##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003782_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2583306 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.709718089920436 31.0 31.0 34.0 30.0 34.0 2 31.893769456657477 31.0 31.0 34.0 30.0 34.0 3 31.95708483625246 31.0 31.0 34.0 30.0 34.0 4 35.665647817176904 37.0 35.0 37.0 33.0 37.0 5 35.53095606947067 37.0 35.0 37.0 33.0 37.0 6 35.61738795171768 37.0 35.0 37.0 33.0 37.0 7 35.570918814882944 37.0 35.0 37.0 33.0 37.0 8 35.58562748663921 37.0 35.0 37.0 33.0 37.0 9 37.14595870562759 39.0 37.0 39.0 33.0 39.0 10-11 37.05113273456571 39.0 37.0 39.0 33.0 39.0 12-13 37.03334080438012 39.0 37.0 39.0 33.0 39.0 14-15 38.07830837694024 40.0 37.0 41.0 33.0 41.0 16-17 38.13991780300127 40.0 37.0 41.0 33.0 41.0 18-19 38.18475453546734 40.0 37.0 41.0 33.0 41.0 20-21 38.117001625049454 40.0 37.0 41.0 33.0 41.0 22-23 38.036221609054444 40.0 37.0 41.0 33.0 41.0 24-25 37.87212684056786 40.0 37.0 41.0 32.5 41.0 26-27 37.64938087086857 39.5 37.0 41.0 32.0 41.0 28-29 37.5567203420733 39.0 37.0 41.0 32.0 41.0 30-31 37.42742478049445 39.0 36.0 41.0 32.0 41.0 32-33 37.182446059429274 39.0 36.0 40.5 31.0 41.0 34-35 37.13863243456254 39.0 36.0 41.0 31.0 41.0 36-37 36.99400283977198 39.0 35.5 40.5 30.5 41.0 38-39 36.77386147827629 39.0 35.0 40.0 30.0 41.0 40-41 36.952955050621185 39.0 36.0 41.0 30.5 41.0 42-43 36.84919227532472 39.0 36.0 41.0 30.0 41.0 44-45 36.649860488846464 39.0 35.0 41.0 30.0 41.0 46-47 36.54271580679951 39.0 35.0 41.0 30.0 41.0 48-49 36.2992417468159 39.0 35.0 41.0 30.0 41.0 50-51 36.06683432005345 39.0 35.0 40.0 29.0 41.0 52-53 35.97045820355777 39.0 35.0 40.0 28.0 41.0 54-55 35.81607811850397 38.5 35.0 40.0 28.0 41.0 56-57 35.46146836650401 38.0 34.0 40.0 27.5 41.0 58-59 35.16838403967629 38.0 34.0 40.0 27.0 41.0 60-61 34.65906400558045 38.0 33.5 40.0 26.0 41.0 62-63 34.500733943249465 37.0 33.0 40.0 26.0 41.0 64-65 34.07557525124782 36.5 33.0 39.5 26.0 41.0 66-67 33.644567658651354 36.0 32.5 39.0 25.5 40.0 68-69 33.26448821781082 36.0 32.0 39.0 25.0 40.0 70-71 32.71900193008494 35.0 31.5 37.5 23.5 40.0 72-73 32.25057174798495 35.0 31.0 37.0 22.5 39.0 74-75 31.911447772737723 35.0 31.0 37.0 22.0 39.0 76-77 30.924813978676937 34.0 29.5 35.5 21.5 38.0 78-79 31.36702368979904 34.0 31.0 36.0 22.0 37.5 80-81 31.100729839980243 34.0 31.0 35.5 20.5 37.0 82-83 30.75618800095691 34.0 30.5 35.0 20.0 37.0 84-85 30.39932416059112 34.0 30.0 35.0 19.5 36.0 86-87 30.276869058485524 34.0 30.0 35.0 18.5 36.0 88-89 29.96926515867652 34.0 30.0 35.0 17.5 36.0 90-91 29.62606075315894 34.0 30.0 35.0 11.5 35.0 92-93 29.398070534423717 34.0 29.0 35.0 7.0 35.0 94-95 28.99599137694102 34.0 29.0 35.0 2.0 35.0 96-97 28.583194557671447 34.0 29.0 35.0 2.0 35.0 98-99 28.30522768111869 34.0 29.0 35.0 2.0 35.0 100-101 27.284770367892925 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.11152101492252342 1101 2 0.21330291155329562 1101 3 0.17518894590709877 1101 4 0.11478293183719757 1101 5 0.10346530407768739 1101 6 0.1416584746019609 1101 7 0.18686837994094674 1101 8 0.09457930415005222 1101 9 0.22021115241857103 1101 10-11 0.2578617697247907 1101 12-13 0.18640950144756374 1101 14-15 0.3660887858573929 1101 16-17 0.3042921535886194 1101 18-19 0.18953318226437688 1101 20-21 0.14968991105591556 1101 22-23 0.18509901090467906 1101 24-25 0.2913884638913302 1101 26-27 0.23569507979705406 1101 28-29 0.2864573302131532 1101 30-31 0.2524358972461016 1101 32-33 0.26083349209120144 1101 34-35 0.28581807105630475 1101 36-37 0.30575557818301746 1101 38-39 0.3819342848117202 1101 40-41 0.46157943921706135 1101 42-43 0.3559899567285285 1101 44-45 0.4730813361209272 1101 46-47 0.3534656979194821 1101 48-49 0.44870306808584814 1101 50-51 0.4481046094705974 1101 52-53 0.3968417680567953 1101 54-55 0.3291080484427056 1101 56-57 0.38354945537135166 1101 58-59 0.4300785718951943 1101 60-61 0.3209161891680097 1101 62-63 0.3249438434856202 1101 64-65 0.3597382965743563 1101 66-67 0.21178084375193862 1101 68-69 0.3442056484096838 1101 70-71 0.3864758929322605 1101 72-73 0.3181261671480371 1101 74-75 0.2146545033900118 1101 76-77 0.11927965109548921 1101 78-79 0.17982265991539848 1101 80-81 0.13484754455913261 1101 82-83 0.2947541235326767 1101 84-85 0.3800020934819628 1101 86-87 0.1141687571060821 1101 88-89 0.1639664977889126 1101 90-91 0.15032106575241144 1101 92-93 0.2704327896835039 1101 94-95 0.22663711028964784 1101 96-97 0.08254572383276582 1101 98-99 0.17098816540714523 1101 100-101 0.3196414019339535 1102 1 0.06767715161456778 1102 2 0.13847406891639835 1102 3 0.12120368316926644 1102 4 0.09849422841379862 1102 5 0.06916456785002367 1102 6 0.0806003808635154 1102 7 -0.022735050958758052 1102 8 0.0367578542911815 1102 9 0.061359199883838755 1102 10-11 0.09568850013938857 1102 12-13 0.1666617480666659 1102 14-15 0.16842443094304116 1102 16-17 0.15515346878378722 1102 18-19 0.08673328037723138 1102 20-21 0.1927272346084692 1102 22-23 0.14552010186368136 1102 24-25 0.09702318541985733 1102 26-27 0.0904716951324005 1102 28-29 0.06917072249045475 1102 30-31 0.13594245314182984 1102 32-33 0.16350906898079387 1102 34-35 0.16476466690887293 1102 36-37 0.08111241284888848 1102 38-39 0.16492075998040434 1102 40-41 0.014437579724322802 1102 42-43 0.11738302579261273 1102 44-45 0.2409205276303581 1102 46-47 0.08917435993722478 1102 48-49 0.047067080749016554 1102 50-51 0.1861444049078429 1102 52-53 0.07310255634018858 1102 54-55 0.07087727608358563 1102 56-57 0.08169992642741875 1102 58-59 0.16777713286432316 1102 60-61 0.11378226004056557 1102 62-63 0.015339173466337286 1102 64-65 -0.04518040447246818 1102 66-67 -0.09264382985124797 1102 68-69 -0.1194910074925275 1102 70-71 -0.009425353685578841 1102 72-73 -0.010392074598470913 1102 74-75 -0.04911894615367274 1102 76-77 -0.16000678124413525 1102 78-79 -0.04359615995982935 1102 80-81 0.06640398041912476 1102 82-83 -0.008289884911132361 1102 84-85 0.0019546536300971695 1102 86-87 -0.006022188043495191 1102 88-89 -0.0029875493027162747 1102 90-91 0.1761930882116154 1102 92-93 0.03321542015008916 1102 94-95 0.08664964128352182 1102 96-97 0.3013248351997504 1102 98-99 0.06787753045685463 1102 100-101 0.3835523302060899 1103 1 -0.06127680099340438 1103 2 0.06961391019128271 1103 3 0.04577442095529349 1103 4 0.021145217837521102 1103 5 0.07238650501706445 1103 6 -0.027398991856685484 1103 7 0.07584394644334225 1103 8 0.13495711412693367 1103 9 0.12311677568068546 1103 10-11 0.12016966586892153 1103 12-13 0.07216006938038078 1103 14-15 0.12143919729103914 1103 16-17 0.10158576630622917 1103 18-19 -0.056022285920995785 1103 20-21 -0.01861494652376905 1103 22-23 0.08078756412159294 1103 24-25 0.11593837693136066 1103 26-27 6.866842684090102E-4 1103 28-29 0.04104146785827112 1103 30-31 0.06750771713396375 1103 32-33 0.12884756678823095 1103 34-35 0.1831370637462939 1103 36-37 0.02847842089335728 1103 38-39 0.017783064287570483 1103 40-41 -0.09381962249111808 1103 42-43 0.25866735202735214 1103 44-45 0.26836628704392496 1103 46-47 0.09520633889484031 1103 48-49 0.2269456392496636 1103 50-51 0.2929900655637283 1103 52-53 0.24631399888284733 1103 54-55 0.45020991323138304 1103 56-57 0.35064387366334415 1103 58-59 0.3357388331554816 1103 60-61 0.3371869410512929 1103 62-63 0.3423367161884343 1103 64-65 0.3610763389474059 1103 66-67 0.42994456119549085 1103 68-69 0.3491621748184386 1103 70-71 0.34800182061979257 1103 72-73 0.434969294945148 1103 74-75 0.2346698400534315 1103 76-77 0.32224567165965823 1103 78-79 0.4406490241448502 1103 80-81 0.45277237381169044 1103 82-83 0.5138391435633558 1103 84-85 0.4240530471328299 1103 86-87 0.6147710519798082 1103 88-89 0.46642648517564567 1103 90-91 0.5420090768480001 1103 92-93 0.5531438661875008 1103 94-95 0.5089806726468638 1103 96-97 0.7946046629051722 1103 98-99 0.6896346137612994 1103 100-101 0.9223811443815784 1104 1 -0.002512722087722352 1104 2 -0.00846560582990108 1104 3 0.05696113221629773 1104 4 -0.04609281988867053 1104 5 -0.06656612892306413 1104 6 -0.053410526870365516 1104 7 -0.09869240019058623 1104 8 -0.051802288867207835 1104 9 -0.1036618647217793 1104 10-11 -0.053970801783627564 1104 12-13 -0.016156322981977667 1104 14-15 -0.05594059596659662 1104 16-17 0.08151895880001803 1104 18-19 -0.10427008726196618 1104 20-21 -0.07962271660797882 1104 22-23 -0.10287532372228014 1104 24-25 -0.015501037752557068 1104 26-27 -0.038252266362306386 1104 28-29 0.09424030088361235 1104 30-31 0.05912745748064907 1104 32-33 0.031696231486243676 1104 34-35 -0.046561436566221914 1104 36-37 -0.0427472433731495 1104 38-39 0.04565226606597861 1104 40-41 0.09739267187353562 1104 42-43 0.049656496978833786 1104 44-45 0.2622121903628596 1104 46-47 0.12902794181941601 1104 48-49 0.1993836253443817 1104 50-51 0.15765042304543186 1104 52-53 0.12442139848378986 1104 54-55 0.059626146418239045 1104 56-57 0.08877691452603642 1104 58-59 -0.0830642149107419 1104 60-61 -0.016192763676791344 1104 62-63 -0.15112235496525983 1104 64-65 -0.07344732921989561 1104 66-67 -0.11873853739719209 1104 68-69 -0.20567154712411195 1104 70-71 -0.1808253743680055 1104 72-73 -0.06320308664373897 1104 74-75 0.07500767078523651 1104 76-77 -0.1514640383042618 1104 78-79 -0.23050631728118276 1104 80-81 -0.170470544671808 1104 82-83 -0.019712993480400343 1104 84-85 -0.034893793613967716 1104 86-87 0.05130917146660252 1104 88-89 -0.047263124701412806 1104 90-91 -0.24394519181949903 1104 92-93 -0.21397783917611335 1104 94-95 -0.07532975721744961 1104 96-97 -0.08242810417824842 1104 98-99 -0.07192895393465193 1104 100-101 -0.04182456212387464 1105 1 -0.020480782391874897 1105 2 -0.07513948637646095 1105 3 0.012227960046867992 1105 4 0.02116512613533672 1105 5 -0.029147119282022516 1105 6 0.011717060138572322 1105 7 0.09279292473971168 1105 8 0.0032443053565813784 1105 9 -0.054821795145038266 1105 10-11 -0.028980935182175926 1105 12-13 -0.016441238888511123 1105 14-15 0.12470619791668014 1105 16-17 0.20514350967129502 1105 18-19 0.12540480613921545 1105 20-21 -0.01062502860796144 1105 22-23 0.07829657915366539 1105 24-25 0.016070685520425343 1105 26-27 0.1892279739148819 1105 28-29 0.06904485487982726 1105 30-31 0.1509726029761893 1105 32-33 0.15872820838371382 1105 34-35 -0.019790663584437596 1105 36-37 0.04245061911864667 1105 38-39 0.10402662631938142 1105 40-41 0.22471093952012922 1105 42-43 0.27678218961644063 1105 44-45 0.32648227996701706 1105 46-47 0.24634067127659875 1105 48-49 0.14673200689587418 1105 50-51 0.3647571946142065 1105 52-53 0.4017823024249978 1105 54-55 0.2286090184676297 1105 56-57 0.2631101886602636 1105 58-59 0.41921179654477214 1105 60-61 0.42366426239960475 1105 62-63 0.31484099076106986 1105 64-65 0.17202151312706349 1105 66-67 0.19698500343385206 1105 68-69 0.21992884663080048 1105 70-71 0.3749386450635299 1105 72-73 0.3300655385104463 1105 74-75 0.4817758752375916 1105 76-77 0.3912249046941163 1105 78-79 0.45736195856593653 1105 80-81 0.38064869041106775 1105 82-83 0.3447724935195744 1105 84-85 0.33224780837848655 1105 86-87 0.19949350583090464 1105 88-89 0.409739320577426 1105 90-91 0.4334491288837192 1105 92-93 0.5138279153002934 1105 94-95 0.4792271504283896 1105 96-97 0.534317947516481 1105 98-99 0.6701858386274822 1105 100-101 0.7150457688702829 1106 1 -0.047964317959486635 1106 2 -0.03824803477199623 1106 3 -0.0336397272921225 1106 4 -0.10338059986552395 1106 5 -0.012761806211180726 1106 6 -0.031212950050935717 1106 7 -0.11640120361367678 1106 8 0.025209351169642957 1106 9 -0.0529275660883215 1106 10-11 -0.0817755354707117 1106 12-13 -0.1708766877863681 1106 14-15 -0.0983410942494487 1106 16-17 -0.0836360466911259 1106 18-19 -0.09427345961827172 1106 20-21 -0.03965685311270306 1106 22-23 -0.11784666852555858 1106 24-25 -0.05517046817828941 1106 26-27 -0.006781201277803461 1106 28-29 -0.10524391540732836 1106 30-31 0.047199836309324894 1106 32-33 -0.1601416830407416 1106 34-35 -0.0068045018513060995 1106 36-37 -0.011536233170552634 1106 38-39 -0.002442155978016558 1106 40-41 0.11858296617307928 1106 42-43 0.10804815831167502 1106 44-45 0.17517270626455428 1106 46-47 0.13338840836475896 1106 48-49 -0.04676421954295762 1106 50-51 -0.05947844324309415 1106 52-53 0.07998863766447784 1106 54-55 0.18418186551176774 1106 56-57 0.06357838302497498 1106 58-59 0.047098906849022626 1106 60-61 0.10248468273174893 1106 62-63 -0.01295332814076744 1106 64-65 0.021155868324477467 1106 66-67 -0.026917042214357423 1106 68-69 -0.09894236896636244 1106 70-71 0.108916320835867 1106 72-73 0.05370854287292559 1106 74-75 0.0692196061736432 1106 76-77 0.12021342875135588 1106 78-79 0.0495314963700757 1106 80-81 0.05628182703854634 1106 82-83 0.16719960939859035 1106 84-85 0.10533674577623486 1106 86-87 0.1818768776645605 1106 88-89 0.02402386675364454 1106 90-91 0.20230038257733796 1106 92-93 0.22180777728177148 1106 94-95 0.2652108816425134 1106 96-97 0.166127091855941 1106 98-99 0.04227323799863214 1106 100-101 0.12219411660068502 1107 1 -0.12081819442182251 1107 2 -0.09821598130153575 1107 3 -0.023021420738519538 1107 4 -0.06467661176294826 1107 5 -0.11520043125453583 1107 6 -0.03444649165030711 1107 7 -0.03842441078827363 1107 8 -0.0607561814984976 1107 9 0.026542518981102603 1107 10-11 0.0550643510580997 1107 12-13 -0.13351890467993854 1107 14-15 0.023478313861957645 1107 16-17 0.018608103873226867 1107 18-19 -0.025240964206687977 1107 20-21 -0.17171521515862054 1107 22-23 -0.17349668715773703 1107 24-25 -0.03798062270142566 1107 26-27 0.015172504624473504 1107 28-29 0.05251734874132552 1107 30-31 -0.055749517780633084 1107 32-33 -0.06325381821427101 1107 34-35 -0.11443669948764779 1107 36-37 -0.07107412783908984 1107 38-39 0.20217319048717997 1107 40-41 0.15046964716800915 1107 42-43 0.15205365658925984 1107 44-45 0.09111090040681091 1107 46-47 0.10451344917043315 1107 48-49 0.11024402923219156 1107 50-51 0.151613582759758 1107 52-53 0.12305760120212028 1107 54-55 0.22030800522563965 1107 56-57 0.35052632602850053 1107 58-59 0.36156481878486346 1107 60-61 0.19524900921652488 1107 62-63 0.2863278636124207 1107 64-65 0.3196511793664456 1107 66-67 0.3480188831155928 1107 68-69 0.19345434130675443 1107 70-71 0.21553292009037506 1107 72-73 0.27976329941296285 1107 74-75 0.0753945530371034 1107 76-77 0.09760929488633252 1107 78-79 0.1290155088694327 1107 80-81 0.2629972431069305 1107 82-83 0.19013129263720074 1107 84-85 0.2841627381650653 1107 86-87 0.35047147429731496 1107 88-89 0.33147460016870767 1107 90-91 0.2625811018169948 1107 92-93 0.1662137231393963 1107 94-95 0.2450135605327084 1107 96-97 0.26541123894183016 1107 98-99 0.38372091281420495 1107 100-101 0.3471634677624884 1108 1 -0.011588300581042432 1108 2 -0.08039413471922785 1108 3 -0.024958782071159646 1108 4 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Count 2 2.0 3 8.0 4 15.0 5 48.0 6 87.0 7 261.0 8 666.0 9 1183.0 10 2066.0 11 3322.0 12 4782.0 13 6586.0 14 8432.0 15 10913.0 16 13349.0 17 16494.0 18 15851.0 19 13411.0 20 13315.0 21 13749.0 22 14826.0 23 16429.0 24 19007.0 25 23366.0 26 29495.0 27 37281.0 28 47388.0 29 59471.0 30 73124.0 31 90875.0 32 112374.0 33 141568.0 34 185892.0 35 250512.0 36 363097.0 37 493861.0 38 431421.0 39 68758.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.754252704136185 20.04052573465481 14.775084883676348 20.430136677532655 2 14.22390248944424 24.617768671025363 36.09293481269455 25.06539402683584 3 26.95057376992047 23.776658403016736 29.07565615471535 20.197111672347443 4 12.035585408774647 15.792863872882268 41.770429054862255 30.40112166348083 5 11.606759558008056 40.56663847280906 34.90036987502443 12.926232094158452 6 27.87168844883262 38.90607616751558 16.508574671370717 16.713660712281083 7 26.24300024851876 33.7680476103102 21.69537793819238 18.293574202978665 8 23.88203333248171 29.189108839603207 19.42588295772936 27.502974870185724 9 21.405090995801505 22.272816305927364 25.054716707970332 31.2673759903008 10-11 25.55852849023693 24.41114215660088 27.53040096682313 22.499928386339054 12-13 30.755073296596162 22.534713761109554 25.371660455954594 21.33855248633969 14-15 23.243860938456574 25.9165550995665 29.812580820420294 21.027003141556634 16-17 20.206955738112324 28.880666866410714 29.23540610365168 21.676971291825282 18-19 19.20256446584338 33.30931372435166 27.668015326097645 19.820106483707313 20-21 22.539865417980288 26.97799816456187 25.416559476847873 25.06557694060997 22-23 20.162884304066182 27.818674984690162 27.059125012677555 24.9593156985661 24-25 24.66833197460928 27.267346570634682 26.522003200549992 21.542318254206045 26-27 23.503799199125417 23.92156012598445 31.716615515164275 20.858025159725855 28-29 19.64747077177761 32.01861097878177 28.422315752878006 19.91160249656261 30-31 21.63620694928319 27.08376483578001 26.554541199498725 24.725487015438077 32-33 22.12455071785459 27.493250968730603 28.43982837223644 21.94236994117837 34-35 25.600926618267223 25.523076127985277 28.081418992616204 20.794578261131296 36-37 23.723132551531858 24.5675277039936 31.02328465416639 20.686055090308155 38-39 21.395315785325963 28.012695039264894 30.848593010370536 19.74339616503861 40-41 21.57798027335936 29.364403589174714 27.733162895860165 21.324453241605763 42-43 19.75664451264541 30.1527940295445 25.380765586452465 24.709795871357628 44-45 22.401814270696203 25.31063249578555 26.20185658458044 26.085696648937805 46-47 25.03492756860378 28.75252157883062 26.475000222591877 19.73755062997372 48-49 24.099343393404205 27.221650597960267 27.734931765095723 20.944074243539806 50-51 18.48955430029606 28.151013601397302 32.080751328551784 21.278680769754853 52-53 21.72127149586109 31.249961289643274 25.511925112492296 21.516842102003338 54-55 19.90027420528951 26.955539335887835 27.78940058263059 25.354785876192064 56-57 23.665031770716585 25.249657477103522 27.504849907415256 23.580460844764637 58-59 27.81698667280399 25.218443797117533 25.537246600646547 21.427322929431924 60-61 22.83910566729445 27.14028000642625 31.147104993699564 18.873509332579733 62-63 20.882810884971033 27.653728498218072 29.670097609205477 21.79336300760542 64-65 19.64061022357473 32.51403769479099 26.997634706770846 20.84771737486344 66-67 22.177719128689315 27.045096554159542 25.202877574158055 25.574306742993087 68-69 20.16016874866423 28.354007471031167 26.515849612974566 24.969974167330037 70-71 24.478780727082107 27.482382765346408 25.85085765212456 22.18797885544692 72-73 23.347098640269483 24.26437286175157 30.79890651746251 21.589621980516437 74-75 19.975554546941364 31.95357492976529 27.3516748425707 20.719195680722642 76-77 21.577300449987582 26.928763611258265 26.202810482045496 25.291125456708656 78-79 22.02460423606286 27.573138182050442 28.00628654489712 22.395971036989582 80-81 25.028945635625256 26.330483093077785 27.02721063945177 21.613360631845193 82-83 22.99272227487895 25.24006660489797 30.126000007354953 21.641211112868127 84-85 20.986833280848654 28.1538663725202 29.63441823393277 21.22488211269838 86-87 21.765917771904853 29.392980645540913 26.700265442978537 22.1408361395757 88-89 20.132218249919823 29.591536731345453 24.901555352843616 25.374689665891108 90-91 22.18454627766872 24.89404003504063 26.23092207718306 26.690491610107586 92-93 24.234717380213013 28.461801095810234 26.625869916470986 20.677611607505767 94-95 23.054726986374042 27.017729670763995 27.890712870148803 22.036830472713152 96-97 18.04834257538926 28.032588917004215 31.648594510046514 22.27047399756001 98-99 21.202941737227647 30.814397440351815 25.563305515418573 22.419355307001965 100-101 19.289704039801254 27.00672380783889 27.474914682142952 26.228657470216906 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 141.0 1 100.5 2 112.0 3 170.0 4 150.5 5 135.0 6 201.5 7 375.5 8 575.0 9 714.0 10 745.5 11 759.0 12 858.5 13 1007.0 14 1183.0 15 1313.5 16 1422.0 17 1599.5 18 1766.5 19 1926.5 20 2166.5 21 2481.0 22 2878.0 23 3476.5 24 4185.0 25 5035.5 26 6185.0 27 7893.5 28 10615.0 29 13716.0 30 17284.0 31 22295.0 32 28726.0 33 35846.5 34 43251.5 35 50603.0 36 57248.5 37 63894.5 38 69407.0 39 71638.5 40 73872.5 41 75887.5 42 77359.0 43 81498.5 44 83172.0 45 84920.0 46 96357.5 47 179270.0 48 352247.0 49 344340.5 50 178305.5 51 96735.0 52 65178.5 53 51509.5 54 43791.5 55 37434.0 56 30798.0 57 23780.0 58 18437.0 59 14881.0 60 11790.5 61 8756.0 62 6385.5 63 4554.5 64 2979.5 65 1886.5 66 1216.0 67 761.0 68 441.0 69 276.0 70 175.5 71 106.5 72 62.0 73 39.0 74 26.5 75 20.0 76 14.5 77 9.0 78 5.0 79 2.5 80 4.0 81 5.0 82 2.5 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04811663813733255 2 0.004606500352648892 3 0.03530359934130916 4 0.0 5 3.871008699704952E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.935504349852476E-5 14-15 0.0012580778274041092 16-17 0.0 18-19 0.0 20-21 0.010509788619698944 22-23 0.0 24-25 0.0 26-27 0.005032311309616437 28-29 0.012174322360572074 30-31 0.010722694098182717 32-33 0.006096838702035299 34-35 0.005787158006058903 36-37 0.019761499411993778 38-39 0.040161715259438874 40-41 0.005787158006058903 42-43 0.009813007053752052 44-45 0.008883964965822864 46-47 0.0039871389606961 48-49 9.290420879291884E-4 50-51 0.0016258236538760796 52-53 6.967815659468913E-4 54-55 8.70976957433614E-4 56-57 0.010335593228212222 58-59 0.007916212790896627 60-61 0.005845223136554477 62-63 0.005729092875563329 64-65 0.06180065389078956 66-67 0.027639002115893353 68-69 0.020516346108436244 70-71 0.012542068187044043 72-73 0.0 74-75 1.354853044896733E-4 76-77 4.83876087463119E-4 78-79 4.258109569675447E-4 80-81 5.806513049557428E-4 82-83 4.83876087463119E-4 84-85 4.83876087463119E-4 86-87 0.002980676698772813 88-89 5.8065130495574274E-5 90-91 7.354916529439408E-4 92-93 4.645210439645942E-4 94-95 0.001451628262389357 96-97 0.0037355233952152786 98-99 0.005361347049091358 100-101 0.0066387799199939925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2583306.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.41896517610782 #Duplication Level Percentage of deduplicated Percentage of total 1 79.27976257467108 32.83685725250397 2 12.653874430038892 10.48220768721244 3 3.359088579314789 4.1738991867030215 4 1.369810722303154 2.2694457041973375 5 0.7171391591498323 1.4851580929625072 6 0.46081021841150305 1.145176943350841 7 0.3132970893972063 0.908350886486318 8 0.23423843619556375 0.7761530905352008 9 0.1846894762231429 0.6884682285671955 >10 1.216133280087591 9.830844026596797 >50 0.12833652704145188 3.6774874763209064 >100 0.07203528725019841 5.2969585700579715 >500 0.0048763429183979285 1.3727954715392592 >1k 0.003563481363444639 3.3038522975498217 >5k 8.439824281842567E-4 2.4939014157026014 >10k+ 0.0015004132056609006 19.258443669713888 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 113539 4.395104567558005 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 51727 2.0023566700963804 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 48860 1.8913748506758394 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 40790 1.5789844486096498 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 31908 1.235161455901856 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 28123 1.0886437766180237 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 27649 1.0702951953814221 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 27635 1.0697532541634633 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 22976 0.8894029588442096 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 20776 0.8042407674507007 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 18661 0.7223689334519411 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 18546 0.7179172734472803 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 12600 0.4877470961628239 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 10812 0.41853346061209934 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 10687 0.4136946997374682 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 10539 0.40796560686190486 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 9496 0.3675909861239822 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 9123 0.3531521236740828 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 8523 0.329926071475853 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8221 0.31823562520274407 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6850 0.2651640959297892 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 6417 0.24840262826006676 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 5270 0.20400215847445097 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 5259 0.20357634751748344 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5049 0.19544722924810298 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4760 0.18426001410595572 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4681 0.1812019172331888 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 4151 0.16068557112475254 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3864 0.14957577615659934 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3844 0.14880157441665834 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3685 0.14264667058412747 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 3506 0.1357175650116556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3328 0.12882716952618078 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3189 0.12344646743359092 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3077 0.11911093768992136 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2966 0.11481411803324887 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2743 0.10618176863290683 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2648 0.10250431036818712 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.871008699704952E-5 0.0 10-11 0.0 0.0 0.0 1.1613026099114855E-4 0.0 12-13 0.0 1.935504349852476E-5 0.0 2.709706089793466E-4 0.0 14-15 0.0 7.742017399409904E-5 0.0 4.258109569675447E-4 0.0 16-17 0.0 1.1613026099114855E-4 0.0 6.774265224483665E-4 0.0 18-19 0.0 1.1613026099114855E-4 0.0 0.0010645273924188617 0.0 20-21 0.0 1.1613026099114855E-4 0.0 0.0018000190453628024 0.0 22-23 0.0 1.1613026099114855E-4 0.0 0.002670996002796417 0.0 24-25 0.0 1.1613026099114855E-4 0.0 0.003541972960230031 0.0 26-27 0.0 1.1613026099114855E-4 0.0 0.004877470961628239 0.0 28-29 0.0 1.1613026099114855E-4 0.0 0.00801298800838925 0.0 30-31 0.0 1.1613026099114855E-4 0.0 0.014671122971881766 0.0 32-33 0.0 1.1613026099114855E-4 0.0 0.028142233246854997 0.0 34-35 0.0 1.5484034798819807E-4 0.0 0.04099398212987544 0.0 36-37 0.0 1.5484034798819807E-4 0.0 0.05061343874864224 0.0 38-39 0.0 1.5484034798819807E-4 0.0 0.0594974037144651 0.0 40-41 0.0 1.5484034798819807E-4 0.0 0.06648457441743255 0.0 42-43 0.0 1.5484034798819807E-4 0.0 0.07554273477474213 0.0 44-45 0.0 1.5484034798819807E-4 0.0 0.08311055678266532 0.0 46-47 0.0 1.5484034798819807E-4 0.0 0.09441390218580377 0.0 48-49 0.0 1.5484034798819807E-4 0.0 0.10629789889389797 0.0 50-51 0.0 1.5484034798819807E-4 0.0 0.11574316012117805 0.0 52-53 0.0 1.5484034798819807E-4 0.0 0.1331239891828533 0.0 54-55 0.0 1.935504349852476E-4 0.0 0.17694380766351336 0.0 56-57 0.0 2.322605219822971E-4 0.0 0.27731906324686273 0.0 58-59 0.0 2.322605219822971E-4 0.0 0.366623233949056 0.0 60-61 0.0 2.322605219822971E-4 0.0 0.43572073923878935 0.0 62-63 0.0 2.322605219822971E-4 0.0 0.5236894119395843 0.0 64-65 0.0 2.322605219822971E-4 0.0 0.6305292520514411 0.0 66-67 0.0 2.322605219822971E-4 0.0 0.8559380886352604 0.0 68-69 0.0 2.322605219822971E-4 0.0 1.1099923895968964 0.0 70-71 0.0 2.322605219822971E-4 0.0 1.2577487916646344 0.0 72-73 0.0 2.5161556548082185E-4 0.0 1.3880275894532046 0.0 74-75 0.0 2.709706089793466E-4 0.0 1.5599584408506 0.0 76-77 0.0 3.0968069597639615E-4 0.0 1.70926324639822 0.0 78-79 0.0 3.0968069597639615E-4 0.0 1.8156772755531092 0.0 80-81 0.0 3.0968069597639615E-4 0.0 1.9385043815947474 0.0 82-83 0.0 3.0968069597639615E-4 0.0 2.1020544991572816 0.0 84-85 0.0 3.0968069597639615E-4 0.0 2.4668002938869806 0.0 86-87 0.0 3.0968069597639615E-4 0.0 2.986541276952866 0.0 88-89 0.0 3.0968069597639615E-4 0.0 3.5809927279230567 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1985 0.0 36.136433 2 ACCTGGG 2125 0.0 32.636044 3 GTACCTG 2425 0.0 29.787146 1 TACGCAG 1825 0.0 26.280643 4 GAGTACG 1925 0.0 26.1681 1 GTACGCA 1880 0.0 25.519205 3 AGTACGC 1900 0.0 25.252048 2 ACGTGCG 190 1.1954398E-8 24.994284 9 CGCAGTG 2040 0.0 24.441994 6 GTACACG 255 1.8189894E-11 24.227032 1 CCGGTCG 355 0.0 24.079002 9 GTACTAG 485 0.0 23.516169 1 ACGCAGT 2140 0.0 23.077942 5 CCCGGTC 410 0.0 22.00631 8 CCCCGGT 425 0.0 21.229618 7 CCTGGGG 1620 0.0 20.226074 4 GTACCCG 260 3.2679054E-7 18.277792 1 GTAGGCA 1310 0.0 18.13019 3 GTATAGA 820 0.0 17.96573 1 TATACTG 1085 0.0 17.50684 5 >>END_MODULE