FastQCFastQC Report
Wed 25 May 2016
SRR1003779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455500
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT183781.2626588801099279No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT85310.5861216076949503No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT69410.4768807969769838No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC66440.45647543799381657No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53000.36413603572655445No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT52700.36207488835451734No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA47530.326554448643078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44160.3034008931638612No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT43720.30037787701820684No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA37930.2605977327378908No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33210.22816901408450702No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA32990.22665750601167986No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32810.22542081758845758No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA31320.2151837856406733No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT28090.19299209893507385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27610.1896942631398145No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26100.17931982136722777No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25020.1718996908278942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16160.11102713844039849No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA14770.10147715561662658No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA14680.10085881140501547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16100.051.9316832
TATAACG2850.050.005992
ACCTGGG18450.044.544633
TAACGCA3300.043.1662184
GTACCTG20900.041.8433231
ATAACGC3950.037.2829443
GTATAAC5100.035.4134371
TACGCAG3500.031.2030094
CCTGGGG13450.031.066844
GTACACG1859.094947E-1230.8293341
GAGTACG3900.028.0296611
GTACGCA3600.027.7116533
TATCACG2750.027.6396732
AGTACGC3650.027.332042
TCACGCA2800.027.1330534
TACACGG1858.960342E-925.6787512
ACGCAGT4400.023.741425
TATACGC1150.003721163820.6546483
ACACGGG2351.13095666E-720.2151853
CACGCAG4000.020.1802065
Produced by FastQC (version 0.11.5)