FastQCFastQC Report
Wed 25 May 2016
SRR1003780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5754228
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4608108.008198493351323No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1756283.052155736616623No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1669672.9016403242971953No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1582812.750690448831711No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1245602.164669178906362No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1016591.7666835585937852No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1011831.7584113802928907No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC977631.6989768219125136No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG887441.542239897341572No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA787321.3682460966093106No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA776061.348677876510976No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC734991.2773042708769968No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA700021.2165315660067693No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA693371.204974846321696No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC500840.8703860882815211No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT411950.7159083720700674No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA406350.7061763975984268No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG401150.6971395641604747No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC397030.6899796115134819No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG377900.6567344915773237No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT377420.6559003223368973No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT310860.5402288543311109No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC280380.4872591075640381No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG272490.4735474506745301No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA264010.4588104607603314No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG250660.43561012876097366No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC247510.43013589312067574No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA218440.37961651849735534No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT209090.36336759683488384No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC190180.33050480446725433No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG188190.32704647782465346No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA184990.3214853495551445No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC184200.32011244601360944No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA180320.31336957798682985No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG164730.2862764561988159No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG143510.24939922436163464No Hit
GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT142840.24823486313020615No Hit
GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC138580.24083161112142237No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT135670.23577446010133768No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA135540.23554853926538885No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG130610.2269809260251766No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT126670.2201337868433437No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT125880.2187608833018087No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA120810.20994997069980542No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT119930.2084206604256905No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA116950.20324185972471023No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA114870.19962712634952942No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA113270.19684656221477495No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA112670.19580385066424202No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG111650.19403124102833602No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG110980.1928668797969076No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT107000.18595022651170584No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG105690.1836736396263756No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG98820.17173459237277355No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA93640.1627325159865059No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC89720.15592013385635745No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC86210.1498202712857398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85470.14853426037341586No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT84410.14669213663414102No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC75510.13122524863456922No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC74730.12986972361887641No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC74180.12891390469755457No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC72040.12519490016732046No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC71490.12423908124599858No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA71320.12394364630668094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71280.12387413220331207No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT68340.11876484560570072No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT66220.11508059812715102No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC66160.11497632697209774No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT64460.1120219775789211No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG63010.10950209133179985No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT62020.1077816172734205No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC60680.10545289481056364No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT60590.10529648807798371No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC59430.1032805790802867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTAC25350.041.025264
CCTGGGG16850.040.5833364
GTACTAG15850.040.4846041
CTGGGGG16100.040.4091645
GCTTATG26500.039.8191951
TATTCTC18650.039.212735
TTATGTA26300.039.192793
GGTGTTC4250.035.755788
GCTTAGG1900.035.0235251
ACCTGGG29250.034.915223
TATTAAG4300.034.246952
TCTAACG8700.033.8533022
AGGTGTT4700.033.342747
TACCTGG32700.033.1219332
GTACCCG2050.032.4608271
TAACGCC9350.031.9973584
CTAACGC9300.031.667293
GACGTAC600.003957089531.658766
AGGGCTA39600.030.9392455
ATTTAGG40250.030.4676651
Produced by FastQC (version 0.11.5)