##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003786_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1648421 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71732039327332 31.0 31.0 34.0 30.0 34.0 2 31.883689300245507 31.0 31.0 34.0 30.0 34.0 3 31.950953063568104 31.0 31.0 34.0 30.0 34.0 4 35.64950215994579 37.0 35.0 37.0 33.0 37.0 5 35.53219171558722 37.0 35.0 37.0 33.0 37.0 6 35.62187147579411 37.0 35.0 37.0 33.0 37.0 7 35.582525944525095 37.0 35.0 37.0 33.0 37.0 8 35.593723933388375 37.0 35.0 37.0 33.0 37.0 9 37.16591756596161 39.0 37.0 39.0 33.0 39.0 10-11 37.05989246679095 39.0 37.0 39.0 33.0 39.0 12-13 37.04317252692122 39.0 37.0 39.0 33.0 39.0 14-15 38.079251295633824 40.0 37.0 41.0 33.0 41.0 16-17 38.151081550162246 40.0 37.0 41.0 33.0 41.0 18-19 38.2195376666519 40.0 37.5 41.0 33.0 41.0 20-21 38.14476520257871 40.0 37.5 41.0 33.0 41.0 22-23 38.042323532641234 40.0 37.0 41.0 33.0 41.0 24-25 37.89181980816794 40.0 37.0 41.0 32.5 41.0 26-27 37.652483194523725 39.5 36.5 41.0 32.5 41.0 28-29 37.53638936897795 39.0 37.0 41.0 32.0 41.0 30-31 37.36579399316073 39.0 36.0 41.0 31.5 41.0 32-33 37.088961193772704 39.0 36.0 40.5 31.0 41.0 34-35 36.986766426780534 39.0 36.0 40.5 31.0 41.0 36-37 36.78658091591893 39.0 35.5 40.5 30.5 41.0 38-39 36.55593110012552 39.0 35.0 40.0 30.0 41.0 40-41 36.690114357921914 39.0 35.0 41.0 30.0 41.0 42-43 36.56441133666703 39.0 35.0 41.0 30.0 41.0 44-45 36.32178521142354 39.0 35.0 41.0 30.0 41.0 46-47 36.16181424526866 39.0 35.0 41.0 29.5 41.0 48-49 35.896636842165925 39.0 35.0 41.0 28.0 41.0 50-51 35.61600343601543 39.0 35.0 40.0 28.0 41.0 52-53 35.49227624496412 39.0 35.0 40.0 27.0 41.0 54-55 35.32086372352694 38.5 34.5 40.0 27.0 41.0 56-57 34.96350173893684 38.0 34.0 40.0 26.0 41.0 58-59 34.67905165003358 38.0 34.0 40.0 26.0 41.0 60-61 34.167394130504285 38.0 33.0 40.0 24.0 41.0 62-63 33.99439918564493 37.0 33.0 40.0 24.0 41.0 64-65 33.566399299693465 36.5 32.5 39.5 22.5 41.0 66-67 33.11600464929772 36.0 32.0 39.0 22.0 40.0 68-69 32.7234984266762 36.0 32.0 39.0 22.0 40.0 70-71 32.18796775823652 35.0 31.0 37.5 21.0 40.0 72-73 31.72482029772734 35.0 31.0 37.0 20.5 39.5 74-75 31.38984488792608 35.0 31.0 37.0 18.5 39.0 76-77 30.391338438420767 34.0 29.5 35.5 18.0 38.0 78-79 30.81232646271796 34.0 31.0 36.0 18.0 38.0 80-81 30.51686007397382 34.0 30.0 35.5 16.0 37.0 82-83 30.152251457607008 34.0 30.0 35.0 12.5 37.0 84-85 29.806992267145347 34.0 30.0 35.0 7.5 36.5 86-87 29.661099925322475 34.0 30.0 35.0 7.0 36.0 88-89 29.318318257289853 34.0 29.0 35.0 2.0 36.0 90-91 28.94536256211247 34.0 29.0 35.0 2.0 35.5 92-93 28.73284130692341 34.0 29.0 35.0 2.0 35.0 94-95 28.354134350387433 34.0 29.0 35.0 2.0 35.0 96-97 27.919519042768805 34.0 29.0 35.0 2.0 35.0 98-99 27.6402263135449 34.0 29.0 35.0 2.0 35.0 100-101 26.662019593295646 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24517665080635354 1101 2 0.24653319938715867 1101 3 0.23428006768664744 1101 4 0.20050180541392137 1101 5 0.10566687789712148 1101 6 0.20330201992585728 1101 7 0.24035542303764856 1101 8 0.22133290196156707 1101 9 0.34444629055069953 1101 10-11 0.324474851922794 1101 12-13 0.37924631558687594 1101 14-15 0.38048881169601856 1101 16-17 0.5111553452119324 1101 18-19 0.4571396850686824 1101 20-21 0.36209497412293956 1101 22-23 0.2931571826777599 1101 24-25 0.36200505215916934 1101 26-27 0.4237148434225517 1101 28-29 0.5068073149092385 1101 30-31 0.4576889775215207 1101 32-33 0.4358610276135124 1101 34-35 0.48627642227279466 1101 36-37 0.5560014548741279 1101 38-39 0.32367369114573563 1101 40-41 0.5128219427504419 1101 42-43 0.4771235544720085 1101 44-45 0.4581077626760788 1101 46-47 0.3824701800845176 1101 48-49 0.39539953044395304 1101 50-51 0.4413862842238032 1101 52-53 0.32749935049425716 1101 54-55 0.38411069210895477 1101 56-57 0.3607209776359994 1101 58-59 0.364370471377903 1101 60-61 0.17597254098860304 1101 62-63 0.26352164859455485 1101 64-65 0.12225323262734378 1101 66-67 0.11054012333535468 1101 68-69 0.45059513384198624 1101 70-71 0.2635990232908796 1101 72-73 0.2135046167623038 1101 74-75 0.15603040870774976 1101 76-77 0.290523917231976 1101 78-79 0.4711532651386712 1101 80-81 0.27295791946115244 1101 82-83 0.411458887573275 1101 84-85 0.4799139755268911 1101 86-87 0.2116833371837643 1101 88-89 0.33616616705193536 1101 90-91 0.2986498651398328 1101 92-93 0.511216273092554 1101 94-95 0.4850179525932923 1101 96-97 0.42135643806252787 1101 98-99 0.3795342340920165 1101 100-101 0.3726796658650322 1102 1 0.07974498526217388 1102 2 0.15164923546628728 1102 3 0.16507620674111223 1102 4 0.09336945756375314 1102 5 0.09841540974331053 1102 6 0.02161326538606545 1102 7 0.03333904832324919 1102 8 0.1131010123390368 1102 9 0.05502447249476461 1102 10-11 0.08918628810138074 1102 12-13 0.04610856562306509 1102 14-15 -0.0610094683767457 1102 16-17 0.2173379171404477 1102 18-19 0.05850189733886424 1102 20-21 0.12658315808119625 1102 22-23 0.11806607216811216 1102 24-25 0.18157850863069314 1102 26-27 -0.10274457699501482 1102 28-29 -0.014823563213539614 1102 30-31 0.13151945060379688 1102 32-33 0.04041859875524523 1102 34-35 0.1344954399787568 1102 36-37 0.1275171073135013 1102 38-39 -0.06452081012258049 1102 40-41 -0.1039360180106712 1102 42-43 0.23056478583290385 1102 44-45 0.11416244157054933 1102 46-47 0.05419500116802567 1102 48-49 0.1586420260221857 1102 50-51 0.31164428904526176 1102 52-53 0.12184038861905577 1102 54-55 0.07366500593193592 1102 56-57 0.3253151424720713 1102 58-59 -0.01413783399809887 1102 60-61 0.04235736565988191 1102 62-63 -0.24902414269785567 1102 64-65 -0.47616244373049454 1102 66-67 -0.3084761664055051 1102 68-69 -0.10085633997672261 1102 70-71 -0.18465268562127335 1102 72-73 -0.26192131306844146 1102 74-75 -0.21794951550844743 1102 76-77 -0.16104707028636156 1102 78-79 -0.1677430545632319 1102 80-81 -0.18237865440602619 1102 82-83 -0.3707972226531311 1102 84-85 -0.23755394696465615 1102 86-87 -0.2940250352269018 1102 88-89 -0.38128995356012396 1102 90-91 -0.4321366750526501 1102 92-93 -0.4545527688466038 1102 94-95 -0.39166519211977757 1102 96-97 -0.34539652656960484 1102 98-99 -0.33049634064378 1102 100-101 -0.1547848812889825 1103 1 -0.01015902306715688 1103 2 0.05610962896889049 1103 3 0.04492363204307992 1103 4 0.0070852061009318845 1103 5 0.10490283465606609 1103 6 0.11803226846090809 1103 7 0.06144933089782256 1103 8 0.12681254431355882 1103 9 0.16094331015061414 1103 10-11 0.14545510045785903 1103 12-13 0.12227205310961153 1103 14-15 0.056095626203997995 1103 16-17 0.12124387319738616 1103 18-19 -0.08280525330270194 1103 20-21 -0.00905008043353206 1103 22-23 0.11125490443973973 1103 24-25 0.17481761530324036 1103 26-27 0.021146142674929536 1103 28-29 -0.05775749616370973 1103 30-31 -0.10601391700262752 1103 32-33 0.11009445860967304 1103 34-35 0.04211156573612129 1103 36-37 -0.1766276116039407 1103 38-39 -0.09352549050712611 1103 40-41 -0.049087660199646166 1103 42-43 0.2531799547548914 1103 44-45 0.3075123141006131 1103 46-47 0.22737571655653 1103 48-49 0.2254283241396564 1103 50-51 0.26704866551295936 1103 52-53 0.28924035877876264 1103 54-55 0.2808701384617436 1103 56-57 0.2578750744232323 1103 58-59 0.2148926728327396 1103 60-61 0.25437221769097107 1103 62-63 0.3214942188107628 1103 64-65 0.4146450036922076 1103 66-67 0.12866562848790863 1103 68-69 0.14145755554334016 1103 70-71 0.3022084645921552 1103 72-73 0.3810479335939867 1103 74-75 0.17543773847133792 1103 76-77 0.052612849339471524 1103 78-79 0.23824320755127815 1103 80-81 0.20794794269817274 1103 82-83 0.35956393404731557 1103 84-85 0.3145396574828432 1103 86-87 0.43506811188572314 1103 88-89 0.23991250469590497 1103 90-91 0.30348038645727016 1103 92-93 0.3970517954202961 1103 94-95 0.48823514293478 1103 96-97 0.3626545538434911 1103 98-99 0.535159107803878 1103 100-101 0.5508627584089822 1104 1 0.08175532638157179 1104 2 0.03624394766739414 1104 3 0.09579291729742323 1104 4 -0.02586739058487808 1104 5 0.05829250507670736 1104 6 0.08712516962653183 1104 7 0.10823131639001815 1104 8 0.007498133101549342 1104 9 -0.07612623595500878 1104 10-11 0.07464539376771029 1104 12-13 0.10837346807997505 1104 14-15 0.013995781597770929 1104 16-17 0.16279712923901002 1104 18-19 0.08665486010518464 1104 20-21 0.005403717022346655 1104 22-23 0.1517301777750788 1104 24-25 0.28048106951300156 1104 26-27 0.20539617881301098 1104 28-29 0.3027671467262891 1104 30-31 0.0727240342306672 1104 32-33 0.16405478227552095 1104 34-35 0.1377590206292112 1104 36-37 -0.04929916895837039 1104 38-39 0.19760581230376317 1104 40-41 0.4623929582872819 1104 42-43 0.3611780115114058 1104 44-45 0.4327010124067172 1104 46-47 0.3518057203674587 1104 48-49 0.32373131243091535 1104 50-51 0.10610420482462501 1104 52-53 0.24800433764784202 1104 54-55 0.11461194168658295 1104 56-57 0.2551386409895926 1104 58-59 0.4079351465833483 1104 60-61 0.28849848088137264 1104 62-63 0.12881949116052738 1104 64-65 0.13436190284723182 1104 66-67 0.13019307865020835 1104 68-69 0.18651825931615917 1104 70-71 0.024357703392631436 1104 72-73 0.1969989155643681 1104 74-75 0.11451980642408088 1104 76-77 -0.018371593214112636 1104 78-79 0.02114673035769954 1104 80-81 0.003473513679946194 1104 82-83 0.154202889919425 1104 84-85 0.1682613055110167 1104 86-87 0.35721271022731926 1104 88-89 0.4108425758550851 1104 90-91 0.19361362101316715 1104 92-93 0.28966089958997543 1104 94-95 0.39374041088226264 1104 96-97 0.4993532328321528 1104 98-99 0.5937734421403142 1104 100-101 0.7478693338981586 1105 1 0.01249138324921617 1105 2 -0.008009287828596001 1105 3 0.020359960420584855 1105 4 0.025026036279157893 1105 5 -0.040242723567224914 1105 6 -0.059471425324296945 1105 7 -0.04164479136647259 1105 8 0.048587985259167965 1105 9 0.033447119366613265 1105 10-11 -0.05865250412244194 1105 12-13 -0.06397119418931396 1105 14-15 0.07546635526485801 1105 16-17 0.01500281908342771 1105 18-19 -0.10895898402576165 1105 20-21 0.0066095488027713145 1105 22-23 0.09787503326049318 1105 24-25 0.03262852812990502 1105 26-27 0.04874564401392689 1105 28-29 -0.041369210080041796 1105 30-31 0.035687475092672116 1105 32-33 0.19614522507010435 1105 34-35 0.09689934623293794 1105 36-37 0.03632487708383536 1105 38-39 0.20639543940330185 1105 40-41 0.3243843247481806 1105 42-43 0.2496953878512116 1105 44-45 0.40685637864950763 1105 46-47 0.19992627524154472 1105 48-49 0.23210599520808017 1105 50-51 0.27142782102131235 1105 52-53 0.385584665732317 1105 54-55 0.33408373159131344 1105 56-57 0.15596844638740492 1105 58-59 0.16247699502314816 1105 60-61 0.3402786956667754 1105 62-63 0.38386435917460204 1105 64-65 0.0946117019099546 1105 66-67 0.20443872618853476 1105 68-69 0.11008779610109798 1105 70-71 0.2778366966542052 1105 72-73 0.23537283663212705 1105 74-75 0.36338358988883 1105 76-77 0.21147879956571458 1105 78-79 0.3019076156221985 1105 80-81 0.18896390677611308 1105 82-83 0.3262116508263304 1105 84-85 0.2132866562823601 1105 86-87 0.30451011945798356 1105 88-89 0.3292054334058534 1105 90-91 0.40446789684603957 1105 92-93 0.18432439676852752 1105 94-95 0.147026063149017 1105 96-97 0.17474910320169457 1105 98-99 0.30105392727012514 1105 100-101 0.2700816959840111 1106 1 0.01172124515833417 1106 2 0.034965199067986674 1106 3 -0.032309410267195915 1106 4 0.008927371778483462 1106 5 -0.07532915572235765 1106 6 -0.019012693916252488 1106 7 -0.025296070755118194 1106 8 -0.01665408511860278 1106 9 -0.05031039045655916 1106 10-11 0.02354193794637638 1106 12-13 -0.1005475440039163 1106 14-15 -0.08818929142322673 1106 16-17 -0.0458237639649397 1106 18-19 -0.16142388174804267 1106 20-21 -0.01593196767466054 1106 22-23 0.09441093128970124 1106 24-25 0.010122392855635098 1106 26-27 -0.0161905851659796 1106 28-29 -0.21158839554830422 1106 30-31 -0.05061552779469025 1106 32-33 -0.019937767511130744 1106 34-35 0.12797229098659813 1106 36-37 0.16801284699722885 1106 38-39 0.2422939027303599 1106 40-41 0.0011260241659698522 1106 42-43 0.20238333909721717 1106 44-45 0.19643220794800698 1106 46-47 0.10294965331726047 1106 48-49 0.056030460897645185 1106 50-51 0.16614008935583513 1106 52-53 0.10949450140291361 1106 54-55 0.3072986413605534 1106 56-57 0.22059167130375812 1106 58-59 0.2040711808712743 1106 60-61 -0.013196671852753639 1106 62-63 -0.04719547499825438 1106 64-65 -0.06124900989039617 1106 66-67 -0.10520953255702636 1106 68-69 -0.11460411610315191 1106 70-71 -0.055847337480187775 1106 72-73 0.003728843600701026 1106 74-75 0.01977002729687527 1106 76-77 -0.15391807985557548 1106 78-79 -0.1634767609935608 1106 80-81 -0.03816149419277082 1106 82-83 -0.014003579761435958 1106 84-85 -0.021925628765973926 1106 86-87 0.22233716658618263 1106 88-89 -0.014020889514700485 1106 90-91 0.16532395699495694 1106 92-93 0.23087911412481077 1106 94-95 0.19509544330072615 1106 96-97 0.1007181683087559 1106 98-99 0.2777253418193091 1106 100-101 0.3423624551647819 1107 1 -0.026488210992326344 1107 2 0.0054479580505244485 1107 3 0.06836113204307992 1107 4 -1.0282536165107103E-4 1107 5 0.021901665438377904 1107 6 0.13005660944730835 1107 7 0.2330585996053003 1107 8 0.1332166684632199 1107 9 0.38314781695333977 1107 10-11 0.05559142061091649 1107 12-13 0.07386909817763154 1107 14-15 0.20031464193529303 1107 16-17 0.1834847851871757 1107 18-19 0.19462485936736584 1107 20-21 0.12028133963448795 1107 22-23 0.06895712595333947 1107 24-25 0.2525108019528943 1107 26-27 0.16088094284499022 1107 28-29 0.28519530995874476 1107 30-31 0.30590280281029436 1107 32-33 0.2240533234055917 1107 34-35 0.2476148608041413 1107 36-37 0.1737723139913001 1107 38-39 0.3541985209724672 1107 40-41 0.550827305786747 1107 42-43 0.24150770773108832 1107 44-45 0.5230668953080979 1107 46-47 0.42089453033204194 1107 48-49 0.3693417488845512 1107 50-51 0.32163651627826084 1107 52-53 0.5971498507005393 1107 54-55 0.7631458612508624 1107 56-57 0.7060308992531716 1107 58-59 0.6887181405198106 1107 60-61 0.4332758103780421 1107 62-63 0.7750244590318474 1107 64-65 0.6876506903418615 1107 66-67 0.6515783091851901 1107 68-69 0.6431648790977675 1107 70-71 0.511471857449294 1107 72-73 0.5981703294273224 1107 74-75 0.5871618030971852 1107 76-77 0.6690563017204241 1107 78-79 0.7371005629934544 1107 80-81 0.7950546603852437 1107 82-83 0.7694544527547933 1107 84-85 0.9055791982991686 1107 86-87 1.0322859052530724 1107 88-89 0.9680826790156303 1107 90-91 0.6903320021035313 1107 92-93 0.7506803562196183 1107 94-95 0.8389074431048442 1107 96-97 0.8220242392176438 1107 98-99 0.6870644011712237 1107 100-101 0.7987767677627247 1108 1 0.006667705976472149 1108 2 0.0673853241513811 1108 3 0.0416276531373434 1108 4 -0.0888912612823276 1108 5 -0.08185514968467089 1108 6 -0.06627601319057419 1108 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-1.01603847491468 2316 62-63 -0.8239222551442609 2316 64-65 -0.8889020348115793 2316 66-67 -0.9400523934234002 2316 68-69 -0.677179527476838 2316 70-71 -0.9303150582730879 2316 72-73 -1.2630827321283924 2316 74-75 -1.0518977460057393 2316 76-77 -0.8149395080057324 2316 78-79 -0.829794060472075 2316 80-81 -0.7851167867346796 2316 82-83 -0.7857103548467137 2316 84-85 -0.9072527776215118 2316 86-87 -0.9005254881605254 2316 88-89 -0.8796159901297855 2316 90-91 -0.8519587792940158 2316 92-93 -0.9078291087827459 2316 94-95 -0.8116963842086591 2316 96-97 -0.9329915480816986 2316 98-99 -0.9513197613641857 2316 100-101 -1.3150065036017722 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 10.0 5 29.0 6 75.0 7 256.0 8 557.0 9 1077.0 10 1807.0 11 3255.0 12 5129.0 13 7109.0 14 7732.0 15 9328.0 16 11451.0 17 14337.0 18 13354.0 19 10268.0 20 9711.0 21 9913.0 22 10475.0 23 11480.0 24 13252.0 25 15772.0 26 19324.0 27 24278.0 28 30042.0 29 37710.0 30 46897.0 31 56998.0 32 70117.0 33 88371.0 34 114280.0 35 153562.0 36 222293.0 37 307900.0 38 268379.0 39 51862.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.14191711184184 18.58356001762493 13.37036753808114 19.904155332452085 2 14.306496897514856 25.401308940544396 36.209107741288896 24.08308642065185 3 26.862548392363067 24.59482111439442 29.22859280864754 19.31403768459498 4 11.751427578270357 15.890297442218948 41.93837617938621 30.419898800124486 5 11.086852205838193 40.915336555406654 33.700128789914714 14.297682448840435 6 26.219576188364503 38.60961489813585 17.823420109304603 17.347388804195045 7 26.732915923784034 33.10622711067136 22.585492419715596 17.575364545829007 8 23.52985068741541 28.807264649018666 19.56896933489685 28.093915328669073 9 21.830406188710285 22.464042862836617 25.676268380468336 30.029282567984755 10-11 25.334941741217808 24.056870180615267 28.359836473813427 22.248351604353502 12-13 31.274110193937105 22.213651730959505 26.060211560032297 20.45202651507109 14-15 23.226021127166423 26.02070251195251 30.667453692946918 20.08582266793416 16-17 19.773013083429536 28.31215447995385 30.480198929763695 21.434633506852922 18-19 18.484082646362793 33.342877820653825 28.502852123335 19.670187409648385 20-21 22.135584222540743 27.15376273140248 26.413456798549966 24.297196247506804 22-23 19.587441557708864 27.99084699843062 27.717737155738735 24.703974288121785 24-25 23.97467030570467 27.449905090993138 27.6207352369328 20.95468936636939 26-27 23.418461814731497 23.5591191580337 32.94130066030387 20.081118366930934 28-29 19.097987049528196 31.920137240417972 29.51621539222274 19.46566031783109 30-31 21.455507143544025 26.879637721892458 27.743803756317735 23.92105137824578 32-33 21.984252436649836 27.50233040519778 29.443005098832536 21.070412059319846 34-35 25.033526407828543 24.919471403589398 29.802299603689193 20.244702584892867 36-37 23.737422462664064 24.361452771927524 32.25838355853519 19.642741206873225 38-39 21.09264765053627 28.09412662895484 32.060726113466835 18.75249960704205 40-41 20.968498324056238 29.450927456660548 28.699467641393532 20.88110657788968 42-43 19.24743170294059 30.19577150536411 26.427135899431054 24.129660892264244 44-45 22.107801411555144 25.666448051361044 26.618880717948034 25.606869819135774 46-47 24.43830969202242 29.067058250050582 26.976938460600465 19.51769359732654 48-49 23.746082954760194 27.712008793958038 28.41562856689605 20.12627968438572 50-51 18.523084128222948 28.670988649865066 32.30270423736551 20.50322298454648 52-53 21.387687900650988 30.97422305480854 26.82980761472715 20.808281429813317 54-55 19.609525690129036 27.70895874831769 28.098304695052278 24.58321086650099 56-57 23.11631462555627 25.56152149710753 28.605547112231495 22.716616765104703 58-59 27.605637904423542 25.124858033140164 26.399071269790426 20.870432792645875 60-61 22.602432853256197 27.465985646523734 31.652148274158133 18.279433226061943 62-63 20.802138664341875 27.55474745930474 30.633127032184614 21.009986844168772 64-65 19.387570822443312 32.96059135279479 27.461306596112582 20.190531228649313 66-67 21.82427432291428 27.528654949113324 25.841747189574292 24.805323538398103 68-69 19.95529299833016 29.067317774067025 26.738130315327556 24.239258912275254 70-71 24.00935080977758 28.22240268850949 26.189821074451242 21.57842542726169 72-73 23.24237754941095 24.705020565147233 31.07596971645576 20.976632168986058 74-75 19.658927736212696 32.42512977586402 27.82493407319377 20.091008414729508 76-77 21.71362100448311 27.06353395089814 26.69232775826402 24.53051728635473 78-79 22.229532283475077 27.950974664722978 28.191236055527348 21.628256996274597 80-81 24.841854941815058 26.38586029243376 27.86813940120996 20.904145364541233 82-83 23.114469770548453 25.302094322464875 30.726749247304525 20.856686659682154 84-85 20.730995987948376 28.625398376676653 30.338323297896125 20.305282337478847 86-87 21.519131328690122 29.662818692436982 26.911441790656333 21.906608188216566 88-89 19.88769238888899 29.971233042084567 25.67975739177964 24.461317177246805 90-91 22.1035485818109 25.47292685960064 26.404767032373723 26.018757526214735 92-93 23.69538953666662 28.897421672414115 26.755604707192703 20.651584083726565 94-95 22.646313442682622 27.661699691365044 28.174079213777098 21.517907652175232 96-97 17.989462767362788 28.315058948503214 31.98467796951549 21.710800314618506 98-99 20.936605525403536 30.339910963566407 26.87518882931286 21.8482946817172 100-101 18.99539223626695 27.41197775897058 27.960965962003392 25.631664042759077 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 282.0 1 217.0 2 533.5 3 830.0 4 544.0 5 372.0 6 489.5 7 804.5 8 1156.5 9 1335.5 10 1315.0 11 1280.5 12 1362.0 13 1499.5 14 1688.5 15 1839.0 16 1974.0 17 2146.0 18 2345.0 19 2577.0 20 2834.5 21 3158.0 22 3674.0 23 4437.5 24 5383.0 25 6556.0 26 7971.0 27 9884.5 28 12500.0 29 15506.5 30 18475.0 31 21370.5 32 24041.0 33 26475.0 34 29236.5 35 32297.5 36 35149.5 37 38206.0 38 41048.0 39 42814.5 40 44365.5 41 45540.0 42 46271.0 43 47366.5 44 47921.5 45 49383.0 46 56000.0 47 101834.5 48 207798.0 49 208842.0 50 108030.0 51 58791.5 52 40887.0 53 33004.0 54 28533.5 55 24807.5 56 21119.5 57 17221.5 58 13778.0 59 11092.0 60 8861.5 61 6801.0 62 5024.5 63 3557.5 64 2321.5 65 1500.0 66 936.5 67 573.0 68 330.0 69 194.5 70 118.5 71 64.0 72 33.5 73 17.0 74 7.0 75 4.5 76 6.0 77 5.0 78 3.5 79 3.5 80 2.0 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04580140631549828 2 0.003943167431135614 3 0.041979567113013 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013346105151535925 16-17 0.0 18-19 0.0 20-21 0.011131864978667465 22-23 0.0 24-25 0.0 26-27 0.005399106174939533 28-29 0.01325510898004818 30-31 0.011192529092992627 32-33 0.006218071718329238 34-35 0.005672094689402767 36-37 0.02086845532785617 38-39 0.0401596436832581 40-41 0.005823754975215676 42-43 0.012041826693544914 44-45 0.010191571206627432 46-47 0.0042768200599240126 48-49 0.0010009578863651944 50-51 0.0016075990296168271 52-53 6.066411432516329E-4 54-55 9.402937720400311E-4 56-57 0.01067688412122874 58-59 0.00903895303444933 60-61 0.006642720518605381 62-63 0.006218071718329238 64-65 0.06548691141401378 66-67 0.029998404533793245 68-69 0.021657088814083295 70-71 0.01343710132302367 72-73 6.0664114325163296E-5 74-75 1.2132822865032659E-4 76-77 6.673052575767962E-4 78-79 4.8531291460130637E-4 80-81 8.189655433897045E-4 82-83 7.279693719019595E-4 84-85 7.583014290645411E-4 86-87 0.003912835373973033 88-89 1.2132822865032659E-4 90-91 9.706258292026127E-4 92-93 4.5498085743872463E-4 94-95 0.0020322478298929704 96-97 0.004580140631549829 98-99 0.006491060232792472 100-101 0.007037037261718941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1648421.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.12320816776924 #Duplication Level Percentage of deduplicated Percentage of total 1 82.42411739876411 41.31361194422904 2 11.467812976547451 11.496071541050666 3 2.606172006427796 3.918891059975797 4 1.1078495628244662 2.2211589702409147 5 0.5644988560115171 1.4147246835166432 6 0.3539892755332589 1.0645846888026815 7 0.2533169560137724 0.8887940963092771 8 0.18864539826664795 0.7564410053768744 9 0.1431528744770929 0.6457753194506881 >10 0.7990558696645361 7.261635331396699 >50 0.05139064732773913 1.8020924471989892 >100 0.03055080095385688 3.0022701970202745 >500 0.003067330255785812 1.0633579959173916 >1k 0.004048875937637271 4.88230821212664 >5k 0.0011042388920828922 3.6487858778422693 >10k+ 0.0012269321023143246 14.619496629545198 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 72303 4.386197458052282 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 34419 2.0879981509577954 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 30204 1.8322989090772321 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 19295 1.1705140859040257 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 16591 1.0064783207687842 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 15546 0.9430843212989886 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 13498 0.8188442151610541 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 12955 0.7859036010824905 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12724 0.7718901906733777 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 10189 0.6181066608590887 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 9851 0.5976021902171836 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9358 0.5676947818548781 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7593 0.46062262007096494 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6068 0.36810984572509087 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5685 0.3448754899385533 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5338 0.32382504226772163 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5292 0.32103449300876413 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5102 0.30950831128698314 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5045 0.3060504567704488 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4826 0.29276501573323804 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4326 0.2624329585706564 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4287 0.260067058111975 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4251 0.25788314999626916 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4246 0.25757982942464336 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3990 0.24204981615740156 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3734 0.22651980289015972 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3532 0.21426565179647675 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3065 0.1859355104066255 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2952 0.17908046548788203 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2885 0.1750159698280961 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2638 0.16003193358978077 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2467 0.14965837004017785 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2372 0.14389527917928732 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2324 0.1409834016916795 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1997 0.1211462363073511 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1994 0.1209642439643756 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1800 0.10919540578529392 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1703 0.10331098669575309 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1692 0.10264368143817629 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1690 0.10252235320952596 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.099617148774494E-5 0.0 12-13 0.0 6.066411432516329E-5 0.0 1.8199234297548988E-4 0.0 14-15 0.0 6.066411432516329E-5 0.0 4.24648800276143E-4 0.0 16-17 0.0 6.066411432516329E-5 0.0 5.15644971763888E-4 0.0 18-19 0.0 6.066411432516329E-5 0.0 7.583014290645411E-4 0.0 20-21 0.0 6.066411432516329E-5 0.0 0.0014256066866413374 0.0 22-23 0.0 6.066411432516329E-5 0.0 0.0021839081157058785 0.0 24-25 0.0 6.066411432516329E-5 0.0 0.0030635377734207466 0.0 26-27 0.0 6.066411432516329E-5 0.0 0.004064495659785941 0.0 28-29 0.0 6.066411432516329E-5 0.0 0.007097701376044105 0.0 30-31 0.0 6.066411432516329E-5 0.0 0.013922414237624975 0.0 32-33 0.0 6.066411432516329E-5 0.0 0.028542465789989327 0.0 34-35 0.0 6.066411432516329E-5 0.0 0.04261654031342721 0.0 36-37 0.0 6.066411432516329E-5 0.0 0.04965357757514616 0.0 38-39 0.0 6.066411432516329E-5 0.0 0.05696360335132833 0.0 40-41 0.0 6.066411432516329E-5 0.0 0.07103767787476623 0.0 42-43 0.0 6.066411432516329E-5 0.0 0.08823595428595002 0.0 44-45 0.0 6.066411432516329E-5 0.0 0.10440294075360602 0.0 46-47 0.0 6.066411432516329E-5 0.0 0.12072158750707496 0.0 48-49 0.0 6.066411432516329E-5 0.0 0.1307311663707269 0.0 50-51 0.0 6.066411432516329E-5 0.0 0.14883940449678815 0.0 52-53 0.0 6.066411432516329E-5 0.0 0.23037197414980762 0.0 54-55 0.0 6.066411432516329E-5 0.0 0.32376437815339654 0.0 56-57 0.0 6.066411432516329E-5 0.0 0.37745211933116596 0.0 58-59 0.0 6.066411432516329E-5 0.0 0.49201629923423695 0.0 60-61 0.0 1.2132822865032658E-4 0.0 0.6308157928102105 0.0 62-63 0.0 1.2132822865032658E-4 0.0 0.6760105579824571 0.0 64-65 0.0 1.2132822865032658E-4 0.0 0.7151692437793501 0.0 66-67 0.0 1.8199234297548988E-4 0.0 0.973932023433334 0.0 68-69 0.0 1.8199234297548988E-4 0.0 1.164478006528672 0.0 70-71 0.0 1.8199234297548988E-4 0.0 1.2034546999825895 0.0 72-73 0.0 2.4265645730065316E-4 0.0 1.2852299260929096 0.0 74-75 0.0 2.4265645730065316E-4 0.0 1.4130795470331912 0.0 76-77 0.0 2.4265645730065316E-4 0.0 1.5221536245898348 0.0 78-79 0.0 2.4265645730065316E-4 0.0 1.623129042884069 0.0 80-81 0.0 2.4265645730065316E-4 0.0 1.8076389466040532 0.0 82-83 0.0 2.4265645730065316E-4 0.0 2.0070722224480275 0.0 84-85 0.0 2.4265645730065316E-4 0.0 2.214573825497249 0.0 86-87 0.0 2.729885144632348E-4 0.0 2.686388974661206 0.0 88-89 0.0 3.0332057162581644E-4 0.0 3.3710745009921617 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2530 0.0 56.32796 2 TATAACG 285 0.0 55.00376 2 ACCTGGG 2655 0.0 52.243042 3 GTACCTG 2840 0.0 51.037495 1 ATAACGC 340 0.0 48.898914 3 TAACGCA 325 0.0 48.216507 4 TACGCAG 1370 0.0 42.633373 4 GTACGCA 1410 0.0 42.448418 3 AGTACGC 1390 0.0 42.377 2 ACGACGG 45 9.6403126E-4 42.20974 6 CCTGGGG 1740 0.0 40.390354 4 GAGTACG 1520 0.0 40.33239 1 GTACACG 245 0.0 38.79464 1 GTACCCG 190 0.0 37.5185 1 ACGCAGT 1570 0.0 37.504833 5 CGCAGTG 1635 0.0 36.30425 6 GTATAAC 535 0.0 35.531544 1 GTACCGG 115 1.0323856E-7 33.05978 1 TACACGG 220 0.0 32.388577 2 TACCCGG 235 0.0 30.321222 2 >>END_MODULE