##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003783_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 942785 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.667308028871908 31.0 31.0 34.0 30.0 34.0 2 31.78208287149244 31.0 31.0 34.0 30.0 34.0 3 31.861925041234215 31.0 31.0 34.0 30.0 34.0 4 35.56661274839969 37.0 35.0 37.0 33.0 37.0 5 35.45960107553684 37.0 35.0 37.0 33.0 37.0 6 35.5354030876605 37.0 35.0 37.0 33.0 37.0 7 35.512606797944386 37.0 35.0 37.0 33.0 37.0 8 35.52905593534051 37.0 35.0 37.0 33.0 37.0 9 37.07274192949612 39.0 37.0 39.0 33.0 39.0 10-11 36.96006936894413 39.0 37.0 39.0 33.0 39.0 12-13 36.93999427228901 39.0 37.0 39.0 33.0 39.0 14-15 37.91123904177516 39.5 37.0 41.0 33.0 41.0 16-17 38.010114713322764 40.0 37.0 41.0 33.0 41.0 18-19 38.13273280758604 40.0 37.0 41.0 33.0 41.0 20-21 38.04309253965644 40.0 37.0 41.0 33.0 41.0 22-23 37.92200713842499 40.0 37.0 41.0 33.0 41.0 24-25 37.701378363041414 39.5 36.5 41.0 32.0 41.0 26-27 37.44306549213235 39.0 36.0 41.0 31.5 41.0 28-29 37.376593284789216 39.0 36.0 41.0 31.5 41.0 30-31 37.226258903143346 39.0 36.0 41.0 31.0 41.0 32-33 36.92043466962245 39.0 35.5 40.0 30.5 41.0 34-35 36.73383592229405 39.0 35.0 40.0 30.0 41.0 36-37 36.52523321860233 38.5 35.0 40.0 30.0 41.0 38-39 36.25109171232042 38.0 35.0 40.0 30.0 41.0 40-41 36.52091463058916 39.0 35.0 40.5 30.0 41.0 42-43 36.399517917658855 39.0 35.0 41.0 30.0 41.0 44-45 36.058200968407434 39.0 35.0 41.0 29.5 41.0 46-47 35.94909337759935 39.0 35.0 40.0 28.5 41.0 48-49 35.60571498273732 38.5 35.0 40.0 27.5 41.0 50-51 35.361866173093546 38.0 34.5 40.0 27.0 41.0 52-53 35.265170744125115 38.0 34.0 40.0 27.0 41.0 54-55 35.127224128512864 38.0 34.0 40.0 26.5 41.0 56-57 34.674643741680235 38.0 33.5 40.0 25.0 41.0 58-59 34.310921365953 38.0 33.0 40.0 24.0 41.0 60-61 33.74944234369448 37.0 32.0 40.0 22.0 41.0 62-63 33.60909645359229 37.0 32.0 40.0 22.0 41.0 64-65 33.23565075812619 36.5 32.0 39.5 22.0 40.5 66-67 32.75213436785694 36.0 31.5 39.0 21.0 40.0 68-69 32.273963841172694 35.5 31.0 38.5 20.0 40.0 70-71 31.631623859098312 35.0 30.5 37.5 18.5 39.0 72-73 31.118314886214776 34.5 30.0 37.0 17.0 39.0 74-75 30.813106911968262 34.0 30.0 36.5 14.5 39.0 76-77 29.803223428459297 33.5 29.0 35.0 12.0 37.0 78-79 30.2172690486166 34.0 30.0 35.5 11.5 37.0 80-81 29.801304114936066 34.0 29.0 35.0 7.0 37.0 82-83 29.439104886055674 34.0 29.0 35.0 6.5 36.5 84-85 29.068902241762437 34.0 29.0 35.0 2.0 36.0 86-87 29.018263973228255 34.0 29.0 35.0 2.0 36.0 88-89 28.73739187619659 34.0 29.0 35.0 2.0 35.0 90-91 28.2993773766023 34.0 29.0 35.0 2.0 35.0 92-93 28.012909624145486 34.0 27.5 35.0 2.0 35.0 94-95 27.53258484171895 33.0 27.0 35.0 2.0 35.0 96-97 27.156881473506687 33.0 25.5 35.0 2.0 35.0 98-99 26.880663671993084 33.5 25.0 35.0 2.0 35.0 100-101 25.88472716473003 33.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.07633529427767627 1101 2 0.21618927703883273 1101 3 0.2587863030084172 1101 4 0.17543108840425248 1101 5 0.16245095864962167 1101 6 0.17755502401022483 1101 7 0.14858127711328706 1101 8 0.09053151862618591 1101 9 0.11446404832388879 1101 10-11 0.30756786509984835 1101 12-13 0.30681102665788273 1101 14-15 0.37746893340375465 1101 16-17 0.244594103591794 1101 18-19 0.2368030515433972 1101 20-21 0.19448950464915526 1101 22-23 0.15139275709338307 1101 24-25 0.19927012178672499 1101 26-27 0.3954931939605473 1101 28-29 0.3795052353042436 1101 30-31 0.4807175182264345 1101 32-33 0.43195020426773567 1101 34-35 0.6181858515311589 1101 36-37 0.4272995425326229 1101 38-39 0.31160461308349596 1101 40-41 0.7201947840776413 1101 42-43 0.6743285623671724 1101 44-45 0.6650580917821998 1101 46-47 0.8076889111748855 1101 48-49 0.7019502088773066 1101 50-51 0.48610354147837853 1101 52-53 0.6041118672846153 1101 54-55 0.6506915521954042 1101 56-57 0.6318128694340288 1101 58-59 0.6550369010805142 1101 60-61 0.5078607960292771 1101 62-63 0.4646545265183377 1101 64-65 0.336159695991185 1101 66-67 0.37301122792221264 1101 68-69 0.4799375442824996 1101 70-71 0.5727282878421356 1101 72-73 0.5733968503299245 1101 74-75 0.4437532416040888 1101 76-77 0.6443076012420512 1101 78-79 0.7365779841326159 1101 80-81 0.9073190878891921 1101 82-83 1.0314527071589126 1101 84-85 0.9216382421957015 1101 86-87 0.9495376603220116 1101 88-89 1.0575636918586397 1101 90-91 1.1030221453675217 1101 92-93 0.8899604673244816 1101 94-95 0.8695063747699514 1101 96-97 0.9678293161498068 1101 98-99 0.9782061048008366 1101 100-101 0.8718081770245263 1102 1 0.038770930407260096 1102 2 0.15155722383136805 1102 3 0.15164459522038953 1102 4 0.006733384048963842 1102 5 0.05417751947347682 1102 6 0.005531853016876198 1102 7 -0.006648287357855054 1102 8 0.03282662697203165 1102 9 0.07058397966909524 1102 10-11 0.07432392603098492 1102 12-13 0.02077530470980804 1102 14-15 0.21410404389506255 1102 16-17 0.18649096048945069 1102 18-19 0.04658501893237599 1102 20-21 0.15495131541937468 1102 22-23 0.02612993795585794 1102 24-25 0.18130321554342999 1102 26-27 0.027876266262630622 1102 28-29 -0.18476074839023937 1102 30-31 -0.17147085336764434 1102 32-33 -0.27679925936668326 1102 34-35 -0.15327413559607095 1102 36-37 -0.08471772843926573 1102 38-39 0.10849503359411727 1102 40-41 0.03976274159747817 1102 42-43 0.23783692967032266 1102 44-45 0.42498487787960215 1102 46-47 0.1272515050388634 1102 48-49 -0.11592685613020137 1102 50-51 0.1644048716650346 1102 52-53 0.23644666668387515 1102 54-55 0.12146096862964839 1102 56-57 -0.01900508808581236 1102 58-59 0.089501071859317 1102 60-61 0.09491024893637956 1102 62-63 0.09553041152155117 1102 64-65 0.08106958657261032 1102 66-67 0.19048333691169717 1102 68-69 0.20580109287721626 1102 70-71 0.14543800678657348 1102 72-73 0.1736261466182789 1102 74-75 0.2500434690230975 1102 76-77 0.15423170551152054 1102 78-79 0.37189363528043984 1102 80-81 0.27505937967207217 1102 82-83 0.20074711822950064 1102 84-85 0.12347395341647172 1102 86-87 0.12295404092703066 1102 88-89 0.29884775107339934 1102 90-91 0.39448588705385745 1102 92-93 0.06865200347552403 1102 94-95 0.138056578588877 1102 96-97 0.2809944094774153 1102 98-99 0.4029325583515764 1102 100-101 0.7814104619419275 1103 1 -0.06671261894468117 1103 2 -0.1226202437658408 1103 3 -0.2271191136529893 1103 4 -0.1331968253229192 1103 5 -0.19627113456440526 1103 6 -0.16106834638492273 1103 7 -0.02165327240272319 1103 8 -0.05196898618848422 1103 9 -0.10568720677158439 1103 10-11 -0.0883630131514721 1103 12-13 -0.1749375567059488 1103 14-15 -0.33083613556654967 1103 16-17 -0.2669736456920049 1103 18-19 -0.5576273439788935 1103 20-21 -0.24180840541811222 1103 22-23 -0.27192589454663363 1103 24-25 -0.2814834245363329 1103 26-27 -0.5410918294502238 1103 28-29 -0.3864307384201524 1103 30-31 -0.33991551936964015 1103 32-33 -0.2658570858871201 1103 34-35 -0.2551435274205929 1103 36-37 -0.6536608989278037 1103 38-39 -0.5163304898355889 1103 40-41 -0.4321216053616368 1103 42-43 -0.21689886295181537 1103 44-45 -0.010982220824281796 1103 46-47 -0.36088408818147855 1103 48-49 -0.3675469957114572 1103 50-51 -0.26505674348950237 1103 52-53 -0.24074675305689652 1103 54-55 -0.06914222179906204 1103 56-57 -0.051831608524494754 1103 58-59 -0.30573821029422277 1103 60-61 -0.4968395018113725 1103 62-63 -0.4532981029350722 1103 64-65 -0.38897029378631487 1103 66-67 -0.3808277298879048 1103 68-69 -0.5687502530848931 1103 70-71 -0.2915759513390519 1103 72-73 -0.07335790123815755 1103 74-75 -0.2489096715551682 1103 76-77 -0.35005543307272546 1103 78-79 -0.16801164886711817 1103 80-81 0.03485499283258875 1103 82-83 0.010667357511653819 1103 84-85 -0.04955695386169623 1103 86-87 -0.2131376838984913 1103 88-89 -0.21673051413098676 1103 90-91 -0.2586546912113512 1103 92-93 -0.025116690442722245 1103 94-95 -0.22243694085279841 1103 96-97 -0.1931332076711385 1103 98-99 0.2592884905549653 1103 100-101 0.49364874708649253 1104 1 0.1814856780234635 1104 2 0.17196622768251046 1104 3 0.17617041024191238 1104 4 0.15254861151359478 1104 5 -0.018598805258278617 1104 6 0.03030891914534095 1104 7 -0.059677716688334215 1104 8 -0.0042215844084054766 1104 9 0.04965599812163646 1104 10-11 0.04411561415010112 1104 12-13 0.015509328640440856 1104 14-15 -0.02872242808631853 1104 16-17 0.2768553775662852 1104 18-19 0.02519829817120467 1104 20-21 -0.10069771561631313 1104 22-23 0.04658939797192119 1104 24-25 0.2216820486673825 1104 26-27 -0.01885901392354583 1104 28-29 0.04551037626738008 1104 30-31 0.14954259474488651 1104 32-33 0.29227471554939655 1104 34-35 0.3392434210898898 1104 36-37 0.2949216644392081 1104 38-39 0.20025729238276568 1104 40-41 0.2874469065994347 1104 42-43 0.2571770865992917 1104 44-45 0.5681521864036014 1104 46-47 0.636747561220254 1104 48-49 0.5541696284137387 1104 50-51 0.8051518288493753 1104 52-53 0.718254130077419 1104 54-55 0.6247699663024875 1104 56-57 0.7889304309948528 1104 58-59 0.36236440773262046 1104 60-61 0.45852466086462584 1104 62-63 0.10335575081352033 1104 64-65 0.33794192217818164 1104 66-67 0.5666500070107574 1104 68-69 0.5496212423922202 1104 70-71 0.5676392342100094 1104 72-73 0.46181785945061904 1104 74-75 0.41713472260136797 1104 76-77 0.47614821600434354 1104 78-79 0.5552662695041342 1104 80-81 0.31166429760357417 1104 82-83 0.05897868113495264 1104 84-85 0.31379769664607693 1104 86-87 0.4850048266015996 1104 88-89 0.29101417404022456 1104 90-91 0.4266769202725449 1104 92-93 0.630992139810143 1104 94-95 0.367129111900244 1104 96-97 0.30811820213346763 1104 98-99 0.5186743669476606 1104 100-101 0.503018571998151 1105 1 -0.039542979318383686 1105 2 -0.12214693169592294 1105 3 -0.20314814753144717 1105 4 -0.15149720748424755 1105 5 -0.09529687510694629 1105 6 0.06920408288164737 1105 7 -0.068045489320113 1105 8 -0.05032155482255263 1105 9 0.058483374898415263 1105 10-11 0.010756168354554063 1105 12-13 -0.10340254459068632 1105 14-15 -0.20339991436797078 1105 16-17 -0.12693663574176384 1105 18-19 -0.14051039467366877 1105 20-21 -0.3075567957753762 1105 22-23 -0.21430015003438285 1105 24-25 -0.04529306758269058 1105 26-27 -0.13502442675785176 1105 28-29 -0.21091059135751067 1105 30-31 0.02458515929879468 1105 32-33 -0.23178472410569384 1105 34-35 -0.11924799808211617 1105 36-37 0.02690918255821373 1105 38-39 0.14934171254654416 1105 40-41 0.07525213864371239 1105 42-43 0.17818006565288158 1105 44-45 0.19962409193727382 1105 46-47 0.2898543549882433 1105 48-49 0.29865577423291967 1105 50-51 0.06252177669268377 1105 52-53 0.16588251686687272 1105 54-55 0.33311578213706383 1105 56-57 0.22808591303028436 1105 58-59 0.6055852465939182 1105 60-61 0.3596148716684908 1105 62-63 0.381219019510759 1105 64-65 0.44182827528442203 1105 66-67 0.4185253593894416 1105 68-69 0.4550451295268445 1105 70-71 0.29581501511838937 1105 72-73 0.32061332444162005 1105 74-75 0.5084843027241384 1105 76-77 0.41566654341740517 1105 78-79 0.44535575275649464 1105 80-81 0.05263549512357457 1105 82-83 0.24957391502135806 1105 84-85 0.32337402349719113 1105 86-87 0.42689932605174974 1105 88-89 0.4625349055365042 1105 90-91 0.34922152699467546 1105 92-93 0.6091962970877951 1105 94-95 0.32494694119172607 1105 96-97 0.5683136920584637 1105 98-99 0.5809330515122149 1105 100-101 0.8242083699025784 1106 1 -0.1318317374626723 1106 2 -0.24320856200579755 1106 3 -0.2742190783180618 1106 4 -0.12430669040322329 1106 5 -0.11473869361769573 1106 6 0.0956556579747101 1106 7 -0.05297362084159829 1106 8 0.017184617891125242 1106 9 -0.1962706975637616 1106 10-11 -0.32702058453050853 1106 12-13 -0.31806391805451284 1106 14-15 -0.5477328663392953 1106 16-17 -0.5658490660200357 1106 18-19 -0.3999962439346092 1106 20-21 -0.32813760417790405 1106 22-23 -0.42817926422130625 1106 24-25 -0.40141978848939885 1106 26-27 -0.32625879112759293 1106 28-29 -0.4664825961580803 1106 30-31 -0.2519116441400797 1106 32-33 -0.18896048782772112 1106 34-35 -0.10577632121890446 1106 36-37 -0.20211951641974935 1106 38-39 -0.12923304672399638 1106 40-41 -0.053074055830528266 1106 42-43 0.08570912008742226 1106 44-45 -0.09630943386071777 1106 46-47 -0.256369014434334 1106 48-49 -0.04154165068165838 1106 50-51 -0.12743217957724795 1106 52-53 0.07266243701017316 1106 54-55 0.18493498086913718 1106 56-57 0.12778345643039302 1106 58-59 -0.034643010038081457 1106 60-61 -0.09677098854916721 1106 62-63 -0.1377157319117046 1106 64-65 -0.15596050451851085 1106 66-67 -0.26527754184969154 1106 68-69 -0.06574844320670792 1106 70-71 0.206953418952736 1106 72-73 0.11527918251889346 1106 74-75 0.25138727454496745 1106 76-77 0.1944922881562654 1106 78-79 0.2452311518488699 1106 80-81 0.48776912895998237 1106 82-83 0.3114679507402869 1106 84-85 0.25514292321257415 1106 86-87 0.6036274947751359 1106 88-89 0.4768385291551276 1106 90-91 0.2568677929169638 1106 92-93 0.28802790240104414 1106 94-95 0.2463795713410697 1106 96-97 0.23043883825431166 1106 98-99 -0.09995739906403145 1106 100-101 -0.2788880518345458 1107 1 -0.033428706843348976 1107 2 -0.0038933984714653036 1107 3 -0.19935921990250804 1107 4 -0.010250167142480393 1107 5 -0.2353402740095376 1107 6 -0.008371455507734993 1107 7 -0.029141876700528258 1107 8 0.011146096607411948 1107 9 -0.22642646501162034 1107 10-11 -0.15064480246986278 1107 12-13 -0.14756855669491387 1107 14-15 0.14327566188243424 1107 16-17 -0.061537371488782355 1107 18-19 -0.07254844782602277 1107 20-21 0.058112863984014496 1107 22-23 -0.012043181063468467 1107 24-25 0.15281331623766192 1107 26-27 -0.07740079921988752 1107 28-29 -0.025609144162331177 1107 30-31 0.20861232882010938 1107 32-33 -0.17879907173768572 1107 34-35 -0.16282914216308342 1107 36-37 0.07836626680745695 1107 38-39 0.07846814010484593 1107 40-41 0.11283485367453494 1107 42-43 0.2966398566826456 1107 44-45 0.08693528128194572 1107 46-47 -0.01713213371278499 1107 48-49 0.06226596393359074 1107 50-51 0.017460097132548924 1107 52-53 -0.10452994223474832 1107 54-55 -0.17480677794612376 1107 56-57 0.010044571054848461 1107 58-59 -0.06379338057672612 1107 60-61 -0.12349459189168499 1107 62-63 0.11765968789906367 1107 64-65 0.41113400586087323 1107 66-67 0.39788686433680454 1107 68-69 0.03730243755168061 1107 70-71 0.1033637682475721 1107 72-73 0.14661226207265088 1107 74-75 0.1045778364006722 1107 76-77 0.008653495492129792 1107 78-79 0.07244870439044959 1107 80-81 -0.037000473977315096 1107 82-83 0.35018110409478354 1107 84-85 0.3269482169726672 1107 86-87 0.25429121023092804 1107 88-89 0.24921206403856289 1107 90-91 -0.005714250540744814 1107 92-93 -0.338183946239905 1107 94-95 -0.2990705087836929 1107 96-97 -0.29231536599322183 1107 98-99 -0.32725538335835225 1107 100-101 -0.26492608193198564 1108 1 -0.17180509060718663 1108 2 -0.051863878176263256 1108 3 -0.1274035003453129 1108 4 -0.09143097895059782 1108 5 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-0.609312078151703 2316 84-85 -0.7959491610950202 2316 86-87 -0.790062182232159 2316 88-89 -0.847583014291132 2316 90-91 -0.8144843192957971 2316 92-93 -0.6607004662980209 2316 94-95 -0.8500015288022915 2316 96-97 -1.0863526432687927 2316 98-99 -1.2207048079679161 2316 100-101 -1.54891302394309 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 10.0 5 23.0 6 46.0 7 152.0 8 416.0 9 719.0 10 1210.0 11 2009.0 12 3250.0 13 4370.0 14 4827.0 15 5726.0 16 6923.0 17 8638.0 18 8253.0 19 6757.0 20 6682.0 21 6890.0 22 7230.0 23 7776.0 24 8837.0 25 10406.0 26 12506.0 27 15266.0 28 19009.0 29 23321.0 30 28467.0 31 34456.0 32 42224.0 33 52017.0 34 66727.0 35 89265.0 36 127669.0 37 170197.0 38 137335.0 39 23163.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.406926751507854 16.5056127425228 13.37792961598589 18.70953088998345 2 14.874015898372612 25.209443132662525 34.19009311176594 25.726447857198924 3 29.38693996313554 24.041911306693436 28.892442573041826 17.6787061571292 4 10.761414320338147 15.906595883472901 46.65740333161856 26.674586464570393 5 11.578461685325921 39.99437835773798 34.98825288904681 13.438907067889286 6 25.388715348674406 38.1917404286237 16.583844672963615 19.835699549738276 7 25.418414590813388 35.697110157671155 21.29584157575693 17.588633675758526 8 21.206319574452287 25.825506345561287 21.701872643285586 31.26630143670084 9 19.830926457251653 25.05184108784081 26.629507257752298 28.48772519715524 10-11 26.649607280557074 21.593682546922153 27.24613777266291 24.510572399857868 12-13 33.05759001256914 22.201933632800692 24.7391504956061 20.00132585902406 14-15 24.278017349964493 24.14792178567697 33.180361880991995 18.39369898336654 16-17 18.204097434727963 29.129175792996282 31.362346664403866 21.30438010787189 18-19 17.889974914747263 34.7761684795579 28.12311396553827 19.210742640156557 20-21 21.244115829644816 26.010486088826774 24.78090333120288 27.964494750325535 22-23 18.592202888251297 27.15306246917378 27.899361996637623 26.3553726459373 24-25 27.23929634009875 25.170107712787114 26.316127218825073 21.274468728289058 26-27 23.39007160658965 22.29679952859835 35.76590304337287 18.547225821439124 28-29 18.866231193361287 32.21590140587332 29.24688578240656 19.670981618358834 30-31 20.36684118877295 27.267934307835358 27.316572775506152 25.04865172788554 32-33 22.655389375748168 26.258100889924425 28.716836008127544 22.369673726199863 34-35 26.697984064087336 23.891298894096668 28.27099643741179 21.139720604404204 36-37 24.733803141900047 22.3637718714277 34.34495123769007 18.557473748982183 38-39 21.703426144648123 27.27125608324791 32.1370585201551 18.88825925194887 40-41 18.91963421011597 30.69785844682501 30.222694374935692 20.15981296812333 42-43 18.380137322346837 30.277747574051432 23.685744518907683 27.656370584694045 44-45 21.928068192486457 24.545752051720378 25.977249015975122 27.54893073981804 46-47 26.976256494353706 26.759866432173386 27.13038137687724 19.133495696595677 48-49 24.75022937847714 26.542192380922074 28.02448118035778 20.683097060243007 50-51 17.201311681293056 27.630959963808372 36.39332194478019 18.77440641011838 52-53 21.087950121609335 32.69869841414636 24.658374815570664 21.554976648673644 54-55 17.909956198433456 26.985297120361228 28.870318405939067 26.234428275266247 56-57 24.90337281518072 22.594663347016105 27.506406009741486 24.995557828061692 58-59 29.531424449518536 24.44649529674153 25.372508148159934 20.649572105579995 60-61 23.563143711352126 24.91995292378012 34.009068377732035 17.507834987135716 62-63 18.675262406984082 28.27715693502278 31.578419776920914 21.46916088107222 64-65 18.48239961021301 33.76344006122798 27.55741980546742 20.196740523091588 66-67 20.948325119729045 26.083821737287394 24.46950231871402 28.49835082426954 68-69 18.70476271312319 27.67476897287089 27.327989560014004 26.292478753991915 70-71 26.793793926534942 25.540591433496886 25.320143217079966 22.34547142288821 72-73 22.55263270941844 23.352020904056975 34.27764698894285 19.817699397581737 74-75 19.025930323889064 32.16913022333501 27.925759710811633 20.879179741964293 76-77 20.136204723941738 27.474723475028956 26.381405048092223 26.00766675293708 78-79 22.44036087102483 26.711053385512656 27.487325972435617 23.361259771026898 80-81 25.928371447224734 25.150501211063396 26.52501971591057 22.3961076258013 82-83 23.60134306281327 23.10620665368719 32.75256279232327 20.539887491176273 84-85 20.796038476041574 27.09657779189695 30.550713139015567 21.556670593045908 86-87 19.098459775967413 30.267364646978955 28.865888068567546 21.768287508486083 88-89 18.48497319634868 29.36903759299605 23.276006542339587 28.869982668315686 90-91 21.23444087931903 24.00503831773223 26.197236880485804 28.56328392246294 92-93 25.36425537466608 26.441548633588237 27.343456237646237 20.850739754099447 94-95 22.912672336295437 26.63749645316722 28.011042251728334 22.438788958809006 96-97 16.23466101479162 27.682837309164356 35.35158736220372 20.730914313840305 98-99 19.897493997145364 32.25724837735957 24.33682180472304 23.508435820772025 100-101 16.741720955051235 26.184717355627907 28.43321653506971 28.64034515425115 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 202.0 1 152.0 2 401.5 3 625.0 4 396.5 5 267.0 6 340.5 7 533.0 8 819.0 9 957.0 10 877.5 11 791.0 12 793.0 13 850.0 14 978.0 15 1067.5 16 1127.0 17 1172.5 18 1267.0 19 1398.5 20 1437.0 21 1558.5 22 1751.0 23 1986.0 24 2340.0 25 2832.0 26 3439.0 27 4204.5 28 5417.0 29 7032.0 30 8610.0 31 9913.0 32 11168.5 33 12484.5 34 13668.0 35 15052.5 36 16377.0 37 17717.0 38 19091.0 39 19816.0 40 20693.0 41 20969.5 42 21170.5 43 22449.5 44 23229.5 45 24751.0 46 30871.5 47 74744.0 48 157875.0 49 147383.5 50 67566.5 51 33311.0 52 20959.0 53 16157.5 54 13783.5 55 11819.5 56 9987.5 57 8088.0 58 6432.5 59 5187.5 60 4124.0 61 3157.5 62 2368.0 63 1714.0 64 1142.5 65 768.5 66 520.0 67 323.0 68 182.5 69 115.5 70 60.5 71 26.5 72 15.0 73 8.0 74 7.0 75 6.0 76 3.5 77 2.0 78 0.5 79 0.5 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05876207194641408 2 0.004985229930471953 3 0.04391245087692316 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0017501339117614303 16-17 0.0 18-19 0.0 20-21 0.01140238760693053 22-23 0.0 24-25 0.0 26-27 0.006841432564158319 28-29 0.01405410565505391 30-31 0.012250937382330011 32-33 0.006841432564158319 34-35 0.007583913617632864 36-37 0.02147891618979937 38-39 0.04698844381274628 40-41 0.006151985871646239 42-43 0.011932731216555206 44-45 0.010659906553455983 46-47 0.004560955042772212 48-49 0.002121374438498703 50-51 0.0027047524090858467 52-53 0.001272824663099222 54-55 0.0011137215802118192 56-57 0.012250937382330011 58-59 0.009281013168431827 60-61 0.006205020232608707 62-63 0.007424810534745462 64-65 0.07764230444905254 66-67 0.036540674703140166 68-69 0.02704752409085847 70-71 0.016970995507989625 72-73 1.5910308288740276E-4 74-75 9.015841363619488E-4 76-77 0.0013788933850241573 78-79 0.0011137215802118192 80-81 0.0019622713556113006 82-83 0.0015379964679115598 84-85 0.0017501339117614303 86-87 0.006894466925120786 88-89 3.182061657748055E-4 90-91 0.0010606872192493516 92-93 5.833779705871434E-4 94-95 0.004507920681809744 96-97 0.00944011625131923 98-99 0.011508456328855466 100-101 0.014213208737941312 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 942785.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.65178504344846 #Duplication Level Percentage of deduplicated Percentage of total 1 84.67080841912427 36.96031928566619 2 9.424119921749488 8.22759314095778 3 2.377368358209191 3.113291176249308 4 1.0355564802791464 1.8081555550998152 5 0.5915002985909834 1.2910021943614594 6 0.3819085754106774 1.0002594624045908 7 0.2428351500333637 0.7420131439174965 8 0.1916280969191131 0.6691926795998586 9 0.14807849070964835 0.5817501401414265 >10 0.826498769939793 6.594136445859735 >50 0.055948188097762315 1.6811977702024847 >100 0.03835741998666679 3.4177408056919414 >500 0.004886295539661696 1.6071817624600127 >1k 0.007329443309492543 7.332673474432689 >5k 0.001221573884915424 3.953539158338097 >10k+ 0.0019545182158646787 21.019953804617195 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 54958 5.829324819550587 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 30889 3.276356751539322 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 30204 3.2036996770207415 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 20385 2.1622108964398032 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 19009 2.0162603350710926 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 17301 1.8350949580233034 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 12656 1.3424057446819795 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11695 1.240473702912117 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8549 0.9067815037362706 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8407 0.89171974522293 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7963 0.8446252326882586 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6234 0.6612324124800458 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5918 0.6277146963517664 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4736 0.502341467036493 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4662 0.49449238161404774 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4269 0.4528073738975482 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3649 0.3870447663040884 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3590 0.38078671171051726 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2976 0.31566051644860704 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2950 0.31290272967855876 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2883 0.30579612530958805 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2602 0.2759908144486813 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2465 0.26145939954496517 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2424 0.25711058194604286 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2272 0.2409881362134527 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2261 0.23982138027227842 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2247 0.23833641816532933 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2177 0.23091160763058388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2123 0.22518389664663735 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2098 0.22253217859851399 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2022 0.21447095573221892 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1862 0.19749996022422928 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1835 0.19463610473225604 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1692 0.1794682774969903 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1457 0.15454212784463053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1363 0.14457166798368665 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1283 0.13608617022969183 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1223 0.1297220469141957 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1186 0.1257975042029731 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1176 0.12473681698372376 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1162 0.12325185487677467 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1097 0.11635738795165387 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1014 0.10755368403188426 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 984 0.1043716223741362 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0606872192493517E-4 0.0 10-11 0.0 0.0 0.0 0.001007652858286884 0.0 12-13 0.0 0.0 0.0 0.0014849621069490922 0.0 14-15 0.0 0.0 0.0 0.001803168272723898 0.0 16-17 0.0 0.0 0.0 0.0025456493261984438 0.0 18-19 0.0 0.0 0.0 0.0032881303796729903 0.0 20-21 0.0 0.0 0.0 0.00461398940373468 0.0 22-23 0.0 0.0 0.0 0.007000535647045721 0.0 24-25 0.0 0.0 0.0 0.009015841363619489 0.0 26-27 0.0 0.0 0.0 0.011614525050780401 0.0 28-29 0.0 0.0 0.0 0.016811892425102223 0.0 30-31 0.0 0.0 0.0 0.024926149652359765 0.0 32-33 0.0 0.0 0.0 0.04035914869243783 0.0 34-35 0.0 0.0 0.0 0.0543071856255668 0.0 36-37 0.0 5.3034360962467586E-5 0.0 0.06608081375923461 0.0 38-39 0.0 1.0606872192493517E-4 0.0 0.08968110438753268 0.0 40-41 0.0 1.0606872192493517E-4 0.0 0.13476031120563012 0.0 42-43 0.0 1.0606872192493517E-4 0.0 0.20338677429106317 0.0 44-45 0.0 1.5910308288740276E-4 0.0 0.26384594578827625 0.0 46-47 0.0 2.1213744384987034E-4 0.0 0.4112284349029736 0.0 48-49 0.0 2.1213744384987034E-4 0.0 0.5209565277343191 0.0 50-51 0.0 2.1213744384987034E-4 0.0 0.5880449943518405 0.0 52-53 0.0 2.1213744384987034E-4 0.0 0.6708316318142524 0.0 54-55 0.0 2.1213744384987034E-4 0.0 0.7503301388969914 0.0 56-57 0.0 2.1213744384987034E-4 0.0 0.994712474212042 0.0 58-59 0.0 2.1213744384987034E-4 0.0 1.31297167434781 0.0 60-61 0.0 2.1213744384987034E-4 0.0 1.564884888919531 0.0 62-63 0.0 2.1213744384987034E-4 0.0 1.7582481689886877 0.0 64-65 0.0 2.1213744384987034E-4 0.0 2.0719464140816837 0.0 66-67 0.0 2.1213744384987034E-4 0.0 2.558801847717136 0.0 68-69 0.0 2.1213744384987034E-4 0.0 2.99861580317888 0.0 70-71 0.0 2.1213744384987034E-4 0.0 3.183917860381741 0.0 72-73 0.0 2.1213744384987034E-4 0.0 3.3012298668307194 0.0 74-75 0.0 2.1213744384987034E-4 0.0 3.4894488138865167 0.0 76-77 0.0 2.1213744384987034E-4 0.0 3.6821756816241242 0.0 78-79 0.0 2.1213744384987034E-4 0.0 3.917011831965931 0.0 80-81 0.0 2.1213744384987034E-4 0.0 4.3912981220532785 0.0 82-83 0.0 2.1213744384987034E-4 0.0 4.9737214741430975 0.0 84-85 0.0 2.1213744384987034E-4 0.0 6.064001866809506 0.0 86-87 0.0 2.1213744384987034E-4 0.0 6.832416722794699 0.0 88-89 0.0 2.1213744384987034E-4 0.0 7.725091086514953 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 135 0.0 66.87841 2 ATAACGC 200 0.0 49.894814 3 TACCTGG 1020 0.0 48.450607 2 ACCTGGG 1080 0.0 44.878933 3 TAACGCA 205 0.0 44.009174 4 GTATAAC 235 0.0 42.486217 1 AGTACGC 1085 0.0 40.292496 2 GTACCTG 1265 0.0 40.21517 1 GTACCCG 120 8.54925E-11 39.620083 1 GAGTACG 1170 0.0 39.010544 1 TACGCAG 1175 0.0 37.582752 4 GTACGCA 1175 0.0 36.80185 3 GTAGGCA 650 0.0 35.090855 3 CCTGGGG 745 0.0 34.417633 4 ACGCAGT 1315 0.0 33.220455 5 CGCAGTG 1315 0.0 33.220455 6 TAGGCAG 695 0.0 32.79442 4 AGTAGGC 770 0.0 31.473537 2 GAGTAGG 830 0.0 29.786667 1 AGGCAGT 815 0.0 27.965792 5 >>END_MODULE