##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003781_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4206725 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.839339391094022 31.0 31.0 34.0 30.0 34.0 2 32.01558029108154 31.0 31.0 34.0 30.0 34.0 3 32.084726004195666 33.0 31.0 34.0 30.0 34.0 4 35.76219743387077 37.0 35.0 37.0 35.0 37.0 5 35.64095846531447 37.0 35.0 37.0 33.0 37.0 6 35.728344971444535 37.0 35.0 37.0 33.0 37.0 7 35.69690864033185 37.0 35.0 37.0 33.0 37.0 8 35.7097961002918 37.0 35.0 37.0 33.0 37.0 9 37.308328212564405 39.0 37.0 39.0 34.0 39.0 10-11 37.21243627762689 39.0 37.0 39.0 33.0 39.0 12-13 37.19614189185174 39.0 37.0 39.0 33.0 39.0 14-15 38.27529824269473 40.0 38.0 41.0 33.0 41.0 16-17 38.342404602154886 40.0 38.0 41.0 33.0 41.0 18-19 38.402624369313415 40.0 38.0 41.0 33.5 41.0 20-21 38.33623281769072 40.0 38.0 41.0 33.5 41.0 22-23 38.25378019718427 40.0 38.0 41.0 33.5 41.0 24-25 38.09990360672495 40.0 37.5 41.0 33.0 41.0 26-27 37.88157474044536 40.0 37.0 41.0 33.0 41.0 28-29 37.78704312737344 40.0 37.0 41.0 33.0 41.0 30-31 37.66351306538935 40.0 37.0 41.0 32.5 41.0 32-33 37.42925957841314 39.0 36.0 41.0 32.0 41.0 34-35 37.35791975943281 39.0 36.0 41.0 31.5 41.0 36-37 37.20187544942918 39.0 36.0 41.0 31.5 41.0 38-39 36.97993082504799 39.0 36.0 40.0 31.0 41.0 40-41 37.14984982379404 39.0 36.0 41.0 31.0 41.0 42-43 37.06339515894193 39.0 36.0 41.0 31.0 41.0 44-45 36.85463216635269 39.0 36.0 41.0 30.5 41.0 46-47 36.74464042693544 39.0 36.0 41.0 30.0 41.0 48-49 36.49603230541573 39.0 36.0 41.0 30.0 41.0 50-51 36.26211209432516 39.0 35.0 41.0 29.0 41.0 52-53 36.16773356946318 39.0 35.0 41.0 29.0 41.0 54-55 36.03342933041737 39.0 35.0 40.5 28.5 41.0 56-57 35.69203763022304 38.5 34.5 40.0 28.0 41.0 58-59 35.40852801169556 38.0 34.0 40.0 28.0 41.0 60-61 34.919633087496806 38.0 34.0 40.0 26.0 41.0 62-63 34.765587244233934 38.0 34.0 40.0 26.0 41.0 64-65 34.36026148607289 37.0 33.5 39.5 26.0 41.0 66-67 33.933620334107886 36.5 33.0 39.0 26.0 41.0 68-69 33.55166061484884 36.0 33.0 39.0 25.5 40.0 70-71 32.991944446095246 35.5 32.0 38.5 24.5 40.0 72-73 32.531100440366316 35.0 31.5 37.0 23.5 40.0 74-75 32.190569029351806 35.0 31.0 37.0 23.0 39.0 76-77 31.1812653548782 34.0 30.5 36.0 21.5 39.0 78-79 31.61374869999822 34.5 31.0 36.0 23.0 39.0 80-81 31.313048630466692 34.0 31.0 36.0 21.0 37.5 82-83 30.95607949176616 34.0 31.0 35.0 20.5 37.0 84-85 30.603982195175583 34.0 31.0 35.0 20.0 37.0 86-87 30.477572933814308 34.0 31.0 35.0 19.0 36.0 88-89 30.166310966369323 34.0 30.5 35.0 17.5 36.0 90-91 29.817825743303878 34.0 30.0 35.0 13.0 36.0 92-93 29.58595130416179 34.0 30.0 35.0 7.0 35.0 94-95 29.20001319316095 34.0 29.0 35.0 2.0 35.0 96-97 28.832382910696563 34.0 29.0 35.0 2.0 35.0 98-99 28.568552734015178 34.0 29.0 35.0 2.0 35.0 100-101 27.615761073043757 33.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.09430457025435857 1101 2 0.2123415556630306 1101 3 0.19386931715865074 1101 4 0.09271232417456332 1101 5 0.11060789068066867 1101 6 0.1407419794081548 1101 7 0.07203373275051916 1101 8 0.08619804144551324 1101 9 0.16035725800293932 1101 10-11 0.24197941786132304 1101 12-13 0.23433475184987884 1101 14-15 0.3462547326262637 1101 16-17 0.28740845274999316 1101 18-19 0.1955622519726674 1101 20-21 0.20559382692209027 1101 22-23 0.15111761538710766 1101 24-25 0.27401999608447625 1101 26-27 0.33628448535210964 1101 28-29 0.2702479185156861 1101 30-31 0.29825200541019825 1101 32-33 0.27154498085979384 1101 34-35 0.26502812518985763 1101 36-37 0.3618631185900725 1101 38-39 0.32271520850189717 1101 40-41 0.40943467989989557 1101 42-43 0.39400879225635066 1101 44-45 0.4529470957374926 1101 46-47 0.4241010022460401 1101 48-49 0.3712129674152749 1101 50-51 0.3667073181239502 1101 52-53 0.2688989644870432 1101 54-55 0.3119682210212673 1101 56-57 0.2124408113168741 1101 58-59 0.32869922558901266 1101 60-61 0.3795180402124032 1101 62-63 0.30033772085116084 1101 64-65 0.28443182765072805 1101 66-67 0.2923124056483175 1101 68-69 0.3366134629884385 1101 70-71 0.44896037687176005 1101 72-73 0.43149445733739356 1101 74-75 0.42149242437712786 1101 76-77 0.35872728706653945 1101 78-79 0.33602485536895443 1101 80-81 0.33772286585339373 1101 82-83 0.4427580669817779 1101 84-85 0.4279110569320572 1101 86-87 0.32467426373062125 1101 88-89 0.45887901325919955 1101 90-91 0.4193204811926847 1101 92-93 0.4946436188352692 1101 94-95 0.525742220970205 1101 96-97 0.4977716925437434 1101 98-99 0.49284840545526976 1101 100-101 0.513046936450877 1102 1 0.01023274559353382 1102 2 0.16123136952298722 1102 3 0.11174060220218252 1102 4 0.07522960191371908 1102 5 0.09900626094369613 1102 6 -0.004015197544383398 1102 7 0.030312305325068678 1102 8 0.013488255877362576 1102 9 0.09228920100852633 1102 10-11 0.10751283655597632 1102 12-13 0.03418194034536981 1102 14-15 0.06882947189248512 1102 16-17 0.04584245753660099 1102 18-19 -0.0025231584833775855 1102 20-21 -0.023590301268491487 1102 22-23 0.05012853196507194 1102 24-25 0.07433110305714763 1102 26-27 0.007916751929293753 1102 28-29 0.05727428626472175 1102 30-31 0.11444303239154152 1102 32-33 0.06852326365389416 1102 34-35 0.09965766362824269 1102 36-37 0.04394366695331797 1102 38-39 0.056916009834992565 1102 40-41 0.0655538367869326 1102 42-43 0.10813479634820311 1102 44-45 0.26394063258759815 1102 46-47 0.12887418621994584 1102 48-49 0.1386328341058629 1102 50-51 0.3275276516435994 1102 52-53 0.1267427362424698 1102 54-55 0.09561979186035074 1102 56-57 0.1552224539645266 1102 58-59 0.12744414922526204 1102 60-61 0.14556647230756425 1102 62-63 -0.010519663438969928 1102 64-65 -0.16824162532726916 1102 66-67 -0.10340357756779639 1102 68-69 -0.06947371890026233 1102 70-71 -0.0056873917525379625 1102 72-73 0.07304292398092826 1102 74-75 -0.06477033545152722 1102 76-77 -0.021145572531210632 1102 78-79 0.04185413123965631 1102 80-81 0.07552069181380006 1102 82-83 0.002360262507622224 1102 84-85 -0.10858137862929951 1102 86-87 -0.10336137339592 1102 88-89 -0.23149817458791233 1102 90-91 -0.30860103388152993 1102 92-93 -0.31552584451481636 1102 94-95 -0.2201706584289731 1102 96-97 0.034593106765086645 1102 98-99 0.012158819803925525 1102 100-101 0.3403271649813071 1103 1 -0.005810838670111451 1103 2 0.010065907728197487 1103 3 0.011340033549409156 1103 4 -0.0013544424056277649 1103 5 0.002607916098988028 1103 6 0.009027878255970734 1103 7 0.08844345447374025 1103 8 0.03435751106304963 1103 9 0.06339268135718612 1103 10-11 0.03339468151035163 1103 12-13 0.01968340924961609 1103 14-15 0.015495859068209938 1103 16-17 0.03057783062207875 1103 18-19 -0.027039585038259872 1103 20-21 -0.004877516616701882 1103 22-23 0.032934459077672784 1103 24-25 -0.023608090224392697 1103 26-27 -0.06257451339114795 1103 28-29 -0.05249327929306702 1103 30-31 -0.03170411378018656 1103 32-33 0.023027980651072255 1103 34-35 0.09884077094800858 1103 36-37 -0.023768580296746222 1103 38-39 0.057267166714183304 1103 40-41 -0.030962549699339093 1103 42-43 0.16315880381679904 1103 44-45 0.2238799026673135 1103 46-47 0.1334485935430081 1103 48-49 0.16669632232973441 1103 50-51 0.3342652694371182 1103 52-53 0.24272133886068303 1103 54-55 0.2875776067827971 1103 56-57 0.1989905818432618 1103 58-59 0.224220054186965 1103 60-61 0.23287452865268676 1103 62-63 0.09776016932897846 1103 64-65 0.1492635852929638 1103 66-67 0.10049202539033075 1103 68-69 0.07970930494322914 1103 70-71 0.13786852705880648 1103 72-73 0.2947929590255072 1103 74-75 0.15562565251774174 1103 76-77 0.18650225823875033 1103 78-79 0.3145523980680558 1103 80-81 0.37090595617297595 1103 82-83 0.32257248517855786 1103 84-85 0.10438271624452256 1103 86-87 0.20431759711772202 1103 88-89 0.16572861358064017 1103 90-91 0.21507521938648466 1103 92-93 0.23279504868878576 1103 94-95 0.3159965214313054 1103 96-97 0.27030887782712654 1103 98-99 0.24055965731215423 1103 100-101 0.36258187905681183 1104 1 0.04799328440791939 1104 2 0.015196656159204736 1104 3 -0.04354238076415129 1104 4 -0.02274789784208764 1104 5 -0.08314158371172908 1104 6 -0.007206048512124141 1104 7 -0.08006133915260705 1104 8 -0.07740258087388696 1104 9 -0.11559198932697967 1104 10-11 -0.023610103625514967 1104 12-13 -0.04366173709133392 1104 14-15 -0.03586725038609728 1104 16-17 -0.04061273974853208 1104 18-19 -0.13053784473913055 1104 20-21 -0.11363132235047146 1104 22-23 -0.007211162680334837 1104 24-25 0.10964147451354478 1104 26-27 -0.09459811493382375 1104 28-29 0.02941977117735206 1104 30-31 -0.004490089451458346 1104 32-33 0.13160195672389108 1104 34-35 0.0281236386033612 1104 36-37 0.09893842764734728 1104 38-39 0.15840238788126015 1104 40-41 0.23702750220247992 1104 42-43 0.10728721711549127 1104 44-45 0.18555474017446727 1104 46-47 0.20453631046233767 1104 48-49 0.15600293461736214 1104 50-51 0.1875198023557232 1104 52-53 0.12125316093813865 1104 54-55 0.11267095838565666 1104 56-57 0.27908785529972135 1104 58-59 0.3282550871649974 1104 60-61 0.20967057153861646 1104 62-63 -0.03905499212503116 1104 64-65 0.06287508305397438 1104 66-67 0.1295001947324934 1104 68-69 0.1398259766542438 1104 70-71 0.07528227999176096 1104 72-73 0.03330464992001936 1104 74-75 0.053617035427507176 1104 76-77 -0.029186891724140906 1104 78-79 0.10902807208747944 1104 80-81 0.14243073314798238 1104 82-83 0.16666953363723636 1104 84-85 0.0886418617424205 1104 86-87 0.15495031284339333 1104 88-89 0.11200427606299712 1104 90-91 0.008581792268287813 1104 92-93 0.0800180616912094 1104 94-95 0.17037629295742462 1104 96-97 0.16292022925217964 1104 98-99 0.10044553774019249 1104 100-101 0.3082912388644665 1105 1 -0.0060638093613221145 1105 2 0.017713191404602924 1105 3 0.054791432571306586 1105 4 0.05132429116619619 1105 5 -0.01816800038005084 1105 6 0.038710788638056215 1105 7 0.03644038885501999 1105 8 0.04866997558429631 1105 9 0.03538398739457449 1105 10-11 0.016686488028405222 1105 12-13 0.03540988087994634 1105 14-15 0.10091026366637834 1105 16-17 0.13208225697420772 1105 18-19 0.014679720359083603 1105 20-21 -0.0769919744250771 1105 22-23 0.09127087199898654 1105 24-25 0.0581649714448389 1105 26-27 0.07342133011751883 1105 28-29 -0.0018114839123128945 1105 30-31 0.08252564634212689 1105 32-33 0.0954247763556495 1105 34-35 0.007369546999093757 1105 36-37 0.06326276673408415 1105 38-39 0.049155436722301715 1105 40-41 0.10482337385322182 1105 42-43 0.13505711899643558 1105 44-45 0.18065077370178528 1105 46-47 0.1392207256211364 1105 48-49 0.14597155240987547 1105 50-51 0.16572865789825642 1105 52-53 0.27965001179320836 1105 54-55 0.2550191866812881 1105 56-57 0.1824294148762533 1105 58-59 0.14463553187868428 1105 60-61 0.20538990436877214 1105 62-63 0.07909392935985693 1105 64-65 0.11881846101095306 1105 66-67 0.11565651983627845 1105 68-69 0.07664619434347486 1105 70-71 0.1685548434017008 1105 72-73 0.25045835321912335 1105 74-75 0.29367466050943847 1105 76-77 0.3049722935301915 1105 78-79 0.29232412249763584 1105 80-81 0.2413185765150203 1105 82-83 0.30185298548143535 1105 84-85 0.28133606397890176 1105 86-87 0.27313064136346554 1105 88-89 0.26973090327017957 1105 90-91 0.25635065138600766 1105 92-93 0.3632449669685869 1105 94-95 0.32677203153171774 1105 96-97 0.36099496558205146 1105 98-99 0.3954931572607201 1105 100-101 0.44086567229645013 1106 1 -0.055835003858188514 1106 2 -0.027978375954788248 1106 3 0.014553864850135767 1106 4 -0.07101128254098654 1106 5 0.07525141992802986 1106 6 -0.05241576575761542 1106 7 -0.04986239132107073 1106 8 -0.060963268501851076 1106 9 -0.06610988739166146 1106 10-11 -0.07496304121084307 1106 12-13 -0.09212747400784593 1106 14-15 -0.008657199639429791 1106 16-17 -0.014532165662338059 1106 18-19 -0.011413311817371152 1106 20-21 -0.04136704739010355 1106 22-23 0.027188525110091177 1106 24-25 0.06528234924525123 1106 26-27 0.017483003046883994 1106 28-29 -0.004575527515576994 1106 30-31 -0.03387833552790198 1106 32-33 -0.041557492661119966 1106 34-35 -0.08717982097942212 1106 36-37 -0.04798176970866308 1106 38-39 -0.040290997645087145 1106 40-41 0.12042195008901047 1106 42-43 0.15739561130479984 1106 44-45 0.11070630409821547 1106 46-47 0.017117068453508466 1106 48-49 0.0067678430281503665 1106 50-51 -0.06448678618455261 1106 52-53 -0.01584549612678643 1106 54-55 -0.03542024560909596 1106 56-57 -0.07315605010501969 1106 58-59 -0.03164506051396643 1106 60-61 -0.11445148408998307 1106 62-63 -0.16033258795995664 1106 64-65 -0.04324883231039678 1106 66-67 -0.10087312224700185 1106 68-69 -0.20400774551153233 1106 70-71 -0.07415888480019817 1106 72-73 0.03683788298436497 1106 74-75 -0.06057132689503675 1106 76-77 -0.10775498852727239 1106 78-79 -0.12941823750252013 1106 80-81 -0.05019814033448711 1106 82-83 0.05000968036766196 1106 84-85 -0.041685958310235804 1106 86-87 0.07891620692422663 1106 88-89 0.10261837483787417 1106 90-91 0.015149047245827774 1106 92-93 0.04064347982902916 1106 94-95 0.08988668573200087 1106 96-97 0.09804166833246342 1106 98-99 -0.021613181171289853 1106 100-101 0.049495334521282075 1107 1 -0.00676123123112049 1107 2 0.05486941082996566 1107 3 0.052104706381740584 1107 4 0.014159619694673609 1107 5 0.02275936836991832 1107 6 0.015957880365810695 1107 7 -0.009215515539530372 1107 8 0.04848684791741675 1107 9 0.06445262313187783 1107 10-11 0.08114934768858717 1107 12-13 -0.026814339098642392 1107 14-15 0.12645375584695273 1107 16-17 0.06159552156423587 1107 18-19 0.16259705934760404 1107 20-21 0.10120891790609932 1107 22-23 0.0803527492752778 1107 24-25 0.11138532909445331 1107 26-27 0.10661072254346493 1107 28-29 0.14515832947675023 1107 30-31 0.23781970071183878 1107 32-33 0.11537391040510414 1107 34-35 0.12716513554324393 1107 36-37 0.15841910397585224 1107 38-39 0.21415448906864043 1107 40-41 0.2838158131137476 1107 42-43 0.2737477689843999 1107 44-45 0.34333114805059495 1107 46-47 0.2163319443037537 1107 48-49 0.2005902114543403 1107 50-51 0.23132127439563277 1107 52-53 0.22856417998379897 1107 54-55 0.22569225129077353 1107 56-57 0.37006317490985907 1107 58-59 0.2933944897670031 1107 60-61 0.19443028978207622 1107 62-63 0.31696998138347965 1107 64-65 0.34562668664472795 1107 66-67 0.30046870512561696 1107 68-69 0.27870554819735105 1107 70-71 0.2571640463993603 1107 72-73 0.3400208445749868 1107 74-75 0.22923779729427451 1107 76-77 0.23631440162418116 1107 78-79 0.3590440626673903 1107 80-81 0.42019025396444576 1107 82-83 0.4855583369072711 1107 84-85 0.5655353478600809 1107 86-87 0.39002090306954784 1107 88-89 0.2953527689697282 1107 90-91 0.28447406662735375 1107 92-93 0.23885261786322332 1107 94-95 0.35497343610811427 1107 96-97 0.2942007498551007 1107 98-99 0.3689062215628667 1107 100-101 0.4392805294568696 1108 1 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-1.3336626169323758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 20.0 5 37.0 6 126.0 7 374.0 8 934.0 9 1911.0 10 3163.0 11 5509.0 12 8401.0 13 11835.0 14 14078.0 15 17975.0 16 22175.0 17 28246.0 18 26722.0 19 21269.0 20 20406.0 21 21032.0 22 22221.0 23 24392.0 24 28118.0 25 34462.0 26 42796.0 27 54633.0 28 69755.0 29 87183.0 30 109046.0 31 135265.0 32 168388.0 33 215114.0 34 284003.0 35 388425.0 36 579170.0 37 826892.0 38 770479.0 39 162059.0 40 102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.97338375731607 19.06313375333235 14.011444097233205 19.95203839211837 2 14.028084410606073 24.82184259579088 36.29290439418875 24.857168599414294 3 27.098789786059747 24.104444300170346 29.43348555836097 19.363280355408936 4 11.785557648764776 15.963320635411158 42.87820097581848 29.372920740005586 5 11.477693455122452 40.70266062079171 34.84216344068129 12.977482483404549 6 26.82350284366104 39.190130089321265 17.000921144120426 16.985445922897267 7 25.958744629135495 34.08024056718706 22.239319185352024 17.72169561832542 8 22.984982379404407 28.890074820674037 19.89535802791958 28.229584772001974 9 21.08421634406813 23.030148155631753 25.77306574591874 30.11256975438138 10-11 25.287830794739374 24.239592557155508 27.868377419489033 22.604199228616086 12-13 31.055373367093566 22.589855836768013 25.724016393277 20.630754402861417 14-15 23.236036323868213 25.99112287659401 30.660699455897923 20.11214134363986 16-17 19.659830390624535 29.192923236009012 29.952718563728315 21.19452780963814 18-19 18.64250693829523 33.91260422300007 28.058073679643904 19.386815159060788 20-21 22.064048394391946 27.32067406474497 25.702067566949122 24.913209973913958 22-23 19.57009312469914 28.145314942146204 27.406747529253757 24.877844403900898 24-25 24.66701531476386 27.31771152143294 26.90738044440747 21.10789271939573 26-27 23.48097459158251 23.936331564633612 32.61522698313079 19.967466860653087 28-29 19.28992118979188 32.256636126690694 28.908420726545053 19.54502195697237 30-31 21.195457782504054 27.44336641132176 27.092366719551986 24.268809086622202 32-33 22.006139825266853 27.766442729969764 28.80166904732387 21.425748397439516 34-35 25.41865853823787 25.482583857109674 28.56793735176113 20.530820252891317 36-37 23.634185299716254 24.653901040401077 31.82771825526085 19.884195404621817 38-39 21.341812894424923 28.18773104239479 31.39210950386886 19.078346559311424 40-41 20.923098136009553 29.949797126187093 28.43175303538093 20.69535170242242 42-43 19.21755023075986 30.490506601841915 25.55732740559351 24.73461576180471 44-45 22.087748842880828 25.742696541462706 26.287648349412013 25.881906266244453 46-47 25.009309988687754 28.909603902623637 26.726222859436582 19.354863249252023 48-49 23.898247065367777 27.518161676959867 28.135242686596968 20.44834857107539 50-51 18.351675611080083 28.588279524022735 32.707586917269516 20.352457947627666 52-53 21.427752152501423 31.61336071679406 26.031755955504533 20.92713117519999 54-55 19.358450862759373 27.45769404211448 28.214193768620692 24.969661326505456 56-57 23.454166197133542 25.444392300512614 27.852597980270577 23.248843522083273 58-59 27.783534883544014 25.518246498903096 25.78402033474737 20.91419828280552 60-61 22.815901366558233 27.199343103027534 31.63174544006118 18.35301009035305 62-63 20.599116843974226 27.960747353164823 30.206591564289724 21.23354423857123 64-65 19.402826191494828 32.97878376020464 27.358312491993818 20.260077556306705 66-67 21.790374207950798 27.377265218508917 25.455053028388097 25.377307545152185 68-69 19.861686170435995 28.839507000635205 26.80157085858314 24.497235970345663 70-71 24.580803963398758 27.742650116367063 25.910900083616912 21.765645836617264 72-73 23.243790823105048 24.693317853328377 31.24652787434952 20.81636344921705 74-75 19.837029063867387 32.38597181021422 27.67753163345814 20.099467492460242 76-77 21.407769459782497 27.321024961275835 26.453392179478524 24.81781339946314 78-79 22.081836852364024 27.873299918974006 28.186243076330246 21.858620152331728 80-81 25.051210473679948 26.593503300362592 27.347153643782395 21.008132582175072 82-83 22.965352027465922 25.435335690305926 30.7594431934542 20.839869088773952 84-85 21.0470820975921 28.461065949833742 29.995635483990597 20.496216468583565 86-87 21.356325183700164 29.865406600867665 27.129796238963667 21.648471976468503 88-89 19.802800904173875 29.797314435284743 25.22839090758034 25.171493752961037 90-91 22.01544728473238 25.127106939558715 26.54701055032764 26.310435225381262 92-93 24.263345396222956 28.49463956753786 27.05229878930141 20.189716246937774 94-95 22.781268174817722 27.28096228219786 28.352947427239215 21.584822115745204 96-97 17.64804192293881 28.08096834704483 32.4598000967339 21.811189633282453 98-99 20.894446880619206 30.989244522856385 26.224518579441597 21.89179001708281 100-101 18.968222174937917 27.200845887117573 28.013674937325185 25.81725700061932 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 198.0 1 150.5 2 246.5 3 377.5 4 316.0 5 256.5 6 357.0 7 661.5 8 1062.5 9 1371.5 10 1465.0 11 1544.0 12 1736.5 13 1966.0 14 2292.5 15 2619.5 16 2865.0 17 3225.5 18 3615.0 19 3979.5 20 4465.5 21 5180.0 22 5987.5 23 7351.0 24 9265.5 25 11567.0 26 15133.0 27 20145.0 28 27082.5 29 35967.0 30 45541.0 31 55412.0 32 65335.0 33 74299.0 34 82434.0 35 90352.5 36 97952.5 37 105500.5 38 111960.0 39 115284.5 40 117304.5 41 119519.0 42 120759.5 43 123195.5 44 124572.5 45 127592.0 46 143311.0 47 277238.0 48 578229.5 49 564922.5 50 273079.0 51 141140.0 52 95239.0 53 75481.5 54 63644.5 55 54130.5 56 45067.5 57 35576.0 58 27915.5 59 22233.5 60 17580.0 61 13249.0 62 9766.5 63 6969.0 64 4590.0 65 3022.0 66 1958.5 67 1208.5 68 719.0 69 414.0 70 245.0 71 150.5 72 95.5 73 62.0 74 43.5 75 25.5 76 15.5 77 13.0 78 9.0 79 4.5 80 1.5 81 3.0 82 3.5 83 2.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05020532599587565 2 0.004564120545079604 3 0.04198040043026345 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.1885730586144805E-5 14-15 0.0016521165514741276 16-17 0.0 18-19 0.0 20-21 0.010756586180461047 22-23 0.0 24-25 0.0 26-27 0.0058240079872109544 28-29 0.012931674877725546 30-31 0.010780357641633338 32-33 0.0060260654071754155 34-35 0.006037951137761561 36-37 0.020134427612929297 38-39 0.04442886093100928 40-41 0.00695315239289471 42-43 0.012182873850798425 44-45 0.011445958554457446 46-47 0.005265378649662148 48-49 0.0015451449761988245 50-51 0.0022701745419536575 52-53 0.0011766873280283357 54-55 0.001141030136269901 56-57 0.009960242231189347 58-59 0.00782081072568328 60-61 0.006691666319999524 62-63 0.007927782300958584 64-65 0.06989998157711759 66-67 0.031758672126178916 68-69 0.023759575441703464 70-71 0.01516619222792077 72-73 1.9017168937831688E-4 74-75 5.705150681349505E-4 76-77 0.0011648015974421908 78-79 0.0012004587892006254 80-81 0.001568916437371114 82-83 0.0013668590174066525 84-85 0.0017353166655771413 86-87 0.00660846620589651 88-89 2.8525753406747527E-4 90-91 7.369152963409779E-4 92-93 5.110864152042265E-4 94-95 0.004362063125115142 96-97 0.008094182529164612 98-99 0.010685271796944179 100-101 0.011648015974421909 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4206725.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.76849032412683 #Duplication Level Percentage of deduplicated Percentage of total 1 76.8137843961654 32.85209594706964 2 14.632303365049701 12.516030497756331 3 3.9938578121594897 5.124338075858444 4 1.5333133403177592 2.623099870369387 5 0.8094667067568013 1.7309834507815531 6 0.4595229868279746 1.1791862649519655 7 0.30313248229779804 0.9075163045257345 8 0.2102546330003274 0.7193818589661872 9 0.1511694963504041 0.5818762027768846 >10 0.94962821530633 7.672795398912352 >50 0.08581653454740608 2.5286932942663305 >100 0.04837677538735949 3.7948316578609558 >500 0.004381581130863781 1.29643169240956 >1k 0.0029950048215050596 2.7986405438632187 >5k 7.764827315013118E-4 2.363816206377677 >10k+ 0.0012201871495020613 21.31028273325369 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 192370 4.572915985713352 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 94912 2.2561969227843512 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 79068 1.8795618919705948 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 69544 1.6531624957657083 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 52891 1.2572963528635697 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 44513 1.0581390511621274 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 42211 1.0034171475435167 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 41120 0.9774824834045487 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 37358 0.8880542464743951 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 32704 0.7774218661785594 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 30969 0.7361783810446368 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 30823 0.7327077477134826 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 18361 0.4364677985844095 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 17127 0.4071338154978041 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 16783 0.39895643285453647 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 15576 0.37026427921958294 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 15151 0.36016140822135984 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 15065 0.35811706256054293 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 14751 0.350652823752444 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13046 0.31012248245369023 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12176 0.28944131123379824 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 11864 0.2820246153480439 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 9759 0.23198568958037427 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 9696 0.23048808752652003 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8729 0.207501084572916 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 8632 0.2051952528392039 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8120 0.19302426471899162 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 8010 0.19040940399003975 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7128 0.16944297523608035 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 6368 0.15137666474514022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6080 0.1445304839275208 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 5774 0.1372564168088002 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 5640 0.13407104101171338 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 5299 0.12596497275196264 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 5259 0.12501411430507106 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 5158 0.1226131967266698 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4745 0.1127955832625142 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4539 0.10789866226102252 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 4464 0.1061158026731008 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.377146117228961E-5 0.0 3 0.0 0.0 0.0 2.377146117228961E-5 0.0 4 0.0 0.0 0.0 2.377146117228961E-5 0.0 5 0.0 2.377146117228961E-5 0.0 2.377146117228961E-5 0.0 6 0.0 2.377146117228961E-5 0.0 2.377146117228961E-5 0.0 7 0.0 2.377146117228961E-5 0.0 2.377146117228961E-5 0.0 8 0.0 2.377146117228961E-5 0.0 1.1885730586144804E-4 0.0 9 0.0 2.377146117228961E-5 0.0 1.4262876703373764E-4 0.0 10-11 0.0 2.377146117228961E-5 0.0 1.7828595879217204E-4 0.0 12-13 0.0 2.377146117228961E-5 0.0 2.8525753406747527E-4 0.0 14-15 0.0 2.377146117228961E-5 0.0 4.1600057051506816E-4 0.0 16-17 0.0 2.377146117228961E-5 0.0 7.369152963409779E-4 0.0 18-19 0.0 2.377146117228961E-5 0.0 9.270869857192947E-4 0.0 20-21 0.0 2.377146117228961E-5 0.0 0.0012361159809590595 0.0 22-23 0.0 2.377146117228961E-5 0.0 0.0017234309349909967 0.0 24-25 0.0 2.377146117228961E-5 0.0 0.002175088697264499 0.0 26-27 0.0 2.377146117228961E-5 0.0 0.0026267464595380015 0.0 28-29 0.0 2.377146117228961E-5 0.0 0.003625147828774165 0.0 30-31 0.0 2.377146117228961E-5 0.0 0.005455550339040465 0.0 32-33 2.377146117228961E-5 2.377146117228961E-5 0.0 0.00848641163850739 0.0 34-35 2.377146117228961E-5 2.377146117228961E-5 0.0 0.011113158098045391 0.0 36-37 2.377146117228961E-5 2.377146117228961E-5 0.0 0.013442761292929773 0.0 38-39 2.377146117228961E-5 2.377146117228961E-5 0.0 0.015903107524261748 0.0 40-41 2.377146117228961E-5 2.377146117228961E-5 0.0 0.021132828982165463 0.0 42-43 2.377146117228961E-5 2.377146117228961E-5 0.0 0.027028151352893284 0.0 44-45 2.377146117228961E-5 2.377146117228961E-5 0.0 0.040054912075307994 0.0 46-47 2.377146117228961E-5 2.377146117228961E-5 0.0 0.06885403728553685 0.0 48-49 2.377146117228961E-5 2.377146117228961E-5 0.0 0.08513748818855524 0.0 50-51 2.377146117228961E-5 2.377146117228961E-5 0.0 0.1055809447967243 0.0 52-53 2.377146117228961E-5 2.377146117228961E-5 0.0 0.12942372035253075 0.0 54-55 2.377146117228961E-5 2.377146117228961E-5 0.0 0.15899541805085904 0.0 56-57 2.377146117228961E-5 2.377146117228961E-5 0.0 0.19106311917227772 0.0 58-59 2.377146117228961E-5 2.377146117228961E-5 0.0 0.2585859546321663 0.0 60-61 2.377146117228961E-5 2.377146117228961E-5 0.0 0.3267743910048791 0.0 62-63 4.754292234457922E-5 2.377146117228961E-5 0.0 0.4018446653869697 0.0 64-65 4.754292234457922E-5 2.377146117228961E-5 0.0 0.4396056314591517 0.0 66-67 4.754292234457922E-5 2.377146117228961E-5 0.0 0.5970440188032258 0.0 68-69 4.754292234457922E-5 2.377146117228961E-5 0.0 0.7661185363911356 0.0 70-71 4.754292234457922E-5 2.377146117228961E-5 0.0 0.8134356298545782 0.0 72-73 4.754292234457922E-5 2.377146117228961E-5 0.0 0.9622449767931109 0.0 74-75 4.754292234457922E-5 2.377146117228961E-5 0.0 1.0361029066554148 0.0 76-77 4.754292234457922E-5 2.377146117228961E-5 0.0 1.0833843429270988 0.0 78-79 4.754292234457922E-5 2.377146117228961E-5 0.0 1.1526187235913925 0.0 80-81 4.754292234457922E-5 2.377146117228961E-5 0.0 1.219737444211352 0.0 82-83 4.754292234457922E-5 2.377146117228961E-5 0.0 1.3446802441329062 0.0 84-85 4.754292234457922E-5 2.377146117228961E-5 0.0 1.625956058454023 0.0 86-87 4.754292234457922E-5 3.5657191758434416E-5 0.0 2.1814356773975003 0.0 88-89 1.6640022820602724E-4 4.754292234457922E-5 0.0 2.8615490672672923 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3745 0.0 37.929184 2 ACCTGGG 3975 0.0 34.65851 3 TACACGG 470 0.0 34.366493 2 GTACCTG 4435 0.0 33.758076 1 GAGTACG 3125 0.0 32.700108 1 AGTACGC 3060 0.0 32.447334 2 TACGCAG 3070 0.0 32.32665 4 GTACACG 505 0.0 31.999878 1 GTACGCA 3125 0.0 31.620033 3 TATAACG 515 0.0 30.441137 2 CGCAGTG 3455 0.0 28.586966 6 ACGCAGT 3450 0.0 28.490763 5 ATAACGC 610 0.0 26.478786 3 ACACGGG 600 0.0 25.336565 3 TAACGCA 650 0.0 24.8381 4 CTAACGC 160 1.8594528E-6 23.753029 3 CCTGGGG 3025 0.0 21.976347 4 TCACGCA 525 0.0 21.707247 4 GTATAAC 1125 0.0 20.279139 1 GGCGTCG 365 5.456968E-12 19.51422 8 >>END_MODULE