##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003784_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2419745 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7549940179647 31.0 31.0 34.0 30.0 34.0 2 31.95617968009026 31.0 31.0 34.0 30.0 34.0 3 32.00551049800702 33.0 31.0 34.0 30.0 34.0 4 35.74403046602018 37.0 35.0 37.0 35.0 37.0 5 35.601855154158805 37.0 35.0 37.0 33.0 37.0 6 35.6695998958568 37.0 35.0 37.0 33.0 37.0 7 35.62268751459348 37.0 35.0 37.0 33.0 37.0 8 35.623655385174885 37.0 35.0 37.0 33.0 37.0 9 37.22446993381534 39.0 37.0 39.0 33.0 39.0 10-11 37.13080448559662 39.0 37.0 39.0 33.0 39.0 12-13 37.102350247650065 39.0 37.0 39.0 33.0 39.0 14-15 38.23994739115072 40.0 37.5 41.0 33.0 41.0 16-17 38.25888037789106 40.0 38.0 41.0 33.0 41.0 18-19 38.29480854387549 40.0 38.0 41.0 33.5 41.0 20-21 38.238531539480405 40.0 37.5 41.0 33.0 41.0 22-23 38.154591289578036 40.0 37.0 41.0 33.0 41.0 24-25 38.0433295657187 40.0 37.0 41.0 33.0 41.0 26-27 37.85306013650199 40.0 37.0 41.0 33.0 41.0 28-29 37.71291747684157 40.0 37.0 41.0 32.5 41.0 30-31 37.53570624177341 39.0 36.5 41.0 32.0 41.0 32-33 37.29985804289295 39.0 36.0 41.0 31.5 41.0 34-35 37.26287398052274 39.0 36.0 41.0 31.0 41.0 36-37 37.1164947132859 39.0 36.0 41.0 31.0 41.0 38-39 36.89865378376647 39.0 36.0 40.5 31.0 41.0 40-41 36.93772794240715 39.0 36.0 41.0 30.5 41.0 42-43 36.82160475587304 39.0 36.0 41.0 30.0 41.0 44-45 36.60504082041703 39.0 35.0 41.0 30.0 41.0 46-47 36.45322709624361 39.0 35.0 41.0 30.0 41.0 48-49 36.23019636366642 39.0 35.0 41.0 29.5 41.0 50-51 36.008668268763856 39.0 35.0 41.0 29.0 41.0 52-53 35.84240508813946 39.0 35.0 40.0 28.0 41.0 54-55 35.669241387005655 39.0 35.0 40.0 28.0 41.0 56-57 35.39325466113165 38.0 34.5 40.0 27.5 41.0 58-59 35.13849661844533 38.0 34.0 40.0 27.0 41.0 60-61 34.762469805702665 38.0 34.0 40.0 26.0 41.0 62-63 34.540155884194405 37.5 34.0 40.0 26.0 41.0 64-65 34.10542164566928 37.0 33.0 39.5 25.5 41.0 66-67 33.7248631570682 36.5 33.0 39.0 25.0 41.0 68-69 33.404170687407145 36.0 32.5 39.0 24.5 40.5 70-71 32.95756722299251 35.5 32.0 38.5 23.5 40.0 72-73 32.55275121965331 35.0 31.5 38.0 22.5 40.0 74-75 32.185288325835984 35.0 31.0 37.0 22.0 39.5 76-77 31.161219467340565 34.0 30.5 36.0 20.5 39.0 78-79 31.58877856964267 35.0 31.0 36.0 21.0 39.0 80-81 31.367043634763167 35.0 31.0 36.0 20.0 38.5 82-83 31.057750713401617 34.5 31.0 35.5 20.0 37.0 84-85 30.70735098119843 34.0 31.0 35.0 19.5 37.0 86-87 30.454759902386407 34.0 31.0 35.0 18.0 36.5 88-89 30.119369396362014 34.0 30.5 35.0 15.0 36.0 90-91 29.80253125845905 34.0 30.0 35.0 9.0 36.0 92-93 29.56723353080593 34.0 30.0 35.0 6.0 35.5 94-95 29.232101729727717 34.0 30.0 35.0 2.0 35.0 96-97 28.88261015106964 34.0 29.0 35.0 2.0 35.0 98-99 28.555882954608855 34.0 29.0 35.0 2.0 35.0 100-101 27.637045225839913 33.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.07916837392445686 1101 2 0.1471897178095425 1101 3 0.06439916388422517 1101 4 0.03456901351115249 1101 5 -0.025200837438774215 1101 6 0.06884871197260622 1101 7 0.05553422665133212 1101 8 0.07449701935265551 1101 9 0.10976971150737569 1101 10-11 0.18583355597354512 1101 12-13 0.20809015228049077 1101 14-15 0.2474802863646275 1101 16-17 0.17524015143733607 1101 18-19 0.10297736785096134 1101 20-21 0.14449925387929596 1101 22-23 0.0748108560024292 1101 24-25 0.07902414671310964 1101 26-27 0.06839400416457408 1101 28-29 0.1253862462570865 1101 30-31 0.05182232345730142 1101 32-33 0.02808731388790875 1101 34-35 -0.04494741861572038 1101 36-37 -0.013525262169920893 1101 38-39 -0.16378502808165507 1101 40-41 -0.02274055437273148 1101 42-43 0.019630884214926425 1101 44-45 0.14917672560753203 1101 46-47 0.02785644181270186 1101 48-49 0.003899221046296475 1101 50-51 0.11076139957094 1101 52-53 -0.11064961702598453 1101 54-55 -0.020140762732303585 1101 56-57 -0.0728221271441285 1101 58-59 -0.026456753878541406 1101 60-61 -0.18847005168012743 1101 62-63 -0.24698283260344311 1101 64-65 -0.17305701647524785 1101 66-67 -0.18333433346364814 1101 68-69 -0.14284589038618378 1101 70-71 -0.05376306100882289 1101 72-73 -0.03033635011296809 1101 74-75 -0.2293402448212163 1101 76-77 -0.2746278862179068 1101 78-79 -0.22470859469306603 1101 80-81 -0.29647489337040156 1101 82-83 -0.24231696860045204 1101 84-85 -0.24657200879145336 1101 86-87 -0.3448095119777257 1101 88-89 -0.3823078489620535 1101 90-91 -0.2741594870989239 1101 92-93 -0.2015226114331803 1101 94-95 -0.17340496530022875 1101 96-97 -0.2376766750274406 1101 98-99 -0.15731372453684855 1101 100-101 -0.10874211227504205 1102 1 0.03635953517523305 1102 2 0.16584270106544707 1102 3 0.16888577370217206 1102 4 0.08578512453654241 1102 5 0.19721119107722274 1102 6 0.10930240410811365 1102 7 0.12671624529264136 1102 8 0.2155776146828714 1102 9 0.19577180897008617 1102 10-11 0.22412574730901014 1102 12-13 0.11040699090872153 1102 14-15 0.08009190695131707 1102 16-17 0.10139035779504724 1102 18-19 0.09722695825607985 1102 20-21 0.14520865038207376 1102 22-23 0.17768876016316426 1102 24-25 0.17946554047286867 1102 26-27 0.1536118504402353 1102 28-29 0.11457711498111678 1102 30-31 0.1396793238252556 1102 32-33 0.08617244299978921 1102 34-35 0.22403331711967667 1102 36-37 0.1455443879121816 1102 38-39 0.16784678106225925 1102 40-41 0.1458177405792327 1102 42-43 0.31538576211755753 1102 44-45 0.24234390962025998 1102 46-47 0.060062526139283534 1102 48-49 0.004178729586051588 1102 50-51 0.17776553131978545 1102 52-53 0.06711628270242898 1102 54-55 0.06589033852751669 1102 56-57 0.11025070167756468 1102 58-59 0.16335883557624697 1102 60-61 0.09701317455293434 1102 62-63 -0.13705662285794062 1102 64-65 -0.20728092272577925 1102 66-67 0.02861067457126154 1102 68-69 -0.026084069568014456 1102 70-71 -0.11886213147062108 1102 72-73 0.020637201104300118 1102 74-75 0.10095279499496712 1102 76-77 0.036224664603832224 1102 78-79 0.0316542449145949 1102 80-81 0.13882002834231955 1102 82-83 -0.06945414641421266 1102 84-85 0.05254616091005104 1102 86-87 0.0031455001655764647 1102 88-89 -0.06904166532187972 1102 90-91 0.04496287905956109 1102 92-93 0.03102772786338548 1102 94-95 -0.1524612564056902 1102 96-97 0.14654926053167472 1102 98-99 0.08828009135585191 1102 100-101 0.26650403711193604 1103 1 -0.07912083196460884 1103 2 0.024582196315350302 1103 3 -0.026808444831946332 1103 4 -0.023293636088176584 1103 5 0.02441081117677868 1103 6 -0.03676501593562875 1103 7 0.024953140243795247 1103 8 0.04483048610599383 1103 9 0.01836836092059002 1103 10-11 0.01044739276623119 1103 12-13 -0.11884858695954392 1103 14-15 -0.08270086419130251 1103 16-17 0.030731217477189432 1103 18-19 -0.11549214383371265 1103 20-21 -0.06811709850665437 1103 22-23 -0.026919644097588957 1103 24-25 4.1557565665328866E-4 1103 26-27 -0.11986550544347807 1103 28-29 -0.1885837058280302 1103 30-31 -0.0795920627537754 1103 32-33 -0.04715222714980172 1103 34-35 -0.18911215565022133 1103 36-37 -0.23497508064180295 1103 38-39 -0.060207019995985434 1103 40-41 -0.20971461717979878 1103 42-43 0.08801385087205915 1103 44-45 0.18931399629944679 1103 46-47 0.015720973530399363 1103 48-49 -0.21723996208381635 1103 50-51 -0.18235002812929935 1103 52-53 -0.13496956113068848 1103 54-55 -0.2202756383475517 1103 56-57 -0.3112060529760754 1103 58-59 -0.20789096240009997 1103 60-61 -0.13760327610357592 1103 62-63 -0.35145823167642476 1103 64-65 -0.3237281242039174 1103 66-67 -0.2788199953257333 1103 68-69 -0.3479646612084366 1103 70-71 -0.27860708068735107 1103 72-73 -0.14640670434594227 1103 74-75 -0.2854998347291726 1103 76-77 -0.33560346806802954 1103 78-79 -0.17092730066136497 1103 80-81 -0.24542953584323257 1103 82-83 -0.0933390046406366 1103 84-85 -0.37072746299696036 1103 86-87 -0.27617960071188463 1103 88-89 -0.22806729516540614 1103 90-91 -0.15348423565878022 1103 92-93 -0.25351217454335284 1103 94-95 -0.2060748789230047 1103 96-97 -0.11594223144131632 1103 98-99 -0.3307352002511301 1103 100-101 -0.13164360273353992 1104 1 0.18218998208381976 1104 2 0.06031421614168053 1104 3 0.09724504841422288 1104 4 0.0267456874913492 1104 5 -0.014899224270266131 1104 6 0.05696823127689754 1104 7 0.12544762716397173 1104 8 0.13751827701766217 1104 9 0.1439087741576941 1104 10-11 0.1488852445804909 1104 12-13 0.15444525745891724 1104 14-15 0.1748510109952761 1104 16-17 0.23190440906383003 1104 18-19 0.07900766151593785 1104 20-21 0.1428490096376791 1104 22-23 -0.0045016347217909924 1104 24-25 0.195995612639301 1104 26-27 0.18362714588824502 1104 28-29 0.22429310548632486 1104 30-31 0.1391874219233955 1104 32-33 0.135105014035247 1104 34-35 0.1064866783145888 1104 36-37 0.10233566605229782 1104 38-39 0.28153666640519504 1104 40-41 0.43236823852654993 1104 42-43 0.4119416770495832 1104 44-45 0.48479706501385067 1104 46-47 0.3560527814468415 1104 48-49 0.2571118577550706 1104 50-51 0.3127427149360855 1104 52-53 0.22962472147524693 1104 54-55 0.1758974827732871 1104 56-57 0.09982107672474427 1104 58-59 0.03676658568640789 1104 60-61 0.05710673697743829 1104 62-63 -0.21351116961545102 1104 64-65 0.01713016393825484 1104 66-67 0.032660366769661664 1104 68-69 -0.012161059106318817 1104 70-71 -0.002698863404489771 1104 72-73 0.04050768592443177 1104 74-75 -0.04516623313770651 1104 76-77 0.030402876050718675 1104 78-79 0.10347679199256632 1104 80-81 -0.014704999119292239 1104 82-83 0.05198957923645864 1104 84-85 0.10989361711387602 1104 86-87 0.21010647251801018 1104 88-89 0.12628556061232032 1104 90-91 -0.0017646778472624192 1104 92-93 0.15611744437574515 1104 94-95 0.24244161455945346 1104 96-97 0.2516465733177071 1104 98-99 0.16053417807391668 1104 100-101 0.16062219867405503 1105 1 -0.00468914906160478 1105 2 -6.905442897995329E-4 1105 3 -0.028064622290099095 1105 4 -0.04779887363118718 1105 5 -0.06262972127733946 1105 6 -0.019232124563835384 1105 7 0.004364540171913234 1105 8 0.09253553607336329 1105 9 -0.046734330409741176 1105 10-11 -0.047279284528514154 1105 12-13 0.00563917852743856 1105 14-15 0.04272966809081424 1105 16-17 0.075862352952619 1105 18-19 0.03962694226007102 1105 20-21 -0.03176444904588749 1105 22-23 0.0769663946432999 1105 24-25 -0.007801812931823804 1105 26-27 0.011943956806469203 1105 28-29 -0.12312912909990104 1105 30-31 -0.11018916347882168 1105 32-33 -3.540040488516638E-4 1105 34-35 0.12489246674967802 1105 36-37 0.02102267786541745 1105 38-39 0.1800580120828883 1105 40-41 0.16106766923336835 1105 42-43 0.16622818864040312 1105 44-45 0.262091808779914 1105 46-47 0.16084233189675246 1105 48-49 0.04012613904951223 1105 50-51 0.09966353127251892 1105 52-53 0.22514092020182375 1105 54-55 0.12806021621252484 1105 56-57 0.15205383435178987 1105 58-59 0.16696520998844733 1105 60-61 0.07746904281847122 1105 62-63 0.021623033300436134 1105 64-65 -0.08428210606738418 1105 66-67 -0.058500899454841715 1105 68-69 -0.09991913247051798 1105 70-71 -0.04274654571653258 1105 72-73 -0.004645955636654264 1105 74-75 0.042454911740435364 1105 76-77 0.12572000849089804 1105 78-79 0.16613102069953456 1105 80-81 0.007502374739669193 1105 82-83 0.033739785279163215 1105 84-85 0.06275234591487688 1105 86-87 0.15403755069117508 1105 88-89 0.2802681429622389 1105 90-91 0.22969940832408753 1105 92-93 0.34560064333716767 1105 94-95 0.24635785592450787 1105 96-97 0.29133546833082136 1105 98-99 0.17084497973820234 1105 100-101 0.23658821614370495 1106 1 -0.022633748190610703 1106 2 0.07526076576946039 1106 3 0.023049106559760446 1106 4 0.012706587923744905 1106 5 0.044377489403032655 1106 6 -0.012567599683421804 1106 7 -0.08928863324138092 1106 8 0.03140538686225369 1106 9 -0.09623814992855273 1106 10-11 -0.024178335003277596 1106 12-13 -0.05368651576826977 1106 14-15 0.07340897129635238 1106 16-17 0.0714734759625415 1106 18-19 -0.036493925877287836 1106 20-21 0.03672452075492316 1106 22-23 0.03191570570205471 1106 24-25 0.0068926255632035804 1106 26-27 -0.09063868044633239 1106 28-29 -0.10346931614923705 1106 30-31 -0.02611334424557299 1106 32-33 0.01621092777095612 1106 34-35 0.018940085797289896 1106 36-37 0.05360460118946264 1106 38-39 0.19255355002820096 1106 40-41 0.18088454650902008 1106 42-43 0.27410996203941806 1106 44-45 0.3532658646091491 1106 46-47 0.2004957194886714 1106 48-49 0.30443148637584727 1106 50-51 0.3067421348278856 1106 52-53 0.4047868115418609 1106 54-55 0.29990643595979094 1106 56-57 0.26060134631009646 1106 58-59 0.2798462694587087 1106 60-61 0.15577453293056465 1106 62-63 0.17715776593411903 1106 64-65 0.2368109188487253 1106 66-67 0.1951704791415736 1106 68-69 0.2587515321971381 1106 70-71 0.33606404898611686 1106 72-73 0.3515919968156851 1106 74-75 0.32091211171187695 1106 76-77 0.2256468307940338 1106 78-79 0.2481507251330335 1106 80-81 0.18413370193321654 1106 82-83 0.29265140089992414 1106 84-85 0.3121597850524509 1106 86-87 0.5017083581445902 1106 88-89 0.315045397192268 1106 90-91 0.3608463348608204 1106 92-93 0.37214451425885287 1106 94-95 0.45066463110834576 1106 96-97 0.431203034547714 1106 98-99 0.5055169294776185 1106 100-101 0.6113062142874881 1107 1 0.020076820276251794 1107 2 0.038349707713909 1107 3 0.04789976186313538 1107 4 0.04877799131057259 1107 5 -0.04430102410094605 1107 6 -0.04118083469606404 1107 7 0.025317429196292096 1107 8 0.09895502571504267 1107 9 0.10658655515974402 1107 10-11 0.13187579174579156 1107 12-13 0.03963427563270017 1107 14-15 0.16799100762262498 1107 16-17 0.08524517249814778 1107 18-19 0.09508681092346194 1107 20-21 0.002205244459680955 1107 22-23 0.027978093000953663 1107 24-25 0.09444513546075228 1107 26-27 0.031142727272246873 1107 28-29 0.06877347349888652 1107 30-31 0.06285126635009419 1107 32-33 0.08848746478728486 1107 34-35 0.07108002152619264 1107 36-37 0.12889271304939598 1107 38-39 0.24765318031823114 1107 40-41 0.3093433999044066 1107 42-43 0.20330093184811915 1107 44-45 0.32656741497331865 1107 46-47 0.2773165662957666 1107 48-49 0.2716761396845939 1107 50-51 0.24386071401331577 1107 52-53 0.3824107953431195 1107 54-55 0.37349836479818066 1107 56-57 0.3740063550239654 1107 58-59 0.42643249650294024 1107 60-61 0.27986587244923555 1107 62-63 0.34559768050794304 1107 64-65 0.24752193056460925 1107 66-67 0.2544371538673147 1107 68-69 0.2609174080672716 1107 70-71 0.22566280788887383 1107 72-73 0.26038217885000137 1107 74-75 0.1843899039288246 1107 76-77 0.25813119115548133 1107 78-79 0.3570454232703817 1107 80-81 0.3407036093546161 1107 82-83 0.29903846810251977 1107 84-85 0.330620602846885 1107 86-87 0.3588439117525368 1107 88-89 0.18042437510033338 1107 90-91 0.17630993303544074 1107 92-93 0.10964438833583401 1107 94-95 0.1441032864520153 1107 96-97 0.28339938378764273 1107 98-99 0.31756473992822265 1107 100-101 0.4300518699434477 1108 1 -0.025432397307131538 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5703.0 13 8031.0 14 9358.0 15 12037.0 16 14813.0 17 19112.0 18 16961.0 19 12840.0 20 11727.0 21 11794.0 22 12611.0 23 13865.0 24 16502.0 25 19790.0 26 25156.0 27 31617.0 28 40328.0 29 50697.0 30 62938.0 31 78322.0 32 96954.0 33 123448.0 34 162228.0 35 219087.0 36 316628.0 37 459100.0 38 452347.0 39 107085.0 40 100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27475518643376 20.345937291836627 14.710232448325867 20.669075073403757 2 14.737986258201168 23.159208555044685 38.50604949114015 23.59675569561399 3 22.549646655308152 25.874803565322978 30.034112760222975 21.541437019145896 4 12.187399911974197 16.889258992166532 40.9542327807269 29.96910831513238 5 11.811203246623094 39.61074410733362 35.07621670878543 13.501835937257853 6 27.77123209263786 39.275791457364306 17.578174559716004 15.374801890281828 7 26.297316452766715 33.34016600922824 22.796493018892487 17.566024519112553 8 24.080099349311602 32.67484796951745 20.19609504307272 23.04895763809823 9 23.031683090573594 20.38532985913805 23.97409644404679 32.60889060624157 10-11 24.51008267400077 25.907771273419307 28.532985913804964 21.04916013877495 12-13 27.782788637395395 23.858531344941582 27.547474537084394 20.811205480578632 14-15 22.304952624429323 26.561480377582907 29.0794505285144 22.05411646947337 16-17 20.010951567210594 29.548051550886562 29.241820935677104 21.199175946225736 18-19 19.37328106887296 31.44680534519133 29.577682772358244 19.602230813577464 20-21 21.619728206060806 27.647056392282636 28.347475490601287 22.385739911055268 22-23 20.21843210751546 28.03254061894952 29.110732742499728 22.638294531035292 24-25 22.490076433673796 27.96455824890639 28.90271495550151 20.642650361918303 26-27 21.998865513788967 25.97655188517925 31.721949621786266 20.302632979245516 28-29 19.87532585194759 30.175512631709907 30.117110373654587 19.832051142687916 30-31 21.09696479733628 27.79496469483475 28.976666848524225 22.13140365930474 32-33 21.24258013454689 28.12315245995985 29.739946954096798 20.89432045139646 34-35 23.021020627587337 26.70233234880401 29.943633233516536 20.33301379009212 36-37 22.391880119747977 26.403416764923804 31.267139885439583 19.93756322988863 38-39 21.073884967681824 28.26744113693161 31.090234757681394 19.56843913770517 40-41 20.816890296105893 28.859325575960064 29.651587737677097 20.672196390256946 42-43 20.036073926751083 29.50432242353303 28.059050802735797 22.40055284698009 44-45 21.433215250938524 27.144051015562326 28.320597215040976 23.102136518458174 46-47 22.86829802943297 28.729309624784083 28.328753094218605 20.073639251564337 48-49 22.498788081416134 28.204042042933118 28.77863403034322 20.51853584530753 50-51 19.81297754957775 28.643603705320825 30.860708692510403 20.682710052591023 52-53 21.195010595201317 30.168810330915363 27.716716035173384 20.91946303870994 54-55 20.244924273497737 28.439483371578 28.636261727818525 22.679330627105738 56-57 22.270516755640614 27.2803151523117 28.611070297374052 21.83809779467363 58-59 24.351530821633315 27.025793287521033 27.657410388484298 20.96526550236136 60-61 21.844654676926503 28.161017756473033 30.49398939037976 19.500338176220705 62-63 21.01249877045691 28.25539614431819 29.666911610937024 21.065193474287877 64-65 20.263395629192452 30.796718844437077 28.419098953873718 20.52078657249676 66-67 21.457085353887887 27.95404369627285 27.55513811839511 23.033732831444155 68-69 20.54623993716866 29.080430311986525 27.91478499901826 22.458544751826555 70-71 22.708538747512037 28.47054466592034 27.581440112953604 21.239476473614022 72-73 22.043004531469226 26.74277661489124 30.142742313756198 21.071476539883335 74-75 20.41601620010435 30.754596314682892 28.353612736918777 20.47577474829398 76-77 21.459478000690158 27.936922844856976 27.868092993654265 22.7355061607986 78-79 21.47471872964654 28.4785612975961 28.762249270168315 21.284470702589044 80-81 22.889818835383906 27.854953418414347 28.32550120199212 20.92972654420963 82-83 21.807821930913924 27.282243716770637 30.025477996557477 20.884456355757965 84-85 20.82481912758466 28.774857365314027 29.390277140367495 21.010046366733818 86-87 21.30842697360577 29.181400902145388 27.968821451958558 21.54135067229028 88-89 20.624363956082103 29.198850704155504 27.255627406635206 22.92115793312718 90-91 21.551539591987872 26.675846746664504 28.09970277700175 23.672910884345868 92-93 22.327619286133604 28.54616314944776 28.174222131854332 20.951995432564306 94-95 21.54206177173758 27.927214568657877 28.83951209130001 21.69121156830453 96-97 19.053313471042735 28.342467879274796 30.808764287386598 21.795454362295867 98-99 20.629042456904568 29.78662026838814 27.688262254972866 21.896075019734422 100-101 19.606626362617416 28.461387133783656 28.39660391269991 23.53538259089902 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 189.0 1 135.0 2 224.5 3 359.0 4 275.5 5 228.5 6 328.5 7 581.5 8 916.5 9 1154.0 10 1212.5 11 1233.0 12 1385.5 13 1594.5 14 1856.5 15 2107.5 16 2318.5 17 2608.5 18 2856.5 19 3167.0 20 3621.0 21 4151.0 22 4933.5 23 5986.0 24 7413.0 25 9553.5 26 12470.0 27 16446.5 28 22115.0 29 28991.0 30 36074.0 31 42768.5 32 48973.0 33 54184.5 34 58424.5 35 62860.5 36 67058.0 37 71362.5 38 74787.5 39 75738.0 40 77123.5 41 78583.0 42 79962.0 43 82774.0 44 83465.5 45 84797.0 46 91535.5 47 132210.5 48 221335.5 49 214855.5 50 125809.5 51 83208.5 52 62842.5 53 51447.5 54 44110.5 55 38088.0 56 31953.0 57 25578.0 58 20138.5 59 16011.0 60 12437.5 61 9201.5 62 6813.0 63 4757.0 64 3079.0 65 2043.5 66 1282.5 67 741.0 68 426.0 69 240.5 70 148.0 71 79.0 72 41.5 73 25.5 74 15.0 75 9.0 76 8.0 77 5.5 78 3.5 79 2.5 80 0.0 81 2.0 82 3.5 83 1.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05256752261085363 2 0.004959200246306946 3 0.04140932205666299 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.066333435961227E-5 14-15 0.0015704134113305329 16-17 0.0 18-19 0.0 20-21 0.010951567210594504 22-23 0.0 24-25 0.0 26-27 0.006095683636085621 28-29 0.013141880652713408 30-31 0.011116873885471402 32-33 0.005889050292489498 34-35 0.006013030298647171 36-37 0.02056001768781421 38-39 0.0437856055080184 40-41 0.006384970317120192 42-43 0.011116873885471402 44-45 0.010249013842367687 46-47 0.004607923562193537 48-49 0.0011571467241382873 50-51 0.0014670967395324713 52-53 7.232167025864295E-4 54-55 7.232167025864295E-4 56-57 0.008947223777712114 58-59 0.006694920332514376 60-61 0.0061370103048048455 62-63 0.006075020301726008 64-65 0.0675071133528533 66-67 0.031056991542497248 68-69 0.02355620116995799 70-71 0.014237037373772857 72-73 0.0 74-75 3.099500153941841E-4 76-77 4.1326668719224546E-4 78-79 3.7194001847302093E-4 80-81 4.959200246306946E-4 82-83 4.1326668719224546E-4 84-85 5.992366964287559E-4 86-87 0.0028308768072668813 88-89 1.0331667179806137E-4 90-91 5.372466933499192E-4 92-93 2.686233466749596E-4 94-95 0.0012398000615767364 96-97 0.0034921035067744743 98-99 0.0047525669027108225 100-101 0.005661753614533763 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2419745.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.35265425293703 #Duplication Level Percentage of deduplicated Percentage of total 1 80.06471532059263 41.91600359038816 2 12.721497627849063 13.32008333780681 3 3.1860858835536083 5.004001580455363 4 1.2445020250423908 2.6061193693649702 5 0.6378452966856206 1.6696447142122164 6 0.4213018398444525 1.323376173450172 7 0.27491192495688294 1.0074658270093941 8 0.2001611770758991 0.8383175118652361 9 0.14569945551226376 0.6864977897347252 >10 0.9606986031189655 9.59597920108941 >50 0.08948088260142398 3.181229875489893 >100 0.04607669319678238 4.2173871978448725 >500 0.002713536124809376 0.9766263449031816 >1k 0.0028731558968569555 3.3103931154416686 >5k 7.182889742142389E-4 2.579615551808721 >10k+ 7.182889742142389E-4 7.767258819135163 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 58009 2.3973187257334967 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 24837 1.0264304709793801 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 20010 0.8269466410716831 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 18413 0.7609479511270816 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 14915 0.6163872639472341 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 13774 0.5692335349385989 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 12625 0.5217491925802099 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12007 0.49620931131172913 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 11307 0.46728064320827195 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9592 0.3964054063548018 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9117 0.3767752387131702 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8753 0.3617323312993725 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6181 0.2554401393535269 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6131 0.2533738059175657 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6101 0.25213400585598894 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5638 0.23299975823898803 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5162 0.2133282639286371 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5064 0.2092782503941531 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4981 0.20584813689045744 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4779 0.1975001498091741 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4662 0.19266492956902484 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4614 0.19068124947050205 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4008 0.16563728822665197 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3812 0.15753726115768396 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3801 0.15708266780177252 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3498 0.14456068717984746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2922 0.12075652599757414 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2761 0.11410293233377897 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2473 0.10220085174264232 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.132666871922454E-5 0.0 10-11 0.0 0.0 0.0 4.132666871922454E-5 0.0 12-13 0.0 0.0 0.0 4.132666871922454E-5 0.0 14-15 0.0 2.066333435961227E-5 0.0 1.446433405172859E-4 0.0 16-17 0.0 4.132666871922454E-5 0.0 3.3061334975379635E-4 0.0 18-19 0.0 4.132666871922454E-5 0.0 5.37246693349919E-4 0.0 20-21 0.0 4.132666871922454E-5 0.0 7.232167025864295E-4 0.0 22-23 0.0 4.132666871922454E-5 0.0 0.0011778100584978995 0.0 24-25 0.0 6.199000307883682E-5 0.0 0.0019216900954439414 0.0 26-27 0.0 8.265333743844909E-5 0.0 0.002934193479064943 0.0 28-29 0.0 8.265333743844909E-5 0.0 0.0048352202401492714 0.0 30-31 0.0 8.265333743844909E-5 0.0 0.009587787142860096 0.0 32-33 0.0 8.265333743844909E-5 0.0 0.016654647493847492 0.0 34-35 0.0 8.265333743844909E-5 0.0 0.022461044448898543 0.0 36-37 0.0 8.265333743844909E-5 0.0 0.028660044756782223 0.0 38-39 0.0 8.265333743844909E-5 0.0 0.03820650523092309 0.0 40-41 0.0 8.265333743844909E-5 0.0 0.04998460581590208 0.0 42-43 0.0 8.265333743844909E-5 0.0 0.061452756385486904 0.0 44-45 0.0 8.265333743844909E-5 0.0 0.06492419655790177 0.0 46-47 0.0 8.265333743844909E-5 0.0 0.07104054352834699 0.0 48-49 0.0 8.265333743844909E-5 0.0 0.08056634066812826 0.0 50-51 0.0 8.265333743844909E-5 0.0 0.08998882113611145 0.0 52-53 0.0 8.265333743844909E-5 0.0 0.11218124223833503 0.0 54-55 0.0 8.265333743844909E-5 0.0 0.12315347278328914 0.0 56-57 0.0 8.265333743844909E-5 0.0 0.15511965103760933 0.0 58-59 0.0 2.0663334359612273E-4 0.0 0.2286397946891098 0.0 60-61 0.0 2.0663334359612273E-4 0.0 0.28548462751240317 0.0 62-63 0.0 2.0663334359612273E-4 0.0 0.30974338205058793 0.0 64-65 0.0 2.0663334359612273E-4 0.0 0.3690264883283156 0.0 66-67 0.0 2.0663334359612273E-4 0.0 0.5572488010100238 0.0 68-69 0.0 2.0663334359612273E-4 0.0 0.7533025174140251 0.0 70-71 0.0 2.0663334359612273E-4 0.0 0.8662689663580254 0.0 72-73 0.0 2.0663334359612273E-4 0.0 0.9857030389565843 0.0 74-75 0.0 2.0663334359612273E-4 0.0 1.1310282695077374 0.0 76-77 0.0 2.0663334359612273E-4 0.0 1.1830585454252411 0.0 78-79 0.0 2.0663334359612273E-4 0.0 1.26998919307613 0.0 80-81 0.0 2.0663334359612273E-4 0.0 1.3959322159979668 0.0 82-83 0.0 2.0663334359612273E-4 0.0 1.4942690242153616 0.0 84-85 0.0 2.0663334359612273E-4 0.0 1.7154286918662915 0.0 86-87 0.0 2.0663334359612273E-4 0.0 2.2506503784489684 0.0 88-89 0.0 2.0663334359612273E-4 0.0 2.935619249135756 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 365 0.0 53.363895 2 TACCTGG 2650 0.0 42.48729 2 TAACGCA 460 0.0 40.25843 4 GTACCTG 3030 0.0 38.278522 1 ACCTGGG 2915 0.0 37.64463 3 GTACCCG 315 0.0 36.216686 1 ATAACGC 590 0.0 34.621513 3 GTATAAC 785 0.0 34.515427 1 GTACACG 315 0.0 30.180573 1 CCTGGGG 2165 0.0 27.415882 4 GAGTACG 970 0.0 26.952494 1 TACACGG 300 0.0 26.920568 2 GTACCGG 195 5.311449E-10 26.814278 1 AGTACGC 865 0.0 26.362202 2 TACCCGG 385 0.0 24.6789 2 GTACGCA 930 0.0 24.518162 3 TACGCAG 940 0.0 24.247305 4 ACACGGG 350 0.0 21.716087 3 CTGGGGG 1310 0.0 21.38606 5 TACCGGG 255 1.1914381E-8 20.493166 2 >>END_MODULE