 2 0.05280519945008777 2212 3 0.1255674061654659 2212 4 0.1309285111198193 2212 5 0.32321931527542347 2212 6 0.24822433600577654 2212 7 0.1600673351820916 2212 8 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-0.9304429831414076 2316 108-109 -1.2258882184226785 2316 110-111 -1.2296520489075249 2316 112-113 -1.68809816338673 2316 114-115 -1.4865845042963954 2316 116-117 -1.1006818684862658 2316 118-119 -1.1988323624812836 2316 120-121 -1.3182406902608648 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27704.0 3 4828.0 4 4439.0 5 4710.0 6 5471.0 7 6096.0 8 7215.0 9 8241.0 10 10044.0 11 11818.0 12 12073.0 13 13293.0 14 16015.0 15 16429.0 16 13782.0 17 14299.0 18 15036.0 19 15610.0 20 17175.0 21 18859.0 22 20468.0 23 22816.0 24 25457.0 25 28934.0 26 33114.0 27 37816.0 28 43188.0 29 50041.0 30 58105.0 31 68269.0 32 83132.0 33 102226.0 34 129096.0 35 165935.0 36 217749.0 37 236029.0 38 82488.0 39 421.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.21300612214388 17.61912170267797 12.66233647847283 20.50553569670532 2 13.578232476290497 23.776351892042257 36.78125494011811 25.864160691549138 3 25.70551173088937 24.274042207782255 29.882087227127847 20.138358834200528 4 10.891434385035915 15.116814983697385 43.60110073300483 30.390649898261874 5 10.585853533858366 40.60405132156147 34.3082542198457 14.501840924734466 6 25.672544302959416 37.142333448581105 18.514002604109194 18.671119644350284 7 25.572400961656903 33.06807895980247 23.79438976275412 17.565130315786508 8 22.500366131124576 28.585679891710974 20.18231185302792 28.73164212413653 9 21.470923610179263 21.858653740695058 26.998283767232024 29.672138881893652 10-11 25.086911199406714 23.50494204541591 28.17356447571924 23.23458227945814 12-13 30.46506670064593 22.07360717681307 26.559972996285154 20.901353126255845 14-15 23.53016491460217 25.08040505770786 30.885588963218897 20.503841064471075 16-17 19.40939814458783 28.638172566996655 30.631795449371708 21.320633839043808 18-19 18.170867985040637 33.32831704726077 28.300833725134904 20.199981242563688 20-21 21.334188924446586 27.727771255632057 25.771127538542203 25.166912281379155 22-23 19.05045797639931 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22.373021953297282 24.806518322289854 31.584511173308428 21.235948551104432 74-75 19.171939692211545 31.9016743483582 27.85863849484798 21.067747464582272 76-77 20.78854232637175 27.08384079878202 27.237037915367175 24.890578959479058 78-79 22.146814621145577 27.546218389425768 28.56181263769489 21.745154351733763 80-81 24.49055704494409 26.166050140577763 27.541709439090585 21.801683375387558 82-83 22.19654622568986 25.216650807060688 31.423891990254777 21.162910976994674 84-85 21.094664314778537 28.61977628037221 29.868836143447187 20.416723261402066 86-87 20.930576152399823 30.151644542641755 27.58697215456512 21.3308071503933 88-89 19.491843366647274 29.35027086606643 25.556276203081197 25.6016095642051 90-91 21.122797853285352 26.129019303816776 26.474961995352075 26.273220847545797 92-93 23.60883114845142 28.550530170495573 27.352083719002895 20.488554962050117 94-95 22.183493560274506 27.115345008936913 28.483955239433236 22.217206191355345 96-97 17.514472712649336 28.388892141317797 32.25872401049618 21.83791113553668 98-99 20.286115294307407 30.09846789221797 27.327040293303135 22.28837652017149 100-101 18.702503527455285 27.178973975014397 28.713945911739113 25.404576585791204 102-103 22.069157447992765 25.3708081507452 28.50471285642959 24.055321544832438 104-105 25.443330597916386 25.54178722215038 27.23010535747446 21.784776822458774 106-107 21.63693874411555 26.7047819022806 31.375905459016163 20.282373894587685 108-109 19.69814389722441 27.374474580208087 30.596140215723572 22.331241306843932 110-111 18.748023465903103 31.56154161079255 27.489268492391723 22.201166430912625 112-113 21.07323019894144 27.464197619395662 25.617560611451427 25.84501157021146 114-115 19.15696248075738 27.656722972755542 27.302226612265812 25.884087934221267 116-117 23.154409021216633 26.848457553086043 26.003569268238696 23.99356415745863 118-119 22.066194752122236 24.77508438359356 30.397468952049273 22.761251912234933 120-121 19.519677729690997 31.00172211357491 27.106815481307606 22.37178467542649 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4222.0 1 5449.0 2 3976.0 3 1219.0 4 1052.0 5 968.0 6 1046.5 7 1197.5 8 1391.5 9 1462.5 10 1429.0 11 1369.0 12 1397.5 13 1552.0 14 1670.5 15 1760.5 16 1845.0 17 1952.0 18 2166.5 19 2407.5 20 2664.5 21 2983.5 22 3441.5 23 4222.0 24 5217.0 25 6480.0 26 8119.5 27 10047.0 28 12622.5 29 15722.5 30 18709.5 31 21593.0 32 24397.0 33 27192.5 34 29892.0 35 32642.0 36 35587.0 37 38726.0 38 41460.0 39 43354.5 40 44981.0 41 46088.0 42 46894.0 43 48355.0 44 49498.5 45 51606.0 46 59887.0 47 99881.5 48 191978.5 49 194639.0 50 105735.5 51 60198.0 52 42214.0 53 33774.5 54 28924.0 55 24908.5 56 20991.5 57 17267.5 58 13995.0 59 11337.5 60 8991.5 61 6858.5 62 5166.0 63 3765.5 64 2625.0 65 1805.0 66 1165.0 67 761.5 68 491.0 69 336.5 70 238.0 71 148.5 72 97.5 73 64.5 74 44.0 75 34.0 76 34.0 77 28.0 78 16.5 79 13.0 80 12.0 81 8.5 82 5.5 83 5.5 84 5.5 85 6.5 86 6.0 87 3.0 88 3.0 89 4.0 90 3.5 91 2.5 92 3.5 93 2.0 94 0.0 95 1.0 96 2.0 97 1.5 98 2.5 99 3.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17707854971515166 2 0.9933748720745489 3 1.049489177825325 4 1.0180045024905653 5 1.0254661885525602 6 1.040571553019526 7 1.0103001599712695 8 1.000472573450593 9 0.9923435821310213 10-11 0.9798164425228749 12-13 0.9738410272618464 14-15 0.9743263401764476 16-17 1.0005029055077557 18-19 1.035415103301887 20-21 1.0191874527199059 22-23 0.9918582692164198 24-25 0.9794221257797614 26-27 0.9823340032673692 28-29 1.0164575675752736 30-31 1.0214926890642622 32-33 1.0098755111709934 34-35 1.0182471589478659 36-37 1.032139241128328 38-39 1.01512295706012 40-41 1.027589432553941 42-43 1.0210377082068234 44-45 0.9949218069898406 46-47 0.9894620367005759 48-49 0.9913426242446559 50-51 0.9631641431406176 52-53 0.9887037352715113 54-55 1.0031114624237376 56-57 1.0613186801187318 58-59 1.0312292794134508 60-61 1.030652970327362 62-63 0.9968327265910832 64-65 0.9870051400704069 66-67 0.9897956893293643 68-69 1.00678164134041 70-71 0.9765102482921536 72-73 0.9803624195518014 74-75 0.9884307467570481 76-77 1.0031721265380629 78-79 1.1335393082228387 80-81 1.103146586945932 82-83 1.1219221303295699 84-85 1.0722078886400987 86-87 1.0457280027371647 88-89 1.041724171191704 90-91 1.053765997885249 92-93 1.0348691262729606 94-95 1.0303799818128985 96-97 1.0410871979912897 98-99 1.0852506732200087 100-101 1.070236304924531 102-103 1.0364767253025775 104-105 1.0153959455745831 106-107 1.0185504795194917 108-109 1.0160935828893225 110-111 1.0177618460332647 112-113 1.0228272995794156 114-115 1.0283477339830056 116-117 1.064928194921079 118-119 1.0601660619465536 120-121 1.0310169550133128 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1648421.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.395363543999416 #Duplication Level Percentage of deduplicated Percentage of total 1 84.19398115709006 44.955682320994605 2 10.389553123241704 11.095079321503707 3 2.3215979889947187 3.7188770587617284 4 0.9605426137882902 2.051540882509167 5 0.5345764446130055 1.4271951801085048 6 0.3208404665962307 1.0278836012119286 7 0.21979361274846554 0.8215171900147341 8 0.16132908143648936 0.6891379962816668 9 0.12473993350623186 0.5994480688217607 >10 0.6925766092308251 6.625118632146387 >50 0.04532614694123899 1.6718560681841097 >100 0.026005745930483565 2.6269203037943742 >500 0.0026942659653279846 1.0085303364233726 >1k 0.004685679939700843 5.806742887137202 >5k 9.371359879401685E-4 3.7216921078843406 >10k+ 8.199939894476475E-4 12.15277804422245 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 56523 3.428917734001205 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 49036 2.9747255100487076 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 30554 1.8535313490910394 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 20860 1.2654534248229063 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 13708 0.8315836791693385 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 12769 0.7746200758180101 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 10844 0.6578416557420708 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9562 0.5800702611772114 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9212 0.5588378211634043 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9019 0.5471296470986478 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7974 0.4837356476288521 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7424 0.45037038475001223 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6156 0.3734482877857052 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5084 0.30841635722913013 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5070 0.3075670596285779 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4991 0.30277459459689 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4484 0.2720178886340322 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4397 0.266740110687743 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4278 0.25952108108304855 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4219 0.2559418983378639 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4053 0.24587165535988684 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3960 0.24022989272764664 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3854 0.23379949660917934 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3782 0.22943168037776757 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3640 0.22081737614359437 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3215 0.19503512755539998 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3147 0.19090996778128888 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2771 0.1681002607950275 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2329 0.1412867222633053 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2185 0.13255108980048178 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2178 0.13212644100020565 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2147 0.1302458534561256 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1928 0.11696041241891482 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1913 0.11605045070403738 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1895 0.11495849664618443 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1892 0.11477650430320895 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1692 0.10264368143817629 No Hit ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1670 0.1013090709230227 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1651 0.10015645275084459 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5166028581290822E-4 0.0 12-13 0.0 0.0 0.0 2.4265645730065316E-4 0.0 14-15 0.0 3.0332057162581645E-5 0.0 4.24648800276143E-4 0.0 16-17 0.0 6.066411432516329E-5 0.0 5.15644971763888E-4 0.0 18-19 0.0 6.066411432516329E-5 0.0 7.279693719019595E-4 0.0 20-21 0.0 6.066411432516329E-5 0.0 0.0013649425723161741 0.0 22-23 0.0 6.066411432516329E-5 0.0 0.0020929119442181336 0.0 24-25 0.0 6.066411432516329E-5 0.0 0.0030332057162581645 0.0 26-27 0.0 6.066411432516329E-5 0.0 0.004034163602623359 0.0 28-29 0.0 6.066411432516329E-5 0.0 0.00682471286158087 0.0 30-31 0.0 6.066411432516329E-5 0.0 0.013224776922885598 0.0 32-33 0.0 6.066411432516329E-5 0.0 0.027056194989022828 0.0 34-35 0.0 6.066411432516329E-5 0.0 0.040675288655021985 0.0 36-37 0.0 9.099617148774494E-5 0.0 0.04765166180241577 0.0 38-39 0.0 1.5166028581290822E-4 0.0 0.05450670672115922 0.0 40-41 0.0 1.8199234297548988E-4 0.0 0.06767081952971965 0.0 42-43 0.0 1.8199234297548988E-4 0.0 0.08450511125495247 0.0 44-45 0.0 1.8199234297548988E-4 0.0 0.09991379629354394 0.0 46-47 0.0 1.8199234297548988E-4 0.0 0.11556513778943607 0.0 48-49 0.0 1.8199234297548988E-4 0.0 0.1254230563672751 0.0 50-51 0.0 2.4265645730065316E-4 0.0 0.14265166483562147 0.0 52-53 0.0 2.4265645730065316E-4 0.0 0.22148468140117117 0.0 54-55 0.0 2.4265645730065316E-4 0.0 0.3115708911740387 0.0 56-57 0.0 2.4265645730065316E-4 0.0 0.3631960524647526 0.0 58-59 0.0 2.4265645730065316E-4 0.0 0.47563698836644286 0.0 60-61 0.0 2.4265645730065316E-4 0.0 0.6090373757674769 0.0 62-63 0.0 2.4265645730065316E-4 0.0 0.6527762021959196 0.0 64-65 0.0 3.0332057162581644E-4 0.0 0.6905092813061713 0.0 66-67 0.0 3.336526287883981E-4 0.0 0.9398084591254298 0.0 68-69 0.0 3.6398468595097975E-4 0.0 1.1221041226725454 0.0 70-71 0.0 3.6398468595097975E-4 0.0 1.160413510868886 0.0 72-73 0.0 3.6398468595097975E-4 0.0 1.2392768594915982 0.0 74-75 0.0 3.6398468595097975E-4 0.0 1.360878076656388 0.0 76-77 0.0 3.6398468595097975E-4 0.0 1.4633094336944263 0.0 78-79 0.0 3.943167431135614E-4 0.0 1.5601900242717122 0.0 80-81 0.0 4.2464880027614306E-4 0.0 1.7388458409593182 0.0 82-83 0.0 4.2464880027614306E-4 0.0 1.930696102512647 0.0 84-85 0.0 4.2464880027614306E-4 0.0 2.1299473860136455 0.0 86-87 0.0 4.2464880027614306E-4 0.0 2.5819860339076 0.0 88-89 0.0 4.2464880027614306E-4 0.0 3.241313960450637 0.0 90-91 0.0 4.2464880027614306E-4 0.0 3.989363154194226 0.0 92-93 0.0 4.2464880027614306E-4 0.0 4.776935018420659 0.0 94-95 0.0 4.2464880027614306E-4 0.0 5.457070736177227 0.0 96-97 0.0 4.2464880027614306E-4 0.0 6.13068506164384 0.0 98-99 0.0 4.2464880027614306E-4 0.0 6.808697535399027 0.0 100-101 0.0 4.2464880027614306E-4 0.0 7.514585169686628 0.0 102-103 0.0 4.2464880027614306E-4 0.0 8.377441199790589 0.0 104-105 0.0 4.2464880027614306E-4 0.0 9.171686116592788 0.0 106-107 0.0 4.2464880027614306E-4 0.0 9.944516601038206 0.0 108-109 0.0 4.2464880027614306E-4 0.0 10.650252575039993 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2480 0.0 66.58681 2 ACCTGGG 2820 0.0 57.478474 3 GTACCTG 3080 0.0 54.78497 1 TATAACG 295 0.0 54.612743 2 AGTACGC 1350 0.0 43.473476 2 GTACCGG 120 5.7661964E-10 43.192245 1 GTACGCA 1370 0.0 42.794186 3 ATAACGC 390 0.0 41.266594 3 TACGCAG 1535 0.0 39.310295 4 GAGTACG 1535 0.0 39.018406 1 CCTGGGG 1720 0.0 38.42331 4 ACGCAGT 1630 0.0 37.724327 5 CGCAGTG 1810 0.0 33.65524 6 GTATAAC 615 0.0 32.7746 1 ACACGGG 230 1.2732926E-11 29.988768 3 TAACGCA 505 0.0 29.587435 4 GTAGGCA 910 0.0 29.055052 3 CGCGCGA 40 0.0063534128 28.736595 110-111 GTACCCG 230 3.8926373E-10 27.542883 1 CCTGGGA 1720 0.0 27.063374 4 >>END_MODULE