##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003785_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2126643 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.735700350270356 31.0 31.0 34.0 30.0 34.0 2 31.912513759949366 31.0 31.0 34.0 30.0 34.0 3 31.983719411297525 33.0 31.0 34.0 30.0 34.0 4 35.69220315774674 37.0 35.0 37.0 33.0 37.0 5 35.567935003665404 37.0 35.0 37.0 33.0 37.0 6 35.6458705104712 37.0 35.0 37.0 33.0 37.0 7 35.59895431438187 37.0 35.0 37.0 33.0 37.0 8 35.60798591959252 37.0 35.0 37.0 33.0 37.0 9 37.18834472922818 39.0 37.0 39.0 33.0 39.0 10-11 37.094053632885256 39.0 37.0 39.0 33.0 39.0 12-13 37.068344569351794 39.0 37.0 39.0 33.0 39.0 14-15 38.138654207593845 40.0 37.0 41.0 33.0 41.0 16-17 38.19447058109894 40.0 37.5 41.0 33.0 41.0 18-19 38.25146204605099 40.0 38.0 41.0 33.0 41.0 20-21 38.18509359586917 40.0 37.5 41.0 33.0 41.0 22-23 38.10254095304195 40.0 37.0 41.0 33.0 41.0 24-25 37.93952910761233 40.0 37.0 41.0 33.0 41.0 26-27 37.728063431426904 39.5 37.0 41.0 32.5 41.0 28-29 37.611355079343355 39.0 37.0 41.0 32.0 41.0 30-31 37.441073325424156 39.0 36.5 41.0 32.0 41.0 32-33 37.18124927409067 39.0 36.0 41.0 31.0 41.0 34-35 37.10523463505628 39.0 36.0 41.0 31.0 41.0 36-37 36.93414527026868 39.0 35.5 40.5 30.5 41.0 38-39 36.71197728062491 39.0 35.0 40.0 30.0 41.0 40-41 36.83577191846493 39.0 36.0 41.0 30.5 41.0 42-43 36.714077068882744 39.0 35.0 41.0 30.0 41.0 44-45 36.44726477363619 39.0 35.0 41.0 30.0 41.0 46-47 36.28982673631634 39.0 35.0 41.0 30.0 41.0 48-49 36.01887599376106 39.0 35.0 41.0 29.0 41.0 50-51 35.77071445465929 39.0 35.0 40.0 28.0 41.0 52-53 35.62590923817491 39.0 35.0 40.0 28.0 41.0 54-55 35.45615131453657 38.5 34.5 40.0 27.5 41.0 56-57 35.13444499147248 38.0 34.0 40.0 26.5 41.0 58-59 34.85139278195729 38.0 34.0 40.0 26.0 41.0 60-61 34.392833446892595 38.0 33.5 40.0 25.0 41.0 62-63 34.21249687888376 37.0 33.0 40.0 25.5 41.0 64-65 33.76500240049693 37.0 33.0 39.5 24.0 41.0 66-67 33.342105844751565 36.0 32.0 39.0 22.5 40.5 68-69 32.977829612210414 36.0 32.0 39.0 22.0 40.0 70-71 32.44791532946527 35.0 31.5 38.5 21.5 40.0 72-73 32.017786718316145 35.0 31.0 37.0 21.0 39.5 74-75 31.66694151298549 35.0 31.0 37.0 20.0 39.0 76-77 30.639270907246775 34.0 29.5 36.0 20.0 38.5 78-79 31.061402172343925 34.0 31.0 36.0 20.0 38.5 80-81 30.80246825630818 34.0 31.0 36.0 18.0 37.5 82-83 30.47330393488705 34.0 30.5 35.0 16.5 37.0 84-85 30.14432417664836 34.0 30.0 35.0 14.0 36.5 86-87 29.956312836710254 34.0 30.0 35.0 10.5 36.0 88-89 29.635739990209924 34.0 30.0 35.0 7.0 36.0 90-91 29.293107493829478 34.0 29.0 35.0 3.5 35.5 92-93 29.06503113122419 34.0 29.0 35.0 2.0 35.0 94-95 28.695992698351347 34.0 29.0 35.0 2.0 35.0 96-97 28.333981773151393 34.0 29.0 35.0 2.0 35.0 98-99 28.019354917586075 34.0 29.0 35.0 2.0 35.0 100-101 27.064617803740447 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1413228689386905 1101 2 0.23376880433850644 1101 3 0.1862632490839502 1101 4 0.08391211653832897 1101 5 0.20781168551079787 1101 6 0.15750736986639424 1101 7 0.20513041268424104 1101 8 0.2218743414255684 1101 9 0.2685704082953606 1101 10-11 0.27261869186746424 1101 12-13 0.3762794273540422 1101 14-15 0.49099486881350174 1101 16-17 0.37942420390517384 1101 18-19 0.38478483720859913 1101 20-21 0.293334573259564 1101 22-23 0.27890427678377705 1101 24-25 0.42161382940650327 1101 26-27 0.37746381293781894 1101 28-29 0.4003778039230639 1101 30-31 0.38597122097377223 1101 32-33 0.4139697498202466 1101 34-35 0.5406572363066644 1101 36-37 0.5092616659276885 1101 38-39 0.4317089069849871 1101 40-41 0.4798751196280051 1101 42-43 0.5546569039181932 1101 44-45 0.6622719345397812 1101 46-47 0.8333547308025473 1101 48-49 0.7043450056136962 1101 50-51 0.6506854052375601 1101 52-53 0.517516880655208 1101 54-55 0.6861048119268887 1101 56-57 0.7594921757075781 1101 58-59 0.7422915728092647 1101 60-61 0.6004152926180026 1101 62-63 0.49882161610381104 1101 64-65 0.46883092498511303 1101 66-67 0.3718488763357044 1101 68-69 0.6206239886437999 1101 70-71 0.7090454733529512 1101 72-73 0.6289932504608231 1101 74-75 0.553919879150552 1101 76-77 0.6337682922176349 1101 78-79 0.704556430053394 1101 80-81 0.5797053134215773 1101 82-83 0.642940701835812 1101 84-85 0.5792424052929661 1101 86-87 0.37332492385967697 1101 88-89 0.45191063156582345 1101 90-91 0.49986071780779184 1101 92-93 0.6152936728107612 1101 94-95 0.5391235454710426 1101 96-97 0.5007481594232424 1101 98-99 0.5321919450991501 1101 100-101 0.6216825856997197 1102 1 0.18034484219958813 1102 2 0.26648456541347443 1102 3 0.2527711568152995 1102 4 0.14974432575597518 1102 5 0.2670016769378165 1102 6 0.08896330197082847 1102 7 0.19076283146368667 1102 8 0.15870728503131915 1102 9 0.15833014969241788 1102 10-11 0.2961051302695097 1102 12-13 0.2257730375629663 1102 14-15 0.2751240965383417 1102 16-17 0.2757514183618639 1102 18-19 0.1941967582634092 1102 20-21 0.2471500113881291 1102 22-23 0.26297896313245417 1102 24-25 0.3246643823485158 1102 26-27 0.1650420529260188 1102 28-29 0.26077177702418197 1102 30-31 0.3081591905289258 1102 32-33 0.27313177918962595 1102 34-35 0.35327503799729953 1102 36-37 0.3340547159736218 1102 38-39 0.2758840416484176 1102 40-41 0.351351900365529 1102 42-43 0.4953526060577431 1102 44-45 0.6771366872906626 1102 46-47 0.49533677977608903 1102 48-49 0.40458283674130513 1102 50-51 0.593461745649492 1102 52-53 0.500707612615841 1102 54-55 0.4688979295734015 1102 56-57 0.5251950219992239 1102 58-59 0.5269895672229978 1102 60-61 0.48612598979506316 1102 62-63 0.3449109994328907 1102 64-65 0.22822771272851128 1102 66-67 0.3458864899123597 1102 68-69 0.40095944115285675 1102 70-71 0.34835897411038985 1102 72-73 0.5332070710919083 1102 74-75 0.43969525779061414 1102 76-77 0.29464138442174814 1102 78-79 0.43796609570707545 1102 80-81 0.5048035647175091 1102 82-83 0.5171529246350275 1102 84-85 0.43077809071674267 1102 86-87 0.47746215610760956 1102 88-89 0.29275582316737214 1102 90-91 0.39816056324828963 1102 92-93 0.3943105299369343 1102 94-95 0.38165990382785964 1102 96-97 0.5263381281118846 1102 98-99 0.4680953807449342 1102 100-101 0.6739740916869792 1103 1 -0.03613437033806832 1103 2 0.05822342238245426 1103 3 0.10921688947718877 1103 4 0.02808201402928745 1103 5 0.10009046468731242 1103 6 0.048808498334707906 1103 7 0.06381977091030677 1103 8 0.16329885746785777 1103 9 0.17854060627993817 1103 10-11 0.09814989899813042 1103 12-13 0.009628542204374924 1103 14-15 0.09072919389837608 1103 16-17 0.04731367388085772 1103 18-19 -0.08000840060692127 1103 20-21 -0.017755753040482603 1103 22-23 0.05343687222283933 1103 24-25 0.07033414710016928 1103 26-27 4.881371918656896E-4 1103 28-29 0.10353864728395479 1103 30-31 0.07043175946492397 1103 32-33 0.2792862353007166 1103 34-35 0.2166500607373436 1103 36-37 0.08091406052184169 1103 38-39 0.11026984826950326 1103 40-41 0.02040822307758816 1103 42-43 0.1755629321194263 1103 44-45 0.1922274312960539 1103 46-47 0.21989619391089832 1103 48-49 0.13043771975345209 1103 50-51 0.2743410165974538 1103 52-53 0.30035356129797464 1103 54-55 0.38605269486756555 1103 56-57 0.293992495671759 1103 58-59 0.31085967444180795 1103 60-61 0.3768691794058654 1103 62-63 0.31478743225981276 1103 64-65 0.35049444063353974 1103 66-67 0.17084041837278363 1103 68-69 0.27805476824651265 1103 70-71 0.188985954941856 1103 72-73 0.34586252939665485 1103 74-75 0.21750235855900613 1103 76-77 0.23681419899104128 1103 78-79 0.17254043057598878 1103 80-81 0.36706422317138276 1103 82-83 0.48575330430204033 1103 84-85 0.31679559259917056 1103 86-87 0.4515238997206126 1103 88-89 0.3191518173296046 1103 90-91 0.39233372697417934 1103 92-93 0.4597399476856765 1103 94-95 0.3487975783959598 1103 96-97 0.5139896502470265 1103 98-99 0.4949766862643017 1103 100-101 0.7900598504214003 1104 1 0.005378560321855019 1104 2 -0.056224188280687315 1104 3 0.046442942924180386 1104 4 0.010232453763727278 1104 5 -0.08414004298714417 1104 6 -9.207670094042442E-4 1104 7 -0.06369615848390708 1104 8 -0.1998561293436012 1104 9 0.0044616491334039665 1104 10-11 -0.09543069415402528 1104 12-13 -0.03807503850611482 1104 14-15 -0.05546649995727648 1104 16-17 -0.04485004537951909 1104 18-19 -0.10693000786999818 1104 20-21 -0.1741984828103753 1104 22-23 -0.1388706107874782 1104 24-25 -0.12125825835101267 1104 26-27 -0.07168223458554479 1104 28-29 -0.06388031045096909 1104 30-31 -0.06020797901905439 1104 32-33 -0.07869732080634151 1104 34-35 0.0646704862773575 1104 36-37 0.0050240100310716684 1104 38-39 0.11460282715336945 1104 40-41 0.22491606860928215 1104 42-43 -0.022756559092258044 1104 44-45 0.23815853795213116 1104 46-47 0.250724964550578 1104 48-49 0.1219612022712866 1104 50-51 0.2575775069457791 1104 52-53 0.22403420919054895 1104 54-55 0.22415964564250856 1104 56-57 0.27885342796624 1104 58-59 0.2509672098628002 1104 60-61 0.14403984112554014 1104 62-63 0.11020510302518005 1104 64-65 0.25568757024318245 1104 66-67 0.17465107603837282 1104 68-69 0.20143705619199892 1104 70-71 0.25997028428498226 1104 72-73 0.25995969253688145 1104 74-75 0.23295517610913308 1104 76-77 0.1926546310917523 1104 78-79 0.36241954080523797 1104 80-81 0.35704931112591964 1104 82-83 0.36927269667827645 1104 84-85 0.37938401011127354 1104 86-87 0.4173041649744249 1104 88-89 0.4459184882873011 1104 90-91 0.4365774716610531 1104 92-93 0.5254168880584125 1104 94-95 0.5271553521903165 1104 96-97 0.5765123418431699 1104 98-99 0.4896824764879284 1104 100-101 0.527119454899335 1105 1 -0.10051114664764427 1105 2 -0.14511385810516586 1105 3 -0.012382385900284731 1105 4 -0.06569415059156114 1105 5 -0.13380420831307305 1105 6 0.014254612106498143 1105 7 0.013145233012217261 1105 8 0.08279998716648862 1105 9 0.01567343619484518 1105 10-11 -0.08238805822692541 1105 12-13 0.03422382667713464 1105 14-15 0.06343343208919094 1105 16-17 0.17557305095645148 1105 18-19 -0.026677556480812825 1105 20-21 -0.08798871687498178 1105 22-23 0.141317455901671 1105 24-25 0.09825578647209454 1105 26-27 0.02210529736098721 1105 28-29 0.10681388990052199 1105 30-31 0.16690722953566706 1105 32-33 0.13735950597478563 1105 34-35 0.16879562274889537 1105 36-37 0.04662961805498611 1105 38-39 0.006933072594549117 1105 40-41 0.2375905318455409 1105 42-43 0.2357611215727431 1105 44-45 0.32482323933167123 1105 46-47 0.15100269640286967 1105 48-49 0.19892285987141634 1105 50-51 0.11051181780006658 1105 52-53 0.36338383996177726 1105 54-55 0.24638301903662096 1105 56-57 0.08960574570619428 1105 58-59 0.21278367777934193 1105 60-61 0.1571476629197619 1105 62-63 0.19802002541758412 1105 64-65 -0.026149626050766983 1105 66-67 0.01848822571896136 1105 68-69 -0.050746579314854046 1105 70-71 0.09259819607211028 1105 72-73 0.11434065622434986 1105 74-75 0.11991359626591702 1105 76-77 0.14897400032562302 1105 78-79 0.2033731262828482 1105 80-81 0.04222123794003707 1105 82-83 0.09490128508742757 1105 84-85 0.07582389672428391 1105 86-87 0.1304264441928069 1105 88-89 0.2801023940059615 1105 90-91 0.3570357829319448 1105 92-93 0.37836603871205554 1105 94-95 0.31407376861803016 1105 96-97 0.2839832066739021 1105 98-99 0.2033418107763474 1105 100-101 0.44035087492428815 1106 1 -0.187153664603386 1106 2 -0.1751005191914956 1106 3 -0.15227761142813634 1106 4 -0.1075761500475636 1106 5 -0.022411437094810083 1106 6 -0.12350525039489924 1106 7 -0.10379678734478404 1106 8 -0.1034824895935671 1106 9 -0.2690393182150359 1106 10-11 -0.05171613571388889 1106 12-13 -0.18491025129993233 1106 14-15 -0.1415734184198172 1106 16-17 -0.146881923894135 1106 18-19 -0.3172836644232717 1106 20-21 -0.11272275965213652 1106 22-23 -0.1972076888769152 1106 24-25 -0.16318218463197098 1106 26-27 -0.05989533758476995 1106 28-29 -0.05501591179260856 1106 30-31 -0.03459920876386491 1106 32-33 0.011557192212208633 1106 34-35 0.08008342581128858 1106 36-37 0.1233804767896558 1106 38-39 0.11465048467687922 1106 40-41 0.19398474727068304 1106 42-43 0.2370002763369783 1106 44-45 0.3892629712733964 1106 46-47 0.44295237989840786 1106 48-49 0.43831899192328905 1106 50-51 0.35897961114458354 1106 52-53 0.2865277183351367 1106 54-55 0.3614405178387301 1106 56-57 0.23985378718482764 1106 58-59 0.4885632968624165 1106 60-61 0.46709003780218694 1106 62-63 0.40516823893788256 1106 64-65 0.21558902639895194 1106 66-67 0.16603469507525404 1106 68-69 0.19017965413466698 1106 70-71 0.31320363875310164 1106 72-73 0.41771125823545674 1106 74-75 0.37408032258155544 1106 76-77 0.1930918888699047 1106 78-79 0.3752916393308112 1106 80-81 0.3948733996608915 1106 82-83 0.46910865551091163 1106 84-85 0.3043983719045542 1106 86-87 0.3291537316186073 1106 88-89 0.2154701362348206 1106 90-91 0.3151706255386557 1106 92-93 0.35998471672802523 1106 94-95 0.37018110055740294 1106 96-97 0.27166692199925535 1106 98-99 0.029152040308396465 1106 100-101 0.11160770543335019 1107 1 -0.22822201349963578 1107 2 -0.1440010510578844 1107 3 -0.045154338177606945 1107 4 0.02513048225688408 1107 5 -0.026456962663466754 1107 6 -0.06564837812907598 1107 7 -0.023050113455759913 1107 8 0.09492371517659848 1107 9 -0.09999980866983549 1107 10-11 -0.10184076834601541 1107 12-13 -0.18067901853777357 1107 14-15 -0.05018459796816188 1107 16-17 -0.07303928250770042 1107 18-19 -0.0768924363266521 1107 20-21 -0.07947487057332836 1107 22-23 -0.0545634359029421 1107 24-25 -0.12840687550919228 1107 26-27 -0.12440999189633573 1107 28-29 -0.08657241978567498 1107 30-31 -0.012716278677494586 1107 32-33 -0.1593533512859011 1107 34-35 -0.21338052116961848 1107 36-37 -0.22197188870978124 1107 38-39 -0.11512923916182416 1107 40-41 0.009207448869240409 1107 42-43 -0.021088612540928864 1107 44-45 0.1262186780690442 1107 46-47 0.051556244832070774 1107 48-49 0.09515297957691615 1107 50-51 0.19274238978878344 1107 52-53 0.21427007487169192 1107 54-55 0.2026104558597197 1107 56-57 0.08421460229978095 1107 58-59 0.020799989885574632 1107 60-61 -0.05187334785617992 1107 62-63 0.23407001132338934 1107 64-65 0.08104818033032757 1107 66-67 0.262343088126066 1107 68-69 0.13572383057701387 1107 70-71 0.048020314987248014 1107 72-73 0.18514382601364332 1107 74-75 0.1567590432060264 1107 76-77 0.017405470354834307 1107 78-79 0.11133598467650785 1107 80-81 0.19709560925277003 1107 82-83 0.18629229163057914 1107 84-85 0.20167770591877598 1107 86-87 0.3628191220991823 1107 88-89 0.1904388328965183 1107 90-91 -0.07625924077440516 1107 92-93 -0.12100889954086469 1107 94-95 -0.12041121766392138 1107 96-97 -0.09412250161157942 1107 98-99 0.024783411405735478 1107 100-101 0.07739359529452372 1108 1 -0.050191384882364076 1108 2 -0.10747675923124689 1108 3 -0.09848312306099416 1108 4 -0.06560363278904902 1108 5 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-1.0657625862200675 2316 96-97 -1.3664116438832075 2316 98-99 -1.4392303958543096 2316 100-101 -1.4472133522491184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 16.0 5 36.0 6 95.0 7 248.0 8 633.0 9 1385.0 10 2246.0 11 3677.0 12 5333.0 13 7563.0 14 9098.0 15 11712.0 16 14358.0 17 18669.0 18 17099.0 19 12712.0 20 11637.0 21 11778.0 22 12732.0 23 13350.0 24 15787.0 25 19043.0 26 24000.0 27 29643.0 28 37492.0 29 47271.0 30 57837.0 31 71224.0 32 88509.0 33 112101.0 34 145358.0 35 196097.0 36 287332.0 37 402198.0 38 364854.0 39 73456.0 40 58.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.13854091291039 19.29835621929497 14.774642360781117 19.788460507013518 2 14.613453364375628 24.52584242822096 36.35531734100268 24.505386866400727 3 25.2645116254347 25.097523447727294 29.385685773406923 20.25227915343109 4 11.640176559958583 16.11497557417959 42.040765657423464 30.204082208438372 5 11.74503666106629 40.04160547868166 34.627767801177725 13.585590059074326 6 27.484067612664653 39.06217451636218 16.592018500519362 16.86173937045381 7 26.166357023722362 33.418208886023656 21.761668507596244 18.653765582657737 8 24.28282509100023 29.538573234905908 20.45002381687947 25.728577857214397 9 22.18294278823479 21.766605866617013 24.67409903777926 31.376352307368936 10-11 25.577588716112672 24.42709001934034 27.99096980546335 22.004351459083633 12-13 29.816083333235373 23.29613463475878 26.165681349827253 20.7221006821786 14-15 22.804881288792657 25.967919372165237 30.358568247452933 20.868631091589172 16-17 19.779977175294583 28.96022040370669 29.519787759393562 21.740014661605166 18-19 18.851401010888992 32.521655021552746 28.887076956499047 19.739867011059214 20-21 22.18412483131872 26.54177452588774 27.09086603949405 24.183234603299486 22-23 20.08230812599952 27.756069072242028 28.345683784255275 23.815939017503172 24-25 23.866088478414103 27.38524989854903 27.95560891038129 20.79305271265558 26-27 22.58370557494812 24.374768108247657 32.61240960042435 20.42911671637987 28-29 19.612001413657218 31.18115857344775 29.509953327208738 19.696886685686295 30-31 21.478687035917535 26.951424222779806 28.199542851069666 23.370345890232997 32-33 21.66066509838222 27.47427437453783 29.502651623362503 21.362408903717444 34-35 24.436626772084548 25.649499816836986 29.441420987613103 20.472452423465366 36-37 23.116074909734984 24.923021353667203 32.264881793627154 19.696021942970663 38-39 21.178541858758443 27.809730350187227 31.26216058558982 19.7495672054645 40-41 20.705879420827976 28.846001016668758 29.428492964906116 21.019626597597142 42-43 19.99646352884799 29.610021757292266 26.572700935166182 23.820813778693562 44-45 21.931821976050976 25.965031581056163 27.61556559741125 24.487580845481613 46-47 24.15714538974575 28.42496754136859 27.76730699869695 19.650580070188706 48-49 23.29478941127982 27.834948532214128 28.132486199354233 20.737775857151824 50-51 18.902711128770974 28.315930791096605 32.1413943821255 20.639963698006916 52-53 21.494531010844874 30.741004416853546 26.290882228054098 21.473582344247486 54-55 19.941640004739895 27.510124008524294 28.495909956983546 24.052326029752265 56-57 23.387941368314078 25.710993135433284 28.31721944839865 22.58384604785398 58-59 26.135713288091182 26.142978805849847 26.65556225937381 21.065745646685162 60-61 22.04537070280786 27.3034685077721 31.565351084162906 19.085809705257134 62-63 20.887833129637528 27.74275735620871 29.85541751683606 21.513991997317707 64-65 19.833924158808994 31.728692753340837 27.871150634378562 20.56623245347161 66-67 21.82570584230103 26.926703411585823 26.493902044646607 24.753688701466544 68-69 20.352342533892703 28.74892790009052 27.41024639425197 23.48848317176481 70-71 23.697568901884843 28.011301308256943 26.585657496993022 21.705472292865196 72-73 22.31806457179763 25.38583742762352 30.80866277116419 21.487435229414658 74-75 20.352862289144785 31.59624797273818 27.546661701080154 20.50422803703688 76-77 21.79938898719243 27.02885265111564 27.11657347553621 24.05518488615572 78-79 21.83038720380395 27.77716125780107 28.446200376934044 21.94625116146094 80-81 24.017505647438103 26.973828991248116 27.6998845120068 21.308780849306977 82-83 22.491741039047938 25.809291181186005 30.81176105193688 20.887206727829177 84-85 20.81254042494063 28.225102057178354 29.334159054258002 21.62819846362302 86-87 21.285371813151055 28.829320891283167 27.603557307792144 22.281749987773637 88-89 20.57240529661814 28.949563630891923 25.93210416431554 24.545926908174398 90-91 21.859796955746884 25.30223875333274 27.661017666448796 25.17694662447158 92-93 23.219477513087565 27.957662732285765 28.069788120868772 20.753071633757898 94-95 21.797998863909957 27.658431894787526 28.44711162272782 22.096457618574696 96-97 17.764837968269642 28.04968228496188 32.097060327649146 22.088419419119333 98-99 20.72194339713588 30.179063312300947 26.3122786103342 22.786714680228968 100-101 18.96446886705276 27.302775289273885 28.472285904408533 25.26046993926483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 130.0 1 109.5 2 190.5 3 284.5 4 237.0 5 208.0 6 294.5 7 548.0 8 867.5 9 1046.0 10 1067.0 11 1127.0 12 1261.5 13 1442.5 14 1643.0 15 1867.5 16 2060.5 17 2282.0 18 2571.0 19 2823.5 20 3118.5 21 3526.0 22 4118.0 23 4868.5 24 5855.0 25 7276.0 26 9302.0 27 11918.0 28 15848.5 29 20318.5 30 24626.5 31 29659.0 32 34206.5 33 37937.0 34 41586.5 35 45941.5 36 49917.5 37 53404.0 38 56846.0 39 58299.0 40 59463.0 41 60320.5 42 60782.5 43 63188.0 44 64428.0 45 65870.0 46 75494.0 47 136389.5 48 260014.0 49 252511.0 50 132504.0 51 74846.0 52 52434.0 53 42388.0 54 36880.5 55 32032.0 56 26828.0 57 21669.5 58 17298.5 59 13896.0 60 11000.0 61 8275.0 62 6072.5 63 4265.0 64 2739.5 65 1775.5 66 1124.0 67 673.0 68 387.0 69 232.0 70 123.0 71 61.0 72 35.0 73 23.5 74 15.5 75 9.5 76 9.0 77 5.0 78 2.5 79 2.5 80 4.0 81 2.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0553454434994496 2 0.0038558422828843396 3 0.034984715347145715 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.3511233432221583E-5 14-15 0.0015282301730944025 16-17 0.0 18-19 0.0 20-21 0.01041547641047416 22-23 0.0 24-25 0.0 26-27 0.0052665162888176345 28-29 0.01144997068149191 30-31 0.00952204954004974 32-33 0.005431094922843186 34-35 0.005736740957462066 36-37 0.018997076613235037 38-39 0.043401736915881034 40-41 0.004232022017799885 42-43 0.010791656145389706 44-45 0.009874718041533065 46-47 0.004232022017799885 48-49 0.001010983037585528 50-51 0.0017868537408488403 52-53 7.523594698310907E-4 54-55 7.053370029666474E-4 56-57 0.010156852842719723 58-59 0.007523594698310905 60-61 0.004443623118689879 62-63 0.004984381487630975 64-65 0.07758707032633122 66-67 0.034114799710153514 68-69 0.026262047743791508 70-71 0.016011149967342896 72-73 1.175561671611079E-4 74-75 2.82134801186659E-4 76-77 4.702246686444316E-4 78-79 5.172471355088747E-4 80-81 7.053370029666474E-4 82-83 6.348033026699827E-4 84-85 7.758707032633122E-4 86-87 0.004279044484664328 88-89 1.410674005933295E-4 90-91 6.112920692377611E-4 92-93 2.82134801186659E-4 94-95 0.0023981458100866015 96-97 0.006136431925809833 98-99 0.007547105931743128 100-101 0.009310448439159746 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2126643.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.715737542603414 #Duplication Level Percentage of deduplicated Percentage of total 1 82.16521574370795 39.20573869558155 2 11.096237239307746 10.589302876425414 3 2.7959558468142616 4.002332861018904 4 1.113721480769072 2.1256816748774674 5 0.6192752262992889 1.4774587082366601 6 0.3821675702006648 1.0941244486193629 7 0.27717585497399727 0.9257955244360171 8 0.20493137002364775 0.7822761173035616 9 0.16172878090122295 0.6945307256311168 >10 1.0423645162640307 9.471034496376319 >50 0.0874537536970357 2.864029346849609 >100 0.044860167820457965 3.9820446962907394 >500 0.003036109075172807 1.0415698890728011 >1k 0.0036237430897223828 3.6480181568916454 >5k 0.0010773290266742218 3.7011528017173716 >10k+ 0.0011752680290991512 14.394908980671476 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 81303 3.823067623479823 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 33090 1.5559734285444242 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 30954 1.4555334393219737 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 25145 1.1823799293064234 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 22425 1.054478819435138 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 22311 1.0491182582125913 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 20581 0.9677693905371046 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 16453 0.7736606473206833 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 15691 0.7378295275699777 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 14908 0.7010109360151187 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12942 0.6085647661596234 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 12226 0.5748966798846821 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8900 0.41849995509354415 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8438 0.3967755754021714 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7830 0.36818591554859 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 7727 0.36334260146155234 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 7158 0.33658681781568417 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 6986 0.3284989535149999 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6973 0.32788766144576215 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6690 0.31458030332312475 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6503 0.3057871020194739 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 6434 0.3025425518058273 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5560 0.26144491576630396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4552 0.21404626916694527 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4345 0.20431261852600552 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4092 0.1924159344093014 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3836 0.18037818289200397 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3675 0.17280756572682862 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3565 0.16763509437173987 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3302 0.15526818558639133 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3165 0.1488261076259626 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2893 0.13603599663883406 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2582 0.12141200944399225 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2296 0.10796358392076151 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2268 0.1066469548485571 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.404493372888632E-5 0.0 10-11 0.0 0.0 0.0 1.175561671611079E-4 0.0 12-13 0.0 0.0 0.0 1.6457863402555105E-4 0.0 14-15 0.0 0.0 0.0 3.9969096834776684E-4 0.0 16-17 0.0 0.0 0.0 4.7022466864443164E-4 0.0 18-19 0.0 0.0 0.0 7.28848236398869E-4 0.0 20-21 0.0 0.0 0.0 0.001058005504449971 0.0 22-23 0.0 0.0 0.0 0.0019044099080099482 0.0 24-25 0.0 0.0 0.0 0.0031975277467821347 0.0 26-27 0.0 0.0 0.0 0.004490645585554322 0.0 28-29 0.0 0.0 0.0 0.007100392496530918 0.0 30-31 0.0 0.0 0.0 0.012554998652806324 0.0 32-33 0.0 0.0 0.0 0.02198300325912718 0.0 34-35 0.0 0.0 0.0 0.0309407831968036 0.0 36-37 0.0 0.0 0.0 0.040298254102827794 0.0 38-39 0.0 0.0 0.0 0.05170120231745526 0.0 40-41 0.0 0.0 0.0 0.06517313907411823 0.0 42-43 0.0 0.0 0.0 0.08576897956074434 0.0 44-45 0.0 0.0 0.0 0.10159203966062945 0.0 46-47 0.0 0.0 0.0 0.13154535105327975 0.0 48-49 0.0 0.0 0.0 0.16629495406610323 0.0 50-51 0.0 0.0 0.0 0.17852079545085847 0.0 52-53 0.0 0.0 0.0 0.1996103718395612 0.0 54-55 0.0 0.0 0.0 0.2478554228424799 0.0 56-57 0.0 0.0 0.0 0.32318541475931784 0.0 58-59 0.0 0.0 0.0 0.4161958542171864 0.0 60-61 0.0 0.0 0.0 0.5234776123684135 0.0 62-63 0.0 0.0 0.0 0.6120679399410245 0.0 64-65 0.0 0.0 0.0 0.6993651496748632 0.0 66-67 0.0 0.0 0.0 0.9286466981058881 0.0 68-69 0.0 0.0 0.0 1.1662982456387838 0.0 70-71 0.0 0.0 0.0 1.22340703164565 0.0 72-73 0.0 0.0 0.0 1.2996774728997766 0.0 74-75 0.0 0.0 0.0 1.3865749916652677 0.0 76-77 0.0 0.0 0.0 1.4566619785267203 0.0 78-79 0.0 0.0 0.0 1.5481441878114945 0.0 80-81 0.0 0.0 0.0 1.7162495068518786 0.0 82-83 0.0 0.0 0.0 1.9260872652344565 0.0 84-85 0.0 0.0 0.0 2.43940802476015 0.0 86-87 0.0 0.0 0.0 3.351502814529754 0.0 88-89 0.0 0.0 0.0 4.272249738202415 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 345 0.0 44.058517 2 GTACCCG 180 0.0 42.240627 1 TACCTGG 2390 0.0 42.134415 2 ACCTGGG 2405 0.0 40.488113 3 GTACCTG 2845 0.0 36.41305 1 ATAACGC 445 0.0 36.291733 3 TAACGCA 470 0.0 32.3317 4 AGTACGC 1305 0.0 32.031048 2 GAGTACG 1385 0.0 31.909212 1 TACGCAG 1345 0.0 31.422747 4 GTACGCA 1355 0.0 30.848366 3 TATCACG 280 0.0 30.536093 2 GTATAAC 565 0.0 29.437605 1 CCTGGGG 1825 0.0 28.882618 4 GTACACG 240 0.0 27.72041 1 CGCAGTG 1630 0.0 26.21992 6 ACGCAGT 1650 0.0 25.902102 5 TACCCGG 295 0.0 25.763031 2 TACACGG 210 1.2660166E-9 24.88126 2 TCACGCA 295 0.0 24.146027 4 >>END_MODULE